pycom11g06920

Protein NRT1 PTR FAMILY

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr11
OG0000603
Physical Location & Seq
Forward (+)
5275148 .. 5276562
1415 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom11g06920.1

Sequence Viewer

Length: 1197 bp
ATGTTTTTGGGTTGGGGATTGGGTTTCGGCATTCCAACAGTGGCAATGGCAATTGCTGTGCTGAGTTTCTTTGCTGGGACTCGGTTGTATAGGAATCAGAAGCCTGGAGGTAGTCCTCTGACGCGCATAATTCAGGTGGTTGTGGCGTCCATTAGAAAATGCAAGGTGAAAGTACCCGAAGACATTTCCCTTTTGTATGAGACTGCAGATGCAGAATCTTCCATCCAAGGAAGCCGCAAGCTTGATCACAGAAATGAATTTAGGTTAGTACAAGATGACCATTTAAAGGACTCGAAAAGCCCATGGAGACTCAGTACAGTTACCCAAGTAGAGGAGCTAAAATCAATCATGAGGCTGCTTCCCATATGGGCCACTGGTATCATCTTTGCTGCAGTCTACAATCAGATGAGCAACTTCTTTGTGTTGCAAGGCACCCTCATGGATATTCGTGTTGGAAGCTCTAGCTTGGAAATCCCAGCAGCATCTCTTTCCATCTTTGACACCCTAAGTGTAATTTTTTGGGTCCCAATATACGATCGAATCATTGTCCCAGCAGCTAGAAAATACACCGGTCACAAGAATGGCCTAACTACACTCCAGAGGATGGGCATTGGCCTCTTCATCTCCATATTCTCCATGGTCTGTGCTGCAGTTTTGGAACTCATCAGACTCCGAAGTGTTCGACAGAACAACTATTACGAATACGAGCATATGCCCATGTCAGTCTTTTGGCAAGTACCTCAGTATTTCCTCATGGGAGCTGCAGAAGTTTTCACGTTCATAGGACAGCTGGAGTTTTTCTATGATCAAGCGTCGGATGCCATGAGGAGCTTGTGTTCTGCTCTCGCACTCTCCACTGTTTCACTAGGAAACTACTTCAACTCTATTCTTGTCACCATCGTTACCAAAGCGACTACAAAGAACGGCAACCCTGGATGGATACCAAACAATTTGAACTACGGACACCTGGATTATTTCTTCTGGCTATTGGCAGTGCTGAGTGTTTTCAACCTAGGAGTATACCTCTTCATTTCCAAGAGGTATACGTATAAAAAAACTGTCGGGACTCTTGGATTGATATTGAAAAGAATTGTTACCATAAGAGGTTGTTTCTGTACTTTGATGATTTTTAGGTTTTACTTTGGTTTTCAATCACTTCACTACCAAAAAAAAAAAAAGGGCTTGCCCTACAAATAA

Protein Analysis

399

Amino Acids

45.2

Weight (kDa)

9.67

Isoelectric Point (pI)

