Rh4DG225000

Ribosomal L1 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4D
OG0004127
Physical Location & Seq
Forward (+)
42927896 .. 42929460
1565 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4DG225000.1

Sequence Viewer

Length: 1110 bp
ATGGCCAAACACAAGACCAAACACATAAACAAGTTAAAACCCTGGAACGCCAACTTTCTTCTCCCTCTCTTAAACCCCCAGCTTTCCATGGCCGCGACCACCGCAGCTCCTCCACCTTCCAAATCCACACCCACCAAACTCAACCCCAAGACCGTACGAAAAGCCGTCAATGCCCTCCTCAAATGGCGAAATTCCCAGACCCAGAACCCCGACGATCTCTTAGACTCCTCCGACGAATTCACCTACCTCGTCCTCACCCTCAAGAAAATCCCACCAAAGGGTCGCGTCAACGCCTTCAAAATCCCTCTCCCAAATCCCCTCCTCTCCGAATTCACCCAGTTCTGCCTGATCTACGACGACGGCCCCAAGTCCAAGCTCACCAAGGACCACATCGACAAGAGGATCAAAACCCACAACTTACCCATCACCAAAACACTCAAGCTCTCCAAGCTCAAGTCCGATTACAAGTCTTTTGAGGCCAAGCGGAAGCTGATGGAGTCGTATGATGCGTTTCTGGCTGATAAGCAGATTGTGCCGTTGCTTCCCAGGCTTTTGGGGAAGCAGTTCTTCAAGAAGAAGAGGATGATTCCGGTGCCGGTGGACTTGCAACAGAAGAACTGGAAGGAGCAGGTGGATAGGGTTATCCAGTCGGCGATGTTGTTCTTGAGTACTGGGACTTGTAGTGTTGTGAGGGTGGCCAGGACTTCGATGAGTGCTGAGCAGATTGTTGAGAATGTGGTTGCCGCGGTTGATGGGATTGTGGAGATTGTTCCGAGGAATTGGGGCGGTGTGAGGTCGTTTCATTTGAAGCTGTTGGAGTCACTTGCGCTGCCGGTGTATCAGGCTGTGCCGGATGAGACATTGTTGAAGATTGAGGGAGTTAAGGGGAGCGAGAAAGAGGTTGTGAAGAATGAGAATAAGGATTTGAAGAATGAGAAAGTGGGTAAGAAGAAGGGTAGGATTAGTGACGTGAGGTATATGGATAGAAATGTAGGGGAGGTGCTTGATGAGGATGAGTTAGGTGATGGTGTCATTGTCGACAAGGAGAAGAGGAAGAGTGAAGACGCAAAGCTGAAGAAAGCTAAGAGGAGTAAGAATGCAGCAGAGTGA

Protein Analysis

369

Amino Acids

41.7

Weight (kDa)

9.79

Isoelectric Point (pI)

