Prupe.2G149600_v2.0.a1

carboxylesterase 8

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp02
OG0005485
Physical Location & Seq
Forward (+)
20507421 .. 20509572
2152 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.2G149600.1

Sequence Viewer

Length: 1059 bp
ATGGCGGATAAGCCTTCACAAACCCCAACATCCAACGACGACCCCTACAAGCTCCTCAACATCGTCAAAAACCCAGATGGCTCCCTCACCCGCCACCCCCCATTCCCCATAGTCCCCTCATCTCCGACTCCAACTGACTCCATTTCCTCCGCCGCAGCCACCCAGTCAAACTCACCCCAACTCGTTCTCTCCAAGGACATCCAAATAAACCCAACAACTAAAAACTCCGTAAGGATCTTCAAGCCCCACCCTCTTCCCGCAAACTCTAAGCTCCCTCTTATCTTCTACTTCCATGGCGGCGGCTTCGTCCTCTTCTCCGTGGCTTTACAACCTTTCCACGACTCCTGCTCTCGCATGGCGCTCTCTCTCCCGGCCCTCGTCGTCTCCGTCGAGTACCGCCTGGCTCCCGAGCACCCTCTCCCCTCTGCCTACGACGACGCCGTTGAGGCCGTCGACTGGGCCCGCAGCCAGGCCTCCAACGTCAACGGCTGCGATCCCTGGTTGAAGGACACCGTTGATTTCTCCAAGTGCTTCCTATTGGGCAGCAGCGCCGGGGCTAATATCGTCTACCACGCGGGTCTACGCATCGCGAGCGCTGATCTCTCTCCAGTGAAGATCCGAGGGCTAATATTGAACCAGCCTTATTTCGGTGGGGTCGAGAGGACTCAGTCCGAGTTAAGGCTGATCAACGATCGGATTTTGCCGTTGGTTTCTAATGATTTGATGTGGGCCCTGTCATTGCCGAGTGGAGCTGATCGGAATCATGAGTATAGCAATCCGACGGTACGGGGCGGTGATGAGAGGATCGGACAGTTGCCAACGTGTGTAGTGAGAGGGTTCGGTGGGGACCCACTGGTGGATAGGCAGAAGGAGTTTGTGAAGTTGTTGGAGTCACGTGGGGCGCATGTGGTGGCCAAGTTTGAAGAGGATGGTTTTCATGGAGTGGAGCTCTTTGATCCCAGCAAGGCTAAGGCTCTGTACGACATCGTTAAGGATTTAATCCATTCTTGCGGTGCAAACGACAGTAAAGCTGTTGGCGTTTCGAAATCAGCTATCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0009056 GO:0016787
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Abhydrolase_3

Protein Analysis

353

Amino Acids

38.13

Weight (kDa)

6.36

Isoelectric Point (pI)

