Rh5AG391300

MLO-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0002520
Physical Location & Seq
Forward (+)
67796060 .. 67801699
5640 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG391300.1

Sequence Viewer

Length: 1233 bp
ATGACTTCCGAAGGAACAAGTCGCTCCCTGCAAGACACACCCAGTTGGGCTCTGGCAACAGTTTGCTTCGTCTTCATCTTTCTCTCTCTTTTCATAGAGCACTTGATCCATCTCCTTTCCCAATTTCTCAAAAAGCATAGAAAGACTGCATTGTTCGAGGCCGTAGAGAAGCTCAAATCAGTGCTTATTTTTCTGGGTTTCATGTCCCTAATACTGACAGTAACACAGAGATGGATAGCCAATATTTGCATACCCACAGAAGTTGCATATACTATGCTTCCTTGCAGAAAATCAGTATCAACTAAAACAACCAAAGCAGTTGGTTTCGATCGCATGTGGAGCACCGGGGCATCTACAGCTTTTGAAGTAATACAAAGAAGACTAGCAGAAACAGAAGATAGTACTACTAGTTCCACTTCTACCACTTCTGTGGATGTGGATCAGTGCGAATCCCAGGGGAAGACCTCATTTATATCTCAAGGGGGACTAAATCAGCTCAACAACTTCATCTTTGTTCTGGCAATTATGCAGATCGTGTACAGTGTTCTGACAATGACCTTAGGAAGAGCCAAGATGAAGCGTTGGGAAGCGTGGGAAAGAGAAACTCAGACGGTGGAATACCAAGTCGCAAATGATCCTAACCGATTCAGGTTTACAAGGCAGACGACATTTGCTCGTAGACATATGAATTCTTGTACAGGAACACCTATTCAACTGTGGATTAAGTGCTTCTTCAGACAATTCTTCCACTCAGTTGAAAAAATTGATTATCTCACATTACGCCATGGCTTCATTTCTACTCATTTGTCAACAAACAACGCATTCAATTTCCAGCAGTACATAGAACGGTCGCTGGAGGATGATTTCAGAGAAGTAGTTGGCATAAGCCCTCTCATGTGGTTTATCGTCGTAATCTTCATTCTCGTAGACGTGCATGGTTGGCAGGTGTATCTCTGGGTGTCATTTGTGCCACTAATCATTGTGCTAGTTCTTGGAACCAAACTGGAAGTGGTGGTAGCCAGAATGGCTCACCAACTCGGTAACCAGCATAATGTAATCAAAGGAACTCCTCTGGTTCAACCAAAGGACAGTCACTTCTGGTTCAATAGACCACGGTTCGTCTTGACTCTCGTACATCTCACACTGTTCATGAATGCATTTGAACTTGCTTTCTTCATTTGGGTCACGGTACGTATGTCCTTATTTCAACTTAGGACCAAGTTTCAAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0006952 GO:0008150 GO:0016020 GO:0044464 GO:0050896 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mlo

Protein Analysis

410

Amino Acids

47.54

Weight (kDa)

9.55

Isoelectric Point (pI)

