Prupe.6G083500_v2.0.a1

histone H2B.1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp06
OG0000578
Physical Location & Seq
Reverse (-)
5636266 .. 5637186
921 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.6G083500.1

Sequence Viewer

Length: 447 bp
ATGGCGCCGAAAGCTGAGAAGAAGCCCGCCGAGAAGAAGCCAGCGGAGGAGAAGAAGTCGACTGTGGCTGAGAAAGCCCCGGCCGAGAAGAAGCCCAAGGCCGGCAAGAAGCTCCCCAAGGAGGCCGGAGCCGCCGCCGGAGATAAGAAGAAGAAGAGATCGAAGAAGAGCGTAGAGACCTACAAGATCTATATCTTCAAGGTGCTGAAGCAGGTTCACCCTGATATTGGGATCTCCAGCAAGGCCATGGGCATCATGAACAGCTTCATCAACGACATCTTTGAGAAGCTCGCCCAGGAGTCTTCCAGGCTTGCAAGGTACAACAAGAAGCCGACCATTACTTCTCGGGAGATCCAGACTGCTGTGAGGCTTGTGCTTCCTGGTGAGCTCGCTAAGCACGCGGTGTCTGAAGGGACTAAGGCGGTGACCAAGTTTACAAGTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05034 ko05203 ko05322 map05034 map05203 map05322
Pfam Domains
Protein Families
Histone

Protein Analysis

149

Amino Acids

16.22

Weight (kDa)

10.08

Isoelectric Point (pI)

