Rh5CG453200

histone H2B.1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0000578
Physical Location & Seq
Forward (+)
63115390 .. 63119155
3766 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG453200.1

Sequence Viewer

Length: 447 bp
ATGGCGCCCAAGGCCGAGAAGAAGCCCGCCGAGAAGAAGCCGGCAGAGGAGAAGAAGTCCACCGTCGCCGAGAAGACCCCAGCCGAGAAGAAGCCCAAGGCCGGCAAGAAGCTCCCCAAGGAGGCCGGAGCTGCCGCCGGAGACAAGAAGAAGAAGAGGAACAAGAAGAGCGTAGAGACCTACAAGATCTACATCTTCAAGGTCCTCAAGCAGGTCCACCCTGACATCGGGATCTCCAGCAAGGCCATGGGCATCATGAACAGCTTCATCAACGACATCTTTGAGAAGCTCGCTCAGGAGTCGTCGAGGCTTGCGAGGTACAACAAGAAGCCGACGATTACTTCTCGGGAGATCCAGACTGCTGTCAGGCTTGTTCTTCCCGGTGAATTGGCTAAGCACGCTGTTTCTGAGGGTACAAAGGCTGTGACCAAGTTCACAAGCTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05034 ko05203 ko05322 map05034 map05203 map05322
Pfam Domains
Protein Families
Histone

Protein Analysis

148

Amino Acids

16.28

Weight (kDa)

10.08

Isoelectric Point (pI)

