RLG00000028410

carboxylesterase 8

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0005485
Physical Location & Seq
Reverse (-)
22497915 .. 22498940
1026 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000028410

Sequence Viewer

Length: 1026 bp
ATGGCAGATCAACAATCCTCAATCGACCCCTACAAGTTCCTCAAAATCGTTCCCAACCCAGATGGCTCACTCACCCGCCTCGCCCCAATCCCAATCGCACCCACAACCGAGTCAATCCCCGCCGATTCTGATCCCAACTCATCCCAACTCGTCCTCTCCAAGGACATCCCTCTCGACCCAAAATCCCAAACCTCCCTTCGCCTCTTCAAACCCCTCAATCTCCCCCCAAACCCAAACCATAACCTCCCTCTCCTCATCTACTACCACGGCGGCGGCTTCATCATCGGCAGCGCCGCCTCTCTGCCTTTCCACAACTGCTGCTCTCGCCTCGCCCTCTCTATCCCTGCCCTAGTCCTCTCCGTCGAGTACCGTCTAGCCCCCGAGCACCGCCTCCCCGCCGCCTTCGACGACGCCGTTTCGGCCATCGCCTGGCTCAAAAACCAGGCCTCCTCTGCCGACGGAGAATCGTGGCTGAAAAACTCCGTTGATTTCTCCAAGTGTTTCATCATGGGAACTAGCGCGGGATCCAGTATCGCCTACCACGCGGGTCTACGGGCTTCCGATATGGACCTCTCCCCAATCAAAATCCGAGGGCTGATATTGCACCAGGCGTATTTCGGGGGGGTCAACAGGACCGAATCCGAGATCAGGTTAGTCAACGACCGGGTGCTGCCGTTAGTTGCTACCGACTTGATGTGGGCCCTGAGCCTGCCGGAGGGGGCTGATCGTGACCACGAGTATTGTAATCCATCTATCAACGGCGGTGATGGAAGGATCGGACGGTTGCCGTGGTGTTTGGTTAGCGGGTACGGCGGCGACCCGCTGGTGGATAGGCAGAAGGAGATGGCGGCATTGTTGGAGTCACGCGGGGTGCACGTGACGGCCCGGTTTGAGGAGGGTGGTTTTCATGGTGCGGAATTTGACCCCAACAAGGCCGACGCATTCTATGAGAATGTTGAGGAATTTATCGGCTCTTGTTGTGCATACGGCGGCGATGATGTTGTCGTTGCAAAATCGACCATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0009056 GO:0016787
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

342

Amino Acids

36.86

Weight (kDa)

5.17

Isoelectric Point (pI)

