MD02G1198400.v1.1

carboxylesterase 8

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0005485
Physical Location & Seq
Reverse (-)
19480577 .. 19481593
1017 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1198400.v1.1.491

Sequence Viewer

Length: 1017 bp
ATGGCGGATGAACCTTCTTCTCCGAACCCGGCAGCCTCCATCGACCTCTACAAATTCATCCTCCCAAACCCAGACGGCTCCCTCAACCGCGCCACCCCATTATTCCCCATTGTCCCCCCAACTCTGACTCCACCAGCTGAGTCCTTTCGCACCAAGTCAAACTCCAACACGCCCCAACTCGTCCTCTCCAAGGACATTCCCTTAAACCCAGAAACCAAAACCTTCCTGAGGCTCTTCAAACCCCACCCACTCCCCCCAAACCCCCACCTCGCTCTCATCCTCTACTTCCACGGCGGCGGCTTCGTCCTCTTCTCCGCCGCCTCCAAACCCTACCACGACACCTGTTCCGAAATGGCTCTTTCCCTCCGAGCCATAATCGTCTCCGTCGACTACCGTTTAGCTCCTGAACACCCTCTCCCGTCTGCATTCGATGACGCCGTCGAAGCCATCGCCTGGGCCCGCAGCCAGGCCTCCGACGTCGATGGCCGCGATCCCTGGCTGAAAGACGCGGTCGACTTCTCCAAGTGCTTTTTAATGGGCAGCAGCGCCGGGGGAACTATGGTCTACCATGCGGGTGTACGCGTCTCCGATGTCGATCTCTCGCCGTTGACGATCCGAGGGCTGATATTCAACCAGCCTTATTTCGGAGGGGTCCAGAGGACTCAATCTGAGTTGAAGCTGATCGACGATCAGGTTCTACCGTTGGTCACCAGTGACATGATGTGGGGCCATGCTTTGCCGAAGGGAGTGGATCTGGACCACGAGTATTGTAATCCGACGGTGAGAGGTGGGGACAGGAGGATGCGACGGTTGCCCAAGTGCTTGGTTAGAGGGAACGGCGGGGACCCACTTCTGGATAGGCAGAGGGAGTTTGCGGCATTGCTGGAGTCACGTGGGGTGCACGTGGTGTCCAAATTTGACGAGGGTGGTTGTCATGCTGTGGAGCTCTTTGATCCCGGCATGGCCCAAGTTCTGTACGACATTATTGGGGATTTTATAGAGTGCTGCTGTGAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0009056 GO:0016787
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

339

Amino Acids

37.13

Weight (kDa)

5.47

Isoelectric Point (pI)

