Rmu_sc0005320.1_g000011

carboxylesterase 8

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005320.1
OG0005485
Physical Location & Seq
Reverse (-)
39347 .. 46038
6692 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0005320.1_g000011.1.cds

Sequence Viewer

Length: 1059 bp
atgttggctaccaatttctttggttcggcacggatccggttgatacgatctggcagatgggttggagatgaagtccacaaaatgttatttgccacatcatttgtcaaattcaaatttgatactctggtgctgaactctgttagtgaccgatcttcgtctcttccaagactgatcttcgtcctcatctcttccaagactggtcttcgtcttgtcacaggtctgtttagaccaaatcttcgcctgcgtcagaacctccaatcgcccagtggtcggtctccaatcgcccagtggtcggtctccgtgagctgcgtcgtccagtgtgccgcctctcagcctttccaccactgctgctctcgcctcgccctctccatcccagccctagtcctctccgtcgagtaccgtctcgcccccgagcaccgcctccccgccgcctacgacgacgccgtttcggccatcacctggctcaaaaaccaggcctcctctgccgacggagattcgtggctgaaaaactccgttgatttctccaagtgtttcatcatgggaactagcgcgggatccaatatcgcctaccacgcgggtctacgcgcttccgatatggacctctccccgatcaaaatccgagggctgatattgcaccaggcgtatttcggcggggtcaacaggaccgaatccgagatcaggttagtcaacgaccgggtgctgccgttagttgctaacgacttgatgtgggccctgagcctgccggagggggctgatcgtgaccacgagtattgtaatccatctatcaacggcggtgatggaaggatcggacggttgccgtggtgtttggtgagcgggtacggcggcgatccgctggtggataggcagaaggagatcgcggcactgttggagtcacgcggggtgcacgtgacggaccggtttgaggagggtggttttcatggtgcggagctatttgaccccaacaaggccgaggctttctatgagattgttaaggaatttatcggctcttgttgtgcggacggcggcgatgctgttgtcgttgcaaaatcgaccatatga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0009056 GO:0016787
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

352

Amino Acids

38.52

Weight (kDa)

7.6

Isoelectric Point (pI)