40.99

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 3 cut(s) 453, 567, 580
AccII CGCG 2 cut(s) 575, 590
AclWI GGATC 5 cut(s) 242, 488, 610, 812, 950
AcoI YGGCCR 1 cut(s) 912
AcvI CACGTG 1 cut(s) 896
AcyI GRCGYC 1 cut(s) 438
AfaI GTAC 3 cut(s) 395, 786, 980
AfeI AGCGCT 1 cut(s) 595
AfiI CCNNNNNNNGG 5 cut(s) 456, 469, 647, 678, 856
AflIII ACRYGT 1 cut(s) 821
AgsI TTSAA 4 cut(s) 241, 505, 634, 923
AjnI CCWGG 3 cut(s) 399, 468, 497
AluBI AGCT 6 cut(s) 52, 271, 752, 949, 1031, 1052
AluI AGCT 6 cut(s) 52, 271, 752, 949, 1031, 1052
Alw21I GWGCWC 2 cut(s) 414, 951
Alw26I GTCTC 1 cut(s) 388
AlwI GGATC 5 cut(s) 242, 488, 610, 812, 950
Ama87I CYCGRG 1 cut(s) 407
Aor51HI AGCGCT 1 cut(s) 595
AoxI GGCC 6 cut(s) 372, 447, 459, 471, 729, 912
ApaI GGGCCC 2 cut(s) 463, 733
ApeKI GCWGC 5 cut(s) 155, 465, 489, 543, 546
AspLEI GCGC 4 cut(s) 361, 551, 596, 904
AspS9I GGNCC 6 cut(s) 373, 459, 460, 729, 730, 847
AsuC2I CCSGG 2 cut(s) 371, 553
AsuHPI GGTGA 3 cut(s) 79, 165, 806
AsuII TTCGAA 1 cut(s) 1043
AvaI CYCGRG 1 cut(s) 407
AvaII GGWCC 1 cut(s) 847
BaeGI GKGCMC 2 cut(s) 463, 733
BalI TGGCCA 1 cut(s) 914
BanII GRGCYC 3 cut(s) 463, 733, 951
BbrPI CACGTG 1 cut(s) 896
Bbv12I GWGCWC 2 cut(s) 414, 951
BbvI GCAGC 5 cut(s) 167, 476, 477, 555, 558
BccI CCATC 2 cut(s) 71, 923
BceAI ACGGC 4 cut(s) 425, 434, 502, 688
BciT130I CCWGG 3 cut(s) 401, 470, 499
BclI TGATCA 1 cut(s) 684
BcnI CCSGG 2 cut(s) 371, 553
BcoDI GTCTC 1 cut(s) 388
BfoI RGCGCY 3 cut(s) 362, 552, 597
BglI GCCNNNNNGGC 1 cut(s) 446
BisI GCNGC 8 cut(s) 153, 156, 298, 301, 466, 490, 544, 547
BlsI GCNGC 8 cut(s) 154, 157, 299, 302, 467, 491, 545, 548
Bme1390I CCNGG 5 cut(s) 371, 401, 470, 499, 553
Bme18I GGWCC 1 cut(s) 847
BmeT110I CYCGRG 1 cut(s) 407
BmgT120I GGNCC 6 cut(s) 373, 459, 460, 729, 730, 847
BmiI GGNNCC 6 cut(s) 82, 405, 461, 731, 848, 849
BmrFI CCNGG 5 cut(s) 371, 401, 470, 499, 553
BmrI ACTGGG 2 cut(s) 157, 466
BmsI GCATC 1 cut(s) 594
BmuI ACTGGG 2 cut(s) 157, 466
BplI GAGNNNNNCTC 2 cut(s) 933, 965
BpmI CTGGAG 1 cut(s) 591
Bpu10I CCTNAGC 1 cut(s) 969
Bpu14I TTCGAA 1 cut(s) 1043
BpuMI CCSGG 2 cut(s) 371, 553
BsaAI YACGTR 1 cut(s) 896
BsaBI GATNNNNATC 1 cut(s) 759
BsaHI GRCGYC 1 cut(s) 438
BsaJI CCNNGG 6 cut(s) 192, 292, 318, 497, 552, 619
Bsc4I CCNNNNNNNGG 5 cut(s) 456, 469, 647, 678, 856
