MD03G1115800.v1.1

MLO-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0002520
Physical Location & Seq
Forward (+)
10239044 .. 10245100
6057 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1115800.v1.1.491

Sequence Viewer

Length: 1647 bp
ATGGCTTCTGGAGGATCAGCATCCAGCCGTTCACTTCAAGACACACCCACCTGGGCTTTGGCAGCAGTTTGCTTTGTGTTCATATTTCTCTCTCTATTTATAGAGCACTTGATCCATCTCATTAGCAAATGGCTCAAAACTCACAGAAAGACAGCCTTGTTCGAGGCCGTAGAGAAGCTCAAATCAGTTCTTATACTCCTGGGATTTATGTCACTAATTTTGACAGTATCACAAAGATCCATATCCAAAATATGCATACCCACAAAAGTTGCATATACCATGCTTCCCTGCAGAAAAAAAGCACCAACAAAAACCACCAAAGCACTTGGTTTTGAGCAAATATCAGTAGAAAGAATGCTTGCAGCAGCTGATACCAGTGAGGATCACTGTGAGACCCAGGGAAAGACCTCGTTTATATCTCAAGGGGGCCTCAATCAGCTCAACAACTTCATCTTTGTTTTGGCAGTTATGCAGATTGTGTATAGTGTTCTCACCATGGCTTTAGGAAGGGCCAAGATGAGGCGTTGGGAAGCATGGGAAAAAGAAACTCAGACAGTGGAATACCAAGTGGCAAATGATCCTAATCGGTTTAGGTTTACAAGGCAGACAACATTTGCTCTTAGACATATGAGTTCTTGTACAGGAAGTGGAACATCTGTTGGACTGTGGATTAGGTGCTTCTTCCGGCAGTTTTTACATTCAGTGGAGAAAGTTGACTACCTCACTCTGCGTCATGGTTTCGTTTCGACTCATTTTCCAACAAACAACAATGCATTCGATTTCCAAAAGTATATACAACGATCACTGGAGGATGATTTTAGAGCTGTAGTCGGAATCAGCCCTTTCATGTGGTTTATAGTCGTCATCTTCATCCTCCTCGACGTGCATGGTTGGCAGGTGTATCTCTGGGTATCATTTGTGCCACTAATTATAGTGCTAGTTCTTGGAACCAAACTTGAAGTAGTAGTGGCAAACATGGCTCATCAACTTCATGACCAGCATAATGTGATTCAAGGAGCTCCTCTGGTGCAACCAAAGGACAGTCACTTCTGGTTCAATCAACCGCGGTTCGTCTTGATTCTTGTACATCTCACATTGTTTATGAATGCTTTTGAACTTGCTTTCTTCATATGGGTCACGATACAATTTGGGATACACTCCTGCTACCATGAACACATACAGATTACAATTGTACGAGTGGTTTTAGCGGTGACGGTCCAAGTGATGTGCAGTTACATTACTCTTCCTCTCTATGCTCTAGTGACCCAGATGGGATCAAATTTCAAGAGTGCAGCAGTGCTTGAAGAGCAAACAACATATGTTATAAAGCAGTGGCACGCTGATGTAAAGAGAAAGAGGAAAAATAAATCACACTATTCACAATCTGGTCATGACGAAGATTCATCTACGGTGGGAAGCAGCTGGGAAATCATGAATTCCCCAGATTTTTCTTCTCATCGTCCCCAACTTTCTTTCGCCGAAATGAACCACAGTCACCCTAGAGAAACTGAGAATTTTTATGGTAACGAAGAAATTGTTGAAGATCATCAAGAAAGGGTTGGACAAAATGAAATTGAACTAGCTAGTAATGCTTCATCTACTGAGGTACATATAGGAATGCCGGAGGTCACCAGAACACAAACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0006952 GO:0008150 GO:0016020 GO:0044464 GO:0050896 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mlo

Protein Analysis

549

Amino Acids

62.65

Weight (kDa)