28.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Histone PF00125 48 - 124 2.4e-20 Core histone H2A/H2B/H3/H4 domain
Histone_ABTB PF26281 78 - 147 4.6e-08 Histone domain in ABTB family protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000578)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G45980 AT3G46030 AT5G59910
fragaria_vesca FvH4_3g35121 FvH4_3g35121 FvH4_3g35121 FvH4_3g35180 FvH4_3g35380 FvH4_3g35390 FvH4_3g35440 FvH4_3g40690
malus_domestica MD07G1116500.v1.1 MD11G1112800.v1.1 MD11G1144100.v1.1
prunus_persica Prupe.6G082000_v2.0.a1 Prupe.6G082700_v2.0.a1 Prupe.6G082700_v2.0.a1 Prupe.6G082700_v2.0.a1 Prupe.6G082700_v2.0.a1 Prupe.6G082700_v2.0.a1 Prupe.6G082700_v2.0.a1 Prupe.6G083500_v2.0.a1
pyrus_communis pycom03g08040 pycom11g09350
rosa_chinensis RchiOBHm_Chr5g0063141 RchiOBHm_Chr5g0063151 RchiOBHm_Chr5g0063491 RchiOBHm_Chr5g0063501 RchiOBHm_Chr5g0063561 RchiOBHm_Chr5g0073451
rosa_laevigata RLG00000035607 RLG00000035610 RLG00000035611 RLG00000035640 RLG00000035650 RLG00000035655
rosa_multiflora Rmu_sc0005045.1_g000027 Rmu_sc0005045.1_g000029 Rmu_sc0005045.1_g000036 Rmu_sc0016394.1_g000001 Rmu_sc0021593.1_g000004 Rmu_ssc0000309.1_g000057 Rmu_ssc0000309.1_g000058
rosa_roxburghii Rroxscaffold_1G00007800 Rroxscaffold_1G00017210 Rroxscaffold_1G00017490 Rroxscaffold_1G00017500
rosa_rugosa Rorug05G0354400 Rorug05G0354600 Rorug05G0354700 Rorug05G0357700 Rorug05G0357800 Rorug05G0358300 Rorug05G0423500
rosa_samantha Rh5AG415000 Rh5AG416900 Rh5AG480900 Rh5BG429700 Rh5BG430000 Rh5BG432000 Rh5BG432200 Rh5BG432500 Rh5BG501700 Rh5CG453200 Rh5CG453500 Rh5CG455300 Rh5CG455600 Rh5CG455700 Rh5CG524800 Rh5DG443200 Rh5DG443400 Rh5DG443500 Rh5DG445400 Rh5DG445600 Rh5DG445900 Rh5DG514000
rosa_wichuraiana Rw5G038990 Rw5G039010 Rw5G039160 Rw5G039180 Rw5G044680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 202
AccB1I GGYRCC 1 cut(s) 4
AciI CCGC 2 cut(s) 27, 135
AclWI GGATC 2 cut(s) 239, 346
AcyI GRCGYC 1 cut(s) 5
AfaI GTAC 2 cut(s) 320, 415
AfiI CCNNNNNNNGG 4 cut(s) 101, 121, 211, 227
AgsI TTSAA 1 cut(s) 199
AluBI AGCT 5 cut(s) 112, 131, 264, 289, 441
AluI AGCT 5 cut(s) 112, 131, 264, 289, 441
Alw26I GTCTC 2 cut(s) 135, 170
AlwI GGATC 2 cut(s) 239, 346
Ama87I CYCGRG 1 cut(s) 345
AoxI GGCC 4 cut(s) 12, 99, 123, 243
ApeKI GCWGC 1 cut(s) 131
Asp700I GAANNNNTTC 1 cut(s) 263
AspLEI GCGC 1 cut(s) 7
AspS9I GGNCC 2 cut(s) 202, 214
AsuC2I CCSGG 1 cut(s) 381
AsuHPI GGTGA 1 cut(s) 395
AvaI CYCGRG 1 cut(s) 345
AvaII GGWCC 2 cut(s) 202, 214
BanI GGYRCC 1 cut(s) 4
BbsI GAAGAC 1 cut(s) 80
BbvI GCAGC 1 cut(s) 118
BcnI CCSGG 1 cut(s) 381
BcoDI GTCTC 2 cut(s) 135, 170
BfoI RGCGCY 1 cut(s) 8
BfuAI ACCTGC 1 cut(s) 202
BglII AGATCT 1 cut(s) 186
BisI GCNGC 2 cut(s) 132, 135
BlpI GCTNAGC 1 cut(s) 393
BlsI GCNGC 2 cut(s) 133, 136
Bme1390I CCNGG 1 cut(s) 381
Bme18I GGWCC 2 cut(s) 202, 214
BmeT110I CYCGRG 1 cut(s) 345
BmgT120I GGNCC 2 cut(s) 202, 214
BmiI GGNNCC 1 cut(s) 6
BmrFI CCNGG 1 cut(s) 381
BmsI GCATC 1 cut(s) 261
BoxI GACNNNNGTC 1 cut(s) 362
BpiI GAAGAC 1 cut(s) 80
BpmI CTGGAG 1 cut(s) 220
Bpu10I CCTNAGC 1 cut(s) 294