28.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTR2 PF00854 4 - 314 2.2e-76 POT family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000603)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G54140 AT3G54140 AT5G01180 AT5G01180 AT5G01180
fragaria_vesca FvH4_3g38120 FvH4_6g11961 FvH4_6g11970 FvH4_6g11970 FvH4_6g11970 FvH4_6g11970 FvH4_7g07580 FvH4_7g07580
malus_domestica MD03G1076900.v1.1 MD04G1147500.v1.1 MD11G1081100.v1.1 MD11G1081200.v1.1 MD12G1160700.v1.1 MD12G1160900.v1.1
prunus_persica Prupe.6G061500_v2.0.a1 Prupe.6G271100_v2.0.a1 Prupe.6G271200_v2.0.a1
pyrus_communis pycom03g06080 pycom11g06920 pycom11g06940 pycom12g15250 pycom12g15270
rosa_chinensis RchiOBHm_Chr1g0343091 RchiOBHm_Chr3g0463781 RchiOBHm_Chr3g0463791 RchiOBHm_Chr5g0068471 RchiOBHm_Chr5g0068501
rosa_laevigata RLG00000024756 RLG00000024757 RLG00000029203 RLG00000035978 RLG00000035979
rosa_multiflora Rmu_co8449979.1_g000001 Rmu_sc0000313.1_g000001 Rmu_sc0003221.1_g000016 Rmu_sc0003221.1_g000025 Rmu_sc0003221.1_g000026 Rmu_sc0004796.1_g000013 Rmu_sc0005087.1_g000011 Rmu_sc0008896.1_g000017 Rmu_sc0010201.1_g000001 Rmu_sc0017585.1_g000005
rosa_roxburghii Rroxscaffold_1G00012300 Rroxscaffold_1G00012310 Rroxscaffold_1G00013040 Rroxscaffold_1G00013050 Rroxscaffold_4G00313320 Rroxscaffold_6G00416290 Rroxscaffold_6G00416300
rosa_rugosa Rorug01G0144300.1 Rorug03G0067500 Rorug03G0067600 Rorug03G0067700 Rorug05G0390200 Rorug05G0390300 Rorug05G0390400.1 Rorug05G0390500 Rorug05G0390600 Rorug05G0390700
rosa_samantha Rh1BG127900 Rh1CG150400 Rh1DG164100 Rh3AG124700 Rh3AG124800 Rh3CG131600 Rh3DG130100 Rh3DG130200 Rh5CG488500 Rh5DG480400 Rh5DG480600
rosa_wichuraiana Rw1G013410 Rw3G010540 Rw3G010550 Rw5G041900 Rw5G041920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 431
AccB7I CCANNNNNTGG 1 cut(s) 604
AccI GTMKAC 3 cut(s) 396, 1020, 1043
AccII CGCG 1 cut(s) 124
AciI CCGC 1 cut(s) 235
AcsI RAATTY 1 cut(s) 257
AcyI GRCGYC 1 cut(s) 146
AfaI GTAC 5 cut(s) 174, 270, 316, 738, 1117
AfiI CCNNNNNNNGG 3 cut(s) 286, 331, 604
AgeI ACCGGT 1 cut(s) 569
AgsI TTSAA 5 cut(s) 880, 955, 1009, 1084, 1151
AjnI CCWGG 3 cut(s) 103, 931, 966
AloI GAACNNNNNNTCC 2 cut(s) 820, 852
AluBI AGCT 8 cut(s) 241, 337, 459, 465, 557, 761, 790, 831
AluI AGCT 8 cut(s) 241, 337, 459, 465, 557, 761, 790, 831
Alw26I GTCTC 2 cut(s) 194, 301
AoxI GGCC 3 cut(s) 369, 583, 613
