Rh1DG229800

carboxylesterase 8

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0005485
Physical Location & Seq
Forward (+)
44191244 .. 44193636
2393 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG229800.1

Sequence Viewer

Length: 1011 bp
ATGGCAGATGAACAATCCTCAATCGACCCCTACAAGTTCCTCAAAATCGTTCCCAACCCAGATGGTTCACTCACCCGCCTCGCCCCAATACCAATCGCACCCACAACCGAGTCAATCCCCGCCGATTCTGATCCCAACTCATCCCAACTCGTCCTCTCCAAGGACATCCCTCTCGACCCAAAATCCAAAACCTCCCTCCGCCTCTTCAAACCCCACAATCTCCCCCCAAACCCTAACCTCCCTCTCCTCATCTACATCCACGGCGGCGGCTTCATCATCTGCAGCGCCGCCTCTCAGCCTTTCCACCAATGCTGCTCTCGCCTCGCCCTCTCCATCCCAGCCCTAGTCCTCTCCGTCGAGTACCGTCTCGCCCCCGAGCACCGCCTCCCCGCCGCCTACGACGACGCCGTTTCGGCCATCACCTGGCTCAAAAACCAGGCCTCCTCTGCCGACGGAGATTCGTGGCTGAAAAACTCCGTTGATTTCTCCAAGTGTTTCATCATGGGAACTAGCGCGGGATCCAATATCGCCTACCACGCGGGTCTACGCGCTTCCGATATGGACCTCTCCCCGATCAAAATCCGAGGGCTGATATTGCACCAGGCGTATTTAGGCGGGGTCAACAGGACCGAATCCGAGATCAGGTTAGTCAACGACCGGGTGCTGCCGTTAGTTGCTAACGACTTGATGTGGGCCCTGAGCCTGCCGGAGGGGGCTGATCGTGACCACGAGTATTGTAATCCATCTATCAACGGCGGTGATGGAAGGATCGGACGGTTGCCGTGGTGTTTGGTTAGCGGGTACGGCGGCGACCCGCTGGTGGATAGGCAGAAGGAGCTCGCGGCATTGTTGGAGTCACGCGGGGTGCACGTGACGGCCCGGTTTGAGGAGGATGGTTTTCATGGTGCGGAATTTGACCCCAACAAGGCTGAGGCTTTCTATGAGATTGTTAAGGATTTTATCGGCTCTTGTTGTGCATACGGCGGCGACGTTGCAAAATCGACCATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0009056 GO:0016787
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

336

Amino Acids

36.43

Weight (kDa)

5.37

Isoelectric Point (pI)

35.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BD-FAE PF20434 78 - 187 1.3e-13 BD-FAE
Abhydrolase_3 PF07859 83 - 303 6e-54 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 423
AccI GTMKAC 1 cut(s) 544
AccII CGCG 5 cut(s) 515, 539, 549, 842, 861
AclWI GGATC 4 cut(s) 125, 513, 526, 776
AcoI YGGCCR 1 cut(s) 414
AcsI RAATTY 1 cut(s) 911
AcvI CACGTG 1 cut(s) 871
AcyI GRCGYC 1 cut(s) 405
AfaI GTAC 2 cut(s) 362, 803
AfiI CCNNNNNNNGG 7 cut(s) 160, 423, 642, 709, 820, 886, 925
AgsI TTSAA 1 cut(s) 208
AjnI CCWGG 3 cut(s) 422, 435, 600
AluBI AGCT 1 cut(s) 838
AluI AGCT 1 cut(s) 838
Alw21I GWGCWC 3 cut(s) 381, 840, 870
Alw26I GTCTC 1 cut(s) 371
Alw44I GTGCAC 1 cut(s) 866
AlwI GGATC 4 cut(s) 125, 513, 526, 776
Ama87I CYCGRG 1 cut(s) 374
AoxI GGCC 4 cut(s) 414, 438, 693, 876
ApaI GGGCCC 1 cut(s) 697
ApaLI GTGCAC 1 cut(s) 866
ApeKI GCWGC 3 cut(s) 282, 312, 664
ApoI RAATTY 1 cut(s) 911
AspLEI GCGC 3 cut(s) 287, 515, 551
AspS9I GGNCC 5 cut(s) 562, 627, 693, 694, 877
AsuC2I CCSGG 2 cut(s) 659, 880
AsuHPI GGTGA 3 cut(s) 64, 412, 770
AvaI CYCGRG 1 cut(s) 374
AvaII GGWCC 2 cut(s) 562, 627
BaeGI GKGCMC 2 cut(s) 697, 870
BamHI GGATCC 1 cut(s) 518
BanII GRGCYC 2 cut(s) 697, 840
BauI CACGAG 1 cut(s) 728
BbrPI CACGTG 1 cut(s) 871
Bbv12I GWGCWC 3 cut(s) 381, 840, 870
BbvCI CCTCAGC 1 cut(s) 930
BbvI GCAGC 3 cut(s) 294, 299, 651
BccI CCATC 6 cut(s) 56, 341, 425, 751, 755, 887
BceAI ACGGC 8 cut(s) 277, 392, 652, 766, 769, 820, 891, 997
BciT130I CCWGG 3 cut(s) 424, 437, 602
BcnI CCSGG 2 cut(s) 659, 880
BcoDI GTCTC 1 cut(s) 371
BfaI CTAG 2 cut(s) 344, 510
BfmI CTRYAG 1 cut(s) 280
BfoI RGCGCY 1 cut(s) 288
BglI GCCNNNNNGGC 1 cut(s) 413
Bme1390I CCNGG 5 cut(s) 424, 437, 602, 659, 880
Bme18I GGWCC 2 cut(s) 562, 627
BmeT110I CYCGRG 1 cut(s) 374
BmgT120I GGNCC 5 cut(s) 562, 627, 693, 694, 877
BmiI GGNNCC 2 cut(s) 520, 695
BmrFI CCNGG 5 cut(s) 424, 437, 602, 659, 880
Bpu10I CCTNAGC 2 cut(s) 698, 930
BpuMI CCSGG 2 cut(s) 659, 880
BsaAI YACGTR 1 cut(s) 871
BsaBI GATNNNNATC 2 cut(s) 129, 578
BsaHI GRCGYC 1 cut(s) 405
BsaJI CCNNGG 4 cut(s) 159, 259, 583, 782
BsaXI ACNNNNNCTCC 2 cut(s) 425, 455
Bsc4I CCNNNNNNNGG 7 cut(s) 160, 423, 642, 709, 820, 886, 925
Bse8I GATNNNNATC 2 cut(s) 129, 578
BseBI CCWGG 3 cut(s) 424, 437, 602
BseDI CCNNGG 4 cut(s) 159, 259, 583, 782
BseGI GGATG 5 cut(s) 140, 165, 255, 333, 898
BseJI GATNNNNATC 2 cut(s) 129, 578
BseLI CCNNNNNNNGG 7 cut(s) 160, 423, 642, 709, 820, 886, 925
BseMII CTCAG 3 cut(s) 308, 689, 921
BseRI GAGGAG 3 cut(s) 236, 433, 902
BseSI GKGCMC 2 cut(s) 697, 870
BseXI GCAGC 3 cut(s) 294, 299, 651
BseYI CCCAGC 1 cut(s) 337
Bsh1236I CGCG 5 cut(s) 515, 539, 549, 842, 861
Bsh1285I CGRYCG 1 cut(s) 658
BshFI GGCC 4 cut(s) 416, 440, 695, 878
BsiEI CGRYCG 1 cut(s) 658
BsiHKAI GWGCWC 3 cut(s) 381, 840, 870
BsiHKCI CYCGRG 1 cut(s) 374
BsiSI CCGG 3 cut(s) 658, 707, 880
BslI CCNNNNNNNGG 7 cut(s) 160, 423, 642, 709, 820, 886, 925
BsmAI GTCTC 1 cut(s) 371
BsmBI CGTCTC 1 cut(s) 371
BsnI GGCC 4 cut(s) 416, 440, 695, 878
BsoBI CYCGRG 1 cut(s) 374
Bsp120I GGGCCC 1 cut(s) 693
Bsp1286I GDGCHC 4 cut(s) 381, 697, 840, 870
Bsp143I GATC 6 cut(s) 130, 518, 573, 639, 718, 768
BspANI GGCC 4 cut(s) 416, 440, 695, 878
BspCNI CTCAG 3 cut(s) 307, 690, 922
BspFNI CGCG 5 cut(s) 515, 539, 549, 842, 861
BspLI GGNNCC 2 cut(s) 520, 695
BspMAI CTGCAG 1 cut(s) 284
BspPI GGATC 4 cut(s) 125, 513, 526, 776
BssECI CCNNGG 4 cut(s) 159, 259, 583, 782
BssMI GATC 6 cut(s) 130, 518, 573, 639, 718, 768
BssNI GRCGYC 1 cut(s) 405
BssSI CACGAG 1 cut(s) 728
BssT1I CCWWGG 1 cut(s) 159
Bst2BI CACGAG 1 cut(s) 728
Bst2UI CCWGG 3 cut(s) 424, 437, 602
Bst4CI ACNGT 2 cut(s) 365, 777
Bst6I CTCTTC 1 cut(s) 209
BstACI GRCGYC 1 cut(s) 405
BstBAI YACGTR 1 cut(s) 871
BstC8I GCNNGC 2 cut(s) 704, 840
BstDEI CTNAG 3 cut(s) 294, 698, 930
BstDSI CCRYGG 2 cut(s) 259, 782
BstENI CCTNNNNNAGG 2 cut(s) 158, 707
BstF5I GGATG 5 cut(s) 140, 165, 255, 333, 898
BstFNI CGCG 5 cut(s) 515, 539, 549, 842, 861
BstH2I RGCGCY 1 cut(s) 288
BstHHI GCGC 3 cut(s) 287, 515, 551
BstKTI GATC 6 cut(s) 133, 521, 576, 642, 721, 771
BstMAI GTCTC 1 cut(s) 371
BstMBI GATC 6 cut(s) 130, 518, 573, 639, 718, 768
BstMCI CGRYCG 1 cut(s) 658
BstMWI GCNNNNNNNGC 7 cut(s) 318, 413, 446, 536, 595, 804, 835
BstNI CCWGG 3 cut(s) 424, 437, 602
BstSCI CCNGG 5 cut(s) 422, 435, 600, 657, 878
BstSFI CTRYAG 1 cut(s) 280
BstSLI GKGCMC 2 cut(s) 697, 870
BstUI CGCG 5 cut(s) 515, 539, 549, 842, 861
BstV1I GCAGC 3 cut(s) 294, 299, 651
BstX2I RGATCY 1 cut(s) 518
BstYI RGATCY 1 cut(s) 518
BsuRI GGCC 4 cut(s) 416, 440, 695, 878
BtgI CCRYGG 2 cut(s) 259, 782
BtsCI GGATG 5 cut(s) 140, 165, 255, 333, 898
Cac8I GCNNGC 2 cut(s) 704, 840
CfoI GCGC 3 cut(s) 287, 515, 551
Cfr13I GGNCC 5 cut(s) 562, 627, 693, 694, 877
CseI GACGC 1 cut(s) 413
Csp6I GTAC 2 cut(s) 361, 802
CspCI CAANNNNNGTGG 2 cut(s) 716, 751
CviAII CATG 2 cut(s) 502, 902
CviQI GTAC 2 cut(s) 361, 802
DdeI CTNAG 3 cut(s) 294, 698, 930
DpnI GATC 6 cut(s) 132, 520, 575, 641, 720, 770
DpnII GATC 6 cut(s) 130, 518, 573, 639, 718, 768
EaeI YGGCCR 1 cut(s) 414
Eam1104I CTCTTC 1 cut(s) 209
EarI CTCTTC 1 cut(s) 209
EciI GGCGGA 1 cut(s) 188
Ecl136II GAGCTC 1 cut(s) 838
Eco130I CCWWGG 1 cut(s) 159
Eco147I AGGCCT 1 cut(s) 440
Eco24I GRGCYC 2 cut(s) 697, 840
Eco47I GGWCC 2 cut(s) 562, 627
Eco53kI GAGCTC 1 cut(s) 838
Eco72I CACGTG 1 cut(s) 871
Eco88I CYCGRG 1 cut(s) 374
EcoICRI GAGCTC 1 cut(s) 838
EcoNI CCTNNNNNAGG 2 cut(s) 158, 707
EcoO109I RGGNCCY 1 cut(s) 694
EcoRII CCWGG 3 cut(s) 422, 435, 600
EcoT14I CCWWGG 1 cut(s) 159
EcoT38I GRGCYC 2 cut(s) 697, 840
ErhI CCWWGG 1 cut(s) 159
Esp3I CGTCTC 1 cut(s) 371
FaeI CATG 2 cut(s) 505, 905
FaiI YATR 7 cut(s) 503, 560, 903, 942, 979, 1007, 1009
FatI CATG 2 cut(s) 501, 901
FauI CCCGC 9 cut(s) 83, 127, 397, 508, 532, 608, 791, 822, 854
FauNDI CATATG 1 cut(s) 1007
FblI GTMKAC 1 cut(s) 544
FokI GGATG 5 cut(s) 127, 152, 242, 320, 905
FriOI GRGCYC 2 cut(s) 697, 840
FspBI CTAG 2 cut(s) 344, 510
GlaI GCGC 3 cut(s) 286, 514, 550
GsaI CCCAGC 1 cut(s) 341
HaeII RGCGCY 1 cut(s) 288
HaeIII GGCC 4 cut(s) 416, 440, 695, 878
HapII CCGG 3 cut(s) 658, 707, 880
HgaI GACGC 1 cut(s) 413
HhaI GCGC 3 cut(s) 287, 515, 551
Hin1I GRCGYC 1 cut(s) 405
Hin1II CATG 2 cut(s) 505, 905
Hin6I GCGC 3 cut(s) 285, 513, 549
HinP1I GCGC 3 cut(s) 285, 513, 549
HincII GTYRAC 2 cut(s) 622, 652
HindII GTYRAC 2 cut(s) 622, 652
HinfI GANTC 5 cut(s) 110, 125, 458, 632, 854
HpaII CCGG 3 cut(s) 658, 707, 880
HphI GGTGA 3 cut(s) 64, 412, 770
Hpy166II GTNNAC 5 cut(s) 68, 545, 622, 652, 868
Hpy188I TCNGA 5 cut(s) 130, 556, 584, 637, 773
Hpy188III TCNNGA 2 cut(s) 173, 722
Hpy8I GTNNAC 5 cut(s) 68, 545, 622, 652, 868
Hpy99I CGWCG 5 cut(s) 359, 404, 407, 455, 992
HpyAV CCTTC 2 cut(s) 759, 826
HpyCH4III ACNGT 2 cut(s) 365, 777
HpyCH4IV ACGT 2 cut(s) 870, 990
HpyCH4V TGCA 5 cut(s) 282, 598, 868, 977, 995
HpyF10VI GCNNNNNNNGC 7 cut(s) 318, 413, 446, 536, 595, 804, 835
HpyF3I CTNAG 3 cut(s) 294, 698, 930
HpySE526I ACGT 2 cut(s) 870, 990
Hsp92I GRCGYC 1 cut(s) 405
Hsp92II CATG 2 cut(s) 505, 905
HspAI GCGC 3 cut(s) 285, 513, 549
Kzo9I GATC 6 cut(s) 130, 518, 573, 639, 718, 768
LmnI GCTCC 1 cut(s) 835
Lsp1109I GCAGC 3 cut(s) 294, 299, 651
MaeI CTAG 2 cut(s) 344, 510
MaeII ACGT 2 cut(s) 870, 990
MaeIII GTNAC 3 cut(s) 722, 855, 871
MalI GATC 6 cut(s) 132, 520, 575, 641, 720, 770
MboI GATC 6 cut(s) 130, 518, 573, 639, 718, 768
MboII GAAGA 1 cut(s) 196
MflI RGATCY 1 cut(s) 518
MhlI GDGCHC 4 cut(s) 381, 697, 840, 870
MluCI AATT 1 cut(s) 911
MlyI GAGTC 2 cut(s) 119, 863
MmeI TCCRAC 1 cut(s) 831
MseI TTAA 1 cut(s) 951
MspA1I CMGCKG 1 cut(s) 817
MspI CCGG 3 cut(s) 658, 707, 880
MspR9I CCNGG 5 cut(s) 424, 437, 602, 659, 880
MvaI CCWGG 3 cut(s) 424, 437, 602
MvnI CGCG 5 cut(s) 515, 539, 549, 842, 861
MwoI GCNNNNNNNGC 7 cut(s) 318, 413, 446, 536, 595, 804, 835
NciI CCSGG 2 cut(s) 659, 880
NdeI CATATG 1 cut(s) 1007
NdeII GATC 6 cut(s) 130, 518, 573, 639, 718, 768
NlaIII CATG 2 cut(s) 505, 905
NlaIV GGNNCC 2 cut(s) 520, 695
NmuCI GTSAC 3 cut(s) 722, 855, 871
PceI AGGCCT 1 cut(s) 440
PcsI WCGNNNNNNNCGW 2 cut(s) 405, 987
PfeI GAWTC 3 cut(s) 125, 458, 632
PflMI CCANNNNNTGG 1 cut(s) 423
PleI GAGTC 2 cut(s) 118, 862
PmaCI CACGTG 1 cut(s) 871
PmlI CACGTG 1 cut(s) 871
PpsI GAGTC 2 cut(s) 118, 862
Ppu21I YACGTR 1 cut(s) 871
Psp124BI GAGCTC 1 cut(s) 840
Psp6I CCWGG 3 cut(s) 422, 435, 600
PspCI CACGTG 1 cut(s) 871
PspFI CCCAGC 1 cut(s) 337
PspGI CCWGG 3 cut(s) 422, 435, 600
PspN4I GGNNCC 2 cut(s) 520, 695
PspOMI GGGCCC 1 cut(s) 693
PspPI GGNCC 5 cut(s) 562, 627, 693, 694, 877
PstI CTGCAG 1 cut(s) 284
PsuI RGATCY 1 cut(s) 518
RsaI GTAC 2 cut(s) 362, 803
RsaNI GTAC 2 cut(s) 361, 802
SacI GAGCTC 1 cut(s) 840
SaqAI TTAA 1 cut(s) 951
Sau3AI GATC 6 cut(s) 130, 518, 573, 639, 718, 768
Sau96I GGNCC 5 cut(s) 562, 627, 693, 694, 877
SchI GAGTC 2 cut(s) 119, 863
ScrFI CCNGG 5 cut(s) 424, 437, 602, 659, 880
SduI GDGCHC 4 cut(s) 381, 697, 840, 870
SetI ASST 8 cut(s) 194, 240, 425, 567, 647, 840, 873, 993
SfcI CTRYAG 1 cut(s) 280
SinI GGWCC 2 cut(s) 562, 627
Sse9I AATT 1 cut(s) 911
SseBI AGGCCT 1 cut(s) 440
SspMI CTAG 2 cut(s) 344, 510
SstI GAGCTC 1 cut(s) 840
StuI AGGCCT 1 cut(s) 440
StyD4I CCNGG 5 cut(s) 422, 435, 600, 657, 878
StyI CCWWGG 1 cut(s) 159
TaaI ACNGT 2 cut(s) 365, 777
TaiI ACGT 2 cut(s) 873, 993
TaqI TCGA 4 cut(s) 24, 174, 357, 1001
TaqII GACCGA 1 cut(s) 644
TasI AATT 1 cut(s) 911
TauI GCSGC 7 cut(s) 267, 270, 290, 395, 810, 845, 987
TfiI GAWTC 3 cut(s) 125, 458, 632
Tru1I TTAA 1 cut(s) 951
Tru9I TTAA 1 cut(s) 951
TseFI GTSAC 3 cut(s) 722, 855, 871
TseI GCWGC 3 cut(s) 282, 312, 664
Tsp45I GTSAC 3 cut(s) 722, 855, 871
TspDTI ATGAA 4 cut(s) 24, 262, 487, 890
TspGWI ACGGA 3 cut(s) 343, 466, 468
Van91I CCANNNNNTGG 1 cut(s) 423
VneI GTGCAC 1 cut(s) 866
VpaK11BI GGWCC 2 cut(s) 562, 627
XagI CCTNNNNNAGG 2 cut(s) 158, 707
XapI RAATTY 1 cut(s) 911
XmiI GTMKAC 1 cut(s) 544
XspI CTAG 2 cut(s) 344, 510
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.