pycom11g09350

histone H2B

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0000578
Physical Location & Seq
Reverse (-)
7528558 .. 7528983
426 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom11g09350.1

Sequence Viewer

Length: 426 bp
ATGGCGCCCAAAGCTGAGAAGAAGCCCGCAGAGAAGTCCGCCGTGGCAGAGAAAGCCCCCGCCGAGAAGAAGCCCAAGGCCGGGAAGAAGCTCCCAAAGGAGGCCGGAGCCGCCGCCGGAGACAAGAAGAAGAAGAGATCGAAGAAGAGCGTGGAGACCTACAAGATCTACATATTCAAGGTCCTGAAGCAGGTCCACCCAGACATCGGAATCTCCAGCAAGGCCATGGGAATCATGAACAGCTTCATCAACGACATCTTCGAGAAGCTCGCCCAGGAGTCGTCCAGGCTCGCCAGGTACAACAAAAAACCGACTATCACTTCGAGGGAGATCCAGACGGCGGTGAGATTGGTGCTGCCCGGTGAGCTCGCCAAGCATGCGGTGTCTGAGGGTACTAAGGCGGTGACTAAGTTTACCAGCTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05034 ko05203 ko05322 map05034 map05203 map05322
Pfam Domains
Protein Families
Histone

Protein Analysis

142

Amino Acids

15.38

Weight (kDa)

10.11

Isoelectric Point (pI)