Bse1I ACTGG 4 cut(s) 163, 461, 608, 858
Bse3DI GCAATG 1 cut(s) 737
Bse8I GATNNNNATC 1 cut(s) 759
BseBI CCWGG 3 cut(s) 401, 470, 499
BseDI CCNNGG 6 cut(s) 192, 292, 318, 497, 552, 619
BseGI GGATG 3 cut(s) 29, 198, 934
BseJI GATNNNNATC 1 cut(s) 759
BseLI CCNNNNNNNGG 5 cut(s) 456, 469, 647, 678, 856
BseMI GCAATG 1 cut(s) 737
BseMII CTCAG 1 cut(s) 680
BseNI ACTGG 4 cut(s) 163, 461, 608, 858
BseRI GAGGAG 1 cut(s) 44
BseSI GKGCMC 2 cut(s) 463, 733
BseXI GCAGC 5 cut(s) 167, 476, 477, 555, 558
BseYI CCCAGC 1 cut(s) 959
Bsh1236I CGCG 2 cut(s) 575, 590
Bsh1285I CGRYCG 1 cut(s) 694
BshFI GGCC 6 cut(s) 374, 449, 461, 473, 731, 914
BsiEI CGRYCG 1 cut(s) 694
BsiHKAI GWGCWC 2 cut(s) 414, 951
BsiHKCI CYCGRG 1 cut(s) 407
BsiSI CCGG 2 cut(s) 371, 552
BslFI GGGAC 2 cut(s) 98, 860
BslI CCNNNNNNNGG 5 cut(s) 456, 469, 647, 678, 856
BsmAI GTCTC 1 cut(s) 388
BsmBI CGTCTC 1 cut(s) 388
BsmFI GGGAC 2 cut(s) 98, 860
BsnI GGCC 6 cut(s) 374, 449, 461, 473, 731, 914
BsoBI CYCGRG 1 cut(s) 407
Bsp119I TTCGAA 1 cut(s) 1043
Bsp120I GGGCCC 2 cut(s) 459, 729
Bsp1286I GDGCHC 4 cut(s) 414, 463, 733, 951
Bsp143I GATC 9 cut(s) 234, 493, 598, 615, 684, 691, 754, 804, 955
Bsp19I CCATGG 1 cut(s) 292
Bsp68I TCGCGA 1 cut(s) 590
BspANI GGCC 6 cut(s) 374, 449, 461, 473, 731, 914
BspCNI CTCAG 1 cut(s) 679
BspFNI CGCG 2 cut(s) 575, 590
BspHI TCATGA 1 cut(s) 763
BspLI GGNNCC 6 cut(s) 82, 405, 461, 731, 848, 849
BspPI GGATC 5 cut(s) 242, 488, 610, 812, 950
BspT104I TTCGAA 1 cut(s) 1043
BsrDI GCAATG 1 cut(s) 737
BsrI ACTGG 4 cut(s) 163, 461, 608, 858
BssECI CCNNGG 6 cut(s) 192, 292, 318, 497, 552, 619
BssMI GATC 9 cut(s) 234, 493, 598, 615, 684, 691, 754, 804, 955
BssNI GRCGYC 1 cut(s) 438
BssT1I CCWWGG 2 cut(s) 192, 292
Bst2UI CCWGG 3 cut(s) 401, 470, 499
Bst4CI ACNGT 4 cut(s) 514, 784, 813, 1025
Bst6I CTCTTC 3 cut(s) 258, 317, 918
BstACI GRCGYC 1 cut(s) 438
BstBAI YACGTR 1 cut(s) 896
BstBI TTCGAA 1 cut(s) 1043
BstC8I GCNNGC 2 cut(s) 463, 592
BstDEI CTNAG 3 cut(s) 267, 666, 969
BstDSI CCRYGG 2 cut(s) 292, 318
BstF5I GGATG 3 cut(s) 29, 198, 934
BstFNI CGCG 2 cut(s) 575, 590
BstH2I RGCGCY 3 cut(s) 362, 552, 597
BstHHI GCGC 4 cut(s) 361, 551, 596, 904
BstKTI GATC 9 cut(s) 237, 496, 601, 618, 687, 694, 757, 807, 958
BstMAI GTCTC 1 cut(s) 388
BstMBI GATC 9 cut(s) 234, 493, 598, 615, 684, 691, 754, 804, 955
