Rroxscaffold_4G00303850

carboxylesterase 8

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0005485
Physical Location & Seq
Reverse (-)
24312988 .. 24318315
5328 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00303850.1

Sequence Viewer

Length: 972 bp
ATGGGAACTAGCGCGGGATCCACTATCGCCTACCACGCGGGTCTACGGGCTTCCGATATGGACCTCTCCCCAATCAAAATCCGAGGGCTGATATTGCACCAGGCGTATTTCGGCGGGGTCAACAGAACCGAATCCGAGATCAGGTTAGTCAACGACCGGGTGCTGCCGTTAGTTGCTAACGACTTGATGTGGGCCCTGAGCCTGCCGGAGGGGGCGGATCGTGACCACGAGTATTGTAATCCATCTATCAACGGCGGTGATGGAAGGATCGGACGGTTGCCGTGGTGTTTGGTTAGCGGGTACGGCGGCGACCCGCTGTTGGATAGGCAGAAGGAGATCGCGGCATTGTTGGGAGTCACGCGGGGTGCACGTGACGGCCCGATCACCCAGATTTACTTTACGGTTAAGATGTATCATGGTGGATACATTAGTGGAAGCAATTATATTGGTGGTCAAGTGAACTACCGTGACTATGTAGATAAGGATCGAATGTCACTTGTAGAGGTTGATAGGATGGTCGAGGTTAAACCGAACTACGGTGGAAGGAGAATAGATTATTGGTACAAGTATAGGACCGAGGATGATGAATACGGAGATGATGATTCCGCAAATGAAGATTATAGGCCACATTTTGAGGATGATGAGTATGGGGATTATGGTTGGGATGATGATTGGCTGGAAGATATGGAAGGTGAATTTGATGTCTTTGTAAGAAAAAAGGCCACCAAGACAAAGAATGAGTTAAGGCAGAAGGCAAGGGAAAGGGCTGAAGTACTAAAGAAAAAAAGGGATGAGAAGAAGGCTGCCACAGCTAGAGCTGCTCCAACAAATGCAAGAACTGCTTCTACAAATGCAAGAGCTGCTGTCGCTTTAACCTCAAGAGCTGCTCCTGCTTCAAGCTCAAGACCTCGCTCTTGCTTCAACCTCGAAAGGAACTGCTGCAAAAGGTACAGCTCCTGTTTCAAGCTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0009056 GO:0016787
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

323

Amino Acids

36.46

Weight (kDa)

6.56

Isoelectric Point (pI)

