Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0003551

Physical Location & Seq

Genomic Coordinates Forward (+)

17897506 .. 17899351

Gene Structure 1846 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT3G48340.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1086 bp

ATGAAGAAACTTCTTTTGATATTTCTCTTTTCTCTTGTCATTCTCCAAACTGCTTGTGGATTCGATTACGACGACAAGGAAATAGAAAGCGAAGAGGGCTTATCAACGCTTTATGATAGATGGAGGAGCCACCACTCCGTTCCTAGGAGTCTAAATGAAAGGGAGAAGAGATTCAACGTTTTCAGACACAATGTCATGCATGTCCACAATACCAACAAGAAGAACCGATCCTACAAACTTAAGCTCAACAAGTTTGCAGATTTAACAATTAATGAGTTCAAGAATGCATACACCGGTTCCAACATCAAGCATCACAGAATGTTGCAAGGACCAAAACGCGGCTCAAAACAGTTCATGTATGATCATGAGAATTTATCCAAATTACCGTCCTCCGTTGATTGGAGAAAGAAAGGTGCTGTCACTGAAATCAAGAATCAAGGAAAATGTGGAAGTTGTTGGGCATTCTCTACCGTAGCAGCTGTTGAGGGAATCAACAAGATCAAGACTAACAAACTGGTCTCACTGTCTGAACAAGAACTAGTGGATTGTGATACTAAGCAGAATGAAGGTTGTAACGGAGGTCTTATGGAGATTGCATTTGAATTCATCAAGAAGAACGGTGGTATCACCACTGAAGATAGCTATCCTTATGAAGGTATCGATGGAAAATGCGACGCTTCAAAGGATAACGGCGTGCTTGTGACGATAGATGGGCATGAAGATGTACCCGAAAACGACGAAAATGCTCTTCTTAAGGCTGTTGCAAACCAACCTGTATCTGTTGCAATTGATGCTGGAAGTTCAGATTTCCAGTTTTATTCAGAGGGAGTGTTTACGGGATCTTGTGGAACAGAGCTGAACCATGGTGTGGCAGCGGTCGGGTATGGATCTGAGAGGGGGAAAAAGTATTGGATAGTGAGAAACTCATGGGGAGCTGAATGGGGAGAGGGAGGCTATATAAAGATTGAAAGAGAGATTGATGAACCGGAAGGGCGTTGCGGTATTGCGATGGAGGCATCGTATCCGATCAAGCTATCGTCGTCTAATCCTACCCCGAAAGACGGTGATGTCAAAGATGAGCTCTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0000323 GO:0000325 GO:0002376 GO:0003006 GO:0003674 GO:0003824 GO:0004175 GO:0004197 GO:0005575 GO:0005576 GO:0005615 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005764 GO:0005773 GO:0005783 GO:0005788 GO:0006508 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0007275 GO:0008150 GO:0008152 GO:0008219 GO:0008233 GO:0008234 GO:0009056 GO:0009057 GO:0009505 GO:0009555 GO:0009605 GO:0009607 GO:0009620 GO:0009626 GO:0009791 GO:0009908 GO:0009987 GO:0010623 GO:0012501 GO:0012505 GO:0016787 GO:0019538 GO:0022414 GO:0030154 GO:0030163 GO:0030312 GO:0031974 GO:0032501 GO:0032502 GO:0033554 GO:0034050 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0044421 GO:0044422 GO:0044424 GO:0044432 GO:0044444 GO:0044446 GO:0044464 GO:0045087 GO:0048229 GO:0048367 GO:0048437 GO:0048438 GO:0048443 GO:0048466 GO:0048468 GO:0048608 GO:0048653 GO:0048658 GO:0048731 GO:0048827 GO:0048856 GO:0048869 GO:0050832 GO:0050896 GO:0051603 GO:0051704 GO:0051707 GO:0051716 GO:0061458 GO:0070011 GO:0070013 GO:0071704 GO:0071944 GO:0090567 GO:0098542 GO:0099402 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Inhibitor_I29 Peptidase_C1

Protein Analysis

361

Amino Acids

40.37

Weight (kDa)