27.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Histone PF00125 41 - 117 9.5e-21 Core histone H2A/H2B/H3/H4 domain
Histone_ABTB PF26281 71 - 140 4e-08 Histone domain in ABTB family protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000578)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G45980 AT3G46030 AT5G59910
fragaria_vesca FvH4_3g35121 FvH4_3g35121 FvH4_3g35121 FvH4_3g35180 FvH4_3g35380 FvH4_3g35390 FvH4_3g35440 FvH4_3g40690
malus_domestica MD07G1116500.v1.1 MD11G1112800.v1.1 MD11G1144100.v1.1
prunus_persica Prupe.6G082000_v2.0.a1 Prupe.6G082700_v2.0.a1 Prupe.6G082700_v2.0.a1 Prupe.6G082700_v2.0.a1 Prupe.6G082700_v2.0.a1 Prupe.6G082700_v2.0.a1 Prupe.6G082700_v2.0.a1 Prupe.6G083500_v2.0.a1
pyrus_communis pycom03g08040 pycom11g09350
rosa_chinensis RchiOBHm_Chr5g0063141 RchiOBHm_Chr5g0063151 RchiOBHm_Chr5g0063491 RchiOBHm_Chr5g0063501 RchiOBHm_Chr5g0063561 RchiOBHm_Chr5g0073451
rosa_laevigata RLG00000035607 RLG00000035610 RLG00000035611 RLG00000035640 RLG00000035650 RLG00000035655
rosa_multiflora Rmu_sc0005045.1_g000027 Rmu_sc0005045.1_g000029 Rmu_sc0005045.1_g000036 Rmu_sc0016394.1_g000001 Rmu_sc0021593.1_g000004 Rmu_ssc0000309.1_g000057 Rmu_ssc0000309.1_g000058
rosa_roxburghii Rroxscaffold_1G00007800 Rroxscaffold_1G00017210 Rroxscaffold_1G00017490 Rroxscaffold_1G00017500
rosa_rugosa Rorug05G0354400 Rorug05G0354600 Rorug05G0354700 Rorug05G0357700 Rorug05G0357800 Rorug05G0358300 Rorug05G0423500
rosa_samantha Rh5AG415000 Rh5AG416900 Rh5AG480900 Rh5BG429700 Rh5BG430000 Rh5BG432000 Rh5BG432200 Rh5BG432500 Rh5BG501700 Rh5CG453200 Rh5CG453500 Rh5CG455300 Rh5CG455600 Rh5CG455700 Rh5CG524800 Rh5DG443200 Rh5DG443400 Rh5DG443500 Rh5DG445400 Rh5DG445600 Rh5DG445900 Rh5DG514000
rosa_wichuraiana Rw5G038990 Rw5G039010 Rw5G039160 Rw5G039180 Rw5G044680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 181
AccB1I GGYRCC 1 cut(s) 4
AciI CCGC 8 cut(s) 27, 39, 60, 111, 114, 341, 380, 401
AclWI GGATC 1 cut(s) 325
AcuI CTGAAG 1 cut(s) 206
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 2 cut(s) 299, 394
AfiI CCNNNNNNNGG 6 cut(s) 80, 81, 100, 190, 206, 340
AgsI TTSAA 1 cut(s) 178
AjnI CCWGG 3 cut(s) 273, 284, 293
AluBI AGCT 6 cut(s) 14, 91, 243, 268, 367, 420
AluI AGCT 6 cut(s) 14, 91, 243, 268, 367, 420
Alw21I GWGCWC 1 cut(s) 369
Alw26I GTCTC 2 cut(s) 114, 149
AlwI GGATC 1 cut(s) 325
AoxI GGCC 3 cut(s) 78, 102, 222
ApeKI GCWGC 1 cut(s) 355
ArsI GACNNNNNNTTYG 2 cut(s) 304, 336
Asp700I GAANNNNTTC 1 cut(s) 242
AspLEI GCGC 1 cut(s) 7
AspS9I GGNCC 2 cut(s) 181, 193
AsuC2I CCSGG 2 cut(s) 82, 360
AsuHPI GGTGA 3 cut(s) 355, 374, 415
AvaII GGWCC 2 cut(s) 181, 193
BanI GGYRCC 1 cut(s) 4
BanII GRGCYC 1 cut(s) 369
Bbv12I GWGCWC 1 cut(s) 369
BbvI GCAGC 1 cut(s) 342
BceAI ACGGC 2 cut(s) 26, 354
BciT130I CCWGG 3 cut(s) 275, 286, 295
BcnI CCSGG 2 cut(s) 82, 360
BcoDI GTCTC 2 cut(s) 114, 149
BfoI RGCGCY 1 cut(s) 8
BfuAI ACCTGC 1 cut(s) 181
BglII AGATCT 1 cut(s) 165
BisI GCNGC 3 cut(s) 111, 114, 356
BlsI GCNGC 3 cut(s) 112, 115, 357
Bme1390I CCNGG 5 cut(s) 82, 275, 286, 295, 360
Bme18I GGWCC 2 cut(s) 181, 193
BmgT120I GGNCC 2 cut(s) 181, 193
BmiI GGNNCC 2 cut(s) 6, 109
