MD17G1139700.v1.1

Ribosomal L1 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0004127
Physical Location & Seq
Forward (+)
12558421 .. 12559836
1416 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1139700.v1.1.491

Sequence Viewer

Length: 1416 bp
ATGGCCGCCACCACTCCAGCGCCTCCACCTTCCGCCACAAAACCCACCAAATCGATCGCCCAAACTGTGCGGAAAGCCGTAAACGCCCTCATCAAATGGCGGAACGACAAGCTGCAGACCCAGAACCCCGATCTCCTGGAATCCGACGAGTTCGCCTACCTAGTTCTCACCCTTAAGAAAATCCCGCCAAAGGGTCGCGTCAACGCCTACAAAATCCCCCTCCCAAATCCCCTCCACTCCCAACTCTCCGAGCTCTGCCTGATATACGACGACGGACCCAAGTCCGACCTCACCAAGGACTTCATCGAGAAGAAAATCAAGGCCGAAAACATACCCGTGGCGAAAATCTTGAAGCTTTCGAAGCTCAAGAGCGATTACGTGCCCTTTGAGGCCAAGAGGAAGCTGATGTATTCTTATGATATGTTTCTGGCTGATAAGCGGATTGTGCCGTTGCTGCCGAAGTATTTGGGGAAGCACTTCTTTAAGAAGAAGAAGATTCCGGTGCCGGTGGACTTGCAGCACAAGAATTGGAAGGAGCAGGTGGATAAGATTTGTGGGTCGGCTTTGTTGTTCTTGAGTACAGGGACGTGTAGTGTGGTGAGGGTTGCGAAAGTTTCGATGAGCATTGATGAGATTGTGGAGAATGCGGTTGCGGCGATTAATGGGATAGTGGAGATTGTGCCGAAAAATTGGGGAGGTGTGAGGTCGTTTCATTTGAAGTTGTTTGAATCGCTTGCGTTGCCGGTTTATCAGGCGGTTCCAGATGTGACATTGAAGATTGAGGGAGAGAAGGGTGTTGTGGAAGGTGAGAAGGAGGTGAAAGAGGTTGATAAGAGTGAGAGCAAGGATTTGAAGAGTGAGAAGAAGAGTAAGAAGAAGGGTAGGATTCATGAAGTGAGGTATTTGGATAGCCATGTTGGCGAGGTGCTTGATGATGATGATTTCGGTATTGTTGGTGGTGTGGACATTGGGGAAGGTAAGCAAAGCGAGAATGATGAACCAGACAGTGGCGAATTGGGGAAGAAGAAGAGGAAGAAAGAAAAAGTTGCTGGTGAGTTGAAGGGTGAGAAAAGGTTGAAGAAATCAGCTAAGGCGAAAGATGATACTGAGTTAAAGGGGGAAAAGGTTATTGGTGAGAAGAAGTTGAAAAGGTCGGCGAAGGCAGGAGAAGAAGATGGTGCCACTGTCAATCATAAGAAAGATGGGTTGTCCTTGAAAGAGAAGAAAAAAAATCTTACAAAGAAGAAAGTAGATGATTCGTCGGCTCGAGGAAAAGAGTCTATTGGAAAGAAAGAGAAGAGAAAGAGTAAAGAGTCTGTTGGAAAGAAGGAGAAGAGGAAGAGTGAAGATGAAAAGTTGAAGAGCACAGAAGCAAACCTGAAGAAGGCCAAGAGAAGTAAGAAAGCAGCAGAGTGA

Protein Analysis

472

Amino Acids

52.52

Weight (kDa)

9.63

Isoelectric Point (pI)