8.08

Isoelectric Point (pI)

39.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mlo PF03094 10 - 464 4.3e-192 Mlo family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1325
AarI CACCTGC 1 cut(s) 886
Acc36I ACCTGC 1 cut(s) 886
AccII CGCG 1 cut(s) 1066
AciI CCGC 3 cut(s) 1064, 1066, 1208
AclWI GGATC 6 cut(s) 22, 106, 231, 390, 572, 1282
AcsI RAATTY 3 cut(s) 1279, 1435, 1513
AfaI GTAC 4 cut(s) 640, 1086, 1194, 1608
AfiI CCNNNNNNNGG 1 cut(s) 519
AgsI TTSAA 9 cut(s) 38, 959, 1013, 1057, 1115, 1285, 1304, 1541, 1577
AjiI CACGTC 1 cut(s) 883
AjnI CCWGG 3 cut(s) 50, 198, 396
AluBI AGCT 7 cut(s) 178, 368, 439, 824, 1019, 1422, 1583
AluI AGCT 7 cut(s) 178, 368, 439, 824, 1019, 1422, 1583
Alw21I GWGCWC 2 cut(s) 108, 1021
Alw26I GTCTC 1 cut(s) 386
AlwI GGATC 6 cut(s) 22, 106, 231, 390, 572, 1282
AlwNI CAGNNNCTG 1 cut(s) 368
AoxI GGCC 3 cut(s) 165, 427, 510
ApeKI GCWGC 5 cut(s) 62, 362, 365, 1292, 1419
ApoI RAATTY 3 cut(s) 1279, 1435, 1513
AspS9I GGNCC 3 cut(s) 427, 510, 1216
AsuHPI GGTGA 4 cut(s) 484, 1222, 1487, 1621
AvaII GGWCC 1 cut(s) 1216
BanII GRGCYC 1 cut(s) 1021
Bbv12I GWGCWC 2 cut(s) 108, 1021
BbvI GCAGC 5 cut(s) 74, 374, 377, 1304, 1431
BccI CCATC 2 cut(s) 123, 1264
BceAI ACGGC 2 cut(s) 12, 152
BciT130I CCWGG 3 cut(s) 52, 200, 398
BciVI GTATCC 1 cut(s) 1146
BcoDI GTCTC 1 cut(s) 386
BfaI CTAG 5 cut(s) 938, 1259, 1500, 1580, 1584
BfmI CTRYAG 2 cut(s) 289, 825
BfuAI ACCTGC 1 cut(s) 886
BfuI GTATCC 1 cut(s) 1146
BisI GCNGC 5 cut(s) 63, 363, 366, 1293, 1420
BlsI GCNGC 5 cut(s) 64, 364, 367, 1294, 1421
Bme1390I CCNGG 3 cut(s) 52, 200, 398
Bme18I GGWCC 1 cut(s) 1216
BmgBI CACGTC 1 cut(s) 883
BmgT120I GGNCC 3 cut(s) 427, 510, 1216
BmiI GGNNCC 2 cut(s) 428, 949
BmrFI CCNGG 3 cut(s) 52, 200, 398
BmsI GCATC 1 cut(s) 29
BpmI CTGGAG 2 cut(s) 30, 827
BpuEI CTTGAG 1 cut(s) 405
BsaBI GATNNNNATC 3 cut(s) 19, 241, 582
BsaI GGTCTC 1 cut(s) 386
BsaJI CCNNGG 6 cut(s) 51, 199, 396, 397, 495, 1064
Bsc4I CCNNNNNNNGG 1 cut(s) 519
Bse1I ACTGG 2 cut(s) 375, 810
Bse8I GATNNNNATC 3 cut(s) 19, 241, 582
BseBI CCWGG 3 cut(s) 52, 200, 398
BseDI CCNNGG 6 cut(s) 51, 199, 396, 397, 495, 1064
