Rh5AG480900

histone H2B

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0000578
Physical Location & Seq
Reverse (-)
82473411 .. 82474535
1125 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG480900.1

Sequence Viewer

Length: 441 bp
ATGGCGCCCAAAGCCGAGAAGAAGCCCGCGGAGAAGAAGCCGGCCGAGAAGACGACGGTGGCCGAGAAAGCCCCGGCGGAGAAGAAGCCCAAGGCCGGAAAGAAGCTCCCGAAGGAAGCCGGAGCCGCCGCCGGAGACAAGAAGAAGAAGAGGGCCAAGAAGAGCGTGGAGACCTACAAGATCTACATCTTCAAGGTCTTGAAGCAGGTCCACCCTGACATCGGAATCTCCAGCAAGGCCATGGGCATCATGAACAGCTTCATCAACGACATCTTCGAGAAGCTCGCTCAGGAATCGTCGAGGCTCGCCAGGTACAACAAGAAGCCGACGATTACTTCCCGGGAGATCCAGACGGCGGTGAGGCTTGTGCTTCCCGGTGAATTGGCTAAGCATGCTGTTTCTGAGGGGACTAAGGCGGTGACCAAGTTCACAAGCTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05034 ko05203 ko05322 map05034 map05203 map05322
Pfam Domains
Protein Families
Histone

Protein Analysis

146

Amino Acids

15.96

Weight (kDa)

10.11

Isoelectric Point (pI)

