MD12G1121200.v1.1

MLO-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0002520
Physical Location & Seq
Forward (+)
19428139 .. 19432501
4363 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1121200.v1.1.491

Sequence Viewer

Length: 1641 bp
ATGGCAGGAGACTCCAAAGGCGCCTCTCTGGAATACACTCCGACATGGGCACTCGCCACCATTTGCTTCGTCTTCATCTTCACCTCCCTTTTCATCGAGCACTCCATCCATCTCCTTGTCAAGTTTCTCAAAAGGCACAGGAAAACCGCCTTGATGGACGCGGTGGAGAAGCTCAAATCAGAGTTGATGCTTATGGGATTTGTGTCACTTTTGCTGGCAATAACCCAAAATTCGATATCTAAAATCTGTATATCTGCAAAGGCGGGAAACATCATGCTTCCATGTCGCAGAAATGAGACAACCAAAGAAGAAGAAGATGTCGAGAAATTTGTGATTGTGAATAAGATTGTGACAAACTTTTCGACCGGCGCAAATAGTTTGTATGATGAAATACTAAGTCAAATATCAGCGCATAGGCGACTTGCAGAGGAGGATGCAGATGCTACAGGTTCTTGCGGCGAGGGAAAAGTACCTTTCATGACGAAAGATGCTCTTCATCAGCTTCACATACTTATTTTTGCGCTGGCGGTTATGCATATTGTGTATAGTGTTCTTACCATGGGTTTGGGGAGGGCCAAGATGAGGCGCTGGGAAGCTTGGGAGCAAGAAACTCGAACAATGGAGTACCAAGTTGAGAATGATCCCAACCGGTTCAGGATTACCAGGCAAACAACATTTGGAAGAAGGCACATGAATTCTAGTTCAGAAACGTCATTGAATCTGTGGATGAGATGTTTTTTGAGGCAATTCCATAACTCAGTGGCAAAAGTTGACTACATCACCATGAGACATGGCTTCATAGCGGCTCATTTGCCAAGTAGGCAAAGCTTTGATTTCCAAAAATACATACAGCGATCTTTGGAGGAAGATTTCAAGATCATGGTTGCAATCAGCCCTTTAATGTGGTTTGCAGTAGCAATTCTCATGCTCCTCGACGTGTATGGTTGGTATGTATATCTCTGGCTTTCCTATGTTCCACTCTTGGTCGTTCTGGTTCTAGGAACCAAACTTGAGTATATCGTAGCAAAAATGGCTCTTCAAATTAAAGACCAGAACAGTGTGATCGTTGGAACACCCCTTGTGCGAGTAAACGACGATCTCTTCTGGTTCAGGAAACCTAGATTTGTCTTGGTGCTGCTACATTATACTCTATTTTTGAATGCATTTGAGCTGGCCTTCTTCATTTGGGTTACAATCCAATTCGGGTTCACATCGTGCTACCACGAGCACACTGTGGTCATTATCACGAGGATTTCCTTAGCGGTGGCTACTCAAGTCCTCTGCAGCTACATCACTCTTCCTCTCTATGCACTTGTCACACAGATGGGTTCACAGTTCAAGGGTAAGATTATGGAAGATAACATGGCAGACATTTTAAAGCAATGGCATGCTGAGGTGAGGAATAGAAGGAGAAGGGAACAACAAACTCCGCAATCAGCAAGAACATCTTTCTCCACAGATTGGAGCTCCGTGAGGAATAGCCTTTCAGAAATGCCTTCTTATATAAGAAGGATTTTCAAACCAGATGAACCAGCTGAAAACAGTGTCCAGTTGTCTAACAGAGGGAATGTACAAGACGAGATAGTAGAACAAGAGGGAAGCTCAAGGGGTTATAGAAGAAATGGAGATTATGGGGCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0006952 GO:0008150 GO:0016020 GO:0044464 GO:0050896 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mlo

Protein Analysis

547

Amino Acids

62.99

Weight (kDa)

8.97

Isoelectric Point (pI)

