FvH4_3g32830

MLO-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0002520
Physical Location & Seq
Forward (+)
28194894 .. 28203155
8262 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g32830.t1

Sequence Viewer

Length: 1659 bp
ATGGCTTCCGAAGGAACAAGTCGCTCTCTGCAAGACACACCCACTTGGGCTCTGGCAACAGTTTGCTTCGTCTTCATCTTTCTCTCTATTTTCATAGAGCACTTGATCCATCTCCTTTCCCAATTTCTCAAACGACATAGAAAGACTGCATTGTTCGAGGCCGTAGAGAAGCTCAAATCAGTGCTTATGCTTCTGGGTTTCATGTCACTAATACTGACAGTAACACAGAGATGGATATCCAAAATTTGCATACCCACGAAAGCTGCAGAAACTATGCTTCCCTGCAGAAAATCAGCATCAACTAAAACTACCAAAGCACTTGGTTTCGATCGCATGTGGAGCACCGGCGCATCTACAGCTTTCGAAGTGATAAACAGAAGGCTAGCGGAAACAGAAGATACTACTAGCACCAGTTCTACCACTTCTTCTGAGGATCAGTGCGAATCCCATGGGAAGACCTCTTTTATATCCCAAGGAGGACTAAATCAGCTCAACAACTTCATCTTTGTTCTGGCAATTATGCAGATTGTGTATAGTGTTCTTACAATGGCTTTAGGAAGAGCCAAGATGAAGCGTTGGGAAGCATGGGAAAGAGAAACGCAGACGGTAGAATACCAAGTTGCAAATGATCCTAATCGATTCAGGTTTACAAGGCAAACAACATTTGCTCGTAGACACATGAACTCTTGTACAGGAACACCTATTCAACTGTGGATTAAATGCTTCTTCAGACAATTCTTCCATTCAGTTGAAAGAGTTGATTATCTCACATTACGCCACGGCTTCATTTCTACTCATTTGTCAACAAACAACGCATTCAATTTCCAGAAGTACATAGAACGGTCGCTGGAGGATGATTTCAGAGAAGTAGTTGGCATAAGCCCCTTGATGTGGTTTATCGTCGTAATCTTCATTCTCGTGGACGTGCATGGTTGGCAGGTGTATCTCTGGGTATCATTTGTGCCACTAATTATTGTGCTGGTTCTCGGAACAAAACTGGAAGTGGTGGTAGCCAGAATGGCTCACCAACTCGGTGACCAGCATAATGTGATCAAAGGAACTCCTCTGGTTCAGCCAAAGGACAGTCACTTCTGGTTCAATAAACCGCGATTCGTCTTGACTCTCGTACATCTCACTCTGTTCATGAATGCGTTTGAACTTGCTTTCTTCATTTGGGTCACGTTGCAGTTTGGGATACACTCTTGCTACCATGAACACATTGAGATTATCATTATAAGAGTGGTCTTAGCGGTTGTCGTTCAAGTTATGTGCAGTTACATTACTCTTCCCCTCTATGCTCTAGTGACCCAGATGGGGTCAAATTTCAAGAGTACCCAAGTGCTTGAAGAGCAAACAACAAAAGTTATGAAGCAGTGGCATGCCGACGTGAAGAAAAAAAGGAAAAAGAATAGGCAAGACAATTCAGGCCACGATTATTCCTCTACTATAGGAAGCAGCAGTAGGAGAACCATGAATTCCCCAGATTTCTCTTCCCATCGCCGGCAACCAACTTTTGCTGCAATGTCCCAGACGGAGATTGTCGAAGATGGTCAAGAAATTGTTGAAGAACATCAAGCAGCAAAGGGTGGCCAAACTGAAATTGAACTAGCTAGTGAGGTACACATAGAAATTTCGGAGGTGAACAGAAGCCAAACGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0006952 GO:0008150 GO:0016020 GO:0044464 GO:0050896 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mlo

Protein Analysis

553

Amino Acids

63.4

Weight (kDa)

8.98

Isoelectric Point (pI)

