RLG00000028409

carboxylesterase 8

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0005485
Physical Location & Seq
Reverse (-)
22478660 .. 22479682
1023 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000028409

Sequence Viewer

Length: 1023 bp
ATGGCAGATCAACAATCCTCAATCGACCCCCACAAGTTCCTCAAAATCGTTCCCAACCCAGATGGCTCACTCACCCGCCTCGCCCCACTCCCAATCGCACCCACAACCGAGTCAATCCCCGCTGATTCTCACCCCAGCTCACCCCAACTCGTCCTCTCTAAGGACATCCCTCTCGACCCAAAATCCCAAACCTTCCTCCGCCTCTTCAAACCCCACAATCTCCCCCCAAACCCTAACCTCCCTCTCCTTATCTACTTCCACGGCGGCGGCTTCGTTCTCTTCAGCGCCGCCTCTCAGCCTTTCCACAACTCCTGCTCTCGCCTCGCCCTCTCTATCCCAGCCCTAGTCCTCTCCGTCGAGTACCGTCTCGCCCCCGAGCACCGCCTCCCCGCCGCCTACGACGACGCCATTTCGGCCATCACCTGGCTCAAAAACCAGGCCTCCTCTGCCGACGGAGATTCGTGGCTGAGAAACTCCGTAGATTTCTCCAAGTGTATCCTGATGGGAAGTAGCGCCGGGGCTAATATCGCCTACCACGCGGCTCTACGCGCTTGCGATGTGGACCTCTCCCCGATCAAGATCGGAGGGCTGATATTGCACCAGGCGTATTTCGGTGGCGTCAACAGGACCGAATCCGAGATCAGGTTCGTCAACGACCGGGTCCTGCCGTTACCCGCTAACGACTTGATGTGGGCCCTGAGTCTGCCGGAGGGGGTTGATCGTGACCACGAGTATTGTAATCCGTTTATGAACGGCGGTGATGGAAGGATCGGACGGTTGCCGTGGTGTTTGGTGGCCGGGTACGGCGGGGACCCACTGGTGGATAGACAGAAGGAGATGGCGGCATTGTTGGAGTCACGCGGGGTGCACGTGATGGCCCGGTTTAAGGAGGGTGGTTTTCATGGCGTGGAGCTATTTGACCCGAGTAAGGCCGAAGCGTTGTATGAGATTGTCAAGGAATTTATCGGCTCTTGTTGCGCGCACGGTAGCGGTGATGTTGTCGTGGCAAAATCAACCATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0009056 GO:0016787
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

341

Amino Acids

36.89

Weight (kDa)

5.93

Isoelectric Point (pI)

