MD11G1132500.v1.1

MLO-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0002520
Physical Location & Seq
Reverse (-)
12067866 .. 12074113
6248 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1132500.v1.1.491

Sequence Viewer

Length: 1629 bp
ATGGCTTCTGCAGGATCAGCATCCAGCCGTTCACTTCAAGACACCCCCACCTGGGCTTTGGCAGCAGTTTGCTTTGTGTTCATTTTTCTCTCTCTATTTATAGAGCACTTGATCCATCTCATTAGCAAGTGGCTCAAAAAACACAGAAAAACCGCCTTGTTCGAGGCCGTAGAGAAGCTCAAATCAGTGCTTATACTCCTGGGATTTATGTCACTAATTTTAACAGTATCACAAAGATCCATATCCAAAATATGCATACCCACAAAAGTTGCATTCACCATGCTTCCCTGCAGAAAAAATGCACCAACAAAAACCACCAAAGCTCTTGGTTTAGAGCAAATACCAATAGAAAGAATGCTTGCAGCAGCCAATACCAGTGAGGATCACTGTGAATCCCAGGGAAAGACCTCATTTATATCTCAAGGGGGGCTCAATCAGCTCAACAATTTCATCTTTATTTTGGCAGTTATGCAGATTGTGTATAGTGTTCTCACCATGGCTTTAGGAAGGGCCAAGATGAGGCGTTGGGAAGCATGGGAAAAAGAAACTCAGACAGTGGAATACCAAGTGGCAAATGATCCTAATCGGTTTAGGTTTACAAGGCAGACTACATTTGCTCTTCGACATATGAGTTCTTGTACAGGAAGAGGAACCACTGCTCAACTGTGGATTAGGTGCTTCTTCCGGCAGTTCTATCATTCAGTGGACAAAGTTGACTACCTCACTTTGCGCCACGGTTTCGTTTCAACTCATTTGTCCACAAACCACAATGCATTCGATTTCCAAAAGTACATACAACGATCACTGGACGATGATTTTAGAACTGTAGTCGGAATCAGCCCTCTCCTGTGGTTTATTGTCGTCATCTTCATCCTCCTTGATGTCCATGGTTGGCAGGTGTATCTCTTGGTATCATTTGTGCCACTAATTATAGTGCTAGTTCTTGGAACCAAACTTGAAGTAGTAGTAGCAAACATGGCTCATCAACTTCATGACCAGGATAATGTGATTAAAGGAACTCCTCTGGTGCAACCAAAAGACAGTCACTTCTGGTTCAGTCAACCGCAGTTCGTCTTGACTCTTTTACATCTCACTTTGTTTATGAATGCCTTTGAACTTGCTTTCTTTATATGGGTCACGATGCAATTTGGGATACACTCCTGCTACCATGAACACATATATATTATAATCGTACGAGTGGTTTTAGCGGTGACGGTTCAAGTGATGTGCAGTTACATTACTCTTCCCCTCTATGCTCTAGTGACCCAGATGGGATCAAATTTCAAGAGTGCAGCAGTGCTTGAAGAGCAAACAACAAATGTTATAAAGCAGTGGCACGCTGACGTGAAGAGAAGGAGGAAAAATAAATCACACTATTCACAATCTGGTCATGACGAAAATTCGTCTAGAATCATGCATTCCCCTGATTTCTCTTCACATCGCCCCCAATTAACTTTCGCCGAAATGAACCAGAGTCATCCTAGAGAAACTGAGAATTTTCATGATAACCAAGAAATTGTTGAGGATCATCAAGAAGGGGCTGGCCAAAATGAAATTGAATTAGCCAGTAATGCGTCATCTACTGAGGTACATATAGGAATGCCTGAGGTCACCAGAACACACACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0006952 GO:0008150 GO:0016020 GO:0044464 GO:0050896 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mlo

Protein Analysis

543

Amino Acids

62.03

Weight (kDa)

8.66

Isoelectric Point (pI)