44.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mlo PF03094 8 - 487 3e-199 Mlo family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1235
AarI CACCTGC 1 cut(s) 928
Acc36I ACCTGC 1 cut(s) 928
AccI GTMKAC 1 cut(s) 673
AccII CGCG 1 cut(s) 1108
AciI CCGC 3 cut(s) 386, 1106, 1250
AclWI GGATC 3 cut(s) 100, 441, 623
AcoI YGGCCR 1 cut(s) 1588
AcsI RAATTY 4 cut(s) 243, 1321, 1474, 1629
AcuI CTGAAG 1 cut(s) 712
AfaI GTAC 5 cut(s) 691, 833, 1128, 1333, 1620
AfiI CCNNNNNNNGG 3 cut(s) 891, 1314, 1500
AjiI CACGTC 2 cut(s) 925, 1387
AluBI AGCT 5 cut(s) 172, 263, 359, 490, 1610
AluI AGCT 5 cut(s) 172, 263, 359, 490, 1610
Alw21I GWGCWC 2 cut(s) 102, 344
AlwI GGATC 3 cut(s) 100, 441, 623
AoxI GGCC 3 cut(s) 159, 1426, 1588
ApeKI GCWGC 4 cut(s) 263, 1455, 1517, 1577
ApoI RAATTY 4 cut(s) 243, 1321, 1474, 1629
AspLEI GCGC 1 cut(s) 350
AsuHPI GGTGA 3 cut(s) 1016, 1046, 1651
AsuII TTCGAA 1 cut(s) 363
AsuNHI GCTAGC 1 cut(s) 382
BalI TGGCCA 1 cut(s) 1590
BanII GRGCYC 1 cut(s) 52
BauI CACGAG 1 cut(s) 917
BbsI GAAGAC 2 cut(s) 64, 461
Bbv12I GWGCWC 2 cut(s) 102, 344
BbvI GCAGC 4 cut(s) 250, 1467, 1504, 1589
BccI CCATC 5 cut(s) 117, 225, 1306, 1503, 1541
BceAI ACGGC 2 cut(s) 146, 796
BciVI GTATCC 1 cut(s) 1188
BclI TGATCA 1 cut(s) 1050
BfaI CTAG 5 cut(s) 383, 405, 1301, 1607, 1611
BfmI CTRYAG 4 cut(s) 264, 283, 354, 1446
BfuAI ACCTGC 1 cut(s) 928
BfuI GTATCC 1 cut(s) 1188
BglI GCCNNNNNGGC 1 cut(s) 1019
BisI GCNGC 4 cut(s) 264, 1456, 1518, 1578
BlsI GCNGC 4 cut(s) 265, 1457, 1519, 1579
BmgBI CACGTC 2 cut(s) 925, 1387
BmsI GCATC 2 cut(s) 305, 359
BmtI GCTAGC 1 cut(s) 386
BpiI GAAGAC 2 cut(s) 64, 461
BpmI CTGGAG 1 cut(s) 869
Bpu14I TTCGAA 1 cut(s) 363
Bsa29I ATCGAT 1 cut(s) 637
BsaBI GATNNNNATC 2 cut(s) 235, 633
BsaJI CCNNGG 3 cut(s) 448, 472, 778
Bsc4I CCNNNNNNNGG 3 cut(s) 891, 1314, 1500
Bse118I RCCGGY 2 cut(s) 344, 1500
Bse1I ACTGG 2 cut(s) 411, 1002
Bse3DI GCAATG 1 cut(s) 1527
Bse8I GATNNNNATC 2 cut(s) 235, 633
BseCI ATCGAT 1 cut(s) 637
BseDI CCNNGG 3 cut(s) 448, 472, 778
BseGI GGATG 1 cut(s) 859
BseJI GATNNNNATC 2 cut(s) 235, 633
BseLI CCNNNNNNNGG 3 cut(s) 891, 1314, 1500
BseMI GCAATG 1 cut(s) 1527
BseMII CTCAG 1 cut(s) 420
BseNI ACTGG 2 cut(s) 411, 1002
BseRI GAGGAG 1 cut(s) 1053
BseXI GCAGC 4 cut(s) 250, 1467, 1504, 1589
BsgI GTGCAG 1 cut(s) 1291
Bsh1236I CGCG 1 cut(s) 1108
Bsh1285I CGRYCG 2 cut(s) 331, 845
BshFI GGCC 3 cut(s) 161, 1428, 1590