44.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BD-FAE PF20434 78 - 184 3.9e-12 BD-FAE
Abhydrolase_3 PF07859 83 - 303 3.3e-54 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 423
AccII CGCG 4 cut(s) 539, 549, 861, 980
AclWI GGATC 1 cut(s) 776
AcoI YGGCCR 2 cut(s) 414, 795
AcsI RAATTY 1 cut(s) 959
AcuI CTGAAG 1 cut(s) 265
AcvI CACGTG 1 cut(s) 871
AcyI GRCGYC 2 cut(s) 405, 618
AfaI GTAC 2 cut(s) 362, 803
AfiI CCNNNNNNNGG 6 cut(s) 160, 423, 642, 820, 886, 928
AgsI TTSAA 1 cut(s) 208
AjnI CCWGG 3 cut(s) 422, 435, 600
AluBI AGCT 2 cut(s) 138, 913
AluI AGCT 2 cut(s) 138, 913
Alw21I GWGCWC 2 cut(s) 381, 870
Alw26I GTCTC 1 cut(s) 371
Alw44I GTGCAC 1 cut(s) 866
AlwI GGATC 1 cut(s) 776
Ama87I CYCGRG 2 cut(s) 374, 922
AoxI GGCC 6 cut(s) 414, 438, 693, 795, 876, 930
ApaI GGGCCC 1 cut(s) 697
ApaLI GTGCAC 1 cut(s) 866
ApoI RAATTY 1 cut(s) 959
AspLEI GCGC 5 cut(s) 287, 515, 551, 980, 982
AspS9I GGNCC 7 cut(s) 562, 627, 661, 693, 694, 811, 877
AsuC2I CCSGG 4 cut(s) 517, 659, 799, 880
AsuHPI GGTGA 6 cut(s) 64, 122, 132, 412, 770, 1004
AvaI CYCGRG 2 cut(s) 374, 922
AvaII GGWCC 4 cut(s) 562, 627, 661, 811
BaeGI GKGCMC 2 cut(s) 697, 870
BanII GRGCYC 1 cut(s) 697
BauI CACGAG 1 cut(s) 728
BbrPI CACGTG 1 cut(s) 871
Bbv12I GWGCWC 2 cut(s) 381, 870
BccI CCATC 6 cut(s) 56, 425, 496, 755, 832, 868
BceAI ACGGC 5 cut(s) 277, 652, 766, 769, 820
BciT130I CCWGG 3 cut(s) 424, 437, 602
BciVI GTATCC 1 cut(s) 506
BcnI CCSGG 4 cut(s) 517, 659, 799, 880
BcoDI GTCTC 1 cut(s) 371
BfaI CTAG 1 cut(s) 344
BfoI RGCGCY 2 cut(s) 288, 516
BfuI GTATCC 1 cut(s) 506
BglI GCCNNNNNGGC 1 cut(s) 413
BisI GCNGC 6 cut(s) 265, 268, 288, 393, 540, 843
BlsI GCNGC 6 cut(s) 266, 269, 289, 394, 541, 844
Bme1390I CCNGG 7 cut(s) 424, 437, 517, 602, 659, 799, 880
Bme18I GGWCC 4 cut(s) 562, 627, 661, 811
BmeT110I CYCGRG 2 cut(s) 374, 922
BmgT120I GGNCC 7 cut(s) 562, 627, 661, 693, 694, 811, 877
BmiI GGNNCC 4 cut(s) 662, 695, 812, 813
BmrFI CCNGG 7 cut(s) 424, 437, 517, 602, 659, 799, 880
BpuMI CCSGG 4 cut(s) 517, 659, 799, 880
BsaAI YACGTR 1 cut(s) 871
BsaBI GATNNNNATC 1 cut(s) 578
BsaHI GRCGYC 2 cut(s) 405, 618
BsaJI CCNNGG 3 cut(s) 259, 516, 782
BsaXI ACNNNNNCTCC 2 cut(s) 425, 455
Bsc4I CCNNNNNNNGG 6 cut(s) 160, 423, 642, 820, 886, 928
Bse1I ACTGG 1 cut(s) 822
Bse8I GATNNNNATC 1 cut(s) 578
BseBI CCWGG 3 cut(s) 424, 437, 602
BseDI CCNNGG 3 cut(s) 259, 516, 782
BseGI GGATG 1 cut(s) 165
BseJI GATNNNNATC 1 cut(s) 578
BseLI CCNNNNNNNGG 6 cut(s) 160, 423, 642, 820, 886, 928
BseMII CTCAG 3 cut(s) 308, 458, 689
BseNI ACTGG 1 cut(s) 822
