Rh3CG163900

MLO-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3C
OG0002520
Physical Location & Seq
Reverse (-)
13832523 .. 13836570
4048 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3CG163900.1

Sequence Viewer

Length: 1029 bp
ATGGCAGTAGCCGTTGAAGGCACCACTTTGGAATACACACCGACATGGGCTCTTGCCACTGTTTGCTTCTTCTTCATCTCTGTCTCCATCTTGATTGAACACTTAATCCACCTCCTTGTGACATGGCTCAAAAACCATCGAAAAACCGCCTTAACTGATGCGGTGGAGAAGCTCAAAGCAGAGTTAATGCTTCTGGGATTTGTGTCACTTTTATTGGCGGTGACCCAAGATAGGATAGCGAAAATTTGTATACCTGCAAAGATAGGAGATATAATGCTTCCCTGTCGTAAAGCCGTGAATGAGACAGAAGTGAAAACCGTTGAGCACTATGTGAACAAATTTGCAAGAAACCTTAATTCTTCTGGGGTACATGTCACGTTTGACCAAATGCTCAGGCAAACGCAGAGGATACTCGCCGAGGAGGAGGCAGCAGCTGTTGAAGATTATTGCGGTGATGAAAAGGTTTCGTTGGTGACGAAAAACGCACTTCATCAGCTGCACTTGCTGATCTTTGCGCTTGCGGTTATGCATATTGTGTATAGTGTTCTAACCATGGCTTTGGGCAGGGCTAAGATGAGGCGCTGGGAAGCATGGGAGGAAGAAACACGAACGATGGAGTACCAAGTTGCCAATGACCCCAATCGATTCAGACTTACAAGACAAACAACATTTGGAAAAAGGCACATGAATTCGTGCACAGAGACTTCACTTCATCTATGGCTGATGGGTTCACAATTCAAGGGTAAGATACTAGAAGATAACATGGCTGGCATTCTAAAGCAATGGCACAGTGATGTGAGGCGGAGAAGGAAGAGGGAAGAACAAGAATCACAATCTGCACGCACGTCTTTTTCCATGGAATGGAGCTCTGTGAGGCACAGCATTTCTTCTTATATGAGACCAAGCACTTCCAGACGAGGTGAAAATGCCGATTTTTCTAACAGATGGGATAACAAGGACCAGACAGTAGAATTAGAAGAAAGCTCCTCATCAAGGGGTCACAGAAGAAATAGTGATTATGGGACATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0006952 GO:0008150 GO:0016020 GO:0044464 GO:0050896 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mlo

Protein Analysis

342

Amino Acids

39.41

Weight (kDa)

8.89

Isoelectric Point (pI)

