Rw5G036870

MLO-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0002520
Physical Location & Seq
Forward (+)
64750342 .. 64755498
5157 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G036870.1

Sequence Viewer

Length: 1689 bp
ATGGCTTCCGAAGGAACAAGTCGCTCCCTGCAAGACACACCCAGTTGGGCTCTGGCAACAGTTTGCTTCGTCTTCATCTTTCTCTCTCTTTTCATAGAGCACTTGATCCATCTCCTTTCCCAATTTCTCAAAAAGCATAGAAAGACTGCATTGTTCGAGGCCGTAGAGAAGCTCAAATCAGTGCTTATTTTTCTGGGTTTCATGTCCCTAATACTGACAGTAACACAGAGATGGATAGCCAATATTTGCATACCCACAGAAGTTGCAAATACTATGCTTCCTTGCAGAAAATCAGTATCAACTAAAACAACCAAAGCAGTTGGTTTCGATCGCATGTGGAGCACCGGGGCATCTACAGCTTTTGAAGTAATACAAAGAAGACTAGCAGAAACAGAAGATAGTAGTACTACTAGTTCCACTTCTACCACTTCTGTGGATGTGGATCGGTGCGAATCCCAGGGGAAGACCTCATTTATATCACAAGGGGGACTAAATCAGCTCAACAACTTCATCTTTGTTCTGGCAATTATGCAGATCGTGTACAGTGTTCTGACAATGGCCTTAGGAAGAGCCAAGATGAAACGTTGGGAAGCGTGGGAAAGAGAAACTCAGACGGTGGAATACCAAGTCGCAAATGATCCTAACCGATTCAGGTTTACAAGGCAGACGACATTTGCTCGTAGACACATGAATTCTTGTACACGAACACCTATTCAACTGTGGATTAAGTGCTTCTTCAGACAATTCTTCCACTCAGTTGAAAAAATTGATTATCTCACATTACGCCATGGCTTCATTTCTACTCATTTGTCAACAAACAACGCATTCAATTTCCAGCAGTACATAGAACGGTCACTGGAGGATGATTTCAGAGAAGTAGTTGGCATAAGCCCTTTCATGTGGTTTATCGTCGTAATCTTCATTCTCGTAGACGTGCATGGTTGGCAGGTGTATCTCTGGGTGTCATTTGTGCCACTAATCATTGTGCTAGTTCTTGGAACCAAACTGGAAGTGGTGGTAGCCAGAATGGCTCACCAACTCGGTGACCAGCATAATGTAATCAAAGGAACTCCTCTGGTTCAACCAAAGGACAGTCACTTCTGGTTCAATAGACCACGGTTCGTCTTGACTCTCGTACATCTCACACTGTTCATGAATGCATTTGAACTTGCTTTCTTCATTTGGGTCACGTTACAATTTGGGATACACTCTTGCTACCATGAACACATAGAGATTATCATTATAAGGGTGGTCTTAGCGGTTGTCGTTCAAGTTATGTGCAGTTACATTACTCTTCCCCTCTATGCTCTAGTGACCCAGATGGGGTCAAATTTGAAGAGTACAGCAGTGCTTGAAGAGCAAACAACAAAAGTTATAAAGCAGTGGCATGCGGACGTGAAGCAAAAGAGGAAAAAGAATAGGCAAGACAATTCAGAATCTGGTCACGATTATTCCTCTACCGTAGGAAGCAGTCAGAGAACCATGAATTCCCCAGATTTCTCTTCCCACCACCGGCACCCAAGCTTTGCTGAAATGTCCCAAATGGAGATTGTCGAAGATGGTCAAGAAATTGTTGAAGAACATCAAGAAAAGGGCAGCCAAAATGAAATTGAACTGGCTAGTAGTAGTACTGTTTTAGCTACTGAGGTACACATAGAAATTGCGGAGGTGAACAGAAGCCAAACGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0006952 GO:0008150 GO:0016020 GO:0044464 GO:0050896 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mlo

Protein Analysis

562

Amino Acids

64.54

Weight (kDa)

7.72

Isoelectric Point (pI)

