AT3G58660

ribosomal

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0004127
Physical Location & Seq
Forward (+)
21701520 .. 21703114
1595 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G58660.1

Sequence Viewer

Length: 1341 bp
ATGACCACCGCAGTTTCGCCGCCGCCGCCGCAGGAGCAGCAACTTGTTCACGCTTCGCAGACTTCAAGAGTGAGTCCCAAGACAGTGGAGAGCGCCCTCAATGGTCTTATCAATTGGAGAAGCGACAAATCAAAGACAGAGAAGCCTCAACTTCTCGAAGAAGACGAGCTCGTCTACCTTTTCGTTACTTTGAAGAAGATTCCACAGAAGACCCGTACGAATGCTTACCGTATCCCTTTACCTCATCCTCTTATCAACCCGACGGTCGATTCGCCGGAGATTTGTCTCATCATCGACGACAGACCTAAAAGTGGTCTCACCAAAGATGATGCGATGAAGAAGATCAAATCTGAGAATATTCCAATCACCAAGGTTATTAAGCTTTCGAAACTGAAGAGTGATTACAAGGCGTTCGAAGCGAAGAGGAAACTCTGCGACTCTTACGACATGTTTTTCACTGATCGGAGGATCATTCCTCTGCTGCCGAGGGTGATTGGGAAGAAGTTCTTTACGAGCAAGAAGATTCCGGTGGCGCTTGATTTGAAGCACAGGAACTGGAAACATCAGATAGAGAAAGCTTGTGGATCTGCTATGTTCTTCATAAGGACTGGGACGTGTAGTGTCATTAAGGTTGGGAAATTGTCTATGGATATTTGTGAAATTACTGAGAATGTGATGGCGACATTGAATGGACTAGTTGAGTTCTTGCCTAATAAGTGGACGTACGTTAGGTCTTTGCATTTGAAGTTGTCTGAGAGTTTAGCTTTGCCTATATATCAGTCTGTTCCTGATTTGAAGCTTAAAATCGATGCTTTTGGGTCTGGAAAGAGTGTTGTTCAGGAGGAGAAGGAAGGTGAAGTTGAAGAAGTTGTTGCTGTTGATGGTGGAGAGAAGAGTGTTACAGGGAAGGGAAAGAAGAAGAAAGGTAGGATTCACGAGGTTAGGTATATGGATAGTAATGTAGCTGAGGTGCTTGATGAGGATGAGATTGGTGGTGACGAGGATAATAATGAGATTGTTGAGTCTGAGGAGAAGAAGATGAAGAAGAGGAAGAAGGAGGTAAGTGAAGTTGCAGAGAGTGAGAAGCCGATGAAGAAAGCTGCCAAAGGGAAGTTGAAACCTGATGTTGAGAAACCGATGAAGAAAGCTGCCAAAGGGAAGTCGAAACCCGATGTTGAGAAACCTATGCAGAAAGCTGCGGTCAAGGGGAAAGTTAAACCAGATGTTGTGAAGCTGGAGAAGAGCAAGGACGGCTTAAAGGCGAAAAAGACAACAATGGTTGCGAAAGACGAATCTGGTGGTGGATTGAAGCCGAAGAGAAAGAGTGTGCTTAGGAAATGA

Protein Analysis

446

Amino Acids

49.97

Weight (kDa)

9.57

Isoelectric Point (pI)