BsePI GCGCGC 1 cut(s) 978
BseRI GAGGAG 1 cut(s) 433
BseSI GKGCMC 2 cut(s) 697, 870
BseYI CCCAGC 2 cut(s) 134, 337
Bsh1236I CGCG 4 cut(s) 539, 549, 861, 980
Bsh1285I CGRYCG 1 cut(s) 658
BshFI GGCC 6 cut(s) 416, 440, 695, 797, 878, 932
BsiEI CGRYCG 1 cut(s) 658
BsiHKAI GWGCWC 2 cut(s) 381, 870
BsiHKCI CYCGRG 2 cut(s) 374, 922
BsiSI CCGG 5 cut(s) 516, 658, 707, 798, 880
BslFI GGGAC 1 cut(s) 824
BslI CCNNNNNNNGG 6 cut(s) 160, 423, 642, 820, 886, 928
BsmAI GTCTC 1 cut(s) 371
BsmBI CGTCTC 1 cut(s) 371
BsmFI GGGAC 1 cut(s) 824
BsnI GGCC 6 cut(s) 416, 440, 695, 797, 878, 932
BsoBI CYCGRG 2 cut(s) 374, 922
Bsp120I GGGCCC 1 cut(s) 693
Bsp1286I GDGCHC 3 cut(s) 381, 697, 870
Bsp143I GATC 6 cut(s) 7, 573, 579, 639, 718, 768
BspANI GGCC 6 cut(s) 416, 440, 695, 797, 878, 932
BspCNI CTCAG 3 cut(s) 307, 459, 690
BspFNI CGCG 4 cut(s) 539, 549, 861, 980
BspLI GGNNCC 4 cut(s) 662, 695, 812, 813
BspPI GGATC 1 cut(s) 776
BsrI ACTGG 1 cut(s) 822
BssECI CCNNGG 3 cut(s) 259, 516, 782
BssHII GCGCGC 1 cut(s) 978
BssMI GATC 6 cut(s) 7, 573, 579, 639, 718, 768
BssNI GRCGYC 2 cut(s) 405, 618
BssSI CACGAG 1 cut(s) 728
Bst2BI CACGAG 1 cut(s) 728
Bst2UI CCWGG 3 cut(s) 424, 437, 602
Bst4CI ACNGT 3 cut(s) 365, 777, 986
Bst6I CTCTTC 2 cut(s) 209, 284
BstACI GRCGYC 2 cut(s) 405, 618
BstBAI YACGTR 1 cut(s) 871
BstC8I GCNNGC 2 cut(s) 553, 980
BstDEI CTNAG 4 cut(s) 159, 294, 467, 698
BstDSI CCRYGG 2 cut(s) 259, 782
BstENI CCTNNNNNAGG 1 cut(s) 158
BstF5I GGATG 1 cut(s) 165
BstFNI CGCG 4 cut(s) 539, 549, 861, 980
BstH2I RGCGCY 2 cut(s) 288, 516
BstHHI GCGC 5 cut(s) 287, 515, 551, 980, 982
BstKTI GATC 6 cut(s) 10, 576, 582, 642, 721, 771
BstMAI GTCTC 1 cut(s) 371
BstMBI GATC 6 cut(s) 7, 573, 579, 639, 718, 768
BstMCI CGRYCG 1 cut(s) 658
BstMWI GCNNNNNNNGC 7 cut(s) 413, 446, 527, 536, 548, 595, 975
BstNI CCWGG 3 cut(s) 424, 437, 602
BstSCI CCNGG 7 cut(s) 422, 435, 515, 600, 657, 797, 878
BstSLI GKGCMC 2 cut(s) 697, 870
BstUI CGCG 4 cut(s) 539, 549, 861, 980
BsuI GTATCC 1 cut(s) 506
BsuRI GGCC 6 cut(s) 416, 440, 695, 797, 878, 932
BtgI CCRYGG 2 cut(s) 259, 782
BtgZI GCGATG 1 cut(s) 570
BtsCI GGATG 1 cut(s) 165
BtsIMutI CAGTG 1 cut(s) 815
Cac8I GCNNGC 2 cut(s) 553, 980
CfoI GCGC 5 cut(s) 287, 515, 551, 980, 982
Cfr13I GGNCC 7 cut(s) 562, 627, 661, 693, 694, 811, 877
CseI GACGC 2 cut(s) 413, 607
Csp6I GTAC 2 cut(s) 361, 802
CspCI CAANNNNNGTGG 2 cut(s) 716, 751
CviAII CATG 2 cut(s) 902, 1018
CviQI GTAC 2 cut(s) 361, 802
DdeI CTNAG 4 cut(s) 159, 294, 467, 698
DpnI GATC 6 cut(s) 9, 575, 581, 641, 720, 770
