Rroxscaffold_6G00412140

MLO-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0002520
Physical Location & Seq
Forward (+)
34761739 .. 34766207
4469 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00412140.1

Sequence Viewer

Length: 1629 bp
ATGGCAGTAGCCGTTGAAGGCACCACTTTGGAATACACACCGACATGGGCTCTTGCCACTGTTTGCTTCTTCTTCATCTCCGTCTCCATCTTGATTGAACACTTAATCCACCTCCTTGTAACATGGCTCAAAAACCATCGAAAAACCGCCTTAACTGATGCGGTTGAGAAGCTCAAAGCAGAGTTGATGCTTCTGGGATTTGTGTCACTTTTATTGGCGGTGACCCAAGATAGGATAGCGAAAATTTGTATACCTGCAAAGATAGGAGATATAATGCTTCCATGTCGTAAAGCCTCGAATAAGACAGCCGAAGTGGAAACTGTTGAGCACTATGTGAACAAATTTGCAGGAAACCTTAATTCTTCTGGGGCACATGTCATGTTTGACCAAATGTTCAGGCAAACGCAGAGGATACTCGCCGAGGAGGAGGCAGCAGCTGTTGAGGATTATTGCGGTGATGGAAAGGTTTCATTGGTGACGAAAAACGCCCTTCATCAGCTGCACTTGCTGATCTTTGCGCTTGCTGTTATGCATATTGTGTATAGTGTTCTAACCATGGCTTTGGGCAGGGCTAAGATGAGGCGCTGGGAAGCATGGGAGGAAGAAACTCGAACGATGGAGTACCAAGTTGCCAATGATCCCAATCGATTCAGACTTACGAGACAAACAACATTTGGACAAAGGCACATGAATTCGTGCACAGAGACTTCACTTCATCTATGGCTGAAATGCTTTTTCAGGCAATTCTATAATTCCGTAGCAAAAGTTGACTACTTCACCATGAGACACGGTTTTATATCGGCTCACCTATCAAGCAGACACAACAACTTTGATTTTCAAAAATACATACAGCGATCCTTGGAGGAAGATTTCAAGACATTGGTTGGCATAAGCCCTGCAATGTGGTTTGTAGTAGCCATTTTTATGCTGGTGGATGTGTATGGTTGGCATGTATATCTTTGGCTATCCTATGTTCCACTCTTGGTCGTTCTGGTCCTAGGAACCAAACTTGAATACATTGTTGCAAAGATGGCGCTTCAAATTAAAGAACAGAACAATGTAATCATTGGAACACCTCTTGTTCGAGTAGACGATAATCTATTCTGGTTCGGGAAACCAAGATTTGTCTTGGTGCTTTTACATTATACTTTGTTCCTGAATGCATTTGAGCTTGCCTTCTTCGTTTGGGTTACAGCCCAATTTGGAGTAGGATCATGCTACCACGAGCACACCGTGATCATTGTTACAAGAGTTTTCTTAGCGGTGACCACCCAAGTCCTCTGCAGCTACATTACTCTTCCTCTGTATGCACTTGTAACACAGATGGGTTCACAATTCAAGGGTAAGATACTAGAAGATAACATGGCTGGCATTCTAAAGCAATGGCACGGTGATGTGAGGCGGAGAAGGAAGAAGGAAGAACAAGAATCACAATCTGCACGCACGTCTTTTTCCATGGAATGGAGCTCTGTGAGGCACAGCATTTCTTCTTATATGAGACCAAGCACTTCCAGACGAGGTGAAAATGCCGATTTTTCTAACAGATGGGATAACAAGGACCAGATAGTAGAATTAGAAGAAAGCTCCTCATCAAGGGGTCACAGAAGAAATAGTGATTATGGGACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0006952 GO:0008150 GO:0016020 GO:0044464 GO:0050896 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mlo

Protein Analysis

542

Amino Acids

62.49

Weight (kDa)

8.83

Isoelectric Point (pI)

