RLG00000020722

CYSTM1 family protein A-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0001954
Physical Location & Seq
Reverse (-)
67957328 .. 67959430
2103 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000020722

Sequence Viewer

Length: 384 bp
ATGAGGTCCGGGATGTTGGGGAGAGGCGGCAATGATGACGAAGTCGACCAGTTTTTAAATGAGGATTTGCCTGATGAGTACAAGGGGATGGGGAAGGGAAGCATGGGTTGGACTTTGGTTGGTCTAACAAAGTCTGATTGGGAGAAGGATTCACCGGCGACGGTGGTGACCAGGTGTGCTAACTCCGCAGCAGCTTATCCCGCTCCGGCTTCCCAACAGGGACGTTACAACCAGGCGGCGCCTCCAGCCGGCTATCCCACCATGACAGACGATCAGTTTCCCCGAAGCCACGGTCAAGTCGAGACCAAGTCGAAGGGTGATGACTTTTGGAAGGGTTGCTGTGCTGCTCTTTGTTGCTGTTGTATGTTGGATGCTTGCTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CYSTM

Protein Analysis

128

Amino Acids

13.69

Weight (kDa)

4.7

Isoelectric Point (pI)

34.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CYSTM PF12734 89 - 127 2.7e-10 Cysteine-rich TM module stress tolerance
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 238
AccBSI CCGCTC 1 cut(s) 203
AccI GTMKAC 1 cut(s) 45
AciI CCGC 4 cut(s) 27, 186, 201, 236
AcyI GRCGYC 1 cut(s) 239
AfaI GTAC 1 cut(s) 80
AfiI CCNNNNNNNGG 1 cut(s) 248
AjnI CCWGG 2 cut(s) 170, 231
AjuI GAANNNNNNNTTGG 2 cut(s) 91, 123
AluBI AGCT 1 cut(s) 194
AluI AGCT 1 cut(s) 194
Alw26I GTCTC 1 cut(s) 296
ApeKI GCWGC 3 cut(s) 188, 191, 344
ArsI GACNNNNNNTTYG 2 cut(s) 277, 309
AspLEI GCGC 1 cut(s) 241
AspS9I GGNCC 1 cut(s) 6
AsuC2I CCSGG 1 cut(s) 10
AsuHPI GGTGA 3 cut(s) 144, 178, 329
AvaII GGWCC 1 cut(s) 6
BanI GGYRCC 1 cut(s) 238
BbvI GCAGC 3 cut(s) 200, 203, 331
BccI CCATC 1 cut(s) 82
BciT130I CCWGG 2 cut(s) 172, 233
BcnI CCSGG 1 cut(s) 10
BcoDI GTCTC 1 cut(s) 296
BfoI RGCGCY 1 cut(s) 242
BisI GCNGC 5 cut(s) 28, 189, 192, 237, 345
BlsI GCNGC 5 cut(s) 29, 190, 193, 238, 346
Bme1390I CCNGG 3 cut(s) 10, 172, 233
Bme18I GGWCC 1 cut(s) 6
BmgT120I GGNCC 1 cut(s) 6
BmiI GGNNCC 1 cut(s) 240
BmrFI CCNGG 3 cut(s) 10, 172, 233
BmsI GCATC 1 cut(s) 361
BpmI CTGGAG 1 cut(s) 228
BpuMI CCSGG 1 cut(s) 10
BsaHI GRCGYC 1 cut(s) 239
BsaI GGTCTC 1 cut(s) 296
BsaJI CCNNGG 1 cut(s) 289
Bsc4I CCNNNNNNNGG 1 cut(s) 248
Bse118I RCCGGY 2 cut(s) 154, 248
Bse1I ACTGG 1 cut(s) 49
Bse3DI GCAATG 1 cut(s) 37
BseBI CCWGG 2 cut(s) 172, 233
BseDI CCNNGG 1 cut(s) 289
BseGI GGATG 3 cut(s) 18, 93, 376
BseLI CCNNNNNNNGG 1 cut(s) 248
BseMI GCAATG 1 cut(s) 37
BseNI ACTGG 1 cut(s) 49
BseXI GCAGC 3 cut(s) 200, 203, 331
BshNI GGYRCC 1 cut(s) 238
BsiSI CCGG 4 cut(s) 9, 155, 206, 249
