pycom03g08040

histone H2B.1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0000578
Physical Location & Seq
Reverse (-)
6428519 .. 6429429
911 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g08040.1

Sequence Viewer

Length: 591 bp
ATGATTAGTAAAATATTATTTGATTTGAAAATTTTATTTTTTACAAATCATTACACTTTTTATCCCACAAATTCCAAGTCCGCCCAATTTTCCTCTCTTCTCAAAGTTTCCGTTTGCTTACCGAGAAAGAAAAGCCAAGAAGATATGGCGCCCAAAGCCGAGAAGAAGCCCGCCGAGAAGAAGCCCGCGGAGGAGAAGAAGTCCGCCGTGGCGGAGAAAGCCCCCGCCGAGAAGAAGCCCAAGGCCGGGAAGAAGCTCCCGAAGGAGGCCGGTGCCGCCGCCGGAGACAAGAAGAAGAAGAGATCGAAGAAGAGCGTGGAGACCTACAAGATCTACATCTTCAAGGTGCTGAAGCAGGTCCATCCTGACATCGGGATCTCCAGCAAGGCCATGGGAATCATGAACAGCTTCATCAACGACATCTTCGAGAAGCTCGCCCAGGAGTCATCCAGGCTCGCGAGGTACAACAAGAAGCCGACGATTACTTCCCGGGAGATCCAAACTGCTGTGAGATTGGTGCTGCCTGGTGAGCTTGCTAAGCATGCGGTGTCTGAGGGGACTAAGGCGGTGACTAAGTTTACTAGCTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko05034 ko05203 ko05322 map05034 map05203 map05322
Pfam Domains
Protein Families
Histone

Protein Analysis

197

Amino Acids

21.79

Weight (kDa)

10.02

Isoelectric Point (pI)

33.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Histone PF00125 96 - 172 2.2e-20 Core histone H2A/H2B/H3/H4 domain
Histone_ABTB PF26281 126 - 194 9.1e-08 Histone domain in ABTB family protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000578)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G45980 AT3G46030 AT5G59910
fragaria_vesca FvH4_3g35121 FvH4_3g35121 FvH4_3g35121 FvH4_3g35180 FvH4_3g35380 FvH4_3g35390 FvH4_3g35440 FvH4_3g40690
malus_domestica MD07G1116500.v1.1 MD11G1112800.v1.1 MD11G1144100.v1.1
prunus_persica Prupe.6G082000_v2.0.a1 Prupe.6G082700_v2.0.a1 Prupe.6G082700_v2.0.a1 Prupe.6G082700_v2.0.a1 Prupe.6G082700_v2.0.a1 Prupe.6G082700_v2.0.a1 Prupe.6G082700_v2.0.a1 Prupe.6G083500_v2.0.a1
pyrus_communis pycom03g08040 pycom11g09350
rosa_chinensis RchiOBHm_Chr5g0063141 RchiOBHm_Chr5g0063151 RchiOBHm_Chr5g0063491 RchiOBHm_Chr5g0063501 RchiOBHm_Chr5g0063561 RchiOBHm_Chr5g0073451
rosa_laevigata RLG00000035607 RLG00000035610 RLG00000035611 RLG00000035640 RLG00000035650 RLG00000035655
rosa_multiflora Rmu_sc0005045.1_g000027 Rmu_sc0005045.1_g000029 Rmu_sc0005045.1_g000036 Rmu_sc0016394.1_g000001 Rmu_sc0021593.1_g000004 Rmu_ssc0000309.1_g000057 Rmu_ssc0000309.1_g000058
rosa_roxburghii Rroxscaffold_1G00007800 Rroxscaffold_1G00017210 Rroxscaffold_1G00017490 Rroxscaffold_1G00017500
rosa_rugosa Rorug05G0354400 Rorug05G0354600 Rorug05G0354700 Rorug05G0357700 Rorug05G0357800 Rorug05G0358300 Rorug05G0423500
rosa_samantha Rh5AG415000 Rh5AG416900 Rh5AG480900 Rh5BG429700 Rh5BG430000 Rh5BG432000 Rh5BG432200 Rh5BG432500 Rh5BG501700 Rh5CG453200 Rh5CG453500 Rh5CG455300 Rh5CG455600 Rh5CG455700 Rh5CG524800 Rh5DG443200 Rh5DG443400 Rh5DG443500 Rh5DG445400 Rh5DG445600 Rh5DG445900 Rh5DG514000
rosa_wichuraiana Rw5G038990 Rw5G039010 Rw5G039160 Rw5G039180 Rw5G044680

