AT3G22231

Cysteine-rich and transmembrane domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0001954
Physical Location & Seq
Reverse (-)
7845823 .. 7846833
1011 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G22231.1

Sequence Viewer

Length: 246 bp
ATGAATCAATCCGCGCAAAATTACTTTTCCGTGCAAAAACCTTCAGAGACTTCATCAGGGCCGTACACAAGTCCGCCACCAATTGGTTATCCGACTAGAGATGCGGTGGTGGGTGATCCTCCGGCAGCAGCAGTGGAGACAAACTCCAAGGGCGTCAACCCCGAAGCCATAATGAGTTGTTTTAGTACTTGTATGGAGTGTATCTTCTGCTGCGGCGTATGCTCCAGCCTCTGTACATCAGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CYSTM

Protein Analysis

81

Amino Acids

8.48

Weight (kDa)

4.39

Isoelectric Point (pI)

60.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CYSTM PF12734 29 - 78 2.2e-07 Cysteine-rich TM module stress tolerance
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 83
AccII CGCG 1 cut(s) 14
AciI CCGC 4 cut(s) 12, 74, 104, 213
AclWI GGATC 1 cut(s) 110
AcuI CTGAAG 1 cut(s) 27
AcyI GRCGYC 1 cut(s) 153
AfaI GTAC 3 cut(s) 65, 187, 235
AfiI CCNNNNNNNGG 1 cut(s) 83
Alw26I GTCTC 2 cut(s) 41, 131
AlwI GGATC 1 cut(s) 110
AlwNI CAGNNNCTG 1 cut(s) 231
AoxI GGCC 1 cut(s) 59
ApeKI GCWGC 3 cut(s) 125, 128, 210
AspLEI GCGC 1 cut(s) 16
AspS9I GGNCC 1 cut(s) 59
AsuHPI GGTGA 1 cut(s) 125
BbvI GCAGC 3 cut(s) 137, 140, 197
BceAI ACGGC 1 cut(s) 46
BcoDI GTCTC 2 cut(s) 41, 131
BfaI CTAG 1 cut(s) 96
BisI GCNGC 4 cut(s) 126, 129, 211, 214
BlsI GCNGC 4 cut(s) 127, 130, 212, 215
BmcAI AGTACT 1 cut(s) 187
BmgT120I GGNCC 1 cut(s) 59
BmsI GCATC 1 cut(s) 91
BplI GAGNNNNNCTC 2 cut(s) 128, 160
BpmI CTGGAG 1 cut(s) 208
BsaHI GRCGYC 1 cut(s) 153
BsaJI CCNNGG 1 cut(s) 147
Bsc4I CCNNNNNNNGG 1 cut(s) 83
BseDI CCNNGG 1 cut(s) 147
BseLI CCNNNNNNNGG 1 cut(s) 83
BseXI GCAGC 3 cut(s) 137, 140, 197
Bsh1236I CGCG 1 cut(s) 14
BshFI GGCC 1 cut(s) 61
BsiSI CCGG 1 cut(s) 122
BslI CCNNNNNNNGG 1 cut(s) 83
BsmAI GTCTC 2 cut(s) 41, 131
BsnI GGCC 1 cut(s) 61
Bsp1407I TGTACA 1 cut(s) 233
Bsp143I GATC 1 cut(s) 115
BspACI CCGC 4 cut(s) 12, 74, 104, 213
BspANI GGCC 1 cut(s) 61
BspFNI CGCG 1 cut(s) 14
BspPI GGATC 1 cut(s) 110
BsrGI TGTACA 1 cut(s) 233
BssECI CCNNGG 1 cut(s) 147
BssMI GATC 1 cut(s) 115
BssNI GRCGYC 1 cut(s) 153
BssT1I CCWWGG 1 cut(s) 147
BstACI GRCGYC 1 cut(s) 153
BstAUI TGTACA 1 cut(s) 233
BstFNI CGCG 1 cut(s) 14
BstHHI GCGC 1 cut(s) 16
BstKTI GATC 1 cut(s) 118
BstMAI GTCTC 2 cut(s) 41, 131
BstMBI GATC 1 cut(s) 115
BstMWI GCNNNNNNNGC 1 cut(s) 219
BstUI CGCG 1 cut(s) 14
BstV1I GCAGC 3 cut(s) 137, 140, 197
BsuRI GGCC 1 cut(s) 61
BtsI GCAGTG 1 cut(s) 138