45.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mlo PF03094 8 - 487 2.7e-192 Mlo family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 20
AccB7I CCANNNNNTGG 1 cut(s) 1461
AccII CGCG 1 cut(s) 161
AciI CCGC 8 cut(s) 147, 161, 263, 456, 527, 803, 1262, 1430
AclWI GGATC 1 cut(s) 635
AcsI RAATTY 3 cut(s) 229, 326, 694
AcyI GRCGYC 1 cut(s) 21
AdeI CACNNNGTG 2 cut(s) 1215, 1234
AfaI GTAC 3 cut(s) 471, 626, 1572
AfiI CCNNNNNNNGG 2 cut(s) 582, 1461
AflIII ACRYGT 1 cut(s) 936
AgeI ACCGGT 1 cut(s) 648
AgsI TTSAA 6 cut(s) 718, 874, 1040, 1159, 1339, 1519
AjiI CACGTC 1 cut(s) 937
AjnI CCWGG 1 cut(s) 662
AluBI AGCT 9 cut(s) 172, 502, 596, 828, 1171, 1287, 1467, 1535, 1602
AluI AGCT 9 cut(s) 172, 502, 596, 828, 1171, 1287, 1467, 1535, 1602
Alw21I GWGCWC 3 cut(s) 102, 1230, 1469
Alw26I GTCTC 3 cut(s) 3, 290, 781
AlwI GGATC 1 cut(s) 635
AoxI GGCC 2 cut(s) 573, 1173
ApeKI GCWGC 2 cut(s) 1135, 1284
ApoI RAATTY 3 cut(s) 229, 326, 694
ArsI GACNNNNNNTTYG 2 cut(s) 343, 375
AsiGI ACCGGT 1 cut(s) 648
AspLEI GCGC 5 cut(s) 23, 371, 412, 523, 588
AspS9I GGNCC 1 cut(s) 573
AsuHPI GGTGA 3 cut(s) 73, 772, 1408
BaeGI GKGCMC 1 cut(s) 52
BanI GGYRCC 1 cut(s) 20
BanII GRGCYC 1 cut(s) 1469
BauI CACGAG 2 cut(s) 1223, 1246
BbsI GAAGAC 1 cut(s) 64
Bbv12I GWGCWC 3 cut(s) 102, 1230, 1469
BbvCI CCTCAGC 1 cut(s) 1392
BbvI GCAGC 2 cut(s) 1122, 1296
BccI CCATC 4 cut(s) 113, 117, 148, 1318
BcgI CGANNNNNNTGC 2 cut(s) 593, 627
BciT130I CCWGG 1 cut(s) 664
BcoDI GTCTC 3 cut(s) 3, 290, 781
BfaI CTAG 3 cut(s) 699, 998, 1119
BfmI CTRYAG 2 cut(s) 444, 1282
BfoI RGCGCY 2 cut(s) 24, 589
BglI GCCNNNNNGGC 1 cut(s) 820
BisI GCNGC 4 cut(s) 457, 804, 1136, 1285
BlsI GCNGC 4 cut(s) 458, 805, 1137, 1286
Bme1390I CCNGG 1 cut(s) 664
BmgBI CACGTC 1 cut(s) 937
BmgT120I GGNCC 1 cut(s) 573
BmiI GGNNCC 2 cut(s) 22, 1003
BmrFI CCNGG 1 cut(s) 664
BmsI GCATC 4 cut(s) 177, 424, 430, 478
BpiI GAAGAC 1 cut(s) 64
BplI GAGNNNNNCTC 2 cut(s) 1586, 1618
Bpu10I CCTNAGC 2 cut(s) 1258, 1392
BpuEI CTTGAG 3 cut(s) 1031, 1257, 1588
BsaHI GRCGYC 1 cut(s) 21
BsaJI CCNNGG 1 cut(s) 558
BsaWI WCCGGW 1 cut(s) 648
Bsc4I CCNNNNNNNGG 2 cut(s) 582, 1461
