RchiOBHm_Chr1g0351401

carboxylesterase 8

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
1
OG0005485
Physical Location & Seq
Forward (+)
44674335 .. 44674878
544 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ57718

Sequence Viewer

Length: 336 bp
ATGTGGGCCCTGAGCCTGCCGGAGGGGGCTGATCGTGACCACGAGTATTGTAATCCATCTATCAACGGCGGTGATGGAAGGATCGGACGGTTGCCGTGGTGTTTGGTGAGCGGGAATGGCGGGGACCCGCTGGTGGATAGGCAGGAGGAGATCGCGGCACTGTTGGAGTCACGCGGGGTGCACGTGACGGCCCGGTTTGAGGAGGGTGGTTTTCATGGTGCGGAGCTATTCGACCCCAACACGGCCGAGGCTTTCTATGAGATTGTTAAGGAATTTATCGGCTCTTGTTGCGCACGCGGTAGCGATGATGTTGCCGTGTTAAAATCAGCCATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0009056 GO:0016787
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

111

Amino Acids

11.97

Weight (kDa)

4.53

Isoelectric Point (pI)

28.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydrolase_3 PF07859 1 - 74 3.3e-11 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 292
AccBSI CCGCTC 1 cut(s) 111
AccII CGCG 3 cut(s) 155, 174, 297
AciI CCGC 8 cut(s) 69, 111, 120, 128, 155, 174, 221, 297
AclWI GGATC 1 cut(s) 89
AcoI YGGCCR 1 cut(s) 243
AcsI RAATTY 1 cut(s) 272
AcvI CACGTG 1 cut(s) 184
AfiI CCNNNNNNNGG 4 cut(s) 22, 133, 199, 241
AluBI AGCT 1 cut(s) 226
AluI AGCT 1 cut(s) 226
Alw21I GWGCWC 1 cut(s) 183
Alw44I GTGCAC 1 cut(s) 179
AlwI GGATC 1 cut(s) 89
AoxI GGCC 3 cut(s) 6, 189, 243
ApaI GGGCCC 1 cut(s) 10
ApaLI GTGCAC 1 cut(s) 179
ApoI RAATTY 1 cut(s) 272
AspLEI GCGC 1 cut(s) 293
AspS9I GGNCC 4 cut(s) 6, 7, 124, 190
AsuC2I CCSGG 1 cut(s) 193
AsuHPI GGTGA 2 cut(s) 83, 118
AvaII GGWCC 1 cut(s) 124
BaeGI GKGCMC 2 cut(s) 10, 183
BanII GRGCYC 1 cut(s) 10
BauI CACGAG 1 cut(s) 41
BbrPI CACGTG 1 cut(s) 184
Bbv12I GWGCWC 1 cut(s) 183
BccI CCATC 2 cut(s) 64, 68
BceAI ACGGC 5 cut(s) 79, 82, 204, 258, 299
BcgI CGANNNNNNTGC 2 cut(s) 293, 327
BcnI CCSGG 1 cut(s) 193
BisI GCNGC 1 cut(s) 156
BlsI GCNGC 1 cut(s) 157
Bme1390I CCNGG 1 cut(s) 193
Bme18I GGWCC 1 cut(s) 124
BmgT120I GGNCC 4 cut(s) 6, 7, 124, 190
BmiI GGNNCC 3 cut(s) 8, 125, 126
BmrFI CCNGG 1 cut(s) 193
Bpu10I CCTNAGC 1 cut(s) 11
BpuMI CCSGG 1 cut(s) 193
BsaAI YACGTR 1 cut(s) 184
BsaJI CCNNGG 2 cut(s) 95, 246
Bsc4I CCNNNNNNNGG 4 cut(s) 22, 133, 199, 241
BseDI CCNNGG 2 cut(s) 95, 246
BseLI CCNNNNNNNGG 4 cut(s) 22, 133, 199, 241
BseRI GAGGAG 2 cut(s) 161, 215
BseSI GKGCMC 2 cut(s) 10, 183
BseX3I CGGCCG 1 cut(s) 243
Bsh1236I CGCG 3 cut(s) 155, 174, 297
Bsh1285I CGRYCG 1 cut(s) 246
