FvH4_6g15590

MLO-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0002520
Physical Location & Seq
Reverse (-)
9689955 .. 9694797
4843 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g15590.t2

Sequence Viewer

Length: 1629 bp
ATGGCAGCAGCCAGCGCAGGCACCTCTCTGGAATACACACCGACATGGGCTCTTGCCACTGTTTGCTTCTTCTTCATCTCCGTCTCCATCTTGCTTGAACACTTGATATTCCTTCTTGTTTCATGGCTCAAAAGGCATAAGAAAACCGCTTTAACTGATGCTGTTGAGAAGCTCAAAGCAGAGTTGATGCTTCTGGGATTTGTTTCACTTTTATTGGCGGTGACCCAAGATAGGATAGCGAAAATATGCATACCTGAAAAGGTAGGAAACATAATGCTTCCATGTCGGAAATACGTGAATGAGACTACCGAAGTTGAGACTGTTGAGCACTATGTCAACAAATTTGCCGGAAACCTTAATTCTTCCAGTGCACATGTCATGTTTGAAGAAATGCTCCGGCGAACGCAGAGAATACTTGCCGAGGAGGAGGCAGAAGCTGTTGAGGATTATTGCGGTGATGGAAAAGTTTCGTTAGTGACGAAAAACGCGCTTCATCAGCTTCACTTGCTGATCTTTGCGCTTGCGGTTATGCATATTGTGTATAGCGTTCTAACCATGGCTTTGGGCAGGGCTAAGATGAGGCGCTGGGAAGCTTGGGAGGAAGAAACTCGAACAATGGAGTACCAAGTTGCCAATGATCCCAATCGATTCAGACTTACAAGACAAACAACATTTGGAAAACGGCACATGAATTCGTGTACAGAGACTTCATTTCATCTCTGGCTGAAATGCTTTTTCAGGCAATTCTATAATTCAGTAGCAAAAGTTGACTACTTCACCATGAGACACGGTTTTATATCGGCTCACCTATCAAACAGGCACAACAACTTTGATTTCCAAAAGTACATACAACGATCCTTGGAAGAAGATTTCAAGACATTGGTTGGGATCAGCCCTGCAATGTGGTTTGCAGTAGCCATTTTTATGCTGGTGGATGTCTACGGTTGGCACGTATATCTATGGCTATCATATGTTCCACTCTTGGTCGTTCTGGTCCTGGGAACCAAACTTGAATACATTGTTGCAAAGATGGCGCTTCAAATTAAAGAGCAGAACAATGTAATCATTGGAACACCTCTTGTTCGAGTAAACGATGATCTTTTCTGGTTTAAGAAACCAAGATTTGTCTTGATACTTCTACATTACACTTTGTTCCTGAATGCGTTTGAGCTTGCCTTCTTCGTTTGGGTTACCGCCCAATTTGGATTTAAATCGTGCTACCACGAGCACACTGTGATCATTGTTACAAGAATTTTCTTAGCAGTGACAACCCAAGTTCTCTGCAGCTACATTACTCTTCCACTCTATGCACTTGTGACACAGATGGGATCACAATTCAAGGGTAAGATACTAGAAGATAACATGGCTGGCATTCTAAAGCAATGGCACGGTGATGTGAGGAGGAAAAGGAGGAAGGAAGAACGAGAATCACAATCGGCACGCACGTCATTTTCCGTGGAATGGAGCTCCATGAGGAACAGCATTTCTTCTTACATGAGACCAAGCCCTTCCAGACAAGGTGAAAATGCCGATTTTTCTAACAGATGGGACAACAAGGAACAGATAGTAGAATTAGAAGAAAGTTCCTCATCAAGGGGTCAGATAAGAAGTAGTGATTTTAGGACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0006952 GO:0008150 GO:0016020 GO:0044464 GO:0050896 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mlo

Protein Analysis

543

Amino Acids

62.9

Weight (kDa)

8.98

Isoelectric Point (pI)

