Rh1AG232400

carboxylesterase 8

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0005485
Physical Location & Seq
Forward (+)
44205675 .. 44207245
1571 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG232400.1

Sequence Viewer

Length: 1029 bp
ATGGCAGATCAACAATCCTCAATCGACCCCTACAAGTTCCTCAAAATCGTTCCCAACCCAGATGGCTCACTCACCCGCCTCGCCCCACTCACAATCGCACCCACAACCGAGTCAATCCCCGCCGATTCTGATCCCAACTCATCCCAACTCGTCCTCTCCAAGGACATCCCTCTCGACCCAAAATCCCAAACCTCCCTTCGCCTCTTCAAACCCCTCAATCTCCCCCCAAACCCAAACCCTAACCTCCCTCTCCTCATCTACATCCACGGCGGCGGCTTCATCATCTGGAGCGCCGCCTCTCAGCCTTTCCACCACTACTGCTCTCGCCTCGCCCTCTCCATCCCAGCCCTAGTCCTCTCCGTCGAGTACCGTCTCGCCCCCGAGCACCGCCTCCCCGCCGCCTACGACGACGTCGTTTCGGCCATCACCTGGCTCAAAAACCAGGCCTCCTCTGCCGACAGAGACTCGTGGCTGAAAAACTCCGTTGATTTCTCCAAGTGTTTCATCATGGGAACTAGCGCGGGATCCAATATCGCCTACCACGCGGGTCTACGCGCTTCCGATATGGACCTCTCCCCGATCAAAATCCGAGGGCTGATATTGCACCAGGCGTATTTCGGCGGGGTCAACAGGACCGAATCCGAGATCAGGTTAGTCAACGACCGGGTGCTGCCGTTAGTTGCTAACGACTTGATGTGGGCCCTGAGCCTGCCGGAGGGGGCTGATCGTGACCACGAGTATTGTAATCCATCTATCAACGGCGGTGATGGAAGGATCGGACGGTTGCCGTGGTGTTTGGTGAGCGGGAATGGCGGGGACCCGCTGGTGGATAGGCAGAAGGAGATCGCGGCACTGTTGGAGTCACGCGGGGTGCACGTGACGGCCCGGTTTGAGGAGGGTGGTTTTCATGGTGCGGAGCTATTTGACCCCAACAAGGCCGAGGCTTTCTATGAGATTGTTAAGGAATTTATCGGCTCTTGTTGCGCACGCGGTAGCGATGATGTTGCCGTGGTAAAATCAGCCATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0009056 GO:0016787
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

342

Amino Acids

37.27

Weight (kDa)

5.6

Isoelectric Point (pI)