31.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mlo PF03094 8 - 400 6.3e-157 Mlo family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 934
Acc36I ACCTGC 1 cut(s) 934
AccI GTMKAC 2 cut(s) 679, 927
AclWI GGATC 3 cut(s) 100, 447, 629
AcsI RAATTY 1 cut(s) 688
AcuI CTGAAG 1 cut(s) 718
AfaI GTAC 6 cut(s) 403, 539, 697, 839, 1134, 1191
AgsI TTSAA 9 cut(s) 365, 713, 758, 826, 1079, 1105, 1163, 1208, 1226
AhlI ACTAGT 1 cut(s) 407
AjiI CACGTC 1 cut(s) 931
AjnI CCWGG 1 cut(s) 453
AleI CACNNNNGTG 1 cut(s) 428
AluBI AGCT 3 cut(s) 172, 359, 496
AluI AGCT 3 cut(s) 172, 359, 496
Alw21I GWGCWC 2 cut(s) 102, 344
AlwI GGATC 3 cut(s) 100, 447, 629
AoxI GGCC 1 cut(s) 159
ApoI RAATTY 1 cut(s) 688
AspS9I GGNCC 1 cut(s) 1215
AsuC2I CCSGG 1 cut(s) 346
AsuHPI GGTGA 1 cut(s) 1022
AvaII GGWCC 1 cut(s) 1215
AxyI CCTNAGG 1 cut(s) 559
BaeI ACNNNNGTAYC 2 cut(s) 1181, 1214
BanII GRGCYC 1 cut(s) 52
BbsI GAAGAC 3 cut(s) 64, 385, 467
Bbv12I GWGCWC 2 cut(s) 102, 344
BccI CCATC 2 cut(s) 117, 225
BceAI ACGGC 1 cut(s) 146
BciT130I CCWGG 1 cut(s) 455
BcnI CCSGG 1 cut(s) 346
BcuI ACTAGT 1 cut(s) 407
BfaI CTAG 3 cut(s) 383, 408, 986
BfmI CTRYAG 1 cut(s) 354
BfuAI ACCTGC 1 cut(s) 934
BglI GCCNNNNNGGC 1 cut(s) 1025
BmcAI AGTACT 1 cut(s) 403
Bme1390I CCNGG 2 cut(s) 346, 455
Bme18I GGWCC 1 cut(s) 1215
BmgBI CACGTC 1 cut(s) 931
BmgT120I GGNCC 1 cut(s) 1215
BmiI GGNNCC 1 cut(s) 997
BmrFI CCNGG 2 cut(s) 346, 455
BmrI ACTGGG 1 cut(s) 36
BmsI GCATC 1 cut(s) 359
BmuI ACTGGG 1 cut(s) 36
BpiI GAAGAC 3 cut(s) 64, 385, 467
BpmI CTGGAG 1 cut(s) 875
BpuEI CTTGAG 1 cut(s) 462
BpuMI CCSGG 1 cut(s) 346
BsaAI YACGTR 1 cut(s) 1193
BsaBI GATNNNNATC 1 cut(s) 438
BsaJI CCNNGG 5 cut(s) 345, 453, 454, 784, 1112
Bse1I ACTGG 2 cut(s) 42, 1008
Bse21I CCTNAGG 1 cut(s) 559
Bse8I GATNNNNATC 1 cut(s) 438
BseBI CCWGG 1 cut(s) 455
BseDI CCNNGG 5 cut(s) 345, 453, 454, 784, 1112
BseGI GGATG 2 cut(s) 439, 865
BseJI GATNNNNATC 1 cut(s) 438
BseMII CTCAG 2 cut(s) 620, 765
BseNI ACTGG 2 cut(s) 42, 1008
BseRI GAGGAG 1 cut(s) 1059
Bsh1285I CGRYCG 2 cut(s) 331, 851
BshFI GGCC 1 cut(s) 161
BsiEI CGRYCG 2 cut(s) 331, 851
BsiHKAI GWGCWC 2 cut(s) 102, 344
BsiSI CCGG 1 cut(s) 345
BslFI GGGAC 2 cut(s) 190, 498
BsmFI GGGAC 2 cut(s) 190, 498
BsmI GAATGC 2 cut(s) 821, 1159
BsnI GGCC 1 cut(s) 161
Bsp1286I GDGCHC 3 cut(s) 52, 102, 344
Bsp1407I TGTACA 2 cut(s) 537, 695
Bsp143I GATC 5 cut(s) 105, 328, 439, 531, 634
Bsp19I CCATGG 1 cut(s) 784
BspANI GGCC 1 cut(s) 161
BspCNI CTCAG 2 cut(s) 619, 764
BspHI TCATGA 1 cut(s) 1149
BspLI GGNNCC 1 cut(s) 997
BspMI ACCTGC 1 cut(s) 934
BspPI GGATC 3 cut(s) 100, 447, 629
BspQI GCTCTTC 1 cut(s) 559
BsrGI TGTACA 2 cut(s) 537, 695
BsrI ACTGG 2 cut(s) 42, 1008
BssECI CCNNGG 5 cut(s) 345, 453, 454, 784, 1112
BssMI GATC 5 cut(s) 105, 328, 439, 531, 634
BssT1I CCWWGG 1 cut(s) 784
Bst2UI CCWGG 1 cut(s) 455
Bst6I CTCTTC 1 cut(s) 559
