MD02G1198600.v1.1

carboxylesterase 8

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0005485
Physical Location & Seq
Reverse (-)
19506971 .. 19507423
453 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1198600.v1.1.491

Sequence Viewer

Length: 453 bp
ATGAAGATCCGAGGGCTGATACTAAACCAGCCTTTTTTCGGTGGGGTCCATAGGACTGAATCCGAGATGAGGTTGATCAACGATCGGACATTTTGCCAGCTGGTCGTTAACGACTTGATGTGGGCCACGGCTTTGCCAAAGGGAGTCGATCGGGATCACGAGTATTGTAATCCGACGGCGGGAGTTGGGGATGGGAGGATCGAACGGTTGCCGAGGCGTTTGGTTAGAGGGTACGGTGGGGACCCGCTGATGGATAGGCAGAAAGAGTTTGTGGCGTTACTTGAGTCACGTGGGGCGCACGTGGTGGCCAAGTTTGACGAGGAGGGGTTTCATGGCGTGGAGCTCTTCGACCCCAGCATGGCCCAAGTTCTGTACGACATTCTTAGGGATTTTATCGAGTCGTGCAGTACATACGACGGTGATAATGTTGTCGTTTCAAAATCGGCCACGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0009056 GO:0016787
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

151

Amino Acids

16.91

Weight (kDa)

5.34

Isoelectric Point (pI)