Bpu1102I GCTNAGC 1 cut(s) 393
BpuEI CTTGAG 1 cut(s) 191
BpuMI CCSGG 1 cut(s) 381
BsaBI GATNNNNATC 1 cut(s) 191
BsaHI GRCGYC 1 cut(s) 5
BsaI GGTCTC 1 cut(s) 170
BsaJI CCNNGG 4 cut(s) 9, 96, 117, 246
BsaXI ACNNNNNCTCC 2 cut(s) 41, 71
Bsc4I CCNNNNNNNGG 4 cut(s) 101, 121, 211, 227
Bse118I RCCGGY 2 cut(s) 40, 101
Bse8I GATNNNNATC 1 cut(s) 191
BseDI CCNNGG 4 cut(s) 9, 96, 117, 246
BseJI GATNNNNATC 1 cut(s) 191
BseLI CCNNNNNNNGG 4 cut(s) 101, 121, 211, 227
BseMII CTCAG 2 cut(s) 308, 399
BseRI GAGGAG 1 cut(s) 62
BseXI GCAGC 1 cut(s) 118
BseYI CCCAGC 1 cut(s) 79
BshFI GGCC 4 cut(s) 14, 101, 125, 245
BshNI GGYRCC 1 cut(s) 4
BsiHKCI CYCGRG 1 cut(s) 345
BsiSI CCGG 5 cut(s) 41, 102, 126, 138, 381
BslI CCNNNNNNNGG 4 cut(s) 101, 121, 211, 227
BsmAI GTCTC 2 cut(s) 135, 170
BsnI GGCC 4 cut(s) 14, 101, 125, 245
Bso31I GGTCTC 1 cut(s) 170
BsoBI CYCGRG 1 cut(s) 345
Bsp143I GATC 3 cut(s) 186, 231, 351
Bsp1720I GCTNAGC 1 cut(s) 393
Bsp19I CCATGG 1 cut(s) 246
BspACI CCGC 2 cut(s) 27, 135
BspANI GGCC 4 cut(s) 14, 101, 125, 245
BspCNI CTCAG 2 cut(s) 307, 400
BspHI TCATGA 1 cut(s) 255
BspLI GGNNCC 1 cut(s) 6
BspMI ACCTGC 1 cut(s) 202
BspPI GGATC 2 cut(s) 239, 346
BspQI GCTCTTC 1 cut(s) 161
BspT107I GGYRCC 1 cut(s) 4
BspTNI GGTCTC 1 cut(s) 170
BsrFI RCCGGY 2 cut(s) 40, 101
BssAI RCCGGY 2 cut(s) 40, 101
BssECI CCNNGG 4 cut(s) 9, 96, 117, 246
BssMI GATC 3 cut(s) 186, 231, 351
BssNI GRCGYC 1 cut(s) 5
BssT1I CCWWGG 4 cut(s) 9, 96, 117, 246
Bst4CI ACNGT 1 cut(s) 64
Bst6I CTCTTC 2 cut(s) 149, 161
BstACI GRCGYC 1 cut(s) 5
BstC8I GCNNGC 6 cut(s) 27, 42, 103, 291, 312, 399
BstDEI CTNAG 3 cut(s) 294, 393, 408
BstDSI CCRYGG 1 cut(s) 246
BstENI CCTNNNNNAGG 1 cut(s) 209
BstH2I RGCGCY 1 cut(s) 8
BstHHI GCGC 1 cut(s) 7
BstKTI GATC 3 cut(s) 189, 234, 354
BstMAI GTCTC 2 cut(s) 135, 170
BstMBI GATC 3 cut(s) 186, 231, 351
BstMWI GCNNNNNNNGC 3 cut(s) 11, 131, 398
BstPAI GACNNNNGTC 1 cut(s) 362
BstSCI CCNGG 1 cut(s) 379
BstV1I GCAGC 1 cut(s) 118
BstV2I GAAGAC 1 cut(s) 80
BstX2I RGATCY 3 cut(s) 186, 231, 351
BstYI RGATCY 3 cut(s) 186, 231, 351
BsuRI GGCC 4 cut(s) 14, 101, 125, 245
BtgI CCRYGG 1 cut(s) 246
BveI ACCTGC 1 cut(s) 202
Cac8I GCNNGC 6 cut(s) 27, 42, 103, 291, 312, 399
CciI TCATGA 1 cut(s) 255
CfoI GCGC 1 cut(s) 7
Cfr10I RCCGGY 2 cut(s) 40, 101
Cfr13I GGNCC 2 cut(s) 202, 214
Csp6I GTAC 2 cut(s) 319, 414
CviAII CATG 2 cut(s) 247, 256
CviQI GTAC 2 cut(s) 319, 414
DdeI CTNAG 3 cut(s) 294, 393, 408
DinI GGCGCC 1 cut(s) 6
DpnI GATC 3 cut(s) 188, 233, 353
DpnII GATC 3 cut(s) 186, 231, 351
Eam1104I CTCTTC 2 cut(s) 149, 161
EarI CTCTTC 2 cut(s) 149, 161
Eco130I CCWWGG 4 cut(s) 9, 96, 117, 246
Eco31I GGTCTC 1 cut(s) 170
Eco47I GGWCC 2 cut(s) 202, 214
Eco88I CYCGRG 1 cut(s) 345
EcoNI CCTNNNNNAGG 1 cut(s) 209
EcoO109I RGGNCCY 1 cut(s) 202
EcoT14I CCWWGG 4 cut(s) 9, 96, 117, 246
EgeI GGCGCC 1 cut(s) 6
EheI GGCGCC 1 cut(s) 6
ErhI CCWWGG 4 cut(s) 9, 96, 117, 246
FaeI CATG 2 cut(s) 250, 259
FaiI YATR 2 cut(s) 248, 257
FatI CATG 2 cut(s) 246, 255
FauI CCCGC 1 cut(s) 34
Fnu4HI GCNGC 2 cut(s) 132, 135
Fsp4HI GCNGC 2 cut(s) 132, 135
GlaI GCGC 1 cut(s) 6
GluI GCNGC 2 cut(s) 132, 135