Bse118I RCCGGY 2 cut(s) 365, 648
Bse1I ACTGG 1 cut(s) 1549
Bse3DI GCAATG 1 cut(s) 1388
BseBI CCWGG 1 cut(s) 664
BseDI CCNNGG 1 cut(s) 558
BseGI GGATG 3 cut(s) 105, 439, 732
BseLI CCNNNNNNNGG 2 cut(s) 582, 1461
BseMI GCAATG 1 cut(s) 1388
BseMII CTCAG 2 cut(s) 771, 1383
BseNI ACTGG 1 cut(s) 1549
BseRI GAGGAG 2 cut(s) 443, 920
BseSI GKGCMC 1 cut(s) 52
BseXI GCAGC 2 cut(s) 1122, 1296
BseYI CCCAGC 1 cut(s) 588
Bsh1236I CGCG 1 cut(s) 161
Bsh1285I CGRYCG 1 cut(s) 366
BshFI GGCC 2 cut(s) 575, 1175
BshNI GGYRCC 1 cut(s) 20
BshTI ACCGGT 1 cut(s) 648
BsiEI CGRYCG 1 cut(s) 366
BsiHKAI GWGCWC 3 cut(s) 102, 1230, 1469
BsiSI CCGG 2 cut(s) 366, 649
BslI CCNNNNNNNGG 2 cut(s) 582, 1461
BsmAI GTCTC 3 cut(s) 3, 290, 781
BsmI GAATGC 1 cut(s) 1165
BsnI GGCC 2 cut(s) 575, 1175
Bsp1286I GDGCHC 4 cut(s) 52, 102, 1230, 1469
Bsp1407I TGTACA 1 cut(s) 1570
Bsp143I GATC 5 cut(s) 640, 854, 876, 1062, 1096
Bsp19I CCATGG 1 cut(s) 558
BspACI CCGC 8 cut(s) 147, 161, 263, 456, 527, 803, 1262, 1430
BspANI GGCC 2 cut(s) 575, 1175
BspCNI CTCAG 2 cut(s) 770, 1384
BspFNI CGCG 1 cut(s) 161
BspHI TCATGA 1 cut(s) 477
BspLI GGNNCC 2 cut(s) 22, 1003
BspMAI CTGCAG 1 cut(s) 1286
BspPI GGATC 1 cut(s) 635
BspQI GCTCTTC 2 cut(s) 498, 1041
BspT107I GGYRCC 1 cut(s) 20
BsrDI GCAATG 1 cut(s) 1388
BsrFI RCCGGY 2 cut(s) 365, 648
BsrGI TGTACA 1 cut(s) 1570
BsrI ACTGG 1 cut(s) 1549
BssAI RCCGGY 2 cut(s) 365, 648
BssECI CCNNGG 1 cut(s) 558
BssMI GATC 5 cut(s) 640, 854, 876, 1062, 1096
BssNI GRCGYC 1 cut(s) 21
BssSI CACGAG 2 cut(s) 1223, 1246
BssT1I CCWWGG 1 cut(s) 558
Bst2BI CACGAG 2 cut(s) 1223, 1246
Bst2UI CCWGG 1 cut(s) 664
Bst4CI ACNGT 4 cut(s) 1058, 1234, 1335, 1544
Bst6I CTCTTC 4 cut(s) 498, 1041, 1106, 1302
BstACI GRCGYC 1 cut(s) 21
BstAUI TGTACA 1 cut(s) 1570
BstC8I GCNNGC 4 cut(s) 216, 525, 1173, 1389
BstDEI CTNAG 4 cut(s) 395, 757, 1258, 1392
BstDSI CCRYGG 1 cut(s) 558
BstF5I GGATG 3 cut(s) 105, 439, 732
BstFNI CGCG 1 cut(s) 161
BstH2I RGCGCY 2 cut(s) 24, 589
BstHHI GCGC 5 cut(s) 23, 371, 412, 523, 588
BstKTI GATC 5 cut(s) 643, 857, 879, 1065, 1099
BstMAI GTCTC 3 cut(s) 3, 290, 781
