POT family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AasI | GACNNNNNNGTC | 1 cut(s) | 932 |
| AccB7I | CCANNNNNTGG | 1 cut(s) | 1090 |
| AccI | GTMKAC | 1 cut(s) | 20 |
| AccII | CGCG | 3 cut(s) | 434, 659, 671 |
| AciI | CCGC | 7 cut(s) | 94, 462, 671, 761, 896, 1187, 1376 |
| AclWI | GGATC | 2 cut(s) | 749, 1342 |
| AcsI | RAATTY | 1 cut(s) | 403 |
| AcuI | CTGAAG | 2 cut(s) | 336, 695 |
| AfaI | GTAC | 3 cut(s) | 1037, 1149, 1658 |
| AfiI | CCNNNNNNNGG | 3 cut(s) | 81, 251, 1090 |
| AflII | CTTAAG | 1 cut(s) | 977 |
| AflIII | ACRYGT | 1 cut(s) | 230 |
| AgsI | TTSAA | 7 cut(s) | 550, 815, 971, 1106, 1235, 1591, 1671 |
| AjnI | CCWGG | 3 cut(s) | 358, 370, 718 |
| AloI | GAACNNNNNNTCC | 2 cut(s) | 937, 969 |
| AluBI | AGCT | 9 cut(s) | 89, 266, 350, 494, 581, 1118, 1231, 1426, 1694 |
| AluI | AGCT | 9 cut(s) | 89, 266, 350, 494, 581, 1118, 1231, 1426, 1694 |
| Alw21I | GWGCWC | 5 cut(s) | 583, 864, 1052, 1520, 1708 |
| Alw26I | GTCTC | 6 cut(s) | 325, 709, 807, 940, 1264, 1472 |
| Alw44I | GTGCAC | 1 cut(s) | 860 |
| AlwI | GGATC | 2 cut(s) | 749, 1342 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 359 |
| AoxI | GGCC | 3 cut(s) | 129, 1008, 1597 |
| ApaLI | GTGCAC | 1 cut(s) | 860 |
| ApeKI | GCWGC | 3 cut(s) | 200, 590, 893 |
| ApoI | RAATTY | 1 cut(s) | 403 |
| AseI | ATTAAT | 1 cut(s) | 570 |
| AspLEI | GCGC | 1 cut(s) | 436 |
| AspS9I | GGNCC | 2 cut(s) | 1008, 1081 |
| AsuC2I | CCSGG | 1 cut(s) | 1569 |
| AsuHPI | GGTGA | 1 cut(s) | 1402 |
| AvaII | GGWCC | 1 cut(s) | 1081 |
| BaeGI | GKGCMC | 2 cut(s) | 149, 864 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 1364, 1397 |
| BanII | GRGCYC | 1 cut(s) | 583 |
| BbsI | GAAGAC | 1 cut(s) | 1015 |
| Bbv12I | GWGCWC | 5 cut(s) | 583, 864, 1052, 1520, 1708 |
| BbvI | GCAGC | 3 cut(s) | 187, 577, 905 |
| BccI | CCATC | 6 cut(s) | 23, 627, 655, 1120, 1271, 1608 |
| BceAI | ACGGC | 4 cut(s) | 159, 213, 457, 681 |
| BciT130I | CCWGG | 3 cut(s) | 360, 372, 720 |
| BciVI | GTATCC | 2 cut(s) | 603, 1569 |
| BclI | TGATCA | 3 cut(s) | 496, 1275, 1441 |
| BcnI | CCSGG | 1 cut(s) | 1569 |
| BcoDI | GTCTC | 6 cut(s) | 325, 709, 807, 940, 1264, 1472 |
| BfaI | CTAG | 2 cut(s) | 408, 738 |
| BfmI | CTRYAG | 2 cut(s) | 819, 1281 |
| BfrI | CTTAAG | 1 cut(s) | 977 |
| BfuI | GTATCC | 2 cut(s) | 603, 1569 |
| BisI | GCNGC | 5 cut(s) | 94, 201, 591, 762, 894 |
| BlsI | GCNGC | 5 cut(s) | 95, 202, 592, 763, 895 |
| Bme1390I | CCNGG | 4 cut(s) | 360, 372, 720, 1569 |