5.99

Isoelectric Point (pI)

40.2

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Inhibitor_I29	PF08246	38 - 93	1.2e-12	Cathepsin propeptide inhibitor domain (I29)
Peptidase_C1	PF00112	128 - 343	8.1e-88	Papain family cysteine protease

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003551)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G48340 AT3G48350 AT3G48350 AT5G50260
fragaria_vesca	FvH4_5g06770 FvH4_5g06770
malus_domestica	MD06G1161000.v1.1 MD13G1105500.v1.1 MD14G1166600.v1.1 MD16G1105700.v1.1
prunus_persica	Prupe.1G240900_v2.0.a1 Prupe.5G160900_v2.0.a1
pyrus_communis	pycom06g14380 pycom14g13920
rosa_chinensis	RchiOBHm_Chr4g0432451 RchiOBHm_Chr7g0191791
rosa_laevigata	RLG00000004423 RLG00000006841
rosa_multiflora	Rmu_sc0041316.1_g000001 Rmu_ssc0000421.1_g000001
rosa_roxburghii	Rroxscaffold_3G00263520 Rroxscaffold_5G00373930
rosa_rugosa	Rorug04G0259100.1 Rorug04G0259200.1 Rorug04G0259300 Rorug04G0259400 Rorug06G0511400
rosa_samantha	Rh4AG314400 Rh4BG322200 Rh4CG337300 Rh7AG121500 Rh7BG122900 Rh7CG125800
rosa_wichuraiana	Rw4G027340 Rw7G010330

