AT3G45290

May be involved in modulation of pathogen defense and leaf cell death

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0002520
Physical Location & Seq
Forward (+)
16617398 .. 16621370
3973 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G45290.1

Sequence Viewer

Length: 1527 bp
ATGACGGATAAAGAAGAAAGCAACCACTCCTCGGAGGTTGGCGCCGTCCGATCCCTCCAAGAAACTCCCACTTGGGCTCTGGCCACCGTTTGTTTCTTCTTCATCGCTGTTTCCATTTGCCTCGAGCGTCTCATCAATCTTCTATCCACTAGACTTAAGAAAAATAGAAAAACGTCGCTACTTGAAGCTGTAGAGAAGCTCAAATCAGTTCTAATGGTGCTAGGATTCATGTCACTAATGCTAAATGTAACTGAAGGAGAAGTTTCGAAGATATGCATTCCTATAAAGTATGCCAATCGAATGTTGCCATGCCGTAAAACCATAAAATCACATAATGATGTTAGTGAAGATGATGATGATGATGATGGTGATAATCATGACAACAGCTTCTTTCATCAATGCTCTTCCAAGGGTAAGACTTCACTAATATCAGAAGAAGGGTTGACTCAGTTGAGTTACTTCTTCTTTGTGTTGGCATGCATGCATATTCTCTGCAATCTTGCTATTCTTCTCCTTGGAATGGCCAAGATGAGAAAATGGAATTCGTGGGAGAAAGAGACCCAAACAGTGGAGTATCTAGCAGCAAACGATCCGAATAGGTTTAGAATAACAAGAGATACAACATTTGCTCGACGACACCTGAGTTCATGGACTGAAACATCATTTCAACTTTGGATTAAATGTTTCTTCAGGCAATTTTACAACTCAGTGGCGAAAGTAGATTACCTCACACTTCGGCATGGGTTCATCTTTGCTCATGTATCGTCAAATAATGCTTTCAACTTCCAAAACTATATACAGAGATCTTTACATGAAGATTTTAAAACTGTGGTTGGTATAAGTCCTTTAATGTGGCTAACTGTGGTCATCTTTATGCTCCTCGACGTTTCTGGTTGGAGAGTATATTTTTATATGTCATTTGTGCCACTCATTATAGTTTTAGTGATCGGGACGAAACTAGAGATGATAGTAGCGAAAATGGCGGTCACAATTAAGGAGAATAACAGTGTAATTAGAGGAACCCCTCTCGTTGAGTCAAACGACACGCATTTCTGGTTTTCCAATCCTCGTTTTCTCTTAAGCATTCTACACTACACGCTGTTTCTGAACACCTTCGAGATGGCATTTATTGTGTGGATCACTTGGCAATTTGGGATTAACTCTTGCTACCATGATAACCAAGGGATCATAATCACACGACTTGTATTAGCGGTGACAGTCCAGTTCTTGAGCAGCTACATTACATTGCCTCTTTATGCCATTGTAACTCAGATGGGGTCAAGCTACAAGAGAGCAATCTTAGAAGAACAACTAGCAAATGTGTTAAGGCACTGGCAAGGGATGGTTAGAGACAAGAAAAAGACTATCCAAACGCCAGACACCGACAACAACAGTAACAATAACAATGGTGATATTGATTCTGGAGAAAGTCCGGTTCAGACAGAAGTTGCTTCTGAGTTTAGATTTTCGGGTAGACAATCGCCGATTTTACAAGAGATACAGATACAAGAGAAAACTGAAAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0006952 GO:0008150 GO:0016020 GO:0044464 GO:0050896 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mlo

Protein Analysis

508

Amino Acids

58.56

Weight (kDa)

7.2

Isoelectric Point (pI)

