MD09G1152200.v1.1

Ribosomal L1 domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0004127
Physical Location & Seq
Forward (+)
12050393 .. 12051892
1500 bp
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UTR
Exon/CDS
Intron
MD09G1152200.v1.1.491

Sequence Viewer

Length: 1374 bp
ATGGCCGCCACCACTCCAGCGCCTCCACCTTCCACCACAAAACCCACCAAATCGATCACCAAGACGGTGAGGAAAGCCGTAAACGCCCTCATCAAATGGCGGAACGACAAGCTGCAGACCCAGAACCCCGATCTCTTGGAATCCGACGAGTTCGCCTACCTGGTTCTCACCCTCAAGAAAACCCCCCCAAAGGGTCGCGTCAACGCCTACAAAATCCCCCTCCCAAATCCCCTGCATTCCCAACTCTCCGAGCTCTGCCTCATATACGACGACGGACCCAAGTCCAACCTCACGAAGGACTTCATCGAGAAGAAAATCAAGGCCGAAAACATAACCGTGTCGAAAATCTTGAAGCTTTCGAAGCTCAAGAGCGATTACGTGCCCTTTGAGGCCAAGAGGAAACTGATGTATTCGTATGATATGTTTCTGGCTGATAAGCGGATTGTGCCGTTGCTGCCAAAGTTTTTGGGGAAGCATTTCTTTAAGAAGAAGAAGATTCCGGTGCCGGTGGACCTGGAGCACAAGAATTGGAAGGAGCAGGTGGATAAGATTTGTGGGTCGGCTTTGTTGTACTTGAGTACAGGGACGTGTAGCGTGGTGAGGGTAGCGAAGGTTTCGATGAGCGTTGATGAGATTGTGGCGAATGTGGTTGCGGCGATTGATGGGATTGTGGAGATGGTGCCTAAGAACTGGGGAGGTGCGGTTCCGGATGTGACATTGAAGATTGAGGAAGAGAAGGTGGTTGAGGAAGGTGCAGAGGAGGTGAAGGAGGTTGTTAAGAATGAGCGCAAGGATTTGAAGAGTGAGAAGAAGAGTAAGAAGAAGGGTAGGATTCATGAAGTGAGGTATTTGGATAGCAATGTCGGCGAGGTGCTTGATGATGATGAATTGGGTATTCTTGGTGATGTGGATATTGGGGAAGGGAAGCAAAGCGAGAATGATGAACCGGGTAGTGGCGAATTGGGGAAGAAGAAGAGGAAGAAGGAAAAGGTTGGTGGTGAGTCTAAGGGTGAGAAAAGGTTGAAGAAGTCGGCTAAGGCGAAAGATGATGCTGAGTTAAATGGGGAAAAGGTTCTTGGTGAGAAAAACAGTGAGAAGAAGTTGAAAGGGTCGGCAAAGGCAGAAGAAGAGGACGGTGCCATTGTCAAGAATGAGAAGGATGGGTTGTCTTCGAAAGAGAAGAAAAAATATGCTACAAAGAAGAAAGCAGATGATTTGTCGGCCAAAGGCGAAGAGTCTGTTGGAAAGAAGGAGAAGAGGAAGAGTGAAGAGTCTGCTGGAAAGAAGGAGAAGGGGAAGAGTGAAGATGAAAAGTTGAAGGGCACAGAAGCAAAGCTGAAGAAGTCTAAGAGAAGTAAGAAAGCAGCAGAGTAA

Protein Analysis

458

Amino Acids

50.74

Weight (kDa)

9.43

Isoelectric Point (pI)