38.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydrolase_3 PF07859 2 - 113 2.5e-12 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 179, 245
AclWI GGATC 2 cut(s) 162, 206
AcoI YGGCCR 2 cut(s) 306, 444
AcvI CACGTG 3 cut(s) 290, 301, 450
AdeI CACNNNGTG 1 cut(s) 304
AfaI GTAC 3 cut(s) 233, 374, 409
AfiI CCNNNNNNNGG 5 cut(s) 38, 69, 179, 250, 358
AgsI TTSAA 1 cut(s) 438
AluBI AGCT 2 cut(s) 100, 343
AluI AGCT 2 cut(s) 100, 343
Alw21I GWGCWC 1 cut(s) 345
AlwI GGATC 2 cut(s) 162, 206
AoxI GGCC 4 cut(s) 123, 306, 360, 444
AspLEI GCGC 1 cut(s) 298
AspS9I GGNCC 4 cut(s) 46, 123, 241, 361
AsuHPI GGTGA 1 cut(s) 431
AvaII GGWCC 2 cut(s) 46, 241
BalI TGGCCA 1 cut(s) 308
BanII GRGCYC 1 cut(s) 345
BauI CACGAG 1 cut(s) 158
BbrPI CACGTG 3 cut(s) 290, 301, 450
Bbv12I GWGCWC 1 cut(s) 345
BccI CCATC 2 cut(s) 185, 244
BceAI ACGGC 2 cut(s) 144, 192
BclI TGATCA 1 cut(s) 75
Bme18I GGWCC 2 cut(s) 46, 241
BmgT120I GGNCC 4 cut(s) 46, 123, 241, 361
BmiI GGNNCC 3 cut(s) 47, 242, 243
BpuEI CTTGAG 1 cut(s) 302
BsaAI YACGTR 3 cut(s) 290, 301, 450
BsaBI GATNNNNATC 1 cut(s) 153
BsaJI CCNNGG 3 cut(s) 10, 126, 212
Bsc4I CCNNNNNNNGG 5 cut(s) 38, 69, 179, 250, 358
Bse8I GATNNNNATC 1 cut(s) 153
BseDI CCNNGG 3 cut(s) 10, 126, 212
BseGI GGATG 1 cut(s) 196
BseJI GATNNNNATC 1 cut(s) 153
BseLI CCNNNNNNNGG 5 cut(s) 38, 69, 179, 250, 358
BseRI GAGGAG 1 cut(s) 335
BseYI CCCAGC 1 cut(s) 353
BsgI GTGCAG 1 cut(s) 424
Bsh1285I CGRYCG 2 cut(s) 85, 151
BshFI GGCC 4 cut(s) 125, 308, 362, 446
BsiEI CGRYCG 2 cut(s) 85, 151
BsiHKAI GWGCWC 1 cut(s) 345
BslFI GGGAC 1 cut(s) 254
BslI CCNNNNNNNGG 5 cut(s) 38, 69, 179, 250, 358
BsmFI GGGAC 1 cut(s) 254
BsnI GGCC 4 cut(s) 125, 308, 362, 446
Bsp1286I GDGCHC 1 cut(s) 345
Bsp143I GATC 6 cut(s) 6, 75, 82, 148, 154, 198
BspACI CCGC 2 cut(s) 179, 245
BspANI GGCC 4 cut(s) 125, 308, 362, 446
BspLI GGNNCC 3 cut(s) 47, 242, 243
BspPI GGATC 2 cut(s) 162, 206
BspQI GCTCTTC 1 cut(s) 350
BssECI CCNNGG 3 cut(s) 10, 126, 212
BssMI GATC 6 cut(s) 6, 75, 82, 148, 154, 198
BssSI CACGAG 1 cut(s) 158
Bst2BI CACGAG 1 cut(s) 158
Bst4CI ACNGT 3 cut(s) 207, 236, 419
Bst6I CTCTTC 1 cut(s) 350
BstBAI YACGTR 3 cut(s) 290, 301, 450
BstC8I GCNNGC 1 cut(s) 98
BstDEI CTNAG 1 cut(s) 383
BstDSI CCRYGG 1 cut(s) 126
BstF5I GGATG 1 cut(s) 196
BstHHI GCGC 1 cut(s) 298
BstKTI GATC 6 cut(s) 9, 78, 85, 151, 157, 201
BstMBI GATC 6 cut(s) 6, 75, 82, 148, 154, 198
BstMCI CGRYCG 2 cut(s) 85, 151
BstX2I RGATCY 1 cut(s) 6
BstYI RGATCY 1 cut(s) 6
BsuRI GGCC 4 cut(s) 125, 308, 362, 446
BtgI CCRYGG 1 cut(s) 126
BtsCI GGATG 1 cut(s) 196
Cac8I GCNNGC 1 cut(s) 98
CfoI GCGC 1 cut(s) 298
Cfr13I GGNCC 4 cut(s) 46, 123, 241, 361
Csp6I GTAC 3 cut(s) 232, 373, 408
CviAII CATG 2 cut(s) 332, 358
CviJI RGCY 9 cut(s) 16, 31, 100, 125, 131, 308, 343, 362, 446
CviKI_1 RGCY 9 cut(s) 16, 31, 100, 125, 131, 308, 343, 362, 446
CviQI GTAC 3 cut(s) 232, 373, 408
DdeI CTNAG 1 cut(s) 383
DpnI GATC 6 cut(s) 8, 77, 84, 150, 156, 200
DpnII GATC 6 cut(s) 6, 75, 82, 148, 154, 198
DraIII CACNNNGTG 1 cut(s) 304
EaeI YGGCCR 2 cut(s) 306, 444
Eam1104I CTCTTC 1 cut(s) 350
EarI CTCTTC 1 cut(s) 350
Ecl136II GAGCTC 1 cut(s) 343
Eco24I GRGCYC 1 cut(s) 345
Eco47I GGWCC 2 cut(s) 46, 241
Eco53kI GAGCTC 1 cut(s) 343
Eco72I CACGTG 3 cut(s) 290, 301, 450
EcoICRI GAGCTC 1 cut(s) 343
EcoO109I RGGNCCY 1 cut(s) 241