38.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mlo PF03094 17 - 481 8.1e-180 Mlo family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 41
AccB7I CCANNNNNTGG 1 cut(s) 568
AccI GTMKAC 1 cut(s) 1474
AciI CCGC 2 cut(s) 983, 1211
AclWI GGATC 4 cut(s) 45, 584, 1145, 1193
AcoI YGGCCR 2 cut(s) 81, 522
AcsI RAATTY 1 cut(s) 541
AcuI CTGAAG 2 cut(s) 273, 673
AcyI GRCGYC 1 cut(s) 42
AfiI CCNNNNNNNGG 3 cut(s) 31, 520, 568
AflII CTTAAG 2 cut(s) 155, 1078
AgsI TTSAA 3 cut(s) 185, 668, 781
AluBI AGCT 5 cut(s) 188, 199, 387, 1236, 1284
AluI AGCT 5 cut(s) 188, 199, 387, 1236, 1284
Alw26I GTCTC 3 cut(s) 134, 551, 1344
AlwI GGATC 4 cut(s) 45, 584, 1145, 1193
Ama87I CYCGRG 1 cut(s) 122
AoxI GGCC 2 cut(s) 81, 522
ApeKI GCWGC 2 cut(s) 581, 1233
ApoI RAATTY 1 cut(s) 541
Asp700I GAANNNNTTC 1 cut(s) 1112
AspLEI GCGC 1 cut(s) 44
AsuHPI GGTGA 3 cut(s) 380, 1225, 1421
AsuII TTCGAA 1 cut(s) 266
AvaI CYCGRG 1 cut(s) 122
BalI TGGCCA 2 cut(s) 83, 524
BanI GGYRCC 1 cut(s) 41
BanII GRGCYC 1 cut(s) 79
BbvI GCAGC 2 cut(s) 593, 1245
BccI CCATC 4 cut(s) 359, 1114, 1267, 1336
BceAI ACGGC 2 cut(s) 29, 297
BcoDI GTCTC 3 cut(s) 134, 551, 1344
BfaI CTAG 5 cut(s) 150, 221, 578, 959, 1313
BfmI CTRYAG 1 cut(s) 189
BfoI RGCGCY 1 cut(s) 45
BfrI CTTAAG 2 cut(s) 155, 1078
BglII AGATCT 1 cut(s) 803
BisI GCNGC 2 cut(s) 582, 1234
BlsI GCNGC 2 cut(s) 583, 1235
BmeT110I CYCGRG 1 cut(s) 122
BmiI GGNNCC 2 cut(s) 43, 1021
BpmI CTGGAG 1 cut(s) 1443
Bpu14I TTCGAA 1 cut(s) 266
BpuEI CTTGAG 1 cut(s) 1249
BsaBI GATNNNNATC 1 cut(s) 1190
BsaHI GRCGYC 1 cut(s) 42
BsaI GGTCTC 1 cut(s) 551
BsaJI CCNNGG 4 cut(s) 30, 408, 514, 1180
BsaWI WCCGGW 1 cut(s) 1432
Bsc4I CCNNNNNNNGG 3 cut(s) 31, 520, 568
Bse1I ACTGG 2 cut(s) 1222, 1337
Bse3DI GCAATG 1 cut(s) 1244
Bse8I GATNNNNATC 1 cut(s) 1190
BseDI CCNNGG 4 cut(s) 30, 408, 514, 1180
BseGI GGATG 1 cut(s) 1347
BseJI GATNNNNATC 1 cut(s) 1190
BseLI CCNNNNNNNGG 3 cut(s) 31, 520, 568
BseMI GCAATG 1 cut(s) 1244
BseMII CTCAG 5 cut(s) 461, 632, 720, 1283, 1446
BseNI ACTGG 2 cut(s) 1222, 1337
BseRI GAGGAG 2 cut(s) 19, 869
BseXI GCAGC 2 cut(s) 593, 1245
BshFI GGCC 2 cut(s) 83, 524
BshNI GGYRCC 1 cut(s) 41
BsiHKCI CYCGRG 1 cut(s) 122
BsiSI CCGG 1 cut(s) 1433
BslFI GGGAC 1 cut(s) 964
BslI CCNNNNNNNGG 3 cut(s) 31, 520, 568
BsmAI GTCTC 3 cut(s) 134, 551, 1344
BsmBI CGTCTC 1 cut(s) 134
BsmFI GGGAC 1 cut(s) 964