32.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L1 PF00687 52 - 253 1.7e-47 Ribosomal protein L1p/L10e family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 170
AccI GTMKAC 1 cut(s) 174
AciI CCGC 6 cut(s) 9, 20, 23, 26, 29, 1199
AclWI GGATC 2 cut(s) 476, 592
AcuI CTGAAG 1 cut(s) 413
AfaI GTAC 2 cut(s) 217, 725
AfiI CCNNNNNNNGG 1 cut(s) 311
AflIII ACRYGT 2 cut(s) 447, 614
AgsI TTSAA 9 cut(s) 66, 193, 544, 688, 745, 796, 863, 1117, 1309
AhlI ACTAGT 1 cut(s) 694
AjiI CACGTC 1 cut(s) 615
Alw21I GWGCWC 1 cut(s) 171
Alw26I GTCTC 2 cut(s) 290, 320
AlwI GGATC 2 cut(s) 476, 592
AlwNI CAGNNNCTG 1 cut(s) 555
ApeKI GCWGC 5 cut(s) 37, 481, 1100, 1148, 1196
ArsI GACNNNNNNTTYG 2 cut(s) 253, 285
Asp700I GAANNNNTTC 1 cut(s) 503
AspLEI GCGC 2 cut(s) 95, 535
AsuHPI GGTGA 5 cut(s) 310, 358, 502, 866, 1007
AsuII TTCGAA 2 cut(s) 386, 414
BanII GRGCYC 1 cut(s) 171
BarI GAAGNNNNNNTAC 2 cut(s) 386, 418
BauI CACGAG 1 cut(s) 935
BbsI GAAGAC 2 cut(s) 168, 215
Bbv12I GWGCWC 1 cut(s) 171
BbvCI CCTCAGC 1 cut(s) 966
BbvI GCAGC 5 cut(s) 49, 468, 1087, 1135, 1183
BccI CCATC 2 cut(s) 670, 875
BceAI ACGGC 1 cut(s) 1267
BciVI GTATCC 1 cut(s) 242
BcoDI GTCTC 2 cut(s) 290, 320
BcuI ACTAGT 1 cut(s) 694
BfaI CTAG 1 cut(s) 695
BfoI RGCGCY 2 cut(s) 96, 536
BfuI GTATCC 1 cut(s) 242
BisI GCNGC 9 cut(s) 20, 23, 26, 29, 38, 482, 1101, 1149, 1197
BlsI GCNGC 9 cut(s) 21, 24, 27, 30, 39, 483, 1102, 1150, 1198
BmgBI CACGTC 1 cut(s) 615
BmrI ACTGGG 1 cut(s) 618
BmsI GCATC 2 cut(s) 319, 799
BmuI ACTGGG 1 cut(s) 618
BpiI GAAGAC 2 cut(s) 168, 215
BpmI CTGGAG 1 cut(s) 1256
Bpu10I CCTNAGC 2 cut(s) 966, 1331
Bpu14I TTCGAA 2 cut(s) 386, 414
Bsa29I ATCGAT 1 cut(s) 807
BsaI GGTCTC 1 cut(s) 320
BsaJI CCNNGG 2 cut(s) 369, 485
BsaWI WCCGGW 1 cut(s) 526
Bsc4I CCNNNNNNNGG 1 cut(s) 311
Bse1I ACTGG 2 cut(s) 560, 613
BseCI ATCGAT 1 cut(s) 807
BseDI CCNNGG 2 cut(s) 369, 485
BseGI GGATG 2 cut(s) 244, 988
BseLI CCNNNNNNNGG 1 cut(s) 311
BseMII CTCAG 5 cut(s) 342, 657, 744, 957, 1017
BseNI ACTGG 2 cut(s) 560, 613
BseRI GAGGAG 2 cut(s) 857, 1043
BseXI GCAGC 5 cut(s) 49, 468, 1087, 1135, 1183
Bsh1285I CGRYCG 1 cut(s) 267
BshVI ATCGAT 1 cut(s) 807
BsiEI CGRYCG 1 cut(s) 267
