Rw1G020230

carboxylesterase 8

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0005485
Physical Location & Seq
Forward (+)
43824094 .. 43825113
1020 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G020230.1

Sequence Viewer

Length: 1020 bp
ATGGCAGATGAACAATCCTCAATCGACCCCTACAAGTTCCTCAAAATCGTTCCCAATCCAGATGGCTCACTCACCCGCCTCGCCCCACTCTCAATCGCACCCCCAACCGAGTCAATCCCCGCCGATTCCGATCCCAACTCATCCCAACTCGTCCTCTCCAAGGACATCCCTCTCGACCCAAAATCCAAAACCTCCCTCCGCCTCTTCAAACCCCACAATCTCGCCCCAAACCCTAGCCTCCCTCTCCTCATCTACATCCACGGCGGCGGCTTCATCATCTGCAGTGCCGCATCTCAGCCTTTCCACCACTGCTGCTCTCGCCTCGCCCTCTCCATGCCAGCCCTAGTCCTCTCCGTCGAGTACCGTCTCGCCCCCGAGCACCGCCTCCCCGCCGCCTACGACGACGCCGTTTCGGCCATCACGTGGCTCAAAAACCAGGCCTCCTCTGCCGACGGAGATTCGTGGCTGAAAAACTCCGTTGATTTCTCCAAGTGTTTCATCATGGGAACTAGCGCGGGATCCAATATCGCCTACCACGCGGGTCTACGCGCTTCCGATATGGACCTCTCCCCGATCAAAATCCGAGGGCTGATATTGCACCAGGCGTATTTCGGCGGGGTCAACAGGACCGAATCCGAGATCAGGTTAGTCAACGACCGGGTGCTGCCGTTAGTTGCTAACGACTTGATGTGGGCCCTGAGCCTGCCGGAGGGGGCTGATCGTGACCACGAGTATTGTAATCCATCTATCAACGGCGGTGATGGAAGGATCGGACGGTTGCCGTGGTGTTTGGTGAGCGGGTACGGCGGCGATCCGCTGGTGGATAGGCAGAAGGAGATCGCGGCACTGTTGGAGTCACGCGGGGTGCACGTGACGGCCCGGTTTGAGGAAGGTGGTTTTCATGGTGCGGAATTTGACCCCAACAAGGCCGAGGCTTTCTATGAGATTGTTAAGGAATTTATCGGCTCTTGTTGTGCGGACGGCGGCGATGCTGTTGTCGTTGCAAAATCGACCATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0009056 GO:0016787
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

339

Amino Acids

36.6

Weight (kDa)

5.46

Isoelectric Point (pI)