36.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
BD-FAE PF20434 73 - 189 6.3e-15 BD-FAE
Abhydrolase_3 PF07859 85 - 305 1.5e-52 alpha/beta hydrolase fold
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 414
Acc16I TGCGCA 1 cut(s) 985
AccB7I CCANNNNNTGG 1 cut(s) 429
AccBSI CCGCTC 1 cut(s) 804
AccI GTMKAC 1 cut(s) 550
AccII CGCG 6 cut(s) 521, 545, 555, 848, 867, 990
AclWI GGATC 4 cut(s) 125, 519, 532, 782
AcoI YGGCCR 1 cut(s) 420
AcsI RAATTY 1 cut(s) 965
AcvI CACGTG 1 cut(s) 877
AcyI GRCGYC 1 cut(s) 411
AfaI GTAC 1 cut(s) 368
AfiI CCNNNNNNNGG 7 cut(s) 160, 429, 648, 715, 826, 892, 934
AgsI TTSAA 1 cut(s) 208
AjnI CCWGG 3 cut(s) 428, 441, 606
AjuI GAANNNNNNNTTGG 2 cut(s) 180, 212
AluBI AGCT 1 cut(s) 919
AluI AGCT 1 cut(s) 919
Alw21I GWGCWC 2 cut(s) 387, 876
Alw26I GTCTC 2 cut(s) 377, 456
Alw44I GTGCAC 1 cut(s) 872
AlwI GGATC 4 cut(s) 125, 519, 532, 782
Ama87I CYCGRG 1 cut(s) 380
AoxI GGCC 5 cut(s) 420, 444, 699, 882, 936
ApaI GGGCCC 1 cut(s) 703
ApaLI GTGCAC 1 cut(s) 872
ApeKI GCWGC 1 cut(s) 670
ApoI RAATTY 1 cut(s) 965
AspLEI GCGC 4 cut(s) 293, 521, 557, 986
AspS9I GGNCC 6 cut(s) 568, 633, 699, 700, 817, 883
AsuC2I CCSGG 2 cut(s) 665, 886
AsuHPI GGTGA 4 cut(s) 64, 418, 776, 811
AvaI CYCGRG 1 cut(s) 380
AvaII GGWCC 3 cut(s) 568, 633, 817
BaeGI GKGCMC 2 cut(s) 703, 876
BamHI GGATCC 1 cut(s) 524
BanII GRGCYC 1 cut(s) 703
BauI CACGAG 2 cut(s) 466, 734
BbrPI CACGTG 1 cut(s) 877
Bbv12I GWGCWC 2 cut(s) 387, 876
BbvI GCAGC 1 cut(s) 657
BccI CCATC 5 cut(s) 56, 347, 431, 757, 761
BceAI ACGGC 6 cut(s) 283, 658, 772, 775, 897, 992
BcgI CGANNNNNNTGC 2 cut(s) 986, 1020
BciT130I CCWGG 3 cut(s) 430, 443, 608
BcnI CCSGG 2 cut(s) 665, 886
BcoDI GTCTC 2 cut(s) 377, 456
BfaI CTAG 2 cut(s) 350, 516
BfoI RGCGCY 1 cut(s) 294
BisI GCNGC 6 cut(s) 271, 274, 294, 399, 671, 849
BlsI GCNGC 6 cut(s) 272, 275, 295, 400, 672, 850
Bme1390I CCNGG 5 cut(s) 430, 443, 608, 665, 886
Bme18I GGWCC 3 cut(s) 568, 633, 817
BmeT110I CYCGRG 1 cut(s) 380
BmgT120I GGNCC 6 cut(s) 568, 633, 699, 700, 817, 883
BmiI GGNNCC 4 cut(s) 526, 701, 818, 819
BmrFI CCNGG 5 cut(s) 430, 443, 608, 665, 886
BpmI CTGGAG 1 cut(s) 307
Bpu10I CCTNAGC 1 cut(s) 704
BpuMI CCSGG 2 cut(s) 665, 886
BsaAI YACGTR 1 cut(s) 877
BsaBI GATNNNNATC 2 cut(s) 129, 584
BsaHI GRCGYC 1 cut(s) 411
BsaJI CCNNGG 6 cut(s) 159, 265, 589, 788, 939, 1008
BsaXI ACNNNNNCTCC 2 cut(s) 431, 461
Bsc4I CCNNNNNNNGG 7 cut(s) 160, 429, 648, 715, 826, 892, 934
Bse8I GATNNNNATC 2 cut(s) 129, 584
BseBI CCWGG 3 cut(s) 430, 443, 608
BseDI CCNNGG 6 cut(s) 159, 265, 589, 788, 939, 1008
BseGI GGATG 4 cut(s) 140, 165, 261, 339
BseJI GATNNNNATC 2 cut(s) 129, 584