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1067
AccB7I	CCANNNNNTGG	1 cut(s)	868
AccII	CGCG	1 cut(s)	339
AciI	CCGC	3 cut(s)	339, 875, 999
AclI	AACGTT	1 cut(s)	177
AclWI	GGATC	3 cut(s)	222, 847, 895
AcsI	RAATTY	2 cut(s)	370, 602
AcuI	CTGAAG	1 cut(s)	654
AfaI	GTAC	1 cut(s)	726
AfiI	CCNNNNNNNGG	6 cut(s)	144, 338, 399, 653, 868, 1061
AflII	CTTAAG	2 cut(s)	239, 752
AgeI	ACCGGT	1 cut(s)	293
AgsI	TTSAA	5 cut(s)	175, 280, 602, 681, 968
AhdI	GACNNNNNGTC	1 cut(s)	191
AhlI	ACTAGT	1 cut(s)	538
AluBI	AGCT	7 cut(s)	244, 479, 642, 856, 935, 1033, 1081
AluI	AGCT	7 cut(s)	244, 479, 642, 856, 935, 1033, 1081
Alw21I	GWGCWC	1 cut(s)	1083
Alw26I	GTCTC	1 cut(s)	523
AlwI	GGATC	3 cut(s)	222, 847, 895
ApeKI	GCWGC	2 cut(s)	476, 872
ApoI	RAATTY	2 cut(s)	370, 602
AseI	ATTAAT	1 cut(s)	270
AsiGI	ACCGGT	1 cut(s)	293
Asp700I	GAANNNNTTC	1 cut(s)	170
AspA2I	CCTAGG	1 cut(s)	143
AspS9I	GGNCC	1 cut(s)	329
AsuHPI	GGTGA	2 cut(s)	619, 1076
AvaII	GGWCC	1 cut(s)	329
AvrII	CCTAGG	1 cut(s)	143
BaeI	ACNNNNGTAYC	2 cut(s)	607, 640
BanII	GRGCYC	1 cut(s)	1083
Bbv12I	GWGCWC	1 cut(s)	1083
BbvI	GCAGC	2 cut(s)	488, 884
BccI	CCATC	4 cut(s)	114, 656, 704, 1003
BceAI	ACGGC	1 cut(s)	706
BcgI	CGANNNNNNTGC	2 cut(s)	725, 759
BciVI	GTATCC	1 cut(s)	1032
BclI	TGATCA	1 cut(s)	361
BcoDI	GTCTC	1 cut(s)	523
BcuI	ACTAGT	1 cut(s)	538
BfaI	CTAG	3 cut(s)	144, 539, 1084
BfrI	CTTAAG	2 cut(s)	239, 752
BfuI	GTATCC	1 cut(s)	1032
BisI	GCNGC	3 cut(s)	340, 477, 873
BlnI	CCTAGG	1 cut(s)	143
BlsI	GCNGC	3 cut(s)	341, 478, 874
Bme18I	GGWCC	1 cut(s)	329
BmeRI	GACNNNNNGTC	1 cut(s)	191
BmgT120I	GGNCC	1 cut(s)	329
BmiI	GGNNCC	2 cut(s)	128, 298
BmsI	GCATC	3 cut(s)	319, 781, 1025
Bsa29I	ATCGAT	1 cut(s)	660
BsaBI	GATNNNNATC	1 cut(s)	642
BsaI	GGTCTC	1 cut(s)	523
BsaJI	CCNNGG	2 cut(s)	143, 862
BsaWI	WCCGGW	2 cut(s)	293, 985
BsaXI	ACNNNNNCTCC	2 cut(s)	1004, 1034
Bsc4I	CCNNNNNNNGG	6 cut(s)	144, 338, 399, 653, 868, 1061
Bse118I	RCCGGY	1 cut(s)	293
Bse1I	ACTGG	2 cut(s)	519, 811
Bse8I	GATNNNNATC	1 cut(s)	642
BseCI	ATCGAT	1 cut(s)	660
BseDI	CCNNGG	2 cut(s)	143, 862
BseJI	GATNNNNATC	1 cut(s)	642
BseLI	CCNNNNNNNGG	6 cut(s)	144, 338, 399, 653, 868, 1061
BseMII	CTCAG	1 cut(s)	882
BseNI	ACTGG	2 cut(s)	519, 811
BseRI	GAGGAG	1 cut(s)	139
BseXI	GCAGC	2 cut(s)	488, 884
Bsh1236I	CGCG	1 cut(s)	339
Bsh1285I	CGRYCG	1 cut(s)	879
BshTI	ACCGGT	1 cut(s)	293
BshVI	ATCGAT	1 cut(s)	660
BsiEI	CGRYCG	1 cut(s)	879
BsiHKAI	GWGCWC	1 cut(s)	1083