32.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L1 PF00687 78 - 274 8.9e-44 Ribosomal protein L1p/L10e family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 619
Acc36I ACCTGC 1 cut(s) 619
AccB1I GGYRCC 1 cut(s) 592
AccI GTMKAC 1 cut(s) 1038
AccII CGCG 3 cut(s) 95, 285, 746
AciI CCGC 6 cut(s) 93, 102, 484, 744, 746, 786
AclWI GGATC 1 cut(s) 410
AcoI YGGCCR 3 cut(s) 3, 90, 696
AcsI RAATTY 3 cut(s) 190, 236, 329
AcuI CTGAAG 1 cut(s) 1094
AfaI GTAC 2 cut(s) 156, 670
AfiI CCNNNNNNNGG 2 cut(s) 277, 278
AgsI TTSAA 5 cut(s) 298, 571, 808, 868, 928
AjiI CACGTC 1 cut(s) 970
AjnI CCWGG 3 cut(s) 41, 545, 698
AluBI AGCT 9 cut(s) 82, 107, 376, 442, 451, 490, 811, 1072, 1082
AluI AGCT 9 cut(s) 82, 107, 376, 442, 451, 490, 811, 1072, 1082
Alw26I GTCTC 1 cut(s) 851
AlwI GGATC 1 cut(s) 410
AoxI GGCC 5 cut(s) 3, 90, 361, 477, 696
ApeKI GCWGC 3 cut(s) 104, 829, 1100
ApoI RAATTY 3 cut(s) 190, 236, 329
Asp700I GAANNNNTTC 1 cut(s) 563
AspLEI GCGC 1 cut(s) 829
AspS9I GGNCC 2 cut(s) 362, 385
AsuHPI GGTGA 6 cut(s) 232, 247, 325, 370, 418, 1034
AvaII GGWCC 1 cut(s) 385
BalI TGGCCA 2 cut(s) 5, 698
BanI GGYRCC 1 cut(s) 592
BbsI GAAGAC 1 cut(s) 1068
BbvI GCAGC 2 cut(s) 116, 816
BccI CCATC 4 cut(s) 431, 487, 746, 1019
BceAI ACGGC 3 cut(s) 149, 376, 520
BciT130I CCWGG 3 cut(s) 43, 547, 700
BcoDI GTCTC 1 cut(s) 851
BfuAI ACCTGC 1 cut(s) 619
BisI GCNGC 5 cut(s) 93, 105, 744, 830, 1101
BlpI GCTNAGC 1 cut(s) 717
BlsI GCNGC 5 cut(s) 94, 106, 745, 831, 1102
BmcAI AGTACT 1 cut(s) 670
Bme1390I CCNGG 3 cut(s) 43, 547, 700
Bme18I GGWCC 1 cut(s) 385
BmgBI CACGTC 1 cut(s) 970
BmgT120I GGNCC 2 cut(s) 362, 385
BmiI GGNNCC 2 cut(s) 364, 594
BmrFI CCNGG 3 cut(s) 43, 547, 700
BmrI ACTGGG 2 cut(s) 331, 681
BmsI GCATC 1 cut(s) 496
BmuI ACTGGG 2 cut(s) 331, 681
BpiI GAAGAC 1 cut(s) 1068
Bpu1102I GCTNAGC 1 cut(s) 717
BpuEI CTTGAG 4 cut(s) 245, 422, 437, 685
BsaJI CCNNGG 6 cut(s) 41, 87, 381, 545, 744, 773
BsaWI WCCGGW 1 cut(s) 589
BsaXI ACNNNNNCTCC 2 cut(s) 91, 121
Bsc4I CCNNNNNNNGG 2 cut(s) 277, 278
Bse118I RCCGGY 2 cut(s) 595, 832
Bse1I ACTGG 4 cut(s) 337, 623, 646, 676
BseBI CCWGG 3 cut(s) 43, 547, 700
BseDI CCNNGG 6 cut(s) 41, 87, 381, 545, 744, 773
BseGI GGATG 3 cut(s) 588, 859, 1018
