RchiOBHm_Chr3g0469171

MLO-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
3
OG0002520
Physical Location & Seq
Reverse (-)
14979030 .. 14983513
4484 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ43502

Sequence Viewer

Length: 1626 bp
ATGGCAGTAGCCGTTGAAGGCACCACTTTGGAATACACACCGACATGGGCTCTTGCCACTGTTTGCTTCTTCTTCATCTCTGTCTCCATCTTGATTGAACACTTAATCCACCTCCTTGTGACATGGCTCAAAAACCATCGAAAAACCGCCTTAACTGATGCGGTGGAGAAGCTCAAAGCAGAGTTAATGCTTCTGGGATTTGTGTCACTTTTATTGGCGGTGACCCAAGATAGGATAGCGAAAATTTGTATACCTGCAAAGATAGGAGATATAATGCTTCCCTGTCGTAAAGCCGTGAATGAGACAGAAGTGAAAACCGTTGAGCACTATGTGAACAAATTTGCAAGAAACCTTAATTCTTCTGGGGTACATGTCACGTTTGACCAAATGCTCAGGCAAACGCAGAGGATACTCGCCGAGGAGGAGGCAGCAGCTGTTGAAGATTATTGCGGTGATGAAAAGGTTTCGTTGGTGACGAAAAACGCACTTCATCAGCTGCACTTGCTGATCTTTGCGCTTGCGGTTATGCATATTGTGTATAGTGTTCTAACCATGGCTTTGGGCAGGGCTAAGATGAGGCGCTGGGAAGCATGGGAGGAAGAAACACGAACGATGGAGTACCAAGTTGCCAATGACCCCAATCGATTCAGACTTACAAGACAAACAACATTTGGAAAAAGGCACATGAATTCGTGCACAGAGACTTCACTTCATCTATGGCTGAAATGCTTTTTCAGGCAATTCTATAATTCCGTAGCAAAAGTTGACTACTTCACCATGAGACACGGTTTTATATCGGCTCATCTATCAAGCAGGCACAACAACTTTGATTTTCAAAAATACATACAGCGATCCTTGGAGGAAGATTTCAAGACATTGGTTGGCATAAGCCCTGCAATGTGGTTTGTAGTAGCCATTTTTATGCTGGTGGATGTGTATGGTTGGCACGTATATCTTTGGCTATCCTATGTTCCACTCTTGGTCGTTCTGGTCCTAGGAACCAAACTTGAATACATTGTTGCAAAGATGGCGCTTCAAATTAAAGAACAGAACAATGTAATCATTGGAACACCTCTTGTTCGAGTAGACGATAATCTATTCTGGTTCGGGAAACCAAGATTTGTCTTGGTGCTTTTACATTACACTTTGTTCCTGAATGCATTTGAGCTTGCCTTCTTCGTTTGGGTTACAGCCCAATTTGGAGTAGGATCATGCTACCACGAGCACACCGTGATCATTGTTACAAGAGTTTTCTTAGCGGTGACCACCCAAGTCCTCTGCAGCTACATTACTCTTCCTCTGTATGCACTTGTAACACAGATGGGTTCACAATTCAAGGGTAAGATACTAGAAGATAACATGGCTGGCATTCTAAAGCAATGGCACAGTGATGTGAGGCGGAGAAGGAAGAGGGAAGAACAAGAATCACAATCTGCACGCACGTCTTTTTCCATGGAATGGAGCTCTGTGAGGCACAGCATTTCTTCTTATATGAGACCAAGCACTTCCAGACGAGGTGAAAATGCCGATTTTTCTAACAGATGGGATAACAAGGACCAGACAGTAGAATTAGAAGAAAGCTCCTCATCAAGGGGTCACAGAAGAAATAGTGATTATGGGACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0006952 GO:0008150 GO:0016020 GO:0044464 GO:0050896 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mlo

Protein Analysis

541

Amino Acids

62.61

Weight (kDa)

8.92

Isoelectric Point (pI)

