RLG00000035337

MLO-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0002520
Physical Location & Seq
Forward (+)
65266573 .. 65272740
6168 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000035337

Sequence Viewer

Length: 1671 bp
ATGGCTTCCGAAGGAACGAGTCGCTCCCTGCAAGAAACACCCAGTTGGGCTCTGGCAACAGTTTGCTTCGTCTTTATCTTTCTCTCTCTCTTCATAGAGCACTTGATCCATCTCCTTTCCCAATTTCTCAAAAAGCATAGAAAGACTGCATTGTTCGAGGCCGTAGAGAAGCTAAAATCAGTGCTTATTTTTCTGGGTTTCATGTCCCTAATACTGACAGTAACACAGAGATGGATAGCCAATATTTGCATACCCACAGAAGTTGCATATACTATGCTTCCTTGCAGAAAATCAGTATCAACTAAAACAACCAAAGCAGTTGGTTTCGATCGCATGTGGAGCACCGGGGCATCTACAGCTTTTGAAGTAATACAAAGAAGACTAGCAGAAACAGAAGATACTGCTAGTTCCACTTCTACCACTTCTGTGGATCAGTGCGAATCCCAGGGGAAGACCTCATTTATATCACAAATTGGACTAAATCAGCTCAACAACTTCATCTTTGTTCTGGCAGTTATGCAGATCGTGTACAGTGTTCTGACAATGGCCTTAGGAAGAGCCAAGATGAAGCGTTGGGAAGCGTGGGAAAGAGAAACTCAGACGGTGGAATACCAAGTCGCAAATGATCCTAACCGATTCAGGTTTACAAGGCAGACGACATTTGCTCGTAGACACATGAATTCTTGTACAGGAACACCTATTCAACTGTGGATTAAGTGCTTCTTCAGACAATTCTTCCACTCAGTTGAAAAAATTGATTATCTCACATTACGCCATGGCTTCATTTCTACTCATTTGTCAACAAACAACGCATTCAATTTCCAGCAGTACATAGAACGGTCGCTGGAGGATGATTTCAGAAAAGTAGTTGGCATAAGCCCTTTCATGTGGTTTATCGTCGTAATCTTCATTCTCGTAGACGTGCATGGTTGGCAGGTGTATCTCTGGGTGTCATTTGTGCCACTAATTATTGTGCTGGTTCTTGGAACCAAACTGGAAGTGGTGGTAGCCAGAATGGCTCACCAACTTGGTGACCAGCATAATGTAATCAAAGGAACTCCTCTGGTTCAACCAAAGGACAGTCACTTCTGGTTCAATAGACCGCGGTTCGTCTTGCATCTCGTACATCTCACACTGTTCATGAATGCATTTGAGCTTGCTTTCTTCATTTGGGTCACGTTGCAGTTTGGGATACACTCTTGCTACCATGAACACATAGAGATTATCATTATAAGGGTGGTCTTAGCGGTTGTCGTTCAAGTTATGTGCAGTTACATTACTCTTCCCCTCTATGCTCTAGTGACCCAGATGGGGTCAAATTTGAAGGGTACAGCAGTGCTTGAAGAGCAAACAACAAAAGTTATAAAGCAGTGGCATGCGGACGTGAAGCAAAAGAGGAAAAAGAATAGGCAAGACAATTCAGAATCTGGTCACGATTATTCCTCTACTGTAGGAAGCAGTCAGAGAACCATGATTTCCCCAGATTTCTCTTCCCACCACCGGCACCCAACTTTTGCTGAAATGTCCCAAACGGAGATTGTCGAAGATGGTCAAGAAATTGTTCAAGAACATCAAGAAAAGGGCAGCCAAAATGAAATTGAGCTAGCTAGTACTGTTTTAGCTACTGAGGTACACATAGAAATTTCGGAGGTGAACAGAAGCCAAACGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0006952 GO:0008150 GO:0016020 GO:0044464 GO:0050896 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mlo

Protein Analysis

557

Amino Acids

63.93

Weight (kDa)

8.33

Isoelectric Point (pI)

