MD11G1112800.v1.1

histone H2B.1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0000578
Physical Location & Seq
Reverse (-)
10204662 .. 10205108
447 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1112800.v1.1.491

Sequence Viewer

Length: 447 bp
ATGGCGCCCAAAGCTGAGAAGAAGCCCGCCGAGAAGAAGCCAGCAGAGGAGAAGAAGTCGGCTGTCGCTGAGAAGGCCCCCGCCGAGAAGAAGCCCAAGGCCGGGAAGAAGCTCCCGAAGGAGGCTGGAGCCGCCGCCGGCGACAAGAAGAAGAAGAGATCGAAGAAGAGCGTGGAGACCTACAAGATCTACATCTTCAAGGTCCTGAAGCAGGTCCACCCTGACATCGGAATCTCCAGCAAGGCCATGGGGATCATGAACAGCTTCATCAACGACATCTTCGAGAAGCTCGCCCAGGAGTCCTCCAGGCTCGCCAGGTACAACAAGAAGCCCACCATCACTTCTCGGGAGATCCAGACTGCTGTGAGATTGGTGCTTCCTGGTGAGCTCGCTAAGCATGCGGTGTCCGAGGGGACTAAGGCGGTGACCAAGTTTACCAGCTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05034 ko05203 ko05322 map05034 map05203 map05322
Pfam Domains
Protein Families
Histone

Protein Analysis

149

Amino Acids

16.19

Weight (kDa)

10.08

Isoelectric Point (pI)