BstAUI TGTACA 2 cut(s) 537, 695
BstBAI YACGTR 1 cut(s) 1193
BstDEI CTNAG 4 cut(s) 559, 606, 751, 1211
BstDSI CCRYGG 2 cut(s) 784, 1112
BstEII GGTNACC 1 cut(s) 1040
BstF5I GGATG 2 cut(s) 439, 865
BstKTI GATC 5 cut(s) 108, 331, 442, 534, 637
BstMBI GATC 5 cut(s) 105, 328, 439, 531, 634
BstMCI CGRYCG 2 cut(s) 331, 851
BstMWI GCNNNNNNNGC 4 cut(s) 339, 356, 940, 1025
BstNI CCWGG 1 cut(s) 455
BstNSI RCATGY 1 cut(s) 337
BstPI GGTNACC 1 cut(s) 1040
BstSCI CCNGG 2 cut(s) 344, 453
BstSFI CTRYAG 1 cut(s) 354
BstSNI TACGTA 1 cut(s) 1193
BstV2I GAAGAC 3 cut(s) 64, 385, 467
BstXI CCANNNNNNTGG 1 cut(s) 430
Bsu36I CCTNAGG 1 cut(s) 559
BsuRI GGCC 1 cut(s) 161
BtgI CCRYGG 2 cut(s) 784, 1112
BtrI CACGTC 1 cut(s) 931
BtsCI GGATG 2 cut(s) 439, 865
BtsIMutI CAGTG 4 cut(s) 186, 449, 547, 1142
BveI ACCTGC 1 cut(s) 934
CciI TCATGA 1 cut(s) 1149
Cfr13I GGNCC 1 cut(s) 1215
Csp6I GTAC 6 cut(s) 402, 538, 696, 838, 1133, 1190
CspCI CAANNNNNGTGG 2 cut(s) 244, 279
CviAII CATG 6 cut(s) 202, 334, 785, 895, 935, 1150
CviQI GTAC 6 cut(s) 402, 538, 696, 838, 1133, 1190
DdeI CTNAG 4 cut(s) 559, 606, 751, 1211
DpnI GATC 5 cut(s) 107, 330, 441, 533, 636
DpnII GATC 5 cut(s) 105, 328, 439, 531, 634
Eam1104I CTCTTC 1 cut(s) 559
EarI CTCTTC 1 cut(s) 559
Eco105I TACGTA 1 cut(s) 1193
Eco130I CCWWGG 1 cut(s) 784
Eco24I GRGCYC 1 cut(s) 52
Eco47I GGWCC 1 cut(s) 1215
Eco57I CTGAAG 1 cut(s) 718
Eco81I CCTNAGG 1 cut(s) 559
Eco91I GGTNACC 1 cut(s) 1040
EcoO65I GGTNACC 1 cut(s) 1040
EcoRI GAATTC 1 cut(s) 688
EcoRII CCWGG 1 cut(s) 453
EcoT14I CCWWGG 1 cut(s) 784
EcoT22I ATGCAT 1 cut(s) 1159
EcoT38I GRGCYC 1 cut(s) 52
ErhI CCWWGG 1 cut(s) 784
FaeI CATG 6 cut(s) 205, 337, 788, 898, 938, 1153
FalI AAGNNNNNCTT 2 cut(s) 716, 748
FaqI GGGAC 2 cut(s) 190, 498
FatI CATG 6 cut(s) 201, 333, 784, 894, 934, 1149
FauNDI CATATG 1 cut(s) 684
FblI GTMKAC 2 cut(s) 679, 927
FokI GGATG 2 cut(s) 446, 872
FriOI GRGCYC 1 cut(s) 52
FspBI CTAG 3 cut(s) 383, 408, 986
GsuI CTGGAG 1 cut(s) 875
HaeIII GGCC 1 cut(s) 161
HapII CCGG 1 cut(s) 345
Hin1II CATG 6 cut(s) 205, 337, 788, 898, 938, 1153
HincII GTYRAC 1 cut(s) 810
HindII GTYRAC 1 cut(s) 810
HinfI GANTC 3 cut(s) 449, 645, 1126
HpaII CCGG 1 cut(s) 345
HphI GGTGA 1 cut(s) 1022
Hpy166II GTNNAC 5 cut(s) 538, 654, 680, 810, 928
Hpy188I TCNGA 5 cut(s) 10, 549, 609, 737, 869
Hpy188III TCNNGA 2 cut(s) 1123, 1150
Hpy8I GTNNAC 5 cut(s) 538, 654, 680, 810, 928
Hpy99I CGWCG 1 cut(s) 911
HpyAV CCTTC 1 cut(s) 5
HpyCH4IV ACGT 2 cut(s) 930, 1192
HpyCH4V TGCA 8 cut(s) 31, 149, 249, 266, 285, 529, 934, 1157
HpyF10VI GCNNNNNNNGC 4 cut(s) 339, 356, 940, 1025
HpyF3I CTNAG 4 cut(s) 559, 606, 751, 1211
HpySE526I ACGT 2 cut(s) 930, 1192
Hsp92II CATG 6 cut(s) 205, 337, 788, 898, 938, 1153
Kzo9I GATC 5 cut(s) 105, 328, 439, 531, 634
LguI GCTCTTC 1 cut(s) 559
LmnI GCTCC 2 cut(s) 29, 339
LweI GCATC 1 cut(s) 359
