AT3G45980

histone H2B

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0000578
Physical Location & Seq
Reverse (-)
16897287 .. 16898013
727 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G45980.1

Sequence Viewer

Length: 453 bp
ATGGCGCCGAGAGCAGAGAAGAAGCCCGCGGAGAAGAAACCAGCCGCCGAGAAACCAGTAGAGGAGAAATCAAAAGCCGAGAAAGCTCCGGCGGAGAAGAAACCAAAAGCCGGCAAGAAACTCCCGAAGGAAGCCGGGGCCGGCGGCGATAAGAAGAAGAAGATGAAGAAGAAGAGTGTGGAAACTTACAAGATCTACATCTTCAAGGTTCTGAAACAAGTTCATCCAGATATTGGTATTTCAAGCAAGGCTATGGGTATTATGAACAGTTTCATCAACGACATCTTCGAGAAATTGGCATCGGAATCTTCAAAGCTCGCTAGGTATAACAAGAAGCCGACGATTACTTCTCGGGAGATTCAGACTGCTGTTAGACTCGTTCTTCCTGGTGAGCTCGCTAAACACGCTGTTTCTGAAGGAACCAAGGCTGTTACCAAATTCACAAGCTCTTGA

Protein Analysis

150

Amino Acids

16.44

Weight (kDa)

10.02

Isoelectric Point (pI)