44.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mlo PF03094 8 - 493 1.3e-193 Mlo family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 262
AccB1I GGYRCC 1 cut(s) 20
AccB7I CCANNNNNTGG 1 cut(s) 1458
AccI GTMKAC 2 cut(s) 250, 1086
AciI CCGC 7 cut(s) 147, 161, 218, 450, 521, 1259, 1399
AclWI GGATC 2 cut(s) 846, 1216
AcsI RAATTY 3 cut(s) 243, 338, 688
AdeI CACNNNGTG 2 cut(s) 331, 1231
AfaI GTAC 2 cut(s) 369, 620
AfiI CCNNNNNNNGG 3 cut(s) 231, 1458, 1590
AflIII ACRYGT 1 cut(s) 370
AgsI TTSAA 8 cut(s) 17, 98, 440, 836, 871, 1010, 1037, 1336
AjiI CACGTC 1 cut(s) 1443
AloI GAACNNNNNNTCC 2 cut(s) 90, 122
AluBI AGCT 7 cut(s) 172, 434, 496, 1168, 1284, 1464, 1581
AluI AGCT 7 cut(s) 172, 434, 496, 1168, 1284, 1464, 1581
Alw21I GWGCWC 4 cut(s) 327, 698, 1227, 1466
Alw26I GTCTC 5 cut(s) 88, 296, 695, 775, 1489
Alw44I GTGCAC 1 cut(s) 694
AlwI GGATC 2 cut(s) 846, 1216
AlwNI CAGNNNCTG 1 cut(s) 434
ApaLI GTGCAC 1 cut(s) 694
ApeKI GCWGC 4 cut(s) 428, 431, 496, 1281
ApoI RAATTY 3 cut(s) 243, 338, 688
AspA2I CCTAGG 1 cut(s) 994
AspLEI GCGC 3 cut(s) 517, 582, 1033
AspS9I GGNCC 2 cut(s) 991, 1555
AsuHPI GGTGA 6 cut(s) 232, 464, 484, 766, 1273, 1529
AvaII GGWCC 2 cut(s) 991, 1555
AvrII CCTAGG 1 cut(s) 994
BaeGI GKGCMC 1 cut(s) 698
BanI GGYRCC 1 cut(s) 20
BanII GRGCYC 2 cut(s) 52, 1466
BauI CACGAG 1 cut(s) 1220
Bbv12I GWGCWC 4 cut(s) 327, 698, 1227, 1466
BbvI GCAGC 4 cut(s) 440, 443, 483, 1293
BccI CCATC 6 cut(s) 95, 144, 607, 1021, 1315, 1536
BceAI ACGGC 1 cut(s) 278
BciVI GTATCC 1 cut(s) 402
BclI TGATCA 1 cut(s) 1233
BcoDI GTCTC 5 cut(s) 88, 296, 695, 775, 1489
BfaI CTAG 2 cut(s) 995, 1349
BfmI CTRYAG 1 cut(s) 1279
BfoI RGCGCY 2 cut(s) 583, 1034
BfuAI ACCTGC 1 cut(s) 262
BfuI GTATCC 1 cut(s) 402
BisI GCNGC 4 cut(s) 429, 432, 497, 1282
BlnI CCTAGG 1 cut(s) 994
BlsI GCNGC 4 cut(s) 430, 433, 498, 1283
Bme18I GGWCC 2 cut(s) 991, 1555
BmgBI CACGTC 1 cut(s) 1443
BmgT120I GGNCC 2 cut(s) 991, 1555
BmiI GGNNCC 2 cut(s) 22, 1000
BmsI GCATC 1 cut(s) 148
Bpu10I CCTNAGC 1 cut(s) 392
Bsa29I ATCGAT 1 cut(s) 643
BsaAI YACGTR 1 cut(s) 949
BsaI GGTCTC 1 cut(s) 1489
BsaJI CCNNGG 5 cut(s) 417, 552, 855, 994, 1452
Bsc4I CCNNNNNNNGG 3 cut(s) 231, 1458, 1590
Bse3DI GCAATG 2 cut(s) 903, 1385
BseCI ATCGAT 1 cut(s) 643
BseDI CCNNGG 5 cut(s) 417, 552, 855, 994, 1452
BseGI GGATG 1 cut(s) 937
BseLI CCNNNNNNNGG 3 cut(s) 231, 1458, 1590