BsiSI	CCGG	2 cut(s)	294, 986
BslI	CCNNNNNNNGG	6 cut(s)	144, 338, 399, 653, 868, 1061
BsmAI	GTCTC	1 cut(s)	523
BsmI	GAATGC	2 cut(s)	289, 461
Bso31I	GGTCTC	1 cut(s)	523
Bsp1286I	GDGCHC	1 cut(s)	1083
Bsp143I	GATC	6 cut(s)	227, 361, 498, 839, 887, 1026
Bsp19I	CCATGG	1 cut(s)	862
BspACI	CCGC	3 cut(s)	339, 875, 999
BspCNI	CTCAG	1 cut(s)	883
BspDI	ATCGAT	1 cut(s)	660
BspFNI	CGCG	1 cut(s)	339
BspHI	TCATGA	1 cut(s)	364
BspLI	GGNNCC	2 cut(s)	128, 298
BspPI	GGATC	3 cut(s)	222, 847, 895
BspQI	GCTCTTC	1 cut(s)	753
BspTI	CTTAAG	2 cut(s)	239, 752
BspTNI	GGTCTC	1 cut(s)	523
BsrFI	RCCGGY	1 cut(s)	293
BsrI	ACTGG	2 cut(s)	519, 811
BssAI	RCCGGY	1 cut(s)	293
BssECI	CCNNGG	2 cut(s)	143, 862
BssMI	GATC	6 cut(s)	227, 361, 498, 839, 887, 1026
BssT1I	CCWWGG	2 cut(s)	143, 862
Bst4CI	ACNGT	6 cut(s)	351, 387, 472, 525, 620, 1064
Bst6I	CTCTTC	3 cut(s)	87, 161, 753
BstAFI	CTTAAG	2 cut(s)	239, 752
BstAPI	GCANNNNNTGC	1 cut(s)	791
BstC8I	GCNNGC	1 cut(s)	695
BstDEI	CTNAG	2 cut(s)	555, 891
BstDSI	CCRYGG	1 cut(s)	862
BstENI	CCTNNNNNAGG	1 cut(s)	651
BstFNI	CGCG	1 cut(s)	339
BstKTI	GATC	6 cut(s)	230, 364, 501, 842, 890, 1029
BstMAI	GTCTC	1 cut(s)	523
BstMBI	GATC	6 cut(s)	227, 361, 498, 839, 887, 1026
BstMCI	CGRYCG	1 cut(s)	879
BstMWI	GCNNNNNNNGC	3 cut(s)	96, 791, 1013
BstNSI	RCATGY	1 cut(s)	203
BstUI	CGCG	1 cut(s)	339
BstV1I	GCAGC	2 cut(s)	488, 884
BstX2I	RGATCY	2 cut(s)	839, 887
BstYI	RGATCY	2 cut(s)	839, 887
Bsu15I	ATCGAT	1 cut(s)	660
BsuI	GTATCC	1 cut(s)	1032
BsuTUI	ATCGAT	1 cut(s)	660
BtgI	CCRYGG	1 cut(s)	862
BtgZI	GCGATG	1 cut(s)	1022
BtsIMutI	CAGTG	3 cut(s)	420, 521, 630
Cac8I	GCNNGC	1 cut(s)	695
CciI	TCATGA	1 cut(s)	364
Cfr10I	RCCGGY	1 cut(s)	293
Cfr13I	GGNCC	1 cut(s)	329
ClaI	ATCGAT	1 cut(s)	660
CseI	GACGC	1 cut(s)	683
Csp6I	GTAC	1 cut(s)	725
CspAI	ACCGGT	1 cut(s)	293
CviAII	CATG	7 cut(s)	196, 200, 355, 365, 716, 863, 927
CviQI	GTAC	1 cut(s)	725
DdeI	CTNAG	2 cut(s)	555, 891
DpnI	GATC	6 cut(s)	229, 363, 500, 841, 889, 1028
DpnII	GATC	6 cut(s)	227, 361, 498, 839, 887, 1026
DrdI	GACNNNNNNGTC	1 cut(s)	1067
DriI	GACNNNNNGTC	1 cut(s)	191
DseDI	GACNNNNNNGTC	1 cut(s)	1067
Eam1104I	CTCTTC	3 cut(s)	87, 161, 753
Eam1105I	GACNNNNNGTC	1 cut(s)	191
EarI	CTCTTC	3 cut(s)	87, 161, 753
Ecl136II	GAGCTC	1 cut(s)	1081
Eco130I	CCWWGG	2 cut(s)	143, 862
Eco24I	GRGCYC	1 cut(s)	1083
Eco31I	GGTCTC	1 cut(s)	523
Eco47I	GGWCC	1 cut(s)	329
Eco53kI	GAGCTC	1 cut(s)	1081
