Rmu_sc0005320.1_g000006

carboxylesterase 8

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0005320.1
OG0005485
Physical Location & Seq
Reverse (-)
22315 .. 23349
1035 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0005320.1_g000006.1.cds

Sequence Viewer

Length: 1035 bp
atggcagatcaacaatcctcaatcgacccctacaagttcctcaaaatcgttcccaacccagatggctcactcacccgcctcgccccactctcaatcgcacccccaaccgagtcaatccccgccgattctgatcccaactcatcccaactcgtcctctccaaggacatccctctcgacccaaaatcccaaacctcccttcgcctctttaaacccctcaatctccccccaaacccaaacctaaaccctaacctccctctcctcatctacatccacggcggcggcttcatcatctggagcgccgcctctcagcctttccaccactgctgctctcgcctcgctctctctatcccagccctagtcctctccgtcgagtaccgtctcgcccccgagcaccgcctccccgccgcctacgacgacgccgtttcggccatcacctggctcaaaaaccaggcctcctctgccgacggagattcgtggctgaaaaactccgttgatttcgccaagtgtttcatcatgggaactagcgcgggatccaatatcgcctaccacgcgggtctacgcgcttccgatatggacctctccccgatcaaaatccgagggctgatattgcaccaggcgtatttcggcggggtcaacaggaccgaatccgagatcaggttagtcaacgaccgggtgctgccgttagttgctaacgacttgatgtgggccctgagcctgccggagggggcagatcgtgaccacgagtattgtaatccatctatcaacggcggtgatggaaggatcggacggttgccgtggtgtttggtgagcgggaatggcggggacccgctggtggatagggagaaggagatcgcggcactgttggagtcacgcggggtgcaagtgacggcccggtttgaggagggtggttttcatggtgcggagctatttgaccgcaacaaggccgaggctttctatgagattgttaaggaatttatcggctcttgttgcgcacgcggtagcgatgatgttgccgtggtaaaatcagccatgtga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0009056 GO:0016787
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

344

Amino Acids

37.33

Weight (kDa)

5.4

Isoelectric Point (pI)