BmrFI CCNGG 5 cut(s) 82, 275, 286, 295, 360
BpmI CTGGAG 1 cut(s) 199
BpuMI CCSGG 2 cut(s) 82, 360
BsaHI GRCGYC 1 cut(s) 5
BsaI GGTCTC 1 cut(s) 149
BsaJI CCNNGG 4 cut(s) 42, 75, 225, 273
Bsc4I CCNNNNNNNGG 6 cut(s) 80, 81, 100, 190, 206, 340
BseBI CCWGG 3 cut(s) 275, 286, 295
BseDI CCNNGG 4 cut(s) 42, 75, 225, 273
BseLI CCNNNNNNNGG 6 cut(s) 80, 81, 100, 190, 206, 340
BseMII CTCAG 2 cut(s) 6, 378
BseXI GCAGC 1 cut(s) 342
BshFI GGCC 3 cut(s) 80, 104, 224
BshNI GGYRCC 1 cut(s) 4
BsiHKAI GWGCWC 1 cut(s) 369
BsiSI CCGG 4 cut(s) 81, 105, 117, 360
BslI CCNNNNNNNGG 6 cut(s) 80, 81, 100, 190, 206, 340
BsmAI GTCTC 2 cut(s) 114, 149
BsnI GGCC 3 cut(s) 80, 104, 224
Bso31I GGTCTC 1 cut(s) 149
Bsp1286I GDGCHC 1 cut(s) 369
Bsp143I GATC 3 cut(s) 137, 165, 330
Bsp19I CCATGG 1 cut(s) 225
BspACI CCGC 8 cut(s) 27, 39, 60, 111, 114, 341, 380, 401
BspANI GGCC 3 cut(s) 80, 104, 224
BspCNI CTCAG 2 cut(s) 7, 379
BspHI TCATGA 1 cut(s) 234
BspLI GGNNCC 2 cut(s) 6, 109
BspMI ACCTGC 1 cut(s) 181
BspPI GGATC 1 cut(s) 325
BspQI GCTCTTC 1 cut(s) 140
BspT107I GGYRCC 1 cut(s) 4
BspTNI GGTCTC 1 cut(s) 149
BssECI CCNNGG 4 cut(s) 42, 75, 225, 273
BssMI GATC 3 cut(s) 137, 165, 330
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 2 cut(s) 75, 225
Bst2UI CCWGG 3 cut(s) 275, 286, 295
Bst6I CTCTTC 2 cut(s) 128, 140
BstACI GRCGYC 1 cut(s) 5
BstC8I GCNNGC 5 cut(s) 27, 270, 291, 369, 378
BstDEI CTNAG 4 cut(s) 15, 387, 396, 408
BstDSI CCRYGG 2 cut(s) 42, 225
BstENI CCTNNNNNAGG 1 cut(s) 188
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 1 cut(s) 7
BstKTI GATC 3 cut(s) 140, 168, 333
BstMAI GTCTC 2 cut(s) 114, 149
BstMBI GATC 3 cut(s) 137, 165, 330
BstMWI GCNNNNNNNGC 6 cut(s) 11, 53, 110, 364, 373, 377
BstNI CCWGG 3 cut(s) 275, 286, 295
BstNSI RCATGY 1 cut(s) 380
BstSCI CCNGG 5 cut(s) 80, 273, 284, 293, 358
BstV1I GCAGC 1 cut(s) 342
BstX2I RGATCY 2 cut(s) 165, 330
BstYI RGATCY 2 cut(s) 165, 330
BsuRI GGCC 3 cut(s) 80, 104, 224
BtgI CCRYGG 2 cut(s) 42, 225
BveI ACCTGC 1 cut(s) 181
Cac8I GCNNGC 5 cut(s) 27, 270, 291, 369, 378
CciI TCATGA 1 cut(s) 234
CfoI GCGC 1 cut(s) 7
Cfr13I GGNCC 2 cut(s) 181, 193
Csp6I GTAC 2 cut(s) 298, 393
CviAII CATG 3 cut(s) 226, 235, 377
CviQI GTAC 2 cut(s) 298, 393
DdeI CTNAG 4 cut(s) 15, 387, 396, 408
DinI GGCGCC 1 cut(s) 6
DpnI GATC 3 cut(s) 139, 167, 332
DpnII GATC 3 cut(s) 137, 165, 330
Eam1104I CTCTTC 2 cut(s) 128, 140
EarI CTCTTC 2 cut(s) 128, 140
EciI GGCGGA 1 cut(s) 28
Ecl136II GAGCTC 1 cut(s) 367
Eco130I CCWWGG 2 cut(s) 75, 225
Eco24I GRGCYC 1 cut(s) 369
Eco31I GGTCTC 1 cut(s) 149
Eco47I GGWCC 2 cut(s) 181, 193
Eco53kI GAGCTC 1 cut(s) 367
Eco57I CTGAAG 1 cut(s) 206
EcoICRI GAGCTC 1 cut(s) 367
EcoNI CCTNNNNNAGG 1 cut(s) 188
EcoO109I RGGNCCY 1 cut(s) 181
EcoRII CCWGG 3 cut(s) 273, 284, 293
EcoT14I CCWWGG 2 cut(s) 75, 225
EcoT38I GRGCYC 1 cut(s) 369
EgeI GGCGCC 1 cut(s) 6
EheI GGCGCC 1 cut(s) 6
ErhI CCWWGG 2 cut(s) 75, 225
FaeI CATG 3 cut(s) 229, 238, 380
FaiI YATR 4 cut(s) 173, 227, 236, 378
FatI CATG 3 cut(s) 225, 234, 376
FauI CCCGC 2 cut(s) 34, 67
Fnu4HI GCNGC 3 cut(s) 111, 114, 356
FriOI GRGCYC 1 cut(s) 369
Fsp4HI GCNGC 3 cut(s) 111, 114, 356
GlaI GCGC 1 cut(s) 6