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 346
AccB1I GGYRCC 2 cut(s) 148, 272
AccII CGCG 2 cut(s) 188, 458
AclWI GGATC 2 cut(s) 383, 490
AcsI RAATTY 2 cut(s) 30, 70
AcuI CTGAAG 1 cut(s) 371
AcyI GRCGYC 1 cut(s) 149
AfaI GTAC 1 cut(s) 464
AfiI CCNNNNNNNGG 4 cut(s) 245, 246, 265, 371
AgsI TTSAA 2 cut(s) 28, 343
AjnI CCWGG 3 cut(s) 438, 449, 523
AluBI AGCT 5 cut(s) 256, 408, 433, 532, 585
AluI AGCT 5 cut(s) 256, 408, 433, 532, 585
Alw26I GTCTC 2 cut(s) 279, 314
AlwI GGATC 2 cut(s) 383, 490
Ama87I CYCGRG 1 cut(s) 489
AoxI GGCC 3 cut(s) 243, 267, 387
ApeKI GCWGC 1 cut(s) 520
ApoI RAATTY 2 cut(s) 30, 70
ArsI GACNNNNNNTTYG 2 cut(s) 62, 94
Asp700I GAANNNNTTC 1 cut(s) 407
AspLEI GCGC 1 cut(s) 151
AspS9I GGNCC 1 cut(s) 358
AsuC2I CCSGG 3 cut(s) 247, 490, 491
AsuHPI GGTGA 2 cut(s) 539, 580
AvaI CYCGRG 1 cut(s) 489
AvaII GGWCC 1 cut(s) 358
BanI GGYRCC 2 cut(s) 148, 272
BbvI GCAGC 1 cut(s) 507
BccI CCATC 1 cut(s) 369
BceAI ACGGC 1 cut(s) 191
BciT130I CCWGG 3 cut(s) 440, 451, 525
BcnI CCSGG 3 cut(s) 247, 490, 491
BcoDI GTCTC 2 cut(s) 279, 314
BfaI CTAG 1 cut(s) 582
BfoI RGCGCY 1 cut(s) 152
BfuAI ACCTGC 1 cut(s) 346
BglII AGATCT 1 cut(s) 330
BisI GCNGC 3 cut(s) 276, 279, 521
BlpI GCTNAGC 1 cut(s) 537
BlsI GCNGC 3 cut(s) 277, 280, 522
Bme1390I CCNGG 6 cut(s) 247, 440, 451, 490, 491, 525
Bme18I GGWCC 1 cut(s) 358
BmeT110I CYCGRG 1 cut(s) 489
BmgT120I GGNCC 1 cut(s) 358
BmiI GGNNCC 2 cut(s) 150, 274
BmrFI CCNGG 6 cut(s) 247, 440, 451, 490, 491, 525
BpmI CTGGAG 1 cut(s) 364
Bpu1102I GCTNAGC 1 cut(s) 537
BpuMI CCSGG 3 cut(s) 247, 490, 491
BsaBI GATNNNNATC 1 cut(s) 335
BsaHI GRCGYC 1 cut(s) 149
BsaI GGTCTC 1 cut(s) 314
BsaJI CCNNGG 6 cut(s) 186, 207, 240, 390, 438, 489
BsaXI ACNNNNNCTCC 2 cut(s) 185, 215
Bsc4I CCNNNNNNNGG 4 cut(s) 245, 246, 265, 371
Bse118I RCCGGY 1 cut(s) 269
Bse8I GATNNNNATC 1 cut(s) 335
BseBI CCWGG 3 cut(s) 440, 451, 525
BseDI CCNNGG 6 cut(s) 186, 207, 240, 390, 438, 489
BseGI GGATG 2 cut(s) 361, 446
BseJI GATNNNNATC 1 cut(s) 335
BseLI CCNNNNNNNGG 4 cut(s) 245, 246, 265, 371
BseMII CTCAG 1 cut(s) 543
BseRI GAGGAG 1 cut(s) 206
BseXI GCAGC 1 cut(s) 507
Bsh1236I CGCG 2 cut(s) 188, 458