36.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BD-FAE PF20434 90 - 196 1.1e-09 BD-FAE
Abhydrolase_3 PF07859 93 - 314 1.3e-52 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 480
AccB7I CCANNNNNTGG 1 cut(s) 453
AccI GTMKAC 3 cut(s) 387, 513, 564
AccII CGCG 4 cut(s) 90, 489, 509, 582
AclWI GGATC 4 cut(s) 485, 607, 759, 947
AcoI YGGCCR 1 cut(s) 484
AcsI RAATTY 2 cut(s) 53, 914
AcvI CACGTG 2 cut(s) 893, 904
AcyI GRCGYC 2 cut(s) 435, 477
AdeI CACNNNGTG 1 cut(s) 907
AfaI GTAC 2 cut(s) 579, 977
AfiI CCNNNNNNNGG 6 cut(s) 190, 228, 453, 466, 644, 853
AflIII ACRYGT 1 cut(s) 580
AgsI TTSAA 3 cut(s) 238, 631, 676
AjnI CCWGG 3 cut(s) 452, 465, 494
AloI GAACNNNNNNTCC 2 cut(s) 399, 431
AluBI AGCT 4 cut(s) 137, 401, 679, 946
AluI AGCT 4 cut(s) 137, 401, 679, 946
Alw21I GWGCWC 2 cut(s) 903, 948
Alw26I GTCTC 2 cut(s) 385, 589
Alw44I GTGCAC 1 cut(s) 899
AlwI GGATC 4 cut(s) 485, 607, 759, 947
AoxI GGCC 5 cut(s) 456, 468, 484, 727, 963
ApaI GGGCCC 1 cut(s) 460
ApaLI GTGCAC 1 cut(s) 899
ApeKI GCWGC 5 cut(s) 32, 462, 540, 543, 1005
ApoI RAATTY 2 cut(s) 53, 914
AspLEI GCGC 2 cut(s) 92, 548
AspS9I GGNCC 7 cut(s) 456, 457, 652, 727, 757, 844, 964
AsuC2I CCSGG 3 cut(s) 29, 550, 957
AsuHPI GGTGA 2 cut(s) 700, 793
AvaII GGWCC 3 cut(s) 652, 757, 844
AxyI CCTNAGG 1 cut(s) 227
BaeGI GKGCMC 2 cut(s) 460, 903
BanII GRGCYC 2 cut(s) 460, 948
BauI CACGAG 1 cut(s) 761
BbrPI CACGTG 2 cut(s) 893, 904
Bbv12I GWGCWC 2 cut(s) 903, 948
BbvI GCAGC 5 cut(s) 44, 474, 552, 555, 992
BccI CCATC 3 cut(s) 47, 455, 476
BceAI ACGGC 5 cut(s) 91, 307, 422, 589, 853
BciT130I CCWGG 3 cut(s) 454, 467, 496
BcnI CCSGG 3 cut(s) 29, 550, 957
BcoDI GTCTC 2 cut(s) 385, 589
BfoI RGCGCY 1 cut(s) 549
Bme1390I CCNGG 6 cut(s) 29, 454, 467, 496, 550, 957
Bme18I GGWCC 3 cut(s) 652, 757, 844
BmgT120I GGNCC 7 cut(s) 456, 457, 652, 727, 757, 844, 964
BmiI GGNNCC 6 cut(s) 79, 458, 653, 728, 845, 846
BmrFI CCNGG 6 cut(s) 29, 454, 467, 496, 550, 957
BmsI GCATC 1 cut(s) 792
BpmI CTGGAG 1 cut(s) 905
BpuMI CCSGG 3 cut(s) 29, 550, 957
BsaAI YACGTR 2 cut(s) 893, 904
BsaBI GATNNNNATC 1 cut(s) 594
BsaHI GRCGYC 2 cut(s) 435, 477
BsaJI CCNNGG 6 cut(s) 189, 289, 453, 494, 549, 616
BsaXI ACNNNNNCTCC 4 cut(s) 112, 142, 399, 429