BsmI GAATGC 2 cut(s) 276, 1083
BsnI GGCC 2 cut(s) 83, 524
Bso31I GGTCTC 1 cut(s) 551
BsoBI CYCGRG 1 cut(s) 122
Bsp119I TTCGAA 1 cut(s) 266
Bsp1286I GDGCHC 1 cut(s) 79
Bsp143I GATC 6 cut(s) 50, 589, 803, 945, 1137, 1185
BspACI CCGC 2 cut(s) 983, 1211
BspANI GGCC 2 cut(s) 83, 524
BspCNI CTCAG 5 cut(s) 460, 633, 719, 1282, 1447
BspHI TCATGA 1 cut(s) 376
BspLI GGNNCC 2 cut(s) 43, 1021
BspPI GGATC 4 cut(s) 45, 584, 1145, 1193
BspQI GCTCTTC 1 cut(s) 409
BspT104I TTCGAA 1 cut(s) 266
BspT107I GGYRCC 1 cut(s) 41
BspTI CTTAAG 2 cut(s) 155, 1078
BspTNI GGTCTC 1 cut(s) 551
BsrDI GCAATG 1 cut(s) 1244
BsrI ACTGG 2 cut(s) 1222, 1337
BssECI CCNNGG 4 cut(s) 30, 408, 514, 1180
BssMI GATC 6 cut(s) 50, 589, 803, 945, 1137, 1185
BssNI GRCGYC 1 cut(s) 42
BssT1I CCWWGG 3 cut(s) 408, 514, 1180
Bst4CI ACNGT 7 cut(s) 88, 568, 829, 862, 1007, 1219, 1394
Bst6I CTCTTC 1 cut(s) 409
BstACI GRCGYC 1 cut(s) 42
BstAFI CTTAAG 2 cut(s) 155, 1078
BstBI TTCGAA 1 cut(s) 266
BstC8I GCNNGC 2 cut(s) 478, 482
BstDEI CTNAG 6 cut(s) 447, 641, 706, 1269, 1300, 1455
BstF5I GGATG 1 cut(s) 1347
BstH2I RGCGCY 1 cut(s) 45
BstHHI GCGC 1 cut(s) 44
BstKTI GATC 6 cut(s) 53, 592, 806, 948, 1140, 1188
BstMAI GTCTC 3 cut(s) 134, 551, 1344
BstMBI GATC 6 cut(s) 50, 589, 803, 945, 1137, 1185
BstMWI GCNNNNNNNGC 1 cut(s) 980
BstNSI RCATGY 2 cut(s) 480, 484
BstSFI CTRYAG 1 cut(s) 189
BstV1I GCAGC 2 cut(s) 593, 1245
BstX2I RGATCY 1 cut(s) 803
BstYI RGATCY 1 cut(s) 803
BsuRI GGCC 2 cut(s) 83, 524
BtgZI GCGATG 1 cut(s) 88
BtsCI GGATG 1 cut(s) 1347
BtsIMutI CAGTG 4 cut(s) 573, 714, 1012, 1330
Cac8I GCNNGC 2 cut(s) 478, 482
CciI TCATGA 1 cut(s) 376
CfoI GCGC 1 cut(s) 44
CseI GACGC 1 cut(s) 116
CviJI RGCY 9 cut(s) 77, 83, 188, 199, 387, 524, 856, 1236, 1284
CviKI_1 RGCY 9 cut(s) 77, 83, 188, 199, 387, 524, 856, 1236, 1284
DdeI CTNAG 6 cut(s) 447, 641, 706, 1269, 1300, 1455
DinI GGCGCC 1 cut(s) 43
DpnI GATC 6 cut(s) 52, 591, 805, 947, 1139, 1187
DpnII GATC 6 cut(s) 50, 589, 803, 945, 1137, 1185
DraI TTTAAA 1 cut(s) 823
EaeI YGGCCR 2 cut(s) 81, 522
Eam1104I CTCTTC 1 cut(s) 409
EarI CTCTTC 1 cut(s) 409
Eco130I CCWWGG 3 cut(s) 408, 514, 1180
Eco24I GRGCYC 1 cut(s) 79
Eco31I GGTCTC 1 cut(s) 551
Eco57I CTGAAG 2 cut(s) 273, 673
Eco88I CYCGRG 1 cut(s) 122
EcoRI GAATTC 1 cut(s) 541
EcoT14I CCWWGG 3 cut(s) 408, 514, 1180
EcoT22I ATGCAT 3 cut(s) 278, 482, 486
EcoT38I GRGCYC 1 cut(s) 79