34.87

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 991
AccB7I CCANNNNNTGG 1 cut(s) 435
AccBSI CCGCTC 1 cut(s) 810
AccI GTMKAC 1 cut(s) 556
AccII CGCG 6 cut(s) 527, 551, 561, 854, 873, 996
AclWI GGATC 4 cut(s) 125, 525, 538, 788
AcoI YGGCCR 1 cut(s) 426
AcsI RAATTY 1 cut(s) 971
AcyI GRCGYC 1 cut(s) 417
AfaI GTAC 1 cut(s) 374
AfiI CCNNNNNNNGG 7 cut(s) 160, 435, 654, 721, 832, 898, 940
AjnI CCWGG 3 cut(s) 434, 447, 612
AjuI GAANNNNNNNTTGG 2 cut(s) 180, 212
AluBI AGCT 1 cut(s) 925
AluI AGCT 1 cut(s) 925
Alw21I GWGCWC 1 cut(s) 393
Alw26I GTCTC 1 cut(s) 383
AlwI GGATC 4 cut(s) 125, 525, 538, 788
Ama87I CYCGRG 1 cut(s) 386
AoxI GGCC 5 cut(s) 426, 450, 705, 888, 942
ApaI GGGCCC 1 cut(s) 709
ApeKI GCWGC 2 cut(s) 324, 676
ApoI RAATTY 1 cut(s) 971
AspLEI GCGC 4 cut(s) 299, 527, 563, 992
AspS9I GGNCC 6 cut(s) 574, 639, 705, 706, 823, 889
AsuC2I CCSGG 2 cut(s) 671, 892
AsuHPI GGTGA 4 cut(s) 64, 424, 782, 817
AvaI CYCGRG 1 cut(s) 386
AvaII GGWCC 3 cut(s) 574, 639, 823
BaeGI GKGCMC 1 cut(s) 709
BamHI GGATCC 1 cut(s) 530
BanII GRGCYC 1 cut(s) 709
BauI CACGAG 1 cut(s) 740
Bbv12I GWGCWC 1 cut(s) 393
BbvI GCAGC 2 cut(s) 311, 663
BccI CCATC 4 cut(s) 56, 437, 763, 767
BceAI ACGGC 7 cut(s) 289, 404, 664, 778, 781, 903, 998
BcgI CGANNNNNNTGC 2 cut(s) 992, 1026
BciT130I CCWGG 3 cut(s) 436, 449, 614
BcnI CCSGG 2 cut(s) 671, 892
BcoDI GTCTC 1 cut(s) 383
BfaI CTAG 2 cut(s) 356, 522
BfoI RGCGCY 1 cut(s) 300
BglI GCCNNNNNGGC 1 cut(s) 425
BisI GCNGC 7 cut(s) 277, 280, 300, 325, 405, 677, 855
BlsI GCNGC 7 cut(s) 278, 281, 301, 326, 406, 678, 856
Bme1390I CCNGG 5 cut(s) 436, 449, 614, 671, 892
Bme18I GGWCC 3 cut(s) 574, 639, 823
BmeT110I CYCGRG 1 cut(s) 386
BmgT120I GGNCC 6 cut(s) 574, 639, 705, 706, 823, 889
BmiI GGNNCC 4 cut(s) 532, 707, 824, 825
BmrFI CCNGG 5 cut(s) 436, 449, 614, 671, 892
BpmI CTGGAG 1 cut(s) 313
Bpu10I CCTNAGC 1 cut(s) 710
BpuMI CCSGG 2 cut(s) 671, 892
BsaBI GATNNNNATC 2 cut(s) 129, 590
BsaHI GRCGYC 1 cut(s) 417
BsaJI CCNNGG 6 cut(s) 159, 271, 595, 794, 945, 1014
BsaXI ACNNNNNCTCC 2 cut(s) 437, 467
Bsc4I CCNNNNNNNGG 7 cut(s) 160, 435, 654, 721, 832, 898, 940
Bse8I GATNNNNATC 2 cut(s) 129, 590
BseBI CCWGG 3 cut(s) 436, 449, 614
BseDI CCNNGG 6 cut(s) 159, 271, 595, 794, 945, 1014
BseGI GGATG 3 cut(s) 140, 165, 267
BseJI GATNNNNATC 2 cut(s) 129, 590
BseLI CCNNNNNNNGG 7 cut(s) 160, 435, 654, 721, 832, 898, 940
BseMII CTCAG 2 cut(s) 320, 701
BseRI GAGGAG 3 cut(s) 248, 445, 914
BseSI GKGCMC 1 cut(s) 709
BseXI GCAGC 2 cut(s) 311, 663