GsaI CCCAGC 1 cut(s) 83
GsuI CTGGAG 1 cut(s) 220
HaeII RGCGCY 1 cut(s) 8
HaeIII GGCC 4 cut(s) 14, 101, 125, 245
HapII CCGG 5 cut(s) 41, 102, 126, 138, 381
HhaI GCGC 1 cut(s) 7
Hin1I GRCGYC 1 cut(s) 5
Hin1II CATG 2 cut(s) 250, 259
Hin6I GCGC 1 cut(s) 5
HinP1I GCGC 1 cut(s) 5
HinfI GANTC 1 cut(s) 299
HpaII CCGG 5 cut(s) 41, 102, 126, 138, 381
HphI GGTGA 1 cut(s) 395
Hpy166II GTNNAC 3 cut(s) 60, 217, 435
Hpy188I TCNGA 1 cut(s) 409
Hpy188III TCNNGA 6 cut(s) 229, 256, 296, 347, 355, 444
Hpy8I GTNNAC 3 cut(s) 60, 217, 435
Hpy99I CGWCG 3 cut(s) 68, 307, 337
HpyCH4III ACNGT 1 cut(s) 64
HpyF10VI GCNNNNNNNGC 3 cut(s) 11, 131, 398
HpyF3I CTNAG 3 cut(s) 294, 393, 408
Hsp92I GRCGYC 1 cut(s) 5
Hsp92II CATG 2 cut(s) 250, 259
HspAI GCGC 1 cut(s) 5
KasI GGCGCC 1 cut(s) 4
KroI GCCGGC 2 cut(s) 40, 101
KroNI GCCGGC 2 cut(s) 42, 103
Kzo9I GATC 3 cut(s) 186, 231, 351
LguI GCTCTTC 1 cut(s) 161
LmnI GCTCC 2 cut(s) 117, 128
Lsp1109I GCAGC 1 cut(s) 118
LweI GCATC 1 cut(s) 261
MaeIII GTNAC 1 cut(s) 424
MalI GATC 3 cut(s) 188, 233, 353
MboI GATC 3 cut(s) 186, 231, 351
MflI RGATCY 3 cut(s) 186, 231, 351
MluCI AATT 1 cut(s) 386
Mly113I GGCGCC 1 cut(s) 5
MlyI GAGTC 1 cut(s) 308
MnlI CCTC 7 cut(s) 40, 115, 150, 215, 300, 309, 403
MroNI GCCGGC 2 cut(s) 40, 101
MroXI GAANNNNTTC 1 cut(s) 263
MspI CCGG 5 cut(s) 41, 102, 126, 138, 381
MspR9I CCNGG 1 cut(s) 381
MwoI GCNNNNNNNGC 3 cut(s) 11, 131, 398
NaeI GCCGGC 2 cut(s) 42, 103
NarI GGCGCC 1 cut(s) 5
NciI CCSGG 1 cut(s) 381
NcoI CCATGG 1 cut(s) 246
NdeII GATC 3 cut(s) 186, 231, 351
NgoMIV GCCGGC 2 cut(s) 40, 101
NlaIII CATG 2 cut(s) 250, 259
NlaIV GGNNCC 1 cut(s) 6
NmeAIII GCCGAG 4 cut(s) 40, 55, 94, 109
NmuCI GTSAC 1 cut(s) 424
PagI TCATGA 1 cut(s) 255
PciSI GCTCTTC 1 cut(s) 161
PcsI WCGNNNNNNNCGW 1 cut(s) 311
PdiI GCCGGC 2 cut(s) 42, 103
PdmI GAANNNNTTC 1 cut(s) 263
PkrI GCNGC 2 cut(s) 133, 136
PleI GAGTC 1 cut(s) 307
PluTI GGCGCC 1 cut(s) 8
PpsI GAGTC 1 cut(s) 307
PpuMI RGGWCCY 1 cut(s) 202
PshAI GACNNNNGTC 1 cut(s) 362
Psp5II RGGWCCY 1 cut(s) 202
PspFI CCCAGC 1 cut(s) 79
PspN4I GGNNCC 1 cut(s) 6
PspPI GGNCC 2 cut(s) 202, 214
PspPPI RGGWCCY 1 cut(s) 202
PsuI RGATCY 3 cut(s) 186, 231, 351
RsaI GTAC 2 cut(s) 320, 415
RsaNI GTAC 2 cut(s) 319, 414
SapI GCTCTTC 1 cut(s) 161
SatI GCNGC 2 cut(s) 132, 135
Sau3AI GATC 3 cut(s) 186, 231, 351
Sau96I GGNCC 2 cut(s) 202, 214
SchI GAGTC 1 cut(s) 308
ScrFI CCNGG 1 cut(s) 381
SetI ASST 9 cut(s) 114, 133, 182, 204, 216, 266, 291, 320, 443
SfaNI GCATC 1 cut(s) 261
SfoI GGCGCC 1 cut(s) 6
SinI GGWCC 2 cut(s) 202, 214
SmlI CTYRAG 1 cut(s) 206
SmoI CTYRAG 1 cut(s) 206
Sse9I AATT 1 cut(s) 386
SsiI CCGC 2 cut(s) 27, 135
SspDI GGCGCC 1 cut(s) 4
StyD4I CCNGG 1 cut(s) 379
StyI CCWWGG 4 cut(s) 9, 96, 117, 246
TaaI ACNGT 1 cut(s) 64
TaqI TCGA 1 cut(s) 305
TasI AATT 1 cut(s) 386
TauI GCSGC 1 cut(s) 137
TseFI GTSAC 1 cut(s) 424
TseI GCWGC 1 cut(s) 131
Tsp45I GTSAC 1 cut(s) 424
TspDTI ATGAA 2 cut(s) 256, 272
VpaK11BI GGWCC 2 cut(s) 202, 214
XagI CCTNNNNNAGG 1 cut(s) 209
XcmI CCANNNNNNNNNTGG 1 cut(s) 244
XmnI GAANNNNTTC 1 cut(s) 263
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.