RchiOBHm_Chr5g0060001

MLO-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0002520
Physical Location & Seq
Forward (+)
65103860 .. 65109084
5225 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ33655

Sequence Viewer

Length: 1680 bp
ATGGCTTCCGAAGGAACAAGTCGCTCCCTGCAAGACACACCCAGTTGGGCTCTGGCAACAGTTTGCTTCGTCTTCATCTTTCTCTCTCTCTTCATAGAGCACTTGATCCATCTCCTTTCCCAATTTCTCAAAAAGCATAGAAAGACTGCATTGTTCGAGGCCGTAGAGAAGCTCAAATCAGTGCTTATTTTTCTGGGTTTCATGTCCCTAATACTGACAGTAACACAGAGATGGATAGCCAATATTTGCATACCCACAGAAGTTGCATATACTATGCTTCCTTGCAGAAAATCAGTATCAACTAAAACAACCAAAGCAGTTGGTTTCGATCGCATGTGGAGCACCGGGGCATCTACAACTTTTGAAGTAATACAAAGAAGACTAGCAGAAACAGAAGATAGTACTACTAGTTCCACTTCTACCACTTCTGAGGATCACTGCGAATCCCAGGGGAAGACCTCATTTATATCACAAGGGGGACTAAATCAGCTCAACAACTTCATCTTTGCTCTGGCAATTATGCAGATCGTGTACAGTGTTCTGACAATGGCCTTAGGAAGAGCCAAGATGAAGCGTTGGGAAGCGTGGGAAAGAGAAACTCAGACGGTGGATTACCAAGTCGCAAATGATCCTAACCGATTCAGGTTTACAAGGCAGACGACATTTGCTCGTAGACACATGAATTCTTGTACAGGAACACCTATTCAACTGTGGATTAAGTGCTTCTTCAGACAATTCTTCCACTCAGTTGAAAAAATTGATTATCTCACATTACGCCATGGCTTCATTTCTACTCATTTGTCAACAAACAACGCATTCAATTTCCAGCAGTACATAGAACGGTCGCTGGAGGATGATTTCAGAGAAGTAGTTGGCATAAGCCCTTTCATGTGGTTTATCGTCGTAATCTTCATTCTCGTAGACGTGCATGGTTGGCAGGTGTATCTCTGGGTGTCATTTGTGCCACTAATCATTGTGCTAGTTCTTGGAACCAAACTGGAAGTGGTGGTGGCCAGAATGGCTCACCAACTCGGTGACCAGCATAATGTAATCAAAGGAACTCCTCTGGTTCAACCAAAGGACAGTCACTTCTGGTTCAATAGACCGCGGTTCGTCTTGACTCTCGTACATCTCACACTGTTCATGAATGCATTTGAACTTGCTTTCTTCATTTGGGTCACGTTACAATTTGGGATACACTCTTGCTACCATGAACACATAGAGATTATCATTATAAGGGTGGTCTTAGCGGTTGTCGTTCAAGTTATGTGCAGTTACATTACTCTTCCCCTCTATGCTCTAGTGACCCAGATGGGGTCAAATTTGAAGAGTACAGCAGTGCTTGAAGAGCAAACAACAAAAGTTATAAAGCAGTGGCATGCGGACGTGAAGCAAAAGAGGAAAAAGAATAGGCAAGACAATTCAGAATCTGGTCACGATTATTCCTCTACTGTAGGAAGCAGTCAGAGAACCATGAATTCCCCAGATTTCTCTTCCCACCACCGGCACCCAACCTTTGCTGAAATGTCCCAAATGGAGATTGTCGAAGATGGTCAAGAAATTGTCGAAGAACATCAAGAAAAGGGCAGCCAAAATGAAATTGAACTGGCTAGTAGTAGTACTGTTTTAGCTACTGAGGTACACATAGAAATTTCGGAGGTGAACAGAAGCCAAACGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0006952 GO:0008150 GO:0016020 GO:0044464 GO:0050896 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mlo

Protein Analysis

559

Amino Acids

64.22

Weight (kDa)

7.1

Isoelectric Point (pI)