38.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BD-FAE PF20434 78 - 187 1.1e-13 BD-FAE
Abhydrolase_3 PF07859 83 - 303 9.2e-54 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 423
AccBSI CCGCTC 1 cut(s) 798
AccI GTMKAC 1 cut(s) 544
AccII CGCG 5 cut(s) 515, 539, 549, 842, 861
AclWI GGATC 5 cut(s) 125, 513, 526, 776, 806
AcoI YGGCCR 1 cut(s) 414
AcsI RAATTY 2 cut(s) 911, 956
AcvI CACGTG 2 cut(s) 423, 871
AcyI GRCGYC 1 cut(s) 405
AfaI GTAC 2 cut(s) 362, 803
AfiI CCNNNNNNNGG 6 cut(s) 160, 423, 642, 709, 886, 925
AgsI TTSAA 1 cut(s) 208
AjnI CCWGG 2 cut(s) 435, 600
Alw21I GWGCWC 2 cut(s) 381, 870
Alw26I GTCTC 1 cut(s) 371
Alw44I GTGCAC 1 cut(s) 866
AlwI GGATC 5 cut(s) 125, 513, 526, 776, 806
Ama87I CYCGRG 1 cut(s) 374
AoxI GGCC 5 cut(s) 414, 438, 693, 876, 927
ApaI GGGCCC 1 cut(s) 697
ApaLI GTGCAC 1 cut(s) 866
ApeKI GCWGC 2 cut(s) 312, 664
ApoI RAATTY 2 cut(s) 911, 956
AspLEI GCGC 2 cut(s) 515, 551
AspS9I GGNCC 5 cut(s) 562, 627, 693, 694, 877
AsuC2I CCSGG 2 cut(s) 659, 880
AsuHPI GGTGA 3 cut(s) 64, 770, 805
AvaI CYCGRG 1 cut(s) 374
AvaII GGWCC 2 cut(s) 562, 627
BaeGI GKGCMC 2 cut(s) 697, 870
BamHI GGATCC 1 cut(s) 518
BanII GRGCYC 1 cut(s) 697
BauI CACGAG 1 cut(s) 728
BbrPI CACGTG 2 cut(s) 423, 871
Bbv12I GWGCWC 2 cut(s) 381, 870
BbvI GCAGC 2 cut(s) 299, 651
BccI CCATC 4 cut(s) 56, 425, 751, 755
BceAI ACGGC 8 cut(s) 277, 392, 652, 766, 769, 820, 891, 997
BciT130I CCWGG 2 cut(s) 437, 602
BcnI CCSGG 2 cut(s) 659, 880
BcoDI GTCTC 1 cut(s) 371
BfaI CTAG 3 cut(s) 234, 344, 510
BfmI CTRYAG 1 cut(s) 280
BglI GCCNNNNNGGC 1 cut(s) 413
BisI GCNGC 9 cut(s) 265, 268, 288, 313, 393, 665, 808, 843, 985
BlsI GCNGC 9 cut(s) 266, 269, 289, 314, 394, 666, 809, 844, 986
Bme1390I CCNGG 4 cut(s) 437, 602, 659, 880
Bme18I GGWCC 2 cut(s) 562, 627
BmeT110I CYCGRG 1 cut(s) 374
BmgT120I GGNCC 5 cut(s) 562, 627, 693, 694, 877
BmiI GGNNCC 2 cut(s) 520, 695
BmrFI CCNGG 4 cut(s) 437, 602, 659, 880
BmsI GCATC 2 cut(s) 299, 979
Bpu10I CCTNAGC 1 cut(s) 698
BpuMI CCSGG 2 cut(s) 659, 880
BsaAI YACGTR 2 cut(s) 423, 871
BsaBI GATNNNNATC 2 cut(s) 129, 578
BsaHI GRCGYC 1 cut(s) 405
BsaJI CCNNGG 5 cut(s) 159, 259, 583, 782, 930
BsaXI ACNNNNNCTCC 2 cut(s) 425, 455
Bsc4I CCNNNNNNNGG 6 cut(s) 160, 423, 642, 709, 886, 925
Bse8I GATNNNNATC 2 cut(s) 129, 578
BseBI CCWGG 2 cut(s) 437, 602
BseDI CCNNGG 5 cut(s) 159, 259, 583, 782, 930
BseGI GGATG 3 cut(s) 140, 165, 255
BseJI GATNNNNATC 2 cut(s) 129, 578
BseLI CCNNNNNNNGG 6 cut(s) 160, 423, 642, 709, 886, 925
BseMII CTCAG 2 cut(s) 308, 689
BseRI GAGGAG 2 cut(s) 236, 433
BseSI GKGCMC 2 cut(s) 697, 870