25.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Histone PF00125 46 - 122 3.1e-20 Core histone H2A/H2B/H3/H4 domain
Histone_ABTB PF26281 76 - 145 4.4e-08 Histone domain in ABTB family protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000578)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G45980 AT3G46030 AT5G59910
fragaria_vesca FvH4_3g35121 FvH4_3g35121 FvH4_3g35121 FvH4_3g35180 FvH4_3g35380 FvH4_3g35390 FvH4_3g35440 FvH4_3g40690
malus_domestica MD07G1116500.v1.1 MD11G1112800.v1.1 MD11G1144100.v1.1
prunus_persica Prupe.6G082000_v2.0.a1 Prupe.6G082700_v2.0.a1 Prupe.6G082700_v2.0.a1 Prupe.6G082700_v2.0.a1 Prupe.6G082700_v2.0.a1 Prupe.6G082700_v2.0.a1 Prupe.6G082700_v2.0.a1 Prupe.6G083500_v2.0.a1
pyrus_communis pycom03g08040 pycom11g09350
rosa_chinensis RchiOBHm_Chr5g0063141 RchiOBHm_Chr5g0063151 RchiOBHm_Chr5g0063491 RchiOBHm_Chr5g0063501 RchiOBHm_Chr5g0063561 RchiOBHm_Chr5g0073451
rosa_laevigata RLG00000035607 RLG00000035610 RLG00000035611 RLG00000035640 RLG00000035650 RLG00000035655
rosa_multiflora Rmu_sc0005045.1_g000027 Rmu_sc0005045.1_g000029 Rmu_sc0005045.1_g000036 Rmu_sc0016394.1_g000001 Rmu_sc0021593.1_g000004 Rmu_ssc0000309.1_g000057 Rmu_ssc0000309.1_g000058
rosa_roxburghii Rroxscaffold_1G00007800 Rroxscaffold_1G00017210 Rroxscaffold_1G00017490 Rroxscaffold_1G00017500
rosa_rugosa Rorug05G0354400 Rorug05G0354600 Rorug05G0354700 Rorug05G0357700 Rorug05G0357800 Rorug05G0358300 Rorug05G0423500
rosa_samantha Rh5AG415000 Rh5AG416900 Rh5AG480900 Rh5BG429700 Rh5BG430000 Rh5BG432000 Rh5BG432200 Rh5BG432500 Rh5BG501700 Rh5CG453200 Rh5CG453500 Rh5CG455300 Rh5CG455600 Rh5CG455700 Rh5CG524800 Rh5DG443200 Rh5DG443400 Rh5DG443500 Rh5DG445400 Rh5DG445600 Rh5DG445900 Rh5DG514000
rosa_wichuraiana Rw5G038990 Rw5G039010 Rw5G039160 Rw5G039180 Rw5G044680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 196
AccB1I GGYRCC 1 cut(s) 4
AccII CGCG 1 cut(s) 29
AciI CCGC 7 cut(s) 27, 29, 77, 126, 129, 356, 416
AclWI GGATC 1 cut(s) 340
AcoI YGGCCR 2 cut(s) 42, 60
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 1 cut(s) 314
AfiI CCNNNNNNNGG 3 cut(s) 95, 221, 355
AgsI TTSAA 2 cut(s) 193, 202
AjnI CCWGG 1 cut(s) 308
AluBI AGCT 4 cut(s) 106, 258, 283, 435
AluI AGCT 4 cut(s) 106, 258, 283, 435
Alw26I GTCTC 2 cut(s) 129, 164
AlwI GGATC 1 cut(s) 340
Ama87I CYCGRG 1 cut(s) 339
AoxI GGCC 5 cut(s) 42, 60, 93, 153, 237
Asp700I GAANNNNTTC 1 cut(s) 257
AspLEI GCGC 1 cut(s) 7
AspS9I GGNCC 2 cut(s) 153, 208
AsuC2I CCSGG 4 cut(s) 74, 340, 341, 375
AsuHPI GGTGA 3 cut(s) 370, 389, 430
