Rorug04G0259400

Belongs to the peptidase C1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0003551
Physical Location & Seq
Forward (+)
43216417 .. 43219092
2676 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0259400.1

Sequence Viewer

Length: 1716 bp
ATGGCGGGTGCGCGTGTTTGCGCCACCTTCCCCAATCTCGCACACCCAATCTTACCCGCCACCACCCCGCCCAAAATTCCGGTATCAGCCTCCGTTTCCGTACCGTCAAAAATCACTCGCCGGAAGAACTATTTGCGCCCCAAAATTCTCAAAACCCTAAACCCTAAACCCGACCCGCAACCCGCACAGCCCGTAGTTCCCGTCGAATCTCCAGTAACCCCATCCCAAGACAACCATGAATTCGAGCATAGCTCCGACCGAGGCGACGTCGTTTTGGGAGGAGCTGAAGTCGAAGAATTAAGAGTGTCTGAGACGACGGCGGAGCTCAGTGGCATTTCTGGTAAATTTTCTGCGAGGACTGTGCTAAAATATGGGGGTTATTTTATTGGGCTTTTTGTGCTACAGACTGTTGTTTCTGTTTTGTTATTGGGAGATAGTGATCCTGATGAAAATAGCAAGTCTAGGAGTTCAGTATTAGGCAAATCCAATTTGAAGACGAGTTCTGGGTCTGAATTGGGCAATGTGGTTCGGTTCGATGATTCCCGATTGGGGGAGAAAATTGAAGAGATTAGGGCAATGGCAAGGAAAGCGCGAAAGATTGAGAAGAATGAGTCCAAGGGAAGTATTGGAAATGAGGATGATGCATTTTCGAAATCTAAGAATAAGATTGGGATTGAAAAGGAGGTTGGGAATCGGTTGGTTAACTTGCAGAAGAGGTTGGGTTCCAAAAGGGAGAAGTTGCCGGGTTCGTTTGTGAATTATCTGGAAATGTTTGGGAAGTTTGAAGATGGGGTGAGGAAGAATAATGAGAATGTGAAGGAGGGGGATGGGGCGTTGATGTTTAAGAAGAAGCTGAAGTTTAAGACTCCTACTGCAGAGGTGAATAGGAATCCGAAGGGTTTTGGAGGTGTCAAAGAGGAAGGTGGTTCTAGTAAGAGTAAGAGAGGTTCAGGTGGTGTAGATGAAATTGGGAGAAATGCAAGTGTTGGTACTGATGGTATGGAATTGCTGGAAGCTGATGCTAAGGAGAAGCCGCGAGAGACTTTGGAGAAAGTAACAGGGAGATTAAATGATAGACAGGAAAGAGAAGCTGGAAATATGGAACCGCAGAATGGTACTGTCCACAGGACTATACAAGGGAGGTCATCTGCTGAAGATGTAGAGTCAATCAAACCAAGAGGCTTCGGGAAGAAAAACCCTGAAAGCTTGAAGAAAGAAATTCAGGGAAGGACAGTAGAATCAGATGGTCCTACTGTGCAGAGTGTGAATAGCGGTTCAACACAGAAAAAAGCCAAAAAACAAGCACTGGCTAACAAAGTCAGTAGTAAGCAGTCAGACAATGAGACTGATCCATGGTGGTTGCATCTTCCTTATGTTCTAGTTATTTTTATGCGTAGAGGTTCTGATGGGCAAGGAGGGCTTTTTAGCATAGAGCTCAATTCTCAGTCCCAAAATGAGAGTGATGCTTCCTATACTGTTGCTTTTGAGGACCGTGGTGATGCCAATAACTTCTGTTTCCTATTGGAGTCTTGTTTTGAAGATCAAGATGATTTCAGGGCCACTGCTGCTCCCTTACCAAATAAAGAACTTCATAAAGCAGTAAAATCAGATGGCATGAAGGTAATTGTTGTGAAGAAGGGGCAGCTTCAGCTGTATGCTGGGCAACCTTTTGCTGAAGTTGAAATGGCTTTGCGCTCTTTAGTTTTACAAAACTGA

Protein Analysis

571

Amino Acids

62.42

Weight (kDa)

9.12

Isoelectric Point (pI)