44.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mlo PF03094 8 - 496 2.2e-196 Mlo family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1244, 1376
AarI CACCTGC 1 cut(s) 937
Acc36I ACCTGC 1 cut(s) 937
AccB1I GGYRCC 1 cut(s) 1515
AccI GTMKAC 2 cut(s) 682, 930
AciI CCGC 3 cut(s) 1259, 1391, 1664
AclI AACGTT 1 cut(s) 583
AclWI GGATC 3 cut(s) 100, 450, 632
AcsI RAATTY 3 cut(s) 691, 1330, 1486
AcuI CTGAAG 1 cut(s) 721
AfaI GTAC 8 cut(s) 406, 542, 700, 842, 1137, 1342, 1630, 1650
AfiI CCNNNNNNNGG 2 cut(s) 1323, 1512
AhlI ACTAGT 1 cut(s) 410
AjiI CACGTC 2 cut(s) 934, 1396
AjnI CCWGG 1 cut(s) 456
AleI CACNNNNGTG 1 cut(s) 431
AluBI AGCT 5 cut(s) 172, 359, 499, 1524, 1640
AluI AGCT 5 cut(s) 172, 359, 499, 1524, 1640
Alw21I GWGCWC 2 cut(s) 102, 344
AlwI GGATC 3 cut(s) 100, 450, 632
AlwNI CAGNNNCTG 1 cut(s) 1439
AoxI GGCC 2 cut(s) 159, 558
ApeKI GCWGC 1 cut(s) 1596
ApoI RAATTY 3 cut(s) 691, 1330, 1486
AsuC2I CCSGG 1 cut(s) 346
AsuHPI GGTGA 3 cut(s) 1025, 1055, 1681
AxyI CCTNAGG 1 cut(s) 562
BanI GGYRCC 1 cut(s) 1515
BanII GRGCYC 1 cut(s) 52
BbsI GAAGAC 3 cut(s) 64, 385, 470
Bbv12I GWGCWC 2 cut(s) 102, 344
BbvI GCAGC 1 cut(s) 1608
BccI CCATC 4 cut(s) 117, 225, 1315, 1553
BceAI ACGGC 1 cut(s) 146
BciT130I CCWGG 1 cut(s) 458
BciVI GTATCC 1 cut(s) 1197
BcnI CCSGG 1 cut(s) 346
BcuI ACTAGT 1 cut(s) 410
BfaI CTAG 5 cut(s) 383, 411, 989, 1310, 1620
BfmI CTRYAG 1 cut(s) 354
BfuAI ACCTGC 1 cut(s) 937
BfuI GTATCC 1 cut(s) 1197
BglI GCCNNNNNGGC 1 cut(s) 1028
BisI GCNGC 1 cut(s) 1597
BlsI GCNGC 1 cut(s) 1598
BmcAI AGTACT 2 cut(s) 406, 1630
Bme1390I CCNGG 2 cut(s) 346, 458
BmgBI CACGTC 2 cut(s) 934, 1396
BmiI GGNNCC 2 cut(s) 1000, 1517
BmrFI CCNGG 2 cut(s) 346, 458
BmrI ACTGGG 1 cut(s) 36
BmsI GCATC 1 cut(s) 359
BmuI ACTGGG 1 cut(s) 36
BpiI GAAGAC 3 cut(s) 64, 385, 470
BpmI CTGGAG 1 cut(s) 878
BpuMI CCSGG 1 cut(s) 346
BsaBI GATNNNNATC 1 cut(s) 441
BsaJI CCNNGG 5 cut(s) 345, 456, 457, 787, 1115
Bsc4I CCNNNNNNNGG 2 cut(s) 1323, 1512
Bse118I RCCGGY 1 cut(s) 1512
Bse1I ACTGG 4 cut(s) 42, 861, 1011, 1620
Bse21I CCTNAGG 1 cut(s) 562
Bse8I GATNNNNATC 1 cut(s) 441
BseBI CCWGG 1 cut(s) 458
BseDI CCNNGG 5 cut(s) 345, 456, 457, 787, 1115
BseGI GGATG 2 cut(s) 442, 868
BseJI GATNNNNATC 1 cut(s) 441
BseLI CCNNNNNNNGG 2 cut(s) 1323, 1512
BseMII CTCAG 3 cut(s) 623, 768, 1635
BseNI ACTGG 4 cut(s) 42, 861, 1011, 1620
BseRI GAGGAG 1 cut(s) 1062