29.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L1 PF00687 48 - 241 7.2e-43 Ribosomal protein L1p/L10e family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 529
Acc36I ACCTGC 1 cut(s) 529
AccB1I GGYRCC 3 cut(s) 502, 679, 1136
AccII CGCG 1 cut(s) 198
AccIII TCCGGA 1 cut(s) 706
AciI CCGC 5 cut(s) 6, 100, 439, 653, 701
AcoI YGGCCR 2 cut(s) 3, 1221
AcuI CTGAAG 1 cut(s) 1358
AfaI GTAC 2 cut(s) 572, 580
AfiI CCNNNNNNNGG 4 cut(s) 190, 191, 295, 953
AflIII ACRYGT 1 cut(s) 587
AgsI TTSAA 6 cut(s) 352, 721, 799, 1024, 1105, 1318
AjiI CACGTC 1 cut(s) 588
AjnI CCWGG 2 cut(s) 159, 513
AjuI GAANNNNNNNTTGG 4 cut(s) 1059, 1091, 1224, 1256
AloI GAACNNNNNNTCC 2 cut(s) 687, 719
AluBI AGCT 5 cut(s) 112, 253, 355, 364, 1336
AluI AGCT 5 cut(s) 112, 253, 355, 364, 1336
Alw21I GWGCWC 2 cut(s) 255, 522
Aor13HI TCCGGA 1 cut(s) 706
AoxI GGCC 4 cut(s) 3, 321, 390, 1221
ApeKI GCWGC 3 cut(s) 112, 454, 1364
Asp700I GAANNNNTTC 3 cut(s) 299, 476, 1071
AspLEI GCGC 2 cut(s) 22, 789
AspS9I GGNCC 2 cut(s) 275, 511
AsuC2I CCSGG 1 cut(s) 948
AsuHPI GGTGA 9 cut(s) 49, 79, 160, 610, 775, 914, 1010, 1022, 1091
AsuII TTCGAA 2 cut(s) 359, 1172
AvaII GGWCC 2 cut(s) 275, 511
BaeGI GKGCMC 2 cut(s) 384, 1325
BanI GGYRCC 3 cut(s) 502, 679, 1136
BanII GRGCYC 1 cut(s) 255
BbsI GAAGAC 1 cut(s) 1161
Bbv12I GWGCWC 2 cut(s) 255, 522
BbvI GCAGC 2 cut(s) 99, 441
BccI CCATC 3 cut(s) 656, 670, 1154
BceAI ACGGC 2 cut(s) 62, 433
BciT130I CCWGG 2 cut(s) 161, 515
BcnI CCSGG 1 cut(s) 948
BfmI CTRYAG 1 cut(s) 113
BfoI RGCGCY 1 cut(s) 23
BfuAI ACCTGC 1 cut(s) 529
BisI GCNGC 5 cut(s) 6, 113, 455, 654, 1365
BlsI GCNGC 5 cut(s) 7, 114, 456, 655, 1366
Bme1390I CCNGG 3 cut(s) 161, 515, 948
Bme18I GGWCC 2 cut(s) 275, 511
BmgBI CACGTC 1 cut(s) 588
BmgT120I GGNCC 2 cut(s) 275, 511
BmiI GGNNCC 5 cut(s) 277, 504, 681, 705, 1138
BmrFI CCNGG 3 cut(s) 161, 515, 948
BmrI ACTGGG 1 cut(s) 700
BmsI GCATC 1 cut(s) 1039
BmuI ACTGGG 1 cut(s) 700
BoxI GACNNNNGTC 1 cut(s) 280
BpiI GAAGAC 1 cut(s) 1161
BpmI CTGGAG 1 cut(s) 536
Bpu10I CCTNAGC 1 cut(s) 1035
Bpu14I TTCGAA 2 cut(s) 359, 1172
BpuEI CTTGAG 3 cut(s) 158, 350, 595
BpuMI CCSGG 1 cut(s) 948
Bsa29I ATCGAT 1 cut(s) 53
BsaAI YACGTR 1 cut(s) 379
BsaWI WCCGGW 2 cut(s) 499, 706
BsaXI ACNNNNNCTCC 2 cut(s) 687, 717
Bsc4I CCNNNNNNNGG 4 cut(s) 190, 191, 295, 953
Bse118I RCCGGY 1 cut(s) 505
Bse1I ACTGG 1 cut(s) 695
Bse3DI GCAATG 1 cut(s) 865
BseAI TCCGGA 1 cut(s) 706
