AT3G22235

Cysteine-rich and transmembrane domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0001954
Physical Location & Seq
Reverse (-)
7854419 .. 7855814
1396 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G22235.1

Sequence Viewer

Length: 216 bp
ATGAATCAATCCGCGCAAAATTACTTTTCCGTGCAAAAACCTTCAGAGACTTCATCAGGGCCGTACACAAGTCCGCCACCAATTGGTTATCCGACTAGAGATGCGGTGGTGGGTGATCCTCCGGCAGCAGCAGTGGAGACAAACTCCAAGGGCGTCAACCCCGAAGGATGTTGTGCTGCCATATGCTGCTGTTGTGTCCTGGATGCATGCTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CYSTM

Protein Analysis

71

Amino Acids

7.32

Weight (kDa)

4.3

Isoelectric Point (pI)

41.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CYSTM PF12734 29 - 71 2e-07 Cysteine-rich TM module stress tolerance
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 83
AccII CGCG 1 cut(s) 14
AciI CCGC 3 cut(s) 12, 74, 104
AclWI GGATC 1 cut(s) 110
AcuI CTGAAG 1 cut(s) 27
AcyI GRCGYC 1 cut(s) 153
AfaI GTAC 1 cut(s) 65
AfiI CCNNNNNNNGG 1 cut(s) 83
AjnI CCWGG 1 cut(s) 198
Alw26I GTCTC 2 cut(s) 41, 131
AlwI GGATC 1 cut(s) 110
AoxI GGCC 1 cut(s) 59
ApeKI GCWGC 4 cut(s) 125, 128, 176, 186
AspLEI GCGC 1 cut(s) 16
AspS9I GGNCC 1 cut(s) 59
AsuHPI GGTGA 1 cut(s) 125
BbvI GCAGC 4 cut(s) 137, 140, 163, 173
BceAI ACGGC 1 cut(s) 46
BciT130I CCWGG 1 cut(s) 200
BcoDI GTCTC 2 cut(s) 41, 131
BfaI CTAG 1 cut(s) 96
BisI GCNGC 4 cut(s) 126, 129, 177, 187
BlsI GCNGC 4 cut(s) 127, 130, 178, 188
Bme1390I CCNGG 1 cut(s) 200
BmgT120I GGNCC 1 cut(s) 59
BmrFI CCNGG 1 cut(s) 200
BmsI GCATC 2 cut(s) 91, 193
BplI GAGNNNNNCTC 2 cut(s) 128, 160
BsaHI GRCGYC 1 cut(s) 153
BsaJI CCNNGG 1 cut(s) 147
Bsc4I CCNNNNNNNGG 1 cut(s) 83
BseBI CCWGG 1 cut(s) 200
BseDI CCNNGG 1 cut(s) 147
BseGI GGATG 2 cut(s) 173, 208
BseLI CCNNNNNNNGG 1 cut(s) 83
BseXI GCAGC 4 cut(s) 137, 140, 163, 173
Bsh1236I CGCG 1 cut(s) 14
BshFI GGCC 1 cut(s) 61
BsiSI CCGG 1 cut(s) 122
BslI CCNNNNNNNGG 1 cut(s) 83
BsmAI GTCTC 2 cut(s) 41, 131
BsnI GGCC 1 cut(s) 61
Bsp143I GATC 1 cut(s) 115
BspACI CCGC 3 cut(s) 12, 74, 104
BspANI GGCC 1 cut(s) 61
BspFNI CGCG 1 cut(s) 14
BspPI GGATC 1 cut(s) 110
BssECI CCNNGG 1 cut(s) 147
BssMI GATC 1 cut(s) 115
BssNI GRCGYC 1 cut(s) 153
BssT1I CCWWGG 1 cut(s) 147
Bst2UI CCWGG 1 cut(s) 200
BstACI GRCGYC 1 cut(s) 153
BstC8I GCNNGC 1 cut(s) 208
BstF5I GGATG 2 cut(s) 173, 208
BstFNI CGCG 1 cut(s) 14
BstHHI GCGC 1 cut(s) 16
BstKTI GATC 1 cut(s) 118
BstMAI GTCTC 2 cut(s) 41, 131
BstMBI GATC 1 cut(s) 115
BstNI CCWGG 1 cut(s) 200
BstNSI RCATGY 1 cut(s) 210
BstSCI CCNGG 1 cut(s) 198
BstUI CGCG 1 cut(s) 14
BstV1I GCAGC 4 cut(s) 137, 140, 163, 173
BsuRI GGCC 1 cut(s) 61
BtsCI GGATG 2 cut(s) 173, 208
BtsI GCAGTG 1 cut(s) 138
BtsIMutI CAGTG 1 cut(s) 138
Cac8I GCNNGC 1 cut(s) 208
CfoI GCGC 1 cut(s) 16
Cfr13I GGNCC 1 cut(s) 59