36.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BD-FAE PF20434 73 - 189 3.3e-13 BD-FAE
Abhydrolase_3 PF07859 85 - 305 3.6e-53 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 429
AccI GTMKAC 1 cut(s) 550
AccII CGCG 3 cut(s) 521, 545, 867
AclWI GGATC 4 cut(s) 125, 519, 532, 782
AcoI YGGCCR 1 cut(s) 420
AcsI RAATTY 2 cut(s) 917, 962
AcvI CACGTG 1 cut(s) 877
AcyI GRCGYC 1 cut(s) 411
AfaI GTAC 2 cut(s) 368, 809
AfiI CCNNNNNNNGG 7 cut(s) 160, 429, 648, 715, 826, 892, 931
AgsI TTSAA 1 cut(s) 208
AjnI CCWGG 3 cut(s) 428, 441, 606
AjuI GAANNNNNNNTTGG 2 cut(s) 180, 212
Alw21I GWGCWC 2 cut(s) 387, 876
Alw44I GTGCAC 1 cut(s) 872
AlwI GGATC 4 cut(s) 125, 519, 532, 782
Ama87I CYCGRG 1 cut(s) 380
AoxI GGCC 5 cut(s) 420, 444, 699, 882, 933
ApaI GGGCCC 1 cut(s) 703
ApaLI GTGCAC 1 cut(s) 872
ApeKI GCWGC 3 cut(s) 288, 318, 670
ApoI RAATTY 2 cut(s) 917, 962
AspLEI GCGC 2 cut(s) 293, 521
AspS9I GGNCC 5 cut(s) 568, 633, 699, 700, 883
AsuC2I CCSGG 2 cut(s) 665, 886
AsuHPI GGTGA 2 cut(s) 64, 776
AvaI CYCGRG 1 cut(s) 380
AvaII GGWCC 2 cut(s) 568, 633
BaeGI GKGCMC 2 cut(s) 703, 876
BamHI GGATCC 1 cut(s) 524
BanII GRGCYC 1 cut(s) 703
BauI CACGAG 1 cut(s) 734
BbrPI CACGTG 1 cut(s) 877
Bbv12I GWGCWC 2 cut(s) 387, 876
BbvI GCAGC 3 cut(s) 300, 305, 657
BccI CCATC 5 cut(s) 56, 431, 757, 761, 838
BceAI ACGGC 8 cut(s) 283, 398, 658, 772, 775, 826, 897, 1003
BciT130I CCWGG 3 cut(s) 430, 443, 608
BcnI CCSGG 2 cut(s) 665, 886
BfaI CTAG 3 cut(s) 350, 374, 516
BfoI RGCGCY 1 cut(s) 294
BglI GCCNNNNNGGC 1 cut(s) 419
Bme1390I CCNGG 5 cut(s) 430, 443, 608, 665, 886
Bme18I GGWCC 2 cut(s) 568, 633
BmeT110I CYCGRG 1 cut(s) 380
BmgT120I GGNCC 5 cut(s) 568, 633, 699, 700, 883
BmiI GGNNCC 2 cut(s) 526, 701
BmrFI CCNGG 5 cut(s) 430, 443, 608, 665, 886
Bpu10I CCTNAGC 1 cut(s) 704
BpuMI CCSGG 2 cut(s) 665, 886
BsaAI YACGTR 1 cut(s) 877
BsaBI GATNNNNATC 1 cut(s) 129
BsaHI GRCGYC 1 cut(s) 411
BsaJI CCNNGG 4 cut(s) 159, 265, 589, 788
BsaXI ACNNNNNCTCC 2 cut(s) 431, 461
Bsc4I CCNNNNNNNGG 7 cut(s) 160, 429, 648, 715, 826, 892, 931
Bse1I ACTGG 1 cut(s) 528
Bse8I GATNNNNATC 1 cut(s) 129
BseBI CCWGG 3 cut(s) 430, 443, 608
BseDI CCNNGG 4 cut(s) 159, 265, 589, 788
BseGI GGATG 2 cut(s) 140, 165
BseJI GATNNNNATC 1 cut(s) 129
BseLI CCNNNNNNNGG 7 cut(s) 160, 429, 648, 715, 826, 892, 931