44.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mlo PF03094 8 - 405 1.7e-158 Mlo family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 20
AccI GTMKAC 1 cut(s) 939
AccII CGCG 1 cut(s) 488
AciI CCGC 5 cut(s) 147, 218, 453, 524, 1194
AclWI GGATC 4 cut(s) 632, 849, 896, 1336
AcsI RAATTY 3 cut(s) 341, 691, 1251
AdeI CACNNNGTG 1 cut(s) 1234
AfaI GTAC 3 cut(s) 623, 700, 845
AfiI CCNNNNNNNGG 3 cut(s) 231, 1461, 1593
AflIII ACRYGT 1 cut(s) 373
AgsI TTSAA 6 cut(s) 98, 386, 874, 1013, 1040, 1339
AjiI CACGTC 1 cut(s) 1446
AjnI CCWGG 1 cut(s) 996
AluBI AGCT 7 cut(s) 172, 437, 499, 593, 1171, 1287, 1467
AluI AGCT 7 cut(s) 172, 437, 499, 593, 1171, 1287, 1467
Alw21I GWGCWC 4 cut(s) 330, 373, 1230, 1469
Alw26I GTCTC 6 cut(s) 88, 296, 311, 698, 778, 1492
Alw44I GTGCAC 1 cut(s) 369
AlwI GGATC 4 cut(s) 632, 849, 896, 1336
AlwNI CAGNNNCTG 1 cut(s) 437
ApaLI GTGCAC 1 cut(s) 369
ApeKI GCWGC 3 cut(s) 5, 8, 1284
ApoI RAATTY 3 cut(s) 341, 691, 1251
Asp700I GAANNNNTTC 1 cut(s) 466
AspLEI GCGC 5 cut(s) 17, 490, 520, 585, 1036
AspS9I GGNCC 1 cut(s) 994
AsuHPI GGTGA 6 cut(s) 232, 467, 769, 797, 1403, 1532
AvaII GGWCC 1 cut(s) 994
BaeGI GKGCMC 1 cut(s) 373
BanI GGYRCC 1 cut(s) 20
BanII GRGCYC 2 cut(s) 52, 1469
BarI GAAGNNNNNNTAC 2 cut(s) 691, 723
BauI CACGAG 1 cut(s) 1223
Bbv12I GWGCWC 4 cut(s) 330, 373, 1230, 1469
BbvI GCAGC 3 cut(s) 17, 20, 1296
BccI CCATC 5 cut(s) 95, 452, 1024, 1318, 1539
BceAI ACGGC 1 cut(s) 698
BciT130I CCWGG 1 cut(s) 998
BclI TGATCA 1 cut(s) 1236
BcoDI GTCTC 6 cut(s) 88, 296, 311, 698, 778, 1492
BfaI CTAG 1 cut(s) 1352
BfmI CTRYAG 1 cut(s) 1282
BfoI RGCGCY 2 cut(s) 586, 1037
BisI GCNGC 3 cut(s) 6, 9, 1285
BlsI GCNGC 3 cut(s) 7, 10, 1286
Bme1390I CCNGG 1 cut(s) 998
Bme18I GGWCC 1 cut(s) 994
BmgBI CACGTC 1 cut(s) 1446
BmgT120I GGNCC 1 cut(s) 994
BmiI GGNNCC 2 cut(s) 22, 1003
BmrFI CCNGG 1 cut(s) 998
BmsI GCATC 2 cut(s) 148, 177
Bsa29I ATCGAT 1 cut(s) 646
BsaAI YACGTR 2 cut(s) 295, 952
BsaBI GATNNNNATC 2 cut(s) 642, 1210
BsaI GGTCTC 1 cut(s) 1492
BsaJI CCNNGG 5 cut(s) 420, 555, 858, 997, 1455
Bsc4I CCNNNNNNNGG 3 cut(s) 231, 1461, 1593