ApeKI GCWGC 6 cut(s) 355, 389, 479, 554, 647, 761
ApoI RAATTY 1 cut(s) 257
AsiGI ACCGGT 1 cut(s) 569
AspA2I CCTAGG 1 cut(s) 1012
AspLEI GCGC 1 cut(s) 126
AspS9I GGNCC 2 cut(s) 369, 523
AsuHPI GGTGA 2 cut(s) 178, 886
AvaII GGWCC 1 cut(s) 523
AvrII CCTAGG 1 cut(s) 1012
BanI GGYRCC 1 cut(s) 431
BbsI GAAGAC 1 cut(s) 186
BbvI GCAGC 6 cut(s) 342, 376, 491, 566, 634, 748
BccI CCATC 5 cut(s) 230, 500, 598, 905, 930
BceAI ACGGC 1 cut(s) 940
BciT130I CCWGG 3 cut(s) 105, 933, 968
BciVI GTATCC 1 cut(s) 933
BclI TGATCA 2 cut(s) 244, 805
BcoDI GTCTC 2 cut(s) 194, 301
BfaI CTAG 4 cut(s) 462, 558, 866, 1013
BfmI CTRYAG 4 cut(s) 204, 390, 648, 762
BfuI GTATCC 1 cut(s) 933
BisI GCNGC 7 cut(s) 235, 356, 390, 480, 555, 648, 762
BlnI CCTAGG 1 cut(s) 1012
BlsI GCNGC 7 cut(s) 236, 357, 391, 481, 556, 649, 763
Bme1390I CCNGG 3 cut(s) 105, 933, 968
Bme18I GGWCC 1 cut(s) 523
BmgT120I GGNCC 2 cut(s) 369, 523
BmiI GGNNCC 3 cut(s) 433, 524, 525
BmrFI CCNGG 3 cut(s) 105, 933, 968
BmsI GCATC 3 cut(s) 199, 491, 808
BpiI GAAGAC 1 cut(s) 186
BplI GAGNNNNNCTC 2 cut(s) 1008, 1040
BpmI CTGGAG 3 cut(s) 126, 581, 812
BsaAI YACGTR 1 cut(s) 1047
BsaHI GRCGYC 1 cut(s) 146
BsaJI CCNNGG 5 cut(s) 226, 302, 636, 931, 1012
BsaWI WCCGGW 1 cut(s) 569
BsaXI ACNNNNNCTCC 4 cut(s) 298, 328, 820, 850
Bsc4I CCNNNNNNNGG 3 cut(s) 286, 331, 604
Bse118I RCCGGY 1 cut(s) 569
Bse1I ACTGG 1 cut(s) 379
Bse3DI GCAATG 1 cut(s) 51
BseBI CCWGG 3 cut(s) 105, 933, 968
BseDI CCNNGG 5 cut(s) 226, 302, 636, 931, 1012
BseGI GGATG 4 cut(s) 222, 609, 823, 941
BseLI CCNNNNNNNGG 3 cut(s) 286, 331, 604
BseMI GCAATG 1 cut(s) 51
BseMII CTCAG 4 cut(s) 53, 325, 755, 989
BseNI ACTGG 1 cut(s) 379
BseRI GAGGAG 2 cut(s) 347, 841
BseXI GCAGC 6 cut(s) 342, 376, 491, 566, 634, 748
BseYI CCCAGC 3 cut(s) 74, 475, 550
Bsh1236I CGCG 1 cut(s) 124
Bsh1285I CGRYCG 1 cut(s) 538
BshFI GGCC 3 cut(s) 371, 585, 615
BshNI GGYRCC 1 cut(s) 431
BshTI ACCGGT 1 cut(s) 569
BsiEI CGRYCG 1 cut(s) 538
BsiSI CCGG 1 cut(s) 570
BslFI GGGAC 4 cut(s) 91, 509, 533, 1078
BslI CCNNNNNNNGG 3 cut(s) 286, 331, 604
BsmAI GTCTC 2 cut(s) 194, 301
BsmFI GGGAC 4 cut(s) 91, 509, 533, 1078
BsmI GAATGC 1 cut(s) 30
BsnI GGCC 3 cut(s) 371, 585, 615
Bsp143I GATC 3 cut(s) 244, 535, 805
Bsp19I CCATGG 2 cut(s) 302, 636
BspACI CCGC 1 cut(s) 235
BspANI GGCC 3 cut(s) 371, 585, 615
BspCNI CTCAG 4 cut(s) 54, 324, 754, 990
BspFNI CGCG 1 cut(s) 124
BspHI TCATGA 1 cut(s) 348
