Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0005485

Physical Location & Seq

Genomic Coordinates Reverse (-)

19495017 .. 19496075

Gene Structure 1059 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1198500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1059 bp

ATGGCGTATCAACCTTCTTCATCCCCAACCTCCATTGACCCCTACAAACTCCTCAAAATCCTCCCCAACCCTGATGGCTCCCTCACCCGCTTCACCCCTTTCCCCACCGTCTCCCCAACTCCCACCCCCACCGAGTCCATTTCCGCCACGGCCGCCGAGTCAGACTCCAACTCTCCCCAACTCGTCCTCTCCAAGGACATTCCCTTAAACCCAGAAACAAAAACCTTCTTCAGGCTCTTCAAACCCCACCCTCTCCCCCCAAACCCCCGCCTCCCTCTCATCATCTACTTCCACGGAGGCGGCTTCGTCCTCTTCTCCGCCGCCTCCAAACCATTCCACGACTCCTGCTCTCGCATGGCCCTGGCTCTCCAAGCCCTAATTGTCTCCGTCGAGTACCGCTTGGCTCCCGAACATCCTCTTCCTTCAGCCTACGATGACGCCGTCGACACCATCACCTGGGTCCACCGCCAGGCCTCCGACGTCGACGGCTGCGATCCCTGGTTGAAAGACGCCGTTGACTTCTCCAGGTCCTTCCTAATGGGCAGCAGCGCCGGAGCTAACATCGCCTACCACGCGGGTTTACGCGTCTCGGGTGATGATCTCTTGCCGATGAAGATCCGAGGGCTGATTCTAAACCAGCCTTATTTCGGCGGGGTCCATAGGACTGAATCCGAGACGAGGTTGATCAACGACCGGATATTGCCGCTGGTCGCCAACGACTTGATGTGGGGCATGGCTTTGCCAAAGGGTGTTGATCGGGATCACGAGTATTGTAATCCGACGGCGGGAGGTGGGGATGGGAGGATCGAACGGTTGCCGAGGTGTTTGGTAAGAGGGTACGGGGGGGACCCGCTGATGGATAGGCAGAAGGAGTTTGTGGCGTTACTTGAGTCACGTGGGGCGCACGTGGTGGCCAAGTTTGACGAGGGGGGTTTTCATGGCGTGGAGCTCTTCGACCCTAGCAAGGCCCAAGCTCTGTACGACATTCTTAGGGATTTTATCGAGTCGTGCGATGCATACGACGGTGATAATATTGTCGTTTCAAAGTCGGCGATGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0009056 GO:0016787

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Abhydrolase_3

Protein Analysis

353

Amino Acids

38.59

Weight (kDa)

5.46

Isoelectric Point (pI)

44.28

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
BD-FAE	PF20434	90 - 196	1.3e-10	BD-FAE
Abhydrolase_3	PF07859	94 - 315	5e-52	alpha/beta hydrolase fold

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005485)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G45600
fragaria_vesca	FvH4_7g12500
malus_domestica	MD02G1198400.v1.1 MD02G1198500.v1.1 MD02G1198600.v1.1
prunus_persica	Prupe.2G149600_v2.0.a1
pyrus_communis	pycom02g16210
rosa_chinensis	RchiOBHm_Chr1g0351371 RchiOBHm_Chr1g0351401
rosa_laevigata	RLG00000028409 RLG00000028410
rosa_multiflora	Rmu_sc0000664.1_g000003 Rmu_sc0000664.1_g000005 Rmu_sc0000664.1_g000006 Rmu_sc0005320.1_g000006 Rmu_sc0005320.1_g000011
rosa_roxburghii	Rroxscaffold_4G00303850
rosa_rugosa	Rorug01G0217700 Rorug01G0217900
rosa_samantha	Rh1AG232300 Rh1AG232400 Rh1BG202700 Rh1BG203200 Rh1CG217100 Rh1DG229800 Rh1DG230200
rosa_wichuraiana	Rw0G011320 Rw1G020230 Rw1G020240