28.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L1 PF00687 48 - 243 2e-46 Ribosomal protein L1p/L10e family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 529
Acc36I ACCTGC 1 cut(s) 529
AccB1I GGYRCC 2 cut(s) 502, 1178
AccB7I CCANNNNNTGG 1 cut(s) 1007
AccII CGCG 1 cut(s) 198
AciI CCGC 9 cut(s) 6, 33, 70, 100, 185, 439, 647, 653, 755
AcoI YGGCCR 1 cut(s) 3
AcuI CTGAAG 1 cut(s) 1400
AfaI GTAC 1 cut(s) 580
AfiI CCNNNNNNNGG 5 cut(s) 190, 191, 295, 1007, 1384
AflII CTTAAG 1 cut(s) 173
AflIII ACRYGT 1 cut(s) 587
AjiI CACGTC 1 cut(s) 588
AjnI CCWGG 1 cut(s) 135
AjuI GAANNNNNNNTTGG 2 cut(s) 1113, 1145
AluBI AGCT 6 cut(s) 112, 253, 355, 364, 403, 1088
AluI AGCT 6 cut(s) 112, 253, 355, 364, 403, 1088
Alw21I GWGCWC 2 cut(s) 255, 1367
Ama87I CYCGRG 1 cut(s) 1266
AoxI GGCC 4 cut(s) 3, 321, 390, 1386
ApeKI GCWGC 4 cut(s) 112, 454, 517, 1406
AseI ATTAAT 1 cut(s) 660
Asp700I GAANNNNTTC 1 cut(s) 476
AspLEI GCGC 1 cut(s) 22
AspS9I GGNCC 1 cut(s) 275
AsuHPI GGTGA 8 cut(s) 160, 283, 610, 818, 829, 1064, 1076, 1145
AsuII TTCGAA 1 cut(s) 359
AvaI CYCGRG 1 cut(s) 1266
AvaII GGWCC 1 cut(s) 275
BaeGI GKGCMC 1 cut(s) 384
BanI GGYRCC 2 cut(s) 502, 1178
BanII GRGCYC 1 cut(s) 255
Bbv12I GWGCWC 2 cut(s) 255, 1367
BbvI GCAGC 3 cut(s) 99, 441, 529
BccI CCATC 2 cut(s) 1169, 1196
BceAI ACGGC 2 cut(s) 62, 433
BciT130I CCWGG 1 cut(s) 137
BfaI CTAG 1 cut(s) 161
BfmI CTRYAG 1 cut(s) 113
BfoI RGCGCY 1 cut(s) 23
BfrI CTTAAG 1 cut(s) 173
BfuAI ACCTGC 1 cut(s) 529
BglI GCCNNNNNGGC 1 cut(s) 918
BisI GCNGC 6 cut(s) 6, 113, 455, 518, 654, 1407
BlsI GCNGC 6 cut(s) 7, 114, 456, 519, 655, 1408
Bme1390I CCNGG 1 cut(s) 137
Bme18I GGWCC 1 cut(s) 275
BmeT110I CYCGRG 1 cut(s) 1266
BmgBI CACGTC 1 cut(s) 588
BmgT120I GGNCC 1 cut(s) 275
BmiI GGNNCC 4 cut(s) 277, 504, 759, 1180
BmrFI CCNGG 1 cut(s) 137
BoxI GACNNNNGTC 1 cut(s) 280
Bpu10I CCTNAGC 1 cut(s) 1089
Bpu14I TTCGAA 1 cut(s) 359
BpuEI CTTGAG 2 cut(s) 350, 595
Bsa29I ATCGAT 1 cut(s) 53
BsaAI YACGTR 1 cut(s) 379
BsaJI CCNNGG 2 cut(s) 294, 336
BsaWI WCCGGW 1 cut(s) 499
Bsc4I CCNNNNNNNGG 5 cut(s) 190, 191, 295, 1007, 1384
Bse118I RCCGGY 2 cut(s) 505, 742
BseBI CCWGG 1 cut(s) 137
BseCI ATCGAT 1 cut(s) 53
BseDI CCNNGG 2 cut(s) 294, 336
BseLI CCNNNNNNNGG 5 cut(s) 190, 191, 295, 1007, 1384
BseMII CTCAG 1 cut(s) 1098
BseSI GKGCMC 1 cut(s) 384
BseXI GCAGC 3 cut(s) 99, 441, 529
Bsh1236I CGCG 1 cut(s) 198
Bsh1285I CGRYCG 1 cut(s) 57
BshFI GGCC 4 cut(s) 5, 323, 392, 1388
BshNI GGYRCC 2 cut(s) 502, 1178
BshVI ATCGAT 1 cut(s) 53