Bsc4I CCNNNNNNNGG 6 cut(s) 190, 228, 453, 466, 644, 853
Bse1I ACTGG 1 cut(s) 711
Bse21I CCTNAGG 1 cut(s) 227
Bse3DI GCAATG 1 cut(s) 878
Bse8I GATNNNNATC 1 cut(s) 594
BseBI CCWGG 3 cut(s) 454, 467, 496
BseDI CCNNGG 6 cut(s) 189, 289, 453, 494, 549, 616
BseGI GGATG 4 cut(s) 13, 57, 276, 807
BseJI GATNNNNATC 1 cut(s) 594
BseLI CCNNNNNNNGG 6 cut(s) 190, 228, 453, 466, 644, 853
BseMI GCAATG 1 cut(s) 878
BseMII CTCAG 3 cut(s) 129, 218, 660
BseNI ACTGG 1 cut(s) 711
BseSI GKGCMC 2 cut(s) 460, 903
BseXI GCAGC 5 cut(s) 44, 474, 552, 555, 992
Bsh1236I CGCG 4 cut(s) 90, 489, 509, 582
Bsh1285I CGRYCG 1 cut(s) 513
BshFI GGCC 5 cut(s) 458, 470, 486, 729, 965
BsiEI CGRYCG 1 cut(s) 513
BsiHKAI GWGCWC 2 cut(s) 903, 948
BsiSI CCGG 3 cut(s) 29, 549, 957
BslFI GGGAC 3 cut(s) 98, 806, 857
BslI CCNNNNNNNGG 6 cut(s) 190, 228, 453, 466, 644, 853
BsmAI GTCTC 2 cut(s) 385, 589
BsmBI CGTCTC 2 cut(s) 385, 589
BsmFI GGGAC 3 cut(s) 98, 806, 857
BsmI GAATGC 1 cut(s) 425
BsnI GGCC 5 cut(s) 458, 470, 486, 729, 965
Bsp120I GGGCCC 1 cut(s) 456
Bsp1286I GDGCHC 3 cut(s) 460, 903, 948
Bsp143I GATC 7 cut(s) 490, 595, 612, 681, 688, 751, 952
BspANI GGCC 5 cut(s) 458, 470, 486, 729, 965
BspCNI CTCAG 3 cut(s) 130, 219, 661
BspFNI CGCG 4 cut(s) 90, 489, 509, 582
BspLI GGNNCC 6 cut(s) 79, 458, 653, 728, 845, 846
BspPI GGATC 4 cut(s) 485, 607, 759, 947
BspQI GCTCTTC 1 cut(s) 239
BsrDI GCAATG 1 cut(s) 878
BsrI ACTGG 1 cut(s) 711
BssECI CCNNGG 6 cut(s) 189, 289, 453, 494, 549, 616
BssMI GATC 7 cut(s) 490, 595, 612, 681, 688, 751, 952
BssNI GRCGYC 2 cut(s) 435, 477
BssSI CACGAG 1 cut(s) 761
BssT1I CCWWGG 1 cut(s) 189
Bst2BI CACGAG 1 cut(s) 761
Bst2UI CCWGG 3 cut(s) 454, 467, 496
Bst4CI ACNGT 4 cut(s) 395, 702, 781, 810
Bst6I CTCTTC 2 cut(s) 239, 314
BstACI GRCGYC 2 cut(s) 435, 477
BstBAI YACGTR 2 cut(s) 893, 904
BstC8I GCNNGC 1 cut(s) 460
BstDEI CTNAG 3 cut(s) 138, 227, 669
BstDSI CCRYGG 1 cut(s) 289
BstEII GGTNACC 1 cut(s) 706
BstENI CCTNNNNNAGG 2 cut(s) 188, 226
BstF5I GGATG 4 cut(s) 13, 57, 276, 807
BstFNI CGCG 4 cut(s) 90, 489, 509, 582
BstH2I RGCGCY 1 cut(s) 549
BstHHI GCGC 2 cut(s) 92, 548
BstKTI GATC 7 cut(s) 493, 598, 615, 684, 691, 754, 955
BstMAI GTCTC 2 cut(s) 385, 589