BseGI GGATG 3 cut(s) 20, 817, 870
BseJI GATNNNNATC 3 cut(s) 19, 241, 582
BseLI CCNNNNNNNGG 1 cut(s) 519
BseMII CTCAG 3 cut(s) 563, 1500, 1593
BseNI ACTGG 2 cut(s) 375, 810
BseRI GAGGAG 2 cut(s) 866, 1011
BseXI GCAGC 5 cut(s) 74, 374, 377, 1304, 1431
BseYI CCCAGC 1 cut(s) 1422
BsgI GTGCAG 2 cut(s) 1249, 1311
Bsh1236I CGCG 1 cut(s) 1066
BshFI GGCC 3 cut(s) 167, 429, 512
BsiHKAI GWGCWC 2 cut(s) 108, 1021
BsiSI CCGG 2 cut(s) 685, 1622
BslFI GGGAC 1 cut(s) 1446
BslI CCNNNNNNNGG 1 cut(s) 519
BsmAI GTCTC 1 cut(s) 386
BsmFI GGGAC 1 cut(s) 1446
BsmI GAATGC 4 cut(s) 360, 773, 1111, 1623
BsnI GGCC 3 cut(s) 167, 429, 512
Bso31I GGTCTC 1 cut(s) 386
Bsp1286I GDGCHC 2 cut(s) 108, 1021
Bsp1407I TGTACA 2 cut(s) 638, 1084
Bsp143I GATC 8 cut(s) 14, 111, 236, 382, 577, 800, 1274, 1543
Bsp19I CCATGG 1 cut(s) 495
BspACI CCGC 3 cut(s) 1064, 1066, 1208
BspANI GGCC 3 cut(s) 167, 429, 512
BspCNI CTCAG 3 cut(s) 562, 1501, 1594
BspFNI CGCG 1 cut(s) 1066
BspHI TCATGA 3 cut(s) 991, 1390, 1431
BspLI GGNNCC 2 cut(s) 428, 949
BspMAI CTGCAG 1 cut(s) 293
BspMI ACCTGC 1 cut(s) 886
BspPI GGATC 6 cut(s) 22, 106, 231, 390, 572, 1282
BspQI GCTCTTC 1 cut(s) 1299
BspTNI GGTCTC 1 cut(s) 386
BsrGI TGTACA 2 cut(s) 638, 1084
BsrI ACTGG 2 cut(s) 375, 810
BssECI CCNNGG 6 cut(s) 51, 199, 396, 397, 495, 1064
BssMI GATC 8 cut(s) 14, 111, 236, 382, 577, 800, 1274, 1543
BssT1I CCWWGG 1 cut(s) 495
Bst2UI CCWGG 3 cut(s) 52, 200, 398
Bst4CI ACNGT 8 cut(s) 226, 389, 556, 666, 1043, 1216, 1411, 1493
Bst6I CTCTTC 2 cut(s) 1248, 1299
BstAUI TGTACA 2 cut(s) 638, 1084
BstC8I GCNNGC 2 cut(s) 360, 1338
BstDEI CTNAG 4 cut(s) 549, 620, 1509, 1602
BstDSI CCRYGG 2 cut(s) 495, 1064
BstEII GGTNACC 1 cut(s) 1627
BstF5I GGATG 3 cut(s) 20, 817, 870
BstFNI CGCG 1 cut(s) 1066
BstKTI GATC 8 cut(s) 17, 114, 239, 385, 580, 803, 1277, 1546
BstMAI GTCTC 1 cut(s) 386
BstMBI GATC 8 cut(s) 14, 111, 236, 382, 577, 800, 1274, 1543
BstMWI GCNNNNNNNGC 5 cut(s) 62, 892, 977, 1306, 1589
BstNI CCWGG 3 cut(s) 52, 200, 398
BstPI GGTNACC 1 cut(s) 1627
BstSCI CCNGG 3 cut(s) 50, 198, 396
BstSFI CTRYAG 2 cut(s) 289, 825
BstUI CGCG 1 cut(s) 1066
