Rh1DG230200

carboxylesterase 8

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0005485
Physical Location & Seq
Forward (+)
44229538 .. 44230152
615 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG230200.1

Sequence Viewer

Length: 408 bp
ATGGGAAGTAGCGCCGGGGCTAATATCGCCTACCACGCGGCTCTACGCGCTTCCGATGTGGACCTCTCCCCGATCAAGATCCGAGGCCTGCCGGAGGGGGCTGATCGTGACCACGAGTATTGTAATCCATCTATCAACGGCGGTGATGGAAGGATCGGACGGTTGCCGTGGTGTTTGGTTAGCGGGTACGGCGGCGACCCGCTGGTGGATAGGCAGAACGAGCTCGCGGCATTGTTGGAGTCACGCGGGGTGCACGTGACGGCCCGGTTTGAGGAGGATGGTTTTCATGGTGCGGAGCTATTTGACCCCAATAAGGCCGAGGCTTTCTATGAGATTGTTAAGGATTTTATCGGCTCTTGTTGTGCATACGGCGGCGATGATGTTGTCGTTGCAAAATCAGCCATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0009056 GO:0016787
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

135

Amino Acids

14.37

Weight (kDa)

4.73

Isoelectric Point (pI)

31.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydrolase_3 PF07859 31 - 98 6.4e-09 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 4 cut(s) 38, 48, 227, 246
AciI CCGC 9 cut(s) 38, 141, 183, 192, 200, 227, 246, 293, 372
AclWI GGATC 2 cut(s) 73, 161
AcvI CACGTG 1 cut(s) 256
AfaI GTAC 1 cut(s) 188
AfiI CCNNNNNNNGG 4 cut(s) 94, 205, 271, 313
AluBI AGCT 2 cut(s) 223, 298
AluI AGCT 2 cut(s) 223, 298
Alw21I GWGCWC 2 cut(s) 225, 255
Alw44I GTGCAC 1 cut(s) 251
AlwI GGATC 2 cut(s) 73, 161
AoxI GGCC 3 cut(s) 85, 261, 315
ApaLI GTGCAC 1 cut(s) 251
AspLEI GCGC 2 cut(s) 14, 50
AspS9I GGNCC 2 cut(s) 61, 262
AsuC2I CCSGG 2 cut(s) 16, 265
AsuHPI GGTGA 1 cut(s) 155
AvaII GGWCC 1 cut(s) 61
BaeGI GKGCMC 1 cut(s) 255
BanII GRGCYC 1 cut(s) 225
BauI CACGAG 1 cut(s) 113
BbrPI CACGTG 1 cut(s) 256
Bbv12I GWGCWC 2 cut(s) 225, 255
BccI CCATC 3 cut(s) 136, 140, 272
BceAI ACGGC 5 cut(s) 151, 154, 205, 276, 385
BcnI CCSGG 2 cut(s) 16, 265
BfoI RGCGCY 1 cut(s) 15
BisI GCNGC 4 cut(s) 39, 193, 228, 373
BlsI GCNGC 4 cut(s) 40, 194, 229, 374
Bme1390I CCNGG 2 cut(s) 16, 265
Bme18I GGWCC 1 cut(s) 61
BmgT120I GGNCC 2 cut(s) 61, 262
BmrFI CCNGG 2 cut(s) 16, 265
BpuMI CCSGG 2 cut(s) 16, 265
BsaAI YACGTR 1 cut(s) 256
BsaBI GATNNNNATC 1 cut(s) 77
BsaJI CCNNGG 4 cut(s) 15, 82, 167, 318
Bsc4I CCNNNNNNNGG 4 cut(s) 94, 205, 271, 313
Bse8I GATNNNNATC 1 cut(s) 77
BseDI CCNNGG 4 cut(s) 15, 82, 167, 318
BseGI GGATG 1 cut(s) 283
BseJI GATNNNNATC 1 cut(s) 77
BseLI CCNNNNNNNGG 4 cut(s) 94, 205, 271, 313