43.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF3110 PF11360 470 - 549 2.6e-06 Protein of unknown function (DUF3110)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 270
AccII CGCG 3 cut(s) 13, 592, 1036
AciI CCGC 9 cut(s) 5, 57, 68, 176, 183, 320, 1034, 1106, 1272
AclWI GGATC 2 cut(s) 434, 1343
AcsI RAATTY 5 cut(s) 75, 144, 239, 344, 1218
AcuI CTGAAG 5 cut(s) 306, 875, 1173, 1631, 1695
AcyI GRCGYC 1 cut(s) 267
AfaI GTAC 3 cut(s) 102, 991, 1117
AfiI CCNNNNNNNGG 3 cut(s) 549, 550, 1112
AgsI TTSAA 8 cut(s) 493, 563, 677, 785, 1210, 1278, 1538, 1682
AjuI GAANNNNNNNTTGG 2 cut(s) 669, 701
Alw21I GWGCWC 2 cut(s) 327, 1437
Alw26I GTCTC 3 cut(s) 305, 1034, 1337
AlwI GGATC 2 cut(s) 434, 1343
AoxI GGCC 1 cut(s) 1557
ApeKI GCWGC 2 cut(s) 1565, 1642
ApoI RAATTY 5 cut(s) 75, 144, 239, 344, 1218
AspLEI GCGC 5 cut(s) 13, 23, 138, 592, 1695
AspS9I GGNCC 3 cut(s) 1247, 1489, 1557
AsuC2I CCSGG 1 cut(s) 744
AsuHPI GGTGA 3 cut(s) 805, 892, 1508
AsuII TTCGAA 1 cut(s) 650
AvaII GGWCC 2 cut(s) 1247, 1489
BanII GRGCYC 2 cut(s) 327, 1437
BbsI GAAGAC 1 cut(s) 500
Bbv12I GWGCWC 2 cut(s) 327, 1437
BbvI GCAGC 2 cut(s) 1552, 1654
BccI CCATC 7 cut(s) 229, 782, 821, 989, 1238, 1400, 1604
BceAI ACGGC 1 cut(s) 333
BcgI CGANNNNNNTGC 2 cut(s) 343, 377
BcnI CCSGG 1 cut(s) 744
BcoDI GTCTC 3 cut(s) 305, 1034, 1337
BfaI CTAG 3 cut(s) 462, 930, 1379
BfmI CTRYAG 2 cut(s) 401, 873
BisI GCNGC 3 cut(s) 1034, 1566, 1643
BlsI GCNGC 3 cut(s) 1035, 1567, 1644
Bme1390I CCNGG 1 cut(s) 744
Bme18I GGWCC 2 cut(s) 1247, 1489
BmgT120I GGNCC 3 cut(s) 1247, 1489, 1557
BmiI GGNNCC 2 cut(s) 724, 1104
BmrFI CCNGG 1 cut(s) 744
BmsI GCATC 5 cut(s) 631, 1009, 1372, 1453, 1489
BpiI GAAGAC 1 cut(s) 500
BplI GAGNNNNNCTC 2 cut(s) 236, 268
BpmI CTGGAG 1 cut(s) 195
Bpu10I CCTNAGC 1 cut(s) 1023
Bpu14I TTCGAA 1 cut(s) 650
BpuMI CCSGG 1 cut(s) 744
BsaBI GATNNNNATC 1 cut(s) 438
BsaHI GRCGYC 1 cut(s) 267
BsaJI CCNNGG 4 cut(s) 259, 615, 1352, 1492
BsaWI WCCGGW 1 cut(s) 79
BsaXI ACNNNNNCTCC 4 cut(s) 273, 303, 1552, 1582
Bsc4I CCNNNNNNNGG 3 cut(s) 549, 550, 1112
Bse1I ACTGG 2 cut(s) 212, 1311
Bse3DI GCAATG 2 cut(s) 526, 582
Bse8I GATNNNNATC 1 cut(s) 438
BseDI CCNNGG 4 cut(s) 259, 615, 1352, 1492
BseGI GGATG 3 cut(s) 221, 643, 832
BseJI GATNNNNATC 1 cut(s) 438
BseLI CCNNNNNNNGG 3 cut(s) 549, 550, 1112
BseMI GCAATG 2 cut(s) 526, 582
BseMII CTCAG 3 cut(s) 300, 340, 1457
BseNI ACTGG 2 cut(s) 212, 1311