45.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mlo PF03094 8 - 493 1.5e-194 Mlo family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1235, 1367
AarI CACCTGC 1 cut(s) 928
Acc36I ACCTGC 1 cut(s) 928
AccB1I GGYRCC 1 cut(s) 1506
AccI GTMKAC 2 cut(s) 673, 921
AccII CGCG 1 cut(s) 1108
AciI CCGC 4 cut(s) 1106, 1108, 1250, 1382
AclWI GGATC 3 cut(s) 100, 441, 623
AcoI YGGCCR 1 cut(s) 1011
AcsI RAATTY 4 cut(s) 682, 1321, 1477, 1650
AcuI CTGAAG 1 cut(s) 712
AfaI GTAC 8 cut(s) 403, 533, 691, 833, 1128, 1333, 1621, 1641
AfiI CCNNNNNNNGG 2 cut(s) 1314, 1503
AhlI ACTAGT 1 cut(s) 407
AjiI CACGTC 2 cut(s) 925, 1387
AjnI CCWGG 1 cut(s) 447
AluBI AGCT 3 cut(s) 172, 490, 1631
AluI AGCT 3 cut(s) 172, 490, 1631
Alw21I GWGCWC 2 cut(s) 102, 344
AlwI GGATC 3 cut(s) 100, 441, 623
AlwNI CAGNNNCTG 1 cut(s) 1430
AoxI GGCC 3 cut(s) 159, 549, 1011
ApeKI GCWGC 1 cut(s) 1587
ApoI RAATTY 4 cut(s) 682, 1321, 1477, 1650
AsuC2I CCSGG 1 cut(s) 346
AsuHPI GGTGA 3 cut(s) 1016, 1046, 1672
AxyI CCTNAGG 1 cut(s) 553
BalI TGGCCA 1 cut(s) 1013
BanI GGYRCC 1 cut(s) 1506
BanII GRGCYC 1 cut(s) 52
BbsI GAAGAC 3 cut(s) 64, 385, 461
Bbv12I GWGCWC 2 cut(s) 102, 344
BbvI GCAGC 1 cut(s) 1599
BccI CCATC 4 cut(s) 117, 225, 1306, 1544
BceAI ACGGC 1 cut(s) 146
BciT130I CCWGG 1 cut(s) 449
BciVI GTATCC 1 cut(s) 1188
BcnI CCSGG 1 cut(s) 346
BcuI ACTAGT 1 cut(s) 407
BfaI CTAG 5 cut(s) 383, 408, 980, 1301, 1611
BfmI CTRYAG 1 cut(s) 1452
BfuAI ACCTGC 1 cut(s) 928
BfuI GTATCC 1 cut(s) 1188
BglI GCCNNNNNGGC 1 cut(s) 1019
BisI GCNGC 1 cut(s) 1588
BlsI GCNGC 1 cut(s) 1589
BmcAI AGTACT 2 cut(s) 403, 1621
Bme1390I CCNGG 2 cut(s) 346, 449
BmgBI CACGTC 2 cut(s) 925, 1387
BmiI GGNNCC 2 cut(s) 991, 1508
BmrFI CCNGG 2 cut(s) 346, 449
BmrI ACTGGG 1 cut(s) 36
BmsI GCATC 1 cut(s) 359
BmuI ACTGGG 1 cut(s) 36
BpiI GAAGAC 3 cut(s) 64, 385, 461
BpmI CTGGAG 1 cut(s) 869
BpuMI CCSGG 1 cut(s) 346
BsaJI CCNNGG 5 cut(s) 345, 447, 448, 778, 1106
Bsc4I CCNNNNNNNGG 2 cut(s) 1314, 1503
Bse118I RCCGGY 1 cut(s) 1503
Bse1I ACTGG 3 cut(s) 42, 1002, 1611
Bse21I CCTNAGG 1 cut(s) 553
BseBI CCWGG 1 cut(s) 449
BseDI CCNNGG 5 cut(s) 345, 447, 448, 778, 1106
BseGI GGATG 1 cut(s) 859
BseLI CCNNNNNNNGG 2 cut(s) 1314, 1503
BseMII CTCAG 4 cut(s) 420, 614, 759, 1626
BseNI ACTGG 3 cut(s) 42, 1002, 1611
BseRI GAGGAG 1 cut(s) 1053
BseXI GCAGC 1 cut(s) 1599
BsgI GTGCAG 1 cut(s) 1291
Bsh1236I CGCG 1 cut(s) 1108
Bsh1285I CGRYCG 2 cut(s) 331, 845
BshFI GGCC 3 cut(s) 161, 551, 1013