26.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Histone PF00125 50 - 126 1.1e-20 Core histone H2A/H2B/H3/H4 domain
Histone_ABTB PF26281 80 - 149 7.2e-07 Histone domain in ABTB family protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000578)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G45980 AT3G46030 AT5G59910
fragaria_vesca FvH4_3g35121 FvH4_3g35121 FvH4_3g35121 FvH4_3g35180 FvH4_3g35380 FvH4_3g35390 FvH4_3g35440 FvH4_3g40690
malus_domestica MD07G1116500.v1.1 MD11G1112800.v1.1 MD11G1144100.v1.1
prunus_persica Prupe.6G082000_v2.0.a1 Prupe.6G082700_v2.0.a1 Prupe.6G082700_v2.0.a1 Prupe.6G082700_v2.0.a1 Prupe.6G082700_v2.0.a1 Prupe.6G082700_v2.0.a1 Prupe.6G082700_v2.0.a1 Prupe.6G083500_v2.0.a1
pyrus_communis pycom03g08040 pycom11g09350
rosa_chinensis RchiOBHm_Chr5g0063141 RchiOBHm_Chr5g0063151 RchiOBHm_Chr5g0063491 RchiOBHm_Chr5g0063501 RchiOBHm_Chr5g0063561 RchiOBHm_Chr5g0073451
rosa_laevigata RLG00000035607 RLG00000035610 RLG00000035611 RLG00000035640 RLG00000035650 RLG00000035655
rosa_multiflora Rmu_sc0005045.1_g000027 Rmu_sc0005045.1_g000029 Rmu_sc0005045.1_g000036 Rmu_sc0016394.1_g000001 Rmu_sc0021593.1_g000004 Rmu_ssc0000309.1_g000057 Rmu_ssc0000309.1_g000058
rosa_roxburghii Rroxscaffold_1G00007800 Rroxscaffold_1G00017210 Rroxscaffold_1G00017490 Rroxscaffold_1G00017500
rosa_rugosa Rorug05G0354400 Rorug05G0354600 Rorug05G0354700 Rorug05G0357700 Rorug05G0357800 Rorug05G0358300 Rorug05G0423500
rosa_samantha Rh5AG415000 Rh5AG416900 Rh5AG480900 Rh5BG429700 Rh5BG430000 Rh5BG432000 Rh5BG432200 Rh5BG432500 Rh5BG501700 Rh5CG453200 Rh5CG453500 Rh5CG455300 Rh5CG455600 Rh5CG455700 Rh5CG524800 Rh5DG443200 Rh5DG443400 Rh5DG443500 Rh5DG445400 Rh5DG445600 Rh5DG445900 Rh5DG514000
rosa_wichuraiana Rw5G038990 Rw5G039010 Rw5G039160 Rw5G039180 Rw5G044680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 4
AccB7I CCANNNNNTGG 1 cut(s) 233
AccII CGCG 1 cut(s) 29
AciI CCGC 5 cut(s) 27, 29, 45, 92, 144
AcsI RAATTY 1 cut(s) 437
AcuI CTGAAG 1 cut(s) 435
AcyI GRCGYC 1 cut(s) 5
AfiI CCNNNNNNNGG 2 cut(s) 110, 233
AgsI TTSAA 3 cut(s) 205, 243, 312
AjnI CCWGG 1 cut(s) 385
AluBI AGCT 4 cut(s) 86, 316, 394, 447
AluI AGCT 4 cut(s) 86, 316, 394, 447
Alw21I GWGCWC 1 cut(s) 396
Ama87I CYCGRG 1 cut(s) 351
AoxI GGCC 1 cut(s) 138
ApoI RAATTY 1 cut(s) 437
Asp700I GAANNNNTTC 1 cut(s) 269
AspLEI GCGC 1 cut(s) 7
AspS9I GGNCC 1 cut(s) 138
AsuC2I CCSGG 1 cut(s) 136
AsuHPI GGTGA 1 cut(s) 401
AvaI CYCGRG 1 cut(s) 351
BanI GGYRCC 1 cut(s) 4
BanII GRGCYC 1 cut(s) 396
Bbv12I GWGCWC 1 cut(s) 396
BciT130I CCWGG 1 cut(s) 387
BcnI CCSGG 1 cut(s) 136
BfaI CTAG 1 cut(s) 321
BfoI RGCGCY 1 cut(s) 8
BglII AGATCT 1 cut(s) 192
BisI GCNGC 2 cut(s) 45, 145
BlsI GCNGC 2 cut(s) 46, 146
Bme1390I CCNGG 2 cut(s) 136, 387
BmeT110I CYCGRG 1 cut(s) 351