| Bme18I | GGWCC | 1 cut(s) | 1081 |
| BmgT120I | GGNCC | 2 cut(s) | 1008, 1081 |
| BmiI | GGNNCC | 3 cut(s) | 256, 789, 1449 |
| BmrFI | CCNGG | 4 cut(s) | 360, 372, 720, 1569 |
| BmsI | GCATC | 3 cut(s) | 259, 927, 1444 |
| BoxI | GACNNNNGTC | 1 cut(s) | 1163 |
| BpiI | GAAGAC | 1 cut(s) | 1015 |
| BpmI | CTGGAG | 1 cut(s) | 741 |
| BpuEI | CTTGAG | 3 cut(s) | 188, 948, 1448 |
| BpuMI | CCSGG | 1 cut(s) | 1569 |
| BsaBI | GATNNNNATC | 1 cut(s) | 665 |
| BsaI | GGTCTC | 2 cut(s) | 709, 1264 |
| BsaJI | CCNNGG | 4 cut(s) | 277, 941, 1491, 1594 |
| BsaWI | WCCGGW | 3 cut(s) | 74, 323, 1579 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 937, 967 |
| Bsc4I | CCNNNNNNNGG | 3 cut(s) | 81, 251, 1090 |
| Bse1I | ACTGG | 4 cut(s) | 463, 557, 1001, 1174 |
| Bse3DI | GCAATG | 4 cut(s) | 196, 388, 585, 1216 |
| Bse8I | GATNNNNATC | 1 cut(s) | 665 |
| BseBI | CCWGG | 3 cut(s) | 360, 372, 720 |
| BseDI | CCNNGG | 4 cut(s) | 277, 941, 1491, 1594 |
| BseGI | GGATG | 2 cut(s) | 397, 638 |
| BseJI | GATNNNNATC | 1 cut(s) | 665 |
| BseLI | CCNNNNNNNGG | 3 cut(s) | 81, 251, 1090 |
| BseMI | GCAATG | 4 cut(s) | 196, 388, 585, 1216 |
| BseMII | CTCAG | 2 cut(s) | 1241, 1648 |
| BseNI | ACTGG | 4 cut(s) | 463, 557, 1001, 1174 |
| BseSI | GKGCMC | 2 cut(s) | 149, 864 |
| BseXI | GCAGC | 3 cut(s) | 187, 577, 905 |
| BsgI | GTGCAG | 1 cut(s) | 1419 |
| Bsh1236I | CGCG | 3 cut(s) | 434, 659, 671 |
| BshFI | GGCC | 3 cut(s) | 131, 1010, 1599 |
| BsiHKAI | GWGCWC | 5 cut(s) | 583, 864, 1052, 1520, 1708 |
| BsiSI | CCGG | 5 cut(s) | 75, 324, 850, 1568, 1580 |
| BslI | CCNNNNNNNGG | 3 cut(s) | 81, 251, 1090 |
| BsmAI | GTCTC | 6 cut(s) | 325, 709, 807, 940, 1264, 1472 |
| BsnI | GGCC | 3 cut(s) | 131, 1010, 1599 |
| Bso31I | GGTCTC | 2 cut(s) | 709, 1264 |
| Bsp1286I | GDGCHC | 6 cut(s) | 149, 583, 864, 1052, 1520, 1708 |
| Bsp1407I | TGTACA | 1 cut(s) | 1035 |
| Bsp143I | GATC | 6 cut(s) | 496, 528, 741, 1275, 1347, 1441 |
| Bsp19I | CCATGG | 1 cut(s) | 941 |
| Bsp68I | TCGCGA | 1 cut(s) | 659 |
| BspACI | CCGC | 7 cut(s) | 94, 462, 671, 761, 896, 1187, 1376 |
| BspANI | GGCC | 3 cut(s) | 131, 1010, 1599 |
| BspCNI | CTCAG | 2 cut(s) | 1240, 1647 |
| BspFNI | CGCG | 3 cut(s) | 434, 659, 671 |
| BspLI | GGNNCC | 3 cut(s) | 256, 789, 1449 |
| BspMAI | CTGCAG | 2 cut(s) | 823, 1285 |
| BspPI | GGATC | 2 cut(s) | 749, 1342 |
| BspTI | CTTAAG | 1 cut(s) | 977 |
| BspTNI | GGTCTC | 2 cut(s) | 709, 1264 |