42.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mlo PF03094 8 - 486 6.4e-195 Mlo family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1232, 1364
AarI CACCTGC 1 cut(s) 925
Acc36I ACCTGC 1 cut(s) 925
AccB1I GGYRCC 1 cut(s) 1503
AccI GTMKAC 2 cut(s) 670, 918
AccII CGCG 1 cut(s) 1105
AciI CCGC 4 cut(s) 1103, 1105, 1247, 1379
AclWI GGATC 3 cut(s) 100, 438, 620
AcsI RAATTY 3 cut(s) 679, 1318, 1641
AcuI CTGAAG 1 cut(s) 709
AfaI GTAC 7 cut(s) 530, 688, 830, 1125, 1330, 1612, 1632
AfiI CCNNNNNNNGG 2 cut(s) 1311, 1500
AjiI CACGTC 2 cut(s) 922, 1384
AjnI CCWGG 1 cut(s) 444
AleI CACNNNNGTG 1 cut(s) 425
AluBI AGCT 7 cut(s) 172, 359, 487, 1156, 1603, 1607, 1622
AluI AGCT 7 cut(s) 172, 359, 487, 1156, 1603, 1607, 1622
Alw21I GWGCWC 2 cut(s) 102, 344
AlwI GGATC 3 cut(s) 100, 438, 620
AlwNI CAGNNNCTG 1 cut(s) 1427
AoxI GGCC 2 cut(s) 159, 546
ApeKI GCWGC 1 cut(s) 1584
ApoI RAATTY 3 cut(s) 679, 1318, 1641
Asp700I GAANNNNTTC 1 cut(s) 1560
AsuC2I CCSGG 1 cut(s) 346
AsuHPI GGTGA 3 cut(s) 1013, 1043, 1663
AsuNHI GCTAGC 1 cut(s) 1603
AxyI CCTNAGG 1 cut(s) 550
BaeI ACNNNNGTAYC 2 cut(s) 1320, 1353
BanI GGYRCC 1 cut(s) 1503
BanII GRGCYC 1 cut(s) 52
BbsI GAAGAC 2 cut(s) 385, 458
Bbv12I GWGCWC 2 cut(s) 102, 344
BbvI GCAGC 1 cut(s) 1596
BccI CCATC 4 cut(s) 117, 225, 1303, 1541
BceAI ACGGC 1 cut(s) 146
BciT130I CCWGG 1 cut(s) 446
BciVI GTATCC 1 cut(s) 1185
BcnI CCSGG 1 cut(s) 346
BfaI CTAG 5 cut(s) 383, 405, 1298, 1604, 1608
BfmI CTRYAG 2 cut(s) 354, 1449
BfuAI ACCTGC 1 cut(s) 925
BfuI GTATCC 1 cut(s) 1185
BglI GCCNNNNNGGC 1 cut(s) 1016
BisI GCNGC 1 cut(s) 1585
BlsI GCNGC 1 cut(s) 1586
BmcAI AGTACT 1 cut(s) 1612
Bme1390I CCNGG 2 cut(s) 346, 446
BmgBI CACGTC 2 cut(s) 922, 1384
BmiI GGNNCC 2 cut(s) 988, 1505
BmrFI CCNGG 2 cut(s) 346, 446
BmrI ACTGGG 1 cut(s) 36
BmsI GCATC 2 cut(s) 359, 1126
BmtI GCTAGC 1 cut(s) 1607
BmuI ACTGGG 1 cut(s) 36
BpiI GAAGAC 2 cut(s) 385, 458
BpmI CTGGAG 1 cut(s) 866
BpuMI CCSGG 1 cut(s) 346
BsaJI CCNNGG 5 cut(s) 345, 444, 445, 775, 1103
Bsc4I CCNNNNNNNGG 2 cut(s) 1311, 1500
Bse118I RCCGGY 1 cut(s) 1500
Bse1I ACTGG 2 cut(s) 42, 999
Bse21I CCTNAGG 1 cut(s) 550
BseBI CCWGG 1 cut(s) 446
BseDI CCNNGG 5 cut(s) 345, 444, 445, 775, 1103
BseGI GGATG 1 cut(s) 856
BseLI CCNNNNNNNGG 2 cut(s) 1311, 1500
BseMII CTCAG 3 cut(s) 611, 756, 1617
BseNI ACTGG 2 cut(s) 42, 999
BseRI GAGGAG 1 cut(s) 1050
BseXI GCAGC 1 cut(s) 1596
BsgI GTGCAG 1 cut(s) 1288
Bsh1236I CGCG 1 cut(s) 1105
