AT3G22240

Cysteine-rich and transmembrane domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0001954
Physical Location & Seq
Reverse (-)
7863606 .. 7864895
1290 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G22240.1

Sequence Viewer

Length: 219 bp
ATGAATCCATCCGAGCAGAATCACTTGTCCGTGGAAAAACCTTCACAGACTTCTTCGGGGCCGTACACAAGTCCACCACCGATTGGTTATCCGACCAGAGATGCGATGGTTGGTGATCCTCCAGCCGCCGCAGTGGAGACAAAGTCCAAGGGCGATGGTTTTTGGAAAGGATGTTGTGCTGCCATATGCTGCTGTTGTGTCCTGGATGCATGCTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CYSTM

Protein Analysis

72

Amino Acids

7.52

Weight (kDa)

5.0

Isoelectric Point (pI)

42.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CYSTM PF12734 29 - 72 1.6e-14 Cysteine-rich TM module stress tolerance
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 83
AciI CCGC 2 cut(s) 126, 129
AclWI GGATC 1 cut(s) 110
AfaI GTAC 1 cut(s) 65
AfiI CCNNNNNNNGG 1 cut(s) 83
AjnI CCWGG 1 cut(s) 201
Alw26I GTCTC 1 cut(s) 131
AlwI GGATC 1 cut(s) 110
AoxI GGCC 1 cut(s) 59
ApeKI GCWGC 2 cut(s) 179, 189
AspS9I GGNCC 1 cut(s) 59
AsuHPI GGTGA 1 cut(s) 125
BbvI GCAGC 2 cut(s) 166, 176
BccI CCATC 3 cut(s) 16, 100, 149
BceAI ACGGC 1 cut(s) 46
BciT130I CCWGG 1 cut(s) 203
BcoDI GTCTC 1 cut(s) 131
BisI GCNGC 4 cut(s) 126, 129, 180, 190
BlsI GCNGC 4 cut(s) 127, 130, 181, 191
Bme1390I CCNGG 1 cut(s) 203
BmgT120I GGNCC 1 cut(s) 59
BmiI GGNNCC 1 cut(s) 60
BmrFI CCNGG 1 cut(s) 203
BmsI GCATC 2 cut(s) 91, 196
BpmI CTGGAG 1 cut(s) 105
BsaJI CCNNGG 2 cut(s) 30, 147
BsaXI ACNNNNNCTCC 2 cut(s) 128, 158
Bsc4I CCNNNNNNNGG 1 cut(s) 83
BseBI CCWGG 1 cut(s) 203
BseDI CCNNGG 2 cut(s) 30, 147
BseGI GGATG 3 cut(s) 8, 176, 211
BseLI CCNNNNNNNGG 1 cut(s) 83
BseXI GCAGC 2 cut(s) 166, 176
BshFI GGCC 1 cut(s) 61
BslI CCNNNNNNNGG 1 cut(s) 83
BsmAI GTCTC 1 cut(s) 131
BsnI GGCC 1 cut(s) 61
Bsp143I GATC 1 cut(s) 115
BspACI CCGC 2 cut(s) 126, 129
BspANI GGCC 1 cut(s) 61
BspLI GGNNCC 1 cut(s) 60
BspPI GGATC 1 cut(s) 110
BssECI CCNNGG 2 cut(s) 30, 147
BssMI GATC 1 cut(s) 115
BssT1I CCWWGG 1 cut(s) 147
Bst2UI CCWGG 1 cut(s) 203
BstC8I GCNNGC 1 cut(s) 211
BstDSI CCRYGG 1 cut(s) 30
BstF5I GGATG 3 cut(s) 8, 176, 211
BstKTI GATC 1 cut(s) 118
BstMAI GTCTC 1 cut(s) 131
BstMBI GATC 1 cut(s) 115
BstNI CCWGG 1 cut(s) 203
BstNSI RCATGY 1 cut(s) 213
BstSCI CCNGG 1 cut(s) 201
BstV1I GCAGC 2 cut(s) 166, 176
BsuRI GGCC 1 cut(s) 61
BtgI CCRYGG 1 cut(s) 30
BtgZI GCGATG 2 cut(s) 119, 168
BtsCI GGATG 3 cut(s) 8, 176, 211
BtsI GCAGTG 1 cut(s) 138