DpnII GATC 6 cut(s) 7, 573, 579, 639, 718, 768
EaeI YGGCCR 2 cut(s) 414, 795
Eam1104I CTCTTC 2 cut(s) 209, 284
EarI CTCTTC 2 cut(s) 209, 284
EciI GGCGGA 1 cut(s) 188
Eco147I AGGCCT 1 cut(s) 440
Eco24I GRGCYC 1 cut(s) 697
Eco47I GGWCC 4 cut(s) 562, 627, 661, 811
Eco57I CTGAAG 1 cut(s) 265
Eco72I CACGTG 1 cut(s) 871
Eco88I CYCGRG 2 cut(s) 374, 922
EcoNI CCTNNNNNAGG 1 cut(s) 158
EcoO109I RGGNCCY 3 cut(s) 661, 694, 811
EcoRII CCWGG 3 cut(s) 422, 435, 600
EcoT38I GRGCYC 1 cut(s) 697
Esp3I CGTCTC 1 cut(s) 371
FaeI CATG 2 cut(s) 905, 1021
FaiI YATR 4 cut(s) 749, 903, 945, 1019
FaqI GGGAC 1 cut(s) 824
FatI CATG 2 cut(s) 901, 1017
FauI CCCGC 6 cut(s) 83, 127, 397, 682, 800, 854
Fnu4HI GCNGC 6 cut(s) 265, 268, 288, 393, 540, 843
FokI GGATG 1 cut(s) 152
FriOI GRGCYC 1 cut(s) 697
Fsp4HI GCNGC 6 cut(s) 265, 268, 288, 393, 540, 843
FspBI CTAG 1 cut(s) 344
GlaI GCGC 5 cut(s) 286, 514, 550, 979, 981
GluI GCNGC 6 cut(s) 265, 268, 288, 393, 540, 843
GsaI CCCAGC 2 cut(s) 138, 341
HaeII RGCGCY 2 cut(s) 288, 516
HaeIII GGCC 6 cut(s) 416, 440, 695, 797, 878, 932
HapII CCGG 5 cut(s) 516, 658, 707, 798, 880
HgaI GACGC 2 cut(s) 413, 607
HhaI GCGC 5 cut(s) 287, 515, 551, 980, 982
Hin1I GRCGYC 2 cut(s) 405, 618
Hin1II CATG 2 cut(s) 905, 1021
Hin6I GCGC 5 cut(s) 285, 513, 549, 978, 980
HinP1I GCGC 5 cut(s) 285, 513, 549, 978, 980
HincII GTYRAC 2 cut(s) 622, 652
HindII GTYRAC 2 cut(s) 622, 652
HinfI GANTC 6 cut(s) 110, 125, 458, 632, 700, 854
HpaII CCGG 5 cut(s) 516, 658, 707, 798, 880
HphI GGTGA 6 cut(s) 64, 122, 132, 412, 770, 1004
Hpy166II GTNNAC 4 cut(s) 562, 622, 652, 868
Hpy188I TCNGA 3 cut(s) 584, 637, 773
Hpy188III TCNNGA 4 cut(s) 173, 499, 577, 722
Hpy8I GTNNAC 4 cut(s) 562, 622, 652, 868
Hpy99I CGWCG 4 cut(s) 359, 404, 407, 455
HpyAV CCTTC 3 cut(s) 202, 759, 826
HpyCH4III ACNGT 3 cut(s) 365, 777, 986
HpyCH4IV ACGT 1 cut(s) 870
HpyCH4V TGCA 2 cut(s) 598, 868
HpyF10VI GCNNNNNNNGC 7 cut(s) 413, 446, 527, 536, 548, 595, 975
HpyF3I CTNAG 4 cut(s) 159, 294, 467, 698
HpySE526I ACGT 1 cut(s) 870
Hsp92I GRCGYC 2 cut(s) 405, 618
Hsp92II CATG 2 cut(s) 905, 1021
HspAI GCGC 5 cut(s) 285, 513, 549, 978, 980
KflI GGGWCCC 1 cut(s) 811
Kzo9I GATC 6 cut(s) 7, 573, 579, 639, 718, 768
LmnI GCTCC 1 cut(s) 910
MaeI CTAG 1 cut(s) 344
MaeII ACGT 1 cut(s) 870
MaeIII GTNAC 3 cut(s) 669, 722, 855
MalI GATC 6 cut(s) 9, 575, 581, 641, 720, 770
MboI GATC 6 cut(s) 7, 573, 579, 639, 718, 768
MboII GAAGA 2 cut(s) 196, 271
MhlI GDGCHC 3 cut(s) 381, 697, 870
MluCI AATT 1 cut(s) 959
MlyI GAGTC 3 cut(s) 119, 709, 863
MmeI TCCRAC 1 cut(s) 831
MseI TTAA 1 cut(s) 885