43.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mlo PF03094 10 - 469 6.7e-190 Mlo family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1187, 1325
AarI CACCTGC 1 cut(s) 886
Acc36I ACCTGC 1 cut(s) 886
AciI CCGC 3 cut(s) 153, 1064, 1208
AclWI GGATC 7 cut(s) 22, 106, 231, 390, 572, 1282, 1533
AcoI YGGCCR 1 cut(s) 1543
AcsI RAATTY 3 cut(s) 1279, 1399, 1495
AfaI GTAC 4 cut(s) 640, 791, 1194, 1590
AfiI CCNNNNNNNGG 3 cut(s) 51, 52, 519
AgsI TTSAA 8 cut(s) 38, 747, 959, 1115, 1220, 1285, 1304, 1559
AjiI CACGTC 1 cut(s) 1345
AjnI CCWGG 4 cut(s) 50, 198, 396, 996
AluBI AGCT 3 cut(s) 178, 323, 439
AluI AGCT 3 cut(s) 178, 323, 439
Alw21I GWGCWC 1 cut(s) 108
AlwI GGATC 7 cut(s) 22, 106, 231, 390, 572, 1282, 1533
AoxI GGCC 3 cut(s) 165, 510, 1543
ApeKI GCWGC 4 cut(s) 62, 362, 365, 1292
ApoI RAATTY 3 cut(s) 1279, 1399, 1495
AspLEI GCGC 1 cut(s) 732
AspS9I GGNCC 1 cut(s) 510
AsuHPI GGTGA 4 cut(s) 268, 484, 1222, 1603
AxyI CCTNAGG 1 cut(s) 1605
BalI TGGCCA 1 cut(s) 1545
BanII GRGCYC 1 cut(s) 432
Bbv12I GWGCWC 1 cut(s) 108
BbvI GCAGC 4 cut(s) 74, 374, 377, 1304
BccI CCATC 2 cut(s) 123, 1264
BceAI ACGGC 2 cut(s) 12, 152
BciT130I CCWGG 4 cut(s) 52, 200, 398, 998
BciVI GTATCC 1 cut(s) 1146
BfaI CTAG 4 cut(s) 938, 1259, 1407, 1482
BfmI CTRYAG 3 cut(s) 9, 289, 825
BfuAI ACCTGC 1 cut(s) 886
BfuI GTATCC 1 cut(s) 1146
BisI GCNGC 4 cut(s) 63, 363, 366, 1293
BlsI GCNGC 4 cut(s) 64, 364, 367, 1294
Bme1390I CCNGG 4 cut(s) 52, 200, 398, 998
BmgBI CACGTC 1 cut(s) 1345
BmgT120I GGNCC 1 cut(s) 510
BmiI GGNNCC 2 cut(s) 652, 949
BmrFI CCNGG 4 cut(s) 52, 200, 398, 998
BmsI GCATC 2 cut(s) 29, 1131
BpuEI CTTGAG 1 cut(s) 405
BsaBI GATNNNNATC 3 cut(s) 19, 241, 582
BsaJI CCNNGG 7 cut(s) 51, 199, 396, 397, 495, 733, 886
Bsc4I CCNNNNNNNGG 3 cut(s) 51, 52, 519
Bse1I ACTGG 3 cut(s) 375, 810, 1566
Bse21I CCTNAGG 1 cut(s) 1605
Bse8I GATNNNNATC 3 cut(s) 19, 241, 582
BseBI CCWGG 4 cut(s) 52, 200, 398, 998
BseDI CCNNGG 7 cut(s) 51, 199, 396, 397, 495, 733, 886
BseGI GGATG 3 cut(s) 20, 870, 1477
BseJI GATNNNNATC 3 cut(s) 19, 241, 582
BseLI CCNNNNNNNGG 3 cut(s) 51, 52, 519
BseMII CTCAG 4 cut(s) 563, 1482, 1575, 1596
BseNI ACTGG 3 cut(s) 375, 810, 1566
BseRI GAGGAG 1 cut(s) 1011
BseXI GCAGC 4 cut(s) 74, 374, 377, 1304
BsgI GTGCAG 2 cut(s) 1249, 1311
BshFI GGCC 3 cut(s) 167, 512, 1545
BsiHKAI GWGCWC 1 cut(s) 108
BsiSI CCGG 1 cut(s) 685
BsiWI CGTACG 1 cut(s) 1192
BslI CCNNNNNNNGG 3 cut(s) 51, 52, 519