MaeI CTAG 3 cut(s) 383, 408, 986
MaeII ACGT 2 cut(s) 930, 1192
MaeIII GTNAC 4 cut(s) 220, 1040, 1091, 1183
MalI GATC 5 cut(s) 107, 330, 441, 533, 636
MboI GATC 5 cut(s) 105, 328, 439, 531, 634
MboII GAAGA 9 cut(s) 64, 390, 407, 472, 576, 724, 736, 907, 1165
MhlI GDGCHC 3 cut(s) 52, 102, 344
MluCI AATT 6 cut(s) 122, 522, 688, 740, 762, 826
MlyI GAGTC 1 cut(s) 1120
MnlI CCTC 5 cut(s) 151, 475, 850, 900, 1080
Mph1103I ATGCAT 1 cut(s) 1159
MseI TTAA 1 cut(s) 723
MslI CAYNNNNRTG 2 cut(s) 229, 428
MspI CCGG 1 cut(s) 345
MspR9I CCNGG 2 cut(s) 346, 455
Mva1269I GAATGC 2 cut(s) 821, 1159
MvaI CCWGG 1 cut(s) 455
MwoI GCNNNNNNNGC 4 cut(s) 339, 356, 940, 1025
NciI CCSGG 1 cut(s) 346
NcoI CCATGG 1 cut(s) 784
NdeI CATATG 1 cut(s) 684
NdeII GATC 5 cut(s) 105, 328, 439, 531, 634
NlaIII CATG 6 cut(s) 205, 337, 788, 898, 938, 1153
NlaIV GGNNCC 1 cut(s) 997
NmuCI GTSAC 2 cut(s) 1091, 1183
NsiI ATGCAT 1 cut(s) 1159
NspI RCATGY 1 cut(s) 337
OliI CACNNNNGTG 1 cut(s) 428
PagI TCATGA 1 cut(s) 1149
PaqCI CACCTGC 1 cut(s) 934
PasI CCCWGGG 1 cut(s) 454
PciSI GCTCTTC 1 cut(s) 559
PctI GAATGC 2 cut(s) 821, 1159
PfeI GAWTC 2 cut(s) 449, 645
Ple19I CGATCG 1 cut(s) 331
PleI GAGTC 1 cut(s) 1120
PpsI GAGTC 1 cut(s) 1120
Ppu21I YACGTR 1 cut(s) 1193
Psp6I CCWGG 1 cut(s) 453
PspEI GGTNACC 1 cut(s) 1040
PspGI CCWGG 1 cut(s) 453
PspN4I GGNNCC 1 cut(s) 997
PspPI GGNCC 1 cut(s) 1215
PsrI GAACNNNNNNTAC 2 cut(s) 394, 426
PvuI CGATCG 1 cut(s) 331
RsaI GTAC 6 cut(s) 403, 539, 697, 839, 1134, 1191
RsaNI GTAC 6 cut(s) 402, 538, 696, 838, 1133, 1190
RseI CAYNNNNRTG 2 cut(s) 229, 428
SapI GCTCTTC 1 cut(s) 559
SaqAI TTAA 1 cut(s) 723
Sau3AI GATC 5 cut(s) 105, 328, 439, 531, 634
Sau96I GGNCC 1 cut(s) 1215
ScaI AGTACT 1 cut(s) 403
SchI GAGTC 1 cut(s) 1120
ScrFI CCNGG 2 cut(s) 346, 455
SduI GDGCHC 3 cut(s) 52, 102, 344
SfaNI GCATC 1 cut(s) 359
SfcI CTRYAG 1 cut(s) 354
SinI GGWCC 1 cut(s) 1215
SmiMI CAYNNNNRTG 2 cut(s) 229, 428
SmlI CTYRAG 1 cut(s) 477
SmoI CTYRAG 1 cut(s) 477
SnaBI TACGTA 1 cut(s) 1193
SpeI ACTAGT 1 cut(s) 407
Sse9I AATT 6 cut(s) 122, 522, 688, 740, 762, 826
SspI AATATT 1 cut(s) 244
SspMI CTAG 3 cut(s) 383, 408, 986
StyD4I CCNGG 2 cut(s) 344, 453
StyI CCWWGG 1 cut(s) 784
TaiI ACGT 2 cut(s) 933, 1195
TaqI TCGA 2 cut(s) 156, 327
TasI AATT 6 cut(s) 122, 522, 688, 740, 762, 826
TatI WGTACW 4 cut(s) 401, 537, 695, 837
TfiI GAWTC 2 cut(s) 449, 645
Tru1I TTAA 1 cut(s) 723
Tru9I TTAA 1 cut(s) 723
TscAI CASTG 4 cut(s) 186, 449, 547, 1149
TseFI GTSAC 2 cut(s) 1091, 1183
Tsp45I GTSAC 2 cut(s) 1091, 1183
TspRI CASTG 4 cut(s) 186, 449, 547, 1149
VpaK11BI GGWCC 1 cut(s) 1215
XapI RAATTY 1 cut(s) 688
XceI RCATGY 1 cut(s) 337
XcmI CCANNNNNNNNNTGG 2 cut(s) 49, 1006
XmiI GTMKAC 2 cut(s) 679, 927
XspI CTAG 3 cut(s) 383, 408, 986
ZrmI AGTACT 1 cut(s) 403
Zsp2I ATGCAT 1 cut(s) 1159
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.