BseRI GAGGAG 1 cut(s) 287
BseSI GKGCMC 1 cut(s) 255
Bsh1236I CGCG 4 cut(s) 38, 48, 227, 246
BshFI GGCC 3 cut(s) 87, 263, 317
BsiHKAI GWGCWC 2 cut(s) 225, 255
BsiSI CCGG 3 cut(s) 15, 92, 265
BslI CCNNNNNNNGG 4 cut(s) 94, 205, 271, 313
BsnI GGCC 3 cut(s) 87, 263, 317
Bsp1286I GDGCHC 2 cut(s) 225, 255
Bsp143I GATC 4 cut(s) 72, 78, 103, 153
BspACI CCGC 9 cut(s) 38, 141, 183, 192, 200, 227, 246, 293, 372
BspANI GGCC 3 cut(s) 87, 263, 317
BspFNI CGCG 4 cut(s) 38, 48, 227, 246
BspPI GGATC 2 cut(s) 73, 161
BssECI CCNNGG 4 cut(s) 15, 82, 167, 318
BssMI GATC 4 cut(s) 72, 78, 103, 153
BssSI CACGAG 1 cut(s) 113
Bst2BI CACGAG 1 cut(s) 113
Bst4CI ACNGT 1 cut(s) 162
BstBAI YACGTR 1 cut(s) 256
BstC8I GCNNGC 2 cut(s) 89, 225
BstDSI CCRYGG 1 cut(s) 167
BstENI CCTNNNNNAGG 1 cut(s) 92
BstF5I GGATG 1 cut(s) 283
BstFNI CGCG 4 cut(s) 38, 48, 227, 246
BstH2I RGCGCY 1 cut(s) 15
BstHHI GCGC 2 cut(s) 14, 50
BstKTI GATC 4 cut(s) 75, 81, 106, 156
BstMBI GATC 4 cut(s) 72, 78, 103, 153
BstMWI GCNNNNNNNGC 6 cut(s) 26, 35, 47, 189, 220, 398
BstSCI CCNGG 2 cut(s) 14, 263
BstSLI GKGCMC 1 cut(s) 255
BstUI CGCG 4 cut(s) 38, 48, 227, 246
BstX2I RGATCY 1 cut(s) 78
BstYI RGATCY 1 cut(s) 78
BsuRI GGCC 3 cut(s) 87, 263, 317
BtgI CCRYGG 1 cut(s) 167
BtgZI GCGATG 1 cut(s) 390
BtsCI GGATG 1 cut(s) 283
Cac8I GCNNGC 2 cut(s) 89, 225
CfoI GCGC 2 cut(s) 14, 50
Cfr13I GGNCC 2 cut(s) 61, 262
Csp6I GTAC 1 cut(s) 187
CspCI CAANNNNNGTGG 2 cut(s) 101, 136
CviAII CATG 2 cut(s) 287, 403
CviQI GTAC 1 cut(s) 187
DpnI GATC 4 cut(s) 74, 80, 105, 155
DpnII GATC 4 cut(s) 72, 78, 103, 153
Ecl136II GAGCTC 1 cut(s) 223
Eco147I AGGCCT 1 cut(s) 87
Eco24I GRGCYC 1 cut(s) 225
Eco47I GGWCC 1 cut(s) 61
Eco53kI GAGCTC 1 cut(s) 223
Eco72I CACGTG 1 cut(s) 256
EcoICRI GAGCTC 1 cut(s) 223
EcoNI CCTNNNNNAGG 1 cut(s) 92
EcoT38I GRGCYC 1 cut(s) 225
FaeI CATG 2 cut(s) 290, 406
FaiI YATR 4 cut(s) 288, 330, 367, 404
FatI CATG 2 cut(s) 286, 402
FauI CCCGC 3 cut(s) 176, 207, 239
Fnu4HI GCNGC 4 cut(s) 39, 193, 228, 373
FokI GGATG 1 cut(s) 290
FriOI GRGCYC 1 cut(s) 225
Fsp4HI GCNGC 4 cut(s) 39, 193, 228, 373
GlaI GCGC 2 cut(s) 13, 49
GluI GCNGC 4 cut(s) 39, 193, 228, 373
HaeII RGCGCY 1 cut(s) 15
HaeIII GGCC 3 cut(s) 87, 263, 317
HapII CCGG 3 cut(s) 15, 92, 265
HhaI GCGC 2 cut(s) 14, 50
Hin1II CATG 2 cut(s) 290, 406
Hin6I GCGC 2 cut(s) 12, 48
HinP1I GCGC 2 cut(s) 12, 48
HinfI GANTC 1 cut(s) 239