Eco57I	CTGAAG	1 cut(s)	654
EcoICRI	GAGCTC	1 cut(s)	1081
EcoNI	CCTNNNNNAGG	1 cut(s)	651
EcoRI	GAATTC	1 cut(s)	602
EcoT14I	CCWWGG	2 cut(s)	143, 862
EcoT22I	ATGCAT	2 cut(s)	201, 289
EcoT38I	GRGCYC	1 cut(s)	1083
ErhI	CCWWGG	2 cut(s)	143, 862
FaeI	CATG	7 cut(s)	199, 203, 358, 368, 719, 866, 930
FatI	CATG	7 cut(s)	195, 199, 354, 364, 715, 862, 926
FbaI	TGATCA	1 cut(s)	361
Fnu4HI	GCNGC	3 cut(s)	340, 477, 873
FriOI	GRGCYC	1 cut(s)	1083
Fsp4HI	GCNGC	3 cut(s)	340, 477, 873
FspBI	CTAG	3 cut(s)	144, 539, 1084
GluI	GCNGC	3 cut(s)	340, 477, 873
HapII	CCGG	2 cut(s)	294, 986
HgaI	GACGC	1 cut(s)	683
Hin1II	CATG	7 cut(s)	199, 203, 358, 368, 719, 866, 930
HinfI	GANTC	5 cut(s)	60, 148, 171, 433, 489
HpaII	CCGG	2 cut(s)	294, 986
HphI	GGTGA	2 cut(s)	619, 1076
Hpy166II	GTNNAC	2 cut(s)	205, 834
Hpy188I	TCNGA	6 cut(s)	185, 529, 805, 823, 892, 1026
Hpy188III	TCNNGA	5 cut(s)	280, 365, 430, 502, 610
Hpy8I	GTNNAC	2 cut(s)	205, 834
Hpy99I	CGWCG	4 cut(s)	74, 677, 740, 1042
HpyAV	CCTTC	3 cut(s)	560, 647, 983
HpyCH4III	ACNGT	6 cut(s)	351, 387, 472, 525, 620, 1064
HpyCH4IV	ACGT	1 cut(s)	177
HpyCH4V	TGCA	7 cut(s)	199, 257, 287, 325, 596, 764, 785
HpyF10VI	GCNNNNNNNGC	3 cut(s)	96, 791, 1013
HpyF3I	CTNAG	2 cut(s)	555, 891
HpySE526I	ACGT	1 cut(s)	177
Hsp92II	CATG	7 cut(s)	199, 203, 358, 368, 719, 866, 930
Ksp22I	TGATCA	1 cut(s)	361
Kzo9I	GATC	6 cut(s)	227, 361, 498, 839, 887, 1026
LguI	GCTCTTC	1 cut(s)	753
LmnI	GCTCC	2 cut(s)	126, 932
LpnPI	CCDG	6 cut(s)	307, 500, 780, 786, 824, 999
Lsp1109I	GCAGC	2 cut(s)	488, 884
LweI	GCATC	3 cut(s)	319, 781, 1025
MaeI	CTAG	3 cut(s)	144, 539, 1084
MaeII	ACGT	1 cut(s)	177
MaeIII	GTNAC	3 cut(s)	418, 572, 700
MalI	GATC	6 cut(s)	229, 363, 500, 841, 889, 1028
MboI	GATC	6 cut(s)	227, 361, 498, 839, 887, 1026
MboII	GAAGA	8 cut(s)	16, 104, 178, 232, 625, 647, 731, 740
MfeI	CAATTG	1 cut(s)	786
MflI	RGATCY	2 cut(s)	839, 887
MhlI	GDGCHC	1 cut(s)	1083
MluCI	AATT	5 cut(s)	267, 370, 380, 602, 786
MlyI	GAGTC	1 cut(s)	157
MmeI	TCCRAC	1 cut(s)	324
Mph1103I	ATGCAT	2 cut(s)	201, 289
MroXI	GAANNNNTTC	1 cut(s)	170
MseI	TTAA	4 cut(s)	240, 263, 270, 753
MslI	CAYNNNNRTG	1 cut(s)	720
MspA1I	CMGCKG	2 cut(s)	479, 875
MspCI	CTTAAG	2 cut(s)	239, 752
MspI	CCGG	2 cut(s)	294, 986
MunI	CAATTG	1 cut(s)	786
Mva1269I	GAATGC	2 cut(s)	289, 461
MvnI	CGCG	1 cut(s)	339
MwoI	GCNNNNNNNGC	3 cut(s)	96, 791, 1013
NcoI	CCATGG	1 cut(s)	862
NdeII	GATC	6 cut(s)	227, 361, 498, 839, 887, 1026