29.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Histone PF00125 48 - 124 1.1e-20 Core histone H2A/H2B/H3/H4 domain
Histone_ABTB PF26281 78 - 147 4.6e-08 Histone domain in ABTB family protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000578)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G45980 AT3G46030 AT5G59910
fragaria_vesca FvH4_3g35121 FvH4_3g35121 FvH4_3g35121 FvH4_3g35180 FvH4_3g35380 FvH4_3g35390 FvH4_3g35440 FvH4_3g40690
malus_domestica MD07G1116500.v1.1 MD11G1112800.v1.1 MD11G1144100.v1.1
prunus_persica Prupe.6G082000_v2.0.a1 Prupe.6G082700_v2.0.a1 Prupe.6G082700_v2.0.a1 Prupe.6G082700_v2.0.a1 Prupe.6G082700_v2.0.a1 Prupe.6G082700_v2.0.a1 Prupe.6G082700_v2.0.a1 Prupe.6G083500_v2.0.a1
pyrus_communis pycom03g08040 pycom11g09350
rosa_chinensis RchiOBHm_Chr5g0063141 RchiOBHm_Chr5g0063151 RchiOBHm_Chr5g0063491 RchiOBHm_Chr5g0063501 RchiOBHm_Chr5g0063561 RchiOBHm_Chr5g0073451
rosa_laevigata RLG00000035607 RLG00000035610 RLG00000035611 RLG00000035640 RLG00000035650 RLG00000035655
rosa_multiflora Rmu_sc0005045.1_g000027 Rmu_sc0005045.1_g000029 Rmu_sc0005045.1_g000036 Rmu_sc0016394.1_g000001 Rmu_sc0021593.1_g000004 Rmu_ssc0000309.1_g000057 Rmu_ssc0000309.1_g000058
rosa_roxburghii Rroxscaffold_1G00007800 Rroxscaffold_1G00017210 Rroxscaffold_1G00017490 Rroxscaffold_1G00017500
rosa_rugosa Rorug05G0354400 Rorug05G0354600 Rorug05G0354700 Rorug05G0357700 Rorug05G0357800 Rorug05G0358300 Rorug05G0423500
rosa_samantha Rh5AG415000 Rh5AG416900 Rh5AG480900 Rh5BG429700 Rh5BG430000 Rh5BG432000 Rh5BG432200 Rh5BG432500 Rh5BG501700 Rh5CG453200 Rh5CG453500 Rh5CG455300 Rh5CG455600 Rh5CG455700 Rh5CG524800 Rh5DG443200 Rh5DG443400 Rh5DG443500 Rh5DG445400 Rh5DG445600 Rh5DG445900 Rh5DG514000
rosa_wichuraiana Rw5G038990 Rw5G039010 Rw5G039160 Rw5G039180 Rw5G044680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 202
AccB1I GGYRCC 1 cut(s) 4
AciI CCGC 6 cut(s) 27, 81, 132, 135, 401, 422
AclWI GGATC 2 cut(s) 260, 346
AcuI CTGAAG 1 cut(s) 227
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 1 cut(s) 320
AfiI CCNNNNNNNGG 5 cut(s) 101, 102, 121, 211, 227
AgsI TTSAA 1 cut(s) 199
AjnI CCWGG 4 cut(s) 294, 305, 314, 379
AluBI AGCT 6 cut(s) 14, 112, 264, 289, 388, 441
AluI AGCT 6 cut(s) 14, 112, 264, 289, 388, 441
Alw21I GWGCWC 1 cut(s) 390
Alw26I GTCTC 1 cut(s) 170
AlwI GGATC 2 cut(s) 260, 346
Ama87I CYCGRG 1 cut(s) 345
AoxI GGCC 3 cut(s) 75, 99, 243
Asp700I GAANNNNTTC 1 cut(s) 263
AspLEI GCGC 1 cut(s) 7
AspS9I GGNCC 3 cut(s) 76, 202, 214
AsuC2I CCSGG 1 cut(s) 103
