MD04G1101500.v1.1

MLO-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0002520
Physical Location & Seq
Forward (+)
18795431 .. 18797623
2193 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1101500.v1.1.491

Sequence Viewer

Length: 924 bp
ATGGAGCAGAGATGTTTTTTCAGGCAATTTTATAGTTCAGTGGCAAAAGTTGACTACATCACCATCCGACACGGCTTCATAGCGGCTCATTTGCCAAGCAGGCCAAGCTTCGATTTCAAAAAATACATACAGCGATCGTTGGAGGAAGATTTCAAGATCATGGTTGCAATCAGCCCTTTAATGTGGTTTGCGGTAGTCATTTTTATGCTCGTCGATGTTGTTATTCTCATGGCATTTGTTCTCTTACCAGGTTGGCATGTATATTTCTGGCTATCCTATGTTCCACTCTTGGCCGTTCTGGTTCTAGGAGCCAAACTTGAGTATATCGTTGCGAAAATGGCTCTTCAAATTAAAGAGCAGAACAGTGTGATCGTCGGAACACCACTTGTGCGAGTGAACGATGATCTCTTCTGGTTCAGGAAACCTAGATTTGTCTTGCTGCTGCTACATTATACTCTATTTTTGAATGCATTTGAGTTCGCTTTCTTCATTTGGGTTTCAATCCAATTCGGTTTCAAATCTTGCTACCACGAGCACACTGTGAGCATTGTTACGAGAATTTCCTTAGCGGTGACCACTCAAGTCCTCTGCAGCTACATCACTCTTCCTCTCTACGCACTTGTCACACAGATGGGTTCACAATTCAGGGGTAAGATAATGGAAGATAACATGGCAGACATTTTAAAGCAATGGCATGCTGAAGTGAGGAATAGAAGGAGAAAGCAACGGCAACTTCCGCAATCAGCAAGAACATCATTCTCCGCAGATTGGAGCTCTGTGAGGAATAGCTTTTCAGAAATGCCTTCTTATATAAGAAAGGTTTCCAAACTAGATGAACCAGCTGAAAGCAGTGTCCAGTTGTCTAACAGAGGGAATGTACCAGACGAGATAGTAGAACTAGATGGAAGCTCAAGGGGTTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0006952 GO:0008150 GO:0016020 GO:0044464 GO:0050896 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mlo

Protein Analysis

308

Amino Acids

35.7

Weight (kDa)

9.36

Isoelectric Point (pI)