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	483
AccB7I	CCANNNNNTGG	1 cut(s)	456
AccI	GTMKAC	2 cut(s)	444, 483
AccII	CGCG	2 cut(s)	575, 585
AclWI	GGATC	4 cut(s)	488, 610, 768, 812
AcoI	YGGCCR	2 cut(s)	150, 912
AcuI	CTGAAG	2 cut(s)	214, 408
AcvI	CACGTG	2 cut(s)	896, 907
AcyI	GRCGYC	3 cut(s)	438, 480, 510
AdeI	CACNNNGTG	1 cut(s)	910
AfaI	GTAC	3 cut(s)	395, 839, 980
AfiI	CCNNNNNNNGG	9 cut(s)	193, 231, 456, 469, 647, 678, 785, 856, 964
AflIII	ACRYGT	1 cut(s)	583
AgsI	TTSAA	3 cut(s)	241, 505, 1044
AjnI	CCWGG	5 cut(s)	360, 455, 468, 497, 524
AluBI	AGCT	3 cut(s)	557, 949, 974
AluI	AGCT	3 cut(s)	557, 949, 974
Alw21I	GWGCWC	1 cut(s)	951
Alw26I	GTCTC	4 cut(s)	115, 388, 592, 668
AlwI	GGATC	4 cut(s)	488, 610, 768, 812
Ama87I	CYCGRG	1 cut(s)	589
AoxI	GGCC	5 cut(s)	150, 357, 471, 912, 966
ApeKI	GCWGC	3 cut(s)	489, 543, 546
AspLEI	GCGC	2 cut(s)	551, 904
AspS9I	GGNCC	6 cut(s)	358, 460, 528, 655, 847, 967
AsuHPI	GGTGA	5 cut(s)	76, 85, 445, 605, 1037
AvaI	CYCGRG	1 cut(s)	589
AvaII	GGWCC	4 cut(s)	460, 528, 655, 847
BalI	TGGCCA	1 cut(s)	914
BanII	GRGCYC	1 cut(s)	951
BauI	CACGAG	1 cut(s)	764
BbrPI	CACGTG	2 cut(s)	896, 907
Bbv12I	GWGCWC	1 cut(s)	951
BbvI	GCAGC	3 cut(s)	476, 555, 558
BccI	CCATC	4 cut(s)	68, 458, 791, 850
BceAI	ACGGC	5 cut(s)	165, 425, 497, 502, 798
BciT130I	CCWGG	5 cut(s)	362, 457, 470, 499, 526
BclI	TGATCA	1 cut(s)	684
BcoDI	GTCTC	4 cut(s)	115, 388, 592, 668
BfaI	CTAG	1 cut(s)	960
BfoI	RGCGCY	1 cut(s)	552
BisI	GCNGC	7 cut(s)	153, 301, 321, 490, 544, 547, 704
BlsI	GCNGC	7 cut(s)	154, 302, 322, 491, 545, 548, 705
Bme1390I	CCNGG	5 cut(s)	362, 457, 470, 499, 526
Bme18I	GGWCC	4 cut(s)	460, 528, 655, 847
BmeT110I	CYCGRG	1 cut(s)	589
BmgT120I	GGNCC	6 cut(s)	358, 460, 528, 655, 847, 967
BmiI	GGNNCC	6 cut(s)	79, 405, 461, 656, 848, 849
BmrFI	CCNGG	5 cut(s)	362, 457, 470, 499, 526
BmsI	GCATC	1 cut(s)	1003
BplI	GAGNNNNNCTC	2 cut(s)	149, 181
BpmI	CTGGAG	1 cut(s)	508
BpuEI	CTTGAG	1 cut(s)	908
BsaAI	YACGTR	2 cut(s)	896, 907
BsaBI	GATNNNNATC	2 cut(s)	614, 759
BsaHI	GRCGYC	3 cut(s)	438, 480, 510
BsaJI	CCNNGG	8 cut(s)	147, 192, 292, 360, 456, 497, 619, 818
BsaWI	WCCGGW	1 cut(s)	693
Bsc4I	CCNNNNNNNGG	9 cut(s)	193, 231, 456, 469, 647, 678, 785, 856, 964
Bse8I	GATNNNNATC	2 cut(s)	614, 759