BseLI CCNNNNNNNGG 2 cut(s) 277, 278
BseMII CTCAG 1 cut(s) 708
BseNI ACTGG 4 cut(s) 337, 623, 646, 676
BseRI GAGGAG 5 cut(s) 99, 167, 217, 311, 1102
BseXI GCAGC 2 cut(s) 116, 816
BseYI CCCAGC 1 cut(s) 78
Bsh1236I CGCG 3 cut(s) 95, 285, 746
BshFI GGCC 5 cut(s) 5, 92, 363, 479, 698
BshNI GGYRCC 1 cut(s) 592
BsiSI CCGG 4 cut(s) 590, 596, 833, 851
BsiWI CGTACG 1 cut(s) 154
BslFI GGGAC 1 cut(s) 688
BslI CCNNNNNNNGG 2 cut(s) 277, 278
BsmAI GTCTC 1 cut(s) 851
BsmFI GGGAC 1 cut(s) 688
BsmI GAATGC 1 cut(s) 1102
BsnI GGCC 5 cut(s) 5, 92, 363, 479, 698
Bsp143I GATC 3 cut(s) 214, 348, 402
Bsp1720I GCTNAGC 1 cut(s) 717
Bsp19I CCATGG 1 cut(s) 87
BspACI CCGC 6 cut(s) 93, 102, 484, 744, 746, 786
BspANI GGCC 5 cut(s) 5, 92, 363, 479, 698
BspCNI CTCAG 1 cut(s) 709
BspFNI CGCG 3 cut(s) 95, 285, 746
BspLI GGNNCC 2 cut(s) 364, 594
BspMI ACCTGC 1 cut(s) 619
BspPI GGATC 1 cut(s) 410
BspT107I GGYRCC 1 cut(s) 592
BsrFI RCCGGY 2 cut(s) 595, 832
BsrI ACTGG 4 cut(s) 337, 623, 646, 676
BssAI RCCGGY 2 cut(s) 595, 832
BssECI CCNNGG 6 cut(s) 41, 87, 381, 545, 744, 773
BssMI GATC 3 cut(s) 214, 348, 402
BssT1I CCWWGG 2 cut(s) 87, 381
Bst2UI CCWGG 3 cut(s) 43, 547, 700
Bst4CI ACNGT 1 cut(s) 154
Bst6I CTCTTC 3 cut(s) 572, 1043, 1049
BstAPI GCANNNNNTGC 1 cut(s) 532
BstDEI CTNAG 3 cut(s) 220, 717, 1083
BstDSI CCRYGG 2 cut(s) 87, 744
BstF5I GGATG 3 cut(s) 588, 859, 1018
BstFNI CGCG 3 cut(s) 95, 285, 746
BstHHI GCGC 1 cut(s) 829
BstKTI GATC 3 cut(s) 217, 351, 405
BstMAI GTCTC 1 cut(s) 851
BstMBI GATC 3 cut(s) 214, 348, 402
BstMWI GCNNNNNNNGC 6 cut(s) 101, 170, 448, 515, 532, 547
BstNI CCWGG 3 cut(s) 43, 547, 700
BstSCI CCNGG 3 cut(s) 41, 545, 698
BstUI CGCG 3 cut(s) 95, 285, 746
BstV1I GCAGC 2 cut(s) 116, 816
BstV2I GAAGAC 1 cut(s) 1068
BstXI CCANNNNNNTGG 1 cut(s) 553
BsuRI GGCC 5 cut(s) 5, 92, 363, 479, 698
BtgI CCRYGG 2 cut(s) 87, 744
BtgZI GCGATG 1 cut(s) 668
BtrI CACGTC 1 cut(s) 970
BtsCI GGATG 3 cut(s) 588, 859, 1018
BveI ACCTGC 1 cut(s) 619
CfoI GCGC 1 cut(s) 829
Cfr10I RCCGGY 2 cut(s) 595, 832
Cfr13I GGNCC 2 cut(s) 362, 385
Cfr42I CCGCGG 1 cut(s) 747
CseI GACGC 2 cut(s) 274, 1073
Csp6I GTAC 2 cut(s) 155, 669
CviAII CATG 1 cut(s) 88
CviQI GTAC 2 cut(s) 155, 669
DdeI CTNAG 3 cut(s) 220, 717, 1083