56.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mlo PF03094 8 - 241 7.4e-79 Mlo family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 262
AccB1I GGYRCC 1 cut(s) 20
AccB7I CCANNNNNTGG 1 cut(s) 861
AccI GTMKAC 1 cut(s) 250
AciI CCGC 6 cut(s) 147, 161, 218, 450, 521, 802
AcsI RAATTY 3 cut(s) 243, 338, 688
AdeI CACNNNGTG 1 cut(s) 331
AfaI GTAC 2 cut(s) 369, 620
AfiI CCNNNNNNNGG 3 cut(s) 231, 861, 993
AflIII ACRYGT 1 cut(s) 370
AgsI TTSAA 4 cut(s) 17, 98, 440, 739
AjiI CACGTC 1 cut(s) 846
AloI GAACNNNNNNTCC 2 cut(s) 90, 122
AluBI AGCT 5 cut(s) 172, 434, 496, 867, 984
AluI AGCT 5 cut(s) 172, 434, 496, 867, 984
Alw21I GWGCWC 3 cut(s) 327, 698, 869
Alw26I GTCTC 4 cut(s) 88, 296, 695, 892
Alw44I GTGCAC 1 cut(s) 694
AlwNI CAGNNNCTG 1 cut(s) 434
ApaLI GTGCAC 1 cut(s) 694
ApeKI GCWGC 3 cut(s) 428, 431, 496
ApoI RAATTY 3 cut(s) 243, 338, 688
AspLEI GCGC 2 cut(s) 517, 582
AspS9I GGNCC 1 cut(s) 958
AsuHPI GGTGA 4 cut(s) 232, 464, 484, 932
AvaII GGWCC 1 cut(s) 958
BaeGI GKGCMC 1 cut(s) 698
BanI GGYRCC 1 cut(s) 20
BanII GRGCYC 2 cut(s) 52, 869
Bbv12I GWGCWC 3 cut(s) 327, 698, 869
BbvI GCAGC 3 cut(s) 440, 443, 483
BccI CCATC 5 cut(s) 95, 144, 607, 718, 939
BceAI ACGGC 1 cut(s) 278
BciVI GTATCC 1 cut(s) 402
BcoDI GTCTC 4 cut(s) 88, 296, 695, 892
BfaI CTAG 1 cut(s) 752
BfoI RGCGCY 1 cut(s) 583
BfuAI ACCTGC 1 cut(s) 262
BfuI GTATCC 1 cut(s) 402
BisI GCNGC 3 cut(s) 429, 432, 497
BlsI GCNGC 3 cut(s) 430, 433, 498
Bme18I GGWCC 1 cut(s) 958
BmgBI CACGTC 1 cut(s) 846
BmgT120I GGNCC 1 cut(s) 958
BmiI GGNNCC 1 cut(s) 22
BmsI GCATC 1 cut(s) 148
Bpu10I CCTNAGC 1 cut(s) 392
Bsa29I ATCGAT 1 cut(s) 643
BsaI GGTCTC 1 cut(s) 892
BsaJI CCNNGG 3 cut(s) 417, 552, 855
Bsc4I CCNNNNNNNGG 3 cut(s) 231, 861, 993
Bse3DI GCAATG 1 cut(s) 788
BseCI ATCGAT 1 cut(s) 643
BseDI CCNNGG 3 cut(s) 417, 552, 855
BseLI CCNNNNNNNGG 3 cut(s) 231, 861, 993
BseMI GCAATG 1 cut(s) 788
BseMII CTCAG 1 cut(s) 406
BseRI GAGGAG 3 cut(s) 434, 437, 976
BseSI GKGCMC 1 cut(s) 698
BseXI GCAGC 3 cut(s) 440, 443, 483
BseYI CCCAGC 1 cut(s) 582
BsgI GTGCAG 2 cut(s) 482, 822
BshNI GGYRCC 1 cut(s) 20
BshVI ATCGAT 1 cut(s) 643
BsiHKAI GWGCWC 3 cut(s) 327, 698, 869
BslI CCNNNNNNNGG 3 cut(s) 231, 861, 993
BsmAI GTCTC 4 cut(s) 88, 296, 695, 892
BsmI GAATGC 1 cut(s) 771
Bso31I GGTCTC 1 cut(s) 892
Bsp1286I GDGCHC 4 cut(s) 52, 327, 698, 869
Bsp143I GATC 1 cut(s) 507
Bsp19I CCATGG 2 cut(s) 552, 855
BspACI CCGC 6 cut(s) 147, 161, 218, 450, 521, 802
BspCNI CTCAG 1 cut(s) 405
BspDI ATCGAT 1 cut(s) 643
BspLI GGNNCC 1 cut(s) 22
BspMI ACCTGC 1 cut(s) 262
BspT107I GGYRCC 1 cut(s) 20
BspTNI GGTCTC 1 cut(s) 892
BsrDI GCAATG 1 cut(s) 788
BssECI CCNNGG 3 cut(s) 417, 552, 855
BssMI GATC 1 cut(s) 507
BssNAI GTATAC 1 cut(s) 251