BstMBI GATC 7 cut(s) 490, 595, 612, 681, 688, 751, 952
BstMCI CGRYCG 1 cut(s) 513
BstMWI GCNNNNNNNGC 2 cut(s) 443, 811
BstNI CCWGG 3 cut(s) 454, 467, 496
BstPI GGTNACC 1 cut(s) 706
BstSCI CCNGG 6 cut(s) 27, 452, 465, 494, 548, 955
BstSLI GKGCMC 2 cut(s) 460, 903
BstUI CGCG 4 cut(s) 90, 489, 509, 582
BstV1I GCAGC 5 cut(s) 44, 474, 552, 555, 992
BstX2I RGATCY 1 cut(s) 751
BstXI CCANNNNNNTGG 1 cut(s) 823
BstYI RGATCY 1 cut(s) 751
Bsu36I CCTNAGG 1 cut(s) 227
BsuRI GGCC 5 cut(s) 458, 470, 486, 729, 965
BtgI CCRYGG 1 cut(s) 289
BtgZI GCGATG 1 cut(s) 433
BtsCI GGATG 4 cut(s) 13, 57, 276, 807
BtsIMutI CAGTG 1 cut(s) 718
Cac8I GCNNGC 1 cut(s) 460
CfoI GCGC 2 cut(s) 92, 548
Cfr13I GGNCC 7 cut(s) 456, 457, 652, 727, 757, 844, 964
CseI GACGC 3 cut(s) 443, 515, 571
Csp6I GTAC 2 cut(s) 578, 976
CspCI CAANNNNNGTGG 2 cut(s) 749, 784
CviAII CATG 5 cut(s) 569, 718, 731, 935, 961
CviQI GTAC 2 cut(s) 578, 976
DdeI CTNAG 3 cut(s) 138, 227, 669
DpnI GATC 7 cut(s) 492, 597, 614, 683, 690, 753, 954
DpnII GATC 7 cut(s) 490, 595, 612, 681, 688, 751, 952
DraIII CACNNNGTG 1 cut(s) 907
EaeI YGGCCR 1 cut(s) 484
Eam1104I CTCTTC 2 cut(s) 239, 314
EarI CTCTTC 2 cut(s) 239, 314
EciI GGCGGA 2 cut(s) 20, 304
Ecl136II GAGCTC 1 cut(s) 946
Eco130I CCWWGG 1 cut(s) 189
Eco147I AGGCCT 1 cut(s) 470
Eco24I GRGCYC 2 cut(s) 460, 948
Eco47I GGWCC 3 cut(s) 652, 757, 844
Eco53kI GAGCTC 1 cut(s) 946
Eco72I CACGTG 2 cut(s) 893, 904
Eco81I CCTNAGG 1 cut(s) 227
Eco91I GGTNACC 1 cut(s) 706
EcoICRI GAGCTC 1 cut(s) 946
EcoNI CCTNNNNNAGG 2 cut(s) 188, 226
EcoO109I RGGNCCY 1 cut(s) 844
EcoO65I GGTNACC 1 cut(s) 706
EcoRII CCWGG 3 cut(s) 452, 465, 494
EcoT14I CCWWGG 1 cut(s) 189
EcoT38I GRGCYC 2 cut(s) 460, 948
ErhI CCWWGG 1 cut(s) 189
Esp3I CGTCTC 2 cut(s) 385, 589
FaeI CATG 5 cut(s) 572, 721, 734, 938, 964
FaiI YATR 8 cut(s) 374, 560, 570, 719, 732, 936, 962, 998
FaqI GGGAC 3 cut(s) 98, 806, 857
FatI CATG 5 cut(s) 568, 717, 730, 934, 960
FauI CCCGC 3 cut(s) 467, 565, 833
FblI GTMKAC 3 cut(s) 387, 513, 564
FokI GGATG 4 cut(s) 20, 44, 263, 814
FriOI GRGCYC 2 cut(s) 460, 948
GlaI GCGC 2 cut(s) 91, 547
GsuI CTGGAG 1 cut(s) 905
HaeII RGCGCY 1 cut(s) 549
HaeIII GGCC 5 cut(s) 458, 470, 486, 729, 965
HapII CCGG 3 cut(s) 29, 549, 957