BspLI GGNNCC 3 cut(s) 433, 524, 525
BspMAI CTGCAG 4 cut(s) 208, 394, 652, 766
BspT107I GGYRCC 1 cut(s) 431
BsrDI GCAATG 1 cut(s) 51
BsrFI RCCGGY 1 cut(s) 569
BsrI ACTGG 1 cut(s) 379
BssAI RCCGGY 1 cut(s) 569
BssECI CCNNGG 5 cut(s) 226, 302, 636, 931, 1012
BssMI GATC 3 cut(s) 244, 535, 805
BssNAI GTATAC 2 cut(s) 1021, 1044
BssNI GRCGYC 1 cut(s) 146
BssT1I CCWWGG 4 cut(s) 226, 302, 636, 1012
Bst1107I GTATAC 2 cut(s) 1021, 1044
Bst2UI CCWGG 3 cut(s) 105, 933, 968
Bst4CI ACNGT 4 cut(s) 40, 319, 859, 1060
Bst6I CTCTTC 2 cut(s) 623, 1031
BstACI GRCGYC 1 cut(s) 146
BstBAI YACGTR 1 cut(s) 1047
BstC8I GCNNGC 2 cut(s) 239, 1184
BstDEI CTNAG 5 cut(s) 62, 311, 506, 741, 998
BstDSI CCRYGG 2 cut(s) 302, 636
BstF5I GGATG 4 cut(s) 222, 609, 823, 941
BstFNI CGCG 1 cut(s) 124
BstHHI GCGC 1 cut(s) 126
BstKTI GATC 3 cut(s) 247, 538, 808
BstMAI GTCTC 2 cut(s) 194, 301
BstMBI GATC 3 cut(s) 244, 535, 805
BstMCI CGRYCG 1 cut(s) 538
BstMWI GCNNNNNNNGC 1 cut(s) 818
BstNI CCWGG 3 cut(s) 105, 933, 968
BstSCI CCNGG 3 cut(s) 103, 931, 966
BstSFI CTRYAG 4 cut(s) 204, 390, 648, 762
BstSNI TACGTA 1 cut(s) 1047
BstUI CGCG 1 cut(s) 124
BstV1I GCAGC 6 cut(s) 342, 376, 491, 566, 634, 748
BstV2I GAAGAC 1 cut(s) 186
BstZ17I GTATAC 2 cut(s) 1021, 1044
BsuI GTATCC 1 cut(s) 933
BsuRI GGCC 3 cut(s) 371, 585, 615
BtgI CCRYGG 2 cut(s) 302, 636
BtsCI GGATG 4 cut(s) 222, 609, 823, 941
BtsI GCAGTG 1 cut(s) 999
BtsIMutI CAGTG 4 cut(s) 45, 372, 855, 999
Cac8I GCNNGC 2 cut(s) 239, 1184
CciI TCATGA 1 cut(s) 348
CfoI GCGC 1 cut(s) 126
Cfr10I RCCGGY 1 cut(s) 569
Cfr13I GGNCC 2 cut(s) 369, 523
CseI GACGC 3 cut(s) 130, 135, 801
Csp6I GTAC 5 cut(s) 173, 269, 315, 737, 1116
CspAI ACCGGT 1 cut(s) 569
CviAII CATG 7 cut(s) 303, 349, 439, 637, 718, 754, 823
CviQI GTAC 5 cut(s) 173, 269, 315, 737, 1116
DdeI CTNAG 5 cut(s) 62, 311, 506, 741, 998
DpnI GATC 3 cut(s) 246, 537, 807
DpnII GATC 3 cut(s) 244, 535, 805
DraI TTTAAA 1 cut(s) 285
Eam1104I CTCTTC 2 cut(s) 623, 1031
EarI CTCTTC 2 cut(s) 623, 1031
Eco105I TACGTA 1 cut(s) 1047
Eco130I CCWWGG 4 cut(s) 226, 302, 636, 1012
Eco47I GGWCC 1 cut(s) 523
EcoO109I RGGNCCY 1 cut(s) 523
EcoRII CCWGG 3 cut(s) 103, 931, 966
EcoT14I CCWWGG 4 cut(s) 226, 302, 636, 1012
ErhI CCWWGG 4 cut(s) 226, 302, 636, 1012
FaeI CATG 7 cut(s) 306, 352, 442, 640, 721, 757, 826
FaqI GGGAC 4 cut(s) 91, 509, 533, 1078
FatI CATG 7 cut(s) 302, 348, 438, 636, 717, 753, 822