BstMCI CGRYCG 1 cut(s) 694
BstMWI GCNNNNNNNGC 2 cut(s) 446, 591
BstNI CCWGG 3 cut(s) 401, 470, 499
BstNSI RCATGY 1 cut(s) 908
BstSCI CCNGG 5 cut(s) 369, 399, 468, 497, 551
BstSLI GKGCMC 2 cut(s) 463, 733
BstUI CGCG 2 cut(s) 575, 590
BstV1I GCAGC 5 cut(s) 167, 476, 477, 555, 558
BstX2I RGATCY 2 cut(s) 234, 615
BstYI RGATCY 2 cut(s) 234, 615
BsuRI GGCC 6 cut(s) 374, 449, 461, 473, 731, 914
BtgI CCRYGG 2 cut(s) 292, 318
BtgZI GCGATG 1 cut(s) 571
BtsCI GGATG 3 cut(s) 29, 198, 934
BtsIMutI CAGTG 2 cut(s) 615, 851
BtuMI TCGCGA 1 cut(s) 590
Cac8I GCNNGC 2 cut(s) 463, 592
CciI TCATGA 1 cut(s) 763
CfoI GCGC 4 cut(s) 361, 551, 596, 904
Cfr13I GGNCC 6 cut(s) 373, 459, 460, 729, 730, 847
CseI GACGC 1 cut(s) 446
Csp6I GTAC 3 cut(s) 394, 785, 979
CviAII CATG 5 cut(s) 293, 355, 764, 905, 938
CviQI GTAC 3 cut(s) 394, 785, 979
DdeI CTNAG 3 cut(s) 267, 666, 969
DpnI GATC 9 cut(s) 236, 495, 600, 617, 686, 693, 756, 806, 957
DpnII GATC 9 cut(s) 234, 493, 598, 615, 684, 691, 754, 804, 955
EaeI YGGCCR 1 cut(s) 912
Eam1104I CTCTTC 3 cut(s) 258, 317, 918
EarI CTCTTC 3 cut(s) 258, 317, 918
EciI GGCGGA 2 cut(s) 20, 139
Ecl136II GAGCTC 1 cut(s) 949
Eco130I CCWWGG 2 cut(s) 192, 292
Eco147I AGGCCT 1 cut(s) 473
Eco24I GRGCYC 3 cut(s) 463, 733, 951
Eco47I GGWCC 1 cut(s) 847
Eco47III AGCGCT 1 cut(s) 595
Eco53kI GAGCTC 1 cut(s) 949
Eco72I CACGTG 1 cut(s) 896
Eco88I CYCGRG 1 cut(s) 407
EcoICRI GAGCTC 1 cut(s) 949
EcoO109I RGGNCCY 2 cut(s) 730, 847
EcoRII CCWGG 3 cut(s) 399, 468, 497
EcoT14I CCWWGG 2 cut(s) 192, 292
EcoT38I GRGCYC 3 cut(s) 463, 733, 951
ErhI CCWWGG 2 cut(s) 192, 292
Esp3I CGTCTC 1 cut(s) 388
FaeI CATG 5 cut(s) 296, 358, 767, 908, 941
FaiI YATR 7 cut(s) 110, 294, 356, 765, 771, 906, 939
FaqI GGGAC 2 cut(s) 98, 860
FatI CATG 5 cut(s) 292, 354, 763, 904, 937
FauI CCCGC 4 cut(s) 98, 265, 470, 568
FbaI TGATCA 1 cut(s) 684
FblI GTMKAC 3 cut(s) 453, 567, 580
Fnu4HI GCNGC 8 cut(s) 153, 156, 298, 301, 466, 490, 544, 547
FokI GGATG 3 cut(s) 16, 185, 941
FriOI GRGCYC 3 cut(s) 463, 733, 951
Fsp4HI GCNGC 8 cut(s) 153, 156, 298, 301, 466, 490, 544, 547
GlaI GCGC 4 cut(s) 360, 550, 595, 903
GluI GCNGC 8 cut(s) 153, 156, 298, 301, 466, 490, 544, 547
GsaI CCCAGC 1 cut(s) 963
GsuI CTGGAG 1 cut(s) 591
HaeII RGCGCY 3 cut(s) 362, 552, 597
HaeIII GGCC 6 cut(s) 374, 449, 461, 473, 731, 914