31.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Abhydrolase_3 PF07859 1 - 118 1.2e-13 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 43
AccII CGCG 4 cut(s) 14, 38, 341, 361
AclWI GGATC 5 cut(s) 12, 25, 225, 275, 492
AcsI RAATTY 1 cut(s) 695
AcuI CTGAAG 1 cut(s) 789
AcvI CACGTG 1 cut(s) 371
AfaI GTAC 4 cut(s) 302, 563, 774, 950
AfiI CCNNNNNNNGG 4 cut(s) 141, 208, 319, 536
AgsI TTSAA 3 cut(s) 897, 922, 964
AjnI CCWGG 1 cut(s) 99
AjuI GAANNNNNNNTTGG 2 cut(s) 541, 573
AluBI AGCT 7 cut(s) 812, 818, 860, 884, 900, 954, 967
AluI AGCT 7 cut(s) 812, 818, 860, 884, 900, 954, 967
Alw21I GWGCWC 1 cut(s) 370
Alw44I GTGCAC 1 cut(s) 366
AlwI GGATC 5 cut(s) 12, 25, 225, 275, 492
AlwNI CAGNNNCTG 1 cut(s) 957
AoxI GGCC 4 cut(s) 192, 376, 623, 720
ApaI GGGCCC 1 cut(s) 196
ApaLI GTGCAC 1 cut(s) 366
ApeKI GCWGC 6 cut(s) 163, 803, 818, 860, 884, 939
ApoI RAATTY 1 cut(s) 695
Asp700I GAANNNNTTC 1 cut(s) 841
AspLEI GCGC 1 cut(s) 14
AspS9I GGNCC 5 cut(s) 61, 192, 193, 377, 573
AsuC2I CCSGG 1 cut(s) 158
AsuHPI GGTGA 3 cut(s) 269, 376, 704
AvaII GGWCC 2 cut(s) 61, 573
BaeGI GKGCMC 2 cut(s) 196, 370
BaeI ACNNNNGTAYC 2 cut(s) 415, 448
BamHI GGATCC 1 cut(s) 17
BanII GRGCYC 1 cut(s) 196
BauI CACGAG 1 cut(s) 227
BbrPI CACGTG 1 cut(s) 371
Bbv12I GWGCWC 1 cut(s) 370
BbvI GCAGC 6 cut(s) 150, 790, 805, 847, 871, 926
BccI CCATC 3 cut(s) 250, 254, 508
BceAI ACGGC 5 cut(s) 151, 265, 268, 319, 391
BciT130I CCWGG 1 cut(s) 101
BciVI GTATCC 1 cut(s) 416
BcnI CCSGG 1 cut(s) 158
BfaI CTAG 3 cut(s) 9, 813, 970
BfuI GTATCC 1 cut(s) 416
BisI GCNGC 8 cut(s) 164, 307, 342, 804, 819, 861, 885, 940
BlsI GCNGC 8 cut(s) 165, 308, 343, 805, 820, 862, 886, 941
BmcAI AGTACT 1 cut(s) 774
Bme1390I CCNGG 2 cut(s) 101, 158
Bme18I GGWCC 2 cut(s) 61, 573
BmgT120I GGNCC 5 cut(s) 61, 192, 193, 377, 573
BmiI GGNNCC 2 cut(s) 19, 194
BmrFI CCNGG 2 cut(s) 101, 158
Bpu10I CCTNAGC 1 cut(s) 197
BpuEI CTTGAG 2 cut(s) 862, 886
BpuMI CCSGG 1 cut(s) 158
BsaAI YACGTR 1 cut(s) 371
BsaBI GATNNNNATC 1 cut(s) 483
BsaJI CCNNGG 3 cut(s) 82, 281, 576
Bsc4I CCNNNNNNNGG 4 cut(s) 141, 208, 319, 536
Bse8I GATNNNNATC 1 cut(s) 483
BseBI CCWGG 1 cut(s) 101
BseDI CCNNGG 3 cut(s) 82, 281, 576
BseGI GGATG 5 cut(s) 519, 586, 643, 670, 796
BseJI GATNNNNATC 1 cut(s) 483
BseLI CCNNNNNNNGG 4 cut(s) 141, 208, 319, 536
BseMII CTCAG 1 cut(s) 188
BseSI GKGCMC 2 cut(s) 196, 370
BseXI GCAGC 6 cut(s) 150, 790, 805, 847, 871, 926
Bsh1236I CGCG 4 cut(s) 14, 38, 341, 361
Bsh1285I CGRYCG 1 cut(s) 157
BshFI GGCC 4 cut(s) 194, 378, 625, 722
BsiEI CGRYCG 1 cut(s) 157
BsiHKAI GWGCWC 1 cut(s) 370
BsiSI CCGG 2 cut(s) 157, 206
BslI CCNNNNNNNGG 4 cut(s) 141, 208, 319, 536
BsnI GGCC 4 cut(s) 194, 378, 625, 722
Bsp120I GGGCCC 1 cut(s) 192
Bsp1286I GDGCHC 2 cut(s) 196, 370
Bsp143I GATC 7 cut(s) 17, 138, 217, 267, 336, 381, 484
BspANI GGCC 4 cut(s) 194, 378, 625, 722
BspCNI CTCAG 1 cut(s) 189
BspFNI CGCG 4 cut(s) 14, 38, 341, 361
BspLI GGNNCC 2 cut(s) 19, 194
BspPI GGATC 5 cut(s) 12, 25, 225, 275, 492