BsmI GAATGC 6 cut(s) 272, 360, 773, 1111, 1417, 1605
BsnI GGCC 3 cut(s) 167, 512, 1545
Bsp1286I GDGCHC 2 cut(s) 108, 432
Bsp1407I TGTACA 1 cut(s) 638
Bsp143I GATC 8 cut(s) 14, 111, 236, 382, 577, 800, 1274, 1525
Bsp19I CCATGG 2 cut(s) 495, 886
BspACI CCGC 3 cut(s) 153, 1064, 1208
BspANI GGCC 3 cut(s) 167, 512, 1545
BspCNI CTCAG 4 cut(s) 562, 1483, 1576, 1597
BspHI TCATGA 3 cut(s) 991, 1390, 1501
BspLI GGNNCC 2 cut(s) 652, 949
BspMAI CTGCAG 2 cut(s) 13, 293
BspMI ACCTGC 1 cut(s) 886
BspPI GGATC 7 cut(s) 22, 106, 231, 390, 572, 1282, 1533
BspQI GCTCTTC 2 cut(s) 624, 1299
BsrGI TGTACA 1 cut(s) 638
BsrI ACTGG 3 cut(s) 375, 810, 1566
BssECI CCNNGG 7 cut(s) 51, 199, 396, 397, 495, 733, 886
BssMI GATC 8 cut(s) 14, 111, 236, 382, 577, 800, 1274, 1525
BssT1I CCWWGG 2 cut(s) 495, 886
Bst2UI CCWGG 4 cut(s) 52, 200, 398, 998
Bst4CI ACNGT 8 cut(s) 226, 389, 556, 666, 737, 826, 1043, 1216
Bst6I CTCTTC 6 cut(s) 624, 640, 1248, 1299, 1343, 1438
BstAUI TGTACA 1 cut(s) 638
BstC8I GCNNGC 3 cut(s) 360, 1338, 1543
BstDEI CTNAG 4 cut(s) 549, 1491, 1584, 1605
BstDSI CCRYGG 3 cut(s) 495, 733, 886
BstEII GGTNACC 1 cut(s) 1609
BstF5I GGATG 3 cut(s) 20, 870, 1477
BstHHI GCGC 1 cut(s) 732
BstKTI GATC 8 cut(s) 17, 114, 239, 385, 580, 803, 1277, 1528
BstMBI GATC 8 cut(s) 14, 111, 236, 382, 577, 800, 1274, 1525
BstMWI GCNNNNNNNGC 6 cut(s) 17, 62, 436, 977, 1306, 1571
BstNI CCWGG 4 cut(s) 52, 200, 398, 998
BstPI GGTNACC 1 cut(s) 1609
BstSCI CCNGG 4 cut(s) 50, 198, 396, 996
BstSFI CTRYAG 3 cut(s) 9, 289, 825
BstV1I GCAGC 4 cut(s) 74, 374, 377, 1304
BstX2I RGATCY 1 cut(s) 236
BstYI RGATCY 1 cut(s) 236
Bsu36I CCTNAGG 1 cut(s) 1605
BsuI GTATCC 1 cut(s) 1146
BsuRI GGCC 3 cut(s) 167, 512, 1545
BtgI CCRYGG 3 cut(s) 495, 733, 886
BtgZI GCGATG 1 cut(s) 1424
BtrI CACGTC 1 cut(s) 1345
BtsCI GGATG 3 cut(s) 20, 870, 1477
BtsI GCAGTG 3 cut(s) 654, 1302, 1337
BtsIMutI CAGTG 9 cut(s) 192, 382, 385, 561, 654, 708, 803, 1302, 1337
BveI ACCTGC 1 cut(s) 886
Cac8I GCNNGC 3 cut(s) 360, 1338, 1543
CciI TCATGA 3 cut(s) 991, 1390, 1501
CfoI GCGC 1 cut(s) 732
Cfr13I GGNCC 1 cut(s) 510
CseI GACGC 1 cut(s) 1563
Csp6I GTAC 4 cut(s) 639, 790, 1193, 1589
CspCI CAANNNNNGTGG 2 cut(s) 250, 285
CviQI GTAC 4 cut(s) 639, 790, 1193, 1589
DdeI CTNAG 4 cut(s) 549, 1491, 1584, 1605
DpnI GATC 8 cut(s) 16, 113, 238, 384, 579, 802, 1276, 1527
DpnII GATC 8 cut(s) 14, 111, 236, 382, 577, 800, 1274, 1525
EaeI YGGCCR 1 cut(s) 1543
Eam1104I CTCTTC 6 cut(s) 624, 640, 1248, 1299, 1343, 1438