BseLI CCNNNNNNNGG 7 cut(s) 160, 429, 648, 715, 826, 892, 934
BseMII CTCAG 2 cut(s) 314, 695
BseRI GAGGAG 3 cut(s) 242, 439, 908
BseSI GKGCMC 2 cut(s) 703, 876
BseXI GCAGC 1 cut(s) 657
BseYI CCCAGC 1 cut(s) 343
Bsh1236I CGCG 6 cut(s) 521, 545, 555, 848, 867, 990
Bsh1285I CGRYCG 1 cut(s) 664
BshFI GGCC 5 cut(s) 422, 446, 701, 884, 938
BsiEI CGRYCG 1 cut(s) 664
BsiHKAI GWGCWC 2 cut(s) 387, 876
BsiHKCI CYCGRG 1 cut(s) 380
BsiSI CCGG 3 cut(s) 664, 713, 886
BslFI GGGAC 1 cut(s) 830
BslI CCNNNNNNNGG 7 cut(s) 160, 429, 648, 715, 826, 892, 934
BsmAI GTCTC 2 cut(s) 377, 456
BsmBI CGTCTC 1 cut(s) 377
BsmFI GGGAC 1 cut(s) 830
BsnI GGCC 5 cut(s) 422, 446, 701, 884, 938
BsoBI CYCGRG 1 cut(s) 380
Bsp120I GGGCCC 1 cut(s) 699
Bsp1286I GDGCHC 3 cut(s) 387, 703, 876
Bsp143I GATC 8 cut(s) 7, 130, 524, 579, 645, 724, 774, 843
BspANI GGCC 5 cut(s) 422, 446, 701, 884, 938
BspCNI CTCAG 2 cut(s) 313, 696
BspFNI CGCG 6 cut(s) 521, 545, 555, 848, 867, 990
BspLI GGNNCC 4 cut(s) 526, 701, 818, 819
BspPI GGATC 4 cut(s) 125, 519, 532, 782
BsrBI CCGCTC 1 cut(s) 804
BssECI CCNNGG 6 cut(s) 159, 265, 589, 788, 939, 1008
BssMI GATC 8 cut(s) 7, 130, 524, 579, 645, 724, 774, 843
BssNI GRCGYC 1 cut(s) 411
BssSI CACGAG 2 cut(s) 466, 734
BssT1I CCWWGG 1 cut(s) 159
Bst2BI CACGAG 2 cut(s) 466, 734
Bst2UI CCWGG 3 cut(s) 430, 443, 608
Bst4CI ACNGT 3 cut(s) 371, 783, 855
Bst6I CTCTTC 1 cut(s) 209
BstACI GRCGYC 1 cut(s) 411
BstBAI YACGTR 1 cut(s) 877
BstC8I GCNNGC 2 cut(s) 710, 988
BstDEI CTNAG 2 cut(s) 300, 704
BstDSI CCRYGG 3 cut(s) 265, 788, 1008
BstENI CCTNNNNNAGG 2 cut(s) 158, 713
BstF5I GGATG 4 cut(s) 140, 165, 261, 339
BstFNI CGCG 6 cut(s) 521, 545, 555, 848, 867, 990
BstH2I RGCGCY 1 cut(s) 294
BstHHI GCGC 4 cut(s) 293, 521, 557, 986
BstKTI GATC 8 cut(s) 10, 133, 527, 582, 648, 727, 777, 846
BstMAI GTCTC 2 cut(s) 377, 456
BstMBI GATC 8 cut(s) 7, 130, 524, 579, 645, 724, 774, 843
BstMCI CGRYCG 1 cut(s) 664
BstMWI GCNNNNNNNGC 5 cut(s) 452, 542, 601, 810, 981
BstNI CCWGG 3 cut(s) 430, 443, 608
BstSCI CCNGG 5 cut(s) 428, 441, 606, 663, 884
BstSLI GKGCMC 2 cut(s) 703, 876
BstUI CGCG 6 cut(s) 521, 545, 555, 848, 867, 990
BstV1I GCAGC 1 cut(s) 657
BstX2I RGATCY 1 cut(s) 524
BstYI RGATCY 1 cut(s) 524
BsuRI GGCC 5 cut(s) 422, 446, 701, 884, 938
BtgI CCRYGG 3 cut(s) 265, 788, 1008
BtgZI GCGATG 1 cut(s) 1011
BtsCI GGATG 4 cut(s) 140, 165, 261, 339
BtsIMutI CAGTG 1 cut(s) 851
Cac8I GCNNGC 2 cut(s) 710, 988
CfoI GCGC 4 cut(s) 293, 521, 557, 986
Cfr13I GGNCC 6 cut(s) 568, 633, 699, 700, 817, 883
Csp6I GTAC 1 cut(s) 367
CspCI CAANNNNNGTGG 2 cut(s) 722, 757
CviAII CATG 3 cut(s) 508, 908, 1024
CviQI GTAC 1 cut(s) 367
DdeI CTNAG 2 cut(s) 300, 704
DpnI GATC 8 cut(s) 9, 132, 526, 581, 647, 726, 776, 845