BstMBI GATC 5 cut(s) 640, 854, 876, 1062, 1096
BstMCI CGRYCG 1 cut(s) 366
BstMWI GCNNNNNNNGC 2 cut(s) 820, 1031
BstNI CCWGG 1 cut(s) 664
BstNSI RCATGY 1 cut(s) 1391
BstSCI CCNGG 1 cut(s) 662
BstSFI CTRYAG 2 cut(s) 444, 1282
BstSLI GKGCMC 1 cut(s) 52
BstUI CGCG 1 cut(s) 161
BstV1I GCAGC 2 cut(s) 1122, 1296
BstV2I GAAGAC 1 cut(s) 64
BstXI CCANNNNNNTGG 1 cut(s) 565
BsuRI GGCC 2 cut(s) 575, 1175
BtgI CCRYGG 1 cut(s) 558
BtrI CACGTC 1 cut(s) 937
BtsCI GGATG 3 cut(s) 105, 439, 732
BtsIMutI CAGTG 4 cut(s) 765, 1063, 1230, 1549
Cac8I GCNNGC 4 cut(s) 216, 525, 1173, 1389
CciI TCATGA 1 cut(s) 477
CfoI GCGC 5 cut(s) 23, 371, 412, 523, 588
Cfr10I RCCGGY 2 cut(s) 365, 648
Cfr13I GGNCC 1 cut(s) 573
CseI GACGC 1 cut(s) 167
Csp6I GTAC 3 cut(s) 470, 625, 1571
CspAI ACCGGT 1 cut(s) 648
CviQI GTAC 3 cut(s) 470, 625, 1571
DdeI CTNAG 4 cut(s) 395, 757, 1258, 1392
DinI GGCGCC 1 cut(s) 22
DpnI GATC 5 cut(s) 642, 856, 878, 1064, 1098
DpnII GATC 5 cut(s) 640, 854, 876, 1062, 1096
DraI TTTAAA 1 cut(s) 1377
DraIII CACNNNGTG 2 cut(s) 1215, 1234
Eam1104I CTCTTC 4 cut(s) 498, 1041, 1106, 1302
EarI CTCTTC 4 cut(s) 498, 1041, 1106, 1302
Ecl136II GAGCTC 1 cut(s) 1467
Eco130I CCWWGG 1 cut(s) 558
Eco24I GRGCYC 1 cut(s) 1469
Eco32I GATATC 1 cut(s) 237
Eco53kI GAGCTC 1 cut(s) 1467
EcoICRI GAGCTC 1 cut(s) 1467
EcoRI GAATTC 1 cut(s) 694
EcoRII CCWGG 1 cut(s) 662
EcoRV GATATC 1 cut(s) 237
EcoT14I CCWWGG 1 cut(s) 558
EcoT22I ATGCAT 2 cut(s) 537, 1165
EcoT38I GRGCYC 1 cut(s) 1469
EgeI GGCGCC 1 cut(s) 22
EheI GGCGCC 1 cut(s) 22
ErhI CCWWGG 1 cut(s) 558
FalI AAGNNNNNCTT 4 cut(s) 477, 509, 1432, 1464
FauI CCCGC 1 cut(s) 256
Fnu4HI GCNGC 4 cut(s) 457, 804, 1136, 1285
FokI GGATG 3 cut(s) 92, 446, 739
FriOI GRGCYC 1 cut(s) 1469
Fsp4HI GCNGC 4 cut(s) 457, 804, 1136, 1285
FspBI CTAG 3 cut(s) 699, 998, 1119
GlaI GCGC 5 cut(s) 22, 370, 411, 522, 587
GluI GCNGC 4 cut(s) 457, 804, 1136, 1285
GsaI CCCAGC 1 cut(s) 592
HaeII RGCGCY 2 cut(s) 24, 589
HaeIII GGCC 2 cut(s) 575, 1175
HapII CCGG 2 cut(s) 366, 649
HgaI GACGC 1 cut(s) 167
HhaI GCGC 5 cut(s) 23, 371, 412, 523, 588
Hin1I GRCGYC 1 cut(s) 21
Hin6I GCGC 5 cut(s) 21, 369, 410, 521, 586