BseYI CCCAGC 1 cut(s) 349
Bsh1236I CGCG 6 cut(s) 527, 551, 561, 854, 873, 996
Bsh1285I CGRYCG 1 cut(s) 670
BshFI GGCC 5 cut(s) 428, 452, 707, 890, 944
BsiEI CGRYCG 1 cut(s) 670
BsiHKAI GWGCWC 1 cut(s) 393
BsiHKCI CYCGRG 1 cut(s) 386
BsiSI CCGG 3 cut(s) 670, 719, 892
BslFI GGGAC 1 cut(s) 836
BslI CCNNNNNNNGG 7 cut(s) 160, 435, 654, 721, 832, 898, 940
BsmAI GTCTC 1 cut(s) 383
BsmBI CGTCTC 1 cut(s) 383
BsmFI GGGAC 1 cut(s) 836
BsnI GGCC 5 cut(s) 428, 452, 707, 890, 944
BsoBI CYCGRG 1 cut(s) 386
Bsp120I GGGCCC 1 cut(s) 705
Bsp1286I GDGCHC 2 cut(s) 393, 709
Bsp143I GATC 8 cut(s) 7, 130, 530, 585, 651, 730, 780, 849
BspANI GGCC 5 cut(s) 428, 452, 707, 890, 944
BspCNI CTCAG 2 cut(s) 319, 702
BspFNI CGCG 6 cut(s) 527, 551, 561, 854, 873, 996
BspLI GGNNCC 4 cut(s) 532, 707, 824, 825
BspPI GGATC 4 cut(s) 125, 525, 538, 788
BsrBI CCGCTC 1 cut(s) 810
BssECI CCNNGG 6 cut(s) 159, 271, 595, 794, 945, 1014
BssMI GATC 8 cut(s) 7, 130, 530, 585, 651, 730, 780, 849
BssNI GRCGYC 1 cut(s) 417
BssSI CACGAG 1 cut(s) 740
BssT1I CCWWGG 1 cut(s) 159
Bst2BI CACGAG 1 cut(s) 740
Bst2UI CCWGG 3 cut(s) 436, 449, 614
Bst4CI ACNGT 3 cut(s) 377, 789, 861
BstACI GRCGYC 1 cut(s) 417
BstC8I GCNNGC 2 cut(s) 716, 994
BstDEI CTNAG 2 cut(s) 306, 710
BstDSI CCRYGG 3 cut(s) 271, 794, 1014
BstENI CCTNNNNNAGG 2 cut(s) 158, 719
BstF5I GGATG 3 cut(s) 140, 165, 267
BstFNI CGCG 6 cut(s) 527, 551, 561, 854, 873, 996
BstH2I RGCGCY 1 cut(s) 300
BstHHI GCGC 4 cut(s) 299, 527, 563, 992
BstKTI GATC 8 cut(s) 10, 133, 533, 588, 654, 733, 783, 852
BstMAI GTCTC 1 cut(s) 383
BstMBI GATC 8 cut(s) 7, 130, 530, 585, 651, 730, 780, 849
BstMCI CGRYCG 1 cut(s) 670
BstMWI GCNNNNNNNGC 7 cut(s) 330, 425, 458, 548, 607, 816, 987
BstNI CCWGG 3 cut(s) 436, 449, 614
BstSCI CCNGG 5 cut(s) 434, 447, 612, 669, 890
BstSLI GKGCMC 1 cut(s) 709
BstUI CGCG 6 cut(s) 527, 551, 561, 854, 873, 996
BstV1I GCAGC 2 cut(s) 311, 663
BstX2I RGATCY 1 cut(s) 530
BstYI RGATCY 1 cut(s) 530
BsuRI GGCC 5 cut(s) 428, 452, 707, 890, 944
BtgI CCRYGG 3 cut(s) 271, 794, 1014
BtgZI GCGATG 1 cut(s) 1017
BtsCI GGATG 3 cut(s) 140, 165, 267
BtsI GCAGTG 1 cut(s) 319
BtsIMutI CAGTG 2 cut(s) 319, 857
Cac8I GCNNGC 2 cut(s) 716, 994
CfoI GCGC 4 cut(s) 299, 527, 563, 992
Cfr13I GGNCC 6 cut(s) 574, 639, 705, 706, 823, 889
CseI GACGC 1 cut(s) 425
Csp6I GTAC 1 cut(s) 373
CspCI CAANNNNNGTGG 2 cut(s) 728, 763
CviAII CATG 3 cut(s) 514, 914, 1030
CviQI GTAC 1 cut(s) 373
DdeI CTNAG 2 cut(s) 306, 710