CseI GACGC 1 cut(s) 142
Csp6I GTAC 1 cut(s) 64
CviAII CATG 1 cut(s) 207
CviJI RGCY 1 cut(s) 61
CviKI_1 RGCY 1 cut(s) 61
CviQI GTAC 1 cut(s) 64
DpnI GATC 1 cut(s) 117
DpnII GATC 1 cut(s) 115
EciI GGCGGA 1 cut(s) 63
Eco130I CCWWGG 1 cut(s) 147
Eco57I CTGAAG 1 cut(s) 27
EcoRII CCWGG 1 cut(s) 198
EcoT14I CCWWGG 1 cut(s) 147
EcoT22I ATGCAT 1 cut(s) 208
ErhI CCWWGG 1 cut(s) 147
FaeI CATG 1 cut(s) 210
FaiI YATR 3 cut(s) 182, 184, 208
FatI CATG 1 cut(s) 206
FauNDI CATATG 1 cut(s) 182
Fnu4HI GCNGC 4 cut(s) 126, 129, 177, 187
FokI GGATG 1 cut(s) 180
Fsp4HI GCNGC 4 cut(s) 126, 129, 177, 187
FspBI CTAG 1 cut(s) 96
GlaI GCGC 1 cut(s) 15
GluI GCNGC 4 cut(s) 126, 129, 177, 187
HaeIII GGCC 1 cut(s) 61
HapII CCGG 1 cut(s) 122
HgaI GACGC 1 cut(s) 142
HhaI GCGC 1 cut(s) 16
Hin1I GRCGYC 1 cut(s) 153
Hin1II CATG 1 cut(s) 210
Hin6I GCGC 1 cut(s) 14
HinP1I GCGC 1 cut(s) 14
HincII GTYRAC 1 cut(s) 157
HindII GTYRAC 1 cut(s) 157
HinfI GANTC 1 cut(s) 4
HpaII CCGG 1 cut(s) 122
HphI GGTGA 1 cut(s) 125
Hpy166II GTNNAC 2 cut(s) 66, 157
Hpy188I TCNGA 3 cut(s) 46, 93, 215
Hpy8I GTNNAC 2 cut(s) 66, 157
HpyAV CCTTC 2 cut(s) 51, 158
HpyCH4V TGCA 2 cut(s) 34, 206
Hsp92I GRCGYC 1 cut(s) 153
Hsp92II CATG 1 cut(s) 210
HspAI GCGC 1 cut(s) 14
Kzo9I GATC 1 cut(s) 115
LpnPI CCDG 4 cut(s) 42, 135, 185, 212
Lsp1109I GCAGC 4 cut(s) 137, 140, 163, 173
LweI GCATC 2 cut(s) 91, 193
MaeI CTAG 1 cut(s) 96
MalI GATC 1 cut(s) 117
MboI GATC 1 cut(s) 115
MfeI CAATTG 1 cut(s) 81
MluCI AATT 2 cut(s) 19, 81
MmeI TCCRAC 1 cut(s) 116
MnlI CCTC 1 cut(s) 129
Mph1103I ATGCAT 1 cut(s) 208
MspI CCGG 1 cut(s) 122
MspR9I CCNGG 1 cut(s) 200
MunI CAATTG 1 cut(s) 81
MvaI CCWGG 1 cut(s) 200
MvnI CGCG 1 cut(s) 14
NdeI CATATG 1 cut(s) 182
NdeII GATC 1 cut(s) 115
NlaIII CATG 1 cut(s) 210
NsiI ATGCAT 1 cut(s) 208
NspI RCATGY 1 cut(s) 210
PaeI GCATGC 1 cut(s) 210
PfeI GAWTC 1 cut(s) 4
PflMI CCANNNNNTGG 1 cut(s) 83
PfoI TCCNGGA 1 cut(s) 198
PkrI GCNGC 4 cut(s) 127, 130, 178, 188
Psp6I CCWGG 1 cut(s) 198
PspGI CCWGG 1 cut(s) 198
PspPI GGNCC 1 cut(s) 59
RsaI GTAC 1 cut(s) 65
RsaNI GTAC 1 cut(s) 64
SatI GCNGC 4 cut(s) 126, 129, 177, 187
Sau3AI GATC 1 cut(s) 115
Sau96I GGNCC 1 cut(s) 59
ScrFI CCNGG 1 cut(s) 200
SetI ASST 1 cut(s) 43
SfaNI GCATC 2 cut(s) 91, 193
SphI GCATGC 1 cut(s) 210
Sse9I AATT 2 cut(s) 19, 81
SsiI CCGC 3 cut(s) 12, 74, 104
SspMI CTAG 1 cut(s) 96
StyD4I CCNGG 1 cut(s) 198
StyI CCWWGG 1 cut(s) 147
TasI AATT 2 cut(s) 19, 81
TfiI GAWTC 1 cut(s) 4
TscAI CASTG 1 cut(s) 138
TseI GCWGC 4 cut(s) 125, 128, 176, 186
TspDTI ATGAA 2 cut(s) 17, 42
TspGWI ACGGA 1 cut(s) 19
TspRI CASTG 1 cut(s) 138
Van91I CCANNNNNTGG 1 cut(s) 83
XceI RCATGY 1 cut(s) 210
XspI CTAG 1 cut(s) 96
Zsp2I ATGCAT 1 cut(s) 208
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.