BstV1I GCAGC 5 cut(s) 74, 374, 377, 1304, 1431
BstX2I RGATCY 1 cut(s) 236
BstYI RGATCY 1 cut(s) 236
BsuI GTATCC 1 cut(s) 1146
BsuRI GGCC 3 cut(s) 167, 429, 512
BtgI CCRYGG 2 cut(s) 495, 1064
BtrI CACGTC 1 cut(s) 883
BtsCI GGATG 3 cut(s) 20, 817, 870
BtsI GCAGTG 2 cut(s) 1302, 1337
BtsIMutI CAGTG 7 cut(s) 382, 385, 561, 708, 803, 1302, 1337
BveI ACCTGC 1 cut(s) 886
Cac8I GCNNGC 2 cut(s) 360, 1338
CaiI CAGNNNCTG 1 cut(s) 368
CciI TCATGA 3 cut(s) 991, 1390, 1431
Cfr13I GGNCC 3 cut(s) 427, 510, 1216
Cfr42I CCGCGG 1 cut(s) 1067
CseI GACGC 1 cut(s) 719
Csp6I GTAC 4 cut(s) 639, 1085, 1193, 1607
CspCI CAANNNNNGTGG 2 cut(s) 250, 285
CviQI GTAC 4 cut(s) 639, 1085, 1193, 1607
DdeI CTNAG 4 cut(s) 549, 620, 1509, 1602
DpnI GATC 8 cut(s) 16, 113, 238, 384, 579, 802, 1276, 1545
DpnII GATC 8 cut(s) 14, 111, 236, 382, 577, 800, 1274, 1543
Eam1104I CTCTTC 2 cut(s) 1248, 1299
EarI CTCTTC 2 cut(s) 1248, 1299
Ecl136II GAGCTC 1 cut(s) 1019
Eco130I CCWWGG 1 cut(s) 495
Eco24I GRGCYC 1 cut(s) 1021
Eco31I GGTCTC 1 cut(s) 386
Eco47I GGWCC 1 cut(s) 1216
Eco53kI GAGCTC 1 cut(s) 1019
Eco91I GGTNACC 1 cut(s) 1627
EcoICRI GAGCTC 1 cut(s) 1019
EcoO109I RGGNCCY 1 cut(s) 427
EcoO65I GGTNACC 1 cut(s) 1627
EcoRI GAATTC 1 cut(s) 1435
EcoRII CCWGG 3 cut(s) 50, 198, 396
EcoT14I CCWWGG 1 cut(s) 495
EcoT22I ATGCAT 2 cut(s) 257, 775
EcoT38I GRGCYC 1 cut(s) 1021
ErhI CCWWGG 1 cut(s) 495
FalI AAGNNNNNCTT 2 cut(s) 140, 172
FaqI GGGAC 1 cut(s) 1446
FauNDI CATATG 3 cut(s) 627, 1130, 1318
Fnu4HI GCNGC 5 cut(s) 63, 363, 366, 1293, 1420
FokI GGATG 3 cut(s) 7, 824, 857
FriOI GRGCYC 1 cut(s) 1021
Fsp4HI GCNGC 5 cut(s) 63, 363, 366, 1293, 1420
FspBI CTAG 5 cut(s) 938, 1259, 1500, 1580, 1584
GluI GCNGC 5 cut(s) 63, 363, 366, 1293, 1420
GsaI CCCAGC 1 cut(s) 1426
GsuI CTGGAG 2 cut(s) 30, 827
HaeIII GGCC 3 cut(s) 167, 429, 512
HapII CCGG 2 cut(s) 685, 1622
HgaI GACGC 1 cut(s) 719
HincII GTYRAC 1 cut(s) 715
HindII GTYRAC 1 cut(s) 715
HinfI GANTC 5 cut(s) 748, 834, 1009, 1078, 1400
HpaII CCGG 2 cut(s) 685, 1622
HphI GGTGA 4 cut(s) 484, 1222, 1487, 1621
Hpy166II GTNNAC 3 cut(s) 32, 597, 715