MspA1I CMGCKG 1 cut(s) 122
MspI CCGG 5 cut(s) 516, 658, 707, 798, 880
MspR9I CCNGG 7 cut(s) 424, 437, 517, 602, 659, 799, 880
MvaI CCWGG 3 cut(s) 424, 437, 602
MvnI CGCG 4 cut(s) 539, 549, 861, 980
MwoI GCNNNNNNNGC 7 cut(s) 413, 446, 527, 536, 548, 595, 975
NciI CCSGG 4 cut(s) 517, 659, 799, 880
NdeII GATC 6 cut(s) 7, 573, 579, 639, 718, 768
NlaIII CATG 2 cut(s) 905, 1021
NlaIV GGNNCC 4 cut(s) 662, 695, 812, 813
NmuCI GTSAC 2 cut(s) 722, 855
PauI GCGCGC 1 cut(s) 978
PceI AGGCCT 1 cut(s) 440
PfeI GAWTC 3 cut(s) 125, 458, 632
PflFI GACNNNGTC 1 cut(s) 659
PflMI CCANNNNNTGG 1 cut(s) 423
PkrI GCNGC 6 cut(s) 266, 269, 289, 394, 541, 844
PleI GAGTC 3 cut(s) 118, 708, 862
PmaCI CACGTG 1 cut(s) 871
PmlI CACGTG 1 cut(s) 871
PpsI GAGTC 3 cut(s) 118, 708, 862
Ppu21I YACGTR 1 cut(s) 871
PpuMI RGGWCCY 2 cut(s) 661, 811
Psp5II RGGWCCY 2 cut(s) 661, 811
Psp6I CCWGG 3 cut(s) 422, 435, 600
PspCI CACGTG 1 cut(s) 871
PspFI CCCAGC 2 cut(s) 134, 337
PspGI CCWGG 3 cut(s) 422, 435, 600
PspN4I GGNNCC 4 cut(s) 662, 695, 812, 813
PspOMI GGGCCC 1 cut(s) 693
PspPI GGNCC 7 cut(s) 562, 627, 661, 693, 694, 811, 877
PspPPI RGGWCCY 2 cut(s) 661, 811
PsyI GACNNNGTC 1 cut(s) 659
PteI GCGCGC 1 cut(s) 978
RsaI GTAC 2 cut(s) 362, 803
RsaNI GTAC 2 cut(s) 361, 802
SaqAI TTAA 1 cut(s) 885
SatI GCNGC 6 cut(s) 265, 268, 288, 393, 540, 843
Sau3AI GATC 6 cut(s) 7, 573, 579, 639, 718, 768
Sau96I GGNCC 7 cut(s) 562, 627, 661, 693, 694, 811, 877
SchI GAGTC 3 cut(s) 119, 709, 863
ScrFI CCNGG 7 cut(s) 424, 437, 517, 602, 659, 799, 880
SduI GDGCHC 3 cut(s) 381, 697, 870
SetI ASST 8 cut(s) 140, 194, 240, 425, 567, 647, 873, 915
SinI GGWCC 4 cut(s) 562, 627, 661, 811
Sse9I AATT 1 cut(s) 959
SseBI AGGCCT 1 cut(s) 440
SspMI CTAG 1 cut(s) 344
StuI AGGCCT 1 cut(s) 440
StyD4I CCNGG 7 cut(s) 422, 435, 515, 600, 657, 797, 878
TaaI ACNGT 3 cut(s) 365, 777, 986
TaiI ACGT 1 cut(s) 873
TaqI TCGA 3 cut(s) 24, 174, 357
TaqII GACCGA 1 cut(s) 644
TasI AATT 1 cut(s) 959
TauI GCSGC 6 cut(s) 267, 270, 290, 395, 542, 845
TfiI GAWTC 3 cut(s) 125, 458, 632
Tru1I TTAA 1 cut(s) 885
Tru9I TTAA 1 cut(s) 885
TscAI CASTG 1 cut(s) 822
TseFI GTSAC 2 cut(s) 722, 855
Tsp45I GTSAC 2 cut(s) 722, 855
TspDTI ATGAA 2 cut(s) 764, 890
TspGWI ACGGA 4 cut(s) 343, 466, 468, 732
TspRI CASTG 1 cut(s) 822
Tth111I GACNNNGTC 1 cut(s) 659
Van91I CCANNNNNTGG 1 cut(s) 423
VneI GTGCAC 1 cut(s) 866
VpaK11BI GGWCC 4 cut(s) 562, 627, 661, 811
XagI CCTNNNNNAGG 1 cut(s) 158
XapI RAATTY 1 cut(s) 959
XspI CTAG 1 cut(s) 344
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.