29.99

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000578)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G45980 AT3G46030 AT5G59910
fragaria_vesca FvH4_3g35121 FvH4_3g35121 FvH4_3g35121 FvH4_3g35180 FvH4_3g35380 FvH4_3g35390 FvH4_3g35440 FvH4_3g40690
malus_domestica MD07G1116500.v1.1 MD11G1112800.v1.1 MD11G1144100.v1.1
prunus_persica Prupe.6G082000_v2.0.a1 Prupe.6G082700_v2.0.a1 Prupe.6G082700_v2.0.a1 Prupe.6G082700_v2.0.a1 Prupe.6G082700_v2.0.a1 Prupe.6G082700_v2.0.a1 Prupe.6G082700_v2.0.a1 Prupe.6G083500_v2.0.a1
pyrus_communis pycom03g08040 pycom11g09350
rosa_chinensis RchiOBHm_Chr5g0063141 RchiOBHm_Chr5g0063151 RchiOBHm_Chr5g0063491 RchiOBHm_Chr5g0063501 RchiOBHm_Chr5g0063561 RchiOBHm_Chr5g0073451
rosa_laevigata RLG00000035607 RLG00000035610 RLG00000035611 RLG00000035640 RLG00000035650 RLG00000035655
rosa_multiflora Rmu_sc0005045.1_g000027 Rmu_sc0005045.1_g000029 Rmu_sc0005045.1_g000036 Rmu_sc0016394.1_g000001 Rmu_sc0021593.1_g000004 Rmu_ssc0000309.1_g000057 Rmu_ssc0000309.1_g000058
rosa_roxburghii Rroxscaffold_1G00007800 Rroxscaffold_1G00017210 Rroxscaffold_1G00017490 Rroxscaffold_1G00017500
rosa_rugosa Rorug05G0354400 Rorug05G0354600 Rorug05G0354700 Rorug05G0357700 Rorug05G0357800 Rorug05G0358300 Rorug05G0423500
rosa_samantha Rh5AG415000 Rh5AG416900 Rh5AG480900 Rh5BG429700 Rh5BG430000 Rh5BG432000 Rh5BG432200 Rh5BG432500 Rh5BG501700 Rh5CG453200 Rh5CG453500 Rh5CG455300 Rh5CG455600 Rh5CG455700 Rh5CG524800 Rh5DG443200 Rh5DG443400 Rh5DG443500 Rh5DG445400 Rh5DG445600 Rh5DG445900 Rh5DG514000
rosa_wichuraiana Rw5G038990 Rw5G039010 Rw5G039160 Rw5G039180 Rw5G044680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 202
AccB1I GGYRCC 1 cut(s) 4
AccI GTMKAC 1 cut(s) 59
AccII CGCG 1 cut(s) 401
AciI CCGC 6 cut(s) 27, 44, 132, 135, 401, 422
AclWI GGATC 2 cut(s) 239, 346
AcoI YGGCCR 1 cut(s) 81
AcuI CTGAAG 2 cut(s) 227, 429
AcyI GRCGYC 1 cut(s) 5
AdeI CACNNNGTG 1 cut(s) 403
AfaI GTAC 1 cut(s) 320
AfiI CCNNNNNNNGG 3 cut(s) 101, 121, 227
AgsI TTSAA 1 cut(s) 199
AjnI CCWGG 3 cut(s) 294, 305, 379
AluBI AGCT 5 cut(s) 14, 112, 264, 289, 388
AluI AGCT 5 cut(s) 14, 112, 264, 289, 388
Alw21I GWGCWC 1 cut(s) 390
Alw26I GTCTC 1 cut(s) 170
AlwI GGATC 2 cut(s) 239, 346
Ama87I CYCGRG 1 cut(s) 345
AoxI GGCC 4 cut(s) 81, 99, 123, 243
Asp700I GAANNNNTTC 1 cut(s) 263
AspLEI GCGC 1 cut(s) 7
AsuC2I CCSGG 1 cut(s) 80
AsuHPI GGTGA 3 cut(s) 209, 395, 436
AvaI CYCGRG 1 cut(s) 345
BanI GGYRCC 1 cut(s) 4
BanII GRGCYC 1 cut(s) 390