| BsrDI | GCAATG | 4 cut(s) | 196, 388, 585, 1216 |
| BsrGI | TGTACA | 1 cut(s) | 1035 |
| BsrI | ACTGG | 4 cut(s) | 463, 557, 1001, 1174 |
| BssECI | CCNNGG | 4 cut(s) | 277, 941, 1491, 1594 |
| BssMI | GATC | 6 cut(s) | 496, 528, 741, 1275, 1347, 1441 |
| BssNAI | GTATAC | 1 cut(s) | 21 |
| BssT1I | CCWWGG | 2 cut(s) | 277, 941 |
| Bst1107I | GTATAC | 1 cut(s) | 21 |
| Bst2UI | CCWGG | 3 cut(s) | 360, 372, 720 |
| Bst4CI | ACNGT | 9 cut(s) | 37, 598, 655, 1033, 1141, 1495, 1522, 1534, 1562 |
| Bst6I | CTCTTC | 1 cut(s) | 1157 |
| BstAFI | CTTAAG | 1 cut(s) | 977 |
| BstAUI | TGTACA | 1 cut(s) | 1035 |
| BstC8I | GCNNGC | 2 cut(s) | 91, 1191 |
| BstDEI | CTNAG | 3 cut(s) | 1227, 1634, 1651 |
| BstDSI | CCRYGG | 2 cut(s) | 941, 1491 |
| BstF5I | GGATG | 2 cut(s) | 397, 638 |
| BstFNI | CGCG | 3 cut(s) | 434, 659, 671 |
| BstHHI | GCGC | 1 cut(s) | 436 |
| BstKTI | GATC | 6 cut(s) | 499, 531, 744, 1278, 1350, 1444 |
| BstMAI | GTCTC | 6 cut(s) | 325, 709, 807, 940, 1264, 1472 |
| BstMBI | GATC | 6 cut(s) | 496, 528, 741, 1275, 1347, 1441 |
| BstMWI | GCNNNNNNNGC | 6 cut(s) | 200, 263, 587, 1047, 1375, 1454 |
| BstNI | CCWGG | 3 cut(s) | 360, 372, 720 |
| BstNSI | RCATGY | 1 cut(s) | 234 |
| BstPAI | GACNNNNGTC | 1 cut(s) | 1163 |
| BstSCI | CCNGG | 4 cut(s) | 358, 370, 718, 1567 |
| BstSFI | CTRYAG | 2 cut(s) | 819, 1281 |
| BstSLI | GKGCMC | 2 cut(s) | 149, 864 |
| BstUI | CGCG | 3 cut(s) | 434, 659, 671 |
| BstV1I | GCAGC | 3 cut(s) | 187, 577, 905 |
| BstV2I | GAAGAC | 1 cut(s) | 1015 |
| BstX2I | RGATCY | 2 cut(s) | 741, 1347 |
| BstYI | RGATCY | 2 cut(s) | 741, 1347 |
| BstZ17I | GTATAC | 1 cut(s) | 21 |
| BsuI | GTATCC | 2 cut(s) | 603, 1569 |
| BsuRI | GGCC | 3 cut(s) | 131, 1010, 1599 |
| BtgI | CCRYGG | 2 cut(s) | 941, 1491 |
| BtgZI | GCGATG | 1 cut(s) | 674 |
| BtsCI | GGATG | 2 cut(s) | 397, 638 |
| BtsI | GCAGTG | 1 cut(s) | 1278 |
| BtsIMutI | CAGTG | 4 cut(s) | 1038, 1137, 1150, 1278 |
| BtuMI | TCGCGA | 1 cut(s) | 659 |
| Cac8I | GCNNGC | 2 cut(s) | 91, 1191 |
| CaiI | CAGNNNCTG | 1 cut(s) | 359 |
| CfoI | GCGC | 1 cut(s) | 436 |
| Cfr13I | GGNCC | 2 cut(s) | 1008, 1081 |
| CsiI | ACCWGGT | 1 cut(s) | 370 |
| Csp6I | GTAC | 3 cut(s) | 1036, 1148, 1657 |
| CviAII | CATG | 6 cut(s) | 142, 231, 942, 1090, 1273, 1459 |
| CviQI | GTAC | 3 cut(s) | 1036, 1148, 1657 |
| DdeI | CTNAG | 3 cut(s) | 1227, 1634, 1651 |
| DpnI | GATC | 6 cut(s) | 498, 530, 743, 1277, 1349, 1443 |
| DpnII | GATC | 6 cut(s) | 496, 528, 741, 1275, 1347, 1441 |