FauNDI CATATG 2 cut(s) 365, 711
FbaI TGATCA 2 cut(s) 244, 805
FblI GTMKAC 3 cut(s) 396, 1020, 1043
Fnu4HI GCNGC 7 cut(s) 235, 356, 390, 480, 555, 648, 762
FokI GGATG 4 cut(s) 209, 616, 830, 948
Fsp4HI GCNGC 7 cut(s) 235, 356, 390, 480, 555, 648, 762
FspBI CTAG 4 cut(s) 462, 558, 866, 1013
GlaI GCGC 1 cut(s) 125
GluI GCNGC 7 cut(s) 235, 356, 390, 480, 555, 648, 762
GsaI CCCAGC 3 cut(s) 78, 479, 554
GsuI CTGGAG 3 cut(s) 126, 581, 812
HaeIII GGCC 3 cut(s) 371, 585, 615
HapII CCGG 1 cut(s) 570
HgaI GACGC 3 cut(s) 130, 135, 801
HhaI GCGC 1 cut(s) 126
Hin1I GRCGYC 1 cut(s) 146
Hin1II CATG 7 cut(s) 306, 352, 442, 640, 721, 757, 826
Hin6I GCGC 1 cut(s) 124
HinP1I GCGC 1 cut(s) 124
HindIII AAGCTT 1 cut(s) 239
HinfI GANTC 8 cut(s) 79, 94, 215, 290, 309, 540, 669, 1066
HpaII CCGG 1 cut(s) 570
HphI GGTGA 2 cut(s) 178, 886
Hpy166II GTNNAC 3 cut(s) 397, 1021, 1044
Hpy188I TCNGA 6 cut(s) 99, 120, 405, 668, 674, 817
Hpy188III TCNNGA 3 cut(s) 349, 598, 1063
Hpy8I GTNNAC 3 cut(s) 397, 1021, 1044
Hpy99I CGWCG 1 cut(s) 817
HpyCH4III ACNGT 4 cut(s) 40, 319, 859, 1060
HpyCH4IV ACGT 2 cut(s) 776, 1046
HpyCH4V TGCA 7 cut(s) 162, 206, 212, 392, 427, 650, 764
HpyF10VI GCNNNNNNNGC 1 cut(s) 818
HpyF3I CTNAG 5 cut(s) 62, 311, 506, 741, 998
HpySE526I ACGT 2 cut(s) 776, 1046
Hsp92I GRCGYC 1 cut(s) 146
Hsp92II CATG 7 cut(s) 306, 352, 442, 640, 721, 757, 826
HspAI GCGC 1 cut(s) 124
KflI GGGWCCC 1 cut(s) 523
Ksp22I TGATCA 2 cut(s) 244, 805
Kzo9I GATC 3 cut(s) 244, 535, 805
LmnI GCTCC 3 cut(s) 334, 758, 828
Lsp1109I GCAGC 6 cut(s) 342, 376, 491, 566, 634, 748
LweI GCATC 3 cut(s) 199, 491, 808
MaeI CTAG 4 cut(s) 462, 558, 866, 1013
MaeII ACGT 2 cut(s) 776, 1046
MaeIII GTNAC 5 cut(s) 319, 572, 892, 901, 1093
MalI GATC 3 cut(s) 246, 537, 807
MboI GATC 3 cut(s) 244, 535, 805
MboII GAAGA 5 cut(s) 191, 210, 610, 970, 1018
MfeI CAATTG 1 cut(s) 51
MluCI AATT 6 cut(s) 51, 129, 257, 513, 949, 1089
MlyI GAGTC 5 cut(s) 73, 284, 303, 663, 1060
MmeI TCCRAC 3 cut(s) 59, 433, 795
MseI TTAA 1 cut(s) 284
MslI CAYNNNNRTG 3 cut(s) 252, 437, 579
MspA1I CMGCKG 1 cut(s) 790
MspI CCGG 1 cut(s) 570
MspR9I CCNGG 3 cut(s) 105, 933, 968
MunI CAATTG 1 cut(s) 51
Mva1269I GAATGC 1 cut(s) 30
MvaI CCWGG 3 cut(s) 105, 933, 968
MvnI CGCG 1 cut(s) 124
MwoI GCNNNNNNNGC 1 cut(s) 818
NcoI CCATGG 2 cut(s) 302, 636
NdeI CATATG 2 cut(s) 365, 711
NdeII GATC 3 cut(s) 244, 535, 805