DpnI GATC 8 cut(s) 9, 132, 532, 587, 653, 732, 782, 851
DpnII GATC 8 cut(s) 7, 130, 530, 585, 651, 730, 780, 849
DraI TTTAAA 1 cut(s) 208
EaeI YGGCCR 1 cut(s) 426
Eco130I CCWWGG 1 cut(s) 159
Eco147I AGGCCT 1 cut(s) 452
Eco24I GRGCYC 1 cut(s) 709
Eco47I GGWCC 3 cut(s) 574, 639, 823
Eco88I CYCGRG 1 cut(s) 386
EcoNI CCTNNNNNAGG 2 cut(s) 158, 719
EcoO109I RGGNCCY 2 cut(s) 706, 823
EcoRII CCWGG 3 cut(s) 434, 447, 612
EcoT14I CCWWGG 1 cut(s) 159
EcoT38I GRGCYC 1 cut(s) 709
ErhI CCWWGG 1 cut(s) 159
Esp3I CGTCTC 1 cut(s) 383
FaeI CATG 3 cut(s) 517, 917, 1033
FaiI YATR 5 cut(s) 515, 572, 915, 957, 1031
FaqI GGGAC 1 cut(s) 836
FatI CATG 3 cut(s) 513, 913, 1029
FblI GTMKAC 1 cut(s) 556
Fnu4HI GCNGC 7 cut(s) 277, 280, 300, 325, 405, 677, 855
FokI GGATG 3 cut(s) 127, 152, 254
FriOI GRGCYC 1 cut(s) 709
Fsp4HI GCNGC 7 cut(s) 277, 280, 300, 325, 405, 677, 855
FspBI CTAG 2 cut(s) 356, 522
FspI TGCGCA 1 cut(s) 991
GlaI GCGC 4 cut(s) 298, 526, 562, 991
GluI GCNGC 7 cut(s) 277, 280, 300, 325, 405, 677, 855
GsaI CCCAGC 1 cut(s) 353
GsuI CTGGAG 1 cut(s) 313
HaeII RGCGCY 1 cut(s) 300
HaeIII GGCC 5 cut(s) 428, 452, 707, 890, 944
HapII CCGG 3 cut(s) 670, 719, 892
HgaI GACGC 1 cut(s) 425
HhaI GCGC 4 cut(s) 299, 527, 563, 992
Hin1I GRCGYC 1 cut(s) 417
Hin1II CATG 3 cut(s) 517, 917, 1033
Hin6I GCGC 4 cut(s) 297, 525, 561, 990
HinP1I GCGC 4 cut(s) 297, 525, 561, 990
HincII GTYRAC 2 cut(s) 634, 664
HindII GTYRAC 2 cut(s) 634, 664
HinfI GANTC 5 cut(s) 110, 125, 470, 644, 866
HpaII CCGG 3 cut(s) 670, 719, 892
HphI GGTGA 4 cut(s) 64, 424, 782, 817
Hpy166II GTNNAC 3 cut(s) 557, 634, 664
Hpy188I TCNGA 5 cut(s) 130, 568, 596, 649, 785
Hpy188III TCNNGA 3 cut(s) 173, 292, 734
Hpy8I GTNNAC 3 cut(s) 557, 634, 664
Hpy99I CGWCG 4 cut(s) 371, 416, 419, 467
HpyAV CCTTC 3 cut(s) 206, 771, 838
HpyCH4III ACNGT 3 cut(s) 377, 789, 861
HpyCH4V TGCA 2 cut(s) 610, 880
HpyF10VI GCNNNNNNNGC 7 cut(s) 330, 425, 458, 548, 607, 816, 987
HpyF3I CTNAG 2 cut(s) 306, 710
Hsp92I GRCGYC 1 cut(s) 417
Hsp92II CATG 3 cut(s) 517, 917, 1033
HspAI GCGC 4 cut(s) 297, 525, 561, 990
KflI GGGWCCC 1 cut(s) 823
Kzo9I GATC 8 cut(s) 7, 130, 530, 585, 651, 730, 780, 849
LmnI GCTCC 2 cut(s) 294, 922
Lsp1109I GCAGC 2 cut(s) 311, 663
MaeI CTAG 2 cut(s) 356, 522
MaeIII GTNAC 3 cut(s) 734, 867, 883
MalI GATC 8 cut(s) 9, 132, 532, 587, 653, 732, 782, 851
MbiI CCGCTC 1 cut(s) 810
MboI GATC 8 cut(s) 7, 130, 530, 585, 651, 730, 780, 849
MflI RGATCY 1 cut(s) 530
MhlI GDGCHC 2 cut(s) 393, 709
MluCI AATT 1 cut(s) 971