HgaI GACGC 3 cut(s) 443, 515, 571
HhaI GCGC 2 cut(s) 92, 548
Hin1I GRCGYC 2 cut(s) 435, 477
Hin1II CATG 5 cut(s) 572, 721, 734, 938, 964
Hin6I GCGC 2 cut(s) 90, 546
HinP1I GCGC 2 cut(s) 90, 546
HincII GTYRAC 3 cut(s) 388, 514, 609
HindII GTYRAC 3 cut(s) 388, 514, 609
HinfI GANTC 4 cut(s) 127, 140, 661, 887
HpaII CCGG 3 cut(s) 29, 549, 957
HphI GGTGA 2 cut(s) 700, 793
Hpy166II GTNNAC 6 cut(s) 388, 514, 565, 578, 609, 901
Hpy188III TCNNGA 5 cut(s) 226, 404, 655, 755, 854
Hpy8I GTNNAC 6 cut(s) 388, 514, 565, 578, 609, 901
Hpy99I CGWCG 7 cut(s) 389, 443, 479, 482, 689, 781, 810
HpyAV CCTTC 3 cut(s) 24, 232, 736
HpyCH4III ACNGT 4 cut(s) 395, 702, 781, 810
HpyCH4IV ACGT 3 cut(s) 477, 892, 903
HpyCH4V TGCA 2 cut(s) 425, 901
HpyF10VI GCNNNNNNNGC 2 cut(s) 443, 811
HpyF3I CTNAG 3 cut(s) 138, 227, 669
HpySE526I ACGT 3 cut(s) 477, 892, 903
Hsp92I GRCGYC 2 cut(s) 435, 477
Hsp92II CATG 5 cut(s) 572, 721, 734, 938, 964
HspAI GCGC 2 cut(s) 90, 546
KflI GGGWCCC 1 cut(s) 844
Kzo9I GATC 7 cut(s) 490, 595, 612, 681, 688, 751, 952
LguI GCTCTTC 1 cut(s) 239
LmnI GCTCC 3 cut(s) 83, 406, 943
Lsp1109I GCAGC 5 cut(s) 44, 474, 552, 555, 992
LweI GCATC 1 cut(s) 792
MaeII ACGT 3 cut(s) 477, 892, 903
MaeIII GTNAC 3 cut(s) 706, 713, 888
MalI GATC 7 cut(s) 492, 597, 614, 683, 690, 753, 954
MboI GATC 7 cut(s) 490, 595, 612, 681, 688, 751, 952
MboII GAAGA 3 cut(s) 9, 226, 301
MflI RGATCY 1 cut(s) 751
MhlI GDGCHC 3 cut(s) 460, 903, 948
MluCI AATT 2 cut(s) 53, 914
MluI ACGCGT 1 cut(s) 580
MlyI GAGTC 4 cut(s) 121, 149, 655, 896
MmeI TCCRAC 3 cut(s) 189, 498, 800
MseI TTAA 2 cut(s) 203, 533
MslI CAYNNNNRTG 1 cut(s) 573
MspA1I CMGCKG 1 cut(s) 137
MspI CCGG 3 cut(s) 29, 549, 957
MspR9I CCNGG 6 cut(s) 29, 454, 467, 496, 550, 957
Mva1269I GAATGC 1 cut(s) 425
MvaI CCWGG 3 cut(s) 454, 467, 496
MvnI CGCG 4 cut(s) 90, 489, 509, 582
MwoI GCNNNNNNNGC 2 cut(s) 443, 811
NciI CCSGG 3 cut(s) 29, 550, 957
NdeII GATC 7 cut(s) 490, 595, 612, 681, 688, 751, 952
NlaIII CATG 5 cut(s) 572, 721, 734, 938, 964
NlaIV GGNNCC 6 cut(s) 79, 458, 653, 728, 845, 846
NmuCI GTSAC 3 cut(s) 706, 713, 888
PceI AGGCCT 1 cut(s) 470
PciSI GCTCTTC 1 cut(s) 239
PcsI WCGNNNNNNNCGW 4 cut(s) 384, 435, 486, 608
PctI GAATGC 1 cut(s) 425