BseXI GCAGC 2 cut(s) 299, 651
Bsh1236I CGCG 5 cut(s) 515, 539, 549, 842, 861
Bsh1285I CGRYCG 1 cut(s) 658
BshFI GGCC 5 cut(s) 416, 440, 695, 878, 929
BsiEI CGRYCG 1 cut(s) 658
BsiHKAI GWGCWC 2 cut(s) 381, 870
BsiHKCI CYCGRG 1 cut(s) 374
BsiSI CCGG 3 cut(s) 658, 707, 880
BslI CCNNNNNNNGG 6 cut(s) 160, 423, 642, 709, 886, 925
BsmAI GTCTC 1 cut(s) 371
BsmBI CGTCTC 1 cut(s) 371
BsnI GGCC 5 cut(s) 416, 440, 695, 878, 929
BsoBI CYCGRG 1 cut(s) 374
Bsp120I GGGCCC 1 cut(s) 693
Bsp1286I GDGCHC 3 cut(s) 381, 697, 870
Bsp143I GATC 8 cut(s) 130, 518, 573, 639, 718, 768, 811, 837
BspANI GGCC 5 cut(s) 416, 440, 695, 878, 929
BspCNI CTCAG 2 cut(s) 307, 690
BspFNI CGCG 5 cut(s) 515, 539, 549, 842, 861
BspLI GGNNCC 2 cut(s) 520, 695
BspMAI CTGCAG 1 cut(s) 284
BspPI GGATC 5 cut(s) 125, 513, 526, 776, 806
BsrBI CCGCTC 1 cut(s) 798
BssECI CCNNGG 5 cut(s) 159, 259, 583, 782, 930
BssMI GATC 8 cut(s) 130, 518, 573, 639, 718, 768, 811, 837
BssNI GRCGYC 1 cut(s) 405
BssSI CACGAG 1 cut(s) 728
BssT1I CCWWGG 1 cut(s) 159
Bst2BI CACGAG 1 cut(s) 728
Bst2UI CCWGG 2 cut(s) 437, 602
Bst4CI ACNGT 3 cut(s) 365, 777, 849
Bst6I CTCTTC 1 cut(s) 209
BstACI GRCGYC 1 cut(s) 405
BstBAI YACGTR 2 cut(s) 423, 871
BstC8I GCNNGC 2 cut(s) 339, 704
BstDEI CTNAG 2 cut(s) 294, 698
BstDSI CCRYGG 2 cut(s) 259, 782
BstENI CCTNNNNNAGG 2 cut(s) 158, 707
BstF5I GGATG 3 cut(s) 140, 165, 255
BstFNI CGCG 5 cut(s) 515, 539, 549, 842, 861
BstHHI GCGC 2 cut(s) 515, 551
BstKTI GATC 8 cut(s) 133, 521, 576, 642, 721, 771, 814, 840
BstMAI GTCTC 1 cut(s) 371
BstMBI GATC 8 cut(s) 130, 518, 573, 639, 718, 768, 811, 837
BstMCI CGRYCG 1 cut(s) 658
BstMWI GCNNNNNNNGC 6 cut(s) 318, 413, 446, 536, 595, 804
BstNI CCWGG 2 cut(s) 437, 602
BstSCI CCNGG 4 cut(s) 435, 600, 657, 878
BstSFI CTRYAG 1 cut(s) 280
BstSLI GKGCMC 2 cut(s) 697, 870
BstUI CGCG 5 cut(s) 515, 539, 549, 842, 861
BstV1I GCAGC 2 cut(s) 299, 651
BstX2I RGATCY 1 cut(s) 518
BstYI RGATCY 1 cut(s) 518
BsuRI GGCC 5 cut(s) 416, 440, 695, 878, 929
BtgI CCRYGG 2 cut(s) 259, 782
BtgZI GCGATG 1 cut(s) 1002
BtsCI GGATG 3 cut(s) 140, 165, 255
BtsI GCAGTG 2 cut(s) 289, 307
BtsIMutI CAGTG 3 cut(s) 289, 307, 845
Cac8I GCNNGC 2 cut(s) 339, 704
CfoI GCGC 2 cut(s) 515, 551
Cfr13I GGNCC 5 cut(s) 562, 627, 693, 694, 877
CseI GACGC 1 cut(s) 413
Csp6I GTAC 2 cut(s) 361, 802
CspCI CAANNNNNGTGG 2 cut(s) 716, 751
CviAII CATG 3 cut(s) 334, 502, 902
CviQI GTAC 2 cut(s) 361, 802
DdeI CTNAG 2 cut(s) 294, 698
DpnI GATC 8 cut(s) 132, 520, 575, 641, 720, 770, 813, 839
DpnII GATC 8 cut(s) 130, 518, 573, 639, 718, 768, 811, 837
EaeI YGGCCR 1 cut(s) 414