AvaI CYCGRG 1 cut(s) 339
AvaII GGWCC 1 cut(s) 208
BanI GGYRCC 1 cut(s) 4
BbsI GAAGAC 1 cut(s) 56
BceAI ACGGC 1 cut(s) 369
BciT130I CCWGG 1 cut(s) 310
BcnI CCSGG 4 cut(s) 74, 340, 341, 375
BcoDI GTCTC 2 cut(s) 129, 164
BfoI RGCGCY 1 cut(s) 8
BfuAI ACCTGC 1 cut(s) 196
BglII AGATCT 1 cut(s) 180
BisI GCNGC 2 cut(s) 126, 129
BlpI GCTNAGC 1 cut(s) 387
BlsI GCNGC 2 cut(s) 127, 130
Bme1390I CCNGG 5 cut(s) 74, 310, 340, 341, 375
Bme18I GGWCC 1 cut(s) 208
BmeT110I CYCGRG 1 cut(s) 339
BmgT120I GGNCC 2 cut(s) 153, 208
BmiI GGNNCC 2 cut(s) 6, 124
BmrFI CCNGG 5 cut(s) 74, 310, 340, 341, 375
BmsI GCATC 1 cut(s) 255
BpiI GAAGAC 1 cut(s) 56
BpmI CTGGAG 1 cut(s) 214
Bpu10I CCTNAGC 1 cut(s) 288
Bpu1102I GCTNAGC 1 cut(s) 387
BpuMI CCSGG 4 cut(s) 74, 340, 341, 375
BsaBI GATNNNNATC 1 cut(s) 185
BsaHI GRCGYC 1 cut(s) 5
BsaI GGTCTC 1 cut(s) 164
BsaJI CCNNGG 5 cut(s) 27, 72, 90, 240, 339
Bsc4I CCNNNNNNNGG 3 cut(s) 95, 221, 355
Bse118I RCCGGY 1 cut(s) 40
Bse8I GATNNNNATC 1 cut(s) 185
BseBI CCWGG 1 cut(s) 310
BseDI CCNNGG 5 cut(s) 27, 72, 90, 240, 339
BseJI GATNNNNATC 1 cut(s) 185
BseLI CCNNNNNNNGG 3 cut(s) 95, 221, 355
BseMII CTCAG 2 cut(s) 302, 393
BseX3I CGGCCG 1 cut(s) 42
Bsh1236I CGCG 1 cut(s) 29
Bsh1285I CGRYCG 1 cut(s) 45
BshFI GGCC 5 cut(s) 44, 62, 95, 155, 239
BshNI GGYRCC 1 cut(s) 4
BsiEI CGRYCG 1 cut(s) 45
BsiHKCI CYCGRG 1 cut(s) 339
BsiSI CCGG 7 cut(s) 41, 74, 96, 120, 132, 340, 375
BslFI GGGAC 1 cut(s) 421
BslI CCNNNNNNNGG 3 cut(s) 95, 221, 355
BsmAI GTCTC 2 cut(s) 129, 164
BsmFI GGGAC 1 cut(s) 421
BsnI GGCC 5 cut(s) 44, 62, 95, 155, 239
Bso31I GGTCTC 1 cut(s) 164
BsoBI CYCGRG 1 cut(s) 339
Bsp143I GATC 2 cut(s) 180, 345
Bsp1720I GCTNAGC 1 cut(s) 387
Bsp19I CCATGG 1 cut(s) 240
BspACI CCGC 7 cut(s) 27, 29, 77, 126, 129, 356, 416
BspANI GGCC 5 cut(s) 44, 62, 95, 155, 239
BspCNI CTCAG 2 cut(s) 301, 394
BspFNI CGCG 1 cut(s) 29
BspHI TCATGA 1 cut(s) 249
BspLI GGNNCC 2 cut(s) 6, 124
BspMI ACCTGC 1 cut(s) 196
BspPI GGATC 1 cut(s) 340
BspQI GCTCTTC 1 cut(s) 155
BspT107I GGYRCC 1 cut(s) 4
BspTNI GGTCTC 1 cut(s) 164
BsrFI RCCGGY 1 cut(s) 40
BssAI RCCGGY 1 cut(s) 40
BssECI CCNNGG 5 cut(s) 27, 72, 90, 240, 339
BssMI GATC 2 cut(s) 180, 345
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 2 cut(s) 90, 240
Bst2UI CCWGG 1 cut(s) 310
Bst4CI ACNGT 1 cut(s) 58
Bst6I CTCTTC 2 cut(s) 143, 155
BstACI GRCGYC 1 cut(s) 5
BstC8I GCNNGC 5 cut(s) 27, 42, 285, 306, 393
BstDEI CTNAG 4 cut(s) 288, 387, 402, 411
BstDSI CCRYGG 2 cut(s) 27, 240
BstEII GGTNACC 1 cut(s) 418
BstFNI CGCG 1 cut(s) 29