BseBI	CCWGG	5 cut(s)	362, 457, 470, 499, 526
BseDI	CCNNGG	8 cut(s)	147, 192, 292, 360, 456, 497, 619, 818
BseGI	GGATG	3 cut(s)	20, 412, 802
BseJI	GATNNNNATC	2 cut(s)	614, 759
BseLI	CCNNNNNNNGG	9 cut(s)	193, 231, 456, 469, 647, 678, 785, 856, 964
BseRI	GAGGAG	1 cut(s)	41
BseX3I	CGGCCG	1 cut(s)	150
BseXI	GCAGC	3 cut(s)	476, 555, 558
Bsh1236I	CGCG	2 cut(s)	575, 585
Bsh1285I	CGRYCG	2 cut(s)	153, 694
BshFI	GGCC	5 cut(s)	152, 359, 473, 914, 968
BsiEI	CGRYCG	2 cut(s)	153, 694
BsiHKAI	GWGCWC	1 cut(s)	951
BsiHKCI	CYCGRG	1 cut(s)	589
BsiSI	CCGG	2 cut(s)	552, 694
BslFI	GGGAC	1 cut(s)	860
BslI	CCNNNNNNNGG	9 cut(s)	193, 231, 456, 469, 647, 678, 785, 856, 964
BsmAI	GTCTC	4 cut(s)	115, 388, 592, 668
BsmBI	CGTCTC	3 cut(s)	115, 592, 668
BsmFI	GGGAC	1 cut(s)	860
BsnI	GGCC	5 cut(s)	152, 359, 473, 914, 968
BsoBI	CYCGRG	1 cut(s)	589
Bsp1286I	GDGCHC	1 cut(s)	951
Bsp143I	GATC	7 cut(s)	493, 598, 615, 684, 754, 760, 804
BspANI	GGCC	5 cut(s)	152, 359, 473, 914, 968
BspFNI	CGCG	2 cut(s)	575, 585
BspLI	GGNNCC	6 cut(s)	79, 405, 461, 656, 848, 849
BspPI	GGATC	4 cut(s)	488, 610, 768, 812
BspQI	GCTCTTC	2 cut(s)	242, 956
BssECI	CCNNGG	8 cut(s)	147, 192, 292, 360, 456, 497, 619, 818
BssMI	GATC	7 cut(s)	493, 598, 615, 684, 754, 760, 804
BssNI	GRCGYC	3 cut(s)	438, 480, 510
BssSI	CACGAG	1 cut(s)	764
BssT1I	CCWWGG	1 cut(s)	192
Bst2BI	CACGAG	1 cut(s)	764
Bst2UI	CCWGG	5 cut(s)	362, 457, 470, 499, 526
Bst4CI	ACNGT	3 cut(s)	109, 813, 1025
Bst6I	CTCTTC	4 cut(s)	242, 317, 423, 956
BstACI	GRCGYC	3 cut(s)	438, 480, 510
BstBAI	YACGTR	2 cut(s)	896, 907
BstDEI	CTNAG	1 cut(s)	989
BstDSI	CCRYGG	2 cut(s)	147, 292
BstENI	CCTNNNNNAGG	2 cut(s)	191, 229
BstF5I	GGATG	3 cut(s)	20, 412, 802
BstFNI	CGCG	2 cut(s)	575, 585
BstH2I	RGCGCY	1 cut(s)	552
BstHHI	GCGC	2 cut(s)	551, 904
BstKTI	GATC	7 cut(s)	496, 601, 618, 687, 757, 763, 807
BstMAI	GTCTC	4 cut(s)	115, 388, 592, 668
BstMBI	GATC	7 cut(s)	493, 598, 615, 684, 754, 760, 804
BstMCI	CGRYCG	2 cut(s)	153, 694
BstMWI	GCNNNNNNNGC	4 cut(s)	152, 371, 563, 572
BstNI	CCWGG	5 cut(s)	362, 457, 470, 499, 526
BstSCI	CCNGG	5 cut(s)	360, 455, 468, 497, 524
BstUI	CGCG	2 cut(s)	575, 585
BstV1I	GCAGC	3 cut(s)	476, 555, 558
BstX2I	RGATCY	1 cut(s)	615
BstYI	RGATCY	1 cut(s)	615
BstZI	CGGCCG	1 cut(s)	150