BshFI GGCC 3 cut(s) 8, 191, 245
BsiEI CGRYCG 1 cut(s) 246
BsiHKAI GWGCWC 1 cut(s) 183
BsiSI CCGG 2 cut(s) 20, 193
BslFI GGGAC 1 cut(s) 137
BslI CCNNNNNNNGG 4 cut(s) 22, 133, 199, 241
BsmFI GGGAC 1 cut(s) 137
BsnI GGCC 3 cut(s) 8, 191, 245
Bsp120I GGGCCC 1 cut(s) 6
Bsp1286I GDGCHC 2 cut(s) 10, 183
Bsp143I GATC 3 cut(s) 31, 81, 150
BspACI CCGC 8 cut(s) 69, 111, 120, 128, 155, 174, 221, 297
BspANI GGCC 3 cut(s) 8, 191, 245
BspFNI CGCG 3 cut(s) 155, 174, 297
BspLI GGNNCC 3 cut(s) 8, 125, 126
BspPI GGATC 1 cut(s) 89
BsrBI CCGCTC 1 cut(s) 111
BssECI CCNNGG 2 cut(s) 95, 246
BssMI GATC 3 cut(s) 31, 81, 150
BssSI CACGAG 1 cut(s) 41
Bst2BI CACGAG 1 cut(s) 41
Bst4CI ACNGT 2 cut(s) 90, 162
BstBAI YACGTR 1 cut(s) 184
BstC8I GCNNGC 2 cut(s) 17, 295
BstDEI CTNAG 1 cut(s) 11
BstDSI CCRYGG 1 cut(s) 95
BstENI CCTNNNNNAGG 1 cut(s) 20
BstFNI CGCG 3 cut(s) 155, 174, 297
BstHHI GCGC 1 cut(s) 293
BstKTI GATC 3 cut(s) 34, 84, 153
BstMBI GATC 3 cut(s) 31, 81, 150
BstMCI CGRYCG 1 cut(s) 246
BstMWI GCNNNNNNNGC 2 cut(s) 117, 288
BstSCI CCNGG 1 cut(s) 191
BstSLI GKGCMC 2 cut(s) 10, 183
BstUI CGCG 3 cut(s) 155, 174, 297
BstZI CGGCCG 1 cut(s) 243
BsuRI GGCC 3 cut(s) 8, 191, 245
BtgI CCRYGG 1 cut(s) 95
BtgZI GCGATG 1 cut(s) 318
BtsIMutI CAGTG 1 cut(s) 158
Cac8I GCNNGC 2 cut(s) 17, 295
CfoI GCGC 1 cut(s) 293
Cfr13I GGNCC 4 cut(s) 6, 7, 124, 190
CspCI CAANNNNNGTGG 2 cut(s) 29, 64
CviAII CATG 2 cut(s) 215, 331
CviJI RGCY 9 cut(s) 8, 15, 29, 191, 226, 245, 251, 282, 329
CviKI_1 RGCY 9 cut(s) 8, 15, 29, 191, 226, 245, 251, 282, 329
DdeI CTNAG 1 cut(s) 11
DpnI GATC 3 cut(s) 33, 83, 152
DpnII GATC 3 cut(s) 31, 81, 150
EaeI YGGCCR 1 cut(s) 243
EagI CGGCCG 1 cut(s) 243
EclXI CGGCCG 1 cut(s) 243
Eco24I GRGCYC 1 cut(s) 10
Eco47I GGWCC 1 cut(s) 124
Eco52I CGGCCG 1 cut(s) 243
Eco72I CACGTG 1 cut(s) 184
EcoNI CCTNNNNNAGG 1 cut(s) 20
EcoO109I RGGNCCY 2 cut(s) 7, 124
EcoT38I GRGCYC 1 cut(s) 10
FaeI CATG 2 cut(s) 218, 334
FaiI YATR 3 cut(s) 216, 258, 332
FaqI GGGAC 1 cut(s) 137
FatI CATG 2 cut(s) 214, 330
FauI CCCGC 4 cut(s) 104, 113, 135, 167
Fnu4HI GCNGC 1 cut(s) 156
FriOI GRGCYC 1 cut(s) 10
Fsp4HI GCNGC 1 cut(s) 156
FspI TGCGCA 1 cut(s) 292
GlaI GCGC 1 cut(s) 292
GluI GCNGC 1 cut(s) 156
HaeIII GGCC 3 cut(s) 8, 191, 245
HapII CCGG 2 cut(s) 20, 193
HhaI GCGC 1 cut(s) 293
Hin1II CATG 2 cut(s) 218, 334
Hin6I GCGC 1 cut(s) 291
HinP1I GCGC 1 cut(s) 291
HinfI GANTC 1 cut(s) 167
HpaII CCGG 2 cut(s) 20, 193