BmgT120I GGNCC 1 cut(s) 138
BmiI GGNNCC 3 cut(s) 6, 139, 421
BmrFI CCNGG 2 cut(s) 136, 387
BmsI GCATC 1 cut(s) 308
BpuMI CCSGG 1 cut(s) 136
BsaBI GATNNNNATC 1 cut(s) 197
BsaHI GRCGYC 1 cut(s) 5
BsaJI CCNNGG 3 cut(s) 27, 135, 423
Bsc4I CCNNNNNNNGG 2 cut(s) 110, 233
Bse118I RCCGGY 2 cut(s) 110, 140
Bse1I ACTGG 1 cut(s) 56
Bse8I GATNNNNATC 1 cut(s) 197
BseBI CCWGG 1 cut(s) 387
BseDI CCNNGG 3 cut(s) 27, 135, 423
BseGI GGATG 1 cut(s) 223
BseJI GATNNNNATC 1 cut(s) 197
BseLI CCNNNNNNNGG 2 cut(s) 110, 233
BseNI ACTGG 1 cut(s) 56
BseRI GAGGAG 1 cut(s) 77
Bsh1236I CGCG 1 cut(s) 29
BshFI GGCC 1 cut(s) 140
BshNI GGYRCC 1 cut(s) 4
BsiHKAI GWGCWC 1 cut(s) 396
BsiHKCI CYCGRG 1 cut(s) 351
BsiSI CCGG 4 cut(s) 89, 111, 135, 141
BslI CCNNNNNNNGG 2 cut(s) 110, 233
BsnI GGCC 1 cut(s) 140
BsoBI CYCGRG 1 cut(s) 351
Bsp1286I GDGCHC 1 cut(s) 396
Bsp143I GATC 1 cut(s) 192
BspACI CCGC 5 cut(s) 27, 29, 45, 92, 144
BspANI GGCC 1 cut(s) 140
BspFNI CGCG 1 cut(s) 29
BspLI GGNNCC 3 cut(s) 6, 139, 421
BspT107I GGYRCC 1 cut(s) 4
BsrFI RCCGGY 2 cut(s) 110, 140
BsrI ACTGG 1 cut(s) 56
BssAI RCCGGY 2 cut(s) 110, 140
BssECI CCNNGG 3 cut(s) 27, 135, 423
BssMI GATC 1 cut(s) 192
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 1 cut(s) 423
Bst2UI CCWGG 1 cut(s) 387
Bst4CI ACNGT 1 cut(s) 269
Bst6I CTCTTC 1 cut(s) 167
BstACI GRCGYC 1 cut(s) 5
BstC8I GCNNGC 5 cut(s) 27, 112, 142, 318, 396
BstDSI CCRYGG 1 cut(s) 27
BstF5I GGATG 1 cut(s) 223
BstFNI CGCG 1 cut(s) 29
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 1 cut(s) 7
BstKTI GATC 1 cut(s) 195
BstMBI GATC 1 cut(s) 192
BstMWI GCNNNNNNNGC 3 cut(s) 11, 83, 404
BstNI CCWGG 1 cut(s) 387
BstSCI CCNGG 2 cut(s) 134, 385
BstUI CGCG 1 cut(s) 29
BstX2I RGATCY 1 cut(s) 192
BstYI RGATCY 1 cut(s) 192
BsuRI GGCC 1 cut(s) 140
BtgI CCRYGG 1 cut(s) 27
BtsCI GGATG 1 cut(s) 223
Cac8I GCNNGC 5 cut(s) 27, 112, 142, 318, 396
CfoI GCGC 1 cut(s) 7
Cfr10I RCCGGY 2 cut(s) 110, 140
Cfr13I GGNCC 1 cut(s) 138
Cfr42I CCGCGG 1 cut(s) 30
DinI GGCGCC 1 cut(s) 6
DpnI GATC 1 cut(s) 194
DpnII GATC 1 cut(s) 192
Eam1104I CTCTTC 1 cut(s) 167
EarI CTCTTC 1 cut(s) 167
EciI GGCGGA 1 cut(s) 107
Ecl136II GAGCTC 1 cut(s) 394
Eco130I CCWWGG 1 cut(s) 423
Eco24I GRGCYC 1 cut(s) 396
Eco53kI GAGCTC 1 cut(s) 394
Eco57I CTGAAG 1 cut(s) 435
Eco88I CYCGRG 1 cut(s) 351
EcoICRI GAGCTC 1 cut(s) 394
EcoRII CCWGG 1 cut(s) 385
EcoT14I CCWWGG 1 cut(s) 423
EcoT38I GRGCYC 1 cut(s) 396
EgeI GGCGCC 1 cut(s) 6
EheI GGCGCC 1 cut(s) 6
ErhI CCWWGG 1 cut(s) 423
FaiI YATR 3 cut(s) 254, 263, 327
FauI CCCGC 1 cut(s) 34
Fnu4HI GCNGC 2 cut(s) 45, 145
FokI GGATG 1 cut(s) 210
FriOI GRGCYC 1 cut(s) 396
Fsp4HI GCNGC 2 cut(s) 45, 145
FspBI CTAG 1 cut(s) 321
GlaI GCGC 1 cut(s) 6
GluI GCNGC 2 cut(s) 45, 145
HaeII RGCGCY 1 cut(s) 8
HaeIII GGCC 1 cut(s) 140