BseMI GCAATG 2 cut(s) 903, 1385
BseMII CTCAG 1 cut(s) 406
BseRI GAGGAG 3 cut(s) 434, 437, 1573
BseSI GKGCMC 1 cut(s) 698
BseXI GCAGC 4 cut(s) 440, 443, 483, 1293
BseYI CCCAGC 1 cut(s) 582
BsgI GTGCAG 2 cut(s) 482, 1419
BshNI GGYRCC 1 cut(s) 20
BshVI ATCGAT 1 cut(s) 643
BsiHKAI GWGCWC 4 cut(s) 327, 698, 1227, 1466
BslI CCNNNNNNNGG 3 cut(s) 231, 1458, 1590
BsmAI GTCTC 5 cut(s) 88, 296, 695, 775, 1489
BsmI GAATGC 2 cut(s) 1162, 1368
Bso31I GGTCTC 1 cut(s) 1489
Bsp1286I GDGCHC 5 cut(s) 52, 327, 698, 1227, 1466
Bsp143I GATC 4 cut(s) 507, 851, 1208, 1233
Bsp19I CCATGG 2 cut(s) 552, 1452
BspACI CCGC 7 cut(s) 147, 161, 218, 450, 521, 1259, 1399
BspCNI CTCAG 1 cut(s) 405
BspDI ATCGAT 1 cut(s) 643
BspLI GGNNCC 2 cut(s) 22, 1000
BspMAI CTGCAG 1 cut(s) 1283
BspMI ACCTGC 1 cut(s) 262
BspPI GGATC 2 cut(s) 846, 1216
BspT107I GGYRCC 1 cut(s) 20
BspTNI GGTCTC 1 cut(s) 1489
BsrDI GCAATG 2 cut(s) 903, 1385
BssECI CCNNGG 5 cut(s) 417, 552, 855, 994, 1452
BssMI GATC 4 cut(s) 507, 851, 1208, 1233
BssNAI GTATAC 1 cut(s) 251
BssSI CACGAG 1 cut(s) 1220
BssT1I CCWWGG 4 cut(s) 552, 855, 994, 1452
Bst1107I GTATAC 1 cut(s) 251
Bst2BI CACGAG 1 cut(s) 1220
Bst4CI ACNGT 6 cut(s) 61, 319, 788, 1231, 1388, 1564
Bst6I CTCTTC 2 cut(s) 1299, 1403
BstBAI YACGTR 1 cut(s) 949
BstC8I GCNNGC 5 cut(s) 519, 815, 1170, 1366, 1438
BstDEI CTNAG 3 cut(s) 392, 570, 1255
BstDSI CCRYGG 2 cut(s) 552, 1452
BstEII GGTNACC 2 cut(s) 220, 1261
BstENI CCTNNNNNAGG 1 cut(s) 1588
BstF5I GGATG 1 cut(s) 937
BstH2I RGCGCY 2 cut(s) 583, 1034
BstHHI GCGC 3 cut(s) 517, 582, 1033
BstKTI GATC 4 cut(s) 510, 854, 1211, 1236
BstMAI GTCTC 5 cut(s) 88, 296, 695, 775, 1489
BstMBI GATC 4 cut(s) 507, 851, 1208, 1233
BstMWI GCNNNNNNNGC 2 cut(s) 502, 1028
BstNSI RCATGY 1 cut(s) 374
BstPI GGTNACC 2 cut(s) 220, 1261
BstSFI CTRYAG 1 cut(s) 1279
BstSLI GKGCMC 1 cut(s) 698
BstV1I GCAGC 4 cut(s) 440, 443, 483, 1293
BstXI CCANNNNNNTGG 1 cut(s) 559
BstZ17I GTATAC 1 cut(s) 251
Bsu15I ATCGAT 1 cut(s) 643
BsuI GTATCC 1 cut(s) 402
BsuTUI ATCGAT 1 cut(s) 643
BtgI CCRYGG 2 cut(s) 552, 1452
BtrI CACGTC 1 cut(s) 1443
BtsCI GGATG 1 cut(s) 937
BtsIMutI CAGTG 2 cut(s) 57, 1393
BveI ACCTGC 1 cut(s) 262
Cac8I GCNNGC 5 cut(s) 519, 815, 1170, 1366, 1438
CaiI CAGNNNCTG 1 cut(s) 434
CfoI GCGC 3 cut(s) 517, 582, 1033
Cfr13I GGNCC 2 cut(s) 991, 1555
ClaI ATCGAT 1 cut(s) 643
Csp6I GTAC 2 cut(s) 368, 619