MlyI GAGTC 2 cut(s) 119, 875
MmeI TCCRAC 1 cut(s) 843
MseI TTAA 2 cut(s) 207, 966
MspA1I CMGCKG 1 cut(s) 829
MspI CCGG 3 cut(s) 670, 719, 892
MspR9I CCNGG 5 cut(s) 436, 449, 614, 671, 892
MvaI CCWGG 3 cut(s) 436, 449, 614
MvnI CGCG 6 cut(s) 527, 551, 561, 854, 873, 996
MwoI GCNNNNNNNGC 7 cut(s) 330, 425, 458, 548, 607, 816, 987
NciI CCSGG 2 cut(s) 671, 892
NdeII GATC 8 cut(s) 7, 130, 530, 585, 651, 730, 780, 849
NlaIII CATG 3 cut(s) 517, 917, 1033
NlaIV GGNNCC 4 cut(s) 532, 707, 824, 825
NmeAIII GCCGAG 1 cut(s) 970
NmuCI GTSAC 3 cut(s) 734, 867, 883
NsbI TGCGCA 1 cut(s) 991
PceI AGGCCT 1 cut(s) 452
PcsI WCGNNNNNNNCGW 1 cut(s) 417
PfeI GAWTC 3 cut(s) 125, 470, 644
PflMI CCANNNNNTGG 1 cut(s) 435
PkrI GCNGC 7 cut(s) 278, 281, 301, 326, 406, 678, 856
PleI GAGTC 2 cut(s) 118, 874
PpsI GAGTC 2 cut(s) 118, 874
PpuMI RGGWCCY 1 cut(s) 823
Psp5II RGGWCCY 1 cut(s) 823
Psp6I CCWGG 3 cut(s) 434, 447, 612
PspFI CCCAGC 1 cut(s) 349
PspGI CCWGG 3 cut(s) 434, 447, 612
PspN4I GGNNCC 4 cut(s) 532, 707, 824, 825
PspOMI GGGCCC 1 cut(s) 705
PspPI GGNCC 6 cut(s) 574, 639, 705, 706, 823, 889
PspPPI RGGWCCY 1 cut(s) 823
PsuI RGATCY 1 cut(s) 530
RsaI GTAC 1 cut(s) 374
RsaNI GTAC 1 cut(s) 373
SaqAI TTAA 2 cut(s) 207, 966
SatI GCNGC 7 cut(s) 277, 280, 300, 325, 405, 677, 855
Sau3AI GATC 8 cut(s) 7, 130, 530, 585, 651, 730, 780, 849
Sau96I GGNCC 6 cut(s) 574, 639, 705, 706, 823, 889
SchI GAGTC 2 cut(s) 119, 875
ScrFI CCNGG 5 cut(s) 436, 449, 614, 671, 892
SduI GDGCHC 2 cut(s) 393, 709
SetI ASST 7 cut(s) 194, 240, 252, 437, 579, 659, 927
SinI GGWCC 3 cut(s) 574, 639, 823
Sse9I AATT 1 cut(s) 971
SseBI AGGCCT 1 cut(s) 452
SspMI CTAG 2 cut(s) 356, 522
StuI AGGCCT 1 cut(s) 452
StyD4I CCNGG 5 cut(s) 434, 447, 612, 669, 890
StyI CCWWGG 1 cut(s) 159
TaaI ACNGT 3 cut(s) 377, 789, 861
TaqI TCGA 3 cut(s) 24, 174, 369
TaqII GACCGA 1 cut(s) 656
TasI AATT 1 cut(s) 971
TauI GCSGC 5 cut(s) 279, 282, 302, 407, 857
TfiI GAWTC 3 cut(s) 125, 470, 644
Tru1I TTAA 2 cut(s) 207, 966
Tru9I TTAA 2 cut(s) 207, 966
TscAI CASTG 2 cut(s) 326, 864
TseFI GTSAC 3 cut(s) 734, 867, 883
TseI GCWGC 2 cut(s) 324, 676
Tsp45I GTSAC 3 cut(s) 734, 867, 883
TspDTI ATGAA 3 cut(s) 274, 499, 902
TspGWI ACGGA 3 cut(s) 355, 478, 480
TspRI CASTG 2 cut(s) 326, 864
Van91I CCANNNNNTGG 1 cut(s) 435
VpaK11BI GGWCC 3 cut(s) 574, 639, 823
XagI CCTNNNNNAGG 2 cut(s) 158, 719
XapI RAATTY 1 cut(s) 971
XmiI GTMKAC 1 cut(s) 556
XspI CTAG 2 cut(s) 356, 522
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.