HapII CCGG 2 cut(s) 371, 552
HgaI GACGC 1 cut(s) 446
HhaI GCGC 4 cut(s) 361, 551, 596, 904
Hin1I GRCGYC 1 cut(s) 438
Hin1II CATG 5 cut(s) 296, 358, 767, 908, 941
Hin6I GCGC 4 cut(s) 359, 549, 594, 902
HinP1I GCGC 4 cut(s) 359, 549, 594, 902
HincII GTYRAC 2 cut(s) 454, 484
HindII GTYRAC 2 cut(s) 454, 484
HinfI GANTC 6 cut(s) 127, 137, 341, 664, 760, 890
HpaII CCGG 2 cut(s) 371, 552
HphI GGTGA 3 cut(s) 79, 165, 806
Hpy166II GTNNAC 4 cut(s) 454, 484, 568, 581
Hpy188I TCNGA 8 cut(s) 126, 620, 673, 696, 759, 780, 809, 1058
Hpy188III TCNNGA 4 cut(s) 407, 589, 658, 764
Hpy8I GTNNAC 4 cut(s) 454, 484, 568, 581
Hpy99I CGWCG 7 cut(s) 41, 383, 392, 437, 440, 455, 784
HpyAV CCTTC 3 cut(s) 24, 499, 862
HpyCH4III ACNGT 4 cut(s) 514, 784, 813, 1025
HpyCH4IV ACGT 3 cut(s) 480, 821, 895
HpyCH4V TGCA 1 cut(s) 1016
HpyF10VI GCNNNNNNNGC 2 cut(s) 446, 591
HpyF3I CTNAG 3 cut(s) 267, 666, 969
HpySE526I ACGT 3 cut(s) 480, 821, 895
Hsp92I GRCGYC 1 cut(s) 438
Hsp92II CATG 5 cut(s) 296, 358, 767, 908, 941
HspAI GCGC 4 cut(s) 359, 549, 594, 902
KflI GGGWCCC 1 cut(s) 847
Ksp22I TGATCA 1 cut(s) 684
Kzo9I GATC 9 cut(s) 234, 493, 598, 615, 684, 691, 754, 804, 955
LmnI GCTCC 6 cut(s) 57, 86, 276, 409, 749, 946
Lsp1109I GCAGC 5 cut(s) 167, 476, 477, 555, 558
LweI GCATC 1 cut(s) 594
MaeII ACGT 3 cut(s) 480, 821, 895
MaeIII GTNAC 1 cut(s) 891
MalI GATC 9 cut(s) 236, 495, 600, 617, 686, 693, 756, 806, 957
MboI GATC 9 cut(s) 234, 493, 598, 615, 684, 691, 754, 804, 955
MboII GAAGA 6 cut(s) 229, 245, 274, 304, 625, 935
MflI RGATCY 2 cut(s) 234, 615
MhlI GDGCHC 4 cut(s) 414, 463, 733, 951
MlsI TGGCCA 1 cut(s) 914
MluNI TGGCCA 1 cut(s) 914
MlyI GAGTC 5 cut(s) 121, 131, 335, 658, 899
MmeI TCCRAC 6 cut(s) 57, 149, 155, 501, 803, 867
Mox20I TGGCCA 1 cut(s) 914
MscI TGGCCA 1 cut(s) 914
MseI TTAA 3 cut(s) 677, 990, 998
Msp20I TGGCCA 1 cut(s) 914
MspI CCGG 2 cut(s) 371, 552
MspR9I CCNGG 5 cut(s) 371, 401, 470, 499, 553
MvaI CCWGG 3 cut(s) 401, 470, 499
MvnI CGCG 2 cut(s) 575, 590
MwoI GCNNNNNNNGC 2 cut(s) 446, 591
NciI CCSGG 2 cut(s) 371, 553
NcoI CCATGG 1 cut(s) 292
NdeII GATC 9 cut(s) 234, 493, 598, 615, 684, 691, 754, 804, 955
NlaIII CATG 5 cut(s) 296, 358, 767, 908, 941
NlaIV GGNNCC 6 cut(s) 82, 405, 461, 731, 848, 849
NmeAIII GCCGAG 1 cut(s) 768
NmuCI GTSAC 1 cut(s) 891