BshFI GGCC 3 cut(s) 245, 269, 389
BshNI GGYRCC 2 cut(s) 148, 272
BsiHKCI CYCGRG 1 cut(s) 489
BsiSI CCGG 4 cut(s) 246, 270, 282, 490
BslFI GGGAC 1 cut(s) 571
BslI CCNNNNNNNGG 4 cut(s) 245, 246, 265, 371
BsmAI GTCTC 2 cut(s) 279, 314
BsmFI GGGAC 1 cut(s) 571
BsnI GGCC 3 cut(s) 245, 269, 389
Bso31I GGTCTC 1 cut(s) 314
BsoBI CYCGRG 1 cut(s) 489
Bsp143I GATC 4 cut(s) 302, 330, 375, 495
Bsp1720I GCTNAGC 1 cut(s) 537
Bsp19I CCATGG 1 cut(s) 390
Bsp68I TCGCGA 1 cut(s) 458
BspANI GGCC 3 cut(s) 245, 269, 389
BspCNI CTCAG 1 cut(s) 544
BspFNI CGCG 2 cut(s) 188, 458
BspHI TCATGA 1 cut(s) 399
BspLI GGNNCC 2 cut(s) 150, 274
BspMI ACCTGC 1 cut(s) 346
BspPI GGATC 2 cut(s) 383, 490
BspQI GCTCTTC 1 cut(s) 305
BspT107I GGYRCC 2 cut(s) 148, 272
BspTNI GGTCTC 1 cut(s) 314
BsrFI RCCGGY 1 cut(s) 269
BssAI RCCGGY 1 cut(s) 269
BssECI CCNNGG 6 cut(s) 186, 207, 240, 390, 438, 489
BssMI GATC 4 cut(s) 302, 330, 375, 495
BssNI GRCGYC 1 cut(s) 149
BssT1I CCWWGG 2 cut(s) 240, 390
Bst2UI CCWGG 3 cut(s) 440, 451, 525
Bst6I CTCTTC 3 cut(s) 102, 293, 305
BstACI GRCGYC 1 cut(s) 149
BstC8I GCNNGC 6 cut(s) 171, 186, 435, 456, 534, 543
BstDEI CTNAG 4 cut(s) 537, 552, 561, 573
BstDSI CCRYGG 3 cut(s) 186, 207, 390
BstF5I GGATG 2 cut(s) 361, 446
BstFNI CGCG 2 cut(s) 188, 458
BstH2I RGCGCY 1 cut(s) 152
BstHHI GCGC 1 cut(s) 151
BstKTI GATC 4 cut(s) 305, 333, 378, 498
BstMAI GTCTC 2 cut(s) 279, 314
BstMBI GATC 4 cut(s) 302, 330, 375, 495
BstMWI GCNNNNNNNGC 6 cut(s) 155, 218, 275, 529, 538, 542
BstNI CCWGG 3 cut(s) 440, 451, 525
BstNSI RCATGY 1 cut(s) 545
BstSCI CCNGG 6 cut(s) 245, 438, 449, 488, 489, 523
BstUI CGCG 2 cut(s) 188, 458
BstV1I GCAGC 1 cut(s) 507
BstX2I RGATCY 3 cut(s) 330, 375, 495
BstYI RGATCY 3 cut(s) 330, 375, 495
BsuRI GGCC 3 cut(s) 245, 269, 389
BtgI CCRYGG 3 cut(s) 186, 207, 390
BtsCI GGATG 2 cut(s) 361, 446
BtuMI TCGCGA 1 cut(s) 458
BveI ACCTGC 1 cut(s) 346
Cac8I GCNNGC 6 cut(s) 171, 186, 435, 456, 534, 543
CciI TCATGA 1 cut(s) 399
CfoI GCGC 1 cut(s) 151
Cfr10I RCCGGY 1 cut(s) 269
Cfr13I GGNCC 1 cut(s) 358
Cfr42I CCGCGG 1 cut(s) 189
Cfr9I CCCGGG 1 cut(s) 489
Csp6I GTAC 1 cut(s) 463
CviAII CATG 3 cut(s) 391, 400, 542
CviQI GTAC 1 cut(s) 463