HphI GGTGA 2 cut(s) 83, 118
Hpy166II GTNNAC 1 cut(s) 181
Hpy188I TCNGA 1 cut(s) 86
Hpy188III TCNNGA 1 cut(s) 35
Hpy8I GTNNAC 1 cut(s) 181
HpyAV CCTTC 1 cut(s) 72
HpyCH4III ACNGT 2 cut(s) 90, 162
HpyCH4IV ACGT 1 cut(s) 183
HpyCH4V TGCA 1 cut(s) 181
HpyF10VI GCNNNNNNNGC 2 cut(s) 117, 288
HpyF3I CTNAG 1 cut(s) 11
HpySE526I ACGT 1 cut(s) 183
Hsp92II CATG 2 cut(s) 218, 334
HspAI GCGC 1 cut(s) 291
KflI GGGWCCC 1 cut(s) 124
Kzo9I GATC 3 cut(s) 31, 81, 150
LmnI GCTCC 1 cut(s) 223
LpnPI CCDG 6 cut(s) 23, 29, 33, 116, 128, 206
MaeII ACGT 1 cut(s) 183
MaeIII GTNAC 3 cut(s) 35, 168, 184
MalI GATC 3 cut(s) 33, 83, 152
MbiI CCGCTC 1 cut(s) 111
MboI GATC 3 cut(s) 31, 81, 150
MhlI GDGCHC 2 cut(s) 10, 183
MluCI AATT 1 cut(s) 272
MlyI GAGTC 1 cut(s) 176
MmeI TCCRAC 1 cut(s) 144
MnlI CCTC 5 cut(s) 16, 139, 193, 196, 241
MseI TTAA 2 cut(s) 267, 320
MspA1I CMGCKG 1 cut(s) 130
MspI CCGG 2 cut(s) 20, 193
MspR9I CCNGG 1 cut(s) 193
MvnI CGCG 3 cut(s) 155, 174, 297
MwoI GCNNNNNNNGC 2 cut(s) 117, 288
NciI CCSGG 1 cut(s) 193
NdeII GATC 3 cut(s) 31, 81, 150
NlaIII CATG 2 cut(s) 218, 334
NlaIV GGNNCC 3 cut(s) 8, 125, 126
NmeAIII GCCGAG 1 cut(s) 271
NmuCI GTSAC 3 cut(s) 35, 168, 184
NsbI TGCGCA 1 cut(s) 292
PkrI GCNGC 1 cut(s) 157
PleI GAGTC 1 cut(s) 175
PmaCI CACGTG 1 cut(s) 184
PmlI CACGTG 1 cut(s) 184
PpsI GAGTC 1 cut(s) 175
Ppu21I YACGTR 1 cut(s) 184
PpuMI RGGWCCY 1 cut(s) 124
Psp5II RGGWCCY 1 cut(s) 124
PspCI CACGTG 1 cut(s) 184
PspN4I GGNNCC 3 cut(s) 8, 125, 126
PspOMI GGGCCC 1 cut(s) 6
PspPI GGNCC 4 cut(s) 6, 7, 124, 190
PspPPI RGGWCCY 1 cut(s) 124
SaqAI TTAA 2 cut(s) 267, 320
SatI GCNGC 1 cut(s) 156
Sau3AI GATC 3 cut(s) 31, 81, 150
Sau96I GGNCC 4 cut(s) 6, 7, 124, 190
SchI GAGTC 1 cut(s) 176
ScrFI CCNGG 1 cut(s) 193
SduI GDGCHC 2 cut(s) 10, 183
SetI ASST 2 cut(s) 186, 228
SinI GGWCC 1 cut(s) 124
Sse9I AATT 1 cut(s) 272
SsiI CCGC 8 cut(s) 69, 111, 120, 128, 155, 174, 221, 297
StyD4I CCNGG 1 cut(s) 191
TaaI ACNGT 2 cut(s) 90, 162
TaiI ACGT 1 cut(s) 186
TaqI TCGA 1 cut(s) 231
TasI AATT 1 cut(s) 272
TauI GCSGC 1 cut(s) 158
Tru1I TTAA 2 cut(s) 267, 320
Tru9I TTAA 2 cut(s) 267, 320
TscAI CASTG 1 cut(s) 165
TseFI GTSAC 3 cut(s) 35, 168, 184
Tsp45I GTSAC 3 cut(s) 35, 168, 184
TspDTI ATGAA 1 cut(s) 203
TspRI CASTG 1 cut(s) 165
VneI GTGCAC 1 cut(s) 179
VpaK11BI GGWCC 1 cut(s) 124
XagI CCTNNNNNAGG 1 cut(s) 20
XapI RAATTY 1 cut(s) 272
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.