BseMII CTCAG 1 cut(s) 695
BseNI ACTGG 1 cut(s) 528
BseRI GAGGAG 3 cut(s) 242, 439, 908
BseSI GKGCMC 2 cut(s) 703, 876
BseXI GCAGC 3 cut(s) 300, 305, 657
Bsh1236I CGCG 3 cut(s) 521, 545, 867
Bsh1285I CGRYCG 1 cut(s) 664
BshFI GGCC 5 cut(s) 422, 446, 701, 884, 935
BsiEI CGRYCG 1 cut(s) 664
BsiHKAI GWGCWC 2 cut(s) 387, 876
BsiHKCI CYCGRG 1 cut(s) 380
BsiSI CCGG 3 cut(s) 664, 713, 886
BslI CCNNNNNNNGG 7 cut(s) 160, 429, 648, 715, 826, 892, 931
BsmI GAATGC 1 cut(s) 941
BsnI GGCC 5 cut(s) 422, 446, 701, 884, 935
BsoBI CYCGRG 1 cut(s) 380
Bsp120I GGGCCC 1 cut(s) 699
Bsp1286I GDGCHC 3 cut(s) 387, 703, 876
Bsp143I GATC 6 cut(s) 7, 130, 524, 645, 724, 774
BspANI GGCC 5 cut(s) 422, 446, 701, 884, 935
BspCNI CTCAG 1 cut(s) 696
BspFNI CGCG 3 cut(s) 521, 545, 867
BspLI GGNNCC 2 cut(s) 526, 701
BspPI GGATC 4 cut(s) 125, 519, 532, 782
BsrI ACTGG 1 cut(s) 528
BssECI CCNNGG 4 cut(s) 159, 265, 589, 788
BssMI GATC 6 cut(s) 7, 130, 524, 645, 724, 774
BssNI GRCGYC 1 cut(s) 411
BssSI CACGAG 1 cut(s) 734
BssT1I CCWWGG 1 cut(s) 159
Bst2BI CACGAG 1 cut(s) 734
Bst2UI CCWGG 3 cut(s) 430, 443, 608
Bst4CI ACNGT 2 cut(s) 371, 783
Bst6I CTCTTC 1 cut(s) 209
BstACI GRCGYC 1 cut(s) 411
BstBAI YACGTR 1 cut(s) 877
BstC8I GCNNGC 1 cut(s) 710
BstDEI CTNAG 1 cut(s) 704
BstDSI CCRYGG 2 cut(s) 265, 788
BstENI CCTNNNNNAGG 2 cut(s) 158, 713
BstF5I GGATG 2 cut(s) 140, 165
BstFNI CGCG 3 cut(s) 521, 545, 867
BstH2I RGCGCY 1 cut(s) 294
BstHHI GCGC 2 cut(s) 293, 521
BstKTI GATC 6 cut(s) 10, 133, 527, 648, 727, 777
BstMBI GATC 6 cut(s) 7, 130, 524, 645, 724, 774
BstMCI CGRYCG 1 cut(s) 664
BstMWI GCNNNNNNNGC 6 cut(s) 324, 419, 452, 542, 601, 810
BstNI CCWGG 3 cut(s) 430, 443, 608
BstSCI CCNGG 5 cut(s) 428, 441, 606, 663, 884
BstSLI GKGCMC 2 cut(s) 703, 876
BstUI CGCG 3 cut(s) 521, 545, 867
BstV1I GCAGC 3 cut(s) 300, 305, 657
BstX2I RGATCY 1 cut(s) 524
BstYI RGATCY 1 cut(s) 524
BsuRI GGCC 5 cut(s) 422, 446, 701, 884, 935
BtgI CCRYGG 2 cut(s) 265, 788
BtgZI GCGATG 2 cut(s) 409, 1008
BtsCI GGATG 2 cut(s) 140, 165
Cac8I GCNNGC 1 cut(s) 710
CfoI GCGC 2 cut(s) 293, 521
Cfr13I GGNCC 5 cut(s) 568, 633, 699, 700, 883
CseI GACGC 2 cut(s) 419, 947
Csp6I GTAC 2 cut(s) 367, 808
CspCI CAANNNNNGTGG 2 cut(s) 722, 757
CviAII CATG 2 cut(s) 508, 908