BshVI ATCGAT 1 cut(s) 637
BsiEI CGRYCG 2 cut(s) 331, 845
BsiHKAI GWGCWC 2 cut(s) 102, 344
BsiSI CCGG 2 cut(s) 345, 1501
BslFI GGGAC 1 cut(s) 1510
BslI CCNNNNNNNGG 3 cut(s) 891, 1314, 1500
BsmFI GGGAC 1 cut(s) 1510
BsmI GAATGC 2 cut(s) 815, 1153
BsnI GGCC 3 cut(s) 161, 1428, 1590
Bsp119I TTCGAA 1 cut(s) 363
Bsp1286I GDGCHC 3 cut(s) 52, 102, 344
Bsp1407I TGTACA 1 cut(s) 689
Bsp143I GATC 5 cut(s) 105, 328, 433, 628, 1050
Bsp19I CCATGG 1 cut(s) 448
BspACI CCGC 3 cut(s) 386, 1106, 1250
BspANI GGCC 3 cut(s) 161, 1428, 1590
BspCNI CTCAG 1 cut(s) 421
BspDI ATCGAT 1 cut(s) 637
BspFNI CGCG 1 cut(s) 1108
BspHI TCATGA 1 cut(s) 1143
BspMAI CTGCAG 2 cut(s) 268, 287
BspMI ACCTGC 1 cut(s) 928
BspOI GCTAGC 1 cut(s) 386
BspPI GGATC 3 cut(s) 100, 441, 623
BspQI GCTCTTC 2 cut(s) 553, 1341
BspT104I TTCGAA 1 cut(s) 363
BsrDI GCAATG 1 cut(s) 1527
BsrFI RCCGGY 2 cut(s) 344, 1500
BsrGI TGTACA 1 cut(s) 689
BsrI ACTGG 2 cut(s) 411, 1002
BssAI RCCGGY 2 cut(s) 344, 1500
BssECI CCNNGG 3 cut(s) 448, 472, 778
BssMI GATC 5 cut(s) 105, 328, 433, 628, 1050
BssSI CACGAG 1 cut(s) 917
BssT1I CCWWGG 2 cut(s) 448, 472
Bst2BI CACGAG 1 cut(s) 917
Bst4CI ACNGT 6 cut(s) 61, 220, 607, 711, 843, 1085
Bst6I CTCTTC 4 cut(s) 553, 1290, 1341, 1495
BstAUI TGTACA 1 cut(s) 689
BstBI TTCGAA 1 cut(s) 363
BstC8I GCNNGC 3 cut(s) 384, 1380, 1502
BstDEI CTNAG 2 cut(s) 429, 1246
BstDSI CCRYGG 2 cut(s) 448, 778
BstEII GGTNACC 1 cut(s) 1034
BstF5I GGATG 1 cut(s) 859
BstFNI CGCG 1 cut(s) 1108
BstHHI GCGC 1 cut(s) 350
BstKTI GATC 5 cut(s) 108, 331, 436, 631, 1053
BstMBI GATC 5 cut(s) 105, 328, 433, 628, 1050
BstMCI CGRYCG 2 cut(s) 331, 845
BstMWI GCNNNNNNNGC 5 cut(s) 339, 356, 934, 1019, 1348
BstNSI RCATGY 2 cut(s) 337, 1382
BstPI GGTNACC 1 cut(s) 1034
BstSFI CTRYAG 4 cut(s) 264, 283, 354, 1446
BstUI CGCG 1 cut(s) 1108
BstV1I GCAGC 4 cut(s) 250, 1467, 1504, 1589
BstV2I GAAGAC 2 cut(s) 64, 461
Bsu15I ATCGAT 1 cut(s) 637
BsuI GTATCC 1 cut(s) 1188
BsuRI GGCC 3 cut(s) 161, 1428, 1590
BsuTUI ATCGAT 1 cut(s) 637
BtgI CCRYGG 2 cut(s) 448, 778
BtgZI GCGATG 1 cut(s) 1481
BtrI CACGTC 2 cut(s) 925, 1387
BtsCI GGATG 1 cut(s) 859
BtsI GCAGTG 1 cut(s) 1379
BtsIMutI CAGTG 3 cut(s) 186, 443, 1379
BveI ACCTGC 1 cut(s) 928
Cac8I GCNNGC 3 cut(s) 384, 1380, 1502
CciI TCATGA 1 cut(s) 1143
CfoI GCGC 1 cut(s) 350
Cfr10I RCCGGY 2 cut(s) 344, 1500
ClaI ATCGAT 1 cut(s) 637
Csp6I GTAC 5 cut(s) 690, 832, 1127, 1332, 1619