39.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mlo PF03094 4 - 255 2.3e-101 Mlo family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 5 cut(s) 83, 191, 569, 737, 762
AcoI YGGCCR 1 cut(s) 291
AcsI RAATTY 1 cut(s) 558
AcuI CTGAAG 1 cut(s) 720
AdeI CACNNNGTG 1 cut(s) 541
AfaI GTAC 1 cut(s) 879
AfiI CCNNNNNNNGG 1 cut(s) 768
AgsI TTSAA 6 cut(s) 118, 154, 347, 466, 501, 517
AjnI CCWGG 1 cut(s) 247
AluBI AGCT 6 cut(s) 108, 594, 774, 789, 842, 909
AluI AGCT 6 cut(s) 108, 594, 774, 789, 842, 909
Alw21I GWGCWC 2 cut(s) 537, 776
AoxI GGCC 2 cut(s) 101, 291
ApeKI GCWGC 3 cut(s) 439, 442, 591
ApoI RAATTY 1 cut(s) 558
Asp700I GAANNNNTTC 1 cut(s) 820
AsuHPI GGTGA 2 cut(s) 52, 583
BanII GRGCYC 1 cut(s) 776
BauI CACGAG 1 cut(s) 530
Bbv12I GWGCWC 2 cut(s) 537, 776
BbvI GCAGC 3 cut(s) 426, 429, 603
BccI CCATC 3 cut(s) 71, 625, 896
BceAI ACGGC 3 cut(s) 88, 278, 743
BciT130I CCWGG 1 cut(s) 249
BfaI CTAG 4 cut(s) 305, 426, 830, 899
BfmI CTRYAG 1 cut(s) 589
BglI GCCNNNNNGGC 1 cut(s) 100
BisI GCNGC 4 cut(s) 84, 440, 443, 592
BlsI GCNGC 4 cut(s) 85, 441, 444, 593
Bme1390I CCNGG 1 cut(s) 249
BmiI GGNNCC 1 cut(s) 310
BmrFI CCNGG 1 cut(s) 249
Bpu10I CCTNAGC 1 cut(s) 565
BpuEI CTTGAG 3 cut(s) 338, 564, 895
Bsc4I CCNNNNNNNGG 1 cut(s) 768
Bse1I ACTGG 1 cut(s) 856
Bse3DI GCAATG 1 cut(s) 695
BseBI CCWGG 1 cut(s) 249
BseGI GGATG 1 cut(s) 63
BseLI CCNNNNNNNGG 1 cut(s) 768
BseMI GCAATG 1 cut(s) 695
BseNI ACTGG 1 cut(s) 856
BseXI GCAGC 3 cut(s) 426, 429, 603
Bsh1285I CGRYCG 1 cut(s) 137
BshFI GGCC 2 cut(s) 103, 293
BsiEI CGRYCG 1 cut(s) 137
BsiHKAI GWGCWC 2 cut(s) 537, 776
BslI CCNNNNNNNGG 1 cut(s) 768
BsmI GAATGC 1 cut(s) 472
BsnI GGCC 2 cut(s) 103, 293
Bsp1286I GDGCHC 2 cut(s) 537, 776
Bsp143I GATC 4 cut(s) 134, 156, 369, 403
BspACI CCGC 5 cut(s) 83, 191, 569, 737, 762
BspANI GGCC 2 cut(s) 103, 293
BspLI GGNNCC 1 cut(s) 310
BspMAI CTGCAG 1 cut(s) 593
BspQI GCTCTTC 1 cut(s) 348
BsrDI GCAATG 1 cut(s) 695
BsrI ACTGG 1 cut(s) 856
BssMI GATC 4 cut(s) 134, 156, 369, 403
BssSI CACGAG 1 cut(s) 530
Bst2BI CACGAG 1 cut(s) 530
Bst2UI CCWGG 1 cut(s) 249
Bst4CI ACNGT 2 cut(s) 365, 541
Bst6I CTCTTC 3 cut(s) 348, 413, 609
BstC8I GCNNGC 2 cut(s) 101, 696
BstDEI CTNAG 1 cut(s) 565
BstEII GGTNACC 1 cut(s) 571
BstF5I GGATG 1 cut(s) 63
BstKTI GATC 4 cut(s) 137, 159, 372, 406
BstMBI GATC 4 cut(s) 134, 156, 369, 403