BseBI CCWGG 2 cut(s) 161, 515
BseCI ATCGAT 1 cut(s) 53
BseGI GGATG 2 cut(s) 715, 1165
BseLI CCNNNNNNNGG 4 cut(s) 190, 191, 295, 953
BseMI GCAATG 1 cut(s) 865
BseMII CTCAG 1 cut(s) 1044
BseNI ACTGG 1 cut(s) 695
BseRI GAGGAG 1 cut(s) 773
BseSI GKGCMC 2 cut(s) 384, 1325
BseXI GCAGC 2 cut(s) 99, 441
BsgI GTGCAG 1 cut(s) 774
Bsh1236I CGCG 1 cut(s) 198
BshFI GGCC 4 cut(s) 5, 323, 392, 1223
BshNI GGYRCC 3 cut(s) 502, 679, 1136
BshVI ATCGAT 1 cut(s) 53
BsiHKAI GWGCWC 2 cut(s) 255, 522
BsiSI CCGG 4 cut(s) 500, 506, 707, 947
BslFI GGGAC 1 cut(s) 598
BslI CCNNNNNNNGG 4 cut(s) 190, 191, 295, 953
BsmFI GGGAC 1 cut(s) 598
BsmI GAATGC 1 cut(s) 235
BsnI GGCC 4 cut(s) 5, 323, 392, 1223
Bsp119I TTCGAA 2 cut(s) 359, 1172
Bsp1286I GDGCHC 4 cut(s) 255, 384, 522, 1325
Bsp13I TCCGGA 1 cut(s) 706
Bsp143I GATC 2 cut(s) 54, 130
BspACI CCGC 5 cut(s) 6, 100, 439, 653, 701
BspANI GGCC 4 cut(s) 5, 323, 392, 1223
BspCNI CTCAG 1 cut(s) 1045
BspDI ATCGAT 1 cut(s) 53
BspEI TCCGGA 1 cut(s) 706
BspFNI CGCG 1 cut(s) 198
BspHI TCATGA 1 cut(s) 835
BspLI GGNNCC 5 cut(s) 277, 504, 681, 705, 1138
BspMAI CTGCAG 1 cut(s) 117
BspMI ACCTGC 1 cut(s) 529
BspT104I TTCGAA 2 cut(s) 359, 1172
BspT107I GGYRCC 3 cut(s) 502, 679, 1136
BsrDI GCAATG 1 cut(s) 865
BsrFI RCCGGY 1 cut(s) 505
BsrI ACTGG 1 cut(s) 695
BssAI RCCGGY 1 cut(s) 505
BssMI GATC 2 cut(s) 54, 130
Bst2UI CCWGG 2 cut(s) 161, 515
Bst4CI ACNGT 4 cut(s) 67, 337, 1091, 1136
BstBAI YACGTR 1 cut(s) 379
BstBI TTCGAA 2 cut(s) 359, 1172
BstDEI CTNAG 5 cut(s) 684, 1005, 1035, 1053, 1347
BstENI CCTNNNNNAGG 1 cut(s) 293
BstF5I GGATG 2 cut(s) 715, 1165
BstFNI CGCG 1 cut(s) 198
BstH2I RGCGCY 1 cut(s) 23
BstHHI GCGC 2 cut(s) 22, 789
BstKTI GATC 2 cut(s) 57, 133
BstMBI GATC 2 cut(s) 54, 130
BstMWI GCNNNNNNNGC 5 cut(s) 83, 361, 445, 454, 864
BstNI CCWGG 2 cut(s) 161, 515
BstPAI GACNNNNGTC 1 cut(s) 280
BstSCI CCNGG 3 cut(s) 159, 513, 946
BstSFI CTRYAG 1 cut(s) 113
BstSLI GKGCMC 2 cut(s) 384, 1325
BstUI CGCG 1 cut(s) 198
BstV1I GCAGC 2 cut(s) 99, 441
BstV2I GAAGAC 1 cut(s) 1161
Bsu15I ATCGAT 1 cut(s) 53
BsuRI GGCC 4 cut(s) 5, 323, 392, 1223
BsuTUI ATCGAT 1 cut(s) 53
BtrI CACGTC 1 cut(s) 588
BtsCI GGATG 2 cut(s) 715, 1165
BtsIMutI CAGTG 1 cut(s) 1096
BveI ACCTGC 1 cut(s) 529
CciI TCATGA 1 cut(s) 835
CfoI GCGC 2 cut(s) 22, 789
Cfr10I RCCGGY 1 cut(s) 505
Cfr13I GGNCC 2 cut(s) 275, 511
ClaI ATCGAT 1 cut(s) 53
CseI GACGC 1 cut(s) 187
CsiI ACCWGGT 1 cut(s) 159