Bse1I ACTGG 1 cut(s) 366
Bse3DI GCAATG 2 cut(s) 906, 1388
Bse8I GATNNNNATC 2 cut(s) 642, 1210
BseBI CCWGG 1 cut(s) 998
BseCI ATCGAT 1 cut(s) 646
BseDI CCNNGG 5 cut(s) 420, 555, 858, 997, 1455
BseGI GGATG 1 cut(s) 940
BseJI GATNNNNATC 2 cut(s) 642, 1210
BseLI CCNNNNNNNGG 3 cut(s) 231, 1461, 1593
BseMI GCAATG 2 cut(s) 906, 1388
BseNI ACTGG 1 cut(s) 366
BseRI GAGGAG 3 cut(s) 437, 440, 1414
BseSI GKGCMC 1 cut(s) 373
BseXI GCAGC 3 cut(s) 17, 20, 1296
BseYI CCCAGC 1 cut(s) 585
Bsh1236I CGCG 1 cut(s) 488
BshNI GGYRCC 1 cut(s) 20
BshVI ATCGAT 1 cut(s) 646
BsiHKAI GWGCWC 4 cut(s) 330, 373, 1230, 1469
BsiSI CCGG 2 cut(s) 348, 397
BslFI GGGAC 1 cut(s) 1562
BslI CCNNNNNNNGG 3 cut(s) 231, 1461, 1593
BsmAI GTCTC 6 cut(s) 88, 296, 311, 698, 778, 1492
BsmBI CGTCTC 1 cut(s) 88
BsmFI GGGAC 1 cut(s) 1562
BsmI GAATGC 2 cut(s) 1165, 1371
Bso31I GGTCTC 1 cut(s) 1492
Bsp1286I GDGCHC 5 cut(s) 52, 330, 373, 1230, 1469
Bsp1407I TGTACA 1 cut(s) 698
Bsp143I GATC 7 cut(s) 510, 637, 854, 888, 1096, 1236, 1328
Bsp19I CCATGG 1 cut(s) 555
BspACI CCGC 5 cut(s) 147, 218, 453, 524, 1194
BspDI ATCGAT 1 cut(s) 646
BspFNI CGCG 1 cut(s) 488
BspLI GGNNCC 2 cut(s) 22, 1003
BspMAI CTGCAG 1 cut(s) 1286
BspPI GGATC 4 cut(s) 632, 849, 896, 1336
BspT107I GGYRCC 1 cut(s) 20
BspTNI GGTCTC 1 cut(s) 1492
BsrDI GCAATG 2 cut(s) 906, 1388
BsrGI TGTACA 1 cut(s) 698
BsrI ACTGG 1 cut(s) 366
BssECI CCNNGG 5 cut(s) 420, 555, 858, 997, 1455
BssMI GATC 7 cut(s) 510, 637, 854, 888, 1096, 1236, 1328
BssSI CACGAG 1 cut(s) 1223
BssT1I CCWWGG 2 cut(s) 555, 858
Bst2BI CACGAG 1 cut(s) 1223
Bst2UI CCWGG 1 cut(s) 998
Bst4CI ACNGT 6 cut(s) 61, 322, 791, 944, 1234, 1391
Bst6I CTCTTC 1 cut(s) 1302
BstAUI TGTACA 1 cut(s) 698
BstBAI YACGTR 2 cut(s) 295, 952
BstC8I GCNNGC 6 cut(s) 13, 19, 522, 1173, 1369, 1441
BstDEI CTNAG 2 cut(s) 573, 1258
BstDSI CCRYGG 2 cut(s) 555, 1455
BstEII GGTNACC 2 cut(s) 220, 1189
BstENI CCTNNNNNAGG 1 cut(s) 1591
BstF5I GGATG 1 cut(s) 940
BstFNI CGCG 1 cut(s) 488
BstH2I RGCGCY 2 cut(s) 586, 1037
BstHHI GCGC 5 cut(s) 17, 490, 520, 585, 1036