HapII CCGG 4 cut(s) 89, 111, 135, 141
HhaI GCGC 1 cut(s) 7
Hin1I GRCGYC 1 cut(s) 5
Hin6I GCGC 1 cut(s) 5
HinP1I GCGC 1 cut(s) 5
HinfI GANTC 3 cut(s) 305, 358, 375
HpaII CCGG 4 cut(s) 89, 111, 135, 141
HphI GGTGA 1 cut(s) 401
Hpy188I TCNGA 4 cut(s) 213, 304, 363, 415
Hpy188III TCNNGA 5 cut(s) 124, 227, 289, 353, 450
Hpy99I CGWCG 1 cut(s) 343
HpyAV CCTTC 2 cut(s) 121, 410
HpyCH4III ACNGT 1 cut(s) 269
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 83, 404
Hsp92I GRCGYC 1 cut(s) 5
HspAI GCGC 1 cut(s) 5
KasI GGCGCC 1 cut(s) 4
KroI GCCGGC 2 cut(s) 110, 140
KroNI GCCGGC 2 cut(s) 112, 142
KspI CCGCGG 1 cut(s) 30
Kzo9I GATC 1 cut(s) 192
LmnI GCTCC 1 cut(s) 91
LpnPI CCDG 9 cut(s) 54, 69, 102, 124, 148, 154, 240, 372, 399
LweI GCATC 1 cut(s) 308
MaeI CTAG 1 cut(s) 321
MaeIII GTNAC 1 cut(s) 430
MalI GATC 1 cut(s) 194
MboI GATC 1 cut(s) 192
MflI RGATCY 1 cut(s) 192
MhlI GDGCHC 1 cut(s) 396
MluCI AATT 2 cut(s) 293, 437
Mly113I GGCGCC 1 cut(s) 5
MlyI GAGTC 1 cut(s) 369
MnlI CCTC 1 cut(s) 55
MroNI GCCGGC 2 cut(s) 110, 140
MroXI GAANNNNTTC 1 cut(s) 269
MspA1I CMGCKG 1 cut(s) 29
MspI CCGG 4 cut(s) 89, 111, 135, 141
MspR9I CCNGG 2 cut(s) 136, 387
MvaI CCWGG 1 cut(s) 387
MvnI CGCG 1 cut(s) 29
MwoI GCNNNNNNNGC 3 cut(s) 11, 83, 404
NaeI GCCGGC 2 cut(s) 112, 142
NarI GGCGCC 1 cut(s) 5
NciI CCSGG 1 cut(s) 136
NdeII GATC 1 cut(s) 192
NgoMIV GCCGGC 2 cut(s) 110, 140
NlaIV GGNNCC 3 cut(s) 6, 139, 421
NmeAIII GCCGAG 3 cut(s) 33, 73, 103
PcsI WCGNNNNNNNCGW 1 cut(s) 285
PdiI GCCGGC 2 cut(s) 112, 142
PdmI GAANNNNTTC 1 cut(s) 269
PfeI GAWTC 2 cut(s) 305, 358
PflMI CCANNNNNTGG 1 cut(s) 233
PkrI GCNGC 2 cut(s) 46, 146
PleI GAGTC 1 cut(s) 369
PluTI GGCGCC 1 cut(s) 8
PpsI GAGTC 1 cut(s) 369
Psp124BI GAGCTC 1 cut(s) 396
Psp6I CCWGG 1 cut(s) 385
PspGI CCWGG 1 cut(s) 385
PspN4I GGNNCC 3 cut(s) 6, 139, 421
PspPI GGNCC 1 cut(s) 138
PsuI RGATCY 1 cut(s) 192
SacI GAGCTC 1 cut(s) 396
SacII CCGCGG 1 cut(s) 30
SatI GCNGC 2 cut(s) 45, 145
Sau3AI GATC 1 cut(s) 192
Sau96I GGNCC 1 cut(s) 138
SchI GAGTC 1 cut(s) 369
ScrFI CCNGG 2 cut(s) 136, 387
SduI GDGCHC 1 cut(s) 396
SetI ASST 6 cut(s) 88, 210, 318, 326, 396, 449
SfaNI GCATC 1 cut(s) 308
SfoI GGCGCC 1 cut(s) 6
Sfr303I CCGCGG 1 cut(s) 30
SgrBI CCGCGG 1 cut(s) 30
Sse9I AATT 2 cut(s) 293, 437
SsiI CCGC 5 cut(s) 27, 29, 45, 92, 144
SspDI GGCGCC 1 cut(s) 4
SspMI CTAG 1 cut(s) 321
SstI GAGCTC 1 cut(s) 396
StyD4I CCNGG 2 cut(s) 134, 385
StyI CCWWGG 1 cut(s) 423
TaaI ACNGT 1 cut(s) 269
TaqI TCGA 1 cut(s) 288
TasI AATT 2 cut(s) 293, 437
TauI GCSGC 2 cut(s) 47, 147
TfiI GAWTC 2 cut(s) 305, 358
TspDTI ATGAA 4 cut(s) 179, 212, 262, 278
Van91I CCANNNNNTGG 1 cut(s) 233
XapI RAATTY 1 cut(s) 437
XmnI GAANNNNTTC 1 cut(s) 269
XspI CTAG 1 cut(s) 321
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.