EcoT38I GRGCYC 1 cut(s) 345
FaeI CATG 2 cut(s) 335, 361
FaiI YATR 4 cut(s) 51, 333, 359, 412
FaqI GGGAC 1 cut(s) 254
FatI CATG 2 cut(s) 331, 357
FauI CCCGC 2 cut(s) 172, 252
FbaI TGATCA 1 cut(s) 75
FokI GGATG 1 cut(s) 203
FriOI GRGCYC 1 cut(s) 345
GlaI GCGC 1 cut(s) 297
GsaI CCCAGC 1 cut(s) 357
HaeIII GGCC 4 cut(s) 125, 308, 362, 446
HhaI GCGC 1 cut(s) 298
Hin1II CATG 2 cut(s) 335, 361
Hin6I GCGC 1 cut(s) 296
HinP1I GCGC 1 cut(s) 296
HincII GTYRAC 1 cut(s) 109
HindII GTYRAC 1 cut(s) 109
HinfI GANTC 4 cut(s) 59, 144, 284, 398
HpaI GTTAAC 1 cut(s) 109
HphI GGTGA 1 cut(s) 431
Hpy166II GTNNAC 1 cut(s) 109
Hpy188I TCNGA 4 cut(s) 11, 64, 87, 174
Hpy188III TCNNGA 2 cut(s) 152, 158
Hpy8I GTNNAC 1 cut(s) 109
Hpy99I CGWCG 2 cut(s) 178, 419
HpyCH4III ACNGT 3 cut(s) 207, 236, 419
HpyCH4IV ACGT 3 cut(s) 289, 300, 449
HpyCH4V TGCA 1 cut(s) 405
HpyF3I CTNAG 1 cut(s) 383
HpySE526I ACGT 3 cut(s) 289, 300, 449
Hsp92II CATG 2 cut(s) 335, 361
HspAI GCGC 1 cut(s) 296
KflI GGGWCCC 1 cut(s) 241
Ksp22I TGATCA 1 cut(s) 75
KspAI GTTAAC 1 cut(s) 109
Kzo9I GATC 6 cut(s) 6, 75, 82, 148, 154, 198
LguI GCTCTTC 1 cut(s) 350
LmnI GCTCC 1 cut(s) 340
LpnPI CCDG 4 cut(s) 41, 86, 110, 367
MaeII ACGT 3 cut(s) 289, 300, 449
MaeIII GTNAC 2 cut(s) 276, 285
MalI GATC 6 cut(s) 8, 77, 84, 150, 156, 200
MboI GATC 6 cut(s) 6, 75, 82, 148, 154, 198
MboII GAAGA 2 cut(s) 16, 337
MflI RGATCY 1 cut(s) 6
MhlI GDGCHC 1 cut(s) 345
MlsI TGGCCA 1 cut(s) 308
MluNI TGGCCA 1 cut(s) 308
MlyI GAGTC 3 cut(s) 153, 293, 407
MmeI TCCRAC 1 cut(s) 197
MnlI CCTC 7 cut(s) 5, 63, 189, 207, 221, 313, 316
Mox20I TGGCCA 1 cut(s) 308
MscI TGGCCA 1 cut(s) 308
MseI TTAA 1 cut(s) 108
Msp20I TGGCCA 1 cut(s) 308
MspA1I CMGCKG 2 cut(s) 100, 247
NdeII GATC 6 cut(s) 6, 75, 82, 148, 154, 198
NlaIII CATG 2 cut(s) 335, 361
NlaIV GGNNCC 3 cut(s) 47, 242, 243
NmeAIII GCCGAG 1 cut(s) 237
NmuCI GTSAC 1 cut(s) 285
PciSI GCTCTTC 1 cut(s) 350
PfeI GAWTC 1 cut(s) 59
Ple19I CGATCG 2 cut(s) 85, 151
PleI GAGTC 3 cut(s) 152, 292, 406
PmaCI CACGTG 3 cut(s) 290, 301, 450
PmlI CACGTG 3 cut(s) 290, 301, 450
PpsI GAGTC 3 cut(s) 152, 292, 406
Ppu21I YACGTR 3 cut(s) 290, 301, 450
PpuMI RGGWCCY 1 cut(s) 241
Psp124BI GAGCTC 1 cut(s) 345
Psp5II RGGWCCY 1 cut(s) 241
PspCI CACGTG 3 cut(s) 290, 301, 450
PspFI CCCAGC 1 cut(s) 353
PspN4I GGNNCC 3 cut(s) 47, 242, 243
PspPI GGNCC 4 cut(s) 46, 123, 241, 361
PspPPI RGGWCCY 1 cut(s) 241
PsuI RGATCY 1 cut(s) 6
PvuI CGATCG 2 cut(s) 85, 151
PvuII CAGCTG 1 cut(s) 100
RsaI GTAC 3 cut(s) 233, 374, 409
RsaNI GTAC 3 cut(s) 232, 373, 408
SacI GAGCTC 1 cut(s) 345
SapI GCTCTTC 1 cut(s) 350
SaqAI TTAA 1 cut(s) 108
Sau3AI GATC 6 cut(s) 6, 75, 82, 148, 154, 198
Sau96I GGNCC 4 cut(s) 46, 123, 241, 361
SchI GAGTC 3 cut(s) 153, 293, 407
SduI GDGCHC 1 cut(s) 345
SetI ASST 6 cut(s) 74, 102, 292, 303, 345, 452
SinI GGWCC 2 cut(s) 46, 241
SmlI CTYRAG 1 cut(s) 281
SmoI CTYRAG 1 cut(s) 281
SsiI CCGC 2 cut(s) 179, 245
SstI GAGCTC 1 cut(s) 345
TaaI ACNGT 3 cut(s) 207, 236, 419
TaiI ACGT 3 cut(s) 292, 303, 452
TaqI TCGA 4 cut(s) 147, 201, 348, 396
TatI WGTACW 1 cut(s) 407
TfiI GAWTC 1 cut(s) 59
Tru1I TTAA 1 cut(s) 108
Tru9I TTAA 1 cut(s) 108
TseFI GTSAC 1 cut(s) 285
Tsp45I GTSAC 1 cut(s) 285
TspDTI ATGAA 2 cut(s) 17, 320
VpaK11BI GGWCC 2 cut(s) 46, 241
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.