CviQI GTAC 2 cut(s) 368, 619
DdeI CTNAG 3 cut(s) 392, 570, 1255
DpnI GATC 4 cut(s) 509, 853, 1210, 1235
DpnII GATC 4 cut(s) 507, 851, 1208, 1233
DraIII CACNNNGTG 2 cut(s) 331, 1231
Eam1104I CTCTTC 2 cut(s) 1299, 1403
EarI CTCTTC 2 cut(s) 1299, 1403
EciI GGCGGA 1 cut(s) 1414
Ecl136II GAGCTC 1 cut(s) 1464
Eco130I CCWWGG 4 cut(s) 552, 855, 994, 1452
Eco24I GRGCYC 2 cut(s) 52, 1466
Eco31I GGTCTC 1 cut(s) 1489
Eco47I GGWCC 2 cut(s) 991, 1555
Eco53kI GAGCTC 1 cut(s) 1464
Eco91I GGTNACC 2 cut(s) 220, 1261
EcoICRI GAGCTC 1 cut(s) 1464
EcoNI CCTNNNNNAGG 1 cut(s) 1588
EcoO65I GGTNACC 2 cut(s) 220, 1261
EcoRI GAATTC 1 cut(s) 688
EcoT14I CCWWGG 4 cut(s) 552, 855, 994, 1452
EcoT22I ATGCAT 2 cut(s) 531, 1162
EcoT38I GRGCYC 2 cut(s) 52, 1466
ErhI CCWWGG 4 cut(s) 552, 855, 994, 1452
FbaI TGATCA 1 cut(s) 1233
FblI GTMKAC 2 cut(s) 250, 1086
Fnu4HI GCNGC 4 cut(s) 429, 432, 497, 1282
FokI GGATG 1 cut(s) 944
FriOI GRGCYC 2 cut(s) 52, 1466
Fsp4HI GCNGC 4 cut(s) 429, 432, 497, 1282
FspBI CTAG 2 cut(s) 995, 1349
GlaI GCGC 3 cut(s) 516, 581, 1032
GluI GCNGC 4 cut(s) 429, 432, 497, 1282
GsaI CCCAGC 1 cut(s) 586
HaeII RGCGCY 2 cut(s) 583, 1034
HhaI GCGC 3 cut(s) 517, 582, 1033
Hin6I GCGC 3 cut(s) 515, 580, 1031
HinP1I GCGC 3 cut(s) 515, 580, 1031
HincII GTYRAC 1 cut(s) 766
HindII GTYRAC 1 cut(s) 766
HinfI GANTC 2 cut(s) 645, 1424
HphI GGTGA 6 cut(s) 232, 464, 484, 766, 1273, 1529
Hpy166II GTNNAC 6 cut(s) 251, 334, 696, 766, 1087, 1328
Hpy188I TCNGA 1 cut(s) 650
Hpy188III TCNNGA 5 cut(s) 91, 871, 1108, 1153, 1509
Hpy8I GTNNAC 6 cut(s) 251, 334, 696, 766, 1087, 1328
HpyAV CCTTC 3 cut(s) 11, 1183, 1398
HpyCH4III ACNGT 6 cut(s) 61, 319, 788, 1231, 1388, 1564
HpyCH4IV ACGT 3 cut(s) 377, 948, 1442
HpyF10VI GCNNNNNNNGC 2 cut(s) 502, 1028
HpyF3I CTNAG 3 cut(s) 392, 570, 1255
HpySE526I ACGT 3 cut(s) 377, 948, 1442
HspAI GCGC 3 cut(s) 515, 580, 1031
Ksp22I TGATCA 1 cut(s) 1233
Kzo9I GATC 4 cut(s) 507, 851, 1208, 1233
LmnI GCTCC 2 cut(s) 1461, 1586
Lsp1109I GCAGC 4 cut(s) 440, 443, 483, 1293
LweI GCATC 1 cut(s) 148
MaeI CTAG 2 cut(s) 995, 1349
MaeII ACGT 3 cut(s) 377, 948, 1442
MalI GATC 4 cut(s) 509, 853, 1210, 1235
MboI GATC 4 cut(s) 507, 851, 1208, 1233
MhlI GDGCHC 5 cut(s) 52, 327, 698, 1227, 1466
Mph1103I ATGCAT 2 cut(s) 531, 1162
MseI TTAA 5 cut(s) 104, 152, 185, 354, 1041
MslI CAYNNNNRTG 3 cut(s) 43, 920, 1389