BsuRI	GGCC	5 cut(s)	152, 359, 473, 914, 968
BtgI	CCRYGG	2 cut(s)	147, 292
BtgZI	GCGATG	2 cut(s)	547, 1026
BtsCI	GGATG	3 cut(s)	20, 412, 802
CfoI	GCGC	2 cut(s)	551, 904
Cfr13I	GGNCC	6 cut(s)	358, 460, 528, 655, 847, 967
CseI	GACGC	3 cut(s)	446, 518, 574
Csp6I	GTAC	3 cut(s)	394, 838, 979
CviAII	CATG	3 cut(s)	355, 733, 938
CviQI	GTAC	3 cut(s)	394, 838, 979
DdeI	CTNAG	1 cut(s)	989
DpnI	GATC	7 cut(s)	495, 600, 617, 686, 756, 762, 806
DpnII	GATC	7 cut(s)	493, 598, 615, 684, 754, 760, 804
DraIII	CACNNNGTG	1 cut(s)	910
EaeI	YGGCCR	2 cut(s)	150, 912
EagI	CGGCCG	1 cut(s)	150
Eam1104I	CTCTTC	4 cut(s)	242, 317, 423, 956
EarI	CTCTTC	4 cut(s)	242, 317, 423, 956
EciI	GGCGGA	2 cut(s)	133, 307
Ecl136II	GAGCTC	1 cut(s)	949
EclXI	CGGCCG	1 cut(s)	150
Eco130I	CCWWGG	1 cut(s)	192
Eco147I	AGGCCT	1 cut(s)	473
Eco24I	GRGCYC	1 cut(s)	951
Eco47I	GGWCC	4 cut(s)	460, 528, 655, 847
Eco52I	CGGCCG	1 cut(s)	150
Eco53kI	GAGCTC	1 cut(s)	949
Eco57I	CTGAAG	2 cut(s)	214, 408
Eco72I	CACGTG	2 cut(s)	896, 907
Eco88I	CYCGRG	1 cut(s)	589
EcoICRI	GAGCTC	1 cut(s)	949
EcoNI	CCTNNNNNAGG	2 cut(s)	191, 229
EcoO109I	RGGNCCY	2 cut(s)	528, 847
EcoRII	CCWGG	5 cut(s)	360, 455, 468, 497, 524
EcoT14I	CCWWGG	1 cut(s)	192
EcoT22I	ATGCAT	1 cut(s)	1018
EcoT38I	GRGCYC	1 cut(s)	951
ErhI	CCWWGG	1 cut(s)	192
Esp3I	CGTCTC	3 cut(s)	115, 592, 668
FaeI	CATG	3 cut(s)	358, 736, 941
FaiI	YATR	5 cut(s)	356, 660, 734, 939, 1018
FaqI	GGGAC	1 cut(s)	860
FatI	CATG	3 cut(s)	354, 732, 937
FauI	CCCGC	6 cut(s)	95, 275, 568, 644, 778, 858
FbaI	TGATCA	1 cut(s)	684
FblI	GTMKAC	2 cut(s)	444, 483
Fnu4HI	GCNGC	7 cut(s)	153, 301, 321, 490, 544, 547, 704
FokI	GGATG	3 cut(s)	7, 399, 809
FriOI	GRGCYC	1 cut(s)	951
Fsp4HI	GCNGC	7 cut(s)	153, 301, 321, 490, 544, 547, 704
FspBI	CTAG	1 cut(s)	960
GlaI	GCGC	2 cut(s)	550, 903
GluI	GCNGC	7 cut(s)	153, 301, 321, 490, 544, 547, 704
GsuI	CTGGAG	1 cut(s)	508
HaeII	RGCGCY	1 cut(s)	552
HaeIII	GGCC	5 cut(s)	152, 359, 473, 914, 968
HapII	CCGG	2 cut(s)	552, 694
HgaI	GACGC	3 cut(s)	446, 518, 574
HhaI	GCGC	2 cut(s)	551, 904
Hin1I	GRCGYC	3 cut(s)	438, 480, 510
Hin1II	CATG	3 cut(s)	358, 736, 941
Hin6I	GCGC	2 cut(s)	549, 902
HinP1I	GCGC	2 cut(s)	549, 902
HincII	GTYRAC	3 cut(s)	445, 484, 517
HindII	GTYRAC	3 cut(s)	445, 484, 517