DdeI CTNAG 4 cut(s) 537, 552, 561, 573
DinI GGCGCC 1 cut(s) 150
DpnI GATC 4 cut(s) 304, 332, 377, 497
DpnII GATC 4 cut(s) 302, 330, 375, 495
Eam1104I CTCTTC 3 cut(s) 102, 293, 305
EarI CTCTTC 3 cut(s) 102, 293, 305
EciI GGCGGA 3 cut(s) 70, 193, 227
Eco130I CCWWGG 2 cut(s) 240, 390
Eco31I GGTCTC 1 cut(s) 314
Eco47I GGWCC 1 cut(s) 358
Eco57I CTGAAG 1 cut(s) 371
Eco88I CYCGRG 1 cut(s) 489
EcoRII CCWGG 3 cut(s) 438, 449, 523
EcoT14I CCWWGG 2 cut(s) 240, 390
EgeI GGCGCC 1 cut(s) 150
EheI GGCGCC 1 cut(s) 150
ErhI CCWWGG 2 cut(s) 240, 390
FaeI CATG 3 cut(s) 394, 403, 545
FaiI YATR 4 cut(s) 146, 392, 401, 543
FaqI GGGAC 1 cut(s) 571
FatI CATG 3 cut(s) 390, 399, 541
FauI CCCGC 3 cut(s) 178, 193, 232
Fnu4HI GCNGC 3 cut(s) 276, 279, 521
FokI GGATG 2 cut(s) 348, 433
Fsp4HI GCNGC 3 cut(s) 276, 279, 521
FspBI CTAG 1 cut(s) 582
GlaI GCGC 1 cut(s) 150
GluI GCNGC 3 cut(s) 276, 279, 521
GsuI CTGGAG 1 cut(s) 364
HaeII RGCGCY 1 cut(s) 152
HaeIII GGCC 3 cut(s) 245, 269, 389
HapII CCGG 4 cut(s) 246, 270, 282, 490
HhaI GCGC 1 cut(s) 151
Hin1I GRCGYC 1 cut(s) 149
Hin1II CATG 3 cut(s) 394, 403, 545
Hin6I GCGC 1 cut(s) 149
HinP1I GCGC 1 cut(s) 149
HinfI GANTC 2 cut(s) 396, 443
HpaII CCGG 4 cut(s) 246, 270, 282, 490
HphI GGTGA 2 cut(s) 539, 580
Hpy166II GTNNAC 1 cut(s) 579
Hpy188I TCNGA 1 cut(s) 553
Hpy188III TCNNGA 7 cut(s) 259, 365, 373, 400, 427, 457, 588
Hpy8I GTNNAC 1 cut(s) 579
Hpy99I CGWCG 1 cut(s) 481
HpyAV CCTTC 1 cut(s) 256
HpyF10VI GCNNNNNNNGC 6 cut(s) 155, 218, 275, 529, 538, 542
HpyF3I CTNAG 4 cut(s) 537, 552, 561, 573
Hsp92I GRCGYC 1 cut(s) 149
Hsp92II CATG 3 cut(s) 394, 403, 545
HspAI GCGC 1 cut(s) 149
KasI GGCGCC 1 cut(s) 148
KspI CCGCGG 1 cut(s) 189
Kzo9I GATC 4 cut(s) 302, 330, 375, 495
LguI GCTCTTC 1 cut(s) 305
LmnI GCTCC 1 cut(s) 261
Lsp1109I GCAGC 1 cut(s) 507
MaeI CTAG 1 cut(s) 582
MaeIII GTNAC 1 cut(s) 568
MalI GATC 4 cut(s) 304, 332, 377, 497
MboI GATC 4 cut(s) 302, 330, 375, 495
MflI RGATCY 3 cut(s) 330, 375, 495
MluCI AATT 3 cut(s) 30, 70, 86
Mly113I GGCGCC 1 cut(s) 149
MlyI GAGTC 1 cut(s) 452
MnlI CCTC 5 cut(s) 103, 184, 259, 453, 547
MroXI GAANNNNTTC 1 cut(s) 407
MspA1I CMGCKG 1 cut(s) 188