40.69

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 459
AccBSI CCGCTC 1 cut(s) 834
AccI GTMKAC 1 cut(s) 580
AccII CGCG 5 cut(s) 551, 575, 585, 878, 897
AclWI GGATC 6 cut(s) 28, 41, 549, 562, 812, 842
AcoI YGGCCR 1 cut(s) 450
AcsI RAATTY 3 cut(s) 107, 113, 995
AcvI CACGTG 1 cut(s) 907
AcyI GRCGYC 1 cut(s) 441
AfaI GTAC 2 cut(s) 398, 839
AfiI CCNNNNNNNGG 7 cut(s) 270, 292, 459, 678, 745, 922, 964
AgeI ACCGGT 1 cut(s) 915
AgsI TTSAA 1 cut(s) 112
AjnI CCWGG 3 cut(s) 458, 471, 636
AluBI AGCT 2 cut(s) 306, 949
AluI AGCT 2 cut(s) 306, 949
Alw21I GWGCWC 2 cut(s) 417, 906
Alw26I GTCTC 4 cut(s) 162, 279, 301, 407
Alw44I GTGCAC 1 cut(s) 902
AlwI GGATC 6 cut(s) 28, 41, 549, 562, 812, 842
Ama87I CYCGRG 1 cut(s) 410
AoxI GGCC 4 cut(s) 450, 474, 729, 966
ApaI GGGCCC 1 cut(s) 733
ApaLI GTGCAC 1 cut(s) 902
ApeKI GCWGC 3 cut(s) 306, 348, 700
ApoI RAATTY 3 cut(s) 107, 113, 995
ArsI GACNNNNNNTTYG 2 cut(s) 219, 251
AsiGI ACCGGT 1 cut(s) 915
AspLEI GCGC 2 cut(s) 551, 587
AspS9I GGNCC 5 cut(s) 598, 663, 729, 730, 913
AsuC2I CCSGG 1 cut(s) 695
AsuHPI GGTGA 3 cut(s) 448, 806, 841
AvaI CYCGRG 1 cut(s) 410
AvaII GGWCC 3 cut(s) 598, 663, 913
BaeGI GKGCMC 2 cut(s) 733, 906
BaeI ACNNNNGTAYC 2 cut(s) 111, 144
BamHI GGATCC 2 cut(s) 33, 554
BanII GRGCYC 1 cut(s) 733
BauI CACGAG 1 cut(s) 764
BbrPI CACGTG 1 cut(s) 907
BbsI GAAGAC 1 cut(s) 194
Bbv12I GWGCWC 2 cut(s) 417, 906
BbvI GCAGC 3 cut(s) 293, 335, 687
BccI CCATC 5 cut(s) 51, 377, 461, 787, 791
BceAI ACGGC 6 cut(s) 428, 688, 802, 805, 856, 1036
BciT130I CCWGG 3 cut(s) 460, 473, 638
BcnI CCSGG 1 cut(s) 695
BcoDI GTCTC 4 cut(s) 162, 279, 301, 407
BfaI CTAG 2 cut(s) 380, 546
BglI GCCNNNNNGGC 1 cut(s) 449
BisI GCNGC 8 cut(s) 307, 324, 349, 429, 701, 844, 879, 1024
BlsI GCNGC 8 cut(s) 308, 325, 350, 430, 702, 845, 880, 1025
Bme1390I CCNGG 4 cut(s) 460, 473, 638, 695
Bme18I GGWCC 3 cut(s) 598, 663, 913
BmeT110I CYCGRG 1 cut(s) 410
BmgT120I GGNCC 5 cut(s) 598, 663, 729, 730, 913
BmiI GGNNCC 3 cut(s) 35, 556, 731
BmrFI CCNGG 4 cut(s) 460, 473, 638, 695
BmrI ACTGGG 2 cut(s) 258, 280
BmsI GCATC 1 cut(s) 1018
BmuI ACTGGG 2 cut(s) 258, 280
BpiI GAAGAC 1 cut(s) 194
Bpu10I CCTNAGC 1 cut(s) 734
BpuMI CCSGG 1 cut(s) 695
BsaAI YACGTR 1 cut(s) 907
BsaBI GATNNNNATC 1 cut(s) 614
BsaHI GRCGYC 1 cut(s) 441
BsaI GGTCTC 2 cut(s) 279, 301
BsaJI CCNNGG 3 cut(s) 619, 818, 969
BsaWI WCCGGW 2 cut(s) 36, 915
BsaXI ACNNNNNCTCC 4 cut(s) 57, 87, 461, 491
Bsc4I CCNNNNNNNGG 7 cut(s) 270, 292, 459, 678, 745, 922, 964
Bse118I RCCGGY 1 cut(s) 915
Bse1I ACTGG 4 cut(s) 202, 264, 286, 316
Bse8I GATNNNNATC 1 cut(s) 614