EarI CTCTTC 6 cut(s) 624, 640, 1248, 1299, 1343, 1438
Eco130I CCWWGG 2 cut(s) 495, 886
Eco24I GRGCYC 1 cut(s) 432
Eco81I CCTNAGG 1 cut(s) 1605
Eco91I GGTNACC 1 cut(s) 1609
EcoO65I GGTNACC 1 cut(s) 1609
EcoRII CCWGG 4 cut(s) 50, 198, 396, 996
EcoT14I CCWWGG 2 cut(s) 495, 886
EcoT22I ATGCAT 3 cut(s) 257, 775, 1419
EcoT38I GRGCYC 1 cut(s) 432
ErhI CCWWGG 2 cut(s) 495, 886
FauNDI CATATG 1 cut(s) 627
Fnu4HI GCNGC 4 cut(s) 63, 363, 366, 1293
FokI GGATG 3 cut(s) 7, 857, 1464
FriOI GRGCYC 1 cut(s) 432
Fsp4HI GCNGC 4 cut(s) 63, 363, 366, 1293
FspBI CTAG 4 cut(s) 938, 1259, 1407, 1482
GlaI GCGC 1 cut(s) 731
GluI GCNGC 4 cut(s) 63, 363, 366, 1293
HaeIII GGCC 3 cut(s) 167, 512, 1545
HapII CCGG 1 cut(s) 685
HgaI GACGC 1 cut(s) 1563
HhaI GCGC 1 cut(s) 732
Hin6I GCGC 1 cut(s) 730
HinP1I GCGC 1 cut(s) 730
HincII GTYRAC 2 cut(s) 715, 1061
HindII GTYRAC 2 cut(s) 715, 1061
HinfI GANTC 5 cut(s) 392, 834, 1078, 1410, 1474
HpaII CCGG 1 cut(s) 685
HphI GGTGA 4 cut(s) 268, 484, 1222, 1603
Hpy166II GTNNAC 6 cut(s) 32, 597, 706, 715, 759, 1061
Hpy188I TCNGA 2 cut(s) 552, 833
Hpy188III TCNNGA 9 cut(s) 38, 992, 1075, 1138, 1285, 1391, 1407, 1502, 1532
Hpy8I GTNNAC 6 cut(s) 32, 597, 706, 715, 759, 1061
HpyAV CCTTC 3 cut(s) 501, 1347, 1529
HpyCH4III ACNGT 8 cut(s) 226, 389, 556, 666, 737, 826, 1043, 1216
HpyCH4IV ACGT 1 cut(s) 1344
HpyF10VI GCNNNNNNNGC 6 cut(s) 17, 62, 436, 977, 1306, 1571
HpyF3I CTNAG 4 cut(s) 549, 1491, 1584, 1605
HpySE526I ACGT 1 cut(s) 1344
HspAI GCGC 1 cut(s) 730
Kzo9I GATC 8 cut(s) 14, 111, 236, 382, 577, 800, 1274, 1525
LguI GCTCTTC 2 cut(s) 624, 1299
Lsp1109I GCAGC 4 cut(s) 74, 374, 377, 1304
LweI GCATC 2 cut(s) 29, 1131
MaeI CTAG 4 cut(s) 938, 1259, 1407, 1482
MaeII ACGT 1 cut(s) 1344
MaeIII GTNAC 7 cut(s) 210, 1043, 1135, 1210, 1232, 1261, 1609
MalI GATC 8 cut(s) 16, 113, 238, 384, 579, 802, 1276, 1527
MboI GATC 8 cut(s) 14, 111, 236, 382, 577, 800, 1274, 1525
MboII GAAGA 8 cut(s) 611, 657, 673, 859, 1235, 1316, 1360, 1425
MflI RGATCY 1 cut(s) 236
MhlI GDGCHC 2 cut(s) 108, 432
MlsI TGGCCA 1 cut(s) 1545
MluNI TGGCCA 1 cut(s) 1545
MlyI GAGTC 2 cut(s) 1072, 1483
MmeI TCCRAC 1 cut(s) 811
Mox20I TGGCCA 1 cut(s) 1545
Mph1103I ATGCAT 3 cut(s) 257, 775, 1419
MscI TGGCCA 1 cut(s) 1545
MseI TTAA 4 cut(s) 221, 1011, 1451, 1627
Msp20I TGGCCA 1 cut(s) 1545
MspI CCGG 1 cut(s) 685
MspR9I CCNGG 4 cut(s) 52, 200, 398, 998
Mva1269I GAATGC 6 cut(s) 272, 360, 773, 1111, 1417, 1605