BseXI GCAGC 1 cut(s) 1608
BsgI GTGCAG 1 cut(s) 1300
Bsh1285I CGRYCG 1 cut(s) 331
BshFI GGCC 2 cut(s) 161, 560
BshNI GGYRCC 1 cut(s) 1515
BsiEI CGRYCG 1 cut(s) 331
BsiHKAI GWGCWC 2 cut(s) 102, 344
BsiSI CCGG 2 cut(s) 345, 1513
BslFI GGGAC 3 cut(s) 190, 501, 1522
BslI CCNNNNNNNGG 2 cut(s) 1323, 1512
BsmFI GGGAC 3 cut(s) 190, 501, 1522
BsmI GAATGC 2 cut(s) 824, 1162
BsnI GGCC 2 cut(s) 161, 560
Bsp1286I GDGCHC 3 cut(s) 52, 102, 344
Bsp1407I TGTACA 2 cut(s) 540, 698
Bsp143I GATC 5 cut(s) 105, 328, 442, 534, 637
Bsp19I CCATGG 1 cut(s) 787
BspACI CCGC 3 cut(s) 1259, 1391, 1664
BspANI GGCC 2 cut(s) 161, 560
BspCNI CTCAG 3 cut(s) 622, 767, 1636
BspHI TCATGA 1 cut(s) 1152
BspLI GGNNCC 2 cut(s) 1000, 1517
BspMI ACCTGC 1 cut(s) 937
BspPI GGATC 3 cut(s) 100, 450, 632
BspQI GCTCTTC 2 cut(s) 562, 1350
BspT107I GGYRCC 1 cut(s) 1515
BsrFI RCCGGY 1 cut(s) 1512
BsrGI TGTACA 2 cut(s) 540, 698
BsrI ACTGG 4 cut(s) 42, 861, 1011, 1620
BssAI RCCGGY 1 cut(s) 1512
BssECI CCNNGG 5 cut(s) 345, 456, 457, 787, 1115
BssMI GATC 5 cut(s) 105, 328, 442, 534, 637
BssT1I CCWWGG 1 cut(s) 787
Bst2UI CCWGG 1 cut(s) 458
Bst6I CTCTTC 5 cut(s) 562, 1299, 1331, 1350, 1507
BstAUI TGTACA 2 cut(s) 540, 698
BstC8I GCNNGC 1 cut(s) 1389
BstDEI CTNAG 5 cut(s) 562, 609, 754, 1255, 1644
BstDSI CCRYGG 2 cut(s) 787, 1115
BstEII GGTNACC 1 cut(s) 1043
BstF5I GGATG 2 cut(s) 442, 868
BstKTI GATC 5 cut(s) 108, 331, 445, 537, 640
BstMBI GATC 5 cut(s) 105, 328, 442, 534, 637
BstMCI CGRYCG 1 cut(s) 331
BstMWI GCNNNNNNNGC 5 cut(s) 339, 356, 943, 1028, 1357
BstNI CCWGG 1 cut(s) 458
BstNSI RCATGY 2 cut(s) 337, 1391
BstPI GGTNACC 1 cut(s) 1043
BstSCI CCNGG 2 cut(s) 344, 456
BstSFI CTRYAG 1 cut(s) 354
BstV1I GCAGC 1 cut(s) 1608
BstV2I GAAGAC 3 cut(s) 64, 385, 470
BstXI CCANNNNNNTGG 1 cut(s) 433
Bsu36I CCTNAGG 1 cut(s) 562
BsuI GTATCC 1 cut(s) 1197
BsuRI GGCC 2 cut(s) 161, 560
BtgI CCRYGG 2 cut(s) 787, 1115
BtrI CACGTC 2 cut(s) 934, 1396
BtsCI GGATG 2 cut(s) 442, 868
BtsI GCAGTG 2 cut(s) 1353, 1388
BtsIMutI CAGTG 6 cut(s) 186, 550, 854, 1145, 1353, 1388
BveI ACCTGC 1 cut(s) 937
Cac8I GCNNGC 1 cut(s) 1389
CaiI CAGNNNCTG 1 cut(s) 1439
CciI TCATGA 1 cut(s) 1152
Cfr10I RCCGGY 1 cut(s) 1512
Csp6I GTAC 8 cut(s) 405, 541, 699, 841, 1136, 1341, 1629, 1649
CspCI CAANNNNNGTGG 2 cut(s) 244, 279
CviQI GTAC 8 cut(s) 405, 541, 699, 841, 1136, 1341, 1629, 1649
DdeI CTNAG 5 cut(s) 562, 609, 754, 1255, 1644