BseBI CCWGG 3 cut(s) 460, 473, 638
BseDI CCNNGG 3 cut(s) 619, 818, 969
BseGI GGATG 1 cut(s) 369
BseJI GATNNNNATC 1 cut(s) 614
BseLI CCNNNNNNNGG 7 cut(s) 270, 292, 459, 678, 745, 922, 964
BseMII CTCAG 2 cut(s) 344, 725
BseNI ACTGG 4 cut(s) 202, 264, 286, 316
BseRI GAGGAG 2 cut(s) 469, 938
BseSI GKGCMC 2 cut(s) 733, 906
BseXI GCAGC 3 cut(s) 293, 335, 687
BseYI CCCAGC 1 cut(s) 373
Bsh1236I CGCG 5 cut(s) 551, 575, 585, 878, 897
Bsh1285I CGRYCG 1 cut(s) 694
BshFI GGCC 4 cut(s) 452, 476, 731, 968
BshTI ACCGGT 1 cut(s) 915
BsiEI CGRYCG 1 cut(s) 694
BsiHKAI GWGCWC 2 cut(s) 417, 906
BsiHKCI CYCGRG 1 cut(s) 410
BsiSI CCGG 4 cut(s) 37, 694, 743, 916
BslI CCNNNNNNNGG 7 cut(s) 270, 292, 459, 678, 745, 922, 964
BsmAI GTCTC 4 cut(s) 162, 279, 301, 407
BsmBI CGTCTC 2 cut(s) 162, 407
BsnI GGCC 4 cut(s) 452, 476, 731, 968
Bso31I GGTCTC 2 cut(s) 279, 301
BsoBI CYCGRG 1 cut(s) 410
Bsp120I GGGCCC 1 cut(s) 729
Bsp1286I GDGCHC 3 cut(s) 417, 733, 906
BspANI GGCC 4 cut(s) 452, 476, 731, 968
BspCNI CTCAG 2 cut(s) 343, 726
BspFNI CGCG 5 cut(s) 551, 575, 585, 878, 897
BspLI GGNNCC 3 cut(s) 35, 556, 731
BspPI GGATC 6 cut(s) 28, 41, 549, 562, 812, 842
BspTNI GGTCTC 2 cut(s) 279, 301
BsrBI CCGCTC 1 cut(s) 834
BsrFI RCCGGY 1 cut(s) 915
BsrI ACTGG 4 cut(s) 202, 264, 286, 316
BssAI RCCGGY 1 cut(s) 915
BssECI CCNNGG 3 cut(s) 619, 818, 969
BssNI GRCGYC 1 cut(s) 441
BssSI CACGAG 1 cut(s) 764
Bst2BI CACGAG 1 cut(s) 764
Bst2UI CCWGG 3 cut(s) 460, 473, 638
Bst4CI ACNGT 3 cut(s) 401, 813, 885
Bst6I CTCTTC 2 cut(s) 165, 193
BstACI GRCGYC 1 cut(s) 441
BstBAI YACGTR 1 cut(s) 907
BstC8I GCNNGC 2 cut(s) 242, 740
BstDEI CTNAG 2 cut(s) 330, 734
BstDSI CCRYGG 1 cut(s) 818
BstENI CCTNNNNNAGG 1 cut(s) 743
BstF5I GGATG 1 cut(s) 369
BstFNI CGCG 5 cut(s) 551, 575, 585, 878, 897
BstHHI GCGC 2 cut(s) 551, 587
BstMAI GTCTC 4 cut(s) 162, 279, 301, 407
BstMCI CGRYCG 1 cut(s) 694
BstMWI GCNNNNNNNGC 6 cut(s) 354, 449, 482, 572, 631, 840
BstNI CCWGG 3 cut(s) 460, 473, 638
BstSCI CCNGG 4 cut(s) 458, 471, 636, 693
BstSLI GKGCMC 2 cut(s) 733, 906
BstUI CGCG 5 cut(s) 551, 575, 585, 878, 897
BstV1I GCAGC 3 cut(s) 293, 335, 687
BstV2I GAAGAC 1 cut(s) 194
BstX2I RGATCY 2 cut(s) 33, 554
BstYI RGATCY 2 cut(s) 33, 554
BsuRI GGCC 4 cut(s) 452, 476, 731, 968
BtgI CCRYGG 1 cut(s) 818
BtgZI GCGATG 1 cut(s) 1041
BtsCI GGATG 1 cut(s) 369
BtsI GCAGTG 1 cut(s) 343
BtsIMutI CAGTG 5 cut(s) 271, 293, 323, 343, 881
Cac8I GCNNGC 2 cut(s) 242, 740
CfoI GCGC 2 cut(s) 551, 587
Cfr10I RCCGGY 1 cut(s) 915
Cfr13I GGNCC 5 cut(s) 598, 663, 729, 730, 913
CpoI CGGWCCG 1 cut(s) 913
CseI GACGC 3 cut(s) 233, 298, 449