AsuHPI GGTGA 2 cut(s) 395, 436
AvaI CYCGRG 1 cut(s) 345
AvaII GGWCC 2 cut(s) 202, 214
BanI GGYRCC 1 cut(s) 4
BanII GRGCYC 1 cut(s) 390
Bbv12I GWGCWC 1 cut(s) 390
BccI CCATC 1 cut(s) 344
BciT130I CCWGG 4 cut(s) 296, 307, 316, 381
BcnI CCSGG 1 cut(s) 103
BcoDI GTCTC 1 cut(s) 170
BfoI RGCGCY 1 cut(s) 8
BfuAI ACCTGC 1 cut(s) 202
BglII AGATCT 1 cut(s) 186
BisI GCNGC 2 cut(s) 132, 135
BlpI GCTNAGC 1 cut(s) 393
BlsI GCNGC 2 cut(s) 133, 136
Bme1390I CCNGG 5 cut(s) 103, 296, 307, 316, 381
Bme18I GGWCC 2 cut(s) 202, 214
BmeT110I CYCGRG 1 cut(s) 345
BmgT120I GGNCC 3 cut(s) 76, 202, 214
BmiI GGNNCC 3 cut(s) 6, 78, 130
BmrFI CCNGG 5 cut(s) 103, 296, 307, 316, 381
BpmI CTGGAG 3 cut(s) 147, 220, 289
Bpu1102I GCTNAGC 1 cut(s) 393
BpuMI CCSGG 1 cut(s) 103
BsaBI GATNNNNATC 1 cut(s) 191
BsaHI GRCGYC 1 cut(s) 5
BsaI GGTCTC 1 cut(s) 170
BsaJI CCNNGG 4 cut(s) 96, 246, 294, 408
BsaXI ACNNNNNCTCC 2 cut(s) 41, 71
Bsc4I CCNNNNNNNGG 5 cut(s) 101, 102, 121, 211, 227
Bse118I RCCGGY 1 cut(s) 137
Bse8I GATNNNNATC 1 cut(s) 191
BseBI CCWGG 4 cut(s) 296, 307, 316, 381
BseDI CCNNGG 4 cut(s) 96, 246, 294, 408
BseJI GATNNNNATC 1 cut(s) 191
BseLI CCNNNNNNNGG 5 cut(s) 101, 102, 121, 211, 227
BseMII CTCAG 2 cut(s) 6, 60
BseRI GAGGAG 1 cut(s) 62
BshFI GGCC 3 cut(s) 77, 101, 245
BshNI GGYRCC 1 cut(s) 4
BsiHKAI GWGCWC 1 cut(s) 390
BsiHKCI CYCGRG 1 cut(s) 345
BsiSI CCGG 2 cut(s) 102, 138
BslFI GGGAC 1 cut(s) 427
BslI CCNNNNNNNGG 5 cut(s) 101, 102, 121, 211, 227
BsmAI GTCTC 1 cut(s) 170
BsmFI GGGAC 1 cut(s) 427
BsnI GGCC 3 cut(s) 77, 101, 245
Bso31I GGTCTC 1 cut(s) 170
BsoBI CYCGRG 1 cut(s) 345
Bsp1286I GDGCHC 1 cut(s) 390
Bsp143I GATC 4 cut(s) 158, 186, 252, 351
Bsp1720I GCTNAGC 1 cut(s) 393
Bsp19I CCATGG 1 cut(s) 246
BspACI CCGC 6 cut(s) 27, 81, 132, 135, 401, 422
BspANI GGCC 3 cut(s) 77, 101, 245
BspCNI CTCAG 2 cut(s) 7, 61
BspHI TCATGA 1 cut(s) 255
BspLI GGNNCC 3 cut(s) 6, 78, 130
BspMI ACCTGC 1 cut(s) 202
BspPI GGATC 2 cut(s) 260, 346
BspQI GCTCTTC 1 cut(s) 161
BspT107I GGYRCC 1 cut(s) 4
BspTNI GGTCTC 1 cut(s) 170
BsrFI RCCGGY 1 cut(s) 137
BssAI RCCGGY 1 cut(s) 137
BssECI CCNNGG 4 cut(s) 96, 246, 294, 408
BssMI GATC 4 cut(s) 158, 186, 252, 351
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 2 cut(s) 96, 246
Bst2UI CCWGG 4 cut(s) 296, 307, 316, 381
Bst6I CTCTTC 2 cut(s) 149, 161
BstACI GRCGYC 1 cut(s) 5
BstC8I GCNNGC 7 cut(s) 27, 42, 139, 291, 312, 390, 399
BstDEI CTNAG 4 cut(s) 15, 69, 393, 417
BstDSI CCRYGG 1 cut(s) 246
BstEII GGTNACC 1 cut(s) 424
BstENI CCTNNNNNAGG 1 cut(s) 209