GluI GCNGC 3 cut(s) 111, 114, 356
GsuI CTGGAG 1 cut(s) 199
HaeII RGCGCY 1 cut(s) 8
HaeIII GGCC 3 cut(s) 80, 104, 224
HapII CCGG 4 cut(s) 81, 105, 117, 360
HhaI GCGC 1 cut(s) 7
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 3 cut(s) 229, 238, 380
Hin6I GCGC 1 cut(s) 5
HinP1I GCGC 1 cut(s) 5
HinfI GANTC 3 cut(s) 210, 231, 278
HpaII CCGG 4 cut(s) 81, 105, 117, 360
HphI GGTGA 3 cut(s) 355, 374, 415
Hpy166II GTNNAC 2 cut(s) 196, 414
Hpy188I TCNGA 2 cut(s) 209, 388
Hpy188III TCNNGA 5 cut(s) 184, 235, 262, 334, 423
Hpy8I GTNNAC 2 cut(s) 196, 414
HpyF10VI GCNNNNNNNGC 6 cut(s) 11, 53, 110, 364, 373, 377
HpyF3I CTNAG 4 cut(s) 15, 387, 396, 408
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 3 cut(s) 229, 238, 380
HspAI GCGC 1 cut(s) 5
KasI GGCGCC 1 cut(s) 4
Kzo9I GATC 3 cut(s) 137, 165, 330
LguI GCTCTTC 1 cut(s) 140
LmnI GCTCC 2 cut(s) 96, 107
Lsp1109I GCAGC 1 cut(s) 342
MaeIII GTNAC 1 cut(s) 403
MalI GATC 3 cut(s) 139, 167, 332
MboI GATC 3 cut(s) 137, 165, 330
MboII GAAGA 9 cut(s) 31, 79, 97, 139, 142, 145, 154, 157, 250
MflI RGATCY 2 cut(s) 165, 330
MhlI GDGCHC 1 cut(s) 369
Mly113I GGCGCC 1 cut(s) 5
MlyI GAGTC 1 cut(s) 287
MnlI CCTC 3 cut(s) 94, 318, 382
MroXI GAANNNNTTC 1 cut(s) 242
MspI CCGG 4 cut(s) 81, 105, 117, 360
MspR9I CCNGG 5 cut(s) 82, 275, 286, 295, 360
MvaI CCWGG 3 cut(s) 275, 286, 295
MwoI GCNNNNNNNGC 6 cut(s) 11, 53, 110, 364, 373, 377
NarI GGCGCC 1 cut(s) 5
NciI CCSGG 2 cut(s) 82, 360
NcoI CCATGG 1 cut(s) 225
NdeII GATC 3 cut(s) 137, 165, 330
NlaIII CATG 3 cut(s) 229, 238, 380
NlaIV GGNNCC 2 cut(s) 6, 109
NmeAIII GCCGAG 1 cut(s) 88
NmuCI GTSAC 1 cut(s) 403
NspI RCATGY 1 cut(s) 380
PaeI GCATGC 1 cut(s) 380
PagI TCATGA 1 cut(s) 234
PciSI GCTCTTC 1 cut(s) 140
PcsI WCGNNNNNNNCGW 1 cut(s) 258
PdmI GAANNNNTTC 1 cut(s) 242
PfeI GAWTC 2 cut(s) 210, 231
PkrI GCNGC 3 cut(s) 112, 115, 357
PleI GAGTC 1 cut(s) 286
PluTI GGCGCC 1 cut(s) 8
PpsI GAGTC 1 cut(s) 286
PpuMI RGGWCCY 1 cut(s) 181
Psp124BI GAGCTC 1 cut(s) 369
Psp5II RGGWCCY 1 cut(s) 181
Psp6I CCWGG 3 cut(s) 273, 284, 293
PspGI CCWGG 3 cut(s) 273, 284, 293
PspN4I GGNNCC 2 cut(s) 6, 109
PspPI GGNCC 2 cut(s) 181, 193
PspPPI RGGWCCY 1 cut(s) 181
PsuI RGATCY 2 cut(s) 165, 330
RsaI GTAC 2 cut(s) 299, 394
RsaNI GTAC 2 cut(s) 298, 393
SacI GAGCTC 1 cut(s) 369
SapI GCTCTTC 1 cut(s) 140
SatI GCNGC 3 cut(s) 111, 114, 356
Sau3AI GATC 3 cut(s) 137, 165, 330
Sau96I GGNCC 2 cut(s) 181, 193
SchI GAGTC 1 cut(s) 287
ScrFI CCNGG 5 cut(s) 82, 275, 286, 295, 360
SduI GDGCHC 1 cut(s) 369
SfoI GGCGCC 1 cut(s) 6
SinI GGWCC 2 cut(s) 181, 193
SphI GCATGC 1 cut(s) 380
SsiI CCGC 8 cut(s) 27, 39, 60, 111, 114, 341, 380, 401
SspDI GGCGCC 1 cut(s) 4
SstI GAGCTC 1 cut(s) 369
StyD4I CCNGG 5 cut(s) 80, 273, 284, 293, 358
StyI CCWWGG 2 cut(s) 75, 225
TaqI TCGA 3 cut(s) 140, 261, 323
TauI GCSGC 2 cut(s) 113, 116
TfiI GAWTC 2 cut(s) 210, 231
TseFI GTSAC 1 cut(s) 403
TseI GCWGC 1 cut(s) 355
Tsp45I GTSAC 1 cut(s) 403
TspDTI ATGAA 2 cut(s) 235, 251
VpaK11BI GGWCC 2 cut(s) 181, 193
XagI CCTNNNNNAGG 1 cut(s) 188
XceI RCATGY 1 cut(s) 380
XcmI CCANNNNNNNNNTGG 1 cut(s) 223
XmnI GAANNNNTTC 1 cut(s) 242
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.