EgeI GGCGCC 1 cut(s) 43
EheI GGCGCC 1 cut(s) 43
ErhI CCWWGG 3 cut(s) 408, 514, 1180
Esp3I CGTCTC 1 cut(s) 134
FaqI GGGAC 1 cut(s) 964
FblI GTMKAC 1 cut(s) 1474
Fnu4HI GCNGC 2 cut(s) 582, 1234
FokI GGATG 1 cut(s) 1354
FriOI GRGCYC 1 cut(s) 79
Fsp4HI GCNGC 2 cut(s) 582, 1234
FspBI CTAG 5 cut(s) 150, 221, 578, 959, 1313
GlaI GCGC 1 cut(s) 43
GluI GCNGC 2 cut(s) 582, 1234
GsuI CTGGAG 1 cut(s) 1443
HaeII RGCGCY 1 cut(s) 45
HaeIII GGCC 2 cut(s) 83, 524
HapII CCGG 1 cut(s) 1433
HgaI GACGC 1 cut(s) 116
HhaI GCGC 1 cut(s) 44
Hin1I GRCGYC 1 cut(s) 42
Hin6I GCGC 1 cut(s) 42
HinP1I GCGC 1 cut(s) 42
HincII GTYRAC 1 cut(s) 444
HindII GTYRAC 1 cut(s) 444
HinfI GANTC 4 cut(s) 225, 445, 1034, 1418
HpaII CCGG 1 cut(s) 1433
HphI GGTGA 3 cut(s) 380, 1225, 1421
Hpy166II GTNNAC 2 cut(s) 444, 1475
Hpy188I TCNGA 8 cut(s) 34, 50, 433, 594, 1107, 1272, 1440, 1456
Hpy188III TCNNGA 5 cut(s) 377, 949, 1117, 1228, 1422
Hpy8I GTNNAC 2 cut(s) 444, 1475
Hpy99I CGWCG 3 cut(s) 178, 636, 887
HpyAV CCTTC 3 cut(s) 248, 431, 1123
HpyCH4III ACNGT 7 cut(s) 88, 568, 829, 862, 1007, 1219, 1394
HpyCH4IV ACGT 2 cut(s) 173, 885
HpyCH4V TGCA 4 cut(s) 276, 480, 484, 495
HpyF10VI GCNNNNNNNGC 1 cut(s) 980
HpyF3I CTNAG 6 cut(s) 447, 641, 706, 1269, 1300, 1455
HpySE526I ACGT 2 cut(s) 173, 885
Hsp92I GRCGYC 1 cut(s) 42
HspAI GCGC 1 cut(s) 42
KasI GGCGCC 1 cut(s) 41
Kzo9I GATC 6 cut(s) 50, 589, 803, 945, 1137, 1185
LguI GCTCTTC 1 cut(s) 409
LmnI GCTCC 1 cut(s) 882
Lsp1109I GCAGC 2 cut(s) 593, 1245
MaeI CTAG 5 cut(s) 150, 221, 578, 959, 1313
MaeII ACGT 2 cut(s) 173, 885
MaeIII GTNAC 7 cut(s) 231, 247, 455, 985, 1213, 1264, 1394
MalI GATC 6 cut(s) 52, 591, 805, 947, 1139, 1187
MboI GATC 6 cut(s) 50, 589, 803, 945, 1137, 1185
MflI RGATCY 1 cut(s) 803
MhlI GDGCHC 1 cut(s) 79
MlsI TGGCCA 2 cut(s) 83, 524
MluCI AATT 5 cut(s) 541, 695, 990, 1011, 1148
MluNI TGGCCA 2 cut(s) 83, 524
Mly113I GGCGCC 1 cut(s) 42
MlyI GAGTC 2 cut(s) 439, 1043
MmeI TCCRAC 1 cut(s) 875
Mox20I TGGCCA 2 cut(s) 83, 524
Mph1103I ATGCAT 3 cut(s) 278, 482, 486
MroXI GAANNNNTTC 1 cut(s) 1112
MscI TGGCCA 2 cut(s) 83, 524
MseI TTAA 8 cut(s) 156, 678, 822, 848, 993, 1079, 1158, 1325
MslI CAYNNNNRTG 2 cut(s) 336, 872
Msp20I TGGCCA 2 cut(s) 83, 524
MspCI CTTAAG 2 cut(s) 155, 1078
MspI CCGG 1 cut(s) 1433
Mva1269I GAATGC 2 cut(s) 276, 1083
MwoI GCNNNNNNNGC 1 cut(s) 980