| DrdI | GACNNNNNNGTC | 1 cut(s) | 932 |
| DseDI | GACNNNNNNGTC | 1 cut(s) | 932 |
| Eam1104I | CTCTTC | 1 cut(s) | 1157 |
| EarI | CTCTTC | 1 cut(s) | 1157 |
| Ecl136II | GAGCTC | 1 cut(s) | 581 |
| Eco130I | CCWWGG | 2 cut(s) | 277, 941 |
| Eco24I | GRGCYC | 1 cut(s) | 583 |
| Eco31I | GGTCTC | 2 cut(s) | 709, 1264 |
| Eco47I | GGWCC | 1 cut(s) | 1081 |
| Eco53kI | GAGCTC | 1 cut(s) | 581 |
| Eco57I | CTGAAG | 2 cut(s) | 336, 695 |
| EcoICRI | GAGCTC | 1 cut(s) | 581 |
| EcoRII | CCWGG | 3 cut(s) | 358, 370, 718 |
| EcoT14I | CCWWGG | 2 cut(s) | 277, 941 |
| EcoT22I | ATGCAT | 1 cut(s) | 1333 |
| EcoT38I | GRGCYC | 1 cut(s) | 583 |
| ErhI | CCWWGG | 2 cut(s) | 277, 941 |
| FaeI | CATG | 6 cut(s) | 145, 234, 945, 1093, 1276, 1462 |
| FatI | CATG | 6 cut(s) | 141, 230, 941, 1089, 1272, 1458 |
| FauI | CCCGC | 1 cut(s) | 1194 |
| FauNDI | CATATG | 2 cut(s) | 1333, 1353 |
| FbaI | TGATCA | 3 cut(s) | 496, 1275, 1441 |
| FblI | GTMKAC | 1 cut(s) | 20 |
| Fnu4HI | GCNGC | 5 cut(s) | 94, 201, 591, 762, 894 |
| FokI | GGATG | 2 cut(s) | 384, 645 |
| FriOI | GRGCYC | 1 cut(s) | 583 |
| Fsp4HI | GCNGC | 5 cut(s) | 94, 201, 591, 762, 894 |
| FspBI | CTAG | 2 cut(s) | 408, 738 |
| GlaI | GCGC | 1 cut(s) | 435 |
| GluI | GCNGC | 5 cut(s) | 94, 201, 591, 762, 894 |
| GsuI | CTGGAG | 1 cut(s) | 741 |
| HaeIII | GGCC | 3 cut(s) | 131, 1010, 1599 |
| HapII | CCGG | 5 cut(s) | 75, 324, 850, 1568, 1580 |
| HhaI | GCGC | 1 cut(s) | 436 |
| Hin1II | CATG | 6 cut(s) | 145, 234, 945, 1093, 1276, 1462 |
| Hin6I | GCGC | 1 cut(s) | 434 |
| HinP1I | GCGC | 1 cut(s) | 434 |
| HincII | GTYRAC | 1 cut(s) | 1488 |
| HindII | GTYRAC | 1 cut(s) | 1488 |
| HindIII | AAGCTT | 1 cut(s) | 87 |
| HinfI | GANTC | 6 cut(s) | 181, 331, 727, 904, 1159, 1287 |
| HpaII | CCGG | 5 cut(s) | 75, 324, 850, 1568, 1580 |
| HphI | GGTGA | 1 cut(s) | 1402 |
| Hpy166II | GTNNAC | 9 cut(s) | 21, 731, 862, 1036, 1148, 1203, 1488, 1565, 1657 |
| Hpy188I | TCNGA | 7 cut(s) | 107, 180, 414, 692, 714, 771, 913 |
| Hpy8I | GTNNAC | 9 cut(s) | 21, 731, 862, 1036, 1148, 1203, 1488, 1565, 1657 |
| HpyCH4III | ACNGT | 9 cut(s) | 37, 598, 655, 1033, 1141, 1495, 1522, 1534, 1562 |
| HpyCH4IV | ACGT | 2 cut(s) | 210, 1237 |
| HpyF10VI | GCNNNNNNNGC | 6 cut(s) | 200, 263, 587, 1047, 1375, 1454 |
| HpyF3I | CTNAG | 3 cut(s) | 1227, 1634, 1651 |
| HpySE526I | ACGT | 2 cut(s) | 210, 1237 |
| Hsp92II | CATG | 6 cut(s) | 145, 234, 945, 1093, 1276, 1462 |
| HspAI | GCGC | 1 cut(s) | 434 |