41.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mlo PF03094 8 - 494 7.1e-195 Mlo family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 262
AccB1I GGYRCC 1 cut(s) 20
AccB7I CCANNNNNTGG 1 cut(s) 1461
AccI GTMKAC 2 cut(s) 250, 1089
AciI CCGC 6 cut(s) 147, 161, 218, 453, 1262, 1402
AclWI GGATC 3 cut(s) 632, 849, 1219
AcsI RAATTY 3 cut(s) 243, 341, 691
AdeI CACNNNGTG 2 cut(s) 334, 1234
AfaI GTAC 1 cut(s) 623
AfiI CCNNNNNNNGG 3 cut(s) 231, 1461, 1593
AflIII ACRYGT 1 cut(s) 373
AgsI TTSAA 7 cut(s) 17, 98, 839, 874, 1013, 1040, 1339
AjiI CACGTC 1 cut(s) 1446
AloI GAACNNNNNNTCC 2 cut(s) 90, 122
AluBI AGCT 7 cut(s) 172, 437, 499, 1171, 1287, 1467, 1584
AluI AGCT 7 cut(s) 172, 437, 499, 1171, 1287, 1467, 1584
Alw21I GWGCWC 4 cut(s) 330, 701, 1230, 1469
Alw26I GTCTC 5 cut(s) 88, 655, 698, 778, 1492
Alw44I GTGCAC 1 cut(s) 697
AlwI GGATC 3 cut(s) 632, 849, 1219
AlwNI CAGNNNCTG 1 cut(s) 437
ApaLI GTGCAC 1 cut(s) 697
ApeKI GCWGC 4 cut(s) 431, 434, 499, 1284
ApoI RAATTY 3 cut(s) 243, 341, 691
Asp700I GAANNNNTTC 1 cut(s) 466
AspA2I CCTAGG 1 cut(s) 997
AspLEI GCGC 3 cut(s) 520, 585, 1036
AspS9I GGNCC 2 cut(s) 994, 1558
AsuHPI GGTGA 8 cut(s) 232, 467, 487, 769, 797, 1276, 1403, 1532
AvaII GGWCC 2 cut(s) 994, 1558
AvrII CCTAGG 1 cut(s) 997
BaeGI GKGCMC 2 cut(s) 373, 701
BanI GGYRCC 1 cut(s) 20
BanII GRGCYC 2 cut(s) 52, 1469
BauI CACGAG 1 cut(s) 1223
Bbv12I GWGCWC 4 cut(s) 330, 701, 1230, 1469
BbvI GCAGC 4 cut(s) 443, 446, 486, 1296
BccI CCATC 7 cut(s) 95, 144, 452, 610, 1024, 1318, 1539
BciVI GTATCC 1 cut(s) 405
BclI TGATCA 1 cut(s) 1236
BcoDI GTCTC 5 cut(s) 88, 655, 698, 778, 1492
BfaI CTAG 2 cut(s) 998, 1352
BfmI CTRYAG 1 cut(s) 1282
BfoI RGCGCY 2 cut(s) 586, 1037
BfuAI ACCTGC 1 cut(s) 262
BfuI GTATCC 1 cut(s) 405
BisI GCNGC 4 cut(s) 432, 435, 500, 1285
BlnI CCTAGG 1 cut(s) 997
BlsI GCNGC 4 cut(s) 433, 436, 501, 1286
Bme18I GGWCC 2 cut(s) 994, 1558
BmgBI CACGTC 1 cut(s) 1446
BmgT120I GGNCC 2 cut(s) 994, 1558
BmiI GGNNCC 2 cut(s) 22, 1003
BmsI GCATC 2 cut(s) 148, 177
Bsa29I ATCGAT 1 cut(s) 646
BsaBI GATNNNNATC 1 cut(s) 642
BsaI GGTCTC 1 cut(s) 1492
BsaJI CCNNGG 5 cut(s) 420, 555, 858, 997, 1455
Bsc4I CCNNNNNNNGG 3 cut(s) 231, 1461, 1593
Bse3DI GCAATG 2 cut(s) 906, 1388
Bse8I GATNNNNATC 1 cut(s) 642
BseCI ATCGAT 1 cut(s) 646
BseDI CCNNGG 5 cut(s) 420, 555, 858, 997, 1455
BseGI GGATG 1 cut(s) 940
BseJI GATNNNNATC 1 cut(s) 642