BsiHKAI GWGCWC 1 cut(s) 171
BsiSI CCGG 2 cut(s) 275, 527
BsiWI CGTACG 2 cut(s) 215, 723
BslFI GGGAC 2 cut(s) 60, 625
BslI CCNNNNNNNGG 1 cut(s) 311
BsmAI GTCTC 2 cut(s) 290, 320
BsmFI GGGAC 2 cut(s) 60, 625
BsmI GAATGC 1 cut(s) 226
Bso31I GGTCTC 1 cut(s) 320
Bsp119I TTCGAA 2 cut(s) 386, 414
Bsp1286I GDGCHC 1 cut(s) 171
Bsp143I GATC 4 cut(s) 342, 460, 468, 584
BspACI CCGC 6 cut(s) 9, 20, 23, 26, 29, 1199
BspCNI CTCAG 5 cut(s) 343, 658, 745, 958, 1018
BspDI ATCGAT 1 cut(s) 807
BspPI GGATC 2 cut(s) 476, 592
BspQI GCTCTTC 1 cut(s) 1235
BspT104I TTCGAA 2 cut(s) 386, 414
BspTNI GGTCTC 1 cut(s) 320
BsrI ACTGG 2 cut(s) 560, 613
BssECI CCNNGG 2 cut(s) 369, 485
BssMI GATC 4 cut(s) 342, 460, 468, 584
BssSI CACGAG 1 cut(s) 935
BssT1I CCWWGG 1 cut(s) 369
Bst2BI CACGAG 1 cut(s) 935
Bst4CI ACNGT 3 cut(s) 85, 230, 265
Bst6I CTCTTC 6 cut(s) 389, 416, 887, 1040, 1235, 1310
BstBI TTCGAA 2 cut(s) 386, 414
BstDEI CTNAG 6 cut(s) 351, 666, 753, 966, 1026, 1331
BstF5I GGATG 2 cut(s) 244, 988
BstH2I RGCGCY 2 cut(s) 96, 536
BstHHI GCGC 2 cut(s) 95, 535
BstKTI GATC 4 cut(s) 345, 463, 471, 587
BstMAI GTCTC 2 cut(s) 290, 320
BstMBI GATC 4 cut(s) 342, 460, 468, 584
BstMCI CGRYCG 1 cut(s) 267
BstMWI GCNNNNNNNGC 6 cut(s) 25, 28, 34, 37, 416, 1251
BstNSI RCATGY 1 cut(s) 451
BstV1I GCAGC 5 cut(s) 49, 468, 1087, 1135, 1183
BstV2I GAAGAC 2 cut(s) 168, 215
BstX2I RGATCY 1 cut(s) 584
BstXI CCANNNNNNTGG 1 cut(s) 85
BstYI RGATCY 1 cut(s) 584
Bsu15I ATCGAT 1 cut(s) 807
BsuI GTATCC 1 cut(s) 242
BsuTUI ATCGAT 1 cut(s) 807
BtgZI GCGATG 1 cut(s) 347
BtrI CACGTC 1 cut(s) 615
BtsCI GGATG 2 cut(s) 244, 988
BtsIMutI CAGTG 2 cut(s) 90, 456
CaiI CAGNNNCTG 1 cut(s) 555
CfoI GCGC 2 cut(s) 95, 535
ClaI ATCGAT 1 cut(s) 807
Csp6I GTAC 2 cut(s) 216, 724
CviAII CATG 1 cut(s) 448
CviQI GTAC 2 cut(s) 216, 724
DdeI CTNAG 6 cut(s) 351, 666, 753, 966, 1026, 1331
DpnI GATC 4 cut(s) 344, 462, 470, 586
DpnII GATC 4 cut(s) 342, 460, 468, 584
DrdI GACNNNNNNGTC 1 cut(s) 170
DseDI GACNNNNNNGTC 1 cut(s) 170
Eam1104I CTCTTC 6 cut(s) 389, 416, 887, 1040, 1235, 1310
EarI CTCTTC 6 cut(s) 389, 416, 887, 1040, 1235, 1310
Ecl136II GAGCTC 1 cut(s) 169
Eco130I CCWWGG 1 cut(s) 369
