Rorug05G0327200

MLO-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0002520
Physical Location & Seq
Forward (+)
39365014 .. 39365445
432 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0327200.1

Sequence Viewer

Length: 432 bp
ATGGCTTCTTGCATAAATATCAATTTACTTGCAGTGCTGCTTTTCTTGTTCCTAACTAGTTGTGCAGCATCTGGCGGGCCATTTAGAAGAGTTCATGTTACAATTGCTAATGAGTTGGGTGGAGACTTGGTTCTCTTAGCTCATTGCTGGTCCTTCGATGATGATCTTGGTGTCCGTACCCTCCCCCTTCATGCCTCCTTTGAATTTAGTTTTCGAATTGGTTTTTGGCACAATACAGCATTCAAGTGCGAATTTAGTTGGGTAATTAAAGGTTTTCAGACTTTTATACACACAGCAGTTATATTCGATGCCAACAGAGATAAGTGTCAAAATTGTTCATGGATTATTACCCCAAATGGGCCATGCATGCACAACTTCGATGCCCATAATTTGAATTGTTATACTTGGCCAACTTCGATGCCCATAGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0006952 GO:0008150 GO:0016020 GO:0044464 GO:0050896 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mlo

Protein Analysis

143

Amino Acids

16.13

Weight (kDa)

6.34

Isoelectric Point (pI)