Bsh1285I CGRYCG 2 cut(s) 331, 842
BshFI GGCC 2 cut(s) 161, 548
BshNI GGYRCC 1 cut(s) 1503
BsiEI CGRYCG 2 cut(s) 331, 842
BsiHKAI GWGCWC 2 cut(s) 102, 344
BsiSI CCGG 2 cut(s) 345, 1501
BslFI GGGAC 2 cut(s) 190, 1510
BslI CCNNNNNNNGG 2 cut(s) 1311, 1500
BsmFI GGGAC 2 cut(s) 190, 1510
BsmI GAATGC 2 cut(s) 812, 1150
BsnI GGCC 2 cut(s) 161, 548
Bsp1286I GDGCHC 3 cut(s) 52, 102, 344
Bsp1407I TGTACA 2 cut(s) 528, 686
Bsp143I GATC 5 cut(s) 105, 328, 430, 522, 625
Bsp19I CCATGG 1 cut(s) 775
BspACI CCGC 4 cut(s) 1103, 1105, 1247, 1379
BspANI GGCC 2 cut(s) 161, 548
BspCNI CTCAG 3 cut(s) 610, 755, 1618
BspFNI CGCG 1 cut(s) 1105
BspHI TCATGA 1 cut(s) 1140
BspLI GGNNCC 2 cut(s) 988, 1505
BspMI ACCTGC 1 cut(s) 925
BspOI GCTAGC 1 cut(s) 1607
BspPI GGATC 3 cut(s) 100, 438, 620
BspQI GCTCTTC 2 cut(s) 550, 1338
BspT107I GGYRCC 1 cut(s) 1503
BsrFI RCCGGY 1 cut(s) 1500
BsrGI TGTACA 2 cut(s) 528, 686
BsrI ACTGG 2 cut(s) 42, 999
BssAI RCCGGY 1 cut(s) 1500
BssECI CCNNGG 5 cut(s) 345, 444, 445, 775, 1103
BssMI GATC 5 cut(s) 105, 328, 430, 522, 625
BssT1I CCWWGG 1 cut(s) 775
Bst2UI CCWGG 1 cut(s) 446
Bst6I CTCTTC 5 cut(s) 95, 550, 1287, 1338, 1495
BstAUI TGTACA 2 cut(s) 528, 686
BstC8I GCNNGC 3 cut(s) 1158, 1377, 1605
BstDEI CTNAG 5 cut(s) 550, 597, 742, 1243, 1626
BstDSI CCRYGG 2 cut(s) 775, 1103
BstEII GGTNACC 1 cut(s) 1031
BstF5I GGATG 1 cut(s) 856
BstFNI CGCG 1 cut(s) 1105
BstKTI GATC 5 cut(s) 108, 331, 433, 525, 628
BstMBI GATC 5 cut(s) 105, 328, 430, 522, 625
BstMCI CGRYCG 2 cut(s) 331, 842
BstMWI GCNNNNNNNGC 5 cut(s) 339, 356, 931, 1016, 1345
BstNI CCWGG 1 cut(s) 446
BstNSI RCATGY 2 cut(s) 337, 1379
BstPI GGTNACC 1 cut(s) 1031
BstSCI CCNGG 2 cut(s) 344, 444
BstSFI CTRYAG 2 cut(s) 354, 1449
BstUI CGCG 1 cut(s) 1105
BstV1I GCAGC 1 cut(s) 1596
BstV2I GAAGAC 2 cut(s) 385, 458
BstXI CCANNNNNNTGG 1 cut(s) 427
Bsu36I CCTNAGG 1 cut(s) 550
BsuI GTATCC 1 cut(s) 1185
BsuRI GGCC 2 cut(s) 161, 548
BtgI CCRYGG 2 cut(s) 775, 1103
BtrI CACGTC 2 cut(s) 922, 1384
BtsCI GGATG 1 cut(s) 856
BtsI GCAGTG 2 cut(s) 1341, 1376
BtsIMutI CAGTG 6 cut(s) 186, 440, 538, 1133, 1341, 1376
BveI ACCTGC 1 cut(s) 925
Cac8I GCNNGC 3 cut(s) 1158, 1377, 1605
CaiI CAGNNNCTG 1 cut(s) 1427
CciI TCATGA 1 cut(s) 1140
Cfr10I RCCGGY 1 cut(s) 1500
Cfr42I CCGCGG 1 cut(s) 1106
Csp6I GTAC 7 cut(s) 529, 687, 829, 1124, 1329, 1611, 1631
CspCI CAANNNNNGTGG 2 cut(s) 244, 279
CviQI GTAC 7 cut(s) 529, 687, 829, 1124, 1329, 1611, 1631