BstKTI GATC 7 cut(s) 513, 640, 857, 891, 1099, 1239, 1331
BstMAI GTCTC 6 cut(s) 88, 296, 311, 698, 778, 1492
BstMBI GATC 7 cut(s) 510, 637, 854, 888, 1096, 1236, 1328
BstMWI GCNNNNNNNGC 5 cut(s) 14, 133, 496, 505, 1031
BstNI CCWGG 1 cut(s) 998
BstNSI RCATGY 1 cut(s) 377
BstPI GGTNACC 2 cut(s) 220, 1189
BstSCI CCNGG 1 cut(s) 996
BstSFI CTRYAG 1 cut(s) 1282
BstSLI GKGCMC 1 cut(s) 373
BstUI CGCG 1 cut(s) 488
BstV1I GCAGC 3 cut(s) 17, 20, 1296
BstXI CCANNNNNNTGG 1 cut(s) 562
Bsu15I ATCGAT 1 cut(s) 646
BsuTUI ATCGAT 1 cut(s) 646
BtgI CCRYGG 2 cut(s) 555, 1455
BtrI CACGTC 1 cut(s) 1446
BtsCI GGATG 1 cut(s) 940
BtsI GCAGTG 1 cut(s) 1269
BtsIMutI CAGTG 4 cut(s) 57, 373, 1230, 1269
Cac8I GCNNGC 6 cut(s) 13, 19, 522, 1173, 1369, 1441
CaiI CAGNNNCTG 1 cut(s) 437
CfoI GCGC 5 cut(s) 17, 490, 520, 585, 1036
Cfr13I GGNCC 1 cut(s) 994
ClaI ATCGAT 1 cut(s) 646
Csp6I GTAC 3 cut(s) 622, 699, 844
CviQI GTAC 3 cut(s) 622, 699, 844
DdeI CTNAG 2 cut(s) 573, 1258
DpnI GATC 7 cut(s) 512, 639, 856, 890, 1098, 1238, 1330
DpnII GATC 7 cut(s) 510, 637, 854, 888, 1096, 1236, 1328
DraI TTTAAA 1 cut(s) 1210
DraIII CACNNNGTG 1 cut(s) 1234
Eam1104I CTCTTC 1 cut(s) 1302
EarI CTCTTC 1 cut(s) 1302
Ecl136II GAGCTC 1 cut(s) 1467
Eco130I CCWWGG 2 cut(s) 555, 858
Eco24I GRGCYC 2 cut(s) 52, 1469
Eco31I GGTCTC 1 cut(s) 1492
Eco47I GGWCC 1 cut(s) 994
Eco53kI GAGCTC 1 cut(s) 1467
Eco91I GGTNACC 2 cut(s) 220, 1189
EcoICRI GAGCTC 1 cut(s) 1467
EcoNI CCTNNNNNAGG 1 cut(s) 1591
EcoO65I GGTNACC 2 cut(s) 220, 1189
EcoRI GAATTC 1 cut(s) 691
EcoRII CCWGG 1 cut(s) 996
EcoT14I CCWWGG 2 cut(s) 555, 858
EcoT22I ATGCAT 2 cut(s) 251, 534
EcoT38I GRGCYC 2 cut(s) 52, 1469
ErhI CCWWGG 2 cut(s) 555, 858
Esp3I CGTCTC 1 cut(s) 88
FaqI GGGAC 1 cut(s) 1562
FauNDI CATATG 1 cut(s) 970
FbaI TGATCA 1 cut(s) 1236
FblI GTMKAC 1 cut(s) 939
Fnu4HI GCNGC 3 cut(s) 6, 9, 1285
FokI GGATG 1 cut(s) 947
FriOI GRGCYC 2 cut(s) 52, 1469
Fsp4HI GCNGC 3 cut(s) 6, 9, 1285
FspBI CTAG 1 cut(s) 1352
GlaI GCGC 5 cut(s) 16, 489, 519, 584, 1035
GluI GCNGC 3 cut(s) 6, 9, 1285
GsaI CCCAGC 1 cut(s) 589
HaeII RGCGCY 2 cut(s) 586, 1037