BbsI GAAGAC 1 cut(s) 294
Bbv12I GWGCWC 1 cut(s) 390
BciT130I CCWGG 3 cut(s) 296, 307, 381
BcnI CCSGG 1 cut(s) 80
BcoDI GTCTC 1 cut(s) 170
BfoI RGCGCY 1 cut(s) 8
BfuAI ACCTGC 1 cut(s) 202
BglII AGATCT 1 cut(s) 186
BisI GCNGC 2 cut(s) 132, 135
BlpI GCTNAGC 1 cut(s) 393
BlsI GCNGC 2 cut(s) 133, 136
Bme1390I CCNGG 4 cut(s) 80, 296, 307, 381
BmeT110I CYCGRG 1 cut(s) 345
BmiI GGNNCC 2 cut(s) 6, 130
BmrFI CCNGG 4 cut(s) 80, 296, 307, 381
BmsI GCATC 1 cut(s) 261
BpiI GAAGAC 1 cut(s) 294
BpmI CTGGAG 1 cut(s) 220
Bpu1102I GCTNAGC 1 cut(s) 393
BpuMI CCSGG 1 cut(s) 80
BsaBI GATNNNNATC 1 cut(s) 191
BsaHI GRCGYC 1 cut(s) 5
BsaI GGTCTC 1 cut(s) 170
BsaJI CCNNGG 5 cut(s) 78, 96, 117, 246, 294
BsaXI ACNNNNNCTCC 2 cut(s) 41, 71
Bsc4I CCNNNNNNNGG 3 cut(s) 101, 121, 227
Bse118I RCCGGY 1 cut(s) 101
Bse8I GATNNNNATC 1 cut(s) 191
BseBI CCWGG 3 cut(s) 296, 307, 381
BseDI CCNNGG 5 cut(s) 78, 96, 117, 246, 294
BseJI GATNNNNATC 1 cut(s) 191
BseLI CCNNNNNNNGG 3 cut(s) 101, 121, 227
BseMII CTCAG 2 cut(s) 6, 60
BseRI GAGGAG 1 cut(s) 62
BseX3I CGGCCG 1 cut(s) 81
Bsh1236I CGCG 1 cut(s) 401
Bsh1285I CGRYCG 1 cut(s) 84
BshFI GGCC 4 cut(s) 83, 101, 125, 245
BshNI GGYRCC 1 cut(s) 4
BsiEI CGRYCG 1 cut(s) 84
BsiHKAI GWGCWC 1 cut(s) 390
BsiHKCI CYCGRG 1 cut(s) 345
BsiSI CCGG 4 cut(s) 80, 102, 126, 138
BslFI GGGAC 1 cut(s) 427
BslI CCNNNNNNNGG 3 cut(s) 101, 121, 227
BsmAI GTCTC 1 cut(s) 170
BsmFI GGGAC 1 cut(s) 427
BsnI GGCC 4 cut(s) 83, 101, 125, 245
Bso31I GGTCTC 1 cut(s) 170
BsoBI CYCGRG 1 cut(s) 345
Bsp1286I GDGCHC 1 cut(s) 390
Bsp143I GATC 4 cut(s) 158, 186, 231, 351
Bsp1720I GCTNAGC 1 cut(s) 393
Bsp19I CCATGG 1 cut(s) 246
BspACI CCGC 6 cut(s) 27, 44, 132, 135, 401, 422
BspANI GGCC 4 cut(s) 83, 101, 125, 245
BspCNI CTCAG 2 cut(s) 7, 61
BspFNI CGCG 1 cut(s) 401
BspHI TCATGA 1 cut(s) 255
BspLI GGNNCC 2 cut(s) 6, 130
BspMI ACCTGC 1 cut(s) 202
BspPI GGATC 2 cut(s) 239, 346
BspQI GCTCTTC 1 cut(s) 161
BspT107I GGYRCC 1 cut(s) 4
BspTNI GGTCTC 1 cut(s) 170
BsrFI RCCGGY 1 cut(s) 101
BssAI RCCGGY 1 cut(s) 101
BssECI CCNNGG 5 cut(s) 78, 96, 117, 246, 294
BssMI GATC 4 cut(s) 158, 186, 231, 351
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 3 cut(s) 96, 117, 246
Bst2UI CCWGG 3 cut(s) 296, 307, 381
Bst4CI ACNGT 1 cut(s) 64
Bst6I CTCTTC 2 cut(s) 149, 161
BstACI GRCGYC 1 cut(s) 5
BstC8I GCNNGC 7 cut(s) 27, 42, 103, 291, 312, 390, 399
BstDEI CTNAG 4 cut(s) 15, 69, 393, 417
BstDSI CCRYGG 1 cut(s) 246
BstEII GGTNACC 1 cut(s) 424
BstFNI CGCG 1 cut(s) 401