NlaIII CATG 7 cut(s) 306, 352, 442, 640, 721, 757, 826
NlaIV GGNNCC 3 cut(s) 433, 524, 525
NmuCI GTSAC 2 cut(s) 572, 892
PagI TCATGA 1 cut(s) 348
PctI GAATGC 1 cut(s) 30
PfeI GAWTC 3 cut(s) 94, 215, 540
PflMI CCANNNNNTGG 1 cut(s) 604
PinAI ACCGGT 1 cut(s) 569
PkrI GCNGC 7 cut(s) 236, 357, 391, 481, 556, 649, 763
Ple19I CGATCG 1 cut(s) 538
PleI GAGTC 5 cut(s) 73, 284, 303, 663, 1060
PpsI GAGTC 5 cut(s) 73, 284, 303, 663, 1060
Ppu21I YACGTR 1 cut(s) 1047
PpuMI RGGWCCY 1 cut(s) 523
Psp5II RGGWCCY 1 cut(s) 523
Psp6I CCWGG 3 cut(s) 103, 931, 966
PspFI CCCAGC 3 cut(s) 74, 475, 550
PspGI CCWGG 3 cut(s) 103, 931, 966
PspN4I GGNNCC 3 cut(s) 433, 524, 525
PspPI GGNCC 2 cut(s) 369, 523
PspPPI RGGWCCY 1 cut(s) 523
PsrI GAACNNNNNNTAC 2 cut(s) 680, 712
PstI CTGCAG 4 cut(s) 208, 394, 652, 766
PvuI CGATCG 1 cut(s) 538
PvuII CAGCTG 1 cut(s) 790
RsaI GTAC 5 cut(s) 174, 270, 316, 738, 1117
RsaNI GTAC 5 cut(s) 173, 269, 315, 737, 1116
RseI CAYNNNNRTG 3 cut(s) 252, 437, 579
SaqAI TTAA 1 cut(s) 284
SatI GCNGC 7 cut(s) 235, 356, 390, 480, 555, 648, 762
Sau3AI GATC 3 cut(s) 244, 535, 805
Sau96I GGNCC 2 cut(s) 369, 523
SchI GAGTC 5 cut(s) 73, 284, 303, 663, 1060
ScrFI CCNGG 3 cut(s) 105, 933, 968
SfaNI GCATC 3 cut(s) 199, 491, 808
SfcI CTRYAG 4 cut(s) 204, 390, 648, 762
SinI GGWCC 1 cut(s) 523
SmiMI CAYNNNNRTG 3 cut(s) 252, 437, 579
SnaBI TACGTA 1 cut(s) 1047
Sse9I AATT 6 cut(s) 51, 129, 257, 513, 949, 1089
SsiI CCGC 1 cut(s) 235
SspMI CTAG 4 cut(s) 462, 558, 866, 1013
StyD4I CCNGG 3 cut(s) 103, 931, 966
StyI CCWWGG 4 cut(s) 226, 302, 636, 1012
TaaI ACNGT 4 cut(s) 40, 319, 859, 1060
TaiI ACGT 2 cut(s) 779, 1049
TaqI TCGA 3 cut(s) 293, 538, 682
TasI AATT 6 cut(s) 51, 129, 257, 513, 949, 1089
TatI WGTACW 3 cut(s) 268, 314, 1115
TauI GCSGC 1 cut(s) 237
TfiI GAWTC 3 cut(s) 94, 215, 540
Tru1I TTAA 1 cut(s) 284
Tru9I TTAA 1 cut(s) 284
TscAI CASTG 4 cut(s) 45, 379, 862, 999
TseFI GTSAC 2 cut(s) 572, 892
TseI GCWGC 6 cut(s) 355, 389, 479, 554, 647, 761
Tsp45I GTSAC 2 cut(s) 572, 892
TspDTI ATGAA 4 cut(s) 270, 610, 769, 1018
TspGWI ACGGA 1 cut(s) 975
TspRI CASTG 4 cut(s) 45, 379, 862, 999
Van91I CCANNNNNTGG 1 cut(s) 604
VpaK11BI GGWCC 1 cut(s) 523
XapI RAATTY 1 cut(s) 257
XcmI CCANNNNNNNNNTGG 1 cut(s) 634
XmaJI CCTAGG 1 cut(s) 1012
XmiI GTMKAC 3 cut(s) 396, 1020, 1043
XspI CTAG 4 cut(s) 462, 558, 866, 1013
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.