BseLI CCNNNNNNNGG 3 cut(s) 231, 1461, 1593
BseMI GCAATG 2 cut(s) 906, 1388
BseRI GAGGAG 3 cut(s) 437, 440, 1576
BseSI GKGCMC 2 cut(s) 373, 701
BseXI GCAGC 4 cut(s) 443, 446, 486, 1296
BseYI CCCAGC 1 cut(s) 585
BsgI GTGCAG 2 cut(s) 485, 1422
BshNI GGYRCC 1 cut(s) 20
BshVI ATCGAT 1 cut(s) 646
BsiHKAI GWGCWC 4 cut(s) 330, 701, 1230, 1469
BslI CCNNNNNNNGG 3 cut(s) 231, 1461, 1593
BsmAI GTCTC 5 cut(s) 88, 655, 698, 778, 1492
BsmBI CGTCTC 1 cut(s) 88
BsmI GAATGC 2 cut(s) 1165, 1371
Bso31I GGTCTC 1 cut(s) 1492
Bsp1286I GDGCHC 6 cut(s) 52, 330, 373, 701, 1230, 1469
Bsp143I GATC 5 cut(s) 510, 637, 854, 1211, 1236
Bsp19I CCATGG 2 cut(s) 555, 1455
BspACI CCGC 6 cut(s) 147, 161, 218, 453, 1262, 1402
BspDI ATCGAT 1 cut(s) 646
BspLI GGNNCC 2 cut(s) 22, 1003
BspMAI CTGCAG 1 cut(s) 1286
BspMI ACCTGC 1 cut(s) 262
BspPI GGATC 3 cut(s) 632, 849, 1219
BspT107I GGYRCC 1 cut(s) 20
BspTNI GGTCTC 1 cut(s) 1492
BsrDI GCAATG 2 cut(s) 906, 1388
BssECI CCNNGG 5 cut(s) 420, 555, 858, 997, 1455
BssMI GATC 5 cut(s) 510, 637, 854, 1211, 1236
BssNAI GTATAC 1 cut(s) 251
BssSI CACGAG 1 cut(s) 1223
BssT1I CCWWGG 4 cut(s) 555, 858, 997, 1455
Bst1107I GTATAC 1 cut(s) 251
Bst2BI CACGAG 1 cut(s) 1223
Bst4CI ACNGT 5 cut(s) 61, 322, 791, 1234, 1391
Bst6I CTCTTC 1 cut(s) 1302
BstC8I GCNNGC 4 cut(s) 522, 1173, 1369, 1441
BstDEI CTNAG 2 cut(s) 573, 1258
BstDSI CCRYGG 2 cut(s) 555, 1455
BstEII GGTNACC 2 cut(s) 220, 1264
BstENI CCTNNNNNAGG 1 cut(s) 1591
BstF5I GGATG 1 cut(s) 940
BstH2I RGCGCY 2 cut(s) 586, 1037
BstHHI GCGC 3 cut(s) 520, 585, 1036
BstKTI GATC 5 cut(s) 513, 640, 857, 1214, 1239
BstMAI GTCTC 5 cut(s) 88, 655, 698, 778, 1492
BstMBI GATC 5 cut(s) 510, 637, 854, 1211, 1236
BstMWI GCNNNNNNNGC 2 cut(s) 505, 1031
BstNSI RCATGY 2 cut(s) 377, 953
BstPI GGTNACC 2 cut(s) 220, 1264
BstSFI CTRYAG 1 cut(s) 1282
BstSLI GKGCMC 2 cut(s) 373, 701
BstV1I GCAGC 4 cut(s) 443, 446, 486, 1296
BstXI CCANNNNNNTGG 1 cut(s) 562
BstZ17I GTATAC 1 cut(s) 251
Bsu15I ATCGAT 1 cut(s) 646
BsuI GTATCC 1 cut(s) 405
BsuTUI ATCGAT 1 cut(s) 646
BtgI CCRYGG 2 cut(s) 555, 1455
BtrI CACGTC 1 cut(s) 1446
BtsCI GGATG 1 cut(s) 940
BtsIMutI CAGTG 1 cut(s) 57
BveI ACCTGC 1 cut(s) 262
Cac8I GCNNGC 4 cut(s) 522, 1173, 1369, 1441
CaiI CAGNNNCTG 1 cut(s) 437
CfoI GCGC 3 cut(s) 520, 585, 1036
Cfr13I GGNCC 2 cut(s) 994, 1558
ClaI ATCGAT 1 cut(s) 646
Csp6I GTAC 1 cut(s) 622
CviQI GTAC 1 cut(s) 622