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 1 cut(s) 7
BstKTI GATC 4 cut(s) 161, 189, 234, 354
BstMAI GTCTC 1 cut(s) 170
BstMBI GATC 4 cut(s) 158, 186, 231, 351
BstMCI CGRYCG 1 cut(s) 84
BstMWI GCNNNNNNNGC 5 cut(s) 11, 74, 131, 394, 398
BstNI CCWGG 3 cut(s) 296, 307, 381
BstPI GGTNACC 1 cut(s) 424
BstSCI CCNGG 4 cut(s) 78, 294, 305, 379
BstUI CGCG 1 cut(s) 401
BstV2I GAAGAC 1 cut(s) 294
BstX2I RGATCY 3 cut(s) 186, 231, 351
BstYI RGATCY 3 cut(s) 186, 231, 351
BstZI CGGCCG 1 cut(s) 81
BsuRI GGCC 4 cut(s) 83, 101, 125, 245
BtgI CCRYGG 1 cut(s) 246
BveI ACCTGC 1 cut(s) 202
Cac8I GCNNGC 7 cut(s) 27, 42, 103, 291, 312, 390, 399
CciI TCATGA 1 cut(s) 255
CfoI GCGC 1 cut(s) 7
Cfr10I RCCGGY 1 cut(s) 101
Csp6I GTAC 1 cut(s) 319
CviAII CATG 2 cut(s) 247, 256
CviQI GTAC 1 cut(s) 319
DdeI CTNAG 4 cut(s) 15, 69, 393, 417
DinI GGCGCC 1 cut(s) 6
DpnI GATC 4 cut(s) 160, 188, 233, 353
DpnII GATC 4 cut(s) 158, 186, 231, 351
DraIII CACNNNGTG 1 cut(s) 403
EaeI YGGCCR 1 cut(s) 81
EagI CGGCCG 1 cut(s) 81
Eam1104I CTCTTC 2 cut(s) 149, 161
EarI CTCTTC 2 cut(s) 149, 161
Ecl136II GAGCTC 1 cut(s) 388
EclXI CGGCCG 1 cut(s) 81
Eco130I CCWWGG 3 cut(s) 96, 117, 246
Eco24I GRGCYC 1 cut(s) 390
Eco31I GGTCTC 1 cut(s) 170
Eco52I CGGCCG 1 cut(s) 81
Eco53kI GAGCTC 1 cut(s) 388
Eco57I CTGAAG 2 cut(s) 227, 429
Eco88I CYCGRG 1 cut(s) 345
Eco91I GGTNACC 1 cut(s) 424
EcoICRI GAGCTC 1 cut(s) 388
EcoO65I GGTNACC 1 cut(s) 424
EcoRII CCWGG 3 cut(s) 294, 305, 379
EcoT14I CCWWGG 3 cut(s) 96, 117, 246
EcoT38I GRGCYC 1 cut(s) 390
EgeI GGCGCC 1 cut(s) 6
EheI GGCGCC 1 cut(s) 6
ErhI CCWWGG 3 cut(s) 96, 117, 246
FaeI CATG 2 cut(s) 250, 259
FaiI YATR 3 cut(s) 192, 248, 257
FaqI GGGAC 1 cut(s) 427
FatI CATG 2 cut(s) 246, 255
FauI CCCGC 1 cut(s) 34
FblI GTMKAC 1 cut(s) 59
Fnu4HI GCNGC 2 cut(s) 132, 135
FriOI GRGCYC 1 cut(s) 390
Fsp4HI GCNGC 2 cut(s) 132, 135
GlaI GCGC 1 cut(s) 6
GluI GCNGC 2 cut(s) 132, 135
GsuI CTGGAG 1 cut(s) 220
HaeII RGCGCY 1 cut(s) 8
HaeIII GGCC 4 cut(s) 83, 101, 125, 245
HapII CCGG 4 cut(s) 80, 102, 126, 138
HhaI GCGC 1 cut(s) 7
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 2 cut(s) 250, 259
Hin6I GCGC 1 cut(s) 5
HinP1I GCGC 1 cut(s) 5
HincII GTYRAC 1 cut(s) 60
HindII GTYRAC 1 cut(s) 60
HinfI GANTC 1 cut(s) 299
HpaII CCGG 4 cut(s) 80, 102, 126, 138
HphI GGTGA 3 cut(s) 209, 395, 436
Hpy166II GTNNAC 3 cut(s) 60, 217, 435
Hpy188I TCNGA 1 cut(s) 409
Hpy188III TCNNGA 4 cut(s) 256, 347, 355, 444
Hpy8I GTNNAC 3 cut(s) 60, 217, 435
HpyAV CCTTC 1 cut(s) 404