CviQI GTAC 2 cut(s) 367, 808
DdeI CTNAG 1 cut(s) 704
DpnI GATC 6 cut(s) 9, 132, 526, 647, 726, 776
DpnII GATC 6 cut(s) 7, 130, 524, 645, 724, 774
EaeI YGGCCR 1 cut(s) 420
Eam1104I CTCTTC 1 cut(s) 209
EarI CTCTTC 1 cut(s) 209
Eco130I CCWWGG 1 cut(s) 159
Eco147I AGGCCT 1 cut(s) 446
Eco24I GRGCYC 1 cut(s) 703
Eco47I GGWCC 2 cut(s) 568, 633
Eco72I CACGTG 1 cut(s) 877
Eco88I CYCGRG 1 cut(s) 380
EcoNI CCTNNNNNAGG 2 cut(s) 158, 713
EcoO109I RGGNCCY 1 cut(s) 700
EcoRII CCWGG 3 cut(s) 428, 441, 606
EcoT14I CCWWGG 1 cut(s) 159
EcoT38I GRGCYC 1 cut(s) 703
ErhI CCWWGG 1 cut(s) 159
FaeI CATG 2 cut(s) 511, 911
FaiI YATR 8 cut(s) 240, 509, 566, 909, 948, 985, 1022, 1024
FatI CATG 2 cut(s) 507, 907
FauI CCCGC 8 cut(s) 83, 127, 403, 514, 538, 797, 828, 860
FauNDI CATATG 1 cut(s) 1022
FblI GTMKAC 1 cut(s) 550
FokI GGATG 2 cut(s) 127, 152
FriOI GRGCYC 1 cut(s) 703
FspBI CTAG 3 cut(s) 350, 374, 516
GlaI GCGC 2 cut(s) 292, 520
HaeII RGCGCY 1 cut(s) 294
HaeIII GGCC 5 cut(s) 422, 446, 701, 884, 935
HapII CCGG 3 cut(s) 664, 713, 886
HgaI GACGC 2 cut(s) 419, 947
HhaI GCGC 2 cut(s) 293, 521
Hin1I GRCGYC 1 cut(s) 411
Hin1II CATG 2 cut(s) 511, 911
Hin6I GCGC 2 cut(s) 291, 519
HinP1I GCGC 2 cut(s) 291, 519
HincII GTYRAC 2 cut(s) 628, 658
HindII GTYRAC 2 cut(s) 628, 658
HinfI GANTC 5 cut(s) 110, 125, 464, 638, 860
HpaII CCGG 3 cut(s) 664, 713, 886
HphI GGTGA 2 cut(s) 64, 776
Hpy166II GTNNAC 4 cut(s) 551, 628, 658, 874
Hpy188I TCNGA 5 cut(s) 130, 562, 590, 643, 779
Hpy188III TCNNGA 2 cut(s) 173, 728
Hpy8I GTNNAC 4 cut(s) 551, 628, 658, 874
Hpy99I CGWCG 5 cut(s) 365, 410, 413, 461, 941
HpyAV CCTTC 4 cut(s) 206, 412, 765, 832
HpyCH4III ACNGT 2 cut(s) 371, 783
HpyCH4IV ACGT 1 cut(s) 876
HpyCH4V TGCA 4 cut(s) 604, 874, 983, 1010
HpyF10VI GCNNNNNNNGC 6 cut(s) 324, 419, 452, 542, 601, 810
HpyF3I CTNAG 1 cut(s) 704
HpySE526I ACGT 1 cut(s) 876
Hsp92I GRCGYC 1 cut(s) 411
Hsp92II CATG 2 cut(s) 511, 911
HspAI GCGC 2 cut(s) 291, 519
Kzo9I GATC 6 cut(s) 7, 130, 524, 645, 724, 774
Lsp1109I GCAGC 3 cut(s) 300, 305, 657
MaeI CTAG 3 cut(s) 350, 374, 516
MaeII ACGT 1 cut(s) 876
MaeIII GTNAC 3 cut(s) 728, 861, 877
MalI GATC 6 cut(s) 9, 132, 526, 647, 726, 776
MboI GATC 6 cut(s) 7, 130, 524, 645, 724, 774
MboII GAAGA 1 cut(s) 196
MflI RGATCY 1 cut(s) 524
MhlI GDGCHC 3 cut(s) 387, 703, 876