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 1 cut(s) 7
BstKTI GATC 4 cut(s) 161, 189, 255, 354
BstMAI GTCTC 1 cut(s) 170
BstMBI GATC 4 cut(s) 158, 186, 252, 351
BstMWI GCNNNNNNNGC 5 cut(s) 11, 74, 131, 394, 398
BstNI CCWGG 4 cut(s) 296, 307, 316, 381
BstNSI RCATGY 1 cut(s) 401
BstPI GGTNACC 1 cut(s) 424
BstSCI CCNGG 5 cut(s) 101, 294, 305, 314, 379
BstX2I RGATCY 2 cut(s) 186, 351
BstYI RGATCY 2 cut(s) 186, 351
BsuRI GGCC 3 cut(s) 77, 101, 245
BtgI CCRYGG 1 cut(s) 246
BveI ACCTGC 1 cut(s) 202
Cac8I GCNNGC 7 cut(s) 27, 42, 139, 291, 312, 390, 399
CciI TCATGA 1 cut(s) 255
CfoI GCGC 1 cut(s) 7
Cfr10I RCCGGY 1 cut(s) 137
Cfr13I GGNCC 3 cut(s) 76, 202, 214
Csp6I GTAC 1 cut(s) 319
CviAII CATG 3 cut(s) 247, 256, 398
CviQI GTAC 1 cut(s) 319
DdeI CTNAG 4 cut(s) 15, 69, 393, 417
DinI GGCGCC 1 cut(s) 6
DpnI GATC 4 cut(s) 160, 188, 254, 353
DpnII GATC 4 cut(s) 158, 186, 252, 351
Eam1104I CTCTTC 2 cut(s) 149, 161
EarI CTCTTC 2 cut(s) 149, 161
Ecl136II GAGCTC 1 cut(s) 388
Eco130I CCWWGG 2 cut(s) 96, 246
Eco24I GRGCYC 1 cut(s) 390
Eco31I GGTCTC 1 cut(s) 170
Eco47I GGWCC 2 cut(s) 202, 214
Eco53kI GAGCTC 1 cut(s) 388
Eco57I CTGAAG 1 cut(s) 227
Eco88I CYCGRG 1 cut(s) 345
Eco91I GGTNACC 1 cut(s) 424
EcoICRI GAGCTC 1 cut(s) 388
EcoNI CCTNNNNNAGG 1 cut(s) 209
EcoO109I RGGNCCY 2 cut(s) 76, 202
EcoO65I GGTNACC 1 cut(s) 424
EcoRII CCWGG 4 cut(s) 294, 305, 314, 379
EcoT14I CCWWGG 2 cut(s) 96, 246
EcoT38I GRGCYC 1 cut(s) 390
EgeI GGCGCC 1 cut(s) 6
EheI GGCGCC 1 cut(s) 6
ErhI CCWWGG 2 cut(s) 96, 246
FaeI CATG 3 cut(s) 250, 259, 401
FaiI YATR 3 cut(s) 248, 257, 399
FaqI GGGAC 1 cut(s) 427
FatI CATG 3 cut(s) 246, 255, 397
FauI CCCGC 2 cut(s) 34, 88
Fnu4HI GCNGC 2 cut(s) 132, 135
FriOI GRGCYC 1 cut(s) 390
Fsp4HI GCNGC 2 cut(s) 132, 135
GlaI GCGC 1 cut(s) 6
GluI GCNGC 2 cut(s) 132, 135
GsuI CTGGAG 3 cut(s) 147, 220, 289
HaeII RGCGCY 1 cut(s) 8
HaeIII GGCC 3 cut(s) 77, 101, 245
HapII CCGG 2 cut(s) 102, 138
HhaI GCGC 1 cut(s) 7
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 3 cut(s) 250, 259, 401
Hin6I GCGC 1 cut(s) 5
HinP1I GCGC 1 cut(s) 5
HinfI GANTC 2 cut(s) 231, 299
HpaII CCGG 2 cut(s) 102, 138
HphI GGTGA 2 cut(s) 395, 436
Hpy166II GTNNAC 2 cut(s) 217, 435
Hpy188I TCNGA 2 cut(s) 230, 409
Hpy188III TCNNGA 7 cut(s) 115, 205, 256, 283, 347, 355, 444
Hpy8I GTNNAC 2 cut(s) 217, 435
HpyAV CCTTC 2 cut(s) 67, 112
HpyF10VI GCNNNNNNNGC 5 cut(s) 11, 74, 131, 394, 398
HpyF3I CTNAG 4 cut(s) 15, 69, 393, 417
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 3 cut(s) 250, 259, 401
HspAI GCGC 1 cut(s) 5