NruI TCGCGA 1 cut(s) 590
NspI RCATGY 1 cut(s) 908
NspV TTCGAA 1 cut(s) 1043
PagI TCATGA 1 cut(s) 763
PceI AGGCCT 1 cut(s) 473
PcsI WCGNNNNNNNCGW 4 cut(s) 384, 387, 438, 654
PfeI GAWTC 1 cut(s) 760
PflFI GACNNNGTC 1 cut(s) 667
PkrI GCNGC 8 cut(s) 154, 157, 299, 302, 467, 491, 545, 548
Ple19I CGATCG 1 cut(s) 694
PleI GAGTC 5 cut(s) 121, 131, 335, 658, 898
PmaCI CACGTG 1 cut(s) 896
PmlI CACGTG 1 cut(s) 896
PpsI GAGTC 5 cut(s) 121, 131, 335, 658, 898
Ppu21I YACGTR 1 cut(s) 896
PpuMI RGGWCCY 1 cut(s) 847
Psp124BI GAGCTC 1 cut(s) 951
Psp5II RGGWCCY 1 cut(s) 847
Psp6I CCWGG 3 cut(s) 399, 468, 497
PspCI CACGTG 1 cut(s) 896
PspFI CCCAGC 1 cut(s) 959
PspGI CCWGG 3 cut(s) 399, 468, 497
PspN4I GGNNCC 6 cut(s) 82, 405, 461, 731, 848, 849
PspOMI GGGCCC 2 cut(s) 459, 729
PspPI GGNCC 6 cut(s) 373, 459, 460, 729, 730, 847
PspPPI RGGWCCY 1 cut(s) 847
PsuI RGATCY 2 cut(s) 234, 615
PsyI GACNNNGTC 1 cut(s) 667
PvuI CGATCG 1 cut(s) 694
RruI TCGCGA 1 cut(s) 590
RsaI GTAC 3 cut(s) 395, 786, 980
RsaNI GTAC 3 cut(s) 394, 785, 979
SacI GAGCTC 1 cut(s) 951
SalI GTCGAC 1 cut(s) 452
SaqAI TTAA 3 cut(s) 677, 990, 998
SatI GCNGC 8 cut(s) 153, 156, 298, 301, 466, 490, 544, 547
Sau3AI GATC 9 cut(s) 234, 493, 598, 615, 684, 691, 754, 804, 955
Sau96I GGNCC 6 cut(s) 373, 459, 460, 729, 730, 847
SchI GAGTC 5 cut(s) 121, 131, 335, 658, 899
ScrFI CCNGG 5 cut(s) 371, 401, 470, 499, 553
SduI GDGCHC 4 cut(s) 414, 463, 733, 951
SfaNI GCATC 1 cut(s) 594
SfuI TTCGAA 1 cut(s) 1043
SinI GGWCC 1 cut(s) 847
SseBI AGGCCT 1 cut(s) 473
SspI AATATT 1 cut(s) 630
SstI GAGCTC 1 cut(s) 951
StuI AGGCCT 1 cut(s) 473
StyD4I CCNGG 5 cut(s) 369, 399, 468, 497, 551
StyI CCWWGG 2 cut(s) 192, 292
TaaI ACNGT 4 cut(s) 514, 784, 813, 1025
TaiI ACGT 3 cut(s) 483, 824, 898
TaqI TCGA 4 cut(s) 390, 453, 657, 1043
TauI GCSGC 3 cut(s) 155, 300, 303
TfiI GAWTC 1 cut(s) 760
Tru1I TTAA 3 cut(s) 677, 990, 998
Tru9I TTAA 3 cut(s) 677, 990, 998
TscAI CASTG 2 cut(s) 615, 858
TseFI GTSAC 1 cut(s) 891
TseI GCWGC 5 cut(s) 155, 465, 489, 543, 546
Tsp45I GTSAC 1 cut(s) 891
TspDTI ATGAA 1 cut(s) 926
TspGWI ACGGA 3 cut(s) 217, 307, 376
TspRI CASTG 2 cut(s) 615, 858
Tth111I GACNNNGTC 1 cut(s) 667
VpaK11BI GGWCC 1 cut(s) 847
XceI RCATGY 1 cut(s) 908
XmiI GTMKAC 3 cut(s) 453, 567, 580
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.