BssT1I CCWWGG 2 cut(s) 552, 855
Bst1107I GTATAC 1 cut(s) 251
Bst4CI ACNGT 4 cut(s) 61, 319, 791, 967
Bst6I CTCTTC 1 cut(s) 806
BstC8I GCNNGC 3 cut(s) 519, 769, 841
BstDEI CTNAG 2 cut(s) 392, 570
BstDSI CCRYGG 2 cut(s) 552, 855
BstEII GGTNACC 1 cut(s) 220
BstENI CCTNNNNNAGG 1 cut(s) 991
BstH2I RGCGCY 1 cut(s) 583
BstHHI GCGC 2 cut(s) 517, 582
BstKTI GATC 1 cut(s) 510
BstMAI GTCTC 4 cut(s) 88, 296, 695, 892
BstMBI GATC 1 cut(s) 507
BstMWI GCNNNNNNNGC 1 cut(s) 502
BstNSI RCATGY 1 cut(s) 374
BstPI GGTNACC 1 cut(s) 220
BstSLI GKGCMC 1 cut(s) 698
BstV1I GCAGC 3 cut(s) 440, 443, 483
BstXI CCANNNNNNTGG 1 cut(s) 559
BstZ17I GTATAC 1 cut(s) 251
Bsu15I ATCGAT 1 cut(s) 643
BsuI GTATCC 1 cut(s) 402
BsuTUI ATCGAT 1 cut(s) 643
BtgI CCRYGG 2 cut(s) 552, 855
BtrI CACGTC 1 cut(s) 846
BtsIMutI CAGTG 2 cut(s) 57, 796
BveI ACCTGC 1 cut(s) 262
Cac8I GCNNGC 3 cut(s) 519, 769, 841
CaiI CAGNNNCTG 1 cut(s) 434
CfoI GCGC 2 cut(s) 517, 582
Cfr13I GGNCC 1 cut(s) 958
ClaI ATCGAT 1 cut(s) 643
Csp6I GTAC 2 cut(s) 368, 619
CviAII CATG 9 cut(s) 45, 123, 371, 553, 591, 685, 763, 856, 1026
CviQI GTAC 2 cut(s) 368, 619
DdeI CTNAG 2 cut(s) 392, 570
DpnI GATC 1 cut(s) 509
DpnII GATC 1 cut(s) 507
DraIII CACNNNGTG 1 cut(s) 331
Eam1104I CTCTTC 1 cut(s) 806
EarI CTCTTC 1 cut(s) 806
EciI GGCGGA 1 cut(s) 817
Ecl136II GAGCTC 1 cut(s) 867
Eco130I CCWWGG 2 cut(s) 552, 855
Eco24I GRGCYC 2 cut(s) 52, 869
Eco31I GGTCTC 1 cut(s) 892
Eco47I GGWCC 1 cut(s) 958
Eco53kI GAGCTC 1 cut(s) 867
Eco91I GGTNACC 1 cut(s) 220
EcoICRI GAGCTC 1 cut(s) 867
EcoNI CCTNNNNNAGG 1 cut(s) 991
EcoO65I GGTNACC 1 cut(s) 220
EcoRI GAATTC 1 cut(s) 688
EcoT14I CCWWGG 2 cut(s) 552, 855
EcoT22I ATGCAT 1 cut(s) 531
EcoT38I GRGCYC 2 cut(s) 52, 869
ErhI CCWWGG 2 cut(s) 552, 855
FaeI CATG 9 cut(s) 48, 126, 374, 556, 594, 688, 766, 859, 1029
FatI CATG 9 cut(s) 44, 122, 370, 552, 590, 684, 762, 855, 1025
FblI GTMKAC 1 cut(s) 250
Fnu4HI GCNGC 3 cut(s) 429, 432, 497
FriOI GRGCYC 2 cut(s) 52, 869
Fsp4HI GCNGC 3 cut(s) 429, 432, 497
FspBI CTAG 1 cut(s) 752
GlaI GCGC 2 cut(s) 516, 581
GluI GCNGC 3 cut(s) 429, 432, 497
GsaI CCCAGC 1 cut(s) 586
HaeII RGCGCY 1 cut(s) 583
HhaI GCGC 2 cut(s) 517, 582
Hin1II CATG 9 cut(s) 48, 126, 374, 556, 594, 688, 766, 859, 1029
Hin6I GCGC 2 cut(s) 515, 580
HinP1I GCGC 2 cut(s) 515, 580
HinfI GANTC 2 cut(s) 645, 827
HphI GGTGA 4 cut(s) 232, 464, 484, 932
Hpy166II GTNNAC 4 cut(s) 251, 334, 696, 731
Hpy188I TCNGA 1 cut(s) 650
Hpy188III TCNNGA 2 cut(s) 91, 912
Hpy8I GTNNAC 4 cut(s) 251, 334, 696, 731
HpyAV CCTTC 2 cut(s) 11, 801
HpyCH4III ACNGT 4 cut(s) 61, 319, 791, 967
HpyCH4IV ACGT 2 cut(s) 377, 845
HpyCH4V TGCA 6 cut(s) 257, 344, 499, 529, 696, 839
HpyF10VI GCNNNNNNNGC 1 cut(s) 502