| Ksp22I | TGATCA | 3 cut(s) | 496, 1275, 1441 |
| Kzo9I | GATC | 6 cut(s) | 496, 528, 741, 1275, 1347, 1441 |
| LmnI | GCTCC | 4 cut(s) | 254, 491, 578, 1453 |
| Lsp1109I | GCAGC | 3 cut(s) | 187, 577, 905 |
| LweI | GCATC | 3 cut(s) | 259, 927, 1444 |
| MabI | ACCWGGT | 1 cut(s) | 370 |
| MaeI | CTAG | 2 cut(s) | 408, 738 |
| MaeII | ACGT | 2 cut(s) | 210, 1237 |
| MaeIII | GTNAC | 6 cut(s) | 211, 374, 833, 958, 1062, 1528 |
| MalI | GATC | 6 cut(s) | 498, 530, 743, 1277, 1349, 1443 |
| MboI | GATC | 6 cut(s) | 496, 528, 741, 1275, 1347, 1441 |
| MflI | RGATCY | 2 cut(s) | 741, 1347 |
| MhlI | GDGCHC | 6 cut(s) | 149, 583, 864, 1052, 1520, 1708 |
| MluCI | AATT | 4 cut(s) | 79, 160, 217, 403 |
| MlyI | GAGTC | 5 cut(s) | 325, 736, 913, 1153, 1296 |
| MmeI | TCCRAC | 3 cut(s) | 556, 674, 1553 |
| MnlI | CCTC | 7 cut(s) | 716, 787, 1211, 1288, 1589, 1652, 1670 |
| Mph1103I | ATGCAT | 1 cut(s) | 1333 |
| MseI | TTAA | 5 cut(s) | 366, 570, 783, 875, 978 |
| MspA1I | CMGCKG | 2 cut(s) | 896, 1189 |
| MspCI | CTTAAG | 1 cut(s) | 977 |
| MspI | CCGG | 5 cut(s) | 75, 324, 850, 1568, 1580 |
| MspR9I | CCNGG | 4 cut(s) | 360, 372, 720, 1569 |
| MvaI | CCWGG | 3 cut(s) | 360, 372, 720 |
| MvnI | CGCG | 3 cut(s) | 434, 659, 671 |
| MwoI | GCNNNNNNNGC | 6 cut(s) | 200, 263, 587, 1047, 1375, 1454 |
| NciI | CCSGG | 1 cut(s) | 1569 |
| NcoI | CCATGG | 1 cut(s) | 941 |
| NdeI | CATATG | 2 cut(s) | 1333, 1353 |
| NdeII | GATC | 6 cut(s) | 496, 528, 741, 1275, 1347, 1441 |
| NlaIII | CATG | 6 cut(s) | 145, 234, 945, 1093, 1276, 1462 |
| NlaIV | GGNNCC | 3 cut(s) | 256, 789, 1449 |
| NmuCI | GTSAC | 2 cut(s) | 211, 1528 |
| NruI | TCGCGA | 1 cut(s) | 659 |
| NsiI | ATGCAT | 1 cut(s) | 1333 |
| NspI | RCATGY | 1 cut(s) | 234 |
| PciI | ACATGT | 1 cut(s) | 230 |
| PcsI | WCGNNNNNNNCGW | 1 cut(s) | 1213 |
| PfeI | GAWTC | 1 cut(s) | 181 |
| PflFI | GACNNNGTC | 1 cut(s) | 1139 |
| PflMI | CCANNNNNTGG | 1 cut(s) | 1090 |
| PfoI | TCCNGGA | 1 cut(s) | 358 |
| PkrI | GCNGC | 5 cut(s) | 95, 202, 592, 763, 895 |
| PleI | GAGTC | 5 cut(s) | 325, 735, 912, 1153, 1295 |
| PpsI | GAGTC | 5 cut(s) | 325, 735, 912, 1153, 1295 |
| PscI | ACATGT | 1 cut(s) | 230 |
| PshAI | GACNNNNGTC | 1 cut(s) | 1163 |
| PshBI | ATTAAT | 1 cut(s) | 570 |
| Psp124BI | GAGCTC | 1 cut(s) | 583 |
| Psp6I | CCWGG | 3 cut(s) | 358, 370, 718 |
| PspGI | CCWGG | 3 cut(s) | 358, 370, 718 |
| PspN4I | GGNNCC | 3 cut(s) | 256, 789, 1449 |