Csp6I GTAC 2 cut(s) 571, 579
CviAII CATG 1 cut(s) 836
CviQI GTAC 2 cut(s) 571, 579
DdeI CTNAG 5 cut(s) 684, 1005, 1035, 1053, 1347
DpnI GATC 2 cut(s) 56, 132
DpnII GATC 2 cut(s) 54, 130
EaeI YGGCCR 2 cut(s) 3, 1221
EciI GGCGGA 1 cut(s) 115
Ecl136II GAGCTC 1 cut(s) 253
Eco24I GRGCYC 1 cut(s) 255
Eco47I GGWCC 2 cut(s) 275, 511
Eco53kI GAGCTC 1 cut(s) 253
Eco57I CTGAAG 1 cut(s) 1358
EcoICRI GAGCTC 1 cut(s) 253
EcoNI CCTNNNNNAGG 1 cut(s) 293
EcoRII CCWGG 2 cut(s) 159, 513
EcoT38I GRGCYC 1 cut(s) 255
FaeI CATG 1 cut(s) 839
FaiI YATR 7 cut(s) 263, 265, 332, 417, 422, 837, 1191
FalI AAGNNNNNCTT 2 cut(s) 464, 496
FaqI GGGAC 1 cut(s) 598
FatI CATG 1 cut(s) 835
Fnu4HI GCNGC 5 cut(s) 6, 113, 455, 654, 1365
FokI GGATG 2 cut(s) 722, 1172
FriOI GRGCYC 1 cut(s) 255
Fsp4HI GCNGC 5 cut(s) 6, 113, 455, 654, 1365
GlaI GCGC 2 cut(s) 21, 788
GluI GCNGC 5 cut(s) 6, 113, 455, 654, 1365
GsuI CTGGAG 1 cut(s) 536
HaeII RGCGCY 1 cut(s) 23
HaeIII GGCC 4 cut(s) 5, 323, 392, 1223
HapII CCGG 4 cut(s) 500, 506, 707, 947
HgaI GACGC 1 cut(s) 187
HhaI GCGC 2 cut(s) 22, 789
Hin1II CATG 1 cut(s) 839
Hin6I GCGC 2 cut(s) 20, 787
HinP1I GCGC 2 cut(s) 20, 787
HincII GTYRAC 1 cut(s) 202
HindII GTYRAC 1 cut(s) 202
HindIII AAGCTT 1 cut(s) 353
HinfI GANTC 6 cut(s) 140, 496, 832, 1001, 1235, 1271
HpaII CCGG 4 cut(s) 500, 506, 707, 947
HphI GGTGA 9 cut(s) 49, 79, 160, 610, 775, 914, 1010, 1022, 1091
Hpy166II GTNNAC 3 cut(s) 82, 202, 511
Hpy188I TCNGA 2 cut(s) 145, 250
Hpy188III TCNNGA 8 cut(s) 175, 292, 307, 349, 367, 707, 836, 1147
Hpy8I GTNNAC 3 cut(s) 82, 202, 511
Hpy99I CGWCG 3 cut(s) 149, 272, 275
HpyCH4III ACNGT 4 cut(s) 67, 337, 1091, 1136
HpyCH4IV ACGT 2 cut(s) 378, 587
HpyCH4V TGCA 3 cut(s) 115, 235, 755
HpyF10VI GCNNNNNNNGC 5 cut(s) 83, 361, 445, 454, 864
HpyF3I CTNAG 5 cut(s) 684, 1005, 1035, 1053, 1347
HpySE526I ACGT 2 cut(s) 378, 587
Hsp92II CATG 1 cut(s) 839
HspAI GCGC 2 cut(s) 20, 787
Kpn2I TCCGGA 1 cut(s) 706
Kzo9I GATC 2 cut(s) 54, 130
LmnI GCTCC 2 cut(s) 517, 535
Lsp1109I GCAGC 2 cut(s) 99, 441
LweI GCATC 1 cut(s) 1039
MabI ACCWGGT 1 cut(s) 159
MaeII ACGT 2 cut(s) 378, 587
MaeIII GTNAC 1 cut(s) 712
MalI GATC 2 cut(s) 56, 132
MboI GATC 2 cut(s) 54, 130
MhlI GDGCHC 4 cut(s) 255, 384, 522, 1325
MluCI AATT 3 cut(s) 526, 887, 959
MlyI GAGTC 3 cut(s) 1010, 1244, 1280
MmeI TCCRAC 3 cut(s) 168, 309, 1222
MroI TCCGGA 1 cut(s) 706
MroXI GAANNNNTTC 3 cut(s) 299, 476, 1071
MseI TTAA 3 cut(s) 483, 777, 1058
