MD04G1101400.v1.1

MLO-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0002520
Physical Location & Seq
Forward (+)
18781275 .. 18784008
2734 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1101400.v1.1.491

Sequence Viewer

Length: 702 bp
ATGGCAGGTGATTCCAAAGGCGCCTCTCTGGAATACACTCCGACATGGGCACTCGCCACCGTTTGCTTCATCTTCATCTTCACCTCCCTCGCCATTGAGCACTCCATCCATCTCCTTGTCAACTTGATGCTTATGGGATTTGTGTCACTTTTATTAGCAATAACCCAAGATTCGATATCTAAAATCTGCATACCTGCCAACCTGGGAAACATCATGCTTCCATGTCGCAGAAAGGAAACAACCGAAGAAGCAGACGACGTCGAGAAATTTGTGATTTTGAATAAGATTATGACAACCTTTTCGACCGGCTCAAACAGTTTGTATGATGAAGTACTAAGGCAAATATCAGTGCATAGGCGACTTGAAGAGAAGGAGGATGCAGCTGCTGCAGATTCTTGCAGTGAGGGAAAAGTACATTTCATGACTACAAATGCTCTTCGTCAGCTTCACATACTTATTTTTGTGCTGCCAGTTATGCATATTGTGTACAGTGTTCTTACCATGGCTCTGGGGAGGGCCAAGATGAGGCGCTGGGAAGCTTGGGAGCAAGAAACTCGAACAATGGAGTACCAAGTTGAGAATGATCCCAACCGGTTCAGATTTACCAGCCAAACAACATTTGGAAGACGGCACATGAATTCTAGTTCGGAAACATCATTTCATCTGTGGATGGTGAGTGCTTTTACTTGTTTAATCTTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0006952 GO:0008150 GO:0016020 GO:0044464 GO:0050896 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mlo

Protein Analysis

234

Amino Acids

26.58

Weight (kDa)

6.24

Isoelectric Point (pI)