NarI GGCGCC 1 cut(s) 42
NdeII GATC 6 cut(s) 50, 589, 803, 945, 1137, 1185
NlaIV GGNNCC 2 cut(s) 43, 1021
NmuCI GTSAC 3 cut(s) 231, 985, 1213
NsiI ATGCAT 3 cut(s) 278, 482, 486
NspI RCATGY 2 cut(s) 480, 484
NspV TTCGAA 1 cut(s) 266
PaeI GCATGC 2 cut(s) 480, 484
PaeR7I CTCGAG 1 cut(s) 122
PagI TCATGA 1 cut(s) 376
PciSI GCTCTTC 1 cut(s) 409
PctI GAATGC 2 cut(s) 276, 1083
PdmI GAANNNNTTC 1 cut(s) 1112
PfeI GAWTC 2 cut(s) 225, 1418
PflMI CCANNNNNTGG 1 cut(s) 568
PkrI GCNGC 2 cut(s) 583, 1235
PleI GAGTC 2 cut(s) 439, 1042
PluTI GGCGCC 1 cut(s) 45
PpsI GAGTC 2 cut(s) 439, 1042
PspN4I GGNNCC 2 cut(s) 43, 1021
PspXI VCTCGAGB 1 cut(s) 122
PsuI RGATCY 1 cut(s) 803
RseI CAYNNNNRTG 2 cut(s) 336, 872
SapI GCTCTTC 1 cut(s) 409
SaqAI TTAA 8 cut(s) 156, 678, 822, 848, 993, 1079, 1158, 1325
SatI GCNGC 2 cut(s) 582, 1234
Sau3AI GATC 6 cut(s) 50, 589, 803, 945, 1137, 1185
SchI GAGTC 2 cut(s) 439, 1043
SduI GDGCHC 1 cut(s) 79
SfcI CTRYAG 1 cut(s) 189
SfoI GGCGCC 1 cut(s) 43
Sfr274I CTCGAG 1 cut(s) 122
SfuI TTCGAA 1 cut(s) 266
SlaI CTCGAG 1 cut(s) 122
SmiMI CAYNNNNRTG 2 cut(s) 336, 872
SmlI CTYRAG 4 cut(s) 122, 155, 1078, 1228
SmoI CTYRAG 4 cut(s) 122, 155, 1078, 1228
SphI GCATGC 2 cut(s) 480, 484
Sse9I AATT 5 cut(s) 541, 695, 990, 1011, 1148
SsiI CCGC 2 cut(s) 983, 1211
SspDI GGCGCC 1 cut(s) 41
SspMI CTAG 5 cut(s) 150, 221, 578, 959, 1313
StyI CCWWGG 3 cut(s) 408, 514, 1180
TaaI ACNGT 7 cut(s) 88, 568, 829, 862, 1007, 1219, 1394
TaiI ACGT 2 cut(s) 176, 888
TaqI TCGA 6 cut(s) 123, 266, 298, 631, 882, 1116
TasI AATT 5 cut(s) 541, 695, 990, 1011, 1148
TfiI GAWTC 2 cut(s) 225, 1418
Tru1I TTAA 8 cut(s) 156, 678, 822, 848, 993, 1079, 1158, 1325
Tru9I TTAA 8 cut(s) 156, 678, 822, 848, 993, 1079, 1158, 1325
TscAI CASTG 4 cut(s) 573, 714, 1012, 1337
TseFI GTSAC 3 cut(s) 231, 985, 1213
TseI GCWGC 2 cut(s) 581, 1233
Tsp45I GTSAC 3 cut(s) 231, 985, 1213
TspDTI ATGAA 6 cut(s) 91, 217, 383, 636, 736, 828
TspGWI ACGGA 1 cut(s) 20
TspRI CASTG 4 cut(s) 573, 714, 1012, 1337
Van91I CCANNNNNTGG 1 cut(s) 568
Vha464I CTTAAG 2 cut(s) 155, 1078
XapI RAATTY 1 cut(s) 541
XceI RCATGY 2 cut(s) 480, 484
XcmI CCANNNNNNNNNTGG 1 cut(s) 76
XhoI CTCGAG 1 cut(s) 122
XmiI GTMKAC 1 cut(s) 1474
XmnI GAANNNNTTC 1 cut(s) 1112
XspI CTAG 5 cut(s) 150, 221, 578, 959, 1313
Zsp2I ATGCAT 3 cut(s) 278, 482, 486
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.