HinP1I GCGC 5 cut(s) 21, 369, 410, 521, 586
HincII GTYRAC 1 cut(s) 772
HindII GTYRAC 1 cut(s) 772
HindIII AAGCTT 2 cut(s) 594, 826
HinfI GANTC 2 cut(s) 11, 718
HpaII CCGG 2 cut(s) 366, 649
HphI GGTGA 3 cut(s) 73, 772, 1408
Hpy166II GTNNAC 4 cut(s) 772, 1090, 1209, 1331
Hpy188I TCNGA 4 cut(s) 42, 181, 706, 1489
Hpy188III TCNNGA 7 cut(s) 29, 322, 478, 655, 874, 1111, 1246
Hpy8I GTNNAC 4 cut(s) 772, 1090, 1209, 1331
Hpy99I CGWCG 2 cut(s) 938, 1097
HpyAV CCTTC 6 cut(s) 678, 1186, 1401, 1407, 1503, 1506
HpyCH4III ACNGT 4 cut(s) 1058, 1234, 1335, 1544
HpyCH4IV ACGT 2 cut(s) 710, 936
HpyCH4V TGCA 9 cut(s) 257, 425, 437, 535, 887, 911, 1163, 1284, 1310
HpyF10VI GCNNNNNNNGC 2 cut(s) 820, 1031
HpyF3I CTNAG 4 cut(s) 395, 757, 1258, 1392
HpySE526I ACGT 2 cut(s) 710, 936
Hsp92I GRCGYC 1 cut(s) 21
HspAI GCGC 5 cut(s) 21, 369, 410, 521, 586
KasI GGCGCC 1 cut(s) 20
Kzo9I GATC 5 cut(s) 640, 854, 876, 1062, 1096
LguI GCTCTTC 2 cut(s) 498, 1041
LmnI GCTCC 4 cut(s) 601, 933, 1464, 1472
Lsp1109I GCAGC 2 cut(s) 1122, 1296
LweI GCATC 4 cut(s) 177, 424, 430, 478
MaeI CTAG 3 cut(s) 699, 998, 1119
MaeII ACGT 2 cut(s) 710, 936
MaeIII GTNAC 4 cut(s) 204, 349, 1189, 1315
MalI GATC 5 cut(s) 642, 856, 878, 1064, 1098
MboI GATC 5 cut(s) 640, 854, 876, 1062, 1096
MhlI GDGCHC 4 cut(s) 52, 102, 1230, 1469
MluCI AATT 7 cut(s) 229, 326, 694, 746, 918, 1041, 1199
Mly113I GGCGCC 1 cut(s) 21
MlyI GAGTC 1 cut(s) 5
MmeI TCCRAC 2 cut(s) 65, 1048
Mph1103I ATGCAT 2 cut(s) 537, 1165
MseI TTAA 4 cut(s) 899, 1044, 1376, 1639
MslI CAYNNNNRTG 2 cut(s) 782, 1322
MspA1I CMGCKG 1 cut(s) 1535
MspI CCGG 2 cut(s) 366, 649
MspR9I CCNGG 1 cut(s) 664
Mva1269I GAATGC 1 cut(s) 1165
MvaI CCWGG 1 cut(s) 664
MvnI CGCG 1 cut(s) 161
MwoI GCNNNNNNNGC 2 cut(s) 820, 1031
NarI GGCGCC 1 cut(s) 21
NcoI CCATGG 1 cut(s) 558
NdeII GATC 5 cut(s) 640, 854, 876, 1062, 1096
NlaIV GGNNCC 2 cut(s) 22, 1003
NmuCI GTSAC 3 cut(s) 204, 349, 1315
NsiI ATGCAT 2 cut(s) 537, 1165
NspI RCATGY 1 cut(s) 1391
PaeI GCATGC 1 cut(s) 1391
PagI TCATGA 1 cut(s) 477
PciSI GCTCTTC 2 cut(s) 498, 1041
PctI GAATGC 1 cut(s) 1165
PfeI GAWTC 1 cut(s) 718