56.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mlo PF03094 8 - 40 6.9e-07 Mlo family
Mlo PF03094 41 - 228 4.4e-56 Mlo family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 261
Acc36I ACCTGC 1 cut(s) 202
AccB1I GGYRCC 1 cut(s) 20
AclWI GGATC 1 cut(s) 578
AcsI RAATTY 2 cut(s) 266, 637
AcyI GRCGYC 2 cut(s) 21, 258
AfaI GTAC 4 cut(s) 333, 414, 488, 569
AfiI CCNNNNNNNGG 1 cut(s) 525
AgeI ACCGGT 1 cut(s) 591
AgsI TTSAA 2 cut(s) 280, 365
AjnI CCWGG 1 cut(s) 201
AluBI AGCT 3 cut(s) 383, 445, 539
AluI AGCT 3 cut(s) 383, 445, 539
Alw21I GWGCWC 1 cut(s) 102
AlwI GGATC 1 cut(s) 578
AlwNI CAGNNNCTG 1 cut(s) 386
AoxI GGCC 1 cut(s) 516
ApeKI GCWGC 4 cut(s) 380, 383, 386, 466
ApoI RAATTY 2 cut(s) 266, 637
ArsI GACNNNNNNTTYG 2 cut(s) 283, 315
AsiGI ACCGGT 1 cut(s) 591
AspLEI GCGC 2 cut(s) 23, 531
AspS9I GGNCC 1 cut(s) 516
AsuHPI GGTGA 3 cut(s) 20, 73, 685
BaeGI GKGCMC 1 cut(s) 52
BanI GGYRCC 1 cut(s) 20
BbsI GAAGAC 1 cut(s) 631
Bbv12I GWGCWC 1 cut(s) 102
BbvI GCAGC 4 cut(s) 370, 373, 392, 453
BccI CCATC 3 cut(s) 113, 117, 664
BceAI ACGGC 1 cut(s) 644
BcgI CGANNNNNNTGC 2 cut(s) 536, 570
BciT130I CCWGG 1 cut(s) 203
BfaI CTAG 1 cut(s) 642
BfmI CTRYAG 1 cut(s) 387
BfoI RGCGCY 2 cut(s) 24, 532
BfuAI ACCTGC 1 cut(s) 202
BisI GCNGC 4 cut(s) 381, 384, 387, 467
BlsI GCNGC 4 cut(s) 382, 385, 388, 468
BmcAI AGTACT 1 cut(s) 333
Bme1390I CCNGG 1 cut(s) 203
BmgT120I GGNCC 1 cut(s) 516
BmiI GGNNCC 1 cut(s) 22
BmrFI CCNGG 1 cut(s) 203
BmsI GCATC 2 cut(s) 117, 367
BpiI GAAGAC 1 cut(s) 631
BsaHI GRCGYC 2 cut(s) 21, 258
BsaJI CCNNGG 2 cut(s) 202, 501
BsaWI WCCGGW 1 cut(s) 591
Bsc4I CCNNNNNNNGG 1 cut(s) 525
Bse118I RCCGGY 2 cut(s) 305, 591
Bse1I ACTGG 1 cut(s) 470
BseBI CCWGG 1 cut(s) 203
BseDI CCNNGG 2 cut(s) 202, 501
BseGI GGATG 3 cut(s) 105, 382, 675
BseLI CCNNNNNNNGG 1 cut(s) 525
BseNI ACTGG 1 cut(s) 470
BseSI GKGCMC 1 cut(s) 52
BseXI GCAGC 4 cut(s) 370, 373, 392, 453
BseYI CCCAGC 1 cut(s) 531
Bsh1285I CGRYCG 1 cut(s) 306
BshFI GGCC 1 cut(s) 518
BshNI GGYRCC 1 cut(s) 20
BshTI ACCGGT 1 cut(s) 591
BsiEI CGRYCG 1 cut(s) 306
BsiHKAI GWGCWC 1 cut(s) 102
BsiSI CCGG 2 cut(s) 306, 592
BslI CCNNNNNNNGG 1 cut(s) 525
BsnI GGCC 1 cut(s) 518
Bsp1286I GDGCHC 2 cut(s) 52, 102
Bsp1407I TGTACA 1 cut(s) 486
Bsp143I GATC 1 cut(s) 583
Bsp19I CCATGG 1 cut(s) 501
BspANI GGCC 1 cut(s) 518
BspHI TCATGA 1 cut(s) 420
BspLI GGNNCC 1 cut(s) 22
BspMAI CTGCAG 1 cut(s) 391
BspMI ACCTGC 1 cut(s) 202
BspPI GGATC 1 cut(s) 578
BspQI GCTCTTC 1 cut(s) 441
BspT107I GGYRCC 1 cut(s) 20
BsrFI RCCGGY 2 cut(s) 305, 591
BsrGI TGTACA 1 cut(s) 486
BsrI ACTGG 1 cut(s) 470
BssAI RCCGGY 2 cut(s) 305, 591
BssECI CCNNGG 2 cut(s) 202, 501
BssMI GATC 1 cut(s) 583
BssNI GRCGYC 2 cut(s) 21, 258
BssT1I CCWWGG 1 cut(s) 501
Bst2UI CCWGG 1 cut(s) 203