BsiEI CGRYCG 1 cut(s) 57
BsiHKAI GWGCWC 2 cut(s) 255, 1367
BsiHKCI CYCGRG 1 cut(s) 1266
BsiSI CCGG 3 cut(s) 500, 506, 743
BslFI GGGAC 1 cut(s) 598
BslI CCNNNNNNNGG 5 cut(s) 190, 191, 295, 1007, 1384
BsmFI GGGAC 1 cut(s) 598
BsmI GAATGC 1 cut(s) 649
BsnI GGCC 4 cut(s) 5, 323, 392, 1388
BsoBI CYCGRG 1 cut(s) 1266
Bsp119I TTCGAA 1 cut(s) 359
Bsp1286I GDGCHC 3 cut(s) 255, 384, 1367
Bsp143I GATC 2 cut(s) 54, 130
BspACI CCGC 9 cut(s) 6, 33, 70, 100, 185, 439, 647, 653, 755
BspANI GGCC 4 cut(s) 5, 323, 392, 1388
BspCNI CTCAG 1 cut(s) 1099
BspDI ATCGAT 1 cut(s) 53
BspFNI CGCG 1 cut(s) 198
BspHI TCATGA 1 cut(s) 889
BspLI GGNNCC 4 cut(s) 277, 504, 759, 1180
BspMAI CTGCAG 1 cut(s) 117
BspMI ACCTGC 1 cut(s) 529
BspQI GCTCTTC 1 cut(s) 1355
BspT104I TTCGAA 1 cut(s) 359
BspT107I GGYRCC 2 cut(s) 502, 1178
BspTI CTTAAG 1 cut(s) 173
BsrFI RCCGGY 2 cut(s) 505, 742
BssAI RCCGGY 2 cut(s) 505, 742
BssECI CCNNGG 2 cut(s) 294, 336
BssMI GATC 2 cut(s) 54, 130
BssT1I CCWWGG 1 cut(s) 294
Bst2UI CCWGG 1 cut(s) 137
Bst4CI ACNGT 3 cut(s) 67, 1007, 1186
Bst6I CTCTTC 7 cut(s) 848, 860, 1022, 1292, 1328, 1334, 1355
BstAFI CTTAAG 1 cut(s) 173
BstBAI YACGTR 1 cut(s) 379
BstBI TTCGAA 1 cut(s) 359
BstC8I GCNNGC 1 cut(s) 735
BstDEI CTNAG 2 cut(s) 1089, 1107
BstDSI CCRYGG 1 cut(s) 336
BstENI CCTNNNNNAGG 2 cut(s) 293, 1382
BstFNI CGCG 1 cut(s) 198
BstH2I RGCGCY 1 cut(s) 23
BstHHI GCGC 1 cut(s) 22
BstKTI GATC 2 cut(s) 57, 133
BstMBI GATC 2 cut(s) 54, 130
BstMCI CGRYCG 1 cut(s) 57
BstMWI GCNNNNNNNGC 7 cut(s) 83, 361, 445, 454, 653, 739, 918
BstNI CCWGG 1 cut(s) 137
BstPAI GACNNNNGTC 1 cut(s) 280
BstSCI CCNGG 1 cut(s) 135
BstSFI CTRYAG 1 cut(s) 113
BstSLI GKGCMC 1 cut(s) 384
BstUI CGCG 1 cut(s) 198
BstV1I GCAGC 3 cut(s) 99, 441, 529
Bsu15I ATCGAT 1 cut(s) 53
BsuRI GGCC 4 cut(s) 5, 323, 392, 1388
BsuTUI ATCGAT 1 cut(s) 53
BtgI CCRYGG 1 cut(s) 336
BtrI CACGTC 1 cut(s) 588
BtsIMutI CAGTG 2 cut(s) 1012, 1182
BveI ACCTGC 1 cut(s) 529
Cac8I GCNNGC 1 cut(s) 735
CciI TCATGA 1 cut(s) 889
CfoI GCGC 1 cut(s) 22
Cfr10I RCCGGY 2 cut(s) 505, 742
Cfr13I GGNCC 1 cut(s) 275
ClaI ATCGAT 1 cut(s) 53
CseI GACGC 1 cut(s) 187
Csp6I GTAC 1 cut(s) 579
CviAII CATG 2 cut(s) 890, 914
CviQI GTAC 1 cut(s) 579
DdeI CTNAG 2 cut(s) 1089, 1107
DpnI GATC 2 cut(s) 56, 132
DpnII GATC 2 cut(s) 54, 130
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 7 cut(s) 848, 860, 1022, 1292, 1328, 1334, 1355
EarI CTCTTC 7 cut(s) 848, 860, 1022, 1292, 1328, 1334, 1355
EciI GGCGGA 2 cut(s) 22, 115