CviQI GTAC 5 cut(s) 690, 832, 1127, 1332, 1619
DdeI CTNAG 2 cut(s) 429, 1246
DpnI GATC 5 cut(s) 107, 330, 435, 630, 1052
DpnII GATC 5 cut(s) 105, 328, 433, 628, 1050
EaeI YGGCCR 1 cut(s) 1588
Eam1104I CTCTTC 4 cut(s) 553, 1290, 1341, 1495
EarI CTCTTC 4 cut(s) 553, 1290, 1341, 1495
Eco130I CCWWGG 2 cut(s) 448, 472
Eco24I GRGCYC 1 cut(s) 52
Eco32I GATATC 1 cut(s) 237
Eco57I CTGAAG 1 cut(s) 712
Eco91I GGTNACC 1 cut(s) 1034
EcoO65I GGTNACC 1 cut(s) 1034
EcoRI GAATTC 1 cut(s) 1474
EcoRV GATATC 1 cut(s) 237
EcoT14I CCWWGG 2 cut(s) 448, 472
EcoT38I GRGCYC 1 cut(s) 52
ErhI CCWWGG 2 cut(s) 448, 472
FaqI GGGAC 1 cut(s) 1510
FbaI TGATCA 1 cut(s) 1050
FblI GTMKAC 1 cut(s) 673
Fnu4HI GCNGC 4 cut(s) 264, 1456, 1518, 1578
FokI GGATG 1 cut(s) 866
FriOI GRGCYC 1 cut(s) 52
Fsp4HI GCNGC 4 cut(s) 264, 1456, 1518, 1578
FspBI CTAG 5 cut(s) 383, 405, 1301, 1607, 1611
GlaI GCGC 1 cut(s) 349
GluI GCNGC 4 cut(s) 264, 1456, 1518, 1578
GsuI CTGGAG 1 cut(s) 869
HaeIII GGCC 3 cut(s) 161, 1428, 1590
HapII CCGG 2 cut(s) 345, 1501
HhaI GCGC 1 cut(s) 350
Hin6I GCGC 1 cut(s) 348
HinP1I GCGC 1 cut(s) 348
HincII GTYRAC 1 cut(s) 804
HindII GTYRAC 1 cut(s) 804
HinfI GANTC 4 cut(s) 443, 639, 1110, 1120
HpaII CCGG 2 cut(s) 345, 1501
HphI GGTGA 3 cut(s) 1016, 1046, 1651
Hpy166II GTNNAC 6 cut(s) 648, 674, 804, 922, 1621, 1642
Hpy188I TCNGA 6 cut(s) 10, 430, 731, 863, 989, 1636
Hpy188III TCNNGA 5 cut(s) 826, 1117, 1144, 1327, 1553
Hpy8I GTNNAC 6 cut(s) 648, 674, 804, 922, 1621, 1642
Hpy99I CGWCG 2 cut(s) 905, 1388
HpyAV CCTTC 2 cut(s) 5, 372
HpyCH4III ACNGT 6 cut(s) 61, 220, 607, 711, 843, 1085
HpyCH4IV ACGT 4 cut(s) 924, 1181, 1386, 1655
HpyF10VI GCNNNNNNNGC 5 cut(s) 339, 356, 934, 1019, 1348
HpyF3I CTNAG 2 cut(s) 429, 1246
HpySE526I ACGT 4 cut(s) 924, 1181, 1386, 1655
HspAI GCGC 1 cut(s) 348
KroI GCCGGC 1 cut(s) 1500
KroNI GCCGGC 1 cut(s) 1502
Ksp22I TGATCA 1 cut(s) 1050
Kzo9I GATC 5 cut(s) 105, 328, 433, 628, 1050
LguI GCTCTTC 2 cut(s) 553, 1341
LmnI GCTCC 1 cut(s) 339
Lsp1109I GCAGC 4 cut(s) 250, 1467, 1504, 1589
LweI GCATC 2 cut(s) 305, 359
MaeI CTAG 5 cut(s) 383, 405, 1301, 1607, 1611
MaeII ACGT 4 cut(s) 924, 1181, 1386, 1655
MaeIII GTNAC 7 cut(s) 204, 220, 1034, 1085, 1177, 1274, 1303
MalI GATC 5 cut(s) 107, 330, 435, 630, 1052
MboI GATC 5 cut(s) 105, 328, 433, 628, 1050
MhlI GDGCHC 3 cut(s) 52, 102, 344
MlsI TGGCCA 1 cut(s) 1590
MluNI TGGCCA 1 cut(s) 1590