BslFI GGGAC 1 cut(s) 234
BslI CCNNNNNNNGG 1 cut(s) 248
BsmAI GTCTC 1 cut(s) 296
BsmFI GGGAC 1 cut(s) 234
Bso31I GGTCTC 1 cut(s) 296
Bsp143I GATC 1 cut(s) 271
BspACI CCGC 4 cut(s) 27, 186, 201, 236
BspLI GGNNCC 1 cut(s) 240
BspT107I GGYRCC 1 cut(s) 238
BspTNI GGTCTC 1 cut(s) 296
BsrBI CCGCTC 1 cut(s) 203
BsrDI GCAATG 1 cut(s) 37
BsrFI RCCGGY 2 cut(s) 154, 248
BsrI ACTGG 1 cut(s) 49
BssAI RCCGGY 2 cut(s) 154, 248
BssECI CCNNGG 1 cut(s) 289
BssMI GATC 1 cut(s) 271
BssNI GRCGYC 1 cut(s) 239
Bst2UI CCWGG 2 cut(s) 172, 233
Bst4CI ACNGT 2 cut(s) 163, 293
BstACI GRCGYC 1 cut(s) 239
BstC8I GCNNGC 2 cut(s) 250, 376
BstDSI CCRYGG 1 cut(s) 289
BstEII GGTNACC 1 cut(s) 166
BstF5I GGATG 3 cut(s) 18, 93, 376
BstH2I RGCGCY 1 cut(s) 242
BstHHI GCGC 1 cut(s) 241
BstKTI GATC 1 cut(s) 274
BstMAI GTCTC 1 cut(s) 296
BstMBI GATC 1 cut(s) 271
BstMWI GCNNNNNNNGC 3 cut(s) 185, 200, 245
BstNI CCWGG 2 cut(s) 172, 233
BstPI GGTNACC 1 cut(s) 166
BstSCI CCNGG 3 cut(s) 8, 170, 231
BstV1I GCAGC 3 cut(s) 200, 203, 331
BtgI CCRYGG 1 cut(s) 289
BtsCI GGATG 3 cut(s) 18, 93, 376
Cac8I GCNNGC 2 cut(s) 250, 376
CfoI GCGC 1 cut(s) 241
Cfr10I RCCGGY 2 cut(s) 154, 248
Cfr13I GGNCC 1 cut(s) 6
CsiI ACCWGGT 1 cut(s) 170
Csp6I GTAC 1 cut(s) 79
CviAII CATG 2 cut(s) 103, 262
CviJI RGCY 5 cut(s) 194, 209, 248, 252, 288
CviKI_1 RGCY 5 cut(s) 194, 209, 248, 252, 288
CviQI GTAC 1 cut(s) 79
DinI GGCGCC 1 cut(s) 240
DpnI GATC 1 cut(s) 273
DpnII GATC 1 cut(s) 271
DraI TTTAAA 1 cut(s) 57
Eco31I GGTCTC 1 cut(s) 296
Eco47I GGWCC 1 cut(s) 6
Eco91I GGTNACC 1 cut(s) 166
EcoO65I GGTNACC 1 cut(s) 166
EcoRII CCWGG 2 cut(s) 170, 231
EgeI GGCGCC 1 cut(s) 240
EheI GGCGCC 1 cut(s) 240
FaeI CATG 2 cut(s) 106, 265
FaiI YATR 3 cut(s) 104, 263, 365
FaqI GGGAC 1 cut(s) 234
FatI CATG 2 cut(s) 102, 261
FauI CCCGC 1 cut(s) 208
FblI GTMKAC 1 cut(s) 45
Fnu4HI GCNGC 5 cut(s) 28, 189, 192, 237, 345
FokI GGATG 2 cut(s) 25, 100
Fsp4HI GCNGC 5 cut(s) 28, 189, 192, 237, 345
GlaI GCGC 1 cut(s) 240
GluI GCNGC 5 cut(s) 28, 189, 192, 237, 345
GsuI CTGGAG 1 cut(s) 228
HaeII RGCGCY 1 cut(s) 242
HapII CCGG 4 cut(s) 9, 155, 206, 249
HhaI GCGC 1 cut(s) 241
Hin1I GRCGYC 1 cut(s) 239
Hin1II CATG 2 cut(s) 106, 265
Hin6I GCGC 1 cut(s) 239
HinP1I GCGC 1 cut(s) 239
HincII GTYRAC 1 cut(s) 46
HindII GTYRAC 1 cut(s) 46
HinfI GANTC 1 cut(s) 149
HpaII CCGG 4 cut(s) 9, 155, 206, 249
HphI GGTGA 3 cut(s) 144, 178, 329
Hpy166II GTNNAC 1 cut(s) 46
Hpy188I TCNGA 1 cut(s) 136