DdeI CTNAG 2 cut(s) 573, 1258
DpnI GATC 5 cut(s) 512, 639, 856, 1213, 1238
DpnII GATC 5 cut(s) 510, 637, 854, 1211, 1236
DraIII CACNNNGTG 2 cut(s) 334, 1234
Eam1104I CTCTTC 1 cut(s) 1302
EarI CTCTTC 1 cut(s) 1302
EciI GGCGGA 1 cut(s) 1417
Ecl136II GAGCTC 1 cut(s) 1467
Eco130I CCWWGG 4 cut(s) 555, 858, 997, 1455
Eco24I GRGCYC 2 cut(s) 52, 1469
Eco31I GGTCTC 1 cut(s) 1492
Eco47I GGWCC 2 cut(s) 994, 1558
Eco53kI GAGCTC 1 cut(s) 1467
Eco91I GGTNACC 2 cut(s) 220, 1264
EcoICRI GAGCTC 1 cut(s) 1467
EcoNI CCTNNNNNAGG 1 cut(s) 1591
EcoO65I GGTNACC 2 cut(s) 220, 1264
EcoRI GAATTC 1 cut(s) 691
EcoT14I CCWWGG 4 cut(s) 555, 858, 997, 1455
EcoT22I ATGCAT 2 cut(s) 534, 1165
EcoT38I GRGCYC 2 cut(s) 52, 1469
ErhI CCWWGG 4 cut(s) 555, 858, 997, 1455
Esp3I CGTCTC 1 cut(s) 88
FbaI TGATCA 1 cut(s) 1236
FblI GTMKAC 2 cut(s) 250, 1089
Fnu4HI GCNGC 4 cut(s) 432, 435, 500, 1285
FokI GGATG 1 cut(s) 947
FriOI GRGCYC 2 cut(s) 52, 1469
Fsp4HI GCNGC 4 cut(s) 432, 435, 500, 1285
FspBI CTAG 2 cut(s) 998, 1352
GlaI GCGC 3 cut(s) 519, 584, 1035
GluI GCNGC 4 cut(s) 432, 435, 500, 1285
GsaI CCCAGC 1 cut(s) 589
HaeII RGCGCY 2 cut(s) 586, 1037
HhaI GCGC 3 cut(s) 520, 585, 1036
Hin6I GCGC 3 cut(s) 518, 583, 1034
HinP1I GCGC 3 cut(s) 518, 583, 1034
HincII GTYRAC 1 cut(s) 769
HindII GTYRAC 1 cut(s) 769
HinfI GANTC 2 cut(s) 648, 1427
HphI GGTGA 8 cut(s) 232, 467, 487, 769, 797, 1276, 1403, 1532
Hpy166II GTNNAC 6 cut(s) 251, 337, 699, 769, 1090, 1331
Hpy188I TCNGA 1 cut(s) 653
Hpy188III TCNNGA 5 cut(s) 91, 874, 1111, 1156, 1512
Hpy8I GTNNAC 6 cut(s) 251, 337, 699, 769, 1090, 1331
HpyAV CCTTC 5 cut(s) 11, 500, 1186, 1401, 1408
HpyCH4III ACNGT 5 cut(s) 61, 322, 791, 1234, 1391
HpyCH4IV ACGT 1 cut(s) 1445
HpyF10VI GCNNNNNNNGC 2 cut(s) 505, 1031
HpyF3I CTNAG 2 cut(s) 573, 1258
HpySE526I ACGT 1 cut(s) 1445
HspAI GCGC 3 cut(s) 518, 583, 1034
Ksp22I TGATCA 1 cut(s) 1236
Kzo9I GATC 5 cut(s) 510, 637, 854, 1211, 1236
LmnI GCTCC 2 cut(s) 1464, 1589
Lsp1109I GCAGC 4 cut(s) 443, 446, 486, 1296
LweI GCATC 2 cut(s) 148, 177
MaeI CTAG 2 cut(s) 998, 1352
MaeII ACGT 1 cut(s) 1445
MaeIII GTNAC 9 cut(s) 118, 204, 220, 475, 1189, 1243, 1264, 1315, 1598
MalI GATC 5 cut(s) 512, 639, 856, 1213, 1238
MboI GATC 5 cut(s) 510, 637, 854, 1211, 1236
MhlI GDGCHC 6 cut(s) 52, 330, 373, 701, 1230, 1469
Mph1103I ATGCAT 2 cut(s) 534, 1165
MroXI GAANNNNTTC 1 cut(s) 466
MseI TTAA 4 cut(s) 104, 152, 357, 1044