Hpy188I TCNGA 2 cut(s) 552, 833
Hpy188III TCNNGA 9 cut(s) 9, 38, 992, 1075, 1138, 1285, 1391, 1432, 1550
Hpy8I GTNNAC 3 cut(s) 32, 597, 715
Hpy99I CGWCG 1 cut(s) 884
HpyAV CCTTC 1 cut(s) 501
HpyCH4III ACNGT 8 cut(s) 226, 389, 556, 666, 1043, 1216, 1411, 1493
HpyCH4IV ACGT 1 cut(s) 882
HpyF10VI GCNNNNNNNGC 5 cut(s) 62, 892, 977, 1306, 1589
HpyF3I CTNAG 4 cut(s) 549, 620, 1509, 1602
HpySE526I ACGT 1 cut(s) 882
KspI CCGCGG 1 cut(s) 1067
Kzo9I GATC 8 cut(s) 14, 111, 236, 382, 577, 800, 1274, 1543
LguI GCTCTTC 1 cut(s) 1299
LmnI GCTCC 2 cut(s) 1016, 1024
Lsp1109I GCAGC 5 cut(s) 74, 374, 377, 1304, 1431
LweI GCATC 1 cut(s) 29
MaeI CTAG 5 cut(s) 938, 1259, 1500, 1580, 1584
MaeII ACGT 1 cut(s) 882
MaeIII GTNAC 9 cut(s) 210, 1043, 1135, 1210, 1232, 1261, 1493, 1523, 1627
MalI GATC 8 cut(s) 16, 113, 238, 384, 579, 802, 1276, 1545
MboI GATC 8 cut(s) 14, 111, 236, 382, 577, 800, 1274, 1543
MboII GAAGA 9 cut(s) 673, 859, 1117, 1235, 1316, 1409, 1443, 1541, 1553
MfeI CAATTG 1 cut(s) 1188
MflI RGATCY 1 cut(s) 236
MhlI GDGCHC 2 cut(s) 108, 1021
MluCI AATT 9 cut(s) 216, 927, 1145, 1188, 1279, 1435, 1513, 1533, 1572
MlyI GAGTC 1 cut(s) 742
MmeI TCCRAC 4 cut(s) 640, 782, 811, 1540
Mph1103I ATGCAT 2 cut(s) 257, 775
MseI TTAA 1 cut(s) 1645
MslI CAYNNNNRTG 1 cut(s) 1341
MspA1I CMGCKG 3 cut(s) 368, 1066, 1422
MspI CCGG 2 cut(s) 685, 1622
MspR9I CCNGG 3 cut(s) 52, 200, 398
MunI CAATTG 1 cut(s) 1188
Mva1269I GAATGC 4 cut(s) 360, 773, 1111, 1623
MvaI CCWGG 3 cut(s) 52, 200, 398
MvnI CGCG 1 cut(s) 1066
MwoI GCNNNNNNNGC 5 cut(s) 62, 892, 977, 1306, 1589
NcoI CCATGG 1 cut(s) 495
NdeI CATATG 3 cut(s) 627, 1130, 1318
NdeII GATC 8 cut(s) 14, 111, 236, 382, 577, 800, 1274, 1543
NlaIV GGNNCC 2 cut(s) 428, 949
NmuCI GTSAC 7 cut(s) 210, 1043, 1135, 1210, 1261, 1493, 1627
NsiI ATGCAT 2 cut(s) 257, 775
PagI TCATGA 3 cut(s) 991, 1390, 1431
PaqCI CACCTGC 1 cut(s) 886
PasI CCCWGGG 1 cut(s) 397
PciSI GCTCTTC 1 cut(s) 1299
PctI GAATGC 4 cut(s) 360, 773, 1111, 1623
PfeI GAWTC 4 cut(s) 834, 1009, 1078, 1400
PkrI GCNGC 5 cut(s) 64, 364, 367, 1294, 1421
PleI GAGTC 1 cut(s) 742
PpsI GAGTC 1 cut(s) 742