BstMCI CGRYCG 1 cut(s) 137
BstMWI GCNNNNNNNGC 4 cut(s) 100, 105, 338, 736
BstNI CCWGG 1 cut(s) 249
BstNSI RCATGY 2 cut(s) 260, 698
BstPI GGTNACC 1 cut(s) 571
BstSCI CCNGG 1 cut(s) 247
BstSFI CTRYAG 1 cut(s) 589
BstV1I GCAGC 3 cut(s) 426, 429, 603
BsuRI GGCC 2 cut(s) 103, 293
BtsCI GGATG 1 cut(s) 63
BtsI GCAGTG 1 cut(s) 856
BtsIMutI CAGTG 4 cut(s) 45, 370, 537, 856
Cac8I GCNNGC 2 cut(s) 101, 696
CsiI ACCWGGT 1 cut(s) 247
Csp6I GTAC 1 cut(s) 878
CviAII CATG 5 cut(s) 160, 229, 257, 670, 695
CviQI GTAC 1 cut(s) 878
DdeI CTNAG 1 cut(s) 565
DpnI GATC 4 cut(s) 136, 158, 371, 405
DpnII GATC 4 cut(s) 134, 156, 369, 403
DraI TTTAAA 1 cut(s) 684
DraIII CACNNNGTG 1 cut(s) 541
EaeI YGGCCR 1 cut(s) 291
Eam1104I CTCTTC 3 cut(s) 348, 413, 609
EarI CTCTTC 3 cut(s) 348, 413, 609
Ecl136II GAGCTC 1 cut(s) 774
Eco24I GRGCYC 1 cut(s) 776
Eco53kI GAGCTC 1 cut(s) 774
Eco57I CTGAAG 1 cut(s) 720
Eco91I GGTNACC 1 cut(s) 571
EcoICRI GAGCTC 1 cut(s) 774
EcoO65I GGTNACC 1 cut(s) 571
EcoRII CCWGG 1 cut(s) 247
EcoT22I ATGCAT 1 cut(s) 472
EcoT38I GRGCYC 1 cut(s) 776
FaeI CATG 5 cut(s) 163, 232, 260, 673, 698
FatI CATG 5 cut(s) 159, 228, 256, 669, 694
Fnu4HI GCNGC 4 cut(s) 84, 440, 443, 592
FokI GGATG 1 cut(s) 50
FriOI GRGCYC 1 cut(s) 776
Fsp4HI GCNGC 4 cut(s) 84, 440, 443, 592
FspBI CTAG 4 cut(s) 305, 426, 830, 899
GluI GCNGC 4 cut(s) 84, 440, 443, 592
HaeIII GGCC 2 cut(s) 103, 293
Hin1II CATG 5 cut(s) 163, 232, 260, 673, 698
HincII GTYRAC 1 cut(s) 52
HindII GTYRAC 1 cut(s) 52
HindIII AAGCTT 1 cut(s) 106
HphI GGTGA 2 cut(s) 52, 583
Hpy166II GTNNAC 3 cut(s) 52, 397, 638
Hpy188I TCNGA 3 cut(s) 68, 377, 796
Hpy188III TCNNGA 2 cut(s) 154, 418
Hpy8I GTNNAC 3 cut(s) 52, 397, 638
Hpy99I CGWCG 2 cut(s) 215, 377
HpyAV CCTTC 2 cut(s) 708, 813
HpyCH4III ACNGT 2 cut(s) 365, 541
HpyCH4V TGCA 3 cut(s) 167, 470, 591
HpyF10VI GCNNNNNNNGC 4 cut(s) 100, 105, 338, 736
HpyF3I CTNAG 1 cut(s) 565
Hsp92II CATG 5 cut(s) 163, 232, 260, 673, 698
Kzo9I GATC 4 cut(s) 134, 156, 369, 403
LguI GCTCTTC 1 cut(s) 348
LmnI GCTCC 3 cut(s) 4, 308, 771
Lsp1109I GCAGC 3 cut(s) 426, 429, 603
MabI ACCWGGT 1 cut(s) 247
MaeI CTAG 4 cut(s) 305, 426, 830, 899
MaeIII GTNAC 3 cut(s) 550, 571, 622
MalI GATC 4 cut(s) 136, 158, 371, 405
MboI GATC 4 cut(s) 134, 156, 369, 403
MboII GAAGA 6 cut(s) 158, 335, 400, 478, 596, 674
MhlI GDGCHC 2 cut(s) 537, 776