HapII CCGG 2 cut(s) 348, 397
HhaI GCGC 5 cut(s) 17, 490, 520, 585, 1036
Hin6I GCGC 5 cut(s) 15, 488, 518, 583, 1034
HinP1I GCGC 5 cut(s) 15, 488, 518, 583, 1034
HincII GTYRAC 2 cut(s) 337, 769
HindII GTYRAC 2 cut(s) 337, 769
HindIII AAGCTT 1 cut(s) 591
HinfI GANTC 2 cut(s) 648, 1427
HpaII CCGG 2 cut(s) 348, 397
HphI GGTGA 6 cut(s) 232, 467, 769, 797, 1403, 1532
Hpy166II GTNNAC 6 cut(s) 337, 371, 699, 769, 940, 1090
Hpy188I TCNGA 3 cut(s) 288, 653, 1602
Hpy188III TCNNGA 5 cut(s) 29, 874, 1129, 1156, 1512
Hpy8I GTNNAC 6 cut(s) 337, 371, 699, 769, 940, 1090
HpyAV CCTTC 4 cut(s) 122, 1186, 1408, 1518
HpyCH4III ACNGT 6 cut(s) 61, 322, 791, 944, 1234, 1391
HpyCH4IV ACGT 3 cut(s) 294, 951, 1445
HpyCH4V TGCA 8 cut(s) 249, 371, 532, 899, 911, 1025, 1284, 1310
HpyF10VI GCNNNNNNNGC 5 cut(s) 14, 133, 496, 505, 1031
HpyF3I CTNAG 2 cut(s) 573, 1258
HpySE526I ACGT 3 cut(s) 294, 951, 1445
HspAI GCGC 5 cut(s) 15, 488, 518, 583, 1034
Ksp22I TGATCA 1 cut(s) 1236
Kzo9I GATC 7 cut(s) 510, 637, 854, 888, 1096, 1236, 1328
LmnI GCTCC 3 cut(s) 399, 1464, 1472
Lsp1109I GCAGC 3 cut(s) 17, 20, 1296
LweI GCATC 2 cut(s) 148, 177
MaeI CTAG 1 cut(s) 1352
MaeII ACGT 3 cut(s) 294, 951, 1445
MaeIII GTNAC 6 cut(s) 220, 475, 1189, 1243, 1264, 1315
MalI GATC 7 cut(s) 512, 639, 856, 890, 1098, 1238, 1330
MboI GATC 7 cut(s) 510, 637, 854, 888, 1096, 1236, 1328
MhlI GDGCHC 5 cut(s) 52, 330, 373, 1230, 1469
MmeI TCCRAC 1 cut(s) 266
Mph1103I ATGCAT 2 cut(s) 251, 534
MroXI GAANNNNTTC 1 cut(s) 466
MseI TTAA 5 cut(s) 152, 357, 1044, 1110, 1209
MslI CAYNNNNRTG 3 cut(s) 43, 923, 1392
MspI CCGG 2 cut(s) 348, 397
MspR9I CCNGG 1 cut(s) 998
Mva1269I GAATGC 2 cut(s) 1165, 1371
MvaI CCWGG 1 cut(s) 998
MvnI CGCG 1 cut(s) 488
MwoI GCNNNNNNNGC 5 cut(s) 14, 133, 496, 505, 1031
NcoI CCATGG 1 cut(s) 555
NdeI CATATG 1 cut(s) 970
NdeII GATC 7 cut(s) 510, 637, 854, 888, 1096, 1236, 1328
NlaIV GGNNCC 2 cut(s) 22, 1003
NmeAIII GCCGAG 1 cut(s) 445
NmuCI GTSAC 4 cut(s) 220, 475, 1264, 1315
NsiI ATGCAT 2 cut(s) 251, 534
NspI RCATGY 1 cut(s) 377
PciI ACATGT 1 cut(s) 373