DpnI GATC 5 cut(s) 107, 330, 444, 536, 639
DpnII GATC 5 cut(s) 105, 328, 442, 534, 637
Eam1104I CTCTTC 5 cut(s) 562, 1299, 1331, 1350, 1507
EarI CTCTTC 5 cut(s) 562, 1299, 1331, 1350, 1507
Eco130I CCWWGG 1 cut(s) 787
Eco24I GRGCYC 1 cut(s) 52
Eco57I CTGAAG 1 cut(s) 721
Eco81I CCTNAGG 1 cut(s) 562
Eco91I GGTNACC 1 cut(s) 1043
EcoO65I GGTNACC 1 cut(s) 1043
EcoRI GAATTC 2 cut(s) 691, 1486
EcoRII CCWGG 1 cut(s) 456
EcoT14I CCWWGG 1 cut(s) 787
EcoT22I ATGCAT 1 cut(s) 1162
EcoT38I GRGCYC 1 cut(s) 52
ErhI CCWWGG 1 cut(s) 787
FalI AAGNNNNNCTT 2 cut(s) 719, 751
FaqI GGGAC 3 cut(s) 190, 501, 1522
FblI GTMKAC 2 cut(s) 682, 930
Fnu4HI GCNGC 1 cut(s) 1597
FokI GGATG 2 cut(s) 449, 875
FriOI GRGCYC 1 cut(s) 52
Fsp4HI GCNGC 1 cut(s) 1597
FspBI CTAG 5 cut(s) 383, 411, 989, 1310, 1620
GluI GCNGC 1 cut(s) 1597
GsuI CTGGAG 1 cut(s) 878
HaeIII GGCC 2 cut(s) 161, 560
HapII CCGG 2 cut(s) 345, 1513
HincII GTYRAC 1 cut(s) 813
HindII GTYRAC 1 cut(s) 813
HindIII AAGCTT 1 cut(s) 1522
HinfI GANTC 4 cut(s) 452, 648, 1129, 1436
HpaII CCGG 2 cut(s) 345, 1513
HphI GGTGA 3 cut(s) 1025, 1055, 1681
Hpy166II GTNNAC 8 cut(s) 541, 657, 683, 701, 813, 931, 1651, 1672
Hpy188I TCNGA 7 cut(s) 10, 552, 612, 740, 872, 1435, 1476
Hpy188III TCNNGA 5 cut(s) 1126, 1153, 1445, 1565, 1586
Hpy8I GTNNAC 8 cut(s) 541, 657, 683, 701, 813, 931, 1651, 1672
Hpy99I CGWCG 1 cut(s) 914
HpyAV CCTTC 1 cut(s) 5
HpyCH4IV ACGT 5 cut(s) 583, 933, 1190, 1395, 1685
HpyCH4V TGCA 9 cut(s) 31, 149, 249, 266, 285, 532, 937, 1160, 1281
HpyF10VI GCNNNNNNNGC 5 cut(s) 339, 356, 943, 1028, 1357
HpyF3I CTNAG 5 cut(s) 562, 609, 754, 1255, 1644
HpySE526I ACGT 5 cut(s) 583, 933, 1190, 1395, 1685
Kzo9I GATC 5 cut(s) 105, 328, 442, 534, 637
LguI GCTCTTC 2 cut(s) 562, 1350
LmnI GCTCC 2 cut(s) 29, 339
Lsp1109I GCAGC 1 cut(s) 1608
LweI GCATC 1 cut(s) 359
MaeI CTAG 5 cut(s) 383, 411, 989, 1310, 1620
MaeII ACGT 5 cut(s) 583, 933, 1190, 1395, 1685
MaeIII GTNAC 9 cut(s) 220, 852, 1043, 1094, 1186, 1191, 1283, 1312, 1442
MalI GATC 5 cut(s) 107, 330, 444, 536, 639
MboI GATC 5 cut(s) 105, 328, 442, 534, 637
MhlI GDGCHC 3 cut(s) 52, 102, 344
MlyI GAGTC 1 cut(s) 1123
MnlI CCTC 9 cut(s) 151, 478, 853, 1083, 1310, 1401, 1465, 1639, 1660
Mph1103I ATGCAT 1 cut(s) 1162
MseI TTAA 1 cut(s) 726
MslI CAYNNNNRTG 2 cut(s) 229, 431
MspI CCGG 2 cut(s) 345, 1513
MspR9I CCNGG 2 cut(s) 346, 458
Mva1269I GAATGC 2 cut(s) 824, 1162