BshNI GGYRCC 1 cut(s) 1506
BsiEI CGRYCG 2 cut(s) 331, 845
BsiHKAI GWGCWC 2 cut(s) 102, 344
BsiSI CCGG 2 cut(s) 345, 1504
BslFI GGGAC 3 cut(s) 190, 492, 1513
BslI CCNNNNNNNGG 2 cut(s) 1314, 1503
BsmFI GGGAC 3 cut(s) 190, 492, 1513
BsmI GAATGC 2 cut(s) 815, 1153
BsnI GGCC 3 cut(s) 161, 551, 1013
Bsp1286I GDGCHC 3 cut(s) 52, 102, 344
Bsp1407I TGTACA 2 cut(s) 531, 689
Bsp143I GATC 5 cut(s) 105, 328, 433, 525, 628
Bsp19I CCATGG 1 cut(s) 778
BspACI CCGC 4 cut(s) 1106, 1108, 1250, 1382
BspANI GGCC 3 cut(s) 161, 551, 1013
BspCNI CTCAG 4 cut(s) 421, 613, 758, 1627
BspFNI CGCG 1 cut(s) 1108
BspHI TCATGA 1 cut(s) 1143
BspLI GGNNCC 2 cut(s) 991, 1508
BspMI ACCTGC 1 cut(s) 928
BspPI GGATC 3 cut(s) 100, 441, 623
BspQI GCTCTTC 2 cut(s) 553, 1341
BspT107I GGYRCC 1 cut(s) 1506
BsrFI RCCGGY 1 cut(s) 1503
BsrGI TGTACA 2 cut(s) 531, 689
BsrI ACTGG 3 cut(s) 42, 1002, 1611
BssAI RCCGGY 1 cut(s) 1503
BssECI CCNNGG 5 cut(s) 345, 447, 448, 778, 1106
BssMI GATC 5 cut(s) 105, 328, 433, 525, 628
BssT1I CCWWGG 1 cut(s) 778
Bst2UI CCWGG 1 cut(s) 449
Bst6I CTCTTC 6 cut(s) 95, 553, 1290, 1322, 1341, 1498
BstAUI TGTACA 2 cut(s) 531, 689
BstC8I GCNNGC 1 cut(s) 1380
BstDEI CTNAG 6 cut(s) 429, 553, 600, 745, 1246, 1635
BstDSI CCRYGG 2 cut(s) 778, 1106
BstEII GGTNACC 1 cut(s) 1034
BstF5I GGATG 1 cut(s) 859
BstFNI CGCG 1 cut(s) 1108
BstKTI GATC 5 cut(s) 108, 331, 436, 528, 631
BstMBI GATC 5 cut(s) 105, 328, 433, 525, 628
BstMCI CGRYCG 2 cut(s) 331, 845
BstMWI GCNNNNNNNGC 4 cut(s) 339, 934, 1019, 1348
BstNI CCWGG 1 cut(s) 449
BstNSI RCATGY 2 cut(s) 337, 1382
BstPI GGTNACC 1 cut(s) 1034
BstSCI CCNGG 2 cut(s) 344, 447
BstSFI CTRYAG 1 cut(s) 1452
BstUI CGCG 1 cut(s) 1108
BstV1I GCAGC 1 cut(s) 1599
BstV2I GAAGAC 3 cut(s) 64, 385, 461
Bsu36I CCTNAGG 1 cut(s) 553
BsuI GTATCC 1 cut(s) 1188
BsuRI GGCC 3 cut(s) 161, 551, 1013
BtgI CCRYGG 2 cut(s) 778, 1106
BtrI CACGTC 2 cut(s) 925, 1387
BtsCI GGATG 1 cut(s) 859
BtsI GCAGTG 3 cut(s) 436, 1344, 1379
BtsIMutI CAGTG 6 cut(s) 186, 436, 541, 1136, 1344, 1379
BveI ACCTGC 1 cut(s) 928
Cac8I GCNNGC 1 cut(s) 1380
CaiI CAGNNNCTG 1 cut(s) 1430
CciI TCATGA 1 cut(s) 1143
Cfr10I RCCGGY 1 cut(s) 1503
Cfr42I CCGCGG 1 cut(s) 1109
Csp6I GTAC 8 cut(s) 402, 532, 690, 832, 1127, 1332, 1620, 1640
CspCI CAANNNNNGTGG 2 cut(s) 244, 279
CviQI GTAC 8 cut(s) 402, 532, 690, 832, 1127, 1332, 1620, 1640
DdeI CTNAG 6 cut(s) 429, 553, 600, 745, 1246, 1635
DpnI GATC 5 cut(s) 107, 330, 435, 527, 630