BtsIMutI CAGTG 1 cut(s) 138
CaiI CAGNNNCTG 1 cut(s) 231
CfoI GCGC 1 cut(s) 16
Cfr13I GGNCC 1 cut(s) 59
CseI GACGC 1 cut(s) 142
Csp6I GTAC 3 cut(s) 64, 186, 234
CviJI RGCY 3 cut(s) 61, 167, 228
CviKI_1 RGCY 3 cut(s) 61, 167, 228
CviQI GTAC 3 cut(s) 64, 186, 234
DpnI GATC 1 cut(s) 117
DpnII GATC 1 cut(s) 115
EciI GGCGGA 1 cut(s) 63
Eco130I CCWWGG 1 cut(s) 147
Eco57I CTGAAG 1 cut(s) 27
EcoT14I CCWWGG 1 cut(s) 147
ErhI CCWWGG 1 cut(s) 147
FaiI YATR 3 cut(s) 170, 194, 220
Fnu4HI GCNGC 4 cut(s) 126, 129, 211, 214
Fsp4HI GCNGC 4 cut(s) 126, 129, 211, 214
FspBI CTAG 1 cut(s) 96
GlaI GCGC 1 cut(s) 15
GluI GCNGC 4 cut(s) 126, 129, 211, 214
GsuI CTGGAG 1 cut(s) 208
HaeIII GGCC 1 cut(s) 61
HapII CCGG 1 cut(s) 122
HgaI GACGC 1 cut(s) 142
HhaI GCGC 1 cut(s) 16
Hin1I GRCGYC 1 cut(s) 153
Hin6I GCGC 1 cut(s) 14
HinP1I GCGC 1 cut(s) 14
HincII GTYRAC 1 cut(s) 157
HindII GTYRAC 1 cut(s) 157
HinfI GANTC 1 cut(s) 4
HpaII CCGG 1 cut(s) 122
HphI GGTGA 1 cut(s) 125
Hpy166II GTNNAC 2 cut(s) 66, 157
Hpy188I TCNGA 3 cut(s) 46, 93, 241
Hpy8I GTNNAC 2 cut(s) 66, 157
HpyAV CCTTC 1 cut(s) 51
HpyCH4V TGCA 1 cut(s) 34
HpyF10VI GCNNNNNNNGC 1 cut(s) 219
Hsp92I GRCGYC 1 cut(s) 153
HspAI GCGC 1 cut(s) 14
Kzo9I GATC 1 cut(s) 115
LmnI GCTCC 1 cut(s) 227
LpnPI CCDG 3 cut(s) 42, 135, 238
Lsp1109I GCAGC 3 cut(s) 137, 140, 197
LweI GCATC 1 cut(s) 91
MaeI CTAG 1 cut(s) 96
MalI GATC 1 cut(s) 117
MboI GATC 1 cut(s) 115
MboII GAAGA 1 cut(s) 196
MfeI CAATTG 1 cut(s) 81
MluCI AATT 2 cut(s) 19, 81
MmeI TCCRAC 1 cut(s) 116
MnlI CCTC 2 cut(s) 129, 239
MspI CCGG 1 cut(s) 122
MunI CAATTG 1 cut(s) 81
MvnI CGCG 1 cut(s) 14
MwoI GCNNNNNNNGC 1 cut(s) 219
NdeII GATC 1 cut(s) 115
PfeI GAWTC 1 cut(s) 4
PflMI CCANNNNNTGG 1 cut(s) 83
PkrI GCNGC 4 cut(s) 127, 130, 212, 215
PspPI GGNCC 1 cut(s) 59
PstNI CAGNNNCTG 1 cut(s) 231
RsaI GTAC 3 cut(s) 65, 187, 235
RsaNI GTAC 3 cut(s) 64, 186, 234
SatI GCNGC 4 cut(s) 126, 129, 211, 214
Sau3AI GATC 1 cut(s) 115
Sau96I GGNCC 1 cut(s) 59
ScaI AGTACT 1 cut(s) 187
SetI ASST 1 cut(s) 43
SfaNI GCATC 1 cut(s) 91
Sse9I AATT 2 cut(s) 19, 81
SsiI CCGC 4 cut(s) 12, 74, 104, 213
SspMI CTAG 1 cut(s) 96
StyI CCWWGG 1 cut(s) 147
TasI AATT 2 cut(s) 19, 81
TatI WGTACW 2 cut(s) 185, 233
TauI GCSGC 1 cut(s) 216
TfiI GAWTC 1 cut(s) 4
TscAI CASTG 1 cut(s) 138
TseI GCWGC 3 cut(s) 125, 128, 210
TspDTI ATGAA 2 cut(s) 17, 42
TspGWI ACGGA 1 cut(s) 19
TspRI CASTG 1 cut(s) 138
Van91I CCANNNNNTGG 1 cut(s) 83
XspI CTAG 1 cut(s) 96
ZrmI AGTACT 1 cut(s) 187
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.