NlaIII	CATG	7 cut(s)	199, 203, 358, 368, 719, 866, 930
NlaIV	GGNNCC	2 cut(s)	128, 298
NmuCI	GTSAC	2 cut(s)	418, 700
NsiI	ATGCAT	2 cut(s)	201, 289
NspI	RCATGY	1 cut(s)	203
PagI	TCATGA	1 cut(s)	364
PciSI	GCTCTTC	1 cut(s)	753
PcsI	WCGNNNNNNNCGW	1 cut(s)	69
PctI	GAATGC	2 cut(s)	289, 461
PdmI	GAANNNNTTC	1 cut(s)	170
PfeI	GAWTC	4 cut(s)	60, 171, 433, 489
PflMI	CCANNNNNTGG	1 cut(s)	868
PinAI	ACCGGT	1 cut(s)	293
PkrI	GCNGC	3 cut(s)	341, 478, 874
PleI	GAGTC	1 cut(s)	156
PpsI	GAGTC	1 cut(s)	156
PshBI	ATTAAT	1 cut(s)	270
Psp124BI	GAGCTC	1 cut(s)	1083
Psp1406I	AACGTT	1 cut(s)	177
PspN4I	GGNNCC	2 cut(s)	128, 298
PspPI	GGNCC	1 cut(s)	329
PsrI	GAACNNNNNNTAC	2 cut(s)	215, 247
PsuI	RGATCY	2 cut(s)	839, 887
PvuII	CAGCTG	1 cut(s)	479
RsaI	GTAC	1 cut(s)	726
RsaNI	GTAC	1 cut(s)	725
RseI	CAYNNNNRTG	1 cut(s)	720
SacI	GAGCTC	1 cut(s)	1083
SapI	GCTCTTC	1 cut(s)	753
SaqAI	TTAA	4 cut(s)	240, 263, 270, 753
SatI	GCNGC	3 cut(s)	340, 477, 873
Sau3AI	GATC	6 cut(s)	227, 361, 498, 839, 887, 1026
Sau96I	GGNCC	1 cut(s)	329
SchI	GAGTC	1 cut(s)	157
SduI	GDGCHC	1 cut(s)	1083
SfaNI	GCATC	3 cut(s)	319, 781, 1025
SinI	GGWCC	1 cut(s)	329
SmiMI	CAYNNNNRTG	1 cut(s)	720
SmlI	CTYRAG	2 cut(s)	239, 752
SmoI	CTYRAG	2 cut(s)	239, 752
SpeI	ACTAGT	1 cut(s)	538
Sse9I	AATT	5 cut(s)	267, 370, 380, 602, 786
SsiI	CCGC	3 cut(s)	339, 875, 999
SspMI	CTAG	3 cut(s)	144, 539, 1084
SstI	GAGCTC	1 cut(s)	1083
StyI	CCWWGG	2 cut(s)	143, 862
TaaI	ACNGT	6 cut(s)	351, 387, 472, 525, 620, 1064
TaiI	ACGT	1 cut(s)	180
TaqI	TCGA	2 cut(s)	63, 660
TasI	AATT	5 cut(s)	267, 370, 380, 602, 786
TauI	GCSGC	1 cut(s)	342
TfiI	GAWTC	4 cut(s)	60, 171, 433, 489
Tru1I	TTAA	4 cut(s)	240, 263, 270, 753
Tru9I	TTAA	4 cut(s)	240, 263, 270, 753
TscAI	CASTG	3 cut(s)	427, 528, 637
TseFI	GTSAC	2 cut(s)	418, 700
TseI	GCWGC	2 cut(s)	476, 872
Tsp45I	GTSAC	2 cut(s)	418, 700
TspDTI	ATGAA	8 cut(s)	17, 171, 343, 579, 595, 666, 732, 996
TspGWI	ACGGA	3 cut(s)	127, 382, 591
TspRI	CASTG	3 cut(s)	427, 528, 637
Van91I	CCANNNNNTGG	1 cut(s)	868
Vha464I	CTTAAG	2 cut(s)	239, 752
VpaK11BI	GGWCC	1 cut(s)	329
VspI	ATTAAT	1 cut(s)	270
XagI	CCTNNNNNAGG	1 cut(s)	651
XapI	RAATTY	2 cut(s)	370, 602
XceI	RCATGY	1 cut(s)	203
XcmI	CCANNNNNNNNNTGG	1 cut(s)	53
XmaJI	CCTAGG	1 cut(s)	143
XmnI	GAANNNNTTC	1 cut(s)	170
XspI	CTAG	3 cut(s)	144, 539, 1084
Zsp2I	ATGCAT	2 cut(s)	201, 289

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.