DpnII GATC 5 cut(s) 105, 328, 433, 525, 628
EaeI YGGCCR 1 cut(s) 1011
Eam1104I CTCTTC 6 cut(s) 95, 553, 1290, 1322, 1341, 1498
EarI CTCTTC 6 cut(s) 95, 553, 1290, 1322, 1341, 1498
Eco130I CCWWGG 1 cut(s) 778
Eco24I GRGCYC 1 cut(s) 52
Eco57I CTGAAG 1 cut(s) 712
Eco81I CCTNAGG 1 cut(s) 553
Eco91I GGTNACC 1 cut(s) 1034
EcoO65I GGTNACC 1 cut(s) 1034
EcoRI GAATTC 2 cut(s) 682, 1477
EcoRII CCWGG 1 cut(s) 447
EcoT14I CCWWGG 1 cut(s) 778
EcoT22I ATGCAT 1 cut(s) 1153
EcoT38I GRGCYC 1 cut(s) 52
ErhI CCWWGG 1 cut(s) 778
FalI AAGNNNNNCTT 2 cut(s) 710, 742
FaqI GGGAC 3 cut(s) 190, 492, 1513
FblI GTMKAC 2 cut(s) 673, 921
Fnu4HI GCNGC 1 cut(s) 1588
FokI GGATG 1 cut(s) 866
FriOI GRGCYC 1 cut(s) 52
Fsp4HI GCNGC 1 cut(s) 1588
FspBI CTAG 5 cut(s) 383, 408, 980, 1301, 1611
GluI GCNGC 1 cut(s) 1588
GsuI CTGGAG 1 cut(s) 869
HaeIII GGCC 3 cut(s) 161, 551, 1013
HapII CCGG 2 cut(s) 345, 1504
HincII GTYRAC 1 cut(s) 804
HindII GTYRAC 1 cut(s) 804
HinfI GANTC 4 cut(s) 443, 639, 1120, 1427
HpaII CCGG 2 cut(s) 345, 1504
HphI GGTGA 3 cut(s) 1016, 1046, 1672
Hpy166II GTNNAC 7 cut(s) 532, 648, 674, 804, 922, 1642, 1663
Hpy188I TCNGA 9 cut(s) 10, 430, 543, 603, 731, 863, 1426, 1467, 1657
Hpy188III TCNNGA 5 cut(s) 1117, 1144, 1436, 1556, 1577
Hpy8I GTNNAC 7 cut(s) 532, 648, 674, 804, 922, 1642, 1663
Hpy99I CGWCG 1 cut(s) 905
HpyAV CCTTC 1 cut(s) 5
HpyCH4IV ACGT 4 cut(s) 924, 1181, 1386, 1676
HpyCH4V TGCA 9 cut(s) 31, 149, 249, 266, 285, 523, 928, 1151, 1272
HpyF10VI GCNNNNNNNGC 4 cut(s) 339, 934, 1019, 1348
HpyF3I CTNAG 6 cut(s) 429, 553, 600, 745, 1246, 1635
HpySE526I ACGT 4 cut(s) 924, 1181, 1386, 1676
KspI CCGCGG 1 cut(s) 1109
Kzo9I GATC 5 cut(s) 105, 328, 433, 525, 628
LguI GCTCTTC 2 cut(s) 553, 1341
LmnI GCTCC 2 cut(s) 29, 339
Lsp1109I GCAGC 1 cut(s) 1599
LweI GCATC 1 cut(s) 359
MaeI CTAG 5 cut(s) 383, 408, 980, 1301, 1611
MaeII ACGT 4 cut(s) 924, 1181, 1386, 1676
MaeIII GTNAC 8 cut(s) 220, 1034, 1085, 1177, 1182, 1274, 1303, 1433
MalI GATC 5 cut(s) 107, 330, 435, 527, 630
MboI GATC 5 cut(s) 105, 328, 433, 525, 628
MhlI GDGCHC 3 cut(s) 52, 102, 344
MlsI TGGCCA 1 cut(s) 1013
MluNI TGGCCA 1 cut(s) 1013
MlyI GAGTC 1 cut(s) 1114
Mox20I TGGCCA 1 cut(s) 1013
Mph1103I ATGCAT 1 cut(s) 1153
MscI TGGCCA 1 cut(s) 1013
MseI TTAA 1 cut(s) 717
MslI CAYNNNNRTG 1 cut(s) 229
Msp20I TGGCCA 1 cut(s) 1013
MspA1I CMGCKG 1 cut(s) 1108
MspI CCGG 2 cut(s) 345, 1504
MspR9I CCNGG 2 cut(s) 346, 449
Mva1269I GAATGC 2 cut(s) 815, 1153
