MD09G1137600.v1.1

CYSTM1 family protein A-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0001954
Physical Location & Seq
Forward (+)
10697041 .. 10698357
1317 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1137600.v1.1.491

Sequence Viewer

Length: 216 bp
ATGAGTACTACTAATTCAGCAGCAGGACACCCCGCTCCAGCTTCCGGCGCCGCTCAGGGTCCCTATTACGTGCAAGCTCCGCCACCAGCCGGCTACCCAACAAGAGATGAACCCCAAAACGCGGTTCCGGTAGAAACCAAGTCAAAGGGTGATGGCTTCTGGAAGGGCTGCTGTGCTGCCCTCTGTTGCTGTTGCATGGTGGATGCATGTTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CYSTM

Protein Analysis

72

Amino Acids

7.26

Weight (kDa)

5.5

Isoelectric Point (pI)

50.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CYSTM PF12734 22 - 71 2.7e-10 Cysteine-rich TM module stress tolerance
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 47
AccBSI CCGCTC 2 cut(s) 35, 53
AccII CGCG 1 cut(s) 122
AciI CCGC 4 cut(s) 33, 51, 80, 122
AcyI GRCGYC 1 cut(s) 48
AfaI GTAC 1 cut(s) 7
AfiI CCNNNNNNNGG 3 cut(s) 44, 89, 121
AjuI GAANNNNNNNTTGG 2 cut(s) 108, 140
AluBI AGCT 2 cut(s) 41, 77
AluI AGCT 2 cut(s) 41, 77
ApeKI GCWGC 3 cut(s) 20, 168, 176
AspLEI GCGC 1 cut(s) 50
AspS9I GGNCC 1 cut(s) 59
AsuHPI GGTGA 1 cut(s) 161
AvaII GGWCC 1 cut(s) 59
BanI GGYRCC 1 cut(s) 47
BbvI GCAGC 3 cut(s) 32, 155, 163
BccI CCATC 1 cut(s) 146
BfoI RGCGCY 1 cut(s) 51
BisI GCNGC 4 cut(s) 21, 51, 169, 177
BlsI GCNGC 4 cut(s) 22, 52, 170, 178
BmcAI AGTACT 1 cut(s) 7
Bme18I GGWCC 1 cut(s) 59
BmgT120I GGNCC 1 cut(s) 59
BmiI GGNNCC 4 cut(s) 49, 60, 61, 126
BmsI GCATC 1 cut(s) 193
BpmI CTGGAG 1 cut(s) 21
Bpu10I CCTNAGC 1 cut(s) 54
BsaAI YACGTR 1 cut(s) 70
BsaHI GRCGYC 1 cut(s) 48
BsaWI WCCGGW 1 cut(s) 127
Bsc4I CCNNNNNNNGG 3 cut(s) 44, 89, 121
Bse118I RCCGGY 1 cut(s) 89
BseGI GGATG 1 cut(s) 208
BseLI CCNNNNNNNGG 3 cut(s) 44, 89, 121
BseMII CTCAG 1 cut(s) 68
BseXI GCAGC 3 cut(s) 32, 155, 163
Bsh1236I CGCG 1 cut(s) 122
BshNI GGYRCC 1 cut(s) 47
BsiSI CCGG 3 cut(s) 45, 90, 128
BslFI GGGAC 1 cut(s) 45
BslI CCNNNNNNNGG 3 cut(s) 44, 89, 121
BsmFI GGGAC 1 cut(s) 45
BspACI CCGC 4 cut(s) 33, 51, 80, 122
BspCNI CTCAG 1 cut(s) 67
BspFNI CGCG 1 cut(s) 122
BspLI GGNNCC 4 cut(s) 49, 60, 61, 126
BspT107I GGYRCC 1 cut(s) 47
BsrBI CCGCTC 2 cut(s) 35, 53
BsrFI RCCGGY 1 cut(s) 89
BssAI RCCGGY 1 cut(s) 89
BssNI GRCGYC 1 cut(s) 48
BstACI GRCGYC 1 cut(s) 48
BstBAI YACGTR 1 cut(s) 70
BstC8I GCNNGC 2 cut(s) 75, 91
BstDEI CTNAG 1 cut(s) 54
BstF5I GGATG 1 cut(s) 208
BstFNI CGCG 1 cut(s) 122
BstH2I RGCGCY 1 cut(s) 51
BstHHI GCGC 1 cut(s) 50
BstMWI GCNNNNNNNGC 2 cut(s) 47, 79
BstNSI RCATGY 1 cut(s) 210
BstUI CGCG 1 cut(s) 122
BstV1I GCAGC 3 cut(s) 32, 155, 163
BtsCI GGATG 1 cut(s) 208
Cac8I GCNNGC 2 cut(s) 75, 91
CfoI GCGC 1 cut(s) 50
Cfr10I RCCGGY 1 cut(s) 89
Cfr13I GGNCC 1 cut(s) 59
Csp6I GTAC 1 cut(s) 6