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 1 cut(s) 7
BstKTI GATC 2 cut(s) 183, 348
BstMAI GTCTC 2 cut(s) 129, 164
BstMBI GATC 2 cut(s) 180, 345
BstMCI CGRYCG 1 cut(s) 45
BstMWI GCNNNNNNNGC 4 cut(s) 11, 68, 125, 392
BstNI CCWGG 1 cut(s) 310
BstNSI RCATGY 1 cut(s) 395
BstPI GGTNACC 1 cut(s) 418
BstSCI CCNGG 5 cut(s) 72, 308, 338, 339, 373
BstUI CGCG 1 cut(s) 29
BstV2I GAAGAC 1 cut(s) 56
BstX2I RGATCY 2 cut(s) 180, 345
BstYI RGATCY 2 cut(s) 180, 345
BstZI CGGCCG 1 cut(s) 42
BsuRI GGCC 5 cut(s) 44, 62, 95, 155, 239
BtgI CCRYGG 2 cut(s) 27, 240
BveI ACCTGC 1 cut(s) 196
Cac8I GCNNGC 5 cut(s) 27, 42, 285, 306, 393
CciI TCATGA 1 cut(s) 249
CfoI GCGC 1 cut(s) 7
Cfr10I RCCGGY 1 cut(s) 40
Cfr13I GGNCC 2 cut(s) 153, 208
Cfr42I CCGCGG 1 cut(s) 30
Cfr9I CCCGGG 1 cut(s) 339
Csp6I GTAC 1 cut(s) 313
CviAII CATG 3 cut(s) 241, 250, 392
CviQI GTAC 1 cut(s) 313
DdeI CTNAG 4 cut(s) 288, 387, 402, 411
DinI GGCGCC 1 cut(s) 6
DpnI GATC 2 cut(s) 182, 347
DpnII GATC 2 cut(s) 180, 345
EaeI YGGCCR 2 cut(s) 42, 60
EagI CGGCCG 1 cut(s) 42
Eam1104I CTCTTC 2 cut(s) 143, 155
EarI CTCTTC 2 cut(s) 143, 155
EciI GGCGGA 1 cut(s) 92
EclXI CGGCCG 1 cut(s) 42
Eco130I CCWWGG 2 cut(s) 90, 240
Eco31I GGTCTC 1 cut(s) 164
Eco47I GGWCC 1 cut(s) 208
Eco52I CGGCCG 1 cut(s) 42
Eco88I CYCGRG 1 cut(s) 339
Eco91I GGTNACC 1 cut(s) 418
EcoO65I GGTNACC 1 cut(s) 418
EcoRII CCWGG 1 cut(s) 308
EcoT14I CCWWGG 2 cut(s) 90, 240
EgeI GGCGCC 1 cut(s) 6
EheI GGCGCC 1 cut(s) 6
ErhI CCWWGG 2 cut(s) 90, 240
FaeI CATG 3 cut(s) 244, 253, 395
FaiI YATR 3 cut(s) 242, 251, 393
FaqI GGGAC 1 cut(s) 421
FatI CATG 3 cut(s) 240, 249, 391
FauI CCCGC 1 cut(s) 34
Fnu4HI GCNGC 2 cut(s) 126, 129
Fsp4HI GCNGC 2 cut(s) 126, 129
GlaI GCGC 1 cut(s) 6
GluI GCNGC 2 cut(s) 126, 129
GsuI CTGGAG 1 cut(s) 214
HaeII RGCGCY 1 cut(s) 8
HaeIII GGCC 5 cut(s) 44, 62, 95, 155, 239
HapII CCGG 7 cut(s) 41, 74, 96, 120, 132, 340, 375
HhaI GCGC 1 cut(s) 7
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 3 cut(s) 244, 253, 395
Hin6I GCGC 1 cut(s) 5
HinP1I GCGC 1 cut(s) 5
HinfI GANTC 2 cut(s) 225, 293
HpaII CCGG 7 cut(s) 41, 74, 96, 120, 132, 340, 375
HphI GGTGA 3 cut(s) 370, 389, 430
Hpy166II GTNNAC 2 cut(s) 211, 429
Hpy188I TCNGA 2 cut(s) 224, 403
Hpy188III TCNNGA 7 cut(s) 109, 199, 250, 277, 290, 349, 438
Hpy8I GTNNAC 2 cut(s) 211, 429
Hpy99I CGWCG 3 cut(s) 58, 301, 331
HpyAV CCTTC 1 cut(s) 106
HpyCH4III ACNGT 1 cut(s) 58
HpyF10VI GCNNNNNNNGC 4 cut(s) 11, 68, 125, 392
HpyF3I CTNAG 4 cut(s) 288, 387, 402, 411
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 3 cut(s) 244, 253, 395