MspI CCGG 4 cut(s) 246, 270, 282, 490
MspR9I CCNGG 6 cut(s) 247, 440, 451, 490, 491, 525
MvaI CCWGG 3 cut(s) 440, 451, 525
MvnI CGCG 2 cut(s) 188, 458
MwoI GCNNNNNNNGC 6 cut(s) 155, 218, 275, 529, 538, 542
NarI GGCGCC 1 cut(s) 149
NciI CCSGG 3 cut(s) 247, 490, 491
NcoI CCATGG 1 cut(s) 390
NdeII GATC 4 cut(s) 302, 330, 375, 495
NlaIII CATG 3 cut(s) 394, 403, 545
NlaIV GGNNCC 2 cut(s) 150, 274
NmeAIII GCCGAG 3 cut(s) 184, 199, 253
NmuCI GTSAC 1 cut(s) 568
NruI TCGCGA 1 cut(s) 458
NspI RCATGY 1 cut(s) 545
PaeI GCATGC 1 cut(s) 545
PagI TCATGA 1 cut(s) 399
PciSI GCTCTTC 1 cut(s) 305
PcsI WCGNNNNNNNCGW 1 cut(s) 423
PdmI GAANNNNTTC 1 cut(s) 407
PfeI GAWTC 1 cut(s) 396
PkrI GCNGC 3 cut(s) 277, 280, 522
PleI GAGTC 1 cut(s) 451
PluTI GGCGCC 1 cut(s) 152
PpsI GAGTC 1 cut(s) 451
Psp6I CCWGG 3 cut(s) 438, 449, 523
PspGI CCWGG 3 cut(s) 438, 449, 523
PspN4I GGNNCC 2 cut(s) 150, 274
PspPI GGNCC 1 cut(s) 358
PsuI RGATCY 3 cut(s) 330, 375, 495
RruI TCGCGA 1 cut(s) 458
RsaI GTAC 1 cut(s) 464
RsaNI GTAC 1 cut(s) 463
SacII CCGCGG 1 cut(s) 189
SapI GCTCTTC 1 cut(s) 305
SatI GCNGC 3 cut(s) 276, 279, 521
Sau3AI GATC 4 cut(s) 302, 330, 375, 495
Sau96I GGNCC 1 cut(s) 358
SchI GAGTC 1 cut(s) 452
ScrFI CCNGG 6 cut(s) 247, 440, 451, 490, 491, 525
SetI ASST 9 cut(s) 258, 326, 348, 360, 410, 435, 464, 534, 587
SfoI GGCGCC 1 cut(s) 150
Sfr303I CCGCGG 1 cut(s) 189
SgrBI CCGCGG 1 cut(s) 189
SinI GGWCC 1 cut(s) 358
SmaI CCCGGG 1 cut(s) 491
SphI GCATGC 1 cut(s) 545
Sse9I AATT 3 cut(s) 30, 70, 86
SspDI GGCGCC 1 cut(s) 148
SspI AATATT 1 cut(s) 15
SspMI CTAG 1 cut(s) 582
StyD4I CCNGG 6 cut(s) 245, 438, 449, 488, 489, 523
StyI CCWWGG 2 cut(s) 240, 390
TaqI TCGA 2 cut(s) 305, 426
TasI AATT 3 cut(s) 30, 70, 86
TauI GCSGC 2 cut(s) 278, 281
TfiI GAWTC 1 cut(s) 396
TseFI GTSAC 1 cut(s) 568
TseI GCWGC 1 cut(s) 520
Tsp45I GTSAC 1 cut(s) 568
TspDTI ATGAA 2 cut(s) 400, 416
TspGWI ACGGA 1 cut(s) 100
TspMI CCCGGG 1 cut(s) 489
VpaK11BI GGWCC 1 cut(s) 358
XapI RAATTY 2 cut(s) 30, 70
XceI RCATGY 1 cut(s) 545
XcmI CCANNNNNNNNNTGG 1 cut(s) 388
XmaI CCCGGG 1 cut(s) 489
XmnI GAANNNNTTC 1 cut(s) 407
XspI CTAG 1 cut(s) 582
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.