Bst4CI ACNGT 3 cut(s) 61, 317, 491
Bst6I CTCTTC 2 cut(s) 360, 441
BstACI GRCGYC 2 cut(s) 21, 258
BstAPI GCANNNNNTGC 1 cut(s) 386
BstAUI TGTACA 1 cut(s) 486
BstDEI CTNAG 1 cut(s) 335
BstDSI CCRYGG 1 cut(s) 501
BstF5I GGATG 3 cut(s) 105, 382, 675
BstH2I RGCGCY 2 cut(s) 24, 532
BstHHI GCGC 2 cut(s) 23, 531
BstKTI GATC 1 cut(s) 586
BstMBI GATC 1 cut(s) 583
BstMCI CGRYCG 1 cut(s) 306
BstMWI GCNNNNNNNGC 2 cut(s) 386, 475
BstNI CCWGG 1 cut(s) 203
BstSCI CCNGG 1 cut(s) 201
BstSFI CTRYAG 1 cut(s) 387
BstSLI GKGCMC 1 cut(s) 52
BstV1I GCAGC 4 cut(s) 370, 373, 392, 453
BstV2I GAAGAC 1 cut(s) 631
BstXI CCANNNNNNTGG 1 cut(s) 508
BsuRI GGCC 1 cut(s) 518
BtgI CCRYGG 1 cut(s) 501
BtsCI GGATG 3 cut(s) 105, 382, 675
BtsI GCAGTG 1 cut(s) 406
BtsIMutI CAGTG 3 cut(s) 354, 406, 496
BveI ACCTGC 1 cut(s) 202
CaiI CAGNNNCTG 1 cut(s) 386
CciI TCATGA 1 cut(s) 420
CfoI GCGC 2 cut(s) 23, 531
Cfr10I RCCGGY 2 cut(s) 305, 591
Cfr13I GGNCC 1 cut(s) 516
Csp6I GTAC 4 cut(s) 332, 413, 487, 568
CspAI ACCGGT 1 cut(s) 591
CviAII CATG 6 cut(s) 45, 214, 222, 421, 502, 634
CviJI RGCY 7 cut(s) 309, 383, 445, 506, 518, 539, 609
CviKI_1 RGCY 7 cut(s) 309, 383, 445, 506, 518, 539, 609
CviQI GTAC 4 cut(s) 332, 413, 487, 568
DdeI CTNAG 1 cut(s) 335
DinI GGCGCC 1 cut(s) 22
DpnI GATC 1 cut(s) 585
DpnII GATC 1 cut(s) 583
Eam1104I CTCTTC 2 cut(s) 360, 441
EarI CTCTTC 2 cut(s) 360, 441
Eco130I CCWWGG 1 cut(s) 501
Eco32I GATATC 1 cut(s) 177
EcoRI GAATTC 1 cut(s) 637
EcoRII CCWGG 1 cut(s) 201
EcoRV GATATC 1 cut(s) 177
EcoT14I CCWWGG 1 cut(s) 501
EcoT22I ATGCAT 1 cut(s) 480
EgeI GGCGCC 1 cut(s) 22
EheI GGCGCC 1 cut(s) 22
ErhI CCWWGG 1 cut(s) 501
FaeI CATG 6 cut(s) 48, 217, 225, 424, 505, 637
FatI CATG 6 cut(s) 44, 213, 221, 420, 501, 633
Fnu4HI GCNGC 4 cut(s) 381, 384, 387, 467
FokI GGATG 3 cut(s) 92, 389, 682
Fsp4HI GCNGC 4 cut(s) 381, 384, 387, 467
FspBI CTAG 1 cut(s) 642
GlaI GCGC 2 cut(s) 22, 530
GluI GCNGC 4 cut(s) 381, 384, 387, 467
GsaI CCCAGC 1 cut(s) 535
HaeII RGCGCY 2 cut(s) 24, 532
HaeIII GGCC 1 cut(s) 518
HapII CCGG 2 cut(s) 306, 592
HhaI GCGC 2 cut(s) 23, 531
Hin1I GRCGYC 2 cut(s) 21, 258
Hin1II CATG 6 cut(s) 48, 217, 225, 424, 505, 637
Hin6I GCGC 2 cut(s) 21, 529
HinP1I GCGC 2 cut(s) 21, 529
HincII GTYRAC 1 cut(s) 121
HindII GTYRAC 1 cut(s) 121
HindIII AAGCTT 1 cut(s) 537
HinfI GANTC 3 cut(s) 11, 170, 392
HpaII CCGG 2 cut(s) 306, 592
HphI GGTGA 3 cut(s) 20, 73, 685
Hpy166II GTNNAC 2 cut(s) 121, 487
Hpy188I TCNGA 3 cut(s) 42, 599, 649
Hpy188III TCNNGA 3 cut(s) 29, 262, 421
Hpy8I GTNNAC 2 cut(s) 121, 487
Hpy99I CGWCG 2 cut(s) 260, 263
HpyAV CCTTC 1 cut(s) 364
HpyCH4III ACNGT 3 cut(s) 61, 317, 491
HpyCH4IV ACGT 1 cut(s) 258
HpyCH4V TGCA 6 cut(s) 189, 352, 380, 389, 399, 478
HpyF10VI GCNNNNNNNGC 2 cut(s) 386, 475
HpyF3I CTNAG 1 cut(s) 335