BseRI GAGGAG 1 cut(s) 294
BseXI GCAGC 2 cut(s) 1552, 1654
BseYI CCCAGC 1 cut(s) 1658
BsgI GTGCAG 1 cut(s) 1277
Bsh1236I CGCG 3 cut(s) 13, 592, 1036
Bsh1285I CGRYCG 1 cut(s) 259
BshFI GGCC 1 cut(s) 1559
BsiEI CGRYCG 1 cut(s) 259
BsiHKAI GWGCWC 2 cut(s) 327, 1437
BsiSI CCGG 3 cut(s) 80, 121, 743
BslFI GGGAC 1 cut(s) 1432
BslI CCNNNNNNNGG 3 cut(s) 549, 550, 1112
BsmAI GTCTC 3 cut(s) 305, 1034, 1337
BsmBI CGTCTC 1 cut(s) 305
BsmFI GGGAC 1 cut(s) 1432
BsnI GGCC 1 cut(s) 1559
Bsp119I TTCGAA 1 cut(s) 650
Bsp1286I GDGCHC 2 cut(s) 327, 1437
Bsp143I GATC 3 cut(s) 439, 1348, 1540
Bsp19I CCATGG 1 cut(s) 1352
BspACI CCGC 9 cut(s) 5, 57, 68, 176, 183, 320, 1034, 1106, 1272
BspANI GGCC 1 cut(s) 1559
BspCNI CTCAG 3 cut(s) 301, 339, 1456
BspFNI CGCG 3 cut(s) 13, 592, 1036
BspLI GGNNCC 2 cut(s) 724, 1104
BspMAI CTGCAG 1 cut(s) 877
BspPI GGATC 2 cut(s) 434, 1343
BspT104I TTCGAA 1 cut(s) 650
BsrDI GCAATG 2 cut(s) 526, 582
BsrI ACTGG 2 cut(s) 212, 1311
BssECI CCNNGG 4 cut(s) 259, 615, 1352, 1492
BssMI GATC 3 cut(s) 439, 1348, 1540
BssNI GRCGYC 1 cut(s) 267
BssT1I CCWWGG 2 cut(s) 615, 1352
Bst4CI ACNGT 8 cut(s) 105, 361, 409, 1120, 1234, 1255, 1477, 1493
Bst6I CTCTTC 2 cut(s) 558, 707
BstACI GRCGYC 1 cut(s) 267
BstBI TTCGAA 1 cut(s) 650
BstDEI CTNAG 5 cut(s) 309, 326, 657, 1023, 1443
BstDSI CCRYGG 2 cut(s) 1352, 1492
BstF5I GGATG 3 cut(s) 221, 643, 832
BstFNI CGCG 3 cut(s) 13, 592, 1036
BstHHI GCGC 5 cut(s) 13, 23, 138, 592, 1695
BstKTI GATC 3 cut(s) 442, 1351, 1543
BstMAI GTCTC 3 cut(s) 305, 1034, 1337
BstMBI GATC 3 cut(s) 439, 1348, 1540
BstMCI CGRYCG 1 cut(s) 259
BstMWI GCNNNNNNNGC 5 cut(s) 397, 587, 1417, 1565, 1648
BstSCI CCNGG 1 cut(s) 742
BstSFI CTRYAG 2 cut(s) 401, 873
BstUI CGCG 3 cut(s) 13, 592, 1036
BstV1I GCAGC 2 cut(s) 1552, 1654
BstV2I GAAGAC 1 cut(s) 500
BsuRI GGCC 1 cut(s) 1559
BtgI CCRYGG 2 cut(s) 1352, 1492
BtsCI GGATG 3 cut(s) 221, 643, 832
BtsI GCAGTG 1 cut(s) 1560
BtsIMutI CAGTG 3 cut(s) 334, 1304, 1560
CfoI GCGC 5 cut(s) 13, 23, 138, 592, 1695
Cfr13I GGNCC 3 cut(s) 1247, 1489, 1557
Csp6I GTAC 3 cut(s) 101, 990, 1116
CviAII CATG 3 cut(s) 236, 1353, 1615
CviQI GTAC 3 cut(s) 101, 990, 1116
DdeI CTNAG 5 cut(s) 309, 326, 657, 1023, 1443
DpnI GATC 3 cut(s) 441, 1350, 1542
DpnII GATC 3 cut(s) 439, 1348, 1540
Eam1104I CTCTTC 2 cut(s) 558, 707
EarI CTCTTC 2 cut(s) 558, 707
EciI GGCGGA 1 cut(s) 335
Ecl136II GAGCTC 2 cut(s) 325, 1435