DpnI GATC 3 cut(s) 216, 350, 404
DpnII GATC 3 cut(s) 214, 348, 402
EaeI YGGCCR 3 cut(s) 3, 90, 696
Eam1104I CTCTTC 3 cut(s) 572, 1043, 1049
EarI CTCTTC 3 cut(s) 572, 1043, 1049
Eco130I CCWWGG 2 cut(s) 87, 381
Eco47I GGWCC 1 cut(s) 385
Eco57I CTGAAG 1 cut(s) 1094
EcoRI GAATTC 2 cut(s) 236, 329
EcoRII CCWGG 3 cut(s) 41, 545, 698
EcoT14I CCWWGG 2 cut(s) 87, 381
ErhI CCWWGG 2 cut(s) 87, 381
FaeI CATG 1 cut(s) 91
FaiI YATR 5 cut(s) 26, 89, 504, 978, 980
FalI AAGNNNNNCTT 2 cut(s) 551, 583
FaqI GGGAC 1 cut(s) 688
FatI CATG 1 cut(s) 87
FblI GTMKAC 1 cut(s) 1038
Fnu4HI GCNGC 5 cut(s) 93, 105, 744, 830, 1101
FokI GGATG 3 cut(s) 595, 866, 1025
Fsp4HI GCNGC 5 cut(s) 93, 105, 744, 830, 1101
GlaI GCGC 1 cut(s) 828
GluI GCNGC 5 cut(s) 93, 105, 744, 830, 1101
GsaI CCCAGC 1 cut(s) 82
HaeIII GGCC 5 cut(s) 5, 92, 363, 479, 698
HapII CCGG 4 cut(s) 590, 596, 833, 851
HgaI GACGC 2 cut(s) 274, 1073
HhaI GCGC 1 cut(s) 829
Hin1II CATG 1 cut(s) 91
Hin6I GCGC 1 cut(s) 827
HinP1I GCGC 1 cut(s) 827
HincII GTYRAC 2 cut(s) 289, 1039
HindII GTYRAC 2 cut(s) 289, 1039
HinfI GANTC 4 cut(s) 224, 497, 586, 818
HpaII CCGG 4 cut(s) 590, 596, 833, 851
HphI GGTGA 6 cut(s) 232, 247, 325, 370, 418, 1034
Hpy166II GTNNAC 3 cut(s) 289, 601, 1039
Hpy188I TCNGA 4 cut(s) 232, 328, 460, 774
Hpy188III TCNNGA 3 cut(s) 262, 571, 664
Hpy8I GTNNAC 3 cut(s) 289, 601, 1039
Hpy99I CGWCG 4 cut(s) 215, 236, 359, 362
HpyAV CCTTC 4 cut(s) 126, 304, 616, 946
HpyCH4III ACNGT 1 cut(s) 154
HpyCH4IV ACGT 1 cut(s) 969
HpyCH4V TGCA 2 cut(s) 607, 1100
HpyF10VI GCNNNNNNNGC 6 cut(s) 101, 170, 448, 515, 532, 547
HpyF3I CTNAG 3 cut(s) 220, 717, 1083
HpySE526I ACGT 1 cut(s) 969
Hsp92II CATG 1 cut(s) 91
HspAI GCGC 1 cut(s) 827
KspI CCGCGG 1 cut(s) 747
Kzo9I GATC 3 cut(s) 214, 348, 402
LmnI GCTCC 3 cut(s) 112, 625, 888
Lsp1109I GCAGC 2 cut(s) 116, 816
LweI GCATC 1 cut(s) 496
MaeII ACGT 1 cut(s) 969
MaeIII GTNAC 2 cut(s) 819, 965
MalI GATC 3 cut(s) 216, 350, 404
MboI GATC 3 cut(s) 214, 348, 402
MlsI TGGCCA 2 cut(s) 5, 698
MluCI AATT 4 cut(s) 190, 236, 329, 778
MluNI TGGCCA 2 cut(s) 5, 698
MlyI GAGTC 3 cut(s) 218, 506, 827
MmeI TCCRAC 2 cut(s) 255, 795
Mox20I TGGCCA 2 cut(s) 5, 698
MroXI GAANNNNTTC 1 cut(s) 563