PcsI WCGNNNNNNNCGW 3 cut(s) 476, 948, 1442
PctI GAATGC 2 cut(s) 1165, 1371
PdmI GAANNNNTTC 1 cut(s) 466
PfeI GAWTC 2 cut(s) 648, 1427
PkrI GCNGC 3 cut(s) 7, 10, 1286
Ppu21I YACGTR 2 cut(s) 295, 952
PscI ACATGT 1 cut(s) 373
Psp124BI GAGCTC 1 cut(s) 1469
Psp6I CCWGG 1 cut(s) 996
PspEI GGTNACC 2 cut(s) 220, 1189
PspFI CCCAGC 1 cut(s) 585
PspGI CCWGG 1 cut(s) 996
PspN4I GGNNCC 2 cut(s) 22, 1003
PspPI GGNCC 1 cut(s) 994
PstI CTGCAG 1 cut(s) 1286
PstNI CAGNNNCTG 1 cut(s) 437
RsaI GTAC 3 cut(s) 623, 700, 845
RsaNI GTAC 3 cut(s) 622, 699, 844
RseI CAYNNNNRTG 3 cut(s) 43, 923, 1392
SacI GAGCTC 1 cut(s) 1469
SaqAI TTAA 5 cut(s) 152, 357, 1044, 1110, 1209
SatI GCNGC 3 cut(s) 6, 9, 1285
Sau3AI GATC 7 cut(s) 510, 637, 854, 888, 1096, 1236, 1328
Sau96I GGNCC 1 cut(s) 994
ScrFI CCNGG 1 cut(s) 998
SduI GDGCHC 5 cut(s) 52, 330, 373, 1230, 1469
SfaNI GCATC 2 cut(s) 148, 177
SfcI CTRYAG 1 cut(s) 1282
SinI GGWCC 1 cut(s) 994
SmiI ATTTAAAT 1 cut(s) 1210
SmiMI CAYNNNNRTG 3 cut(s) 43, 923, 1392
SsiI CCGC 5 cut(s) 147, 218, 453, 524, 1194
SspMI CTAG 1 cut(s) 1352
SstI GAGCTC 1 cut(s) 1469
StyD4I CCNGG 1 cut(s) 996
StyI CCWWGG 2 cut(s) 555, 858
SwaI ATTTAAAT 1 cut(s) 1210
TaaI ACNGT 6 cut(s) 61, 322, 791, 944, 1234, 1391
TaiI ACGT 3 cut(s) 297, 954, 1448
TaqI TCGA 3 cut(s) 610, 646, 1084
TatI WGTACW 2 cut(s) 698, 843
TfiI GAWTC 2 cut(s) 648, 1427
Tru1I TTAA 5 cut(s) 152, 357, 1044, 1110, 1209
Tru9I TTAA 5 cut(s) 152, 357, 1044, 1110, 1209
TscAI CASTG 4 cut(s) 64, 373, 1237, 1269
TseFI GTSAC 4 cut(s) 220, 475, 1264, 1315
TseI GCWGC 3 cut(s) 5, 8, 1284
Tsp45I GTSAC 4 cut(s) 220, 475, 1264, 1315
TspDTI ATGAA 6 cut(s) 64, 111, 482, 699, 704, 704
TspGWI ACGGA 2 cut(s) 70, 1444
TspRI CASTG 4 cut(s) 64, 373, 1237, 1269
VneI GTGCAC 1 cut(s) 369
VpaK11BI GGWCC 1 cut(s) 994
XagI CCTNNNNNAGG 1 cut(s) 1591
XapI RAATTY 3 cut(s) 341, 691, 1251
XceI RCATGY 1 cut(s) 377
XcmI CCANNNNNNNNNTGG 1 cut(s) 925
XmiI GTMKAC 1 cut(s) 939
XmnI GAANNNNTTC 1 cut(s) 466
XspI CTAG 1 cut(s) 1352
Zsp2I ATGCAT 2 cut(s) 251, 534
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.