36.68

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Self-incomp_S1 PF05938 32 - 136 3.7e-22 Plant self-incompatibility protein S1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 75
AcoI YGGCCR 1 cut(s) 407
AcsI RAATTY 2 cut(s) 203, 251
AfaI GTAC 1 cut(s) 178
AfiI CCNNNNNNNGG 1 cut(s) 357
AgsI TTSAA 3 cut(s) 203, 244, 394
AhlI ACTAGT 1 cut(s) 56
AjuI GAANNNNNNNTTGG 2 cut(s) 208, 240
AloI GAACNNNNNNTCC 2 cut(s) 114, 146
AluBI AGCT 1 cut(s) 140
AluI AGCT 1 cut(s) 140
Alw26I GTCTC 1 cut(s) 117
AlwNI CAGNNNCTG 1 cut(s) 71
AoxI GGCC 3 cut(s) 77, 359, 407
ApeKI GCWGC 2 cut(s) 37, 65
ApoI RAATTY 2 cut(s) 203, 251
AspS9I GGNCC 3 cut(s) 77, 150, 359
AsuII TTCGAA 1 cut(s) 214
AvaII GGWCC 1 cut(s) 150
BalI TGGCCA 1 cut(s) 409
BbvI GCAGC 2 cut(s) 24, 77
BcoDI GTCTC 1 cut(s) 117
BcuI ACTAGT 1 cut(s) 56
BfaI CTAG 1 cut(s) 57
BisI GCNGC 2 cut(s) 38, 66
BlsI GCNGC 2 cut(s) 39, 67
Bme18I GGWCC 1 cut(s) 150
BmgT120I GGNCC 3 cut(s) 77, 150, 359
BmsI GCATC 4 cut(s) 77, 298, 370, 408
Bpu14I TTCGAA 1 cut(s) 214
BsaBI GATNNNNATC 1 cut(s) 162
BsaXI ACNNNNNCTCC 2 cut(s) 114, 144
Bsc4I CCNNNNNNNGG 1 cut(s) 357
Bse3DI GCAATG 1 cut(s) 142
Bse8I GATNNNNATC 1 cut(s) 162
BseJI GATNNNNATC 1 cut(s) 162
BseLI CCNNNNNNNGG 1 cut(s) 357
BseMI GCAATG 1 cut(s) 142
BseXI GCAGC 2 cut(s) 24, 77
BsgI GTGCAG 1 cut(s) 84
BshFI GGCC 3 cut(s) 79, 361, 409
BslI CCNNNNNNNGG 1 cut(s) 357
BsmAI GTCTC 1 cut(s) 117
BsmI GAATGC 1 cut(s) 239
BsnI GGCC 3 cut(s) 79, 361, 409
Bsp119I TTCGAA 1 cut(s) 214
Bsp143I GATC 1 cut(s) 163
BspACI CCGC 1 cut(s) 75
BspANI GGCC 3 cut(s) 79, 361, 409
BspT104I TTCGAA 1 cut(s) 214
BsrDI GCAATG 1 cut(s) 142
BssMI GATC 1 cut(s) 163
Bst6I CTCTTC 1 cut(s) 82
BstBI TTCGAA 1 cut(s) 214
BstC8I GCNNGC 2 cut(s) 77, 368
BstDEI CTNAG 1 cut(s) 136
BstKTI GATC 1 cut(s) 166
BstMAI GTCTC 1 cut(s) 117
BstMBI GATC 1 cut(s) 163
BstMWI GCNNNNNNNGC 1 cut(s) 367
BstNSI RCATGY 1 cut(s) 370
BstV1I GCAGC 2 cut(s) 24, 77
BsuRI GGCC 3 cut(s) 79, 361, 409
BtsI GCAGTG 1 cut(s) 39
BtsIMutI CAGTG 1 cut(s) 39
Cac8I GCNNGC 2 cut(s) 77, 368
CaiI CAGNNNCTG 1 cut(s) 71
Cfr13I GGNCC 3 cut(s) 77, 150, 359
Csp6I GTAC 1 cut(s) 177
CviAII CATG 5 cut(s) 95, 191, 339, 363, 367
CviJI RGCY 5 cut(s) 5, 79, 140, 361, 409
CviKI_1 RGCY 5 cut(s) 5, 79, 140, 361, 409
CviQI GTAC 1 cut(s) 177
DdeI CTNAG 1 cut(s) 136
DpnI GATC 1 cut(s) 165
DpnII GATC 1 cut(s) 163
EaeI YGGCCR 1 cut(s) 407
Eam1104I CTCTTC 1 cut(s) 82
EarI CTCTTC 1 cut(s) 82
Eco47I GGWCC 1 cut(s) 150
EcoT22I ATGCAT 1 cut(s) 368
FaeI CATG 5 cut(s) 98, 194, 342, 366, 370
FatI CATG 5 cut(s) 94, 190, 338, 362, 366
FauI CCCGC 1 cut(s) 68
Fnu4HI GCNGC 2 cut(s) 38, 66
Fsp4HI GCNGC 2 cut(s) 38, 66
FspBI CTAG 1 cut(s) 57
GluI GCNGC 2 cut(s) 38, 66
HaeIII GGCC 3 cut(s) 79, 361, 409
Hin1II CATG 5 cut(s) 98, 194, 342, 366, 370
Hpy188I TCNGA 1 cut(s) 279
HpyAV CCTTC 2 cut(s) 163, 197
HpyCH4V TGCA 5 cut(s) 12, 32, 65, 366, 370
HpyF10VI GCNNNNNNNGC 1 cut(s) 367
HpyF3I CTNAG 1 cut(s) 136
Hsp92II CATG 5 cut(s) 98, 194, 342, 366, 370
Kzo9I GATC 1 cut(s) 163
LpnPI CCDG 2 cut(s) 57, 133
Lsp1109I GCAGC 2 cut(s) 24, 77
LweI GCATC 4 cut(s) 77, 298, 370, 408
MaeI CTAG 1 cut(s) 57
MaeIII GTNAC 1 cut(s) 97
MalI GATC 1 cut(s) 165
MboI GATC 1 cut(s) 163
MboII GAAGA 1 cut(s) 99
MfeI CAATTG 1 cut(s) 102
MlsI TGGCCA 1 cut(s) 409
MluCI AATT 9 cut(s) 22, 102, 203, 216, 251, 264, 331, 388, 394
MluNI TGGCCA 1 cut(s) 409
MnlI CCTC 2 cut(s) 191, 205
Mox20I TGGCCA 1 cut(s) 409
Mph1103I ATGCAT 1 cut(s) 368
MscI TGGCCA 1 cut(s) 409
MseI TTAA 1 cut(s) 267
MslI CAYNNNNRTG 1 cut(s) 244
Msp20I TGGCCA 1 cut(s) 409
MunI CAATTG 1 cut(s) 102
Mva1269I GAATGC 1 cut(s) 239
MwoI GCNNNNNNNGC 1 cut(s) 367
NdeII GATC 1 cut(s) 163
NlaIII CATG 5 cut(s) 98, 194, 342, 366, 370
NsiI ATGCAT 1 cut(s) 368
NspI RCATGY 1 cut(s) 370
NspV TTCGAA 1 cut(s) 214
PaeI GCATGC 1 cut(s) 370
PctI GAATGC 1 cut(s) 239
PkrI GCNGC 2 cut(s) 39, 67
PspPI GGNCC 3 cut(s) 77, 150, 359
PstNI CAGNNNCTG 1 cut(s) 71
RsaI GTAC 1 cut(s) 178
RsaNI GTAC 1 cut(s) 177
RseI CAYNNNNRTG 1 cut(s) 244
SaqAI TTAA 1 cut(s) 267
SatI GCNGC 2 cut(s) 38, 66
Sau3AI GATC 1 cut(s) 163
Sau96I GGNCC 3 cut(s) 77, 150, 359
SetI ASST 2 cut(s) 142, 274
SfaNI GCATC 4 cut(s) 77, 298, 370, 408
SfuI TTCGAA 1 cut(s) 214
SinI GGWCC 1 cut(s) 150
SmiMI CAYNNNNRTG 1 cut(s) 244
SpeI ACTAGT 1 cut(s) 56
SphI GCATGC 1 cut(s) 370
Sse9I AATT 9 cut(s) 22, 102, 203, 216, 251, 264, 331, 388, 394
SsiI CCGC 1 cut(s) 75
SspMI CTAG 1 cut(s) 57
TaqI TCGA 5 cut(s) 156, 214, 306, 378, 416
TasI AATT 9 cut(s) 22, 102, 203, 216, 251, 264, 331, 388, 394
Tru1I TTAA 1 cut(s) 267
Tru9I TTAA 1 cut(s) 267
TscAI CASTG 1 cut(s) 39
TseI GCWGC 2 cut(s) 37, 65
TspDTI ATGAA 3 cut(s) 83, 179, 327
TspGWI ACGGA 1 cut(s) 164
TspRI CASTG 1 cut(s) 39
VpaK11BI GGWCC 1 cut(s) 150
XapI RAATTY 2 cut(s) 203, 251
XceI RCATGY 1 cut(s) 370
XspI CTAG 1 cut(s) 57
Zsp2I ATGCAT 1 cut(s) 368
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.