BssECI CCNNGG 3 cut(s) 82, 281, 576
BssMI GATC 7 cut(s) 17, 138, 217, 267, 336, 381, 484
BssSI CACGAG 1 cut(s) 227
Bst2BI CACGAG 1 cut(s) 227
Bst2UI CCWGG 1 cut(s) 101
Bst4CI ACNGT 4 cut(s) 276, 403, 467, 539
BstAPI GCANNNNNTGC 2 cut(s) 839, 860
BstBAI YACGTR 1 cut(s) 371
BstC8I GCNNGC 1 cut(s) 203
BstDEI CTNAG 1 cut(s) 197
BstDSI CCRYGG 1 cut(s) 281
BstENI CCTNNNNNAGG 1 cut(s) 206
BstF5I GGATG 5 cut(s) 519, 586, 643, 670, 796
BstFNI CGCG 4 cut(s) 14, 38, 341, 361
BstHHI GCGC 1 cut(s) 14
BstKTI GATC 7 cut(s) 20, 141, 220, 270, 339, 384, 487
BstMBI GATC 7 cut(s) 17, 138, 217, 267, 336, 381, 484
BstMCI CGRYCG 1 cut(s) 157
BstMWI GCNNNNNNNGC 9 cut(s) 35, 94, 303, 809, 818, 839, 860, 866, 890
BstNI CCWGG 1 cut(s) 101
BstSCI CCNGG 2 cut(s) 99, 156
BstSLI GKGCMC 2 cut(s) 196, 370
BstUI CGCG 4 cut(s) 14, 38, 341, 361
BstV1I GCAGC 6 cut(s) 150, 790, 805, 847, 871, 926
BstX2I RGATCY 1 cut(s) 17
BstYI RGATCY 1 cut(s) 17
BsuI GTATCC 1 cut(s) 416
BsuRI GGCC 4 cut(s) 194, 378, 625, 722
BtgI CCRYGG 1 cut(s) 281
BtsCI GGATG 5 cut(s) 519, 586, 643, 670, 796
Cac8I GCNNGC 1 cut(s) 203
CaiI CAGNNNCTG 1 cut(s) 957
CfoI GCGC 1 cut(s) 14
Cfr13I GGNCC 5 cut(s) 61, 192, 193, 377, 573
Csp6I GTAC 4 cut(s) 301, 562, 773, 949
CspCI CAANNNNNGTGG 2 cut(s) 215, 250
CviAII CATG 1 cut(s) 416
CviQI GTAC 4 cut(s) 301, 562, 773, 949
DdeI CTNAG 1 cut(s) 197
DpnI GATC 7 cut(s) 19, 140, 219, 269, 338, 383, 486
DpnII GATC 7 cut(s) 17, 138, 217, 267, 336, 381, 484
EciI GGCGGA 1 cut(s) 230
Eco24I GRGCYC 1 cut(s) 196
Eco47I GGWCC 2 cut(s) 61, 573
Eco57I CTGAAG 1 cut(s) 789
Eco72I CACGTG 1 cut(s) 371
EcoNI CCTNNNNNAGG 1 cut(s) 206
EcoO109I RGGNCCY 1 cut(s) 193
EcoRII CCWGG 1 cut(s) 99
EcoT38I GRGCYC 1 cut(s) 196
FaeI CATG 1 cut(s) 419
FaiI YATR 9 cut(s) 59, 417, 444, 474, 570, 621, 648, 657, 686
FalI AAGNNNNNCTT 2 cut(s) 826, 858
FatI CATG 1 cut(s) 415
FauI CCCGC 6 cut(s) 7, 31, 107, 290, 321, 354
FblI GTMKAC 1 cut(s) 43
Fnu4HI GCNGC 8 cut(s) 164, 307, 342, 804, 819, 861, 885, 940
FokI GGATG 5 cut(s) 526, 593, 650, 677, 803
FriOI GRGCYC 1 cut(s) 196
Fsp4HI GCNGC 8 cut(s) 164, 307, 342, 804, 819, 861, 885, 940
FspBI CTAG 3 cut(s) 9, 813, 970
GlaI GCGC 1 cut(s) 13
GluI GCNGC 8 cut(s) 164, 307, 342, 804, 819, 861, 885, 940
HaeIII GGCC 4 cut(s) 194, 378, 625, 722
HapII CCGG 2 cut(s) 157, 206
HhaI GCGC 1 cut(s) 14
Hin1II CATG 1 cut(s) 419
Hin6I GCGC 1 cut(s) 12
HinP1I GCGC 1 cut(s) 12
HincII GTYRAC 2 cut(s) 121, 151
HindII GTYRAC 2 cut(s) 121, 151
HinfI GANTC 3 cut(s) 131, 354, 602
HpaII CCGG 2 cut(s) 157, 206
HphI GGTGA 3 cut(s) 269, 376, 704
Hpy166II GTNNAC 5 cut(s) 44, 121, 151, 368, 460
Hpy188I TCNGA 4 cut(s) 55, 83, 136, 272
Hpy188III TCNNGA 3 cut(s) 221, 879, 903
Hpy8I GTNNAC 5 cut(s) 44, 121, 151, 368, 460
HpyAV CCTTC 6 cut(s) 258, 325, 537, 683, 745, 793
HpyCH4III ACNGT 4 cut(s) 276, 403, 467, 539
HpyCH4IV ACGT 1 cut(s) 370
HpyCH4V TGCA 5 cut(s) 97, 368, 833, 854, 942
HpyF10VI GCNNNNNNNGC 9 cut(s) 35, 94, 303, 809, 818, 839, 860, 866, 890