HspAI GCGC 1 cut(s) 5
KasI GGCGCC 1 cut(s) 4
KroI GCCGGC 1 cut(s) 40
KroNI GCCGGC 1 cut(s) 42
KspI CCGCGG 1 cut(s) 30
Kzo9I GATC 2 cut(s) 180, 345
LguI GCTCTTC 1 cut(s) 155
LmnI GCTCC 2 cut(s) 111, 122
LweI GCATC 1 cut(s) 255
MaeIII GTNAC 1 cut(s) 418
MalI GATC 2 cut(s) 182, 347
MboI GATC 2 cut(s) 180, 345
MflI RGATCY 2 cut(s) 180, 345
MluCI AATT 1 cut(s) 380
Mly113I GGCGCC 1 cut(s) 5
MnlI CCTC 4 cut(s) 144, 294, 354, 397
MroNI GCCGGC 1 cut(s) 40
MroXI GAANNNNTTC 1 cut(s) 257
MspA1I CMGCKG 1 cut(s) 29
MspI CCGG 7 cut(s) 41, 74, 96, 120, 132, 340, 375
MspR9I CCNGG 5 cut(s) 74, 310, 340, 341, 375
MvaI CCWGG 1 cut(s) 310
MvnI CGCG 1 cut(s) 29
MwoI GCNNNNNNNGC 4 cut(s) 11, 68, 125, 392
NaeI GCCGGC 1 cut(s) 42
NarI GGCGCC 1 cut(s) 5
NciI CCSGG 4 cut(s) 74, 340, 341, 375
NcoI CCATGG 1 cut(s) 240
NdeII GATC 2 cut(s) 180, 345
NgoMIV GCCGGC 1 cut(s) 40
NlaIII CATG 3 cut(s) 244, 253, 395
NlaIV GGNNCC 2 cut(s) 6, 124
NmeAIII GCCGAG 3 cut(s) 40, 70, 88
NmuCI GTSAC 1 cut(s) 418
NspI RCATGY 1 cut(s) 395
PaeI GCATGC 1 cut(s) 395
PagI TCATGA 1 cut(s) 249
PciSI GCTCTTC 1 cut(s) 155
PcsI WCGNNNNNNNCGW 1 cut(s) 273
PdiI GCCGGC 1 cut(s) 42
PdmI GAANNNNTTC 1 cut(s) 257
PfeI GAWTC 2 cut(s) 225, 293
PkrI GCNGC 2 cut(s) 127, 130
PluTI GGCGCC 1 cut(s) 8
Psp6I CCWGG 1 cut(s) 308
PspEI GGTNACC 1 cut(s) 418
PspGI CCWGG 1 cut(s) 308
PspN4I GGNNCC 2 cut(s) 6, 124
PspPI GGNCC 2 cut(s) 153, 208
PsuI RGATCY 2 cut(s) 180, 345
RsaI GTAC 1 cut(s) 314
RsaNI GTAC 1 cut(s) 313
SacII CCGCGG 1 cut(s) 30
SapI GCTCTTC 1 cut(s) 155
SatI GCNGC 2 cut(s) 126, 129
Sau3AI GATC 2 cut(s) 180, 345
Sau96I GGNCC 2 cut(s) 153, 208
ScrFI CCNGG 5 cut(s) 74, 310, 340, 341, 375
SetI ASST 8 cut(s) 108, 176, 198, 210, 260, 285, 314, 437
SfaNI GCATC 1 cut(s) 255
SfoI GGCGCC 1 cut(s) 6
Sfr303I CCGCGG 1 cut(s) 30
SgrBI CCGCGG 1 cut(s) 30
SinI GGWCC 1 cut(s) 208
SmaI CCCGGG 1 cut(s) 341
SphI GCATGC 1 cut(s) 395
Sse9I AATT 1 cut(s) 380
SsiI CCGC 7 cut(s) 27, 29, 77, 126, 129, 356, 416
SspDI GGCGCC 1 cut(s) 4
StyD4I CCNGG 5 cut(s) 72, 308, 338, 339, 373
StyI CCWWGG 2 cut(s) 90, 240
TaaI ACNGT 1 cut(s) 58
TaqI TCGA 2 cut(s) 276, 299
TasI AATT 1 cut(s) 380
TauI GCSGC 2 cut(s) 128, 131
TfiI GAWTC 2 cut(s) 225, 293
TseFI GTSAC 1 cut(s) 418
Tsp45I GTSAC 1 cut(s) 418
TspDTI ATGAA 2 cut(s) 250, 266
TspMI CCCGGG 1 cut(s) 339
VpaK11BI GGWCC 1 cut(s) 208
XceI RCATGY 1 cut(s) 395
XcmI CCANNNNNNNNNTGG 2 cut(s) 163, 238
XmaI CCCGGG 1 cut(s) 339
XmnI GAANNNNTTC 1 cut(s) 257
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.