MspA1I CMGCKG 2 cut(s) 434, 496
Mva1269I GAATGC 2 cut(s) 1162, 1368
MwoI GCNNNNNNNGC 2 cut(s) 502, 1028
NcoI CCATGG 2 cut(s) 552, 1452
NdeII GATC 4 cut(s) 507, 851, 1208, 1233
NlaIV GGNNCC 2 cut(s) 22, 1000
NmeAIII GCCGAG 1 cut(s) 442
NmuCI GTSAC 7 cut(s) 118, 204, 220, 373, 472, 1261, 1595
NsiI ATGCAT 2 cut(s) 531, 1162
NspI RCATGY 1 cut(s) 374
PciI ACATGT 1 cut(s) 370
PcsI WCGNNNNNNNCGW 2 cut(s) 473, 1227
PctI GAATGC 2 cut(s) 1162, 1368
PfeI GAWTC 2 cut(s) 645, 1424
PflMI CCANNNNNTGG 1 cut(s) 1458
PkrI GCNGC 4 cut(s) 430, 433, 498, 1283
Ppu21I YACGTR 1 cut(s) 949
PscI ACATGT 1 cut(s) 370
Psp124BI GAGCTC 1 cut(s) 1466
PspEI GGTNACC 2 cut(s) 220, 1261
PspFI CCCAGC 1 cut(s) 582
PspN4I GGNNCC 2 cut(s) 22, 1000
PspPI GGNCC 2 cut(s) 991, 1555
PstI CTGCAG 1 cut(s) 1283
PstNI CAGNNNCTG 1 cut(s) 434
PvuII CAGCTG 2 cut(s) 434, 496
RsaI GTAC 2 cut(s) 369, 620
RsaNI GTAC 2 cut(s) 368, 619
RseI CAYNNNNRTG 3 cut(s) 43, 920, 1389
SacI GAGCTC 1 cut(s) 1466
SaqAI TTAA 5 cut(s) 104, 152, 185, 354, 1041
SatI GCNGC 4 cut(s) 429, 432, 497, 1282
Sau3AI GATC 4 cut(s) 507, 851, 1208, 1233
Sau96I GGNCC 2 cut(s) 991, 1555
SduI GDGCHC 5 cut(s) 52, 327, 698, 1227, 1466
SfaNI GCATC 1 cut(s) 148
SfcI CTRYAG 1 cut(s) 1279
SinI GGWCC 2 cut(s) 991, 1555
SmiMI CAYNNNNRTG 3 cut(s) 43, 920, 1389
SsiI CCGC 7 cut(s) 147, 161, 218, 450, 521, 1259, 1399
SspMI CTAG 2 cut(s) 995, 1349
SstI GAGCTC 1 cut(s) 1466
StyI CCWWGG 4 cut(s) 552, 855, 994, 1452
TaaI ACNGT 6 cut(s) 61, 319, 788, 1231, 1388, 1564
TaiI ACGT 3 cut(s) 380, 951, 1445
TaqI TCGA 3 cut(s) 139, 643, 1081
TfiI GAWTC 2 cut(s) 645, 1424
Tru1I TTAA 5 cut(s) 104, 152, 185, 354, 1041
Tru9I TTAA 5 cut(s) 104, 152, 185, 354, 1041
TscAI CASTG 2 cut(s) 64, 1393
TseFI GTSAC 7 cut(s) 118, 204, 220, 373, 472, 1261, 1595
TseI GCWGC 4 cut(s) 428, 431, 496, 1281
Tsp45I GTSAC 7 cut(s) 118, 204, 220, 373, 472, 1261, 1595
TspDTI ATGAA 5 cut(s) 64, 471, 479, 701, 701
TspGWI ACGGA 1 cut(s) 742
TspRI CASTG 2 cut(s) 64, 1393
Van91I CCANNNNNTGG 1 cut(s) 1458
VneI GTGCAC 1 cut(s) 694
VpaK11BI GGWCC 2 cut(s) 991, 1555
XagI CCTNNNNNAGG 1 cut(s) 1588
XapI RAATTY 3 cut(s) 243, 338, 688
XceI RCATGY 1 cut(s) 374
XcmI CCANNNNNNNNNTGG 1 cut(s) 922
XmaJI CCTAGG 1 cut(s) 994
XmiI GTMKAC 2 cut(s) 250, 1086
XspI CTAG 2 cut(s) 995, 1349
Zsp2I ATGCAT 2 cut(s) 531, 1162
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.