HpyF3I CTNAG 1 cut(s) 197
HpySE526I ACGT 1 cut(s) 370
Hsp92II CATG 1 cut(s) 419
HspAI GCGC 1 cut(s) 12
Kzo9I GATC 7 cut(s) 17, 138, 217, 267, 336, 381, 484
LmnI GCTCC 3 cut(s) 826, 892, 959
Lsp1109I GCAGC 6 cut(s) 150, 790, 805, 847, 871, 926
MaeI CTAG 3 cut(s) 9, 813, 970
MaeII ACGT 1 cut(s) 370
MaeIII GTNAC 5 cut(s) 221, 355, 371, 467, 492
MalI GATC 7 cut(s) 19, 140, 219, 269, 338, 383, 486
MboI GATC 7 cut(s) 17, 138, 217, 267, 336, 381, 484
MboII GAAGA 3 cut(s) 626, 692, 808
MflI RGATCY 1 cut(s) 17
MhlI GDGCHC 2 cut(s) 196, 370
MluCI AATT 2 cut(s) 439, 695
MlyI GAGTC 1 cut(s) 363
MmeI TCCRAC 2 cut(s) 300, 848
MroXI GAANNNNTTC 1 cut(s) 841
MseI TTAA 4 cut(s) 405, 525, 743, 872
MspA1I CMGCKG 1 cut(s) 316
MspI CCGG 2 cut(s) 157, 206
MspR9I CCNGG 2 cut(s) 101, 158
MvaI CCWGG 1 cut(s) 101
MvnI CGCG 4 cut(s) 14, 38, 341, 361
MwoI GCNNNNNNNGC 9 cut(s) 35, 94, 303, 809, 818, 839, 860, 866, 890
NciI CCSGG 1 cut(s) 158
NdeII GATC 7 cut(s) 17, 138, 217, 267, 336, 381, 484
NlaIII CATG 1 cut(s) 419
NlaIV GGNNCC 2 cut(s) 19, 194
NmuCI GTSAC 5 cut(s) 221, 355, 371, 467, 492
PdmI GAANNNNTTC 1 cut(s) 841
PfeI GAWTC 2 cut(s) 131, 602
PkrI GCNGC 8 cut(s) 165, 308, 343, 805, 820, 862, 886, 941
PleI GAGTC 1 cut(s) 362
PmaCI CACGTG 1 cut(s) 371
PmlI CACGTG 1 cut(s) 371
PpsI GAGTC 1 cut(s) 362
Ppu21I YACGTR 1 cut(s) 371
Psp6I CCWGG 1 cut(s) 99
PspCI CACGTG 1 cut(s) 371
PspGI CCWGG 1 cut(s) 99
PspN4I GGNNCC 2 cut(s) 19, 194
PspOMI GGGCCC 1 cut(s) 192
PspPI GGNCC 5 cut(s) 61, 192, 193, 377, 573
PsrI GAACNNNNNNTAC 2 cut(s) 829, 861
PstNI CAGNNNCTG 1 cut(s) 957
PsuI RGATCY 1 cut(s) 17
RsaI GTAC 4 cut(s) 302, 563, 774, 950
RsaNI GTAC 4 cut(s) 301, 562, 773, 949
SaqAI TTAA 4 cut(s) 405, 525, 743, 872
SatI GCNGC 8 cut(s) 164, 307, 342, 804, 819, 861, 885, 940
Sau3AI GATC 7 cut(s) 17, 138, 217, 267, 336, 381, 484
Sau96I GGNCC 5 cut(s) 61, 192, 193, 377, 573
ScaI AGTACT 1 cut(s) 774
SchI GAGTC 1 cut(s) 363
ScrFI CCNGG 2 cut(s) 101, 158
SduI GDGCHC 2 cut(s) 196, 370
SinI GGWCC 2 cut(s) 61, 573
SmlI CTYRAG 2 cut(s) 877, 901
SmoI CTYRAG 2 cut(s) 877, 901
Sse9I AATT 2 cut(s) 439, 695
SspMI CTAG 3 cut(s) 9, 813, 970
StyD4I CCNGG 2 cut(s) 99, 156
TaaI ACNGT 4 cut(s) 276, 403, 467, 539
TaiI ACGT 1 cut(s) 373
TaqI TCGA 3 cut(s) 487, 519, 927
TaqII GACCGA 1 cut(s) 590
TasI AATT 2 cut(s) 439, 695
TatI WGTACW 1 cut(s) 772
TauI GCSGC 2 cut(s) 309, 344
TfiI GAWTC 2 cut(s) 131, 602
Tru1I TTAA 4 cut(s) 405, 525, 743, 872
Tru9I TTAA 4 cut(s) 405, 525, 743, 872
TseFI GTSAC 5 cut(s) 221, 355, 371, 467, 492
TseI GCWGC 6 cut(s) 163, 803, 818, 860, 884, 939
Tsp45I GTSAC 5 cut(s) 221, 355, 371, 467, 492
TspDTI ATGAA 2 cut(s) 600, 627
TspGWI ACGGA 1 cut(s) 606
VneI GTGCAC 1 cut(s) 366
VpaK11BI GGWCC 2 cut(s) 61, 573
XagI CCTNNNNNAGG 1 cut(s) 206
XapI RAATTY 1 cut(s) 695
XmiI GTMKAC 1 cut(s) 43
XmnI GAANNNNTTC 1 cut(s) 841
XspI CTAG 3 cut(s) 9, 813, 970
ZrmI AGTACT 1 cut(s) 774
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.