Ecl136II GAGCTC 1 cut(s) 253
Eco130I CCWWGG 1 cut(s) 294
Eco24I GRGCYC 1 cut(s) 255
Eco47I GGWCC 1 cut(s) 275
Eco53kI GAGCTC 1 cut(s) 253
Eco57I CTGAAG 1 cut(s) 1400
Eco88I CYCGRG 1 cut(s) 1266
EcoICRI GAGCTC 1 cut(s) 253
EcoNI CCTNNNNNAGG 2 cut(s) 293, 1382
EcoRII CCWGG 1 cut(s) 135
EcoT14I CCWWGG 1 cut(s) 294
EcoT38I GRGCYC 1 cut(s) 255
ErhI CCWWGG 1 cut(s) 294
FaeI CATG 2 cut(s) 893, 917
FaiI YATR 7 cut(s) 265, 332, 417, 422, 891, 915, 1194
FalI AAGNNNNNCTT 2 cut(s) 464, 496
FaqI GGGAC 1 cut(s) 598
FatI CATG 2 cut(s) 889, 913
FauI CCCGC 1 cut(s) 192
Fnu4HI GCNGC 6 cut(s) 6, 113, 455, 518, 654, 1407
FriOI GRGCYC 1 cut(s) 255
Fsp4HI GCNGC 6 cut(s) 6, 113, 455, 518, 654, 1407
FspBI CTAG 1 cut(s) 161
GlaI GCGC 1 cut(s) 21
GluI GCNGC 6 cut(s) 6, 113, 455, 518, 654, 1407
HaeII RGCGCY 1 cut(s) 23
HaeIII GGCC 4 cut(s) 5, 323, 392, 1388
HapII CCGG 3 cut(s) 500, 506, 743
HgaI GACGC 1 cut(s) 187
HhaI GCGC 1 cut(s) 22
Hin1II CATG 2 cut(s) 893, 917
Hin6I GCGC 1 cut(s) 20
HinP1I GCGC 1 cut(s) 20
HincII GTYRAC 1 cut(s) 202
HindII GTYRAC 1 cut(s) 202
HindIII AAGCTT 1 cut(s) 353
HinfI GANTC 7 cut(s) 140, 496, 728, 886, 1256, 1277, 1313
HpaII CCGG 3 cut(s) 500, 506, 743
HphI GGTGA 8 cut(s) 160, 283, 610, 818, 829, 1064, 1076, 1145
Hpy166II GTNNAC 4 cut(s) 82, 202, 511, 964
Hpy188I TCNGA 3 cut(s) 145, 250, 286
Hpy188III TCNNGA 6 cut(s) 307, 349, 367, 574, 761, 890
Hpy8I GTNNAC 4 cut(s) 82, 202, 511, 964
Hpy99I CGWCG 4 cut(s) 149, 272, 275, 1264
HpyCH4III ACNGT 3 cut(s) 67, 1007, 1186
HpyCH4IV ACGT 2 cut(s) 378, 587
HpyCH4V TGCA 2 cut(s) 115, 517
HpyF10VI GCNNNNNNNGC 7 cut(s) 83, 361, 445, 454, 653, 739, 918
HpyF3I CTNAG 2 cut(s) 1089, 1107
HpySE526I ACGT 2 cut(s) 378, 587
Hsp92II CATG 2 cut(s) 893, 917
HspAI GCGC 1 cut(s) 20
Kzo9I GATC 2 cut(s) 54, 130
LguI GCTCTTC 1 cut(s) 1355
LmnI GCTCC 1 cut(s) 535
Lsp1109I GCAGC 3 cut(s) 99, 441, 529
MaeI CTAG 1 cut(s) 161
MaeII ACGT 2 cut(s) 378, 587
MaeIII GTNAC 1 cut(s) 766
MalI GATC 2 cut(s) 56, 132
MboI GATC 2 cut(s) 54, 130
MhlI GDGCHC 3 cut(s) 255, 384, 1367
MluCI AATT 3 cut(s) 526, 688, 1013
MlyI GAGTC 2 cut(s) 1286, 1322
MmeI TCCRAC 3 cut(s) 168, 309, 1300
MroXI GAANNNNTTC 1 cut(s) 476
MseI TTAA 4 cut(s) 174, 483, 660, 1112
MslI CAYNNNNRTG 1 cut(s) 335
MspCI CTTAAG 1 cut(s) 173
MspI CCGG 3 cut(s) 500, 506, 743
MspR9I CCNGG 1 cut(s) 137
Mva1269I GAATGC 1 cut(s) 649
MvaI CCWGG 1 cut(s) 137
MvnI CGCG 1 cut(s) 198
MwoI GCNNNNNNNGC 7 cut(s) 83, 361, 445, 454, 653, 739, 918
NdeII GATC 2 cut(s) 54, 130