HpyF3I CTNAG 2 cut(s) 392, 570
HpySE526I ACGT 2 cut(s) 377, 845
Hsp92II CATG 9 cut(s) 48, 126, 374, 556, 594, 688, 766, 859, 1029
HspAI GCGC 2 cut(s) 515, 580
Kzo9I GATC 1 cut(s) 507
LmnI GCTCC 2 cut(s) 864, 989
Lsp1109I GCAGC 3 cut(s) 440, 443, 483
LweI GCATC 1 cut(s) 148
MaeI CTAG 1 cut(s) 752
MaeII ACGT 2 cut(s) 377, 845
MaeIII GTNAC 6 cut(s) 118, 204, 220, 373, 472, 998
MalI GATC 1 cut(s) 509
MboI GATC 1 cut(s) 507
MhlI GDGCHC 4 cut(s) 52, 327, 698, 869
MluCI AATT 6 cut(s) 243, 338, 355, 688, 734, 971
Mph1103I ATGCAT 1 cut(s) 531
MseI TTAA 4 cut(s) 104, 152, 185, 354
MslI CAYNNNNRTG 2 cut(s) 43, 792
MspA1I CMGCKG 2 cut(s) 434, 496
Mva1269I GAATGC 1 cut(s) 771
MwoI GCNNNNNNNGC 1 cut(s) 502
NcoI CCATGG 2 cut(s) 552, 855
NdeII GATC 1 cut(s) 507
NlaIII CATG 9 cut(s) 48, 126, 374, 556, 594, 688, 766, 859, 1029
NlaIV GGNNCC 1 cut(s) 22
NmeAIII GCCGAG 1 cut(s) 442
NmuCI GTSAC 6 cut(s) 118, 204, 220, 373, 472, 998
NsiI ATGCAT 1 cut(s) 531
NspI RCATGY 1 cut(s) 374
PciI ACATGT 1 cut(s) 370
PcsI WCGNNNNNNNCGW 1 cut(s) 473
PctI GAATGC 1 cut(s) 771
PfeI GAWTC 2 cut(s) 645, 827
PflMI CCANNNNNTGG 1 cut(s) 861
PkrI GCNGC 3 cut(s) 430, 433, 498
PscI ACATGT 1 cut(s) 370
Psp124BI GAGCTC 1 cut(s) 869
PspEI GGTNACC 1 cut(s) 220
PspFI CCCAGC 1 cut(s) 582
PspN4I GGNNCC 1 cut(s) 22
PspPI GGNCC 1 cut(s) 958
PstNI CAGNNNCTG 1 cut(s) 434
PvuII CAGCTG 2 cut(s) 434, 496
RsaI GTAC 2 cut(s) 369, 620
RsaNI GTAC 2 cut(s) 368, 619
RseI CAYNNNNRTG 2 cut(s) 43, 792
SacI GAGCTC 1 cut(s) 869
SaqAI TTAA 4 cut(s) 104, 152, 185, 354
SatI GCNGC 3 cut(s) 429, 432, 497
Sau3AI GATC 1 cut(s) 507
Sau96I GGNCC 1 cut(s) 958
SduI GDGCHC 4 cut(s) 52, 327, 698, 869
SfaNI GCATC 1 cut(s) 148
SinI GGWCC 1 cut(s) 958
SmiMI CAYNNNNRTG 2 cut(s) 43, 792
Sse9I AATT 6 cut(s) 243, 338, 355, 688, 734, 971
SsiI CCGC 6 cut(s) 147, 161, 218, 450, 521, 802
SspMI CTAG 1 cut(s) 752
SstI GAGCTC 1 cut(s) 869
StyI CCWWGG 2 cut(s) 552, 855
TaaI ACNGT 4 cut(s) 61, 319, 791, 967
TaiI ACGT 2 cut(s) 380, 848
TaqI TCGA 2 cut(s) 139, 643
TasI AATT 6 cut(s) 243, 338, 355, 688, 734, 971
TfiI GAWTC 2 cut(s) 645, 827
Tru1I TTAA 4 cut(s) 104, 152, 185, 354
Tru9I TTAA 4 cut(s) 104, 152, 185, 354
TscAI CASTG 2 cut(s) 64, 796
TseFI GTSAC 6 cut(s) 118, 204, 220, 373, 472, 998
TseI GCWGC 3 cut(s) 428, 431, 496
Tsp45I GTSAC 6 cut(s) 118, 204, 220, 373, 472, 998
TspDTI ATGAA 5 cut(s) 64, 471, 479, 701, 701
TspRI CASTG 2 cut(s) 64, 796
Van91I CCANNNNNTGG 1 cut(s) 861
VneI GTGCAC 1 cut(s) 694
VpaK11BI GGWCC 1 cut(s) 958
XagI CCTNNNNNAGG 1 cut(s) 991
XapI RAATTY 3 cut(s) 243, 338, 688
XceI RCATGY 1 cut(s) 374
XmiI GTMKAC 1 cut(s) 250
XspI CTAG 1 cut(s) 752
Zsp2I ATGCAT 1 cut(s) 531
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.