Csp6I GTAC 2 cut(s) 397, 838
CspAI ACCGGT 1 cut(s) 915
CspCI CAANNNNNGTGG 4 cut(s) 82, 117, 752, 787
CspI CGGWCCG 1 cut(s) 913
CviAII CATG 2 cut(s) 538, 938
CviQI GTAC 2 cut(s) 397, 838
DdeI CTNAG 2 cut(s) 330, 734
EaeI YGGCCR 1 cut(s) 450
Eam1104I CTCTTC 2 cut(s) 165, 193
EarI CTCTTC 2 cut(s) 165, 193
Eco147I AGGCCT 1 cut(s) 476
Eco24I GRGCYC 1 cut(s) 733
Eco31I GGTCTC 2 cut(s) 279, 301
Eco47I GGWCC 3 cut(s) 598, 663, 913
Eco72I CACGTG 1 cut(s) 907
Eco88I CYCGRG 1 cut(s) 410
EcoNI CCTNNNNNAGG 1 cut(s) 743
EcoO109I RGGNCCY 1 cut(s) 730
EcoRII CCWGG 3 cut(s) 458, 471, 636
EcoT38I GRGCYC 1 cut(s) 733
Esp3I CGTCTC 2 cut(s) 162, 407
FaeI CATG 2 cut(s) 541, 941
FaiI YATR 6 cut(s) 539, 596, 939, 981, 1055, 1057
FatI CATG 2 cut(s) 537, 937
FauI CCCGC 6 cut(s) 433, 544, 568, 644, 827, 890
FauNDI CATATG 1 cut(s) 1055
FblI GTMKAC 1 cut(s) 580
Fnu4HI GCNGC 8 cut(s) 307, 324, 349, 429, 701, 844, 879, 1024
FokI GGATG 1 cut(s) 356
FriOI GRGCYC 1 cut(s) 733
Fsp4HI GCNGC 8 cut(s) 307, 324, 349, 429, 701, 844, 879, 1024
FspBI CTAG 2 cut(s) 380, 546
GlaI GCGC 2 cut(s) 550, 586
GluI GCNGC 8 cut(s) 307, 324, 349, 429, 701, 844, 879, 1024
GsaI CCCAGC 1 cut(s) 377
HaeIII GGCC 4 cut(s) 452, 476, 731, 968
HapII CCGG 4 cut(s) 37, 694, 743, 916
HgaI GACGC 3 cut(s) 233, 298, 449
HhaI GCGC 2 cut(s) 551, 587
Hin1I GRCGYC 1 cut(s) 441
Hin1II CATG 2 cut(s) 541, 941
Hin6I GCGC 2 cut(s) 549, 585
HinP1I GCGC 2 cut(s) 549, 585
HincII GTYRAC 2 cut(s) 658, 688
HindII GTYRAC 2 cut(s) 658, 688
HinfI GANTC 3 cut(s) 494, 668, 890
HpaII CCGG 4 cut(s) 37, 694, 743, 916
HphI GGTGA 3 cut(s) 448, 806, 841
Hpy166II GTNNAC 5 cut(s) 76, 581, 658, 688, 904
Hpy188I TCNGA 5 cut(s) 249, 592, 620, 673, 809
Hpy188III TCNNGA 1 cut(s) 758
Hpy8I GTNNAC 5 cut(s) 76, 581, 658, 688, 904
Hpy99I CGWCG 5 cut(s) 314, 395, 440, 443, 491
HpyAV CCTTC 2 cut(s) 795, 862
HpyCH4III ACNGT 3 cut(s) 401, 813, 885
HpyCH4IV ACGT 1 cut(s) 906
HpyCH4V TGCA 3 cut(s) 634, 904, 1043
HpyF10VI GCNNNNNNNGC 6 cut(s) 354, 449, 482, 572, 631, 840
HpyF3I CTNAG 2 cut(s) 330, 734
HpySE526I ACGT 1 cut(s) 906
Hsp92I GRCGYC 1 cut(s) 441
Hsp92II CATG 2 cut(s) 541, 941
HspAI GCGC 2 cut(s) 549, 585
LmnI GCTCC 1 cut(s) 946
Lsp1109I GCAGC 3 cut(s) 293, 335, 687
LweI GCATC 1 cut(s) 1018
MaeI CTAG 2 cut(s) 380, 546
MaeII ACGT 1 cut(s) 906
MaeIII GTNAC 5 cut(s) 143, 211, 758, 891, 907
MbiI CCGCTC 1 cut(s) 834
MboII GAAGA 6 cut(s) 144, 152, 166, 180, 194, 227
MflI RGATCY 2 cut(s) 33, 554
MhlI GDGCHC 3 cut(s) 417, 733, 906
MluCI AATT 4 cut(s) 13, 107, 113, 995
MlyI GAGTC 1 cut(s) 899
MmeI TCCRAC 2 cut(s) 43, 867