Eco130I CCWWGG 2 cut(s) 615, 1352
Eco24I GRGCYC 2 cut(s) 327, 1437
Eco47I GGWCC 2 cut(s) 1247, 1489
Eco53kI GAGCTC 2 cut(s) 325, 1435
Eco57I CTGAAG 5 cut(s) 306, 875, 1173, 1631, 1695
EcoICRI GAGCTC 2 cut(s) 325, 1435
EcoRI GAATTC 1 cut(s) 239
EcoT14I CCWWGG 2 cut(s) 615, 1352
EcoT22I ATGCAT 1 cut(s) 646
EcoT38I GRGCYC 2 cut(s) 327, 1437
ErhI CCWWGG 2 cut(s) 615, 1352
Esp3I CGTCTC 1 cut(s) 305
FaeI CATG 3 cut(s) 239, 1356, 1618
FalI AAGNNNNNCTT 2 cut(s) 1404, 1436
FaqI GGGAC 1 cut(s) 1432
FatI CATG 3 cut(s) 235, 1352, 1614
FauI CCCGC 4 cut(s) 64, 75, 183, 190
Fnu4HI GCNGC 3 cut(s) 1034, 1566, 1643
FokI GGATG 3 cut(s) 208, 650, 839
FriOI GRGCYC 2 cut(s) 327, 1437
Fsp4HI GCNGC 3 cut(s) 1034, 1566, 1643
FspBI CTAG 3 cut(s) 462, 930, 1379
GlaI GCGC 5 cut(s) 12, 22, 137, 591, 1694
GluI GCNGC 3 cut(s) 1034, 1566, 1643
GsaI CCCAGC 1 cut(s) 1662
GsuI CTGGAG 1 cut(s) 195
HaeIII GGCC 1 cut(s) 1559
HapII CCGG 3 cut(s) 80, 121, 743
HhaI GCGC 5 cut(s) 13, 23, 138, 592, 1695
Hin1I GRCGYC 1 cut(s) 267
Hin1II CATG 3 cut(s) 239, 1356, 1618
Hin6I GCGC 5 cut(s) 11, 21, 136, 590, 1693
HinP1I GCGC 5 cut(s) 11, 21, 136, 590, 1693
HincII GTYRAC 1 cut(s) 703
HindII GTYRAC 1 cut(s) 703
HindIII AAGCTT 1 cut(s) 1204
HinfI GANTC 9 cut(s) 206, 539, 611, 691, 865, 889, 1163, 1238, 1526
HpaI GTTAAC 1 cut(s) 703
HpaII CCGG 3 cut(s) 80, 121, 743
HphI GGTGA 3 cut(s) 805, 892, 1508
Hpy166II GTNNAC 2 cut(s) 703, 1123
Hpy188I TCNGA 8 cut(s) 256, 310, 511, 894, 1243, 1336, 1405, 1609
Hpy188III TCNNGA 5 cut(s) 443, 543, 764, 1186, 1544
Hpy8I GTNNAC 2 cut(s) 703, 1123
Hpy99I CGWCG 4 cut(s) 206, 269, 272, 319
HpyAV CCTTC 7 cut(s) 37, 811, 889, 914, 1221, 1612, 1630
HpyCH4III ACNGT 8 cut(s) 105, 361, 409, 1120, 1234, 1255, 1477, 1493
HpyCH4IV ACGT 1 cut(s) 267
HpyCH4V TGCA 6 cut(s) 644, 709, 875, 980, 1258, 1363
HpyF10VI GCNNNNNNNGC 5 cut(s) 397, 587, 1417, 1565, 1648
HpyF3I CTNAG 5 cut(s) 309, 326, 657, 1023, 1443
HpySE526I ACGT 1 cut(s) 267
Hsp92I GRCGYC 1 cut(s) 267
Hsp92II CATG 3 cut(s) 239, 1356, 1618
HspAI GCGC 5 cut(s) 11, 21, 136, 590, 1693
KspAI GTTAAC 1 cut(s) 703
Kzo9I GATC 3 cut(s) 439, 1348, 1540
LmnI GCTCC 4 cut(s) 257, 281, 322, 1573
Lsp1109I GCAGC 2 cut(s) 1552, 1654
LweI GCATC 5 cut(s) 631, 1009, 1372, 1453, 1489
MaeI CTAG 3 cut(s) 462, 930, 1379
MaeII ACGT 1 cut(s) 267
MaeIII GTNAC 2 cut(s) 214, 1054
MalI GATC 3 cut(s) 441, 1350, 1542