MluCI AATT 2 cut(s) 917, 962
MlyI GAGTC 2 cut(s) 119, 869
MmeI TCCRAC 1 cut(s) 837
MspA1I CMGCKG 1 cut(s) 823
MspI CCGG 3 cut(s) 664, 713, 886
MspR9I CCNGG 5 cut(s) 430, 443, 608, 665, 886
Mva1269I GAATGC 1 cut(s) 941
MvaI CCWGG 3 cut(s) 430, 443, 608
MvnI CGCG 3 cut(s) 521, 545, 867
MwoI GCNNNNNNNGC 6 cut(s) 324, 419, 452, 542, 601, 810
NciI CCSGG 2 cut(s) 665, 886
NdeI CATATG 1 cut(s) 1022
NdeII GATC 6 cut(s) 7, 130, 524, 645, 724, 774
NlaIII CATG 2 cut(s) 511, 911
NlaIV GGNNCC 2 cut(s) 526, 701
NmuCI GTSAC 3 cut(s) 728, 861, 877
PceI AGGCCT 1 cut(s) 446
PcsI WCGNNNNNNNCGW 1 cut(s) 411
PctI GAATGC 1 cut(s) 941
PfeI GAWTC 3 cut(s) 125, 464, 638
PflMI CCANNNNNTGG 1 cut(s) 429
PleI GAGTC 2 cut(s) 118, 868
PmaCI CACGTG 1 cut(s) 877
PmlI CACGTG 1 cut(s) 877
PpsI GAGTC 2 cut(s) 118, 868
Ppu21I YACGTR 1 cut(s) 877
Psp6I CCWGG 3 cut(s) 428, 441, 606
PspCI CACGTG 1 cut(s) 877
PspGI CCWGG 3 cut(s) 428, 441, 606
PspN4I GGNNCC 2 cut(s) 526, 701
PspOMI GGGCCC 1 cut(s) 699
PspPI GGNCC 5 cut(s) 568, 633, 699, 700, 883
PsuI RGATCY 1 cut(s) 524
RsaI GTAC 2 cut(s) 368, 809
RsaNI GTAC 2 cut(s) 367, 808
Sau3AI GATC 6 cut(s) 7, 130, 524, 645, 724, 774
Sau96I GGNCC 5 cut(s) 568, 633, 699, 700, 883
SchI GAGTC 2 cut(s) 119, 869
ScrFI CCNGG 5 cut(s) 430, 443, 608, 665, 886
SduI GDGCHC 3 cut(s) 387, 703, 876
SetI ASST 5 cut(s) 194, 246, 573, 653, 879
SinI GGWCC 2 cut(s) 568, 633
Sse9I AATT 2 cut(s) 917, 962
SseBI AGGCCT 1 cut(s) 446
SspMI CTAG 3 cut(s) 350, 374, 516
StuI AGGCCT 1 cut(s) 446
StyD4I CCNGG 5 cut(s) 428, 441, 606, 663, 884
StyI CCWWGG 1 cut(s) 159
TaaI ACNGT 2 cut(s) 371, 783
TaiI ACGT 1 cut(s) 879
TaqI TCGA 5 cut(s) 24, 174, 363, 405, 1016
TaqII GACCGA 1 cut(s) 650
TasI AATT 2 cut(s) 917, 962
TauI GCSGC 7 cut(s) 273, 276, 296, 401, 816, 851, 993
TfiI GAWTC 3 cut(s) 125, 464, 638
TseFI GTSAC 3 cut(s) 728, 861, 877
TseI GCWGC 3 cut(s) 288, 318, 670
Tsp45I GTSAC 3 cut(s) 728, 861, 877
TspDTI ATGAA 3 cut(s) 268, 493, 896
TspGWI ACGGA 3 cut(s) 349, 472, 474
Van91I CCANNNNNTGG 1 cut(s) 429
VneI GTGCAC 1 cut(s) 872
VpaK11BI GGWCC 2 cut(s) 568, 633
XagI CCTNNNNNAGG 2 cut(s) 158, 713
XapI RAATTY 2 cut(s) 917, 962
XmiI GTMKAC 1 cut(s) 550
XspI CTAG 3 cut(s) 350, 374, 516
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.