MscI TGGCCA 2 cut(s) 5, 698
MseI TTAA 3 cut(s) 35, 71, 882
Msp20I TGGCCA 2 cut(s) 5, 698
MspA1I CMGCKG 1 cut(s) 746
MspI CCGG 4 cut(s) 590, 596, 833, 851
MspR9I CCNGG 3 cut(s) 43, 547, 700
Mva1269I GAATGC 1 cut(s) 1102
MvaI CCWGG 3 cut(s) 43, 547, 700
MvnI CGCG 3 cut(s) 95, 285, 746
MwoI GCNNNNNNNGC 6 cut(s) 101, 170, 448, 515, 532, 547
NcoI CCATGG 1 cut(s) 87
NdeII GATC 3 cut(s) 214, 348, 402
NlaIII CATG 1 cut(s) 91
NlaIV GGNNCC 2 cut(s) 364, 594
NmuCI GTSAC 2 cut(s) 819, 965
PaqCI CACCTGC 1 cut(s) 619
PcsI WCGNNNNNNNCGW 1 cut(s) 506
PctI GAATGC 1 cut(s) 1102
PdmI GAANNNNTTC 1 cut(s) 563
PfeI GAWTC 1 cut(s) 586
Pfl23II CGTACG 1 cut(s) 154
PkrI GCNGC 5 cut(s) 94, 106, 745, 831, 1102
PleI GAGTC 3 cut(s) 218, 505, 826
PpsI GAGTC 3 cut(s) 218, 505, 826
Psp6I CCWGG 3 cut(s) 41, 545, 698
PspFI CCCAGC 1 cut(s) 78
PspGI CCWGG 3 cut(s) 41, 545, 698
PspLI CGTACG 1 cut(s) 154
PspN4I GGNNCC 2 cut(s) 364, 594
PspPI GGNCC 2 cut(s) 362, 385
RsaI GTAC 2 cut(s) 156, 670
RsaNI GTAC 2 cut(s) 155, 669
SacII CCGCGG 1 cut(s) 747
SalI GTCGAC 1 cut(s) 1037
SaqAI TTAA 3 cut(s) 35, 71, 882
SatI GCNGC 5 cut(s) 93, 105, 744, 830, 1101
Sau3AI GATC 3 cut(s) 214, 348, 402
Sau96I GGNCC 2 cut(s) 362, 385
ScaI AGTACT 1 cut(s) 670
SchI GAGTC 3 cut(s) 218, 506, 827
ScrFI CCNGG 3 cut(s) 43, 547, 700
SfaNI GCATC 1 cut(s) 496
Sfr303I CCGCGG 1 cut(s) 747
SgrBI CCGCGG 1 cut(s) 747
SinI GGWCC 1 cut(s) 385
SmlI CTYRAG 4 cut(s) 260, 437, 452, 664
SmoI CTYRAG 4 cut(s) 260, 437, 452, 664
Sse9I AATT 4 cut(s) 190, 236, 329, 778
SsiI CCGC 6 cut(s) 93, 102, 484, 744, 746, 786
StyD4I CCNGG 3 cut(s) 41, 545, 698
StyI CCWWGG 2 cut(s) 87, 381
TaaI ACNGT 1 cut(s) 154
TaiI ACGT 1 cut(s) 972
TaqI TCGA 3 cut(s) 393, 707, 1038
TasI AATT 4 cut(s) 190, 236, 329, 778
TatI WGTACW 1 cut(s) 668
TauI GCSGC 2 cut(s) 95, 746
TfiI GAWTC 1 cut(s) 586
Tru1I TTAA 3 cut(s) 35, 71, 882
Tru9I TTAA 3 cut(s) 35, 71, 882
TseFI GTSAC 2 cut(s) 819, 965
TseI GCWGC 3 cut(s) 104, 829, 1100
Tsp45I GTSAC 2 cut(s) 819, 965
TspDTI ATGAA 1 cut(s) 791
VpaK11BI GGWCC 1 cut(s) 385
XapI RAATTY 3 cut(s) 190, 236, 329
XmiI GTMKAC 1 cut(s) 1038
XmnI GAANNNNTTC 1 cut(s) 563
ZrmI AGTACT 1 cut(s) 670
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.