MlyI GAGTC 1 cut(s) 1114
Mox20I TGGCCA 1 cut(s) 1590
MroNI GCCGGC 1 cut(s) 1500
MscI TGGCCA 1 cut(s) 1590
MseI TTAA 1 cut(s) 717
MslI CAYNNNNRTG 2 cut(s) 229, 917
Msp20I TGGCCA 1 cut(s) 1590
MspI CCGG 2 cut(s) 345, 1501
Mva1269I GAATGC 2 cut(s) 815, 1153
MvnI CGCG 1 cut(s) 1108
MwoI GCNNNNNNNGC 5 cut(s) 339, 356, 934, 1019, 1348
NaeI GCCGGC 1 cut(s) 1502
NcoI CCATGG 1 cut(s) 448
NdeII GATC 5 cut(s) 105, 328, 433, 628, 1050
NgoMIV GCCGGC 1 cut(s) 1500
NheI GCTAGC 1 cut(s) 382
NmuCI GTSAC 5 cut(s) 204, 1034, 1085, 1177, 1303
NspI RCATGY 2 cut(s) 337, 1382
NspV TTCGAA 1 cut(s) 363
PaeI GCATGC 1 cut(s) 1382
PagI TCATGA 1 cut(s) 1143
PaqCI CACCTGC 1 cut(s) 928
PciSI GCTCTTC 2 cut(s) 553, 1341
PctI GAATGC 2 cut(s) 815, 1153
PdiI GCCGGC 1 cut(s) 1502
PfeI GAWTC 3 cut(s) 443, 639, 1110
PkrI GCNGC 4 cut(s) 265, 1457, 1519, 1579
Ple19I CGATCG 1 cut(s) 331
PleI GAGTC 1 cut(s) 1114
PpsI GAGTC 1 cut(s) 1114
PsiI TTATAA 1 cut(s) 1235
PspEI GGTNACC 1 cut(s) 1034
PstI CTGCAG 2 cut(s) 268, 287
PvuI CGATCG 1 cut(s) 331
RsaI GTAC 5 cut(s) 691, 833, 1128, 1333, 1620
RsaNI GTAC 5 cut(s) 690, 832, 1127, 1332, 1619
RseI CAYNNNNRTG 2 cut(s) 229, 917
SapI GCTCTTC 2 cut(s) 553, 1341
SaqAI TTAA 1 cut(s) 717
SatI GCNGC 4 cut(s) 264, 1456, 1518, 1578
Sau3AI GATC 5 cut(s) 105, 328, 433, 628, 1050
SchI GAGTC 1 cut(s) 1114
SduI GDGCHC 3 cut(s) 52, 102, 344
SfaNI GCATC 2 cut(s) 305, 359
SfcI CTRYAG 4 cut(s) 264, 283, 354, 1446
SfuI TTCGAA 1 cut(s) 363
SgrAI CRCCGGYG 1 cut(s) 344
SmiMI CAYNNNNRTG 2 cut(s) 229, 917
SphI GCATGC 1 cut(s) 1382
SsiI CCGC 3 cut(s) 386, 1106, 1250
SspMI CTAG 5 cut(s) 383, 405, 1301, 1607, 1611
StyI CCWWGG 2 cut(s) 448, 472
TaaI ACNGT 6 cut(s) 61, 220, 607, 711, 843, 1085
TaiI ACGT 4 cut(s) 927, 1184, 1389, 1658
TaqI TCGA 5 cut(s) 156, 327, 363, 637, 1542
TatI WGTACW 2 cut(s) 689, 831
TfiI GAWTC 3 cut(s) 443, 639, 1110
Tru1I TTAA 1 cut(s) 717
Tru9I TTAA 1 cut(s) 717
TscAI CASTG 3 cut(s) 186, 443, 1379
TseFI GTSAC 5 cut(s) 204, 1034, 1085, 1177, 1303
TseI GCWGC 4 cut(s) 263, 1455, 1517, 1577
Tsp45I GTSAC 5 cut(s) 204, 1034, 1085, 1177, 1303
TspGWI ACGGA 1 cut(s) 1547
TspRI CASTG 3 cut(s) 186, 443, 1379
XapI RAATTY 4 cut(s) 243, 1321, 1474, 1629
XceI RCATGY 2 cut(s) 337, 1382
XcmI CCANNNNNNNNNTGG 1 cut(s) 49
XmiI GTMKAC 1 cut(s) 673
XspI CTAG 5 cut(s) 383, 405, 1301, 1607, 1611
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.