HinfI	GANTC	8 cut(s)	134, 158, 164, 341, 628, 668, 890, 1004
HpaII	CCGG	2 cut(s)	552, 694
HphI	GGTGA	5 cut(s)	76, 85, 445, 605, 1037
Hpy166II	GTNNAC	5 cut(s)	445, 463, 484, 517, 581
Hpy188I	TCNGA	5 cut(s)	163, 478, 620, 673, 780
Hpy188III	TCNNGA	3 cut(s)	407, 758, 764
Hpy8I	GTNNAC	5 cut(s)	445, 463, 484, 517, 581
Hpy99I	CGWCG	7 cut(s)	392, 446, 482, 485, 488, 784, 1025
HpyAV	CCTTC	5 cut(s)	24, 235, 432, 541, 862
HpyCH4III	ACNGT	3 cut(s)	109, 813, 1025
HpyCH4IV	ACGT	3 cut(s)	480, 895, 906
HpyCH4V	TGCA	1 cut(s)	1016
HpyF10VI	GCNNNNNNNGC	4 cut(s)	152, 371, 563, 572
HpyF3I	CTNAG	1 cut(s)	989
HpySE526I	ACGT	3 cut(s)	480, 895, 906
Hsp92I	GRCGYC	3 cut(s)	438, 480, 510
Hsp92II	CATG	3 cut(s)	358, 736, 941
HspAI	GCGC	2 cut(s)	549, 902
KflI	GGGWCCC	1 cut(s)	847
Ksp22I	TGATCA	1 cut(s)	684
Kzo9I	GATC	7 cut(s)	493, 598, 615, 684, 754, 760, 804
LguI	GCTCTTC	2 cut(s)	242, 956
LmnI	GCTCC	4 cut(s)	83, 409, 554, 946
Lsp1109I	GCAGC	3 cut(s)	476, 555, 558
LweI	GCATC	1 cut(s)	1003
MaeI	CTAG	1 cut(s)	960
MaeII	ACGT	3 cut(s)	480, 895, 906
MaeIII	GTNAC	2 cut(s)	882, 891
MalI	GATC	7 cut(s)	495, 600, 617, 686, 756, 762, 806
MboI	GATC	7 cut(s)	493, 598, 615, 684, 754, 760, 804
MboII	GAAGA	7 cut(s)	9, 220, 229, 304, 410, 625, 943
MflI	RGATCY	1 cut(s)	615
MhlI	GDGCHC	1 cut(s)	951
MlsI	TGGCCA	1 cut(s)	914
MluCI	AATT	1 cut(s)	378
MluI	ACGCGT	1 cut(s)	583
MluNI	TGGCCA	1 cut(s)	914
MlyI	GAGTC	6 cut(s)	143, 158, 167, 335, 899, 1013
MmeI	TCCRAC	3 cut(s)	192, 501, 803
Mox20I	TGGCCA	1 cut(s)	914
Mph1103I	ATGCAT	1 cut(s)	1018
MscI	TGGCCA	1 cut(s)	914
MseI	TTAA	1 cut(s)	206
Msp20I	TGGCCA	1 cut(s)	914
MspA1I	CMGCKG	2 cut(s)	706, 853
MspI	CCGG	2 cut(s)	552, 694
MspR9I	CCNGG	5 cut(s)	362, 457, 470, 499, 526
MvaI	CCWGG	5 cut(s)	362, 457, 470, 499, 526
MvnI	CGCG	2 cut(s)	575, 585
MwoI	GCNNNNNNNGC	4 cut(s)	152, 371, 563, 572
NdeII	GATC	7 cut(s)	493, 598, 615, 684, 754, 760, 804
NlaIII	CATG	3 cut(s)	358, 736, 941
NlaIV	GGNNCC	6 cut(s)	79, 405, 461, 656, 848, 849
NmeAIII	GCCGAG	2 cut(s)	181, 843
NmuCI	GTSAC	1 cut(s)	891
NsiI	ATGCAT	1 cut(s)	1018
PceI	AGGCCT	1 cut(s)	473
PciSI	GCTCTTC	2 cut(s)	242, 956
PcsI	WCGNNNNNNNCGW	3 cut(s)	438, 489, 1008
PfeI	GAWTC	2 cut(s)	628, 668
PflFI	GACNNNGTC	1 cut(s)	440