PflMI CCANNNNNTGG 1 cut(s) 1461
PinAI ACCGGT 1 cut(s) 648
PkrI GCNGC 4 cut(s) 458, 805, 1137, 1286
PleI GAGTC 1 cut(s) 5
PluTI GGCGCC 1 cut(s) 24
PpsI GAGTC 1 cut(s) 5
Psp124BI GAGCTC 1 cut(s) 1469
Psp6I CCWGG 1 cut(s) 662
PspFI CCCAGC 1 cut(s) 588
PspGI CCWGG 1 cut(s) 662
PspN4I GGNNCC 2 cut(s) 22, 1003
PspPI GGNCC 1 cut(s) 573
PstI CTGCAG 1 cut(s) 1286
PvuII CAGCTG 1 cut(s) 1535
RsaI GTAC 3 cut(s) 471, 626, 1572
RsaNI GTAC 3 cut(s) 470, 625, 1571
RseI CAYNNNNRTG 2 cut(s) 782, 1322
SacI GAGCTC 1 cut(s) 1469
SapI GCTCTTC 2 cut(s) 498, 1041
SaqAI TTAA 4 cut(s) 899, 1044, 1376, 1639
SatI GCNGC 4 cut(s) 457, 804, 1136, 1285
Sau3AI GATC 5 cut(s) 640, 854, 876, 1062, 1096
Sau96I GGNCC 1 cut(s) 573
SchI GAGTC 1 cut(s) 5
ScrFI CCNGG 1 cut(s) 664
SduI GDGCHC 4 cut(s) 52, 102, 1230, 1469
SfaNI GCATC 4 cut(s) 177, 424, 430, 478
SfcI CTRYAG 2 cut(s) 444, 1282
SfoI GGCGCC 1 cut(s) 22
SmiMI CAYNNNNRTG 2 cut(s) 782, 1322
SmlI CTYRAG 3 cut(s) 1010, 1272, 1603
SmoI CTYRAG 3 cut(s) 1010, 1272, 1603
SphI GCATGC 1 cut(s) 1391
Sse9I AATT 7 cut(s) 229, 326, 694, 746, 918, 1041, 1199
SsiI CCGC 8 cut(s) 147, 161, 263, 456, 527, 803, 1262, 1430
SspDI GGCGCC 1 cut(s) 20
SspMI CTAG 3 cut(s) 699, 998, 1119
SstI GAGCTC 1 cut(s) 1469
StyD4I CCNGG 1 cut(s) 662
StyI CCWWGG 1 cut(s) 558
TaaI ACNGT 4 cut(s) 1058, 1234, 1335, 1544
TaiI ACGT 2 cut(s) 713, 939
TaqI TCGA 6 cut(s) 96, 233, 321, 362, 613, 933
TasI AATT 7 cut(s) 229, 326, 694, 746, 918, 1041, 1199
TatI WGTACW 1 cut(s) 1570
TauI GCSGC 2 cut(s) 459, 806
TfiI GAWTC 1 cut(s) 718
Tru1I TTAA 4 cut(s) 899, 1044, 1376, 1639
Tru9I TTAA 4 cut(s) 899, 1044, 1376, 1639
TscAI CASTG 4 cut(s) 765, 1063, 1237, 1549
TseFI GTSAC 3 cut(s) 204, 349, 1315
TseI GCWGC 2 cut(s) 1135, 1284
Tsp45I GTSAC 3 cut(s) 204, 349, 1315
TspDTI ATGAA 9 cut(s) 64, 82, 402, 466, 485, 707, 787, 1171, 1542
TspGWI ACGGA 1 cut(s) 1459
TspRI CASTG 4 cut(s) 765, 1063, 1237, 1549
Van91I CCANNNNNTGG 1 cut(s) 1461
XapI RAATTY 3 cut(s) 229, 326, 694
XceI RCATGY 1 cut(s) 1391
XspI CTAG 3 cut(s) 699, 998, 1119
Zsp2I ATGCAT 2 cut(s) 537, 1165
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.