MvaI CCWGG 4 cut(s) 52, 200, 398, 998
MwoI GCNNNNNNNGC 6 cut(s) 17, 62, 436, 977, 1306, 1571
NcoI CCATGG 2 cut(s) 495, 886
NdeI CATATG 1 cut(s) 627
NdeII GATC 8 cut(s) 14, 111, 236, 382, 577, 800, 1274, 1525
NlaIV GGNNCC 2 cut(s) 652, 949
NmuCI GTSAC 6 cut(s) 210, 1043, 1135, 1210, 1261, 1609
NsiI ATGCAT 3 cut(s) 257, 775, 1419
PagI TCATGA 3 cut(s) 991, 1390, 1501
PaqCI CACCTGC 1 cut(s) 886
PasI CCCWGGG 1 cut(s) 397
PciSI GCTCTTC 2 cut(s) 624, 1299
PctI GAATGC 6 cut(s) 272, 360, 773, 1111, 1417, 1605
PfeI GAWTC 3 cut(s) 392, 834, 1410
Pfl23II CGTACG 1 cut(s) 1192
PkrI GCNGC 4 cut(s) 64, 364, 367, 1294
PleI GAGTC 2 cut(s) 1072, 1482
PpsI GAGTC 2 cut(s) 1072, 1482
PsiI TTATAA 2 cut(s) 1187, 1325
Psp6I CCWGG 4 cut(s) 50, 198, 396, 996
PspEI GGTNACC 1 cut(s) 1609
PspGI CCWGG 4 cut(s) 50, 198, 396, 996
PspLI CGTACG 1 cut(s) 1192
PspN4I GGNNCC 2 cut(s) 652, 949
PspPI GGNCC 1 cut(s) 510
PstI CTGCAG 2 cut(s) 13, 293
PsuI RGATCY 1 cut(s) 236
RsaI GTAC 4 cut(s) 640, 791, 1194, 1590
RsaNI GTAC 4 cut(s) 639, 790, 1193, 1589
SapI GCTCTTC 2 cut(s) 624, 1299
SaqAI TTAA 4 cut(s) 221, 1011, 1451, 1627
SatI GCNGC 4 cut(s) 63, 363, 366, 1293
Sau3AI GATC 8 cut(s) 14, 111, 236, 382, 577, 800, 1274, 1525
Sau96I GGNCC 1 cut(s) 510
SchI GAGTC 2 cut(s) 1072, 1483
ScrFI CCNGG 4 cut(s) 52, 200, 398, 998
SduI GDGCHC 2 cut(s) 108, 432
SfaNI GCATC 2 cut(s) 29, 1131
SfcI CTRYAG 3 cut(s) 9, 289, 825
SmlI CTYRAG 1 cut(s) 420
SmoI CTYRAG 1 cut(s) 420
SsiI CCGC 3 cut(s) 153, 1064, 1208
SspMI CTAG 4 cut(s) 938, 1259, 1407, 1482
StyD4I CCNGG 4 cut(s) 50, 198, 396, 996
StyI CCWWGG 2 cut(s) 495, 886
TaaI ACNGT 8 cut(s) 226, 389, 556, 666, 737, 826, 1043, 1216
TaiI ACGT 1 cut(s) 1347
TaqI TCGA 3 cut(s) 162, 622, 777
TatI WGTACW 2 cut(s) 638, 789
TfiI GAWTC 3 cut(s) 392, 834, 1410
Tru1I TTAA 4 cut(s) 221, 1011, 1451, 1627
Tru9I TTAA 4 cut(s) 221, 1011, 1451, 1627
TscAI CASTG 9 cut(s) 192, 382, 392, 561, 661, 708, 810, 1302, 1337
TseFI GTSAC 6 cut(s) 210, 1043, 1135, 1210, 1261, 1609
TseI GCWGC 4 cut(s) 62, 362, 365, 1292
Tsp45I GTSAC 6 cut(s) 210, 1043, 1135, 1210, 1261, 1609
TspDTI ATGAA 9 cut(s) 70, 439, 859, 980, 1118, 1185, 1481, 1490, 1566
TspRI CASTG 9 cut(s) 192, 382, 392, 561, 661, 708, 810, 1302, 1337
XapI RAATTY 3 cut(s) 1279, 1399, 1495
XbaI TCTAGA 1 cut(s) 1406
XcmI CCANNNNNNNNNTGG 1 cut(s) 55
XspI CTAG 4 cut(s) 938, 1259, 1407, 1482
Zsp2I ATGCAT 3 cut(s) 257, 775, 1419
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.