CviAII CATG 2 cut(s) 196, 207
CviJI RGCY 6 cut(s) 41, 77, 89, 93, 156, 168
CviKI_1 RGCY 6 cut(s) 41, 77, 89, 93, 156, 168
CviQI GTAC 1 cut(s) 6
DdeI CTNAG 1 cut(s) 54
DinI GGCGCC 1 cut(s) 49
EciI GGCGGA 1 cut(s) 69
Eco47I GGWCC 1 cut(s) 59
EcoO109I RGGNCCY 1 cut(s) 59
EcoT22I ATGCAT 1 cut(s) 208
EgeI GGCGCC 1 cut(s) 49
EheI GGCGCC 1 cut(s) 49
FaeI CATG 2 cut(s) 199, 210
FaiI YATR 2 cut(s) 197, 208
FaqI GGGAC 1 cut(s) 45
FatI CATG 2 cut(s) 195, 206
FauI CCCGC 1 cut(s) 40
Fnu4HI GCNGC 4 cut(s) 21, 51, 169, 177
Fsp4HI GCNGC 4 cut(s) 21, 51, 169, 177
GlaI GCGC 1 cut(s) 49
GluI GCNGC 4 cut(s) 21, 51, 169, 177
GsuI CTGGAG 1 cut(s) 21
HaeII RGCGCY 1 cut(s) 51
HapII CCGG 3 cut(s) 45, 90, 128
HhaI GCGC 1 cut(s) 50
Hin1I GRCGYC 1 cut(s) 48
Hin1II CATG 2 cut(s) 199, 210
Hin6I GCGC 1 cut(s) 48
HinP1I GCGC 1 cut(s) 48
HpaII CCGG 3 cut(s) 45, 90, 128
HphI GGTGA 1 cut(s) 161
Hpy188III TCNNGA 1 cut(s) 160
HpyAV CCTTC 1 cut(s) 157
HpyCH4IV ACGT 1 cut(s) 69
HpyCH4V TGCA 3 cut(s) 73, 195, 206
HpyF10VI GCNNNNNNNGC 2 cut(s) 47, 79
HpyF3I CTNAG 1 cut(s) 54
HpySE526I ACGT 1 cut(s) 69
Hsp92I GRCGYC 1 cut(s) 48
Hsp92II CATG 2 cut(s) 199, 210
HspAI GCGC 1 cut(s) 48
KasI GGCGCC 1 cut(s) 47
KflI GGGWCCC 1 cut(s) 59
KroI GCCGGC 1 cut(s) 89
KroNI GCCGGC 1 cut(s) 91
LmnI GCTCC 2 cut(s) 40, 82
LpnPI CCDG 8 cut(s) 9, 41, 51, 58, 99, 103, 141, 145
Lsp1109I GCAGC 3 cut(s) 32, 155, 163
LweI GCATC 1 cut(s) 193
MaeII ACGT 1 cut(s) 69
MbiI CCGCTC 2 cut(s) 35, 53
MluCI AATT 1 cut(s) 13
Mly113I GGCGCC 1 cut(s) 48
MnlI CCTC 1 cut(s) 191
Mph1103I ATGCAT 1 cut(s) 208
MroNI GCCGGC 1 cut(s) 89
MspI CCGG 3 cut(s) 45, 90, 128
MvnI CGCG 1 cut(s) 122
MwoI GCNNNNNNNGC 2 cut(s) 47, 79
NaeI GCCGGC 1 cut(s) 91
NarI GGCGCC 1 cut(s) 48
NgoMIV GCCGGC 1 cut(s) 89
NlaIII CATG 2 cut(s) 199, 210
NlaIV GGNNCC 4 cut(s) 49, 60, 61, 126
NsiI ATGCAT 1 cut(s) 208
NspI RCATGY 1 cut(s) 210
PdiI GCCGGC 1 cut(s) 91
PkrI GCNGC 4 cut(s) 22, 52, 170, 178
PluTI GGCGCC 1 cut(s) 51
Ppu21I YACGTR 1 cut(s) 70
PpuMI RGGWCCY 1 cut(s) 59
Psp5II RGGWCCY 1 cut(s) 59
PspN4I GGNNCC 4 cut(s) 49, 60, 61, 126
PspPI GGNCC 1 cut(s) 59
PspPPI RGGWCCY 1 cut(s) 59
RsaI GTAC 1 cut(s) 7
RsaNI GTAC 1 cut(s) 6
SatI GCNGC 4 cut(s) 21, 51, 169, 177
Sau96I GGNCC 1 cut(s) 59
ScaI AGTACT 1 cut(s) 7
SetI ASST 3 cut(s) 43, 72, 79
SfaNI GCATC 1 cut(s) 193
SfoI GGCGCC 1 cut(s) 49
SinI GGWCC 1 cut(s) 59
Sse9I AATT 1 cut(s) 13
SsiI CCGC 4 cut(s) 33, 51, 80, 122
SspDI GGCGCC 1 cut(s) 47
TaiI ACGT 1 cut(s) 72
TasI AATT 1 cut(s) 13
TatI WGTACW 1 cut(s) 5
TauI GCSGC 1 cut(s) 53
TseI GCWGC 3 cut(s) 20, 168, 176
TspDTI ATGAA 1 cut(s) 123
VpaK11BI GGWCC 1 cut(s) 59
XceI RCATGY 1 cut(s) 210
ZrmI AGTACT 1 cut(s) 7
Zsp2I ATGCAT 1 cut(s) 208
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.