NlaIII CATG 2 cut(s) 893, 917
NlaIV GGNNCC 4 cut(s) 277, 504, 759, 1180
NmuCI GTSAC 1 cut(s) 766
NspV TTCGAA 1 cut(s) 359
PaeR7I CTCGAG 1 cut(s) 1266
PagI TCATGA 1 cut(s) 889
PaqCI CACCTGC 1 cut(s) 529
PciSI GCTCTTC 1 cut(s) 1355
PctI GAATGC 1 cut(s) 649
PdmI GAANNNNTTC 1 cut(s) 476
PfeI GAWTC 5 cut(s) 140, 496, 728, 886, 1256
PflMI CCANNNNNTGG 1 cut(s) 1007
PfoI TCCNGGA 1 cut(s) 135
PkrI GCNGC 6 cut(s) 7, 114, 456, 519, 655, 1408
Ple19I CGATCG 1 cut(s) 57
PleI GAGTC 2 cut(s) 1285, 1321
PpsI GAGTC 2 cut(s) 1285, 1321
Ppu21I YACGTR 1 cut(s) 379
PshAI GACNNNNGTC 1 cut(s) 280
PshBI ATTAAT 1 cut(s) 660
Psp124BI GAGCTC 1 cut(s) 255
Psp6I CCWGG 1 cut(s) 135
PspGI CCWGG 1 cut(s) 135
PspN4I GGNNCC 4 cut(s) 277, 504, 759, 1180
PspPI GGNCC 1 cut(s) 275
PspXI VCTCGAGB 1 cut(s) 1266
PstI CTGCAG 1 cut(s) 117
PvuI CGATCG 1 cut(s) 57
RsaI GTAC 1 cut(s) 580
RsaNI GTAC 1 cut(s) 579
RseI CAYNNNNRTG 1 cut(s) 335
SacI GAGCTC 1 cut(s) 255
SapI GCTCTTC 1 cut(s) 1355
SaqAI TTAA 4 cut(s) 174, 483, 660, 1112
SatI GCNGC 6 cut(s) 6, 113, 455, 518, 654, 1407
Sau3AI GATC 2 cut(s) 54, 130
Sau96I GGNCC 1 cut(s) 275
SchI GAGTC 2 cut(s) 1286, 1322
ScrFI CCNGG 1 cut(s) 137
SduI GDGCHC 3 cut(s) 255, 384, 1367
SfcI CTRYAG 1 cut(s) 113
Sfr274I CTCGAG 1 cut(s) 1266
SfuI TTCGAA 1 cut(s) 359
SinI GGWCC 1 cut(s) 275
SlaI CTCGAG 1 cut(s) 1266
SmiMI CAYNNNNRTG 1 cut(s) 335
SmlI CTYRAG 4 cut(s) 173, 365, 574, 1266
SmoI CTYRAG 4 cut(s) 173, 365, 574, 1266
Sse9I AATT 3 cut(s) 526, 688, 1013
SsiI CCGC 9 cut(s) 6, 33, 70, 100, 185, 439, 647, 653, 755
SspMI CTAG 1 cut(s) 161
SstI GAGCTC 1 cut(s) 255
StyD4I CCNGG 1 cut(s) 135
StyI CCWWGG 1 cut(s) 294
TaaI ACNGT 3 cut(s) 67, 1007, 1186
TaiI ACGT 2 cut(s) 381, 590
TaqI TCGA 5 cut(s) 53, 306, 359, 617, 1267
TasI AATT 3 cut(s) 526, 688, 1013
TatI WGTACW 1 cut(s) 578
TauI GCSGC 2 cut(s) 8, 656
TfiI GAWTC 5 cut(s) 140, 496, 728, 886, 1256
Tru1I TTAA 4 cut(s) 174, 483, 660, 1112
Tru9I TTAA 4 cut(s) 174, 483, 660, 1112
TscAI CASTG 2 cut(s) 1012, 1189
TseFI GTSAC 1 cut(s) 766
TseI GCWGC 4 cut(s) 112, 454, 517, 1406
Tsp45I GTSAC 1 cut(s) 766
TspDTI ATGAA 6 cut(s) 292, 701, 878, 906, 1011, 1365
TspGWI ACGGA 1 cut(s) 288
TspRI CASTG 2 cut(s) 1012, 1189
Van91I CCANNNNNTGG 1 cut(s) 1007
Vha464I CTTAAG 1 cut(s) 173
VpaK11BI GGWCC 1 cut(s) 275
VspI ATTAAT 1 cut(s) 660
XagI CCTNNNNNAGG 2 cut(s) 293, 1382
XhoI CTCGAG 1 cut(s) 1266
XmnI GAANNNNTTC 1 cut(s) 476
XspI CTAG 1 cut(s) 161
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.