MvaI CCWGG 1 cut(s) 449
MvnI CGCG 1 cut(s) 1108
MwoI GCNNNNNNNGC 4 cut(s) 339, 934, 1019, 1348
NciI CCSGG 1 cut(s) 346
NcoI CCATGG 1 cut(s) 778
NdeII GATC 5 cut(s) 105, 328, 433, 525, 628
NlaIV GGNNCC 2 cut(s) 991, 1508
NmuCI GTSAC 5 cut(s) 1034, 1085, 1177, 1303, 1433
NsiI ATGCAT 1 cut(s) 1153
NspI RCATGY 2 cut(s) 337, 1382
PaeI GCATGC 1 cut(s) 1382
PagI TCATGA 1 cut(s) 1143
PaqCI CACCTGC 1 cut(s) 928
PasI CCCWGGG 1 cut(s) 448
PciSI GCTCTTC 2 cut(s) 553, 1341
PctI GAATGC 2 cut(s) 815, 1153
PfeI GAWTC 3 cut(s) 443, 639, 1427
PkrI GCNGC 1 cut(s) 1589
Ple19I CGATCG 1 cut(s) 331
PleI GAGTC 1 cut(s) 1114
PpsI GAGTC 1 cut(s) 1114
PsiI TTATAA 2 cut(s) 1235, 1367
Psp6I CCWGG 1 cut(s) 447
PspEI GGTNACC 1 cut(s) 1034
PspGI CCWGG 1 cut(s) 447
PspN4I GGNNCC 2 cut(s) 991, 1508
PsrI GAACNNNNNNTAC 2 cut(s) 394, 426
PstNI CAGNNNCTG 1 cut(s) 1430
PvuI CGATCG 1 cut(s) 331
RsaI GTAC 8 cut(s) 403, 533, 691, 833, 1128, 1333, 1621, 1641
RsaNI GTAC 8 cut(s) 402, 532, 690, 832, 1127, 1332, 1620, 1640
RseI CAYNNNNRTG 1 cut(s) 229
SacII CCGCGG 1 cut(s) 1109
SapI GCTCTTC 2 cut(s) 553, 1341
SaqAI TTAA 1 cut(s) 717
SatI GCNGC 1 cut(s) 1588
Sau3AI GATC 5 cut(s) 105, 328, 433, 525, 628
ScaI AGTACT 2 cut(s) 403, 1621
SchI GAGTC 1 cut(s) 1114
ScrFI CCNGG 2 cut(s) 346, 449
SduI GDGCHC 3 cut(s) 52, 102, 344
SfaNI GCATC 1 cut(s) 359
SfcI CTRYAG 1 cut(s) 1452
Sfr303I CCGCGG 1 cut(s) 1109
SgrBI CCGCGG 1 cut(s) 1109
SmiMI CAYNNNNRTG 1 cut(s) 229
SpeI ACTAGT 1 cut(s) 407
SphI GCATGC 1 cut(s) 1382
SsiI CCGC 4 cut(s) 1106, 1108, 1250, 1382
SspI AATATT 1 cut(s) 244
SspMI CTAG 5 cut(s) 383, 408, 980, 1301, 1611
StyD4I CCNGG 2 cut(s) 344, 447
StyI CCWWGG 1 cut(s) 778
TaiI ACGT 4 cut(s) 927, 1184, 1389, 1679
TaqI TCGA 4 cut(s) 156, 327, 1545, 1566
TatI WGTACW 6 cut(s) 401, 531, 689, 831, 1331, 1619
TfiI GAWTC 3 cut(s) 443, 639, 1427
Tru1I TTAA 1 cut(s) 717
Tru9I TTAA 1 cut(s) 717
TscAI CASTG 6 cut(s) 186, 443, 541, 1143, 1344, 1379
TseFI GTSAC 5 cut(s) 1034, 1085, 1177, 1303, 1433
TseI GCWGC 1 cut(s) 1587
Tsp45I GTSAC 5 cut(s) 1034, 1085, 1177, 1303, 1433
TspRI CASTG 6 cut(s) 186, 443, 541, 1143, 1344, 1379
XapI RAATTY 4 cut(s) 682, 1321, 1477, 1650
XceI RCATGY 2 cut(s) 337, 1382
XcmI CCANNNNNNNNNTGG 2 cut(s) 49, 1000
XmiI GTMKAC 2 cut(s) 673, 921
XspI CTAG 5 cut(s) 383, 408, 980, 1301, 1611
ZrmI AGTACT 2 cut(s) 403, 1621
Zsp2I ATGCAT 1 cut(s) 1153
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.