KasI GGCGCC 1 cut(s) 4
KroI GCCGGC 1 cut(s) 137
KroNI GCCGGC 1 cut(s) 139
Kzo9I GATC 4 cut(s) 158, 186, 252, 351
LguI GCTCTTC 1 cut(s) 161
LmnI GCTCC 2 cut(s) 117, 128
MaeIII GTNAC 1 cut(s) 424
MalI GATC 4 cut(s) 160, 188, 254, 353
MboI GATC 4 cut(s) 158, 186, 252, 351
MflI RGATCY 2 cut(s) 186, 351
MhlI GDGCHC 1 cut(s) 390
Mly113I GGCGCC 1 cut(s) 5
MlyI GAGTC 1 cut(s) 308
MnlI CCTC 4 cut(s) 40, 115, 313, 403
MreI CGCCGGCG 1 cut(s) 137
MroNI GCCGGC 1 cut(s) 137
MroXI GAANNNNTTC 1 cut(s) 263
MspI CCGG 2 cut(s) 102, 138
MspR9I CCNGG 5 cut(s) 103, 296, 307, 316, 381
MvaI CCWGG 4 cut(s) 296, 307, 316, 381
MwoI GCNNNNNNNGC 5 cut(s) 11, 74, 131, 394, 398
NaeI GCCGGC 1 cut(s) 139
NarI GGCGCC 1 cut(s) 5
NciI CCSGG 1 cut(s) 103
NcoI CCATGG 1 cut(s) 246
NdeII GATC 4 cut(s) 158, 186, 252, 351
NgoMIV GCCGGC 1 cut(s) 137
NlaIII CATG 3 cut(s) 250, 259, 401
NlaIV GGNNCC 3 cut(s) 6, 78, 130
NmeAIII GCCGAG 2 cut(s) 55, 109
NmuCI GTSAC 1 cut(s) 424
NspI RCATGY 1 cut(s) 401
PaeI GCATGC 1 cut(s) 401
PagI TCATGA 1 cut(s) 255
PciSI GCTCTTC 1 cut(s) 161
PcsI WCGNNNNNNNCGW 1 cut(s) 279
PdiI GCCGGC 1 cut(s) 139
PdmI GAANNNNTTC 1 cut(s) 263
PfeI GAWTC 1 cut(s) 231
PkrI GCNGC 2 cut(s) 133, 136
PleI GAGTC 1 cut(s) 307
PluTI GGCGCC 1 cut(s) 8
PpsI GAGTC 1 cut(s) 307
PpuMI RGGWCCY 1 cut(s) 202
Psp124BI GAGCTC 1 cut(s) 390
Psp5II RGGWCCY 1 cut(s) 202
Psp6I CCWGG 4 cut(s) 294, 305, 314, 379
PspEI GGTNACC 1 cut(s) 424
PspGI CCWGG 4 cut(s) 294, 305, 314, 379
PspN4I GGNNCC 3 cut(s) 6, 78, 130
PspPI GGNCC 3 cut(s) 76, 202, 214
PspPPI RGGWCCY 1 cut(s) 202
PsuI RGATCY 2 cut(s) 186, 351
RsaI GTAC 1 cut(s) 320
RsaNI GTAC 1 cut(s) 319
SacI GAGCTC 1 cut(s) 390
SapI GCTCTTC 1 cut(s) 161
SatI GCNGC 2 cut(s) 132, 135
Sau3AI GATC 4 cut(s) 158, 186, 252, 351
Sau96I GGNCC 3 cut(s) 76, 202, 214
SchI GAGTC 1 cut(s) 308
ScrFI CCNGG 5 cut(s) 103, 296, 307, 316, 381
SduI GDGCHC 1 cut(s) 390
SfoI GGCGCC 1 cut(s) 6
SgrAI CRCCGGYG 1 cut(s) 137
SinI GGWCC 2 cut(s) 202, 214
SphI GCATGC 1 cut(s) 401
SsiI CCGC 6 cut(s) 27, 81, 132, 135, 401, 422
SspDI GGCGCC 1 cut(s) 4
SstI GAGCTC 1 cut(s) 390
StyD4I CCNGG 5 cut(s) 101, 294, 305, 314, 379
StyI CCWWGG 2 cut(s) 96, 246
TaqI TCGA 2 cut(s) 161, 282
TauI GCSGC 2 cut(s) 134, 137
TfiI GAWTC 1 cut(s) 231
TseFI GTSAC 1 cut(s) 424
Tsp45I GTSAC 1 cut(s) 424
TspDTI ATGAA 2 cut(s) 256, 272
VpaK11BI GGWCC 2 cut(s) 202, 214
XagI CCTNNNNNAGG 1 cut(s) 209
XceI RCATGY 1 cut(s) 401
XcmI CCANNNNNNNNNTGG 1 cut(s) 244
XmnI GAANNNNTTC 1 cut(s) 263
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.