DdeI CTNAG 5 cut(s) 550, 597, 742, 1243, 1626
DpnI GATC 5 cut(s) 107, 330, 432, 524, 627
DpnII GATC 5 cut(s) 105, 328, 430, 522, 625
Eam1104I CTCTTC 5 cut(s) 95, 550, 1287, 1338, 1495
EarI CTCTTC 5 cut(s) 95, 550, 1287, 1338, 1495
Eco130I CCWWGG 1 cut(s) 775
Eco24I GRGCYC 1 cut(s) 52
Eco57I CTGAAG 1 cut(s) 709
Eco81I CCTNAGG 1 cut(s) 550
Eco91I GGTNACC 1 cut(s) 1031
EcoO65I GGTNACC 1 cut(s) 1031
EcoRI GAATTC 1 cut(s) 679
EcoRII CCWGG 1 cut(s) 444
EcoT14I CCWWGG 1 cut(s) 775
EcoT22I ATGCAT 1 cut(s) 1150
EcoT38I GRGCYC 1 cut(s) 52
ErhI CCWWGG 1 cut(s) 775
FalI AAGNNNNNCTT 2 cut(s) 707, 739
FaqI GGGAC 2 cut(s) 190, 1510
FblI GTMKAC 2 cut(s) 670, 918
Fnu4HI GCNGC 1 cut(s) 1585
FokI GGATG 1 cut(s) 863
FriOI GRGCYC 1 cut(s) 52
Fsp4HI GCNGC 1 cut(s) 1585
FspBI CTAG 5 cut(s) 383, 405, 1298, 1604, 1608
GluI GCNGC 1 cut(s) 1585
GsuI CTGGAG 1 cut(s) 866
HaeIII GGCC 2 cut(s) 161, 548
HapII CCGG 2 cut(s) 345, 1501
HincII GTYRAC 1 cut(s) 801
HindII GTYRAC 1 cut(s) 801
HinfI GANTC 4 cut(s) 19, 440, 636, 1424
HpaII CCGG 2 cut(s) 345, 1501
HphI GGTGA 3 cut(s) 1013, 1043, 1663
Hpy166II GTNNAC 7 cut(s) 529, 645, 671, 801, 919, 1633, 1654
Hpy188I TCNGA 8 cut(s) 10, 540, 600, 728, 860, 1423, 1464, 1648
Hpy188III TCNNGA 5 cut(s) 1141, 1433, 1553, 1565, 1574
Hpy8I GTNNAC 7 cut(s) 529, 645, 671, 801, 919, 1633, 1654
Hpy99I CGWCG 1 cut(s) 902
HpyAV CCTTC 2 cut(s) 5, 1318
HpyCH4IV ACGT 4 cut(s) 921, 1178, 1383, 1667
HpyF10VI GCNNNNNNNGC 5 cut(s) 339, 356, 931, 1016, 1345
HpyF3I CTNAG 5 cut(s) 550, 597, 742, 1243, 1626
HpySE526I ACGT 4 cut(s) 921, 1178, 1383, 1667
KspI CCGCGG 1 cut(s) 1106
Kzo9I GATC 5 cut(s) 105, 328, 430, 522, 625
LguI GCTCTTC 2 cut(s) 550, 1338
LmnI GCTCC 2 cut(s) 29, 339
Lsp1109I GCAGC 1 cut(s) 1596
LweI GCATC 2 cut(s) 359, 1126
MaeI CTAG 5 cut(s) 383, 405, 1298, 1604, 1608
MaeII ACGT 4 cut(s) 921, 1178, 1383, 1667
MaeIII GTNAC 7 cut(s) 220, 1031, 1082, 1174, 1271, 1300, 1430
MalI GATC 5 cut(s) 107, 330, 432, 524, 627
MboI GATC 5 cut(s) 105, 328, 430, 522, 625
MhlI GDGCHC 3 cut(s) 52, 102, 344
MlyI GAGTC 1 cut(s) 28
MnlI CCTC 9 cut(s) 151, 466, 841, 1071, 1298, 1389, 1453, 1621, 1642
Mph1103I ATGCAT 1 cut(s) 1150
MroXI GAANNNNTTC 1 cut(s) 1560
MseI TTAA 1 cut(s) 714
MslI CAYNNNNRTG 2 cut(s) 229, 425
MspA1I CMGCKG 1 cut(s) 1105
MspI CCGG 2 cut(s) 345, 1501
MspR9I CCNGG 2 cut(s) 346, 446
Mva1269I GAATGC 2 cut(s) 812, 1150
MvaI CCWGG 1 cut(s) 446
MvnI CGCG 1 cut(s) 1105