Eam1104I CTCTTC 1 cut(s) 209
EarI CTCTTC 1 cut(s) 209
EciI GGCGGA 1 cut(s) 188
Eco130I CCWWGG 1 cut(s) 159
Eco147I AGGCCT 1 cut(s) 440
Eco24I GRGCYC 1 cut(s) 697
Eco47I GGWCC 2 cut(s) 562, 627
Eco72I CACGTG 2 cut(s) 423, 871
Eco88I CYCGRG 1 cut(s) 374
EcoNI CCTNNNNNAGG 2 cut(s) 158, 707
EcoO109I RGGNCCY 1 cut(s) 694
EcoRII CCWGG 2 cut(s) 435, 600
EcoT14I CCWWGG 1 cut(s) 159
EcoT38I GRGCYC 1 cut(s) 697
ErhI CCWWGG 1 cut(s) 159
Esp3I CGTCTC 1 cut(s) 371
FaeI CATG 3 cut(s) 337, 505, 905
FaiI YATR 7 cut(s) 335, 503, 560, 903, 942, 1016, 1018
FatI CATG 3 cut(s) 333, 501, 901
FauI CCCGC 8 cut(s) 83, 127, 397, 508, 532, 608, 791, 854
FauNDI CATATG 1 cut(s) 1016
FblI GTMKAC 1 cut(s) 544
Fnu4HI GCNGC 9 cut(s) 265, 268, 288, 313, 393, 665, 808, 843, 985
FokI GGATG 3 cut(s) 127, 152, 242
FriOI GRGCYC 1 cut(s) 697
Fsp4HI GCNGC 9 cut(s) 265, 268, 288, 313, 393, 665, 808, 843, 985
FspBI CTAG 3 cut(s) 234, 344, 510
GlaI GCGC 2 cut(s) 514, 550
GluI GCNGC 9 cut(s) 265, 268, 288, 313, 393, 665, 808, 843, 985
HaeIII GGCC 5 cut(s) 416, 440, 695, 878, 929
HapII CCGG 3 cut(s) 658, 707, 880
HgaI GACGC 1 cut(s) 413
HhaI GCGC 2 cut(s) 515, 551
Hin1I GRCGYC 1 cut(s) 405
Hin1II CATG 3 cut(s) 337, 505, 905
Hin6I GCGC 2 cut(s) 513, 549
HinP1I GCGC 2 cut(s) 513, 549
HincII GTYRAC 2 cut(s) 622, 652
HindII GTYRAC 2 cut(s) 622, 652
HinfI GANTC 5 cut(s) 110, 125, 458, 632, 854
HpaII CCGG 3 cut(s) 658, 707, 880
HphI GGTGA 3 cut(s) 64, 770, 805
Hpy166II GTNNAC 4 cut(s) 545, 622, 652, 868
Hpy188I TCNGA 5 cut(s) 130, 556, 584, 637, 773
Hpy188III TCNNGA 3 cut(s) 59, 173, 722
Hpy8I GTNNAC 4 cut(s) 545, 622, 652, 868
Hpy99I CGWCG 4 cut(s) 359, 404, 407, 455
HpyAV CCTTC 3 cut(s) 759, 826, 884
HpyCH4III ACNGT 3 cut(s) 365, 777, 849
HpyCH4IV ACGT 2 cut(s) 422, 870
HpyCH4V TGCA 4 cut(s) 282, 598, 868, 1004
HpyF10VI GCNNNNNNNGC 6 cut(s) 318, 413, 446, 536, 595, 804
HpyF3I CTNAG 2 cut(s) 294, 698
HpySE526I ACGT 2 cut(s) 422, 870
Hsp92I GRCGYC 1 cut(s) 405
Hsp92II CATG 3 cut(s) 337, 505, 905
HspAI GCGC 2 cut(s) 513, 549
Kzo9I GATC 8 cut(s) 130, 518, 573, 639, 718, 768, 811, 837
Lsp1109I GCAGC 2 cut(s) 299, 651
LweI GCATC 2 cut(s) 299, 979
MaeI CTAG 3 cut(s) 234, 344, 510
MaeII ACGT 2 cut(s) 422, 870
MaeIII GTNAC 3 cut(s) 722, 855, 871
MalI GATC 8 cut(s) 132, 520, 575, 641, 720, 770, 813, 839
MbiI CCGCTC 1 cut(s) 798
MboI GATC 8 cut(s) 130, 518, 573, 639, 718, 768, 811, 837
MboII GAAGA 1 cut(s) 196
MflI RGATCY 1 cut(s) 518
MhlI GDGCHC 3 cut(s) 381, 697, 870
MluCI AATT 2 cut(s) 911, 956
MlyI GAGTC 2 cut(s) 119, 863
MmeI TCCRAC 1 cut(s) 831