BtsIMutI CAGTG 1 cut(s) 138
Cac8I GCNNGC 1 cut(s) 211
Cfr13I GGNCC 1 cut(s) 59
Csp6I GTAC 1 cut(s) 64
CviAII CATG 1 cut(s) 210
CviJI RGCY 2 cut(s) 61, 125
CviKI_1 RGCY 2 cut(s) 61, 125
CviQI GTAC 1 cut(s) 64
DpnI GATC 1 cut(s) 117
DpnII GATC 1 cut(s) 115
Eco130I CCWWGG 1 cut(s) 147
EcoRII CCWGG 1 cut(s) 201
EcoT14I CCWWGG 1 cut(s) 147
EcoT22I ATGCAT 1 cut(s) 211
ErhI CCWWGG 1 cut(s) 147
FaeI CATG 1 cut(s) 213
FaiI YATR 3 cut(s) 185, 187, 211
FatI CATG 1 cut(s) 209
FauNDI CATATG 1 cut(s) 185
Fnu4HI GCNGC 4 cut(s) 126, 129, 180, 190
FokI GGATG 1 cut(s) 183
Fsp4HI GCNGC 4 cut(s) 126, 129, 180, 190
GluI GCNGC 4 cut(s) 126, 129, 180, 190
GsuI CTGGAG 1 cut(s) 105
HaeIII GGCC 1 cut(s) 61
Hin1II CATG 1 cut(s) 213
HinfI GANTC 2 cut(s) 4, 19
HphI GGTGA 1 cut(s) 125
Hpy166II GTNNAC 2 cut(s) 66, 74
Hpy188I TCNGA 3 cut(s) 13, 93, 218
Hpy8I GTNNAC 2 cut(s) 66, 74
HpyAV CCTTC 1 cut(s) 51
HpyCH4V TGCA 1 cut(s) 209
Hsp92II CATG 1 cut(s) 213
Kzo9I GATC 1 cut(s) 115
LpnPI CCDG 4 cut(s) 109, 135, 188, 215
Lsp1109I GCAGC 2 cut(s) 166, 176
LweI GCATC 2 cut(s) 91, 196
MalI GATC 1 cut(s) 117
MboI GATC 1 cut(s) 115
MboII GAAGA 1 cut(s) 45
MmeI TCCRAC 1 cut(s) 116
MnlI CCTC 1 cut(s) 129
Mph1103I ATGCAT 1 cut(s) 211
MspR9I CCNGG 1 cut(s) 203
MvaI CCWGG 1 cut(s) 203
NdeI CATATG 1 cut(s) 185
NdeII GATC 1 cut(s) 115
NlaIII CATG 1 cut(s) 213
NlaIV GGNNCC 1 cut(s) 60
NsiI ATGCAT 1 cut(s) 211
NspI RCATGY 1 cut(s) 213
PaeI GCATGC 1 cut(s) 213
PfeI GAWTC 2 cut(s) 4, 19
PflFI GACNNNGTC 1 cut(s) 142
PflMI CCANNNNNTGG 1 cut(s) 83
PfoI TCCNGGA 1 cut(s) 201
PkrI GCNGC 4 cut(s) 127, 130, 181, 191
Psp6I CCWGG 1 cut(s) 201
PspGI CCWGG 1 cut(s) 201
PspN4I GGNNCC 1 cut(s) 60
PspPI GGNCC 1 cut(s) 59
PsyI GACNNNGTC 1 cut(s) 142
RsaI GTAC 1 cut(s) 65
RsaNI GTAC 1 cut(s) 64
SatI GCNGC 4 cut(s) 126, 129, 180, 190
Sau3AI GATC 1 cut(s) 115
Sau96I GGNCC 1 cut(s) 59
ScrFI CCNGG 1 cut(s) 203
SetI ASST 1 cut(s) 43
SfaNI GCATC 2 cut(s) 91, 196
SphI GCATGC 1 cut(s) 213
SsiI CCGC 2 cut(s) 126, 129
StyD4I CCNGG 1 cut(s) 201
StyI CCWWGG 1 cut(s) 147
TauI GCSGC 2 cut(s) 128, 131
TfiI GAWTC 2 cut(s) 4, 19
TscAI CASTG 1 cut(s) 138
TseI GCWGC 2 cut(s) 179, 189
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 1 cut(s) 19
TspRI CASTG 1 cut(s) 138
Tth111I GACNNNGTC 1 cut(s) 142
Van91I CCANNNNNTGG 1 cut(s) 83
XceI RCATGY 1 cut(s) 213
XcmI CCANNNNNNNNNTGG 1 cut(s) 103
Zsp2I ATGCAT 1 cut(s) 211
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.