MluCI AATT 5 cut(s) 26, 348, 506, 558, 641
MmeI TCCRAC 3 cut(s) 91, 120, 355
MnlI CCTC 6 cut(s) 136, 596, 618, 699, 774, 863
Mph1103I ATGCAT 1 cut(s) 472
MroXI GAANNNNTTC 1 cut(s) 820
MseI TTAA 3 cut(s) 179, 351, 683
MslI CAYNNNNRTG 2 cut(s) 203, 629
MspA1I CMGCKG 1 cut(s) 842
MspR9I CCNGG 1 cut(s) 249
Mva1269I GAATGC 1 cut(s) 472
MvaI CCWGG 1 cut(s) 249
MwoI GCNNNNNNNGC 4 cut(s) 100, 105, 338, 736
NdeII GATC 4 cut(s) 134, 156, 369, 403
NlaIII CATG 5 cut(s) 163, 232, 260, 673, 698
NlaIV GGNNCC 1 cut(s) 310
NmuCI GTSAC 2 cut(s) 571, 622
NsiI ATGCAT 1 cut(s) 472
NspI RCATGY 2 cut(s) 260, 698
PaeI GCATGC 1 cut(s) 698
PciSI GCTCTTC 1 cut(s) 348
PctI GAATGC 1 cut(s) 472
PdmI GAANNNNTTC 1 cut(s) 820
PkrI GCNGC 4 cut(s) 85, 441, 444, 593
Ple19I CGATCG 1 cut(s) 137
Psp124BI GAGCTC 1 cut(s) 776
Psp6I CCWGG 1 cut(s) 247
PspEI GGTNACC 1 cut(s) 571
PspGI CCWGG 1 cut(s) 247
PspN4I GGNNCC 1 cut(s) 310
PstI CTGCAG 1 cut(s) 593
PvuI CGATCG 1 cut(s) 137
PvuII CAGCTG 1 cut(s) 842
RsaI GTAC 1 cut(s) 879
RsaNI GTAC 1 cut(s) 878
RseI CAYNNNNRTG 2 cut(s) 203, 629
SacI GAGCTC 1 cut(s) 776
SapI GCTCTTC 1 cut(s) 348
SaqAI TTAA 3 cut(s) 179, 351, 683
SatI GCNGC 4 cut(s) 84, 440, 443, 592
Sau3AI GATC 4 cut(s) 134, 156, 369, 403
ScrFI CCNGG 1 cut(s) 249
SduI GDGCHC 2 cut(s) 537, 776
SetI ASST 9 cut(s) 110, 253, 427, 596, 776, 791, 822, 844, 911
SexAI ACCWGGT 1 cut(s) 247
SfcI CTRYAG 1 cut(s) 589
SmiMI CAYNNNNRTG 2 cut(s) 203, 629
SmlI CTYRAG 3 cut(s) 317, 579, 910
SmoI CTYRAG 3 cut(s) 317, 579, 910
SphI GCATGC 1 cut(s) 698
Sse9I AATT 5 cut(s) 26, 348, 506, 558, 641
SsiI CCGC 5 cut(s) 83, 191, 569, 737, 762
SspMI CTAG 4 cut(s) 305, 426, 830, 899
SstI GAGCTC 1 cut(s) 776
StyD4I CCNGG 1 cut(s) 247
TaaI ACNGT 2 cut(s) 365, 541
TaqI TCGA 2 cut(s) 111, 213
TasI AATT 5 cut(s) 26, 348, 506, 558, 641
TauI GCSGC 1 cut(s) 86
Tru1I TTAA 3 cut(s) 179, 351, 683
Tru9I TTAA 3 cut(s) 179, 351, 683
TscAI CASTG 4 cut(s) 45, 370, 544, 856
TseFI GTSAC 2 cut(s) 571, 622
TseI GCWGC 3 cut(s) 439, 442, 591
Tsp45I GTSAC 2 cut(s) 571, 622
TspDTI ATGAA 3 cut(s) 67, 478, 849
TspRI CASTG 4 cut(s) 45, 370, 544, 856
XapI RAATTY 1 cut(s) 558
XceI RCATGY 2 cut(s) 260, 698
XmnI GAANNNNTTC 1 cut(s) 820
XspI CTAG 4 cut(s) 305, 426, 830, 899
Zsp2I ATGCAT 1 cut(s) 472
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.