MslI CAYNNNNRTG 3 cut(s) 43, 923, 1392
MspA1I CMGCKG 2 cut(s) 437, 499
Mva1269I GAATGC 2 cut(s) 1165, 1371
MwoI GCNNNNNNNGC 2 cut(s) 505, 1031
NcoI CCATGG 2 cut(s) 555, 1455
NdeII GATC 5 cut(s) 510, 637, 854, 1211, 1236
NlaIV GGNNCC 2 cut(s) 22, 1003
NmeAIII GCCGAG 1 cut(s) 445
NmuCI GTSAC 5 cut(s) 204, 220, 475, 1264, 1598
NsiI ATGCAT 2 cut(s) 534, 1165
NspI RCATGY 2 cut(s) 377, 953
PciI ACATGT 1 cut(s) 373
PcsI WCGNNNNNNNCGW 1 cut(s) 1230
PctI GAATGC 2 cut(s) 1165, 1371
PdmI GAANNNNTTC 1 cut(s) 466
PfeI GAWTC 2 cut(s) 648, 1427
PflMI CCANNNNNTGG 1 cut(s) 1461
PkrI GCNGC 4 cut(s) 433, 436, 501, 1286
PscI ACATGT 1 cut(s) 373
Psp124BI GAGCTC 1 cut(s) 1469
PspEI GGTNACC 2 cut(s) 220, 1264
PspFI CCCAGC 1 cut(s) 585
PspN4I GGNNCC 2 cut(s) 22, 1003
PspPI GGNCC 2 cut(s) 994, 1558
PstI CTGCAG 1 cut(s) 1286
PstNI CAGNNNCTG 1 cut(s) 437
PvuII CAGCTG 2 cut(s) 437, 499
RsaI GTAC 1 cut(s) 623
RsaNI GTAC 1 cut(s) 622
RseI CAYNNNNRTG 3 cut(s) 43, 923, 1392
SacI GAGCTC 1 cut(s) 1469
SaqAI TTAA 4 cut(s) 104, 152, 357, 1044
SatI GCNGC 4 cut(s) 432, 435, 500, 1285
Sau3AI GATC 5 cut(s) 510, 637, 854, 1211, 1236
Sau96I GGNCC 2 cut(s) 994, 1558
SduI GDGCHC 6 cut(s) 52, 330, 373, 701, 1230, 1469
SfaNI GCATC 2 cut(s) 148, 177
SfcI CTRYAG 1 cut(s) 1282
SinI GGWCC 2 cut(s) 994, 1558
SmiMI CAYNNNNRTG 3 cut(s) 43, 923, 1392
SsiI CCGC 6 cut(s) 147, 161, 218, 453, 1262, 1402
SspMI CTAG 2 cut(s) 998, 1352
SstI GAGCTC 1 cut(s) 1469
StyI CCWWGG 4 cut(s) 555, 858, 997, 1455
TaaI ACNGT 5 cut(s) 61, 322, 791, 1234, 1391
TaiI ACGT 1 cut(s) 1448
TaqI TCGA 5 cut(s) 139, 296, 610, 646, 1084
TfiI GAWTC 2 cut(s) 648, 1427
Tru1I TTAA 4 cut(s) 104, 152, 357, 1044
Tru9I TTAA 4 cut(s) 104, 152, 357, 1044
TscAI CASTG 1 cut(s) 64
TseFI GTSAC 5 cut(s) 204, 220, 475, 1264, 1598
TseI GCWGC 4 cut(s) 431, 434, 499, 1284
Tsp45I GTSAC 5 cut(s) 204, 220, 475, 1264, 1598
TspDTI ATGAA 5 cut(s) 64, 459, 482, 704, 704
TspGWI ACGGA 2 cut(s) 70, 745
TspRI CASTG 1 cut(s) 64
Van91I CCANNNNNTGG 1 cut(s) 1461
VneI GTGCAC 1 cut(s) 697
VpaK11BI GGWCC 2 cut(s) 994, 1558
XagI CCTNNNNNAGG 1 cut(s) 1591
XapI RAATTY 3 cut(s) 243, 341, 691
XceI RCATGY 2 cut(s) 377, 953
XcmI CCANNNNNNNNNTGG 1 cut(s) 925
XmaJI CCTAGG 1 cut(s) 997
XmiI GTMKAC 2 cut(s) 250, 1089
XmnI GAANNNNTTC 1 cut(s) 466
XspI CTAG 2 cut(s) 998, 1352
Zsp2I ATGCAT 2 cut(s) 534, 1165
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.