Eco24I GRGCYC 1 cut(s) 171
Eco31I GGTCTC 1 cut(s) 320
Eco53kI GAGCTC 1 cut(s) 169
Eco57I CTGAAG 1 cut(s) 413
EcoICRI GAGCTC 1 cut(s) 169
EcoT14I CCWWGG 1 cut(s) 369
EcoT38I GRGCYC 1 cut(s) 171
ErhI CCWWGG 1 cut(s) 369
FaeI CATG 1 cut(s) 451
FaiI YATR 9 cut(s) 449, 593, 602, 647, 773, 775, 948, 950, 1187
FalI AAGNNNNNCTT 4 cut(s) 491, 523, 1238, 1270
FaqI GGGAC 2 cut(s) 60, 625
FatI CATG 1 cut(s) 447
FblI GTMKAC 1 cut(s) 174
Fnu4HI GCNGC 9 cut(s) 20, 23, 26, 29, 38, 482, 1101, 1149, 1197
FokI GGATG 2 cut(s) 231, 995
FriOI GRGCYC 1 cut(s) 171
Fsp4HI GCNGC 9 cut(s) 20, 23, 26, 29, 38, 482, 1101, 1149, 1197
FspBI CTAG 1 cut(s) 695
GlaI GCGC 2 cut(s) 94, 534
GluI GCNGC 9 cut(s) 20, 23, 26, 29, 38, 482, 1101, 1149, 1197
GsuI CTGGAG 1 cut(s) 1256
HaeII RGCGCY 2 cut(s) 96, 536
HapII CCGG 2 cut(s) 275, 527
HhaI GCGC 2 cut(s) 95, 535
Hin1II CATG 1 cut(s) 451
Hin6I GCGC 2 cut(s) 93, 533
HinP1I GCGC 2 cut(s) 93, 533
HindIII AAGCTT 3 cut(s) 380, 576, 797
HinfI GANTC 8 cut(s) 73, 199, 269, 437, 523, 931, 1022, 1292
HpaII CCGG 2 cut(s) 275, 527
HphI GGTGA 5 cut(s) 310, 358, 502, 866, 1007
Hpy166II GTNNAC 3 cut(s) 49, 175, 720
Hpy188I TCNGA 5 cut(s) 352, 465, 567, 754, 1027
Hpy188III TCNNGA 6 cut(s) 66, 155, 788, 822, 839, 935
Hpy8I GTNNAC 3 cut(s) 49, 175, 720
Hpy99I CGWCG 2 cut(s) 265, 299
HpyAV CCTTC 4 cut(s) 841, 845, 901, 1048
HpyCH4III ACNGT 3 cut(s) 85, 230, 265
HpyCH4IV ACGT 3 cut(s) 614, 722, 726
HpyCH4V TGCA 3 cut(s) 739, 1073, 1189
HpyF10VI GCNNNNNNNGC 6 cut(s) 25, 28, 34, 37, 416, 1251
HpyF3I CTNAG 6 cut(s) 351, 666, 753, 966, 1026, 1331
HpySE526I ACGT 3 cut(s) 614, 722, 726
Hsp92II CATG 1 cut(s) 451
HspAI GCGC 2 cut(s) 93, 533
Kzo9I GATC 4 cut(s) 342, 460, 468, 584
LguI GCTCTTC 1 cut(s) 1235
LmnI GCTCC 1 cut(s) 34
Lsp1109I GCAGC 5 cut(s) 49, 468, 1087, 1135, 1183
LweI GCATC 2 cut(s) 319, 799
MaeI CTAG 1 cut(s) 695
MaeII ACGT 3 cut(s) 614, 722, 726
MaeIII GTNAC 3 cut(s) 184, 898, 995
MalI GATC 4 cut(s) 344, 462, 470, 586
MboI GATC 4 cut(s) 342, 460, 468, 584
MfeI CAATTG 1 cut(s) 112
MflI RGATCY 1 cut(s) 584
MhlI GDGCHC 1 cut(s) 171
MluCI AATT 3 cut(s) 112, 638, 660
MlyI GAGTC 3 cut(s) 82, 431, 1031