MseI TTAA 1 cut(s) 990
MspA1I CMGCKG 1 cut(s) 853
MspI CCGG 4 cut(s) 37, 694, 743, 916
MspR9I CCNGG 4 cut(s) 460, 473, 638, 695
MvaI CCWGG 3 cut(s) 460, 473, 638
MvnI CGCG 5 cut(s) 551, 575, 585, 878, 897
MwoI GCNNNNNNNGC 6 cut(s) 354, 449, 482, 572, 631, 840
NciI CCSGG 1 cut(s) 695
NdeI CATATG 1 cut(s) 1055
NlaIII CATG 2 cut(s) 541, 941
NlaIV GGNNCC 3 cut(s) 35, 556, 731
NmeAIII GCCGAG 1 cut(s) 994
NmuCI GTSAC 5 cut(s) 143, 211, 758, 891, 907
PceI AGGCCT 1 cut(s) 476
PcsI WCGNNNNNNNCGW 1 cut(s) 441
PfeI GAWTC 2 cut(s) 494, 668
PflMI CCANNNNNTGG 1 cut(s) 459
PinAI ACCGGT 1 cut(s) 915
PkrI GCNGC 8 cut(s) 308, 325, 350, 430, 702, 845, 880, 1025
PleI GAGTC 1 cut(s) 898
PmaCI CACGTG 1 cut(s) 907
PmlI CACGTG 1 cut(s) 907
PpsI GAGTC 1 cut(s) 898
Ppu21I YACGTR 1 cut(s) 907
Psp6I CCWGG 3 cut(s) 458, 471, 636
PspCI CACGTG 1 cut(s) 907
PspFI CCCAGC 1 cut(s) 373
PspGI CCWGG 3 cut(s) 458, 471, 636
PspN4I GGNNCC 3 cut(s) 35, 556, 731
PspOMI GGGCCC 1 cut(s) 729
PspPI GGNCC 5 cut(s) 598, 663, 729, 730, 913
PsuI RGATCY 2 cut(s) 33, 554
RsaI GTAC 2 cut(s) 398, 839
RsaNI GTAC 2 cut(s) 397, 838
Rsr2I CGGWCCG 1 cut(s) 913
RsrII CGGWCCG 1 cut(s) 913
SaqAI TTAA 1 cut(s) 990
SatI GCNGC 8 cut(s) 307, 324, 349, 429, 701, 844, 879, 1024
Sau96I GGNCC 5 cut(s) 598, 663, 729, 730, 913
SchI GAGTC 1 cut(s) 899
ScrFI CCNGG 4 cut(s) 460, 473, 638, 695
SduI GDGCHC 3 cut(s) 417, 733, 906
SetI ASST 8 cut(s) 220, 255, 308, 461, 603, 683, 909, 951
SfaNI GCATC 1 cut(s) 1018
SinI GGWCC 3 cut(s) 598, 663, 913
Sse9I AATT 4 cut(s) 13, 107, 113, 995
SseBI AGGCCT 1 cut(s) 476
SspMI CTAG 2 cut(s) 380, 546
StuI AGGCCT 1 cut(s) 476
StyD4I CCNGG 4 cut(s) 458, 471, 636, 693
TaaI ACNGT 3 cut(s) 401, 813, 885
TaiI ACGT 1 cut(s) 909
TaqI TCGA 2 cut(s) 393, 1049
TaqII GACCGA 4 cut(s) 162, 261, 283, 680
TasI AATT 4 cut(s) 13, 107, 113, 995
TauI GCSGC 5 cut(s) 326, 431, 846, 881, 1026
TfiI GAWTC 2 cut(s) 494, 668
Tru1I TTAA 1 cut(s) 990
Tru9I TTAA 1 cut(s) 990
TscAI CASTG 5 cut(s) 271, 293, 323, 350, 888
TseFI GTSAC 5 cut(s) 143, 211, 758, 891, 907
TseI GCWGC 3 cut(s) 306, 348, 700
Tsp45I GTSAC 5 cut(s) 143, 211, 758, 891, 907
TspDTI ATGAA 3 cut(s) 84, 523, 926
TspGWI ACGGA 6 cut(s) 46, 289, 379, 502, 504, 926
TspRI CASTG 5 cut(s) 271, 293, 323, 350, 888
Van91I CCANNNNNTGG 1 cut(s) 459
VneI GTGCAC 1 cut(s) 902
VpaK11BI GGWCC 3 cut(s) 598, 663, 913
XagI CCTNNNNNAGG 1 cut(s) 743
XapI RAATTY 3 cut(s) 107, 113, 995
XcmI CCANNNNNNNNNTGG 2 cut(s) 263, 285
XmiI GTMKAC 1 cut(s) 580
XspI CTAG 2 cut(s) 380, 546
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.