PflMI	CCANNNNNTGG	1 cut(s)	456
PkrI	GCNGC	7 cut(s)	154, 302, 322, 491, 545, 548, 705
PleI	GAGTC	6 cut(s)	142, 158, 166, 335, 898, 1012
PmaCI	CACGTG	2 cut(s)	896, 907
PmlI	CACGTG	2 cut(s)	896, 907
PpsI	GAGTC	6 cut(s)	142, 158, 166, 335, 898, 1012
Ppu21I	YACGTR	2 cut(s)	896, 907
PpuMI	RGGWCCY	2 cut(s)	528, 847
Psp124BI	GAGCTC	1 cut(s)	951
Psp5II	RGGWCCY	2 cut(s)	528, 847
Psp6I	CCWGG	5 cut(s)	360, 455, 468, 497, 524
PspCI	CACGTG	2 cut(s)	896, 907
PspGI	CCWGG	5 cut(s)	360, 455, 468, 497, 524
PspN4I	GGNNCC	6 cut(s)	79, 405, 461, 656, 848, 849
PspPI	GGNCC	6 cut(s)	358, 460, 528, 655, 847, 967
PspPPI	RGGWCCY	2 cut(s)	528, 847
PsuI	RGATCY	1 cut(s)	615
PsyI	GACNNNGTC	1 cut(s)	440
RsaI	GTAC	3 cut(s)	395, 839, 980
RsaNI	GTAC	3 cut(s)	394, 838, 979
SacI	GAGCTC	1 cut(s)	951
SalI	GTCGAC	2 cut(s)	443, 482
SapI	GCTCTTC	2 cut(s)	242, 956
SaqAI	TTAA	1 cut(s)	206
SatI	GCNGC	7 cut(s)	153, 301, 321, 490, 544, 547, 704
Sau3AI	GATC	7 cut(s)	493, 598, 615, 684, 754, 760, 804
Sau96I	GGNCC	6 cut(s)	358, 460, 528, 655, 847, 967
SchI	GAGTC	6 cut(s)	143, 158, 167, 335, 899, 1013
ScrFI	CCNGG	5 cut(s)	362, 457, 470, 499, 526
SduI	GDGCHC	1 cut(s)	951
SfaNI	GCATC	1 cut(s)	1003
SgrDI	CGTCGACG	1 cut(s)	482
SinI	GGWCC	4 cut(s)	460, 528, 655, 847
SmlI	CTYRAG	1 cut(s)	887
SmoI	CTYRAG	1 cut(s)	887
Sse9I	AATT	1 cut(s)	378
SseBI	AGGCCT	1 cut(s)	473
SspI	AATATT	1 cut(s)	1033
SspMI	CTAG	1 cut(s)	960
SstI	GAGCTC	1 cut(s)	951
StuI	AGGCCT	1 cut(s)	473
StyD4I	CCNGG	5 cut(s)	360, 455, 468, 497, 524
StyI	CCWWGG	1 cut(s)	192
TaaI	ACNGT	3 cut(s)	109, 813, 1025
TaiI	ACGT	3 cut(s)	483, 898, 909
TaqI	TCGA	6 cut(s)	390, 444, 483, 807, 954, 1002
TasI	AATT	1 cut(s)	378
TauI	GCSGC	4 cut(s)	155, 303, 323, 706
TfiI	GAWTC	2 cut(s)	628, 668
Tru1I	TTAA	1 cut(s)	206
Tru9I	TTAA	1 cut(s)	206
TseFI	GTSAC	1 cut(s)	891
TseI	GCWGC	3 cut(s)	489, 543, 546
Tsp45I	GTSAC	1 cut(s)	891
TspDTI	ATGAA	3 cut(s)	9, 626, 926
TspGWI	ACGGA	2 cut(s)	309, 376
Tth111I	GACNNNGTC	1 cut(s)	440
Van91I	CCANNNNNTGG	1 cut(s)	456
VpaK11BI	GGWCC	4 cut(s)	460, 528, 655, 847
XagI	CCTNNNNNAGG	2 cut(s)	191, 229
XmiI	GTMKAC	2 cut(s)	444, 483
XspI	CTAG	1 cut(s)	960
ZraI	GACGTC	1 cut(s)	481
Zsp2I	ATGCAT	1 cut(s)	1018

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.