PsiI TTATAA 1 cut(s) 1325
Psp124BI GAGCTC 1 cut(s) 1021
Psp6I CCWGG 3 cut(s) 50, 198, 396
PspEI GGTNACC 1 cut(s) 1627
PspFI CCCAGC 1 cut(s) 1422
PspGI CCWGG 3 cut(s) 50, 198, 396
PspN4I GGNNCC 2 cut(s) 428, 949
PspPI GGNCC 3 cut(s) 427, 510, 1216
PstI CTGCAG 1 cut(s) 293
PstNI CAGNNNCTG 1 cut(s) 368
PsuI RGATCY 1 cut(s) 236
PvuII CAGCTG 2 cut(s) 368, 1422
RsaI GTAC 4 cut(s) 640, 1086, 1194, 1608
RsaNI GTAC 4 cut(s) 639, 1085, 1193, 1607
RseI CAYNNNNRTG 1 cut(s) 1341
SacI GAGCTC 1 cut(s) 1021
SacII CCGCGG 1 cut(s) 1067
SapI GCTCTTC 1 cut(s) 1299
SaqAI TTAA 1 cut(s) 1645
SatI GCNGC 5 cut(s) 63, 363, 366, 1293, 1420
Sau3AI GATC 8 cut(s) 14, 111, 236, 382, 577, 800, 1274, 1543
Sau96I GGNCC 3 cut(s) 427, 510, 1216
SchI GAGTC 1 cut(s) 742
ScrFI CCNGG 3 cut(s) 52, 200, 398
SduI GDGCHC 2 cut(s) 108, 1021
SfaNI GCATC 1 cut(s) 29
SfcI CTRYAG 2 cut(s) 289, 825
Sfr303I CCGCGG 1 cut(s) 1067
SgrBI CCGCGG 1 cut(s) 1067
SinI GGWCC 1 cut(s) 1216
SmiMI CAYNNNNRTG 1 cut(s) 1341
SmlI CTYRAG 1 cut(s) 420
SmoI CTYRAG 1 cut(s) 420
Sse9I AATT 9 cut(s) 216, 927, 1145, 1188, 1279, 1435, 1513, 1533, 1572
SsiI CCGC 3 cut(s) 1064, 1066, 1208
SspMI CTAG 5 cut(s) 938, 1259, 1500, 1580, 1584
SstI GAGCTC 1 cut(s) 1021
StyD4I CCNGG 3 cut(s) 50, 198, 396
StyI CCWWGG 1 cut(s) 495
TaaI ACNGT 8 cut(s) 226, 389, 556, 666, 1043, 1216, 1411, 1493
TaiI ACGT 1 cut(s) 885
TaqI TCGA 4 cut(s) 162, 746, 777, 879
TasI AATT 9 cut(s) 216, 927, 1145, 1188, 1279, 1435, 1513, 1533, 1572
TatI WGTACW 2 cut(s) 638, 1084
TfiI GAWTC 4 cut(s) 834, 1009, 1078, 1400
Tru1I TTAA 1 cut(s) 1645
Tru9I TTAA 1 cut(s) 1645
TscAI CASTG 7 cut(s) 382, 392, 561, 708, 810, 1302, 1337
TseFI GTSAC 7 cut(s) 210, 1043, 1135, 1210, 1261, 1493, 1627
TseI GCWGC 5 cut(s) 62, 362, 365, 1292, 1419
Tsp45I GTSAC 7 cut(s) 210, 1043, 1135, 1210, 1261, 1493, 1627
TspRI CASTG 7 cut(s) 382, 392, 561, 708, 810, 1302, 1337
VpaK11BI GGWCC 1 cut(s) 1216
XapI RAATTY 3 cut(s) 1279, 1435, 1513
XcmI CCANNNNNNNNNTGG 1 cut(s) 55
XspI CTAG 5 cut(s) 938, 1259, 1500, 1580, 1584
Zsp2I ATGCAT 2 cut(s) 257, 775
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.