MroXI GAANNNNTTC 1 cut(s) 503
MseI TTAA 5 cut(s) 378, 627, 801, 1215, 1256
MspI CCGG 2 cut(s) 275, 527
MunI CAATTG 1 cut(s) 112
Mva1269I GAATGC 1 cut(s) 226
MwoI GCNNNNNNNGC 6 cut(s) 25, 28, 34, 37, 416, 1251
NdeII GATC 4 cut(s) 342, 460, 468, 584
NlaIII CATG 1 cut(s) 451
NmeAIII GCCGAG 1 cut(s) 510
NmuCI GTSAC 1 cut(s) 995
NspI RCATGY 1 cut(s) 451
NspV TTCGAA 2 cut(s) 386, 414
PciI ACATGT 1 cut(s) 447
PciSI GCTCTTC 1 cut(s) 1235
PcsI WCGNNNNNNNCGW 1 cut(s) 162
PctI GAATGC 1 cut(s) 226
PdmI GAANNNNTTC 1 cut(s) 503
PfeI GAWTC 5 cut(s) 199, 269, 523, 931, 1292
Pfl23II CGTACG 2 cut(s) 215, 723
PkrI GCNGC 9 cut(s) 21, 24, 27, 30, 39, 483, 1102, 1150, 1198
PleI GAGTC 3 cut(s) 81, 431, 1030
PpsI GAGTC 3 cut(s) 81, 431, 1030
PscI ACATGT 1 cut(s) 447
Psp124BI GAGCTC 1 cut(s) 171
PspLI CGTACG 2 cut(s) 215, 723
PstNI CAGNNNCTG 1 cut(s) 555
PsuI RGATCY 1 cut(s) 584
RsaI GTAC 2 cut(s) 217, 725
RsaNI GTAC 2 cut(s) 216, 724
SacI GAGCTC 1 cut(s) 171
SapI GCTCTTC 1 cut(s) 1235
SaqAI TTAA 5 cut(s) 378, 627, 801, 1215, 1256
SatI GCNGC 9 cut(s) 20, 23, 26, 29, 38, 482, 1101, 1149, 1197
Sau3AI GATC 4 cut(s) 342, 460, 468, 584
SchI GAGTC 3 cut(s) 82, 431, 1031
SduI GDGCHC 1 cut(s) 171
SfaNI GCATC 2 cut(s) 319, 799
SfuI TTCGAA 2 cut(s) 386, 414
SpeI ACTAGT 1 cut(s) 694
Sse9I AATT 3 cut(s) 112, 638, 660
SsiI CCGC 6 cut(s) 9, 20, 23, 26, 29, 1199
SspI AATATT 1 cut(s) 358
SspMI CTAG 1 cut(s) 695
SstI GAGCTC 1 cut(s) 171
StyI CCWWGG 1 cut(s) 369
TaaI ACNGT 3 cut(s) 85, 230, 265
TaiI ACGT 3 cut(s) 617, 725, 729
TaqI TCGA 7 cut(s) 156, 267, 294, 386, 414, 807, 1163
TasI AATT 3 cut(s) 112, 638, 660
TauI GCSGC 4 cut(s) 22, 25, 28, 31
TfiI GAWTC 5 cut(s) 199, 269, 523, 931, 1292
Tru1I TTAA 5 cut(s) 378, 627, 801, 1215, 1256
Tru9I TTAA 5 cut(s) 378, 627, 801, 1215, 1256
TscAI CASTG 2 cut(s) 90, 463
TseFI GTSAC 1 cut(s) 995
TseI GCWGC 5 cut(s) 37, 481, 1100, 1148, 1196
Tsp45I GTSAC 1 cut(s) 995
TspDTI ATGAA 5 cut(s) 350, 589, 1055, 1106, 1154
TspRI CASTG 2 cut(s) 90, 463
XceI RCATGY 1 cut(s) 451
XmiI GTMKAC 1 cut(s) 174
XmnI GAANNNNTTC 1 cut(s) 503
XspI CTAG 1 cut(s) 695
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.