MvaI CCWGG 1 cut(s) 458
MwoI GCNNNNNNNGC 5 cut(s) 339, 356, 943, 1028, 1357
NciI CCSGG 1 cut(s) 346
NcoI CCATGG 1 cut(s) 787
NdeII GATC 5 cut(s) 105, 328, 442, 534, 637
NlaIV GGNNCC 2 cut(s) 1000, 1517
NmuCI GTSAC 6 cut(s) 852, 1043, 1094, 1186, 1312, 1442
NsiI ATGCAT 1 cut(s) 1162
NspI RCATGY 2 cut(s) 337, 1391
OliI CACNNNNGTG 1 cut(s) 431
PaeI GCATGC 1 cut(s) 1391
PagI TCATGA 1 cut(s) 1152
PaqCI CACCTGC 1 cut(s) 937
PasI CCCWGGG 1 cut(s) 457
PciSI GCTCTTC 2 cut(s) 562, 1350
PctI GAATGC 2 cut(s) 824, 1162
PfeI GAWTC 3 cut(s) 452, 648, 1436
PkrI GCNGC 1 cut(s) 1598
Ple19I CGATCG 1 cut(s) 331
PleI GAGTC 1 cut(s) 1123
PpsI GAGTC 1 cut(s) 1123
PsiI TTATAA 2 cut(s) 1244, 1376
Psp1406I AACGTT 1 cut(s) 583
Psp6I CCWGG 1 cut(s) 456
PspEI GGTNACC 1 cut(s) 1043
PspGI CCWGG 1 cut(s) 456
PspN4I GGNNCC 2 cut(s) 1000, 1517
PsrI GAACNNNNNNTAC 2 cut(s) 397, 429
PstNI CAGNNNCTG 1 cut(s) 1439
PvuI CGATCG 1 cut(s) 331
RsaI GTAC 8 cut(s) 406, 542, 700, 842, 1137, 1342, 1630, 1650
RsaNI GTAC 8 cut(s) 405, 541, 699, 841, 1136, 1341, 1629, 1649
RseI CAYNNNNRTG 2 cut(s) 229, 431
SapI GCTCTTC 2 cut(s) 562, 1350
SaqAI TTAA 1 cut(s) 726
SatI GCNGC 1 cut(s) 1597
Sau3AI GATC 5 cut(s) 105, 328, 442, 534, 637
ScaI AGTACT 2 cut(s) 406, 1630
SchI GAGTC 1 cut(s) 1123
ScrFI CCNGG 2 cut(s) 346, 458
SduI GDGCHC 3 cut(s) 52, 102, 344
SfaNI GCATC 1 cut(s) 359
SfcI CTRYAG 1 cut(s) 354
SmiMI CAYNNNNRTG 2 cut(s) 229, 431
SpeI ACTAGT 1 cut(s) 410
SphI GCATGC 1 cut(s) 1391
SsiI CCGC 3 cut(s) 1259, 1391, 1664
SspI AATATT 1 cut(s) 244
SspMI CTAG 5 cut(s) 383, 411, 989, 1310, 1620
StyD4I CCNGG 2 cut(s) 344, 456
StyI CCWWGG 1 cut(s) 787
TaiI ACGT 5 cut(s) 586, 936, 1193, 1398, 1688
TaqI TCGA 3 cut(s) 156, 327, 1554
TatI WGTACW 6 cut(s) 404, 540, 698, 840, 1340, 1628
TfiI GAWTC 3 cut(s) 452, 648, 1436
Tru1I TTAA 1 cut(s) 726
Tru9I TTAA 1 cut(s) 726
TscAI CASTG 6 cut(s) 186, 550, 861, 1152, 1353, 1388
TseFI GTSAC 6 cut(s) 852, 1043, 1094, 1186, 1312, 1442
TseI GCWGC 1 cut(s) 1596
Tsp45I GTSAC 6 cut(s) 852, 1043, 1094, 1186, 1312, 1442
TspRI CASTG 6 cut(s) 186, 550, 861, 1152, 1353, 1388
XapI RAATTY 3 cut(s) 691, 1330, 1486
XceI RCATGY 2 cut(s) 337, 1391
XcmI CCANNNNNNNNNTGG 2 cut(s) 49, 1009
XmiI GTMKAC 2 cut(s) 682, 930
XspI CTAG 5 cut(s) 383, 411, 989, 1310, 1620
ZrmI AGTACT 2 cut(s) 406, 1630
Zsp2I ATGCAT 1 cut(s) 1162
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.