PflFI GACNNNGTC 2 cut(s) 437, 509
PflMI CCANNNNNTGG 1 cut(s) 453
PleI GAGTC 4 cut(s) 121, 148, 655, 895
PmaCI CACGTG 2 cut(s) 893, 904
PmlI CACGTG 2 cut(s) 893, 904
PpsI GAGTC 4 cut(s) 121, 148, 655, 895
Ppu21I YACGTR 2 cut(s) 893, 904
PpuMI RGGWCCY 1 cut(s) 844
Psp124BI GAGCTC 1 cut(s) 948
Psp5II RGGWCCY 1 cut(s) 844
Psp6I CCWGG 3 cut(s) 452, 465, 494
PspCI CACGTG 2 cut(s) 893, 904
PspEI GGTNACC 1 cut(s) 706
PspGI CCWGG 3 cut(s) 452, 465, 494
PspN4I GGNNCC 6 cut(s) 79, 458, 653, 728, 845, 846
PspOMI GGGCCC 1 cut(s) 456
PspPI GGNCC 7 cut(s) 456, 457, 652, 727, 757, 844, 964
PspPPI RGGWCCY 1 cut(s) 844
PsuI RGATCY 1 cut(s) 751
PsyI GACNNNGTC 2 cut(s) 437, 509
PvuII CAGCTG 1 cut(s) 137
RsaI GTAC 2 cut(s) 579, 977
RsaNI GTAC 2 cut(s) 578, 976
RseI CAYNNNNRTG 1 cut(s) 573
SacI GAGCTC 1 cut(s) 948
SalI GTCGAC 2 cut(s) 386, 512
SapI GCTCTTC 1 cut(s) 239
SaqAI TTAA 2 cut(s) 203, 533
Sau3AI GATC 7 cut(s) 490, 595, 612, 681, 688, 751, 952
Sau96I GGNCC 7 cut(s) 456, 457, 652, 727, 757, 844, 964
SchI GAGTC 4 cut(s) 121, 149, 655, 896
ScrFI CCNGG 6 cut(s) 29, 454, 467, 496, 550, 957
SduI GDGCHC 3 cut(s) 460, 903, 948
SfaNI GCATC 1 cut(s) 792
SinI GGWCC 3 cut(s) 652, 757, 844
SmiMI CAYNNNNRTG 1 cut(s) 573
Sse9I AATT 2 cut(s) 53, 914
SseBI AGGCCT 1 cut(s) 470
SstI GAGCTC 1 cut(s) 948
StuI AGGCCT 1 cut(s) 470
StyD4I CCNGG 6 cut(s) 27, 452, 465, 494, 548, 955
StyI CCWWGG 1 cut(s) 189
TaaI ACNGT 4 cut(s) 395, 702, 781, 810
TaiI ACGT 3 cut(s) 480, 895, 906
TaqI TCGA 8 cut(s) 42, 387, 429, 441, 480, 513, 594, 684
TasI AATT 2 cut(s) 53, 914
TauI GCSGC 5 cut(s) 297, 300, 320, 489, 878
Tru1I TTAA 2 cut(s) 203, 533
Tru9I TTAA 2 cut(s) 203, 533
TscAI CASTG 1 cut(s) 718
TseFI GTSAC 3 cut(s) 706, 713, 888
TseI GCWGC 5 cut(s) 32, 462, 540, 543, 1005
Tsp45I GTSAC 3 cut(s) 706, 713, 888
TspDTI ATGAA 2 cut(s) 24, 46
TspGWI ACGGA 1 cut(s) 373
TspRI CASTG 1 cut(s) 718
Tth111I GACNNNGTC 2 cut(s) 437, 509
Van91I CCANNNNNTGG 1 cut(s) 453
VneI GTGCAC 1 cut(s) 899
VpaK11BI GGWCC 3 cut(s) 652, 757, 844
XagI CCTNNNNNAGG 2 cut(s) 188, 226
XapI RAATTY 2 cut(s) 53, 914
XmiI GTMKAC 3 cut(s) 387, 513, 564
ZraI GACGTC 1 cut(s) 478
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.