DpnII GATC 8 cut(s) 7, 130, 524, 579, 645, 724, 774, 843
EaeI YGGCCR 1 cut(s) 420
Eam1104I CTCTTC 1 cut(s) 209
EarI CTCTTC 1 cut(s) 209
Eco130I CCWWGG 1 cut(s) 159
Eco147I AGGCCT 1 cut(s) 446
Eco24I GRGCYC 1 cut(s) 703
Eco47I GGWCC 3 cut(s) 568, 633, 817
Eco72I CACGTG 1 cut(s) 877
Eco88I CYCGRG 1 cut(s) 380
EcoNI CCTNNNNNAGG 2 cut(s) 158, 713
EcoO109I RGGNCCY 2 cut(s) 700, 817
EcoRII CCWGG 3 cut(s) 428, 441, 606
EcoT14I CCWWGG 1 cut(s) 159
EcoT38I GRGCYC 1 cut(s) 703
ErhI CCWWGG 1 cut(s) 159
Esp3I CGTCTC 1 cut(s) 377
FaeI CATG 3 cut(s) 511, 911, 1027
FaiI YATR 5 cut(s) 509, 566, 909, 951, 1025
FaqI GGGAC 1 cut(s) 830
FatI CATG 3 cut(s) 507, 907, 1023
FblI GTMKAC 1 cut(s) 550
Fnu4HI GCNGC 6 cut(s) 271, 274, 294, 399, 671, 849
FokI GGATG 4 cut(s) 127, 152, 248, 326
FriOI GRGCYC 1 cut(s) 703
Fsp4HI GCNGC 6 cut(s) 271, 274, 294, 399, 671, 849
FspBI CTAG 2 cut(s) 350, 516
FspI TGCGCA 1 cut(s) 985
GlaI GCGC 4 cut(s) 292, 520, 556, 985
GluI GCNGC 6 cut(s) 271, 274, 294, 399, 671, 849
GsaI CCCAGC 1 cut(s) 347
GsuI CTGGAG 1 cut(s) 307
HaeII RGCGCY 1 cut(s) 294
HaeIII GGCC 5 cut(s) 422, 446, 701, 884, 938
HapII CCGG 3 cut(s) 664, 713, 886
HhaI GCGC 4 cut(s) 293, 521, 557, 986
Hin1I GRCGYC 1 cut(s) 411
Hin1II CATG 3 cut(s) 511, 911, 1027
Hin6I GCGC 4 cut(s) 291, 519, 555, 984
HinP1I GCGC 4 cut(s) 291, 519, 555, 984
HincII GTYRAC 2 cut(s) 628, 658
HindII GTYRAC 2 cut(s) 628, 658
HinfI GANTC 5 cut(s) 110, 125, 464, 638, 860
HpaII CCGG 3 cut(s) 664, 713, 886
HphI GGTGA 4 cut(s) 64, 418, 776, 811
Hpy166II GTNNAC 4 cut(s) 551, 628, 658, 874
Hpy188I TCNGA 5 cut(s) 130, 562, 590, 643, 779
Hpy188III TCNNGA 3 cut(s) 173, 286, 728
Hpy8I GTNNAC 4 cut(s) 551, 628, 658, 874
Hpy99I CGWCG 4 cut(s) 365, 410, 413, 416
HpyAV CCTTC 3 cut(s) 206, 765, 832
HpyCH4III ACNGT 3 cut(s) 371, 783, 855
HpyCH4IV ACGT 2 cut(s) 411, 876
HpyCH4V TGCA 2 cut(s) 604, 874
HpyF10VI GCNNNNNNNGC 5 cut(s) 452, 542, 601, 810, 981
HpyF3I CTNAG 2 cut(s) 300, 704
HpySE526I ACGT 2 cut(s) 411, 876
Hsp92I GRCGYC 1 cut(s) 411
Hsp92II CATG 3 cut(s) 511, 911, 1027
HspAI GCGC 4 cut(s) 291, 519, 555, 984
KflI GGGWCCC 1 cut(s) 817
Kzo9I GATC 8 cut(s) 7, 130, 524, 579, 645, 724, 774, 843
LmnI GCTCC 2 cut(s) 288, 916
Lsp1109I GCAGC 1 cut(s) 657
MaeI CTAG 2 cut(s) 350, 516
MaeII ACGT 2 cut(s) 411, 876
MaeIII GTNAC 3 cut(s) 728, 861, 877
MalI GATC 8 cut(s) 9, 132, 526, 581, 647, 726, 776, 845
MbiI CCGCTC 1 cut(s) 804
MboI GATC 8 cut(s) 7, 130, 524, 579, 645, 724, 774, 843
MboII GAAGA 1 cut(s) 196
MflI RGATCY 1 cut(s) 524
MhlI GDGCHC 3 cut(s) 387, 703, 876
MluCI AATT 1 cut(s) 965
MlyI GAGTC 3 cut(s) 119, 458, 869
MmeI TCCRAC 1 cut(s) 837