HpySE526I ACGT 1 cut(s) 258
Hsp92I GRCGYC 2 cut(s) 21, 258
Hsp92II CATG 6 cut(s) 48, 217, 225, 424, 505, 637
HspAI GCGC 2 cut(s) 21, 529
KasI GGCGCC 1 cut(s) 20
Kzo9I GATC 1 cut(s) 583
LguI GCTCTTC 1 cut(s) 441
LmnI GCTCC 1 cut(s) 544
Lsp1109I GCAGC 4 cut(s) 370, 373, 392, 453
LweI GCATC 2 cut(s) 117, 367
MaeI CTAG 1 cut(s) 642
MaeII ACGT 1 cut(s) 258
MaeIII GTNAC 1 cut(s) 144
MalI GATC 1 cut(s) 585
MboI GATC 1 cut(s) 583
MboII GAAGA 6 cut(s) 64, 70, 257, 377, 428, 636
MhlI GDGCHC 2 cut(s) 52, 102
MluCI AATT 2 cut(s) 266, 637
Mly113I GGCGCC 1 cut(s) 21
MmeI TCCRAC 1 cut(s) 65
MnlI CCTC 7 cut(s) 34, 94, 98, 367, 397, 507, 519
Mph1103I ATGCAT 1 cut(s) 480
MseI TTAA 1 cut(s) 692
MspA1I CMGCKG 1 cut(s) 383
MspI CCGG 2 cut(s) 306, 592
MspR9I CCNGG 1 cut(s) 203
MvaI CCWGG 1 cut(s) 203
MwoI GCNNNNNNNGC 2 cut(s) 386, 475
NarI GGCGCC 1 cut(s) 21
NcoI CCATGG 1 cut(s) 501
NdeII GATC 1 cut(s) 583
NlaIII CATG 6 cut(s) 48, 217, 225, 424, 505, 637
NlaIV GGNNCC 1 cut(s) 22
NmuCI GTSAC 1 cut(s) 144
NsiI ATGCAT 1 cut(s) 480
PagI TCATGA 1 cut(s) 420
PciSI GCTCTTC 1 cut(s) 441
PfeI GAWTC 3 cut(s) 11, 170, 392
PflFI GACNNNGTC 1 cut(s) 257
PinAI ACCGGT 1 cut(s) 591
PkrI GCNGC 4 cut(s) 382, 385, 388, 468
PluTI GGCGCC 1 cut(s) 24
Psp6I CCWGG 1 cut(s) 201
PspFI CCCAGC 1 cut(s) 531
PspGI CCWGG 1 cut(s) 201
PspN4I GGNNCC 1 cut(s) 22
PspPI GGNCC 1 cut(s) 516
PstI CTGCAG 1 cut(s) 391
PstNI CAGNNNCTG 1 cut(s) 386
PsyI GACNNNGTC 1 cut(s) 257
PvuII CAGCTG 1 cut(s) 383
RsaI GTAC 4 cut(s) 333, 414, 488, 569
RsaNI GTAC 4 cut(s) 332, 413, 487, 568
SapI GCTCTTC 1 cut(s) 441
SaqAI TTAA 1 cut(s) 692
SatI GCNGC 4 cut(s) 381, 384, 387, 467
Sau3AI GATC 1 cut(s) 583
Sau96I GGNCC 1 cut(s) 516
ScaI AGTACT 1 cut(s) 333
ScrFI CCNGG 1 cut(s) 203
SduI GDGCHC 2 cut(s) 52, 102
SetI ASST 9 cut(s) 10, 86, 196, 204, 261, 299, 385, 447, 541
SfaNI GCATC 2 cut(s) 117, 367
SfcI CTRYAG 1 cut(s) 387
SfoI GGCGCC 1 cut(s) 22
Sse9I AATT 2 cut(s) 266, 637
SspDI GGCGCC 1 cut(s) 20
SspMI CTAG 1 cut(s) 642
StyD4I CCNGG 1 cut(s) 201
StyI CCWWGG 1 cut(s) 501
TaaI ACNGT 3 cut(s) 61, 317, 491
TaiI ACGT 1 cut(s) 261
TaqI TCGA 4 cut(s) 173, 261, 302, 556
TasI AATT 2 cut(s) 266, 637
TatI WGTACW 3 cut(s) 331, 412, 486
TfiI GAWTC 3 cut(s) 11, 170, 392
Tru1I TTAA 1 cut(s) 692
Tru9I TTAA 1 cut(s) 692
TscAI CASTG 3 cut(s) 354, 406, 496
TseFI GTSAC 1 cut(s) 144
TseI GCWGC 4 cut(s) 380, 383, 386, 466
Tsp45I GTSAC 1 cut(s) 144
TspDTI ATGAA 6 cut(s) 58, 64, 342, 409, 650, 650
TspRI CASTG 3 cut(s) 354, 406, 496
Tth111I GACNNNGTC 1 cut(s) 257
XapI RAATTY 2 cut(s) 266, 637
XcmI CCANNNNNNNNNTGG 1 cut(s) 617
XspI CTAG 1 cut(s) 642
ZraI GACGTC 1 cut(s) 259
ZrmI AGTACT 1 cut(s) 333
Zsp2I ATGCAT 1 cut(s) 480
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.