HpaII CCGG 3 cut(s) 15, 92, 265
HphI GGTGA 1 cut(s) 155
Hpy166II GTNNAC 2 cut(s) 61, 253
Hpy188I TCNGA 3 cut(s) 55, 83, 158
Hpy188III TCNNGA 2 cut(s) 76, 107
Hpy8I GTNNAC 2 cut(s) 61, 253
HpyAV CCTTC 1 cut(s) 144
HpyCH4III ACNGT 1 cut(s) 162
HpyCH4IV ACGT 1 cut(s) 255
HpyCH4V TGCA 3 cut(s) 253, 365, 392
HpyF10VI GCNNNNNNNGC 6 cut(s) 26, 35, 47, 189, 220, 398
HpySE526I ACGT 1 cut(s) 255
Hsp92II CATG 2 cut(s) 290, 406
HspAI GCGC 2 cut(s) 12, 48
Kzo9I GATC 4 cut(s) 72, 78, 103, 153
LmnI GCTCC 1 cut(s) 295
LpnPI CCDG 5 cut(s) 28, 101, 105, 188, 278
MaeII ACGT 1 cut(s) 255
MaeIII GTNAC 3 cut(s) 107, 240, 256
MalI GATC 4 cut(s) 74, 80, 105, 155
MboI GATC 4 cut(s) 72, 78, 103, 153
MflI RGATCY 1 cut(s) 78
MhlI GDGCHC 2 cut(s) 225, 255
MlyI GAGTC 1 cut(s) 248
MmeI TCCRAC 1 cut(s) 216
MnlI CCTC 6 cut(s) 74, 77, 88, 265, 268, 313
MseI TTAA 1 cut(s) 339
MspA1I CMGCKG 1 cut(s) 202
MspI CCGG 3 cut(s) 15, 92, 265
MspR9I CCNGG 2 cut(s) 16, 265
MvnI CGCG 4 cut(s) 38, 48, 227, 246
MwoI GCNNNNNNNGC 6 cut(s) 26, 35, 47, 189, 220, 398
NciI CCSGG 2 cut(s) 16, 265
NdeII GATC 4 cut(s) 72, 78, 103, 153
NlaIII CATG 2 cut(s) 290, 406
NmeAIII GCCGAG 1 cut(s) 343
NmuCI GTSAC 3 cut(s) 107, 240, 256
PceI AGGCCT 1 cut(s) 87
PkrI GCNGC 4 cut(s) 40, 194, 229, 374
PleI GAGTC 1 cut(s) 247
PmaCI CACGTG 1 cut(s) 256
PmlI CACGTG 1 cut(s) 256
PpsI GAGTC 1 cut(s) 247
Ppu21I YACGTR 1 cut(s) 256
Psp124BI GAGCTC 1 cut(s) 225
PspCI CACGTG 1 cut(s) 256
PspPI GGNCC 2 cut(s) 61, 262
PsuI RGATCY 1 cut(s) 78
RsaI GTAC 1 cut(s) 188
RsaNI GTAC 1 cut(s) 187
SacI GAGCTC 1 cut(s) 225
SaqAI TTAA 1 cut(s) 339
SatI GCNGC 4 cut(s) 39, 193, 228, 373
Sau3AI GATC 4 cut(s) 72, 78, 103, 153
Sau96I GGNCC 2 cut(s) 61, 262
SchI GAGTC 1 cut(s) 248
ScrFI CCNGG 2 cut(s) 16, 265
SduI GDGCHC 2 cut(s) 225, 255
SetI ASST 4 cut(s) 66, 225, 258, 300
SinI GGWCC 1 cut(s) 61
SseBI AGGCCT 1 cut(s) 87
SsiI CCGC 9 cut(s) 38, 141, 183, 192, 200, 227, 246, 293, 372
SstI GAGCTC 1 cut(s) 225
StuI AGGCCT 1 cut(s) 87
StyD4I CCNGG 2 cut(s) 14, 263
TaaI ACNGT 1 cut(s) 162
TaiI ACGT 1 cut(s) 258
TauI GCSGC 4 cut(s) 41, 195, 230, 375
Tru1I TTAA 1 cut(s) 339
Tru9I TTAA 1 cut(s) 339
TseFI GTSAC 3 cut(s) 107, 240, 256
Tsp45I GTSAC 3 cut(s) 107, 240, 256
TspDTI ATGAA 1 cut(s) 275
VneI GTGCAC 1 cut(s) 251
VpaK11BI GGWCC 1 cut(s) 61
XagI CCTNNNNNAGG 1 cut(s) 92
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.