HpyCH4III ACNGT 1 cut(s) 64
HpyCH4V TGCA 1 cut(s) 314
HpyF10VI GCNNNNNNNGC 5 cut(s) 11, 74, 131, 394, 398
HpyF3I CTNAG 4 cut(s) 15, 69, 393, 417
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 2 cut(s) 250, 259
HspAI GCGC 1 cut(s) 5
KasI GGCGCC 1 cut(s) 4
KroI GCCGGC 1 cut(s) 101
KroNI GCCGGC 1 cut(s) 103
Kzo9I GATC 4 cut(s) 158, 186, 231, 351
LguI GCTCTTC 1 cut(s) 161
LmnI GCTCC 2 cut(s) 117, 128
LweI GCATC 1 cut(s) 261
MaeIII GTNAC 1 cut(s) 424
MalI GATC 4 cut(s) 160, 188, 233, 353
MboI GATC 4 cut(s) 158, 186, 231, 351
MflI RGATCY 3 cut(s) 186, 231, 351
MhlI GDGCHC 1 cut(s) 390
Mly113I GGCGCC 1 cut(s) 5
MlyI GAGTC 1 cut(s) 308
MnlI CCTC 3 cut(s) 40, 115, 360
MroNI GCCGGC 1 cut(s) 101
MroXI GAANNNNTTC 1 cut(s) 263
MspA1I CMGCKG 1 cut(s) 44
MspI CCGG 4 cut(s) 80, 102, 126, 138
MspR9I CCNGG 4 cut(s) 80, 296, 307, 381
MvaI CCWGG 3 cut(s) 296, 307, 381
MvnI CGCG 1 cut(s) 401
MwoI GCNNNNNNNGC 5 cut(s) 11, 74, 131, 394, 398
NaeI GCCGGC 1 cut(s) 103
NarI GGCGCC 1 cut(s) 5
NciI CCSGG 1 cut(s) 80
NcoI CCATGG 1 cut(s) 246
NdeII GATC 4 cut(s) 158, 186, 231, 351
NgoMIV GCCGGC 1 cut(s) 101
NlaIII CATG 2 cut(s) 250, 259
NlaIV GGNNCC 2 cut(s) 6, 130
NmeAIII GCCGAG 2 cut(s) 55, 109
NmuCI GTSAC 1 cut(s) 424
PagI TCATGA 1 cut(s) 255
PciSI GCTCTTC 1 cut(s) 161
PdiI GCCGGC 1 cut(s) 103
PdmI GAANNNNTTC 1 cut(s) 263
PkrI GCNGC 2 cut(s) 133, 136
PleI GAGTC 1 cut(s) 307
PluTI GGCGCC 1 cut(s) 8
PpsI GAGTC 1 cut(s) 307
Psp124BI GAGCTC 1 cut(s) 390
Psp6I CCWGG 3 cut(s) 294, 305, 379
PspEI GGTNACC 1 cut(s) 424
PspGI CCWGG 3 cut(s) 294, 305, 379
PspN4I GGNNCC 2 cut(s) 6, 130
PsuI RGATCY 3 cut(s) 186, 231, 351
RsaI GTAC 1 cut(s) 320
RsaNI GTAC 1 cut(s) 319
SacI GAGCTC 1 cut(s) 390
SalI GTCGAC 1 cut(s) 58
SapI GCTCTTC 1 cut(s) 161
SatI GCNGC 2 cut(s) 132, 135
Sau3AI GATC 4 cut(s) 158, 186, 231, 351
SchI GAGTC 1 cut(s) 308
ScrFI CCNGG 4 cut(s) 80, 296, 307, 381
SduI GDGCHC 1 cut(s) 390
SetI ASST 9 cut(s) 16, 114, 182, 204, 216, 266, 291, 320, 390
SfaNI GCATC 1 cut(s) 261
SfoI GGCGCC 1 cut(s) 6
SsiI CCGC 6 cut(s) 27, 44, 132, 135, 401, 422
SspDI GGCGCC 1 cut(s) 4
SstI GAGCTC 1 cut(s) 390
StyD4I CCNGG 4 cut(s) 78, 294, 305, 379
StyI CCWWGG 3 cut(s) 96, 117, 246
TaaI ACNGT 1 cut(s) 64
TaqI TCGA 2 cut(s) 59, 161
TauI GCSGC 2 cut(s) 134, 137
TseFI GTSAC 1 cut(s) 424
Tsp45I GTSAC 1 cut(s) 424
TspDTI ATGAA 2 cut(s) 256, 272
XcmI CCANNNNNNNNNTGG 1 cut(s) 244
XmiI GTMKAC 1 cut(s) 59
XmnI GAANNNNTTC 1 cut(s) 263
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.