Hpy188III TCNNGA 1 cut(s) 301
Hpy8I GTNNAC 1 cut(s) 46
Hpy99I CGWCG 1 cut(s) 163
HpyAV CCTTC 4 cut(s) 88, 139, 307, 325
HpyCH4III ACNGT 2 cut(s) 163, 293
HpyCH4IV ACGT 1 cut(s) 223
HpyF10VI GCNNNNNNNGC 3 cut(s) 185, 200, 245
HpySE526I ACGT 1 cut(s) 223
Hsp92I GRCGYC 1 cut(s) 239
Hsp92II CATG 2 cut(s) 106, 265
HspAI GCGC 1 cut(s) 239
KasI GGCGCC 1 cut(s) 238
KroI GCCGGC 1 cut(s) 248
KroNI GCCGGC 1 cut(s) 250
Kzo9I GATC 1 cut(s) 271
LmnI GCTCC 1 cut(s) 208
Lsp1109I GCAGC 3 cut(s) 200, 203, 331
LweI GCATC 1 cut(s) 361
MabI ACCWGGT 1 cut(s) 170
MaeII ACGT 1 cut(s) 223
MaeIII GTNAC 2 cut(s) 166, 224
MalI GATC 1 cut(s) 273
MbiI CCGCTC 1 cut(s) 203
MboI GATC 1 cut(s) 271
Mly113I GGCGCC 1 cut(s) 239
MmeI TCCRAC 2 cut(s) 89, 348
MnlI CCTC 3 cut(s) 17, 55, 252
MroNI GCCGGC 1 cut(s) 248
MseI TTAA 1 cut(s) 56
MspI CCGG 4 cut(s) 9, 155, 206, 249
MspR9I CCNGG 3 cut(s) 10, 172, 233
MvaI CCWGG 2 cut(s) 172, 233
MwoI GCNNNNNNNGC 3 cut(s) 185, 200, 245
NaeI GCCGGC 1 cut(s) 250
NarI GGCGCC 1 cut(s) 239
NciI CCSGG 1 cut(s) 10
NdeII GATC 1 cut(s) 271
NgoMIV GCCGGC 1 cut(s) 248
NlaIII CATG 2 cut(s) 106, 265
NlaIV GGNNCC 1 cut(s) 240
NmuCI GTSAC 1 cut(s) 166
PcsI WCGNNNNNNNCGW 1 cut(s) 297
PdiI GCCGGC 1 cut(s) 250
PfeI GAWTC 1 cut(s) 149
PflFI GACNNNGTC 2 cut(s) 41, 307
PfoI TCCNGGA 1 cut(s) 8
PkrI GCNGC 5 cut(s) 29, 190, 193, 238, 346
PluTI GGCGCC 1 cut(s) 242
Psp6I CCWGG 2 cut(s) 170, 231
PspEI GGTNACC 1 cut(s) 166
PspGI CCWGG 2 cut(s) 170, 231
PspN4I GGNNCC 1 cut(s) 240
PspPI GGNCC 1 cut(s) 6
PsyI GACNNNGTC 2 cut(s) 41, 307
RsaI GTAC 1 cut(s) 80
RsaNI GTAC 1 cut(s) 79
SalI GTCGAC 1 cut(s) 44
SaqAI TTAA 1 cut(s) 56
SatI GCNGC 5 cut(s) 28, 189, 192, 237, 345
Sau3AI GATC 1 cut(s) 271
Sau96I GGNCC 1 cut(s) 6
ScrFI CCNGG 3 cut(s) 10, 172, 233
SetI ASST 4 cut(s) 8, 176, 196, 226
SexAI ACCWGGT 1 cut(s) 170
SfaNI GCATC 1 cut(s) 361
SfoI GGCGCC 1 cut(s) 240
SgrAI CRCCGGYG 1 cut(s) 154
SinI GGWCC 1 cut(s) 6
SsiI CCGC 4 cut(s) 27, 186, 201, 236
SspDI GGCGCC 1 cut(s) 238
StyD4I CCNGG 3 cut(s) 8, 170, 231
TaaI ACNGT 2 cut(s) 163, 293
TaiI ACGT 1 cut(s) 226
TaqI TCGA 3 cut(s) 45, 300, 311
TatI WGTACW 1 cut(s) 78
TauI GCSGC 2 cut(s) 30, 239
TfiI GAWTC 1 cut(s) 149
Tru1I TTAA 1 cut(s) 56
Tru9I TTAA 1 cut(s) 56
TseFI GTSAC 1 cut(s) 166
TseI GCWGC 3 cut(s) 188, 191, 344
Tsp45I GTSAC 1 cut(s) 166
Tth111I GACNNNGTC 2 cut(s) 41, 307
VpaK11BI GGWCC 1 cut(s) 6
XmiI GTMKAC 1 cut(s) 45
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.