| PspPI | GGNCC | 2 cut(s) | 1008, 1081 |
| PstI | CTGCAG | 2 cut(s) | 823, 1285 |
| PstNI | CAGNNNCTG | 1 cut(s) | 359 |
| PsuI | RGATCY | 2 cut(s) | 741, 1347 |
| PsyI | GACNNNGTC | 1 cut(s) | 1139 |
| RruI | TCGCGA | 1 cut(s) | 659 |
| RsaI | GTAC | 3 cut(s) | 1037, 1149, 1658 |
| RsaNI | GTAC | 3 cut(s) | 1036, 1148, 1657 |
| SacI | GAGCTC | 1 cut(s) | 583 |
| SaqAI | TTAA | 5 cut(s) | 366, 570, 783, 875, 978 |
| SatI | GCNGC | 5 cut(s) | 94, 201, 591, 762, 894 |
| Sau3AI | GATC | 6 cut(s) | 496, 528, 741, 1275, 1347, 1441 |
| Sau96I | GGNCC | 2 cut(s) | 1008, 1081 |
| SchI | GAGTC | 5 cut(s) | 325, 736, 913, 1153, 1296 |
| ScrFI | CCNGG | 4 cut(s) | 360, 372, 720, 1569 |
| SduI | GDGCHC | 6 cut(s) | 149, 583, 864, 1052, 1520, 1708 |
| SexAI | ACCWGGT | 1 cut(s) | 370 |
| SfaNI | GCATC | 3 cut(s) | 259, 927, 1444 |
| SfcI | CTRYAG | 2 cut(s) | 819, 1281 |
| SinI | GGWCC | 1 cut(s) | 1081 |
| SmlI | CTYRAG | 4 cut(s) | 167, 963, 977, 1427 |
| SmoI | CTYRAG | 4 cut(s) | 167, 963, 977, 1427 |
| Sse9I | AATT | 4 cut(s) | 79, 160, 217, 403 |
| SsiI | CCGC | 7 cut(s) | 94, 462, 671, 761, 896, 1187, 1376 |
| SspMI | CTAG | 2 cut(s) | 408, 738 |
| SstI | GAGCTC | 1 cut(s) | 583 |
| StyD4I | CCNGG | 4 cut(s) | 358, 370, 718, 1567 |
| StyI | CCWWGG | 2 cut(s) | 277, 941 |
| TaaI | ACNGT | 9 cut(s) | 37, 598, 655, 1033, 1141, 1495, 1522, 1534, 1562 |
| TaiI | ACGT | 2 cut(s) | 213, 1240 |
| TaqI | TCGA | 3 cut(s) | 1134, 1358, 1702 |
| TasI | AATT | 4 cut(s) | 79, 160, 217, 403 |
| TatI | WGTACW | 2 cut(s) | 1035, 1147 |
| TauI | GCSGC | 2 cut(s) | 96, 764 |
| TfiI | GAWTC | 1 cut(s) | 181 |
| Tru1I | TTAA | 5 cut(s) | 366, 570, 783, 875, 978 |
| Tru9I | TTAA | 5 cut(s) | 366, 570, 783, 875, 978 |
| TscAI | CASTG | 4 cut(s) | 1038, 1144, 1150, 1285 |
| TseFI | GTSAC | 2 cut(s) | 211, 1528 |
| TseI | GCWGC | 3 cut(s) | 200, 590, 893 |
| Tsp45I | GTSAC | 2 cut(s) | 211, 1528 |
| TspDTI | ATGAA | 6 cut(s) | 88, 301, 632, 1168, 1227, 1556 |
| TspRI | CASTG | 4 cut(s) | 1038, 1144, 1150, 1285 |
| Tth111I | GACNNNGTC | 1 cut(s) | 1139 |
| Van91I | CCANNNNNTGG | 1 cut(s) | 1090 |
| Vha464I | CTTAAG | 1 cut(s) | 977 |
| VneI | GTGCAC | 1 cut(s) | 860 |
| VpaK11BI | GGWCC | 1 cut(s) | 1081 |
| VspI | ATTAAT | 1 cut(s) | 570 |
| XapI | RAATTY | 1 cut(s) | 403 |
| XceI | RCATGY | 1 cut(s) | 234 |
| XcmI | CCANNNNNNNNNTGG | 1 cut(s) | 1498 |
| XmiI | GTMKAC | 1 cut(s) | 20 |
| XspI | CTAG | 2 cut(s) | 408, 738 |
| Zsp2I | ATGCAT | 1 cut(s) | 1333 |