MboI GATC 3 cut(s) 439, 1348, 1540
MhlI GDGCHC 2 cut(s) 327, 1437
MlyI GAGTC 4 cut(s) 620, 859, 1172, 1535
MmeI TCCRAC 1 cut(s) 279
Mph1103I ATGCAT 1 cut(s) 646
MseI TTAA 5 cut(s) 299, 702, 843, 861, 1067
MspA1I CMGCKG 1 cut(s) 1651
MspI CCGG 3 cut(s) 80, 121, 743
MspR9I CCNGG 1 cut(s) 744
MvnI CGCG 3 cut(s) 13, 592, 1036
MwoI GCNNNNNNNGC 5 cut(s) 397, 587, 1417, 1565, 1648
NciI CCSGG 1 cut(s) 744
NcoI CCATGG 1 cut(s) 1352
NdeII GATC 3 cut(s) 439, 1348, 1540
NlaIII CATG 3 cut(s) 239, 1356, 1618
NlaIV GGNNCC 2 cut(s) 724, 1104
NsiI ATGCAT 1 cut(s) 646
NspV TTCGAA 1 cut(s) 650
PfeI GAWTC 5 cut(s) 206, 539, 691, 889, 1238
PkrI GCNGC 3 cut(s) 1035, 1567, 1644
PleI GAGTC 4 cut(s) 619, 859, 1171, 1534
PpsI GAGTC 4 cut(s) 619, 859, 1171, 1534
Psp124BI GAGCTC 2 cut(s) 327, 1437
PspFI CCCAGC 1 cut(s) 1658
PspN4I GGNNCC 2 cut(s) 724, 1104
PspPI GGNCC 3 cut(s) 1247, 1489, 1557
PsrI GAACNNNNNNTAC 2 cut(s) 931, 963
PstI CTGCAG 1 cut(s) 877
PvuII CAGCTG 1 cut(s) 1651
RsaI GTAC 3 cut(s) 102, 991, 1117
RsaNI GTAC 3 cut(s) 101, 990, 1116
SacI GAGCTC 2 cut(s) 327, 1437
SaqAI TTAA 5 cut(s) 299, 702, 843, 861, 1067
SatI GCNGC 3 cut(s) 1034, 1566, 1643
Sau3AI GATC 3 cut(s) 439, 1348, 1540
Sau96I GGNCC 3 cut(s) 1247, 1489, 1557
SchI GAGTC 4 cut(s) 620, 859, 1172, 1535
ScrFI CCNGG 1 cut(s) 744
SduI GDGCHC 2 cut(s) 327, 1437
SfaNI GCATC 5 cut(s) 631, 1009, 1372, 1453, 1489
SfcI CTRYAG 2 cut(s) 401, 873
SfuI TTCGAA 1 cut(s) 650
SinI GGWCC 2 cut(s) 1247, 1489
SsiI CCGC 9 cut(s) 5, 57, 68, 176, 183, 320, 1034, 1106, 1272
SspMI CTAG 3 cut(s) 462, 930, 1379
SstI GAGCTC 2 cut(s) 327, 1437
StyD4I CCNGG 1 cut(s) 742
StyI CCWWGG 2 cut(s) 615, 1352
TaaI ACNGT 8 cut(s) 105, 361, 409, 1120, 1234, 1255, 1477, 1493
TaiI ACGT 1 cut(s) 270
TaqI TCGA 5 cut(s) 204, 243, 291, 534, 650
TaqII GACCGA 1 cut(s) 273
TauI GCSGC 1 cut(s) 1036
TfiI GAWTC 5 cut(s) 206, 539, 691, 889, 1238
Tru1I TTAA 5 cut(s) 299, 702, 843, 861, 1067
Tru9I TTAA 5 cut(s) 299, 702, 843, 861, 1067
TscAI CASTG 3 cut(s) 334, 1311, 1567
TseI GCWGC 2 cut(s) 1565, 1642
TspDTI ATGAA 5 cut(s) 252, 462, 978, 1580, 1631
TspGWI ACGGA 2 cut(s) 82, 88
TspRI CASTG 3 cut(s) 334, 1311, 1567
VpaK11BI GGWCC 2 cut(s) 1247, 1489
XapI RAATTY 5 cut(s) 75, 144, 239, 344, 1218
XspI CTAG 3 cut(s) 462, 930, 1379
ZraI GACGTC 1 cut(s) 268
Zsp2I ATGCAT 1 cut(s) 646
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.