MslI CAYNNNNRTG 1 cut(s) 335
MspI CCGG 4 cut(s) 500, 506, 707, 947
MspR9I CCNGG 3 cut(s) 161, 515, 948
Mva1269I GAATGC 1 cut(s) 235
MvaI CCWGG 2 cut(s) 161, 515
MvnI CGCG 1 cut(s) 198
MwoI GCNNNNNNNGC 5 cut(s) 83, 361, 445, 454, 864
NciI CCSGG 1 cut(s) 948
NdeII GATC 2 cut(s) 54, 130
NlaIII CATG 1 cut(s) 839
NlaIV GGNNCC 5 cut(s) 277, 504, 681, 705, 1138
NmuCI GTSAC 1 cut(s) 712
NspV TTCGAA 2 cut(s) 359, 1172
PagI TCATGA 1 cut(s) 835
PaqCI CACCTGC 1 cut(s) 529
PcsI WCGNNNNNNNCGW 1 cut(s) 1037
PctI GAATGC 1 cut(s) 235
PdmI GAANNNNTTC 3 cut(s) 299, 476, 1071
PfeI GAWTC 3 cut(s) 140, 496, 832
PkrI GCNGC 5 cut(s) 7, 114, 456, 655, 1366
PleI GAGTC 3 cut(s) 1009, 1243, 1279
PpsI GAGTC 3 cut(s) 1009, 1243, 1279
Ppu21I YACGTR 1 cut(s) 379
PshAI GACNNNNGTC 1 cut(s) 280
Psp124BI GAGCTC 1 cut(s) 255
Psp6I CCWGG 2 cut(s) 159, 513
PspGI CCWGG 2 cut(s) 159, 513
PspN4I GGNNCC 5 cut(s) 277, 504, 681, 705, 1138
PspPI GGNCC 2 cut(s) 275, 511
PstI CTGCAG 1 cut(s) 117
RsaI GTAC 2 cut(s) 572, 580
RsaNI GTAC 2 cut(s) 571, 579
RseI CAYNNNNRTG 1 cut(s) 335
SacI GAGCTC 1 cut(s) 255
SaqAI TTAA 3 cut(s) 483, 777, 1058
SatI GCNGC 5 cut(s) 6, 113, 455, 654, 1365
Sau3AI GATC 2 cut(s) 54, 130
Sau96I GGNCC 2 cut(s) 275, 511
SchI GAGTC 3 cut(s) 1010, 1244, 1280
ScrFI CCNGG 3 cut(s) 161, 515, 948
SduI GDGCHC 4 cut(s) 255, 384, 522, 1325
SexAI ACCWGGT 1 cut(s) 159
SfaNI GCATC 1 cut(s) 1039
SfcI CTRYAG 1 cut(s) 113
SfuI TTCGAA 2 cut(s) 359, 1172
SinI GGWCC 2 cut(s) 275, 511
SmiMI CAYNNNNRTG 1 cut(s) 335
SmlI CTYRAG 3 cut(s) 173, 365, 574
SmoI CTYRAG 3 cut(s) 173, 365, 574
Sse9I AATT 3 cut(s) 526, 887, 959
SsiI CCGC 5 cut(s) 6, 100, 439, 653, 701
SstI GAGCTC 1 cut(s) 255
StyD4I CCNGG 3 cut(s) 159, 513, 946
TaaI ACNGT 4 cut(s) 67, 337, 1091, 1136
TaiI ACGT 2 cut(s) 381, 590
TaqI TCGA 6 cut(s) 53, 306, 341, 359, 617, 1172
TasI AATT 3 cut(s) 526, 887, 959
TatI WGTACW 2 cut(s) 570, 578
TauI GCSGC 2 cut(s) 8, 656
TfiI GAWTC 3 cut(s) 140, 496, 832
Tru1I TTAA 3 cut(s) 483, 777, 1058
Tru9I TTAA 3 cut(s) 483, 777, 1058
TscAI CASTG 1 cut(s) 1096
TseFI GTSAC 1 cut(s) 712
TseI GCWGC 3 cut(s) 112, 454, 1364
Tsp45I GTSAC 1 cut(s) 712
TspDTI ATGAA 6 cut(s) 292, 824, 852, 900, 957, 1323
TspGWI ACGGA 1 cut(s) 288
TspRI CASTG 1 cut(s) 1096
VpaK11BI GGWCC 2 cut(s) 275, 511
XagI CCTNNNNNAGG 1 cut(s) 293
XmnI GAANNNNTTC 3 cut(s) 299, 476, 1071
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.