MseI TTAA 1 cut(s) 960
MspA1I CMGCKG 1 cut(s) 823
MspI CCGG 3 cut(s) 664, 713, 886
MspR9I CCNGG 5 cut(s) 430, 443, 608, 665, 886
MvaI CCWGG 3 cut(s) 430, 443, 608
MvnI CGCG 6 cut(s) 521, 545, 555, 848, 867, 990
MwoI GCNNNNNNNGC 5 cut(s) 452, 542, 601, 810, 981
NciI CCSGG 2 cut(s) 665, 886
NdeII GATC 8 cut(s) 7, 130, 524, 579, 645, 724, 774, 843
NlaIII CATG 3 cut(s) 511, 911, 1027
NlaIV GGNNCC 4 cut(s) 526, 701, 818, 819
NmeAIII GCCGAG 1 cut(s) 964
NmuCI GTSAC 3 cut(s) 728, 861, 877
NsbI TGCGCA 1 cut(s) 985
PceI AGGCCT 1 cut(s) 446
PcsI WCGNNNNNNNCGW 1 cut(s) 411
PfeI GAWTC 2 cut(s) 125, 638
PflFI GACNNNGTC 1 cut(s) 410
PflMI CCANNNNNTGG 1 cut(s) 429
PkrI GCNGC 6 cut(s) 272, 275, 295, 400, 672, 850
PleI GAGTC 3 cut(s) 118, 458, 868
PmaCI CACGTG 1 cut(s) 877
PmlI CACGTG 1 cut(s) 877
PpsI GAGTC 3 cut(s) 118, 458, 868
Ppu21I YACGTR 1 cut(s) 877
PpuMI RGGWCCY 1 cut(s) 817
Psp5II RGGWCCY 1 cut(s) 817
Psp6I CCWGG 3 cut(s) 428, 441, 606
PspCI CACGTG 1 cut(s) 877
PspFI CCCAGC 1 cut(s) 343
PspGI CCWGG 3 cut(s) 428, 441, 606
PspN4I GGNNCC 4 cut(s) 526, 701, 818, 819
PspOMI GGGCCC 1 cut(s) 699
PspPI GGNCC 6 cut(s) 568, 633, 699, 700, 817, 883
PspPPI RGGWCCY 1 cut(s) 817
PsuI RGATCY 1 cut(s) 524
PsyI GACNNNGTC 1 cut(s) 410
RsaI GTAC 1 cut(s) 368
RsaNI GTAC 1 cut(s) 367
SaqAI TTAA 1 cut(s) 960
SatI GCNGC 6 cut(s) 271, 274, 294, 399, 671, 849
Sau3AI GATC 8 cut(s) 7, 130, 524, 579, 645, 724, 774, 843
Sau96I GGNCC 6 cut(s) 568, 633, 699, 700, 817, 883
SchI GAGTC 3 cut(s) 119, 458, 869
ScrFI CCNGG 5 cut(s) 430, 443, 608, 665, 886
SduI GDGCHC 3 cut(s) 387, 703, 876
SetI ASST 8 cut(s) 194, 246, 414, 431, 573, 653, 879, 921
SinI GGWCC 3 cut(s) 568, 633, 817
Sse9I AATT 1 cut(s) 965
SseBI AGGCCT 1 cut(s) 446
SspMI CTAG 2 cut(s) 350, 516
StuI AGGCCT 1 cut(s) 446
StyD4I CCNGG 5 cut(s) 428, 441, 606, 663, 884
StyI CCWWGG 1 cut(s) 159
TaaI ACNGT 3 cut(s) 371, 783, 855
TaiI ACGT 2 cut(s) 414, 879
TaqI TCGA 3 cut(s) 24, 174, 363
TaqII GACCGA 1 cut(s) 650
TasI AATT 1 cut(s) 965
TauI GCSGC 5 cut(s) 273, 276, 296, 401, 851
TfiI GAWTC 2 cut(s) 125, 638
Tru1I TTAA 1 cut(s) 960
Tru9I TTAA 1 cut(s) 960
TscAI CASTG 1 cut(s) 858
TseFI GTSAC 3 cut(s) 728, 861, 877
TseI GCWGC 1 cut(s) 670
Tsp45I GTSAC 3 cut(s) 728, 861, 877
TspDTI ATGAA 3 cut(s) 268, 493, 896
TspGWI ACGGA 2 cut(s) 349, 472
TspRI CASTG 1 cut(s) 858
Tth111I GACNNNGTC 1 cut(s) 410
Van91I CCANNNNNTGG 1 cut(s) 429
VneI GTGCAC 1 cut(s) 872
VpaK11BI GGWCC 3 cut(s) 568, 633, 817
XagI CCTNNNNNAGG 2 cut(s) 158, 713
XapI RAATTY 1 cut(s) 965
XmiI GTMKAC 1 cut(s) 550
XspI CTAG 2 cut(s) 350, 516
ZraI GACGTC 1 cut(s) 412
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.