MwoI GCNNNNNNNGC 5 cut(s) 339, 356, 931, 1016, 1345
NciI CCSGG 1 cut(s) 346
NcoI CCATGG 1 cut(s) 775
NdeII GATC 5 cut(s) 105, 328, 430, 522, 625
NheI GCTAGC 1 cut(s) 1603
NlaIV GGNNCC 2 cut(s) 988, 1505
NmuCI GTSAC 5 cut(s) 1031, 1082, 1174, 1300, 1430
NsiI ATGCAT 1 cut(s) 1150
NspI RCATGY 2 cut(s) 337, 1379
OliI CACNNNNGTG 1 cut(s) 425
PaeI GCATGC 1 cut(s) 1379
PagI TCATGA 1 cut(s) 1140
PaqCI CACCTGC 1 cut(s) 925
PasI CCCWGGG 1 cut(s) 445
PciSI GCTCTTC 2 cut(s) 550, 1338
PctI GAATGC 2 cut(s) 812, 1150
PdmI GAANNNNTTC 1 cut(s) 1560
PfeI GAWTC 3 cut(s) 440, 636, 1424
PkrI GCNGC 1 cut(s) 1586
Ple19I CGATCG 1 cut(s) 331
PleI GAGTC 1 cut(s) 27
PpsI GAGTC 1 cut(s) 27
PsiI TTATAA 2 cut(s) 1232, 1364
Psp6I CCWGG 1 cut(s) 444
PspEI GGTNACC 1 cut(s) 1031
PspGI CCWGG 1 cut(s) 444
PspN4I GGNNCC 2 cut(s) 988, 1505
PstNI CAGNNNCTG 1 cut(s) 1427
PvuI CGATCG 1 cut(s) 331
RsaI GTAC 7 cut(s) 530, 688, 830, 1125, 1330, 1612, 1632
RsaNI GTAC 7 cut(s) 529, 687, 829, 1124, 1329, 1611, 1631
RseI CAYNNNNRTG 2 cut(s) 229, 425
SacII CCGCGG 1 cut(s) 1106
SapI GCTCTTC 2 cut(s) 550, 1338
SaqAI TTAA 1 cut(s) 714
SatI GCNGC 1 cut(s) 1585
Sau3AI GATC 5 cut(s) 105, 328, 430, 522, 625
ScaI AGTACT 1 cut(s) 1612
SchI GAGTC 1 cut(s) 28
ScrFI CCNGG 2 cut(s) 346, 446
SduI GDGCHC 3 cut(s) 52, 102, 344
SfaNI GCATC 2 cut(s) 359, 1126
SfcI CTRYAG 2 cut(s) 354, 1449
Sfr303I CCGCGG 1 cut(s) 1106
SgrBI CCGCGG 1 cut(s) 1106
SmiMI CAYNNNNRTG 2 cut(s) 229, 425
SphI GCATGC 1 cut(s) 1379
SsiI CCGC 4 cut(s) 1103, 1105, 1247, 1379
SspI AATATT 1 cut(s) 244
SspMI CTAG 5 cut(s) 383, 405, 1298, 1604, 1608
StyD4I CCNGG 2 cut(s) 344, 444
StyI CCWWGG 1 cut(s) 775
TaiI ACGT 4 cut(s) 924, 1181, 1386, 1670
TaqI TCGA 3 cut(s) 156, 327, 1542
TatI WGTACW 4 cut(s) 528, 686, 828, 1610
TfiI GAWTC 3 cut(s) 440, 636, 1424
Tru1I TTAA 1 cut(s) 714
Tru9I TTAA 1 cut(s) 714
TscAI CASTG 6 cut(s) 186, 440, 538, 1140, 1341, 1376
TseFI GTSAC 5 cut(s) 1031, 1082, 1174, 1300, 1430
TseI GCWGC 1 cut(s) 1584
Tsp45I GTSAC 5 cut(s) 1031, 1082, 1174, 1300, 1430
TspGWI ACGGA 1 cut(s) 1547
TspRI CASTG 6 cut(s) 186, 440, 538, 1140, 1341, 1376
XapI RAATTY 3 cut(s) 679, 1318, 1641
XceI RCATGY 2 cut(s) 337, 1379
XcmI CCANNNNNNNNNTGG 2 cut(s) 49, 997
XmiI GTMKAC 2 cut(s) 670, 918
XmnI GAANNNNTTC 1 cut(s) 1560
XspI CTAG 5 cut(s) 383, 405, 1298, 1604, 1608
ZrmI AGTACT 1 cut(s) 1612
Zsp2I ATGCAT 1 cut(s) 1150
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.