MseI TTAA 1 cut(s) 951
MspA1I CMGCKG 1 cut(s) 817
MspI CCGG 3 cut(s) 658, 707, 880
MspR9I CCNGG 4 cut(s) 437, 602, 659, 880
MvaI CCWGG 2 cut(s) 437, 602
MvnI CGCG 5 cut(s) 515, 539, 549, 842, 861
MwoI GCNNNNNNNGC 6 cut(s) 318, 413, 446, 536, 595, 804
NciI CCSGG 2 cut(s) 659, 880
NdeI CATATG 1 cut(s) 1016
NdeII GATC 8 cut(s) 130, 518, 573, 639, 718, 768, 811, 837
NlaIII CATG 3 cut(s) 337, 505, 905
NlaIV GGNNCC 2 cut(s) 520, 695
NmeAIII GCCGAG 1 cut(s) 955
NmuCI GTSAC 3 cut(s) 722, 855, 871
PceI AGGCCT 1 cut(s) 440
PcsI WCGNNNNNNNCGW 2 cut(s) 405, 419
PfeI GAWTC 3 cut(s) 125, 458, 632
PflMI CCANNNNNTGG 1 cut(s) 423
PkrI GCNGC 9 cut(s) 266, 269, 289, 314, 394, 666, 809, 844, 986
PleI GAGTC 2 cut(s) 118, 862
PmaCI CACGTG 2 cut(s) 423, 871
PmlI CACGTG 2 cut(s) 423, 871
PpsI GAGTC 2 cut(s) 118, 862
Ppu21I YACGTR 2 cut(s) 423, 871
Psp6I CCWGG 2 cut(s) 435, 600
PspCI CACGTG 2 cut(s) 423, 871
PspGI CCWGG 2 cut(s) 435, 600
PspN4I GGNNCC 2 cut(s) 520, 695
PspOMI GGGCCC 1 cut(s) 693
PspPI GGNCC 5 cut(s) 562, 627, 693, 694, 877
PstI CTGCAG 1 cut(s) 284
PsuI RGATCY 1 cut(s) 518
RsaI GTAC 2 cut(s) 362, 803
RsaNI GTAC 2 cut(s) 361, 802
SaqAI TTAA 1 cut(s) 951
SatI GCNGC 9 cut(s) 265, 268, 288, 313, 393, 665, 808, 843, 985
Sau3AI GATC 8 cut(s) 130, 518, 573, 639, 718, 768, 811, 837
Sau96I GGNCC 5 cut(s) 562, 627, 693, 694, 877
SchI GAGTC 2 cut(s) 119, 863
ScrFI CCNGG 4 cut(s) 437, 602, 659, 880
SduI GDGCHC 3 cut(s) 381, 697, 870
SetI ASST 6 cut(s) 194, 425, 567, 647, 873, 895
SfaNI GCATC 2 cut(s) 299, 979
SfcI CTRYAG 1 cut(s) 280
SinI GGWCC 2 cut(s) 562, 627
Sse9I AATT 2 cut(s) 911, 956
SseBI AGGCCT 1 cut(s) 440
SspMI CTAG 3 cut(s) 234, 344, 510
StuI AGGCCT 1 cut(s) 440
StyD4I CCNGG 4 cut(s) 435, 600, 657, 878
StyI CCWWGG 1 cut(s) 159
TaaI ACNGT 3 cut(s) 365, 777, 849
TaiI ACGT 2 cut(s) 425, 873
TaqI TCGA 4 cut(s) 24, 174, 357, 1010
TaqII GACCGA 1 cut(s) 644
TasI AATT 2 cut(s) 911, 956
TauI GCSGC 7 cut(s) 267, 270, 290, 395, 810, 845, 987
TfiI GAWTC 3 cut(s) 125, 458, 632
Tru1I TTAA 1 cut(s) 951
Tru9I TTAA 1 cut(s) 951
TscAI CASTG 3 cut(s) 289, 314, 852
TseFI GTSAC 3 cut(s) 722, 855, 871
TseI GCWGC 2 cut(s) 312, 664
Tsp45I GTSAC 3 cut(s) 722, 855, 871
TspDTI ATGAA 4 cut(s) 24, 262, 487, 890
TspGWI ACGGA 3 cut(s) 343, 466, 468
TspRI CASTG 3 cut(s) 289, 314, 852
Van91I CCANNNNNTGG 1 cut(s) 423
VneI GTGCAC 1 cut(s) 866
VpaK11BI GGWCC 2 cut(s) 562, 627
XagI CCTNNNNNAGG 2 cut(s) 158, 707
XapI RAATTY 2 cut(s) 911, 956
